                         SEQUENCE LISTING

<110>  Aeras Global TB Vaccine Foundation
       Inserm Transfert
 
<120>  RECOMBINANT MYCOBACTERIUM ENCODING A HEPARIN-BINDING 
       HEMAGGGLUTININ (HBHA) FUSION PROTEIN AND USES THEREOF

<130>  08560085ta

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  825
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic recombinant DNA sequence encoding fusion protein

<400>  1
atgagacgac tttgcgcccg aatcgacatt tggcctccac acacggtatg ttctggcccg       60

agcacacgac gacatacagg acaaaggggc acaagtatgg ccacagacgt gagccgaaag      120

attcgagctt ggggacgccg attgatgatc ggcacggcag cggctgtagt ccttccgggc      180

ctggtggggc ttgccggcgg agcggcaacc gcgggcgcgt tctccatggc tgaaaactcg      240

aacattgatg acatcaaggc tccgttgctt gccgcgcttg gagcggccga cctggccttg      300

gccactgtca acgagttgat cacgaacctg cgtgagcgtg cggaggagac tcgtacggac      360

acccgcagcc gggtcgagga gagccgtgct cgcctgacca agctgcagga agatctgccc      420

gagcagctca ccgagctgcg tgagaagttc accgccgagg agctgcgtaa ggccgccgag      480

ggctacctcg aggccgcgac tagccggtac aacgagctgg tcgagcgcgg tgaggccgct      540

ctagagcggc tgcgcagcca gcagagcttc gaggaagtgt cggcgcgcgc cgaaggctac      600

gtggaccagg cggtggagtt gacccaggag gcgttgggta cggtcgcatc gcagacccgc      660

gcggtcggtg agcgtgccgc caagctggtc ggcatcgagc tgcctaagaa ggctgctccg      720

gccaagaagg ccgctccggc caagaaggcc gctccggcca agaaggcggc ggccaagaag      780

gcgcccgcga agaaggcggc ggccaagaag gtcacccaga agtag                      825


<210>  2
<211>  1009
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic recombinant DNA sequence encoding fusion protein

<400>  2
gtggtcttcg tcggcttgct tcgagcgagc ctacgcggtg aacgcaagtt cggcctccct       60

gggggagcac agccggtagc cccgggccgc gattctgaga aatccgcgat agatccatac      120

cgccataccg tttgtgagcc ccctaagcac acttgctctg tccgcggcgg taaccgatac      180

ggaaatgaga cgactttgcg cccgaatcga catttggcct ccacacacgg tatgttctgg      240

cccgagcaca cgacgacata caggacaaag gggcacaagt atggccacag acgtgagccg      300

aaagattcga gcttggggac gccgattgat gatcggcacg gcagcggctg tagtccttcc      360

gggcctggtg gggcttgccg gcggagcggc aaccgcgggc gcgttctcca tggctgaaaa      420

ctcgaacatt gatgacatca aggctccgtt gcttgccgcg cttggagcgg ccgacctggc      480

cttggccact gtcaacgagt tgatcacgaa cctgcgtgag cgtgcggagg agactcgtac      540

ggacacccgc agccgggtcg aggagagccg tgctcgcctg accaagctgc aggaagatct      600

gcccgagcag ctcaccgagc tgcgtgagaa gttcaccgcc gaggagctgc gtaaggccgc      660

cgagggctac ctcgaggccg cgactagccg gtacaacgag ctggtcgagc gcggtgaggc      720

cgctctagag cggctgcgca gccagcagag cttcgaggaa gtgtcggcgc gcgccgaagg      780

ctacgtggac caggcggtgg agttgaccca ggaggcgttg ggtacggtcg catcgcagac      840

ccgcgcggtc ggtgagcgtg ccgccaagct ggtcggcatc gagctgccta agaaggctgc      900

tccggccaag aaggccgctc cggccaagaa ggccgctccg gccaagaagg cggcggccaa      960

gaaggcgccc gcgaagaagg cggcggccaa gaaggtcacc cagaagtag                 1009


<210>  3
<211>  274
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic recombinant fusion protein

<400>  3

Met Arg Arg Leu Cys Ala Arg Ile Asp Ile Trp Pro Pro His Thr Val 
1               5                   10                  15      


Cys Ser Gly Pro Ser Thr Arg Arg His Thr Gly Gln Arg Gly Thr Ser 
            20                  25                  30          


Met Ala Thr Asp Val Ser Arg Lys Ile Arg Ala Trp Gly Arg Arg Leu 
        35                  40                  45              


Met Ile Gly Thr Ala Ala Ala Val Val Leu Pro Gly Leu Val Gly Leu 
    50                  55                  60                  


Ala Gly Gly Ala Ala Thr Ala Gly Ala Phe Ser Met Ala Glu Asn Ser 
65                  70                  75                  80  


Asn Ile Asp Asp Ile Lys Ala Pro Leu Leu Ala Ala Leu Gly Ala Ala 
                85                  90                  95      


Asp Leu Ala Leu Ala Thr Val Asn Glu Leu Ile Thr Asn Leu Arg Glu 
            100                 105                 110         


Arg Ala Glu Glu Thr Arg Thr Asp Thr Arg Ser Arg Val Glu Glu Ser 
        115                 120                 125             


Arg Ala Arg Leu Thr Lys Leu Gln Glu Asp Leu Pro Glu Gln Leu Thr 
    130                 135                 140                 


Glu Leu Arg Glu Lys Phe Thr Ala Glu Glu Leu Arg Lys Ala Ala Glu 
145                 150                 155                 160 


Gly Tyr Leu Glu Ala Ala Thr Ser Arg Tyr Asn Glu Leu Val Glu Arg 
                165                 170                 175     


Gly Glu Ala Ala Leu Glu Arg Leu Arg Ser Gln Gln Ser Phe Glu Glu 
            180                 185                 190         


Val Ser Ala Arg Ala Glu Gly Tyr Val Asp Gln Ala Val Glu Leu Thr 
        195                 200                 205             


Gln Glu Ala Leu Gly Thr Val Ala Ser Gln Thr Arg Ala Val Gly Glu 
    210                 215                 220                 


Arg Ala Ala Lys Leu Val Gly Ile Glu Leu Pro Lys Lys Ala Ala Pro 
225                 230                 235                 240 


Ala Lys Lys Ala Ala Pro Ala Lys Lys Ala Ala Pro Ala Lys Lys Ala 
                245                 250                 255     


Ala Ala Lys Lys Ala Pro Ala Lys Lys Ala Ala Ala Lys Lys Val Thr 
            260                 265                 270         


Gln Lys 
        


<210>  4
<211>  198
<212>  PRT
<213>  Mycobacterium tuberculosis

<400>  4

Ala Glu Asn Ser Asn Ile Asp Asp Ile Lys Ala Pro Leu Leu Ala Ala 
1               5                   10                  15      


Leu Gly Ala Ala Asp Leu Ala Leu Ala Thr Val Asn Glu Leu Ile Thr 
            20                  25                  30          


Asn Leu Arg Glu Arg Ala Glu Glu Thr Arg Thr Asp Thr Arg Ser Arg 
        35                  40                  45              


Val Glu Glu Ser Arg Ala Arg Leu Thr Lys Leu Gln Glu Asp Leu Pro 
    50                  55                  60                  


Glu Gln Leu Thr Glu Leu Arg Glu Lys Phe Thr Ala Glu Glu Leu Arg 
65                  70                  75                  80  


Lys Ala Ala Glu Gly Tyr Leu Glu Ala Ala Thr Ser Arg Tyr Asn Glu 
                85                  90                  95      


Leu Val Glu Arg Gly Glu Ala Ala Leu Glu Arg Leu Arg Ser Gln Gln 
            100                 105                 110         


Ser Phe Glu Glu Val Ser Ala Arg Ala Glu Gly Tyr Val Asp Gln Ala 
        115                 120                 125             


Val Glu Leu Thr Gln Glu Ala Leu Gly Thr Val Ala Ser Gln Thr Arg 
    130                 135                 140                 


Ala Val Gly Glu Arg Ala Ala Lys Leu Val Gly Ile Glu Leu Pro Lys 
145                 150                 155                 160 


Lys Ala Ala Pro Ala Lys Lys Ala Ala Pro Ala Lys Lys Ala Ala Pro 
                165                 170                 175     


Ala Lys Lys Ala Ala Ala Lys Lys Ala Pro Ala Lys Lys Ala Ala Ala 
            180                 185                 190         


Lys Lys Val Thr Gln Lys 
        195             


<210>  5
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide of carboxy terminal heparin binding region of 
       HBHA

<400>  5

Lys Lys Ala Ala Pro Ala Lys Lys Ala Ala Pro Ala Lys Lys Ala Ala 
1               5                   10                  15      


Pro Ala Lys Lys Ala Ala Ala Lys Lys Ala Pro Ala Lys Lys Ala Ala 
            20                  25                  30          


Ala Lys Lys Val Thr Gln Lys 
        35                  


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  6
ggtcttcgtc ggcttgcttc                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  7
gctctgccag tgttacaacc                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  8
gtctacgagg ccacactcag                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide sequence

<400>  9
tatcgcgcag ctccaggtag                                                   20


<210>  10
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  10
gctcgaggcc gcgattaaat tc                                                22


<210>  11
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  11
ggatggcaag atcctggtat cg                                                22


<210>  12
<211>  1031
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic recombinant DNA sequence encoding fusion protein

<400>  12
actgttaatt aagtggtctt cgtcggcttg cttcgagcga gcctacgcgg tgaacgcaag       60

ttcggcctcc ctgggggagc acagccggta gccccgggcc gcgattctga gaaatccgcg      120

atagatccat accgccatac cgtttgtgag ccccctaagc acacttgctc tgtccgcggc      180

ggtaaccgat acggaaatga gacgactttg cgcccgaatc gacatttggc ctccacacac      240

ggtatgttct ggcccgagca cacgacgaca tacaggacaa aggggcacaa gtatggccac      300

agacgtgagc cgaaagattc gagcttgggg acgccgattg atgatcggca cggcagcggc      360

tgtagtcctt ccgggcctgg tggggcttgc cggcggagcg gcaaccgcgg gcgcgttctc      420

catggctgaa aactcgaaca ttgatgacat caaggctccg ttgcttgccg cgcttggagc      480

ggccgacctg gccttggcca ctgtcaacga gttgatcacg aacctgcgtg agcgtgcgga      540

ggagactcgt acggacaccc gcagccgggt cgaggagagc cgtgctcgcc tgaccaagct      600

gcaggaagat ctgcccgagc agctcaccga gctgcgtgag aagttcaccg ccgaggagct      660

gcgtaaggcc gccgagggct acctcgaggc cgcgactagc cggtacaacg agctggtcga      720

gcgcggtgag gccgctctag agcggctgcg cagccagcag agcttcgagg aagtgtcggc      780

gcgcgccgaa ggctacgtgg accaggcggt ggagttgacc caggaggcgt tgggtacggt      840

cgcatcgcag acccgcgcgg tcggtgagcg tgccgccaag ctggtcggca tcgagctgcc      900

taagaaggct gctccggcca agaaggccgc tccggccaag aaggccgctc cggccaagaa      960

ggcggcggcc aagaaggcgc ccgcgaagaa ggcggcggcc aagaaggtca cccagaagta     1020

gactagttca t                                                          1031


