                         SEQUENCE LISTING

<110>  Shaheen, Hussam H
       Zha, Dongxing
 
<120>  SURFACE ANCHORED LIGHT CHAIN BAIT ANTIBODY DISPLAY SYSTEM

<130>  23234GFIBIO

<150>  61/645,763
<151>  2012-05-11

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  322
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  1

Val Asp Gln Phe Ser Asn Ser Thr Ser Ala Ser Ser Thr Asp Val Thr 
1               5                   10                  15      


Ser Ser Ser Ser Ile Ser Thr Ser Ser Gly Ser Val Thr Ile Thr Ser 
            20                  25                  30          


Ser Glu Ala Pro Glu Ser Asp Asn Gly Thr Ser Thr Ala Ala Pro Thr 
        35                  40                  45              


Glu Thr Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly Thr 
    50                  55                  60                  


Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly Thr Ser Thr 
65                  70                  75                  80  


Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr Ala Leu Pro 
                85                  90                  95      


Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala 
            100                 105                 110         


Pro Thr Thr Gly Leu Pro Thr Asn Gly Thr Thr Ser Ala Phe Pro Pro 
        115                 120                 125             


Thr Thr Ser Leu Pro Pro Ser Asn Thr Thr Thr Thr Pro Pro Tyr Asn 
    130                 135                 140                 


Pro Ser Thr Asp Tyr Thr Thr Asp Tyr Thr Val Val Thr Glu Tyr Thr 
145                 150                 155                 160 


Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr 
                165                 170                 175     


Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr 
            180                 185                 190         


Ile Glu Lys Pro Thr Thr Thr Ser Thr Thr Glu Tyr Thr Val Val Thr 
        195                 200                 205             


Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly 
    210                 215                 220                 


Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys 
225                 230                 235                 240 


Pro Cys Thr Ile Glu Lys Ser Glu Ala Pro Glu Ser Ser Val Pro Val 
                245                 250                 255     


Thr Glu Ser Lys Gly Thr Thr Thr Lys Glu Thr Gly Val Thr Thr Lys 
            260                 265                 270         


Gln Thr Thr Ala Asn Pro Ser Leu Thr Val Ser Thr Val Val Pro Val 
        275                 280                 285             


Ser Ser Ser Ala Ser Ser His Ser Val Val Ile Asn Ser Asn Gly Ala 
    290                 295                 300                 


Asn Val Val Val Pro Gly Ala Leu Gly Leu Ala Gly Val Ala Met Leu 
305                 310                 315                 320 


Phe Leu 
        


<210>  2
<211>  960
<212>  DNA
<213>  Pichia pastoris

<400>  2
caattctcta actctacttc cgcttcctct actgacgtta cttcctcctc ctctatttct       60

acttcctccg gttccgttac tattacttcc tctgaggctc cagaatctga caacggtact      120

tctactgctg ctccaactga aacttctact gaggctccta ctactgctat tccaactaac      180

ggaacttcca cagaggctcc aacaacagct atccctacaa acggtacatc cactgaagct      240

cctactgaca ctactacaga agctccaact actgctttgc ctactaatgg tacatcaaca      300

gaggctccta cagatacaac aactgaagct ccaacaactg gattgccaac aaacggtact      360

acttctgctt tcccaccaac tacttccttg ccaccatcca acactactac tactccacca      420

tacaacccat ccactgacta cactactgac tacacagttg ttactgagta cactacttac      480

tgtccagagc caactacttt cacaacaaac ggaaagactt acactgttac tgagcctact      540

actttgacta tcactgactg tccatgtact atcgagaagc caactactac ttccactaca      600

gagtatactg ttgttacaga atacacaaca tattgtcctg agccaacaac attcactact      660

aatggaaaaa catacacagt tacagaacca actacattga caattacaga ttgtccttgt      720

acaattgaga agtccgaggc tcctgaatct tctgttccag ttactgaatc caagggtact      780

actactaaag aaactggtgt tactactaag cagactactg ctaacccatc cttgactgtt      840

tccactgttg ttccagtttc ttcctctgct tcttcccact ccgttgttat caactccaac      900

ggtgctaacg ttgttgttcc tggtgctttg ggattggctg gtgttgctat gttgttcttg      960


<210>  3
<211>  19
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  3

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala 
            


<210>  4
<211>  57
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  4
atgagattcc catccatctt cactgctgtt ttgttcgctg cttcctctgc tttggct          57


<210>  5
<211>  551
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human light chain immunoglobulin domain fused to a Pichia 
       pastoris Sed1p polypeptide

<400>  5

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Val Gly Thr Asn 
            20                  25                  30          


Val Val Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Ala Leu Ile 
        35                  40                  45              


His Ser Ala Ser Tyr Arg Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Lys Thr Tyr Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    210                 215                 220                 


Gly Gly Gly Gly Ser Val Asp Gln Phe Ser Asn Ser Thr Ser Ala Ser 
225                 230                 235                 240 


Ser Thr Asp Val Thr Ser Ser Ser Ser Ile Ser Thr Ser Ser Gly Ser 
                245                 250                 255     


Val Thr Ile Thr Ser Ser Glu Ala Pro Glu Ser Asp Asn Gly Thr Ser 
            260                 265                 270         


Thr Ala Ala Pro Thr Glu Thr Ser Thr Glu Ala Pro Thr Thr Ala Ile 
        275                 280                 285             


Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr 
    290                 295                 300                 


Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala Pro 
305                 310                 315                 320 


Thr Thr Ala Leu Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp 
                325                 330                 335     


Thr Thr Thr Glu Ala Pro Thr Thr Gly Leu Pro Thr Asn Gly Thr Thr 
            340                 345                 350         


Ser Ala Phe Pro Pro Thr Thr Ser Leu Pro Pro Ser Asn Thr Thr Thr 
        355                 360                 365             


Thr Pro Pro Tyr Asn Pro Ser Thr Asp Tyr Thr Thr Asp Tyr Thr Val 
    370                 375                 380                 


Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr 
385                 390                 395                 400 


Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr 
                405                 410                 415     


Asp Cys Pro Cys Thr Ile Glu Lys Pro Thr Thr Thr Ser Thr Thr Glu 
            420                 425                 430         


Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr 
        435                 440                 445             


Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu 
    450                 455                 460                 


Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Ser Glu Ala Pro Glu 
465                 470                 475                 480 


Ser Ser Val Pro Val Thr Glu Ser Lys Gly Thr Thr Thr Lys Glu Thr 
                485                 490                 495     


Gly Val Thr Thr Lys Gln Thr Thr Ala Asn Pro Ser Leu Thr Val Ser 
            500                 505                 510         


Thr Val Val Pro Val Ser Ser Ser Ala Ser Ser His Ser Val Val Ile 
        515                 520                 525             


Asn Ser Asn Gly Ala Asn Val Val Val Pro Gly Ala Leu Gly Leu Ala 
    530                 535                 540                 


Gly Val Ala Met Leu Phe Leu 
545                 550     


<210>  6
<211>  1647
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human light chain immunoglobulin domain fused to a Pichia 
       pastoris Sed1p polypeptide

<400>  6
gacattcaaa tgactcagtc cccatcttcc ttgtctgctt ccgttggtga cagagttact       60

atcacttgta aggcttccca gaacgttgga actaacgttg tttggtatca gcagaagcca      120

ggtaaggctc caaaggcttt gattcactcc gcttcataca gatactccgg tgttccatcc      180

agattctctg gttctggttc cggtactgac tttactttga ctatctcctc attgcagcca      240

gaggacttcg ctacttacta ctgtcagcag tacaagactt acccatacac tttcggtcag      300

ggtaccaagg ttgagatcaa gagaactgtt gctgctccat ccgttttcat tttcccacca      360

tccgacgaac agttgaagtc tggtacagct tccgttgttt gtttgttgaa caacttctac      420

ccaagagagg ctaaggttca gtggaaggtt gacaacgctt tgcaatccgg taactcccaa      480

gaatccgtta ctgagcaaga ctctaaggac tccacttact ccttgtcctc cactttgact      540

ttgtccaagg ctgattacga gaagcacaag gtttacgctt gtgaggttac acatcagggt      600

ttgtcctccc cagttactaa gtccttcaac agaggagagt gtggtggtgg tggttccggt      660

ggtggtggtt ctggtggtgg tggttctcaa ttctctaact ctacttccgc ttcctctact      720

gacgttactt cctcctcctc tatttctact tcctccggtt ccgttactat tacttcctct      780

gaggctccag aatctgacaa cggtacttct actgctgctc caactgaaac ttctactgag      840

gctcctacta ctgctattcc aactaacgga acttccacag aggctccaac aacagctatc      900

cctacaaacg gtacatccac tgaagctcct actgacacta ctacagaagc tccaactact      960

gctttgccta ctaatggtac atcaacagag gctcctacag atacaacaac tgaagctcca     1020

acaactggat tgccaacaaa cggtactact tctgctttcc caccaactac ttccttgcca     1080

ccatccaaca ctactactac tccaccatac aacccatcca ctgactacac tactgactac     1140

acagttgtta ctgagtacac tacttactgt ccagagccaa ctactttcac aacaaacgga     1200

aagacttaca ctgttactga gcctactact ttgactatca ctgactgtcc atgtactatc     1260

gagaagccaa ctactacttc cactacagag tatactgttg ttacagaata cacaacatat     1320

tgtcctgagc caacaacatt cactactaat ggaaaaacat acacagttac agaaccaact     1380

acattgacaa ttacagattg tccttgtaca attgagaagt ccgaggctcc tgaatcttct     1440

gttccagtta ctgaatccaa gggtactact actaaagaaa ctggtgttac tactaagcag     1500

actactgcta acccatcctt gactgtttcc actgttgttc cagtttcttc ctctgcttct     1560

tcccactccg ttgttatcaa ctccaacggt gctaacgttg ttgttcctgg tgctttggga     1620

ttggctggtg ttgctatgtt gttcttg                                         1647


<210>  7
<211>  9180
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGLY11714

<400>  7
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gagatctaac atccaaagac      420

gaaaggttga atgaaacctt tttgccatcc gacatccaca ggtccattct cacacataag      480

tgccaaacgc aacaggaggg gatacactag cagcagaccg ttgcaaacgc aggacctcca      540

ctcctcttct cctcaacacc cacttttgcc atcgaaaaac cagcccagtt attgggcttg      600

attggagctc gctcattcca attccttcta ttaggctact aacaccatga ctttattagc      660

ctgtctatcc tggcccccct ggcgaggttc atgtttgttt atttccgaat gcaacaagct      720

ccgcattaca cccgaacatc actccagatg agggctttct gagtgtgggg tcaaatagtt      780

tcatgttccc caaatggccc aaaactgaca gtttaaacgc tgtcttggaa cctaatatga      840

caaaagcgtg atctcatcca agatgaacta agtttggttc gttgaaatgc taacggccag      900

ttggtcaaaa agaaacttcc aaaagtcggc ataccgtttg tcttgtttgg tattgattga      960

cgaatgctca aaaataatct cattaatgct tagcgcagtc tctctatcgc ttctgaaccc     1020

cggtgcacct gtgccgaaac gcaaatgggg aaacacccgc tttttggatg attatgcatt     1080

gtctccacat tgtatgcttc caagattctg gtgggaatac tgctgatagc ctaacgttca     1140

tgatcaaaat ttaactgttc taacccctac ttgacagcaa tatataaaca gaaggaagct     1200

gccctgtctt aaaccttttt ttttatcatc attattagct tactttcata attgcgactg     1260

gttccaattg acaagctttt gattttaacg acttttaacg acaacttgag aagatcaaaa     1320

aacaactaat tattcgaaac ggaattcacg atgagattcc catccatctt cactgctgtt     1380

ttgttcgctg cttcctctgc tttggctgac attcaaatga ctcagtcccc atcttccttg     1440

tctgcttccg ttggtgacag agttactatc acttgtaagg cttcccagaa cgttggaact     1500

aacgttgttt ggtatcagca gaagccaggt aaggctccaa aggctttgat tcactccgct     1560

tcatacagat actccggtgt tccatccaga ttctctggtt ctggttccgg tactgacttt     1620

actttgacta tctcctcatt gcagccagag gacttcgcta cttactactg tcagcagtac     1680

aagacttacc catacacttt cggtcagggt accaaggttg agatcaagag aactgttgct     1740

gctccatccg ttttcatttt cccaccatcc gacgaacagt tgaagtctgg tacagcttcc     1800

gttgtttgtt tgttgaacaa cttctaccca agagaggcta aggttcagtg gaaggttgac     1860

aacgctttgc aatccggtaa ctcccaagaa tccgttactg agcaagactc taaggactcc     1920

acttactcct tgtcctccac tttgactttg tccaaggctg attacgagaa gcacaaggtt     1980

tacgcttgtg aggttacaca tcagggtttg tcctccccag ttactaagtc cttcaacaga     2040

ggagagtgtg gtggtggtgg ttccggtggt ggtggttctg gtggtggtgg ttctgtcgac     2100

caattctcta actctacttc cgcttcctct actgacgtta cttcctcctc ctctatttct     2160

acttcctccg gttccgttac tattacttcc tctgaggctc cagaatctga caacggtact     2220

tctactgctg ctccaactga aacttctact gaggctccta ctactgctat tccaactaac     2280

ggaacttcca cagaggctcc aacaacagct atccctacaa acggtacatc cactgaagct     2340

cctactgaca ctactacaga agctccaact actgctttgc ctactaatgg tacatcaaca     2400

gaggctccta cagatacaac aactgaagct ccaacaactg gattgccaac aaacggtact     2460

acttctgctt tcccaccaac tacttccttg ccaccatcca acactactac tactccacca     2520

tacaacccat ccactgacta cactactgac tacacagttg ttactgagta cactacttac     2580

tgtccagagc caactacttt cacaacaaac ggaaagactt acactgttac tgagcctact     2640

actttgacta tcactgactg tccatgtact atcgagaagc caactactac ttccactaca     2700

gagtatactg ttgttacaga atacacaaca tattgtcctg agccaacaac attcactact     2760

aatggaaaaa catacacagt tacagaacca actacattga caattacaga ttgtccttgt     2820

acaattgaga agtccgaggc tcctgaatct tctgttccag ttactgaatc caagggtact     2880

actactaaag aaactggtgt tactactaag cagactactg ctaacccatc cttgactgtt     2940

tccactgttg ttccagtttc ttcctctgct tcttcccact ccgttgttat caactccaac     3000

ggtgctaacg ttgttgttcc tggtgctttg ggattggctg gtgttgctat gttgttcttg     3060

taatagggcc ggccatttaa atacaggccc cttttccttt gtcgatatca tgtaattagt     3120

tatgtcacgc ttacattcac gccctcctcc cacatccgct ctaaccgaaa aggaaggagt     3180

tagacaacct gaagtctagg tccctattta ttttttttaa tagttatgtt agtattaaga     3240

acgttattta tatttcaaat ttttcttttt tttctgtaca aacgcgtgta cgcatgtaac     3300

attatactga aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt aatttgcaag     3360

ctggatccgc ggccgcttac gcgccgttct tcgcttggtc ttgtatctcc ttacactgta     3420

tcttcccatt tgcgtttagg tggttatcaa aaactaaaag gaaaaatttc agatgtttat     3480

ctctaaggtt ttttcttttt acagtataac acgtgatgcg tcacgtggta ctagattacg     3540

taagttattt tggtccggtg ggtaagtggg taagaataga aagcatgaag gtttacaaaa     3600

acgcagtcac gaattattgc tacttcgagc ttggaaccac cccaaagatt atattgtact     3660

gatgcactac cttctcgatt ttgctcctcc aagaacctac gaaaaacatt tcttgagcct     3720

tttcaaccta gactacacat caagttattt aaggtatgtt ccgttaacat gtaagaaaag     3780

gagaggatag atcgtttatg gggtacgtcg cctgattcaa gcgtgaccat tcgaagaata     3840

ggccttcgaa agctgaataa agcaaatgtc agttgcgatt ggtatgctga caaattagca     3900

taaaaagcaa tagactttct aaccacctgt ttttttcctt ttactttatt tatattttgc     3960

caccgtacta acaagttcag acaaattaat taacaccatg tcagaagatc aaaaaagtga     4020

aaattccgta ccttctaagg ttaatatggt gaatcgcacc gatatactga ctacgatcaa     4080

gtcattgtca tggcttgact tgatgttgcc atttactata attctctcca taatcattgc     4140

agtaataatt tctgtctatg tgccttcttc ccgtcacact tttgacgctg aaggtcatcc     4200

caatctaatg ggagtgtcca ttcctttgac tgttggtatg attgtaatga tgattccccc     4260

gatctgcaaa gtttcctggg agtctattca caagtacttc tacaggagct atataaggaa     4320

gcaactagcc ctctcgttat ttttgaattg ggtcatcggt cctttgttga tgacagcatt     4380

ggcgtggatg gcgctattcg attataagga ataccgtcaa ggcattatta tgatcggagt     4440

agctagatgc attgccatgg tgctaatttg gaatcagatt gctggaggag acaatgatct     4500

ctgcgtcgtg cttgttatta caaactcgct tttacagatg gtattatatg caccattgca     4560

gatattttac tgttatgtta tttctcatga ccacctgaat acttcaaata gggtattatt     4620

cgaagaggtt gcaaagtctg tcggagtttt tctcggcata ccactgggaa ttggcattat     4680

catacgtttg ggaagtctta ccatagctgg taaaagtaat tatgaaaaat acattttgag     4740

atttatttct ccatgggcaa tgatcggatt tcattacact ttatttgtta tttttattag     4800

tagaggttat caatttatcc acgaaattgg ttctgcaata ttgtgctttg tcccattggt     4860

gctttacttc tttattgcat ggtttttgac cttcgcatta atgaggtact tatcaatatc     4920

taggagtgat acacaaagag aatgtagctg tgaccaagaa ctacttttaa agagggtctg     4980

gggaagaaag tcttgtgaag ctagcttttc tattacgatg acgcaatgtt tcactatggc     5040

ttcaaataat tttgaactat ccctggcaat tgctatttcc ttatatggta acaatagcaa     5100

gcaagcaata gctgcaacat ttgggccgtt gctagaagtt ccaattttat tgattttggc     5160

aatagtcgcg agaatcctta aaccatatta tatatggaac aatagaaatt aattaacagg     5220

ccccttttcc tttgtcgata tcatgtaatt agttatgtca cgcttacatt cacgccctcc     5280

tcccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat     5340

ttattttttt taatagttat gttagtatta agaacgttat ttatatttca aatttttctt     5400

ttttttctgt acaaacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag     5460

gttttgggac gctcgaaggc tttaatttgc aagctgcggc ctaaggcgcg ccaggccata     5520

atggcccaaa tgcaagagga cattagaaat gtgtttggta agaacatgaa gccggaggca     5580

tacaaacgat tcacagattt gaaggaggaa aacaaactgc atccaccgga agtgccagca     5640

gccgtgtatg ccaaccttgc tctcaaaggc attcctacgg atctgagtgg gaaatatctg     5700

agattcacag acccactatt ggaacagtac caaacctagt ttggccgatc catgattatg     5760

taatgcatat agtttttgtc gatgctcacc cgtttcgagt ctgtctcgta tcgtcttacg     5820

tataagttca agcatgttta ccaggtctgt tagaaactcc tttgtgaggg caggacctat     5880

tcgtctcggt cccgttgttt ctaagagact gtacagccaa gcgcagaatg gtggcattaa     5940

ccataagagg attctgatcg gacttggtct attggctatt ggaaccaccc tttacgggac     6000

aaccaaccct accaagactc ctattgcatt tgtggaacca gccacggaaa gagcgtttaa     6060

ggacggagac gtctctgtga tttttgttct cggaggtcca ggagctggaa aaggtaccca     6120

atgtgccaaa ctagtgagta attacggatt tgttcacctg tcagctggag acttgttacg     6180

tgcagaacag aagagggagg ggtctaagta tggagagatg atttcccagt atatcagaga     6240

tggactgata gtacctcaag aggtcaccat tgcgctcttg gagcaggcca tgaaggaaaa     6300

cttcgagaaa gggaagacac ggttcttgat tgatggattc cctcgtaaga tggaccaggc     6360

caaaactttt gaggaaaaag tcgcaaagtc caaggtgaca cttttctttg attgtcccga     6420

atcagtgctc cttgagagat tacttaaaag aggacagaca agcggaagag aggatgataa     6480

tgcggagagt atcaaaaaaa gattcaaaac attcgtggaa acttcgatgc ctgtggtgga     6540

ctatttcggg aagcaaggac gcgttttgaa ggtatcttgt gaccaccctg tggatcaagt     6600

gtattcacag gttgtgtcgg tgctaaaaga gaaggggatc tttgccgata acgagacgga     6660

gaataaataa acattgtaat aagatttaga ctgtgaatgt tctatgtaat atttttcgag     6720

atactgtatc tatctggtgt accgtatcac tctggacttg caaactcatt gattacttgt     6780

gcaatgggca agaaggatag ctctagaaag aagaagaaaa aggagccgcc tgaagagctg     6840

gatctttccg aggttgttcc aacttttggt tatgaggaat ttcatgttga gcaagaggag     6900

aatccggtcg atcaagacga acttgacggc cataatggcc tagcttggcg taatcatggt     6960

catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg     7020

gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt     7080

tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg     7140

gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg     7200

actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa     7260

tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc     7320

aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc     7380

ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat     7440

aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc     7500

cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct     7560

cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg     7620

aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc     7680

cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga     7740

ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa     7800

ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta     7860

gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc     7920

agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg     7980

acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga     8040

tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg     8100

agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct     8160

gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg     8220

agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc     8280

cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa     8340

ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc     8400

cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt     8460

cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc     8520

ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt     8580

tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc     8640

catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt     8700

gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata     8760

gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga     8820

tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag     8880

catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa     8940

aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt     9000

attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga     9060

aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag     9120

aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc     9180


<210>  8
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized mouse Ig

<400>  8

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Val Gly Thr Asn 
            20                  25                  30          


Val Val Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Ala Leu Ile 
        35                  40                  45              


His Ser Ala Ser Tyr Arg Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Lys Thr Tyr Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  9
<211>  570
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of alpha mating factor-antipPCSK9 Lc-(GGGS) 
       linker-S. cerevisiae Sed1p

<400>  9

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala 
            20                  25                  30          


Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Val 
        35                  40                  45              


Gly Thr Asn Val Val Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys 
    50                  55                  60                  


Ala Leu Ile His Ser Ala Ser Tyr Arg Tyr Ser Gly Val Pro Ser Arg 
65                  70                  75                  80  


Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser 
                85                  90                  95      


Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Lys Thr 
            100                 105                 110         


Tyr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr 
        115                 120                 125             


Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu 
    130                 135                 140                 


Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro 
145                 150                 155                 160 


Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly 
                165                 170                 175     


Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr 
            180                 185                 190         


Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His 
        195                 200                 205             


Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val 
    210                 215                 220                 


Thr Lys Ser Phe Asn Arg Gly Glu Cys Gly Gly Gly Gly Ser Gly Gly 
225                 230                 235                 240 


Gly Gly Ser Gly Gly Gly Gly Ser Val Asp Gln Phe Ser Asn Ser Thr 
                245                 250                 255     


Ser Ala Ser Ser Thr Asp Val Thr Ser Ser Ser Ser Ile Ser Thr Ser 
            260                 265                 270         


Ser Gly Ser Val Thr Ile Thr Ser Ser Glu Ala Pro Glu Ser Asp Asn 
        275                 280                 285             


Gly Thr Ser Thr Ala Ala Pro Thr Glu Thr Ser Thr Glu Ala Pro Thr 
    290                 295                 300                 


Thr Ala Ile Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Thr Ala 
305                 310                 315                 320 


Ile Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr 
                325                 330                 335     


Glu Ala Pro Thr Thr Ala Leu Pro Thr Asn Gly Thr Ser Thr Glu Ala 
            340                 345                 350         


Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr Gly Leu Pro Thr Asn 
        355                 360                 365             


Gly Thr Thr Ser Ala Phe Pro Pro Thr Thr Ser Leu Pro Pro Ser Asn 
    370                 375                 380                 


Thr Thr Thr Thr Pro Pro Tyr Asn Pro Ser Thr Asp Tyr Thr Thr Asp 
385                 390                 395                 400 


Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr 
                405                 410                 415     


Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu 
            420                 425                 430         


Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Pro Thr Thr Thr Ser 
        435                 440                 445             


Thr Thr Glu Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu 
    450                 455                 460                 


Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro 
465                 470                 475                 480 


Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Ser Glu 
                485                 490                 495     


Ala Pro Glu Ser Ser Val Pro Val Thr Glu Ser Lys Gly Thr Thr Thr 
            500                 505                 510         


Lys Glu Thr Gly Val Thr Thr Lys Gln Thr Thr Ala Asn Pro Ser Leu 
        515                 520                 525             


Thr Val Ser Thr Val Val Pro Val Ser Ser Ser Ala Ser Ser His Ser 
    530                 535                 540                 


Val Val Ile Asn Ser Asn Gly Ala Asn Val Val Val Pro Gly Ala Leu 
545                 550                 555                 560 


Gly Leu Ala Gly Val Ala Met Leu Phe Leu 
                565                 570 


<210>  10
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  10

Gly Gly Gly Ser 
1               


