                         SEQUENCE LISTING

<110>  Rutter, Jared
       Thummel, Carl
       Bricker, Daniel  K
       Orsak, Thomas
 
<120>  COMPOSITIONS AND METHODS FOR MODULATING MITOCHONDRIAL PYRUVATE 
       CARRIER ACTIVITY

<130>  21101.0267P1

<150>  61/645,315
<151>  2012-05-10

<150>  61/664,434
<151>  2012-06-26

<160>  47    

<170>  PatentIn version 3.5

<210>  1
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; forward primer for hsMPC1 exon 1

<400>  1
ccagccccag ccgttttacg gcag                                              24


<210>  2
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; reverse primer for hsMPC1 exon 1

<400>  2
ctgaaaggcg cccactgtca ccg                                               23


<210>  3
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; forward primer for hsMPC1 exon 2

<400>  3
ggcacaaacc acccatgccc agc                                               23


<210>  4
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; reverse primer for hsMPC1 exon 2

<400>  4
gctgtcacag agctcgtgtc taggc                                             25


<210>  5
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; forward primer for hsMPC1 exon 3

<400>  5
gtgtctgggg tcctgggcat tgatttc                                           27


<210>  6
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; reverse primer for hsMPC1 exon 3

<400>  6
gcaagcagga gctctactat gttgaaagtc c                                      31


<210>  7
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; forward primer for hsMPC1 exon 4

<400>  7
cagcctactc ttgctcggaa atatgttcc                                         29


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; reverse primer for hsMPC1 exon 4

<400>  8
ccagtcccgc agcactccct c                                                 21


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; forward primer for hsMPC1 cDNA

<400>  9
gccggggtgt cattggctct                                                   20


<210>  10
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; reverse primer for hsMPC1 cDNA

<400>  10
gtgactcagc agcagctggc aat                                               23


<210>  11
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; forward primer for hsMPC1 ORF

<400>  11
gatcgcggcc gcaccatggc gggcgcgttg                                        30


<210>  12
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; reverse primer for hsMPC1 ORF

<400>  12
gatcggatcc ttatgcagat gccgttttag tcatctc                                37


<210>  13
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; forward primer for mmMPC1 qPCR

<400>  13
gcacggccat ggctggagc                                                    19


<210>  14
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; reverse primer for mmMPC1 qPCR

<400>  14
gcaacagagg gcgaaagtca tccg                                              24


<210>  15
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; forward primer for mmMPC2 qPCR

<400>  15
ccgctttaca accacccggc a                                                 21


<210>  16
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; reverse primer for mmMPC2 qPCR

<400>  16
cagcacacac caatccccat ttca                                              24


<210>  17
<211>  109
<212>  PRT
<213>  Homo sapiens

<400>  17

Met Ala Gly Ala Leu Val Arg Lys Ala Ala Asp Tyr Val Arg Ser Lys 
1               5                   10                  15      


Asp Phe Arg Asp Tyr Leu Met Ser Thr His Phe Trp Gly Pro Val Ala 
            20                  25                  30          


Asn Trp Gly Leu Pro Ile Ala Ala Ile Asn Asp Met Lys Lys Ser Pro 
        35                  40                  45              


Glu Ile Ile Ser Gly Arg Met Thr Phe Ala Leu Cys Cys Tyr Ser Leu 
    50                  55                  60                  


Thr Phe Met Arg Phe Ala Tyr Lys Val Gln Pro Arg Asn Trp Leu Leu 
65                  70                  75                  80  


Phe Ala Cys His Ala Thr Asn Glu Val Ala Gln Leu Ile Gln Gly Gly 
                85                  90                  95      


Arg Leu Ile Lys His Glu Met Thr Lys Thr Ala Ser Ala 
            100                 105                 


<210>  18
<211>  127
<212>  PRT
<213>  Homo sapiens

<400>  18

Met Ser Ala Ala Gly Ala Arg Gly Leu Arg Ala Thr Tyr His Arg Leu 
1               5                   10                  15      


Leu Asp Lys Val Glu Leu Met Leu Pro Glu Lys Leu Arg Pro Leu Tyr 
            20                  25                  30          


Asn His Pro Ala Gly Pro Arg Thr Val Phe Phe Trp Ala Pro Ile Met 
        35                  40                  45              


Lys Trp Gly Leu Val Cys Ala Gly Leu Ala Asp Met Ala Arg Pro Ala 
    50                  55                  60                  


Glu Lys Leu Ser Thr Ala Gln Ser Ala Val Leu Met Ala Thr Gly Phe 
65                  70                  75                  80  


Ile Trp Ser Arg Tyr Ser Leu Val Ile Ile Pro Lys Asn Trp Ser Leu 
                85                  90                  95      


Phe Ala Val Asn Phe Phe Val Gly Ala Ala Gly Ala Ser Gln Leu Phe 
            100                 105                 110         


Arg Ile Trp Arg Tyr Asn Gln Glu Leu Lys Ala Lys Ala His Lys 
        115                 120                 125         


<210>  19
<211>  30
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  19

Ser Pro Lys Asn Tyr Leu Leu Phe Gly Cys His Leu Ile Asn Glu Thr 
1               5                   10                  15      


Ala Gln Leu Ala Gln Gly Tyr Arg Phe Leu Lys Tyr Thr Tyr 
            20                  25                  30  


<210>  20
<211>  30
<212>  PRT
<213>  Drosophila melanogaster

<400>  20

Gln Pro Arg Asn Trp Leu Leu Phe Ala Cys His Ala Thr Asn Ala Thr 
1               5                   10                  15      


Ala Gln Ser Ile Gln Gly Leu Arg Phe Leu His Tyr Asn Tyr 
            20                  25                  30  


<210>  21
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  21

Gln Pro Arg Asn Trp Leu Leu Phe Ala Cys His Ala Thr Asn Glu Val 
1               5                   10                  15      


Ala Gln Leu Ile Gln Gly Gly Arg Leu Ile Lys His Glu Met 
            20                  25                  30  


<210>  22
<211>  30
<212>  PRT
<213>  Caenorhabditis elegans

<400>  22

Gln Pro Arg Asn Leu Leu Leu Phe Ala Cys His Phe Ala Asn Phe Ser 
1               5                   10                  15      


Ala Gln Gly Ala Gln Leu Gly Arg Phe Val Asn His Asn Tyr 
            20                  25                  30  


<210>  23
<211>  30
<212>  PRT
<213>  Danio rerio

<400>  23

Gln Pro Arg Asn Trp Leu Leu Phe Ala Cys His Phe Thr Asn Glu Gly 
1               5                   10                  15      


Ala Gln Leu Ile Gln Gly Ser Arg Leu Ile Lys Tyr Asn Met 
            20                  25                  30  


<210>  24
<211>  30
<212>  PRT
<213>  Arabidopsis thaliana

<400>  24

Gln Pro Arg Asn Tyr Leu Leu Leu Ala Cys His Ala Ser Asn Glu Thr 
1               5                   10                  15      


Val Gln Leu Tyr Gln Leu Ser Arg Trp Ala Arg Ala Gln Gly 
            20                  25                  30  


<210>  25
<211>  97
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  25

Arg Ser Phe Leu Gln Lys Tyr Ile Asn Lys Glu Thr Leu Lys Tyr Ile 
1               5                   10                  15      


Phe Thr Thr His Phe Trp Gly Pro Val Ser Asn Phe Gly Ile Pro Ile 
            20                  25                  30          


Ala Ala Ile Tyr Asp Leu Lys Lys Asp Pro Thr Leu Ile Ser Gly Pro 
        35                  40                  45              


Met Thr Phe Ala Leu Val Thr Tyr Ser Gly Val Phe Met Lys Tyr Ala 
    50                  55                  60                  


Leu Ser Val Ser Pro Lys Asn Tyr Leu Leu Phe Gly Cys His Leu Ile 
65                  70                  75                  80  


Asn Glu Thr Ala Gln Leu Ala Gln Gly Tyr Arg Phe Leu Lys Tyr Thr 
                85                  90                  95      


Tyr 
    


<210>  26
<211>  97
<212>  PRT
<213>  Drosophila melanogaster

<400>  26

Arg Arg Ala Met Ser Thr Thr Ala Ser Lys Glu Trp Arg Asp Tyr Phe 
1               5                   10                  15      


Met Ser Thr His Phe Trp Gly Pro Val Ala Asn Trp Gly Ile Pro Val 
            20                  25                  30          


Ala Ala Leu Ala Asp Thr Gln Lys Ser Pro Lys Phe Ile Ser Gly Lys 
        35                  40                  45              


Met Thr Leu Ala Leu Thr Leu Tyr Ser Cys Ile Phe Met Arg Phe Ala 
    50                  55                  60                  


Tyr Lys Val Gln Pro Arg Asn Trp Leu Leu Phe Ala Cys His Ala Thr 
65                  70                  75                  80  


Asn Ala Thr Ala Gln Ser Ile Gln Gly Leu Arg Phe Leu His Tyr Asn 
                85                  90                  95      


Tyr 
    


<210>  27
<211>  97
<212>  PRT
<213>  Homo sapiens

<400>  27

Arg Lys Ala Ala Asp Tyr Val Arg Ser Lys Asp Phe Arg Asp Tyr Leu 
1               5                   10                  15      


Met Ser Thr His Phe Trp Gly Pro Val Ala Asn Trp Gly Leu Pro Ile 
            20                  25                  30          


Ala Ala Ile Asn Asp Met Lys Lys Ser Pro Glu Ile Ile Ser Gly Arg 
        35                  40                  45              


Met Thr Phe Ala Leu Cys Cys Tyr Ser Leu Thr Phe Met Arg Phe Ala 
    50                  55                  60                  


Tyr Lys Val Gln Pro Arg Asn Trp Leu Leu Phe Ala Cys His Ala Thr 
65                  70                  75                  80  


Asn Glu Val Ala Gln Leu Ile Gln Gly Gly Arg Leu Ile Lys His Glu 
                85                  90                  95      


Met 
    


<210>  28
<211>  97
<212>  PRT
<213>  Caenorhabditis elegans

<400>  28

Thr Thr Tyr Phe Lys Gln His Ser Thr Ala Glu Trp Lys His Tyr Phe 
1               5                   10                  15      


Leu Ser Thr His Phe Trp Gly Pro Val Ala Asn Trp Gly Leu Pro Leu 
            20                  25                  30          


Ala Ala Leu Gly Asp Leu Lys Lys Asn Pro Asp Met Ile Ser Gly Pro 
        35                  40                  45              


Met Thr Ser Ala Leu Leu Ile Tyr Ser Ser Val Phe Met Arg Phe Ala 
    50                  55                  60                  


Trp His Val Gln Pro Arg Asn Leu Leu Leu Phe Ala Cys His Phe Ala 
65                  70                  75                  80  


Asn Phe Ser Ala Gln Gly Ala Gln Leu Gly Arg Phe Val Asn His Asn 
                85                  90                  95      


Tyr 
    


<210>  29
<211>  97
<212>  PRT
<213>  Danio rerio

<400>  29

Arg Lys Ala Val Asp His Leu Arg Ser Lys Glu Phe Arg Asp Tyr Leu 
1               5                   10                  15      


Met Ser Thr His Phe Trp Gly Pro Val Ala Asn Trp Gly Leu Pro Ile 
            20                  25                  30          


Ala Ala Ile Ser Asp Met Arg Lys Ser Pro Glu Ile Ile Ser Gly Arg 
        35                  40                  45              


Met Thr Phe Ala Leu Thr Cys Tyr Ser Leu Leu Phe Met Arg Phe Ala 
    50                  55                  60                  


Tyr Lys Val Gln Pro Arg Asn Trp Leu Leu Phe Ala Cys His Phe Thr 
65                  70                  75                  80  


Asn Glu Gly Ala Gln Leu Ile Gln Gly Ser Arg Leu Ile Lys Tyr Asn 
                85                  90                  95      


Met 
    


<210>  30
<211>  95
<212>  PRT
<213>  Arabidopsis thaliana

<400>  30

Thr Ser Arg Phe Gln Ala Phe Leu Asn Ser Pro Ile Gly Pro Lys Thr 
1               5                   10                  15      


Thr His Phe Trp Gly Pro Ile Ala Asn Trp Gly Phe Val Ala Ala Gly 
            20                  25                  30          


Leu Val Asp Met Gln Lys Pro Pro Glu Met Ile Ser Gly Asn Met Ser 
        35                  40                  45              


Ser Ala Met Cys Val Tyr Ser Ala Leu Phe Met Arg Phe Ala Trp Met 
    50                  55                  60                  


Val Gln Pro Arg Asn Tyr Leu Leu Leu Ala Cys His Ala Ser Asn Glu 
65                  70                  75                  80  


Thr Val Gln Leu Tyr Gln Leu Ser Arg Trp Ala Arg Ala Gln Gly 
                85                  90                  95  


<210>  31
<211>  95
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  31

Arg Phe Ala Phe Arg Arg Phe Trp Gln Ser Glu Thr Gly Pro Lys Thr 
1               5                   10                  15      


Val His Phe Trp Ala Pro Thr Leu Lys Trp Gly Leu Val Phe Ala Gly 
            20                  25                  30          


Phe Ser Asp Met Lys Arg Pro Val Glu Lys Ile Ser Gly Ala Gln Asn 
        35                  40                  45              


Leu Ser Leu Leu Ser Thr Ala Leu Ile Trp Thr Arg Trp Ser Phe Val 
    50                  55                  60                  


Ile Lys Pro Arg Asn Ile Leu Leu Ala Ser Val Asn Ser Phe Leu Cys 
65                  70                  75                  80  


Leu Thr Ala Gly Tyr Gln Leu Gly Arg Ile Ala Asn Tyr Arg Ile 
                85                  90                  95  


<210>  32
<211>  95
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  32

Asn Phe Ala Phe Arg Arg Phe Trp Asn Ser Glu Thr Gly Pro Lys Thr 
1               5                   10                  15      


Val His Phe Trp Ala Pro Thr Leu Lys Trp Gly Leu Val Phe Ala Gly 
            20                  25                  30          


Leu Asn Asp Ile Lys Arg Pro Val Glu Lys Val Ser Gly Ala Gln Asn 
        35                  40                  45              


Leu Ser Leu Leu Ala Thr Ala Leu Ile Trp Thr Arg Trp Ser Phe Val 
    50                  55                  60                  


Ile Lys Pro Lys Asn Tyr Leu Leu Ala Ser Val Asn Phe Phe Leu Gly 
65                  70                  75                  80  


Cys Thr Ala Gly Tyr His Leu Thr Arg Ile Ala Asn Phe Arg Ile 
                85                  90                  95  


<210>  33
<211>  95
<212>  PRT
<213>  Drosophila melanogaster

<400>  33

Pro Pro Lys Met Arg Pro Leu Trp Met His Pro Ala Gly Pro Lys Thr 
1               5                   10                  15      


Ile Phe Phe Trp Ala Pro Ile Val Lys Trp Ser Leu Val Ile Ala Gly 
            20                  25                  30          


Leu Ser Asp Leu Thr Arg Pro Ala Asp Lys Ile Ser Pro Asn Gly Cys 
        35                  40                  45              


Leu Ala Leu Gly Ala Thr Asn Leu Ile Trp Thr Arg Tyr Ser Leu Val 
    50                  55                  60                  


Ile Ile Pro Lys Asn Tyr Ser Leu Phe Ala Val Asn Leu Phe Val Ser 
65                  70                  75                  80  


Leu Thr Gln Leu Phe Gln Leu Gly Arg Tyr Tyr Asn Tyr Gln Trp 
                85                  90                  95  


<210>  34
<211>  95
<212>  PRT
<213>  Homo sapiens

<400>  34

Pro Glu Lys Leu Arg Pro Leu Tyr Asn His Pro Ala Gly Pro Arg Thr 
1               5                   10                  15      


Val Phe Phe Trp Ala Pro Ile Met Lys Trp Gly Leu Val Cys Ala Gly 
            20                  25                  30          


Leu Ala Asp Met Ala Arg Pro Ala Glu Lys Leu Ser Thr Ala Gln Ser 
        35                  40                  45              


Ala Val Leu Met Ala Thr Gly Phe Ile Trp Ser Arg Tyr Ser Leu Val 
    50                  55                  60                  


Ile Ile Pro Lys Asn Trp Ser Leu Phe Ala Val Asn Phe Phe Val Gly 
65                  70                  75                  80  


Ala Ala Gly Ala Ser Gln Leu Phe Arg Ile Trp Arg Tyr Asn Gln 
                85                  90                  95  


<210>  35
<211>  95
<212>  PRT
<213>  Caenorhabditis elegans

<400>  35

Pro Ala Phe Ala Lys Pro Ala Trp Asn His Ala Ala Gly Pro Lys Thr 
1               5                   10                  15      


Val Phe Phe Trp Ala Pro Thr Ile Lys Trp Thr Leu Ile Gly Ala Gly 
            20                  25                  30          


Leu Ala Asp Leu Ala Arg Pro Ala Asp Lys Leu Ser Leu Tyr Gln Asn 
        35                  40                  45              


Ser Ala Leu Phe Ala Thr Gly Ala Ile Trp Thr Arg Tyr Cys Leu Val 
    50                  55                  60                  


Ile Thr Pro Ile Asn Tyr Tyr Leu Ser Ser Val Asn Phe Phe Val Met 
65                  70                  75                  80  


Cys Thr Gly Leu Ala Gln Leu Cys Arg Ile Ala His Tyr Arg Tyr 
                85                  90                  95  


<210>  36
<211>  95
<212>  PRT
<213>  Danio rerio

<400>  36

Pro Ala Lys Leu Arg Pro Phe Tyr Asn His Pro Ala Gly Pro Lys Thr 
1               5                   10                  15      


Val Phe Phe Trp Ala Pro Met Phe Lys Trp Gly Leu Val Leu Ala Gly 
            20                  25                  30          


Leu Ala Asp Met Ala Arg Pro Ala Glu Lys Leu Ser Thr Ser Gln Ser 
        35                  40                  45              


Ala Val Leu Thr Ala Thr Gly Leu Ile Trp Ser Arg Tyr Ser Leu Val 
    50                  55                  60                  


Ile Ile Pro Lys Asn Trp Asn Leu Phe Ala Val Asn Phe Phe Val Gly 
65                  70                  75                  80  


Ser Ala Gly Gly Ser Gln Leu Tyr Arg Ile Trp Met His Asn Arg 
                85                  90                  95  


<210>  37
<211>  95
<212>  PRT
<213>  Arabidopsis thaliana

<400>  37

Thr Ser Lys Leu Gln Ala Ile Trp Asn His Pro Ala Gly Pro Lys Thr 
1               5                   10                  15      


Ile His Phe Trp Ala Pro Thr Phe Lys Trp Gly Ile Ser Ile Ala Asn 
            20                  25                  30          


Ile Ala Asp Phe Ala Lys Pro Pro Glu Lys Leu Ser Tyr Pro Gln Gln 
        35                  40                  45              


Ile Ala Val Thr Cys Thr Gly Val Ile Trp Ser Arg Tyr Ser Met Val 
    50                  55                  60                  


Ile Asn Pro Lys Asn Trp Asn Leu Phe Ser Val Asn Val Ala Met Ala 
65                  70                  75                  80  


Gly Thr Gly Ile Tyr Gln Leu Ala Arg Lys Ile Lys His Asp Tyr 
                85                  90                  95  


<210>  38
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; forward primer for eGFP

<400>  38
ctcgaggtga gcaagggcga gg                                                22


<210>  39
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; reverse primer for eGFP

<400>  39
ggtaccttac ttgtacagct cgtccatg                                          28


<210>  40
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; forward primer for dMPC1

<400>  40
cggaattcaa ctttcggagt gacaacacg                                         29


<210>  41
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; reverse primer for dMPC1

<400>  41
cgctcgaggg ctgccgcctg ctgctcctta gac                                    33


<210>  42
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; MPC1 siRNA

<400>  42
ucaacuacga gaugaguaat t                                                 21


<210>  43
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; MPC1 si RNA

<400>  43
gggaaaacac agaaugcuat t                                                 21


<210>  44
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; MPC1 siRNA

<400>  44
ccauguaaca aacgaaguat t                                                 21


<210>  45
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; MPC2 siRNA

<400>  45
ccgauaaggu gaugcuaaat t                                                 21


<210>  46
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; MPC2 siRNA

<400>  46
uggauaaagu ggaguuguut t                                                 21


<210>  47
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; MPC2 siRNA

<400>  47
accaagaacu caaaucuaat t                                                 21


