                         SEQUENCE LISTING

<110>  VIB VZW
       UNIVERSITEIT GENT
 
<120>  TRITERPENOID SAPOGENIN PRODUCTION IN PLANT AND MICROBIAL CULTURES

<130>  AlGo/CYC/415

<150>  US 61/645,998
<151>  2012-05-11

<150>  US 61/732,817
<151>  2012-12-03

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  1437
<212>  DNA
<213>  Bupleurum falcatum

<400>  1
atggaacttt ctatcactct gatgcttatt ttctcaacaa ccatcttctt tatatttcgt       60

aatgtgtaca accatctcat ctctaaacac aaaaactatc cccctggaag tatgggcttg      120

ccttacattg gcgaaacact tagtttcgcg agatacatca ccaaaggagt ccctgaaaaa      180

ttcgtaatag aaagacaaaa gaaatattca acaacaatat ttaagacctc cttgttcgga      240

gaaaacatgg tggtgttggg cagtgcagag ggcaacaaat ttatttttgg aagcgaggag      300

aagtatttac gagtgtggtt tccaagttct gtggacaaag tgttcaaaaa atctcataag      360

agaacgtcgc aggaagaagc tattaggttg cgcaaaaaca tggtgccatt tctcaaagca      420

gatttgttga gaagttatgt accaataatg gacacattta tgaaacaaca tgtgaactcg      480

cattggaatt gcgagacctt gaaggcttgt cctgtgatca aggattttac gtttacttta      540

gcttgtaaac ttttttttag tgtagacaat cctttggagc tagagaagtt aatcaagcta      600

tttgtgaata tagtgaatgg cctccttacg gtccctattg atctcccggg gacaaaattt      660

agaggagtta taaagagtgt caagactatt cgccatgcgc ttaaagtgtt gatcaggcaa      720

cgaaaggtgg atattagaga gaaaagagcc acacctacgc aagatatatt gtcgataatg      780

ctggcacagg ctgaggacga gaactatgaa atgaatgatg aagatgtggc caatgacttt      840

cttgcagttt tgcttgctag ttatgattct gccaatacta cactcaccat gattatgaaa      900

tatcttgctg aatatcccga aatgtatgat cgagttttca gagaacaaat ggaggtggca      960

aagacgaaag gaaaagatga attactcaac ttggacgact tgcaaaagat gaattatact     1020

tggaatgtag cttgtgaagt actgagaatt gcaacaccaa cgttcggagc attcagagag     1080

gttattgcag attgtacata cgaagggtac accataccaa aaggctggaa gctatattat     1140

gccccgcgtt ttacccatgg aagtgcaaaa tactttcaag atccagagaa atttgatcca     1200

tcgcgatttg aaggtgatgg tgcgcctcct tatacattcg ttccattcgg aggagggctc     1260

cggatgtgcc ctggatacaa gtatgcaaag attatagtac tagtgttcat gcacaatata     1320

gttacaaagt tcaaatggga gaaagttaac cctaatgaga aaatgacagt aggaatcgta     1380

tcagcgccaa gtcaaggact tccactgcgt ctccatcccc acaaatctcc atcttaa        1437


<210>  2
<211>  1428
<212>  DNA
<213>  Maesa lanceolata

<400>  2
atgtgggtag tgggattaat tggtgtggct gtggtaacaa tattgataac tcagtatgta       60

tacaaatgga gaaatccaaa gactgtgggt gttctgccac ctggttcaat gggtctgcct      120

ttgatcgggg agactcttca acttctcagc cgtaatccat ccttggatct tcatcctttc      180

atcaagagca gaatccaaag atatgggcag atattcgcga ccaatatcgt aggtcgaccc      240

ataatagtaa ccgctgatcc gcagctcaat aattaccttt tccaacaaga aggaagagca      300

gtagaactgt ggtacttgga cagctttcaa aagctattta acttagaagg tgcaaacagg      360

ccgaacgcag ttggtcacat tcacaagtac gttagaagtg tatacttgag tctctttggc      420

gtcgagagcc ttaaaacaaa gttgcttgcc gatattgaga aaacagtccg caaaaatctt      480

attggtggga caaccaaagg cacctttgat gcaaaacatg cttctgccaa tatggttgct      540

gtttttgctg caaaatactt gttcggacat gattacgaga aatcgaaaga agatgtaggc      600

agcataatcg acaacttcgt acaaggtctt ctcgcattcc cattgaatgt tcccggtaca      660

aagttccaca aatgtatgaa ggacaagaaa aggctggaat caatgatcac taacaagcta      720

aaggagagaa tagctgatcc gaacagcgga caaggggatt tccttgatca agcagtgaaa      780

gacttgaata gcgaattctt cataacagag acttttatcg tttcggtgac gatgggagct      840

ttatttgcga cggttgaatc ggtttcgaca gcaattggac tagctttcaa gttttttgca      900

gagcacccct gggttttgga tgacctcaag gctgagcatg aggctgtcct tagcaaaaga      960

gaggatagaa attcacctct cacgtgggac gaatatagat cgatgacaca cacgatgcac     1020

tttatcaatg aagtcgtccg tttgggaaat gtttttcctg gaattttgag gaaagcactg     1080

aaagatattc catataatgg ttatacaatt ccgtccggtt ggaccattat gattgtgacc     1140

tctacccttg cgatgaaccc tgagatattc aaggatcctc ttgcattcaa tccgaaacgt     1200

tggcgggata ttgatcccga aactcaaact aaaaacttta tgcctttcgg tggtgggacg     1260

agacaatgcg caggtgcaga gctagccaag gcattctttg ctaccttcct ccatgtttta     1320

atcagcgaat atagctggaa gaaagtgaag ggaggaagcg ttgctcggac acctatgtta     1380

agttttgaag atggcatatt tattgaggtc accaagaaaa acaagtga                  1428


<210>  3
<211>  478
<212>  PRT
<213>  Bupleurum falcatum

<400>  3

Met Glu Leu Ser Ile Thr Leu Met Leu Ile Phe Ser Thr Thr Ile Phe 
1               5                   10                  15      


Phe Ile Phe Arg Asn Val Tyr Asn His Leu Ile Ser Lys His Lys Asn 
            20                  25                  30          


Tyr Pro Pro Gly Ser Met Gly Leu Pro Tyr Ile Gly Glu Thr Leu Ser 
        35                  40                  45              


Phe Ala Arg Tyr Ile Thr Lys Gly Val Pro Glu Lys Phe Val Ile Glu 
    50                  55                  60                  


Arg Gln Lys Lys Tyr Ser Thr Thr Ile Phe Lys Thr Ser Leu Phe Gly 
65                  70                  75                  80  


Glu Asn Met Val Val Leu Gly Ser Ala Glu Gly Asn Lys Phe Ile Phe 
                85                  90                  95      


Gly Ser Glu Glu Lys Tyr Leu Arg Val Trp Phe Pro Ser Ser Val Asp 
            100                 105                 110         


Lys Val Phe Lys Lys Ser His Lys Arg Thr Ser Gln Glu Glu Ala Ile 
        115                 120                 125             


Arg Leu Arg Lys Asn Met Val Pro Phe Leu Lys Ala Asp Leu Leu Arg 
    130                 135                 140                 


Ser Tyr Val Pro Ile Met Asp Thr Phe Met Lys Gln His Val Asn Ser 
145                 150                 155                 160 


His Trp Asn Cys Glu Thr Leu Lys Ala Cys Pro Val Ile Lys Asp Phe 
                165                 170                 175     


Thr Phe Thr Leu Ala Cys Lys Leu Phe Phe Ser Val Asp Asn Pro Leu 
            180                 185                 190         


Glu Leu Glu Lys Leu Ile Lys Leu Phe Val Asn Ile Val Asn Gly Leu 
        195                 200                 205             


Leu Thr Val Pro Ile Asp Leu Pro Gly Thr Lys Phe Arg Gly Val Ile 
    210                 215                 220                 


Lys Ser Val Lys Thr Ile Arg His Ala Leu Lys Val Leu Ile Arg Gln 
225                 230                 235                 240 


Arg Lys Val Asp Ile Arg Glu Lys Arg Ala Thr Pro Thr Gln Asp Ile 
                245                 250                 255     


Leu Ser Ile Met Leu Ala Gln Ala Glu Asp Glu Asn Tyr Glu Met Asn 
            260                 265                 270         


Asp Glu Asp Val Ala Asn Asp Phe Leu Ala Val Leu Leu Ala Ser Tyr 
        275                 280                 285             


Asp Ser Ala Asn Thr Thr Leu Thr Met Ile Met Lys Tyr Leu Ala Glu 
    290                 295                 300                 


Tyr Pro Glu Met Tyr Asp Arg Val Phe Arg Glu Gln Met Glu Val Ala 
305                 310                 315                 320 


Lys Thr Lys Gly Lys Asp Glu Leu Leu Asn Leu Asp Asp Leu Gln Lys 
                325                 330                 335     


Met Asn Tyr Thr Trp Asn Val Ala Cys Glu Val Leu Arg Ile Ala Thr 
            340                 345                 350         


Pro Thr Phe Gly Ala Phe Arg Glu Val Ile Ala Asp Cys Thr Tyr Glu 
        355                 360                 365             


Gly Tyr Thr Ile Pro Lys Gly Trp Lys Leu Tyr Tyr Ala Pro Arg Phe 
    370                 375                 380                 


Thr His Gly Ser Ala Lys Tyr Phe Gln Asp Pro Glu Lys Phe Asp Pro 
385                 390                 395                 400 


Ser Arg Phe Glu Gly Asp Gly Ala Pro Pro Tyr Thr Phe Val Pro Phe 
                405                 410                 415     


Gly Gly Gly Leu Arg Met Cys Pro Gly Tyr Lys Tyr Ala Lys Ile Ile 
            420                 425                 430         


Val Leu Val Phe Met His Asn Ile Val Thr Lys Phe Lys Trp Glu Lys 
        435                 440                 445             


Val Asn Pro Asn Glu Lys Met Thr Val Gly Ile Val Ser Ala Pro Ser 
    450                 455                 460                 


Gln Gly Leu Pro Leu Arg Leu His Pro His Lys Ser Pro Ser 
465                 470                 475             


<210>  4
<211>  475
<212>  PRT
<213>  Maesa lanceolata

<400>  4

Met Trp Val Val Gly Leu Ile Gly Val Ala Val Val Thr Ile Leu Ile 
1               5                   10                  15      


Thr Gln Tyr Val Tyr Lys Trp Arg Asn Pro Lys Thr Val Gly Val Leu 
            20                  25                  30          


Pro Pro Gly Ser Met Gly Leu Pro Leu Ile Gly Glu Thr Leu Gln Leu 
        35                  40                  45              


Leu Ser Arg Asn Pro Ser Leu Asp Leu His Pro Phe Ile Lys Ser Arg 
    50                  55                  60                  


Ile Gln Arg Tyr Gly Gln Ile Phe Ala Thr Asn Ile Val Gly Arg Pro 
65                  70                  75                  80  


Ile Ile Val Thr Ala Asp Pro Gln Leu Asn Asn Tyr Leu Phe Gln Gln 
                85                  90                  95      


Glu Gly Arg Ala Val Glu Leu Trp Tyr Leu Asp Ser Phe Gln Lys Leu 
            100                 105                 110         


Phe Asn Leu Glu Gly Ala Asn Arg Pro Asn Ala Val Gly His Ile His 
        115                 120                 125             


Lys Tyr Val Arg Ser Val Tyr Leu Ser Leu Phe Gly Val Glu Ser Leu 
    130                 135                 140                 


Lys Thr Lys Leu Leu Ala Asp Ile Glu Lys Thr Val Arg Lys Asn Leu 
145                 150                 155                 160 


Ile Gly Gly Thr Thr Lys Gly Thr Phe Asp Ala Lys His Ala Ser Ala 
                165                 170                 175     


Asn Met Val Ala Val Phe Ala Ala Lys Tyr Leu Phe Gly His Asp Tyr 
            180                 185                 190         


Glu Lys Ser Lys Glu Asp Val Gly Ser Ile Ile Asp Asn Phe Val Gln 
        195                 200                 205             


Gly Leu Leu Ala Phe Pro Leu Asn Val Pro Gly Thr Lys Phe His Lys 
    210                 215                 220                 


Cys Met Lys Asp Lys Lys Arg Leu Glu Ser Met Ile Thr Asn Lys Leu 
225                 230                 235                 240 


Lys Glu Arg Ile Ala Asp Pro Asn Ser Gly Gln Gly Asp Phe Leu Asp 
                245                 250                 255     


Gln Ala Val Lys Asp Leu Asn Ser Glu Phe Phe Ile Thr Glu Thr Phe 
            260                 265                 270         


Ile Val Ser Val Thr Met Gly Ala Leu Phe Ala Thr Val Glu Ser Val 
        275                 280                 285             


Ser Thr Ala Ile Gly Leu Ala Phe Lys Phe Phe Ala Glu His Pro Trp 
    290                 295                 300                 


Val Leu Asp Asp Leu Lys Ala Glu His Glu Ala Val Leu Ser Lys Arg 
305                 310                 315                 320 


Glu Asp Arg Asn Ser Pro Leu Thr Trp Asp Glu Tyr Arg Ser Met Thr 
                325                 330                 335     


His Thr Met His Phe Ile Asn Glu Val Val Arg Leu Gly Asn Val Phe 
            340                 345                 350         


Pro Gly Ile Leu Arg Lys Ala Leu Lys Asp Ile Pro Tyr Asn Gly Tyr 
        355                 360                 365             


Thr Ile Pro Ser Gly Trp Thr Ile Met Ile Val Thr Ser Thr Leu Ala 
    370                 375                 380                 


Met Asn Pro Glu Ile Phe Lys Asp Pro Leu Ala Phe Asn Pro Lys Arg 
385                 390                 395                 400 


Trp Arg Asp Ile Asp Pro Glu Thr Gln Thr Lys Asn Phe Met Pro Phe 
                405                 410                 415     


Gly Gly Gly Thr Arg Gln Cys Ala Gly Ala Glu Leu Ala Lys Ala Phe 
            420                 425                 430         


Phe Ala Thr Phe Leu His Val Leu Ile Ser Glu Tyr Ser Trp Lys Lys 
        435                 440                 445             


Val Lys Gly Gly Ser Val Ala Arg Thr Pro Met Leu Ser Phe Glu Asp 
    450                 455                 460                 


Gly Ile Phe Ile Glu Val Thr Lys Lys Asn Lys 
465                 470                 475 


<210>  5
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  5
ggggacaagt ttgtacaaaa aagcaggctt aatggaactt tctatcact                   49


<210>  6
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  6
ggggaccact ttgtacaaga aagctgggta ttaagatgga gatttgtg                    48


<210>  7
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  7
ggggaccact ttgtacaaga aagctgggta ttaagctttg tgtggataaa ggcg             54


<210>  8
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  8
accgcaugtt agcagacttc ctctgccctc agatggagat ttgtggggat                  50


<210>  9
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  9
atgcggugac gtcgaggaga atcctggccc aatggagcct aatttctatc                  50


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  10
ctcttgcatt caatccgaaa c                                                 21


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  11
agcaaagaat gccttggcta                                                   20


<210>  12
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  12
ggggacaagt ttgtacaaaa aagcaggctt aatgtgggta gtgggatta                   49


<210>  13
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  13
ggggaccact ttgtacaaga aagctgggta tcacttgttt ttcttggt                    48


<210>  14
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  14
ggggacaagt ttgtacaaaa aagcaggctt actcgagatg tggaagctga agatagca         58


<210>  15
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  15
ggggaccact ttgtacaaga aagctgggtt gctagctcaa ttggagagcc acaagcg          57


<210>  16
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  16
ggggacaagt ttgtacaaaa aagcaggctt aatggaagta cattgggttt g                51


<210>  17
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  17
ggggaccact ttgtacaaga aagctgggta ctaagcacat gaaaccttta                  50


<210>  18
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  18
accgcaugtt agcagacttc ctctgccctc cttgtttttc ttggtgacct                  50


<210>  19
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  19
atgcggugac gtcgaggaga atcctggccc aatggaagta cattgggttt                  50


<210>  20
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  20
cctccttata cattcgttcc attc                                              24


<210>  21
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  21
ttagggtcta ctttctccca tttg                                              24


