                         SEQUENCE LISTING

<110>  Evonik Industries AG
 
<120>  NADP-abhngige Alanindehydrogenase

<130>  201200079

<160>  23    

<170>  PatentIn version 3.5

<210>  1
<211>  378
<212>  PRT
<213>  Bacillus subtilis

<400>  1

Met Ile Ile Gly Val Pro Lys Glu Ile Lys Asn Asn Glu Asn Arg Val 
1               5                   10                  15      


Ala Leu Thr Pro Gly Gly Val Ser Gln Leu Ile Ser Asn Gly His Arg 
            20                  25                  30          


Val Leu Val Glu Thr Gly Ala Gly Leu Gly Ser Gly Phe Glu Asn Glu 
        35                  40                  45              


Ala Tyr Glu Ser Ala Gly Ala Glu Ile Ile Ala Asp Pro Lys Gln Val 
    50                  55                  60                  


Trp Asp Ala Glu Met Val Met Lys Val Lys Glu Pro Leu Pro Glu Glu 
65                  70                  75                  80  


Tyr Val Tyr Phe Arg Lys Gly Leu Val Leu Phe Thr Tyr Leu His Leu 
                85                  90                  95      


Ala Ala Glu Pro Glu Leu Ala Gln Ala Leu Lys Asp Lys Gly Val Thr 
            100                 105                 110         


Ala Ile Ala Tyr Glu Thr Val Ser Glu Gly Arg Thr Leu Pro Leu Leu 
        115                 120                 125             


Thr Pro Met Ser Glu Val Ala Gly Arg Met Ala Ala Gln Ile Gly Ala 
    130                 135                 140                 


Gln Phe Leu Glu Lys Pro Lys Gly Gly Lys Gly Ile Leu Leu Ala Gly 
145                 150                 155                 160 


Val Pro Gly Val Ser Arg Gly Lys Val Thr Ile Ile Gly Gly Gly Val 
                165                 170                 175     


Val Gly Thr Asn Ala Ala Lys Met Ala Val Gly Leu Gly Ala Asp Val 
            180                 185                 190         


Thr Ile Ile Ala Arg Asn Ala Asp Arg Leu Arg Gln Leu Asp Asp Ile 
        195                 200                 205             


Phe Gly His Gln Ile Lys Thr Leu Ile Ser Asn Pro Val Asn Ile Ala 
    210                 215                 220                 


Asp Ala Val Ala Glu Ala Asp Leu Leu Ile Cys Ala Val Leu Ile Pro 
225                 230                 235                 240 


Gly Ala Lys Ala Pro Thr Leu Val Thr Glu Glu Met Val Lys Gln Met 
                245                 250                 255     


Lys Pro Gly Ser Val Ile Val Asp Val Ala Ile Asp Gln Gly Gly Ile 
            260                 265                 270         


Val Glu Thr Val Asp His Ile Thr Thr His Asp Gln Pro Thr Tyr Glu 
        275                 280                 285             


Lys His Gly Val Val His Tyr Ala Val Ala Asn Met Pro Gly Ala Val 
    290                 295                 300                 


Pro Arg Thr Ser Thr Ile Ala Leu Thr Asn Val Thr Val Pro Tyr Ala 
305                 310                 315                 320 


Leu Gln Ile Ala Asn Lys Gly Ala Val Lys Ala Leu Ala Asp Asn Thr 
                325                 330                 335     


Ala Leu Arg Ala Gly Leu Asn Thr Ala Asn Gly His Val Thr Tyr Glu 
            340                 345                 350         


Ala Val Ala Arg Asp Leu Gly Tyr Glu Tyr Val Pro Ala Glu Lys Ala 
        355                 360                 365             


Leu Gln Asp Glu Ser Ser Val Ala Gly Ala 
    370                 375             


<210>  2
<211>  1137
<212>  DNA
<213>  Bacillus subtilis

<400>  2
atgatcatag gggttcctaa agagataaaa aacaatgaaa accgtgtcgc attaacaccc       60

gggggcgttt ctcagctcat ttcaaacggc caccgggtgc tggttgaaac aggcgcgggc      120

cttggaagcg gatttgaaaa tgaagcctat gagtcagcag gagcggaaat cattgctgat      180

ccgaagcagg tctgggacgc cgaaatggtc atgaaagtaa aagaaccgct gccggaagaa      240

tatgtttatt ttcgcaaagg acttgtgctg tttacgtacc ttcatttagc agctgagcct      300

gagcttgcac aggccttgaa ggataaagga gtaactgcca tcgcatatga aacggtcagt      360

gaaggccgga cattgcctct tctgacgcca atgtcagagg ttgcgggcag aatggcagcg      420

caaatcggcg ctcaattctt agaaaagcct aaaggcggaa aaggcattct gcttgccggg      480

gtgcctggcg tttcccgcgg aaaagtaaca attatcggag gaggcgttgt cgggacaaac      540

gcggcgaaaa tggctgtcgg cctcggtgca gatgtgacga tcattgcccg taacgcagac      600

cgcttgcgcc agcttgatga catcttcggc catcagatta aaacgttaat ttctaatccg      660

gtcaatattg ctgatgctgt ggcggaagcg gatctcctca tttgcgcggt attaattccg      720

ggtgctaaag ctccgactct tgtcactgag gaaatggtaa aacaaatgaa acccggttca      780

gttattgttg atgtagcgat cgaccaaggc ggcatcgtcg aaactgtcga ccatatcaca      840

acacatgatc agccaacata tgaaaaacac ggggttgtgc attatgctgt agcgaacatg      900

ccaggcgcag tccctcgtac atcaacaatc gccctgacta acgttactgt tccatacgcg      960

ctgcaaatcg cgaacaaagg ggcagtaaaa gcgctcgcag acaatacggc actgagagcg     1020

ggtttaaaca ccgcaaacgg acacgtgacc tatgaagctg tagcaagaga tctaggctat     1080

gagtatgttc ctgccgagaa agctttacag gatgaatcat ctgtggcggg tgcttaa        1137


<210>  3
<211>  353
<212>  PRT
<213>  E. coli

<400>  3

Met Leu Tyr Thr Ser Gln Thr Thr Pro Glu Lys Asp Gln Lys Met Ser 
1               5                   10                  15      


Met Ile Lys Ser Tyr Ala Ala Lys Glu Ala Gly Gly Glu Leu Glu Val 
            20                  25                  30          


Tyr Glu Tyr Asp Pro Gly Glu Leu Arg Pro Gln Asp Val Glu Val Gln 
        35                  40                  45              


Val Asp Tyr Cys Gly Ile Cys His Ser Asp Leu Ser Met Ile Asp Asn 
    50                  55                  60                  


Glu Trp Gly Phe Ser Gln Tyr Pro Leu Val Ala Gly His Glu Val Ile 
65                  70                  75                  80  


Gly Arg Val Val Ala Leu Gly Ser Ala Ala Gln Asp Lys Gly Leu Gln 
                85                  90                  95      


Val Gly Gln Arg Val Gly Ile Gly Trp Thr Ala Arg Ser Cys Gly His 
            100                 105                 110         


Cys Asp Ala Cys Ile Ser Gly Asn Gln Ile Asn Cys Glu Gln Gly Ala 
        115                 120                 125             


Val Pro Thr Ile Met Asn Arg Gly Gly Phe Ala Glu Lys Leu Arg Ala 
    130                 135                 140                 


Asp Trp Gln Trp Val Ile Pro Leu Pro Glu Asn Ile Asp Ile Glu Ser 
145                 150                 155                 160 


Ala Gly Pro Leu Leu Cys Gly Gly Ile Thr Val Phe Lys Pro Leu Leu 
                165                 170                 175     


Met His His Ile Thr Ala Thr Ser Arg Val Gly Val Ile Gly Ile Gly 
            180                 185                 190         


Gly Leu Gly His Ile Ala Ile Lys Leu Leu His Ala Met Gly Cys Glu 
        195                 200                 205             


Val Thr Ala Phe Ser Ser Asn Pro Ala Lys Glu Gln Glu Val Leu Ala 
    210                 215                 220                 


Met Gly Ala Asp Lys Val Val Asn Ser Arg Asp Pro Gln Ala Leu Lys 
225                 230                 235                 240 


Ala Leu Ala Gly Gln Phe Asp Leu Ile Ile Asn Thr Val Asn Val Ser 
                245                 250                 255     


Leu Asp Trp Gln Pro Tyr Phe Glu Ala Leu Thr Tyr Gly Gly Asn Phe 
            260                 265                 270         


His Thr Val Gly Ala Val Leu Thr Pro Leu Ser Val Pro Ala Phe Thr 
        275                 280                 285             


Leu Ile Ala Gly Asp Arg Ser Val Ser Gly Ser Ala Thr Gly Thr Pro 
    290                 295                 300                 


Tyr Glu Leu Arg Lys Leu Met Arg Phe Ala Ala Arg Ser Lys Val Ala 
305                 310                 315                 320 


Pro Thr Thr Glu Leu Phe Pro Met Ser Lys Ile Asn Asp Ala Ile Gln 
                325                 330                 335     


His Val Arg Asp Gly Lys Ala Arg Tyr Arg Val Val Leu Lys Ala Asp 
            340                 345                 350         


Phe 
    


<210>  4
<211>  349
<212>  PRT
<213>  E. coli

<400>  4

Met Lys Ile Lys Ala Val Gly Ala Tyr Ser Ala Lys Gln Pro Leu Glu 
1               5                   10                  15      


Pro Met Asp Ile Thr Arg Arg Glu Pro Gly Pro Asn Asp Val Lys Ile 
            20                  25                  30          


Glu Ile Ala Tyr Cys Gly Val Cys His Ser Asp Leu His Gln Val Arg 
        35                  40                  45              


Ser Glu Trp Ala Gly Thr Val Tyr Pro Cys Val Pro Gly His Glu Ile 
    50                  55                  60                  


Val Gly Arg Val Val Ala Val Gly Asp Gln Val Glu Lys Tyr Ala Pro 
65                  70                  75                  80  


Gly Asp Leu Val Gly Val Gly Cys Ile Val Asp Ser Cys Lys His Cys 
                85                  90                  95      


Glu Glu Cys Glu Asp Gly Leu Glu Asn Tyr Cys Asp His Met Thr Gly 
            100                 105                 110         


Thr Tyr Asn Ser Pro Thr Pro Asp Glu Pro Gly His Thr Leu Gly Gly 
        115                 120                 125             


Tyr Ser Gln Gln Ile Val Val His Glu Arg Tyr Val Leu Arg Ile Arg 
    130                 135                 140                 


His Pro Gln Glu Gln Leu Ala Ala Val Ala Pro Leu Leu Cys Ala Gly 
145                 150                 155                 160 


Ile Thr Thr Tyr Ser Pro Leu Arg His Trp Gln Ala Gly Pro Gly Lys 
                165                 170                 175     


Lys Val Gly Val Val Gly Ile Gly Gly Leu Gly His Met Gly Ile Lys 
            180                 185                 190         


Leu Ala His Ala Met Gly Ala His Val Val Ala Phe Thr Thr Ser Glu 
        195                 200                 205             


Ala Lys Arg Glu Ala Ala Lys Ala Leu Gly Ala Asp Glu Val Val Asn 
    210                 215                 220                 


Ser Arg Asn Ala Asp Glu Met Ala Ala His Leu Lys Ser Phe Asp Phe 
225                 230                 235                 240 


Ile Leu Asn Thr Val Ala Ala Pro His Asn Leu Asp Asp Phe Thr Thr 
                245                 250                 255     


Leu Leu Lys Arg Asp Gly Thr Met Thr Leu Val Gly Ala Pro Ala Thr 
            260                 265                 270         


Pro His Lys Ser Pro Glu Val Phe Asn Leu Ile Met Lys Arg Arg Ala 
        275                 280                 285             


Ile Ala Gly Ser Met Ile Gly Gly Ile Pro Glu Thr Gln Glu Met Leu 
    290                 295                 300                 


Asp Phe Cys Ala Glu His Gly Ile Val Ala Asp Ile Glu Met Ile Arg 
305                 310                 315                 320 


Ala Asp Gln Ile Asn Glu Ala Tyr Glu Arg Met Leu Arg Gly Asp Val 
                325                 330                 335     


Lys Tyr Arg Phe Val Ile Asp Asn Arg Thr Leu Thr Asp 
            340                 345                 


<210>  5
<211>  789
<212>  DNA
<213>  Ralstonia sp.

<400>  5
atggctagca gaggatcgca tcaccatcac catcacggcg cctatcgact attaaacaaa       60

acagccgtca taaccggtgg aaacagcggc attggcctcg ccacagcgaa gcgcttcgtt      120

gccgagggtg cctatgtatt cattgtcggt cgccggcgga aggaactcga gcaggcggcc      180

gcagaaatcg gtcggaatgt cacggcggtc aaagccgatg tgacaaagct tgaagacctg      240

gaccgacttt acgcgattgt gcgtgagcaa cggggtagca tcgacgtact atttgcgaat      300

tccggcgcaa tcgagcaaaa gacgcttgag gagattactc cggaacacta tgacaggact      360

ttcgatgtca acgttcgggg attgatcttc accgtgcaga aggcacttcc tctgctgcga      420

gacggcggca gcgtgatcct gacaagctcg gtagccggcg tcctaggatt acaggcgcac      480

gacacgtata gtgccgccaa ggcagcggta aggtcgctcg cgaggacatg gaccactgag      540

ttgaaaggtc gcagcattcg tgtcaacgcg gtaagcccag gggcgatcga cacgcctatc      600

atagaaaacc aggtctctac acaggaagaa gctgacgagc tgcgtgcgaa atttgcagct      660

gcgacgcccc tgggtcgcgt cggacgacct gaagagctgg cagcggccgt gttatttctt      720

gcatcggacg acagtagcta cgtagccggc attgagctgt ttgtggacgg tggattgacc      780

caggtctaa                                                              789


<210>  6
<211>  459
<212>  PRT
<213>  Chromobacterium violaceum ATCC 12472

<400>  6

Met Gln Lys Gln Arg Thr Thr Ser Gln Trp Arg Glu Leu Asp Ala Ala 
1               5                   10                  15      


His His Leu His Pro Phe Thr Asp Thr Ala Ser Leu Asn Gln Ala Gly 
            20                  25                  30          


Ala Arg Val Met Thr Arg Gly Glu Gly Val Tyr Leu Trp Asp Ser Glu 
        35                  40                  45              


Gly Asn Lys Ile Ile Asp Gly Met Ala Gly Leu Trp Cys Val Asn Val 
    50                  55                  60                  


Gly Tyr Gly Arg Lys Asp Phe Ala Glu Ala Ala Arg Arg Gln Met Glu 
65                  70                  75                  80  


Glu Leu Pro Phe Tyr Asn Thr Phe Phe Lys Thr Thr His Pro Ala Val 
                85                  90                  95      


Val Glu Leu Ser Ser Leu Leu Ala Glu Val Thr Pro Ala Gly Phe Asp 
            100                 105                 110         


Arg Val Phe Tyr Thr Asn Ser Gly Ser Glu Ser Val Asp Thr Met Ile 
        115                 120                 125             


Arg Met Val Arg Arg Tyr Trp Asp Val Gln Gly Lys Pro Glu Lys Lys 
    130                 135                 140                 


Thr Leu Ile Gly Arg Trp Asn Gly Tyr His Gly Ser Thr Ile Gly Gly 
145                 150                 155                 160 


Ala Ser Leu Gly Gly Met Lys Tyr Met His Glu Gln Gly Asp Leu Pro 
                165                 170                 175     


Ile Pro Gly Met Ala His Ile Glu Gln Pro Trp Trp Tyr Lys His Gly 
            180                 185                 190         


Lys Asp Met Thr Pro Asp Glu Phe Gly Val Val Ala Ala Arg Trp Leu 
        195                 200                 205             


Glu Glu Lys Ile Leu Glu Ile Gly Ala Asp Lys Val Ala Ala Phe Val 
    210                 215                 220                 


Gly Glu Pro Ile Gln Gly Ala Gly Gly Val Ile Val Pro Pro Ala Thr 
225                 230                 235                 240 


Tyr Trp Pro Glu Ile Glu Arg Ile Cys Arg Lys Tyr Asp Val Leu Leu 
                245                 250                 255     


Val Ala Asp Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Glu Trp Phe 
            260                 265                 270         


Gly His Gln His Phe Gly Phe Gln Pro Asp Leu Phe Thr Ala Ala Lys 
        275                 280                 285             


Gly Leu Ser Ser Gly Tyr Leu Pro Ile Gly Ala Val Phe Val Gly Lys 
    290                 295                 300                 


Arg Val Ala Glu Gly Leu Ile Ala Gly Gly Asp Phe Asn His Gly Phe 
305                 310                 315                 320 


Thr Tyr Ser Gly His Pro Val Cys Ala Ala Val Ala His Ala Asn Val 
                325                 330                 335     


Ala Ala Leu Arg Asp Glu Gly Ile Val Gln Arg Val Lys Asp Asp Ile 
            340                 345                 350         


Gly Pro Tyr Met Gln Lys Arg Trp Arg Glu Thr Phe Ser Arg Phe Glu 
        355                 360                 365             


His Val Asp Asp Val Arg Gly Val Gly Met Val Gln Ala Phe Thr Leu 
    370                 375                 380                 


Val Lys Asn Lys Ala Lys Arg Glu Leu Phe Pro Asp Phe Gly Glu Ile 
385                 390                 395                 400 


Gly Thr Leu Cys Arg Asp Ile Phe Phe Arg Asn Asn Leu Ile Met Arg 
                405                 410                 415     


Ala Cys Gly Asp His Ile Val Ser Ala Pro Pro Leu Val Met Thr Arg 
            420                 425                 430         


Ala Glu Val Asp Glu Met Leu Ala Val Ala Glu Arg Cys Leu Glu Glu 
        435                 440                 445             


Phe Glu Gln Thr Leu Lys Ala Arg Gly Leu Ala 
    450                 455                 


<210>  7
<211>  452
<212>  PRT
<213>  Pseudomonas putida

<400>  7

Met Ser Glu Gln Asn Ser Gln Thr Gln Ala Trp Gln Ala Leu Ser Arg 
1               5                   10                  15      


Asp His His Leu Ala Pro Phe Ser Asp Val Lys Gln Leu Ala Glu Lys 
            20                  25                  30          


Gly Pro Arg Ile Ile Thr Ser Ala Lys Gly Val Tyr Leu Trp Asp Ser 
        35                  40                  45              


Glu Gly Asn Lys Ile Leu Asp Gly Met Ala Gly Leu Trp Cys Val Ala 
    50                  55                  60                  


Val Gly Tyr Gly Arg Asp Glu Leu Ala Glu Val Ala Ser Gln Gln Met 
65                  70                  75                  80  


Lys Gln Leu Pro Tyr Tyr Asn Leu Phe Phe Gln Thr Ala His Pro Pro 
                85                  90                  95      


Ala Leu Glu Leu Ala Lys Ala Ile Ala Asp Val Ala Pro Gln Gly Met 
            100                 105                 110         


Asn His Val Phe Phe Thr Gly Ser Gly Ser Glu Gly Asn Asp Thr Val 
        115                 120                 125             


Leu Arg Met Val Arg His Tyr Trp Ala Leu Lys Gly Lys Lys Asn Lys 
    130                 135                 140                 


Asn Val Ile Ile Gly Arg Ile Asn Gly Tyr His Gly Ser Thr Val Ala 
145                 150                 155                 160 


Gly Ala Ala Leu Gly Gly Met Ser Gly Met His Gln Gln Gly Gly Val 
                165                 170                 175     


Ile Pro Asp Ile Val His Ile Pro Gln Pro Tyr Trp Phe Gly Glu Gly 
            180                 185                 190         


Gly Asp Met Thr Glu Ala Asp Phe Gly Val Trp Ala Ala Glu Gln Leu 
        195                 200                 205             


Glu Lys Lys Ile Leu Glu Val Gly Val Asp Asn Val Ala Ala Phe Ile 
    210                 215                 220                 


Ala Glu Pro Ile Gln Gly Ala Gly Gly Val Ile Ile Pro Pro Gln Thr 
225                 230                 235                 240 


Tyr Trp Pro Lys Val Lys Glu Ile Leu Ala Arg Tyr Asp Ile Leu Phe 
                245                 250                 255     


Val Ala Asp Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Glu Trp Phe 
            260                 265                 270         


Gly Thr Asp Tyr Tyr Asp Leu Lys Pro Asp Leu Met Thr Ile Ala Lys 
        275                 280                 285             


Gly Leu Thr Ser Gly Tyr Ile Pro Met Gly Gly Val Ile Val Arg Asp 
    290                 295                 300                 


Glu Val Ala Lys Val Ile Ser Glu Gly Gly Asp Phe Asn His Gly Phe 
305                 310                 315                 320 


Thr Tyr Ser Gly His Pro Val Ala Ala Ala Val Gly Leu Glu Asn Leu 
                325                 330                 335     


Arg Ile Leu Arg Asp Glu Gln Ile Ile Gln Gln Val His Asp Lys Thr 
            340                 345                 350         


Ala Pro Tyr Leu Gln Gln Arg Leu Arg Glu Leu Ala Asp His Pro Leu 
        355                 360                 365             


Val Gly Glu Val Arg Gly Leu Gly Met Leu Gly Ala Ile Glu Leu Val 
    370                 375                 380                 


Lys Asp Lys Ala Thr Arg Ala Arg Tyr Glu Gly Lys Gly Val Gly Met 
385                 390                 395                 400 


Ile Cys Arg Gln His Cys Phe Asp Asn Gly Leu Ile Met Arg Ala Val 
                405                 410                 415     


Gly Asp Thr Met Ile Ile Ala Pro Pro Leu Val Ile Ser Val Glu Glu 
            420                 425                 430         


Ile Asp Glu Leu Val Gln Lys Ala Arg Lys Cys Leu Asp Leu Thr Tyr 
        435                 440                 445             


Glu Ala Val Arg 
    450         


<210>  8
<211>  452
<212>  PRT
<213>  Pseudomonas putida

<400>  8

Met Ser Glu Gln Asn Ser Gln Thr Gln Ala Trp Gln Ala Leu Ser Arg 
1               5                   10                  15      


Asp His His Leu Ala Pro Phe Ser Asp Val Lys Gln Leu Ala Glu Lys 
            20                  25                  30          


Gly Pro Arg Ile Ile Thr Ser Ala Lys Gly Val Tyr Leu Trp Asp Ser 
        35                  40                  45              


Glu Gly Asn Lys Ile Leu Asp Gly Met Ala Gly Leu Trp Cys Val Ala 
    50                  55                  60                  


Val Gly Tyr Gly Arg Asp Glu Leu Ala Glu Val Ala Ser Gln Gln Met 
65                  70                  75                  80  


Lys Gln Leu Pro Tyr Tyr Asn Leu Phe Phe Gln Thr Ala His Pro Pro 
                85                  90                  95      


Ala Leu Glu Leu Ala Lys Ala Ile Ala Asp Val Ala Pro Gln Gly Met 
            100                 105                 110         


Asn His Val Phe Phe Thr Gly Ser Gly Ser Glu Gly Asn Asp Thr Val 
        115                 120                 125             


Leu Arg Met Val Arg His Tyr Trp Ala Leu Lys Gly Lys Lys Asn Lys 
    130                 135                 140                 


Asn Val Ile Ile Gly Arg Ile Asn Gly Tyr His Gly Ser Thr Val Ala 
145                 150                 155                 160 


Gly Ala Ala Leu Gly Gly Met Ser Gly Met His Gln Gln Gly Gly Val 
                165                 170                 175     


Ile Pro Asp Ile Val His Ile Pro Gln Pro Tyr Trp Phe Gly Glu Gly 
            180                 185                 190         


Gly Asp Met Thr Glu Ala Asp Phe Gly Val Trp Ala Ala Glu Gln Leu 
        195                 200                 205             


Glu Lys Lys Ile Leu Glu Val Gly Val Asp Asn Val Ala Ala Phe Ile 
    210                 215                 220                 


Ala Glu Pro Ile Gln Gly Ala Gly Gly Val Ile Ile Pro Pro Gln Thr 
225                 230                 235                 240 


Tyr Trp Pro Lys Val Lys Glu Ile Leu Ala Arg Tyr Asp Ile Leu Phe 
                245                 250                 255     


Val Ala Asp Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Glu Trp Phe 
            260                 265                 270         


Gly Thr Asp Tyr Tyr Asp Leu Lys Pro Asp Leu Met Thr Ile Ala Lys 
        275                 280                 285             


Gly Leu Thr Ser Gly Tyr Ile Pro Met Gly Gly Val Ile Val Arg Asp 
    290                 295                 300                 


Glu Val Ala Lys Val Ile Ser Glu Gly Gly Asp Phe Asn His Gly Phe 
305                 310                 315                 320 


Thr Tyr Ser Gly His Pro Val Ala Ala Ala Val Gly Leu Glu Asn Leu 
                325                 330                 335     


Arg Ile Leu Arg Asp Glu Gln Ile Ile Gln Gln Val His Asp Lys Thr 
            340                 345                 350         


Ala Pro Tyr Leu Gln Gln Arg Leu Arg Glu Leu Ala Asp His Pro Leu 
        355                 360                 365             


Val Gly Glu Val Arg Gly Leu Gly Met Leu Gly Ala Ile Glu Leu Val 
    370                 375                 380                 


Lys Asp Lys Ala Thr Arg Ala Arg Tyr Glu Gly Lys Gly Val Gly Met 
385                 390                 395                 400 


Ile Cys Arg Gln His Cys Phe Asp Asn Gly Leu Ile Met Arg Ala Val 
                405                 410                 415     


Gly Asp Thr Met Ile Ile Ala Pro Pro Leu Val Ile Ser Val Glu Glu 
            420                 425                 430         


Ile Asp Glu Leu Val Gln Lys Ala Arg Lys Cys Leu Asp Leu Thr Tyr 
        435                 440                 445             


Glu Ala Val Arg 
    450         


<210>  9
<211>  453
<212>  PRT
<213>  Pseudomonas putida

<400>  9

Met Ser Val Asn Asn Pro Gln Thr Arg Glu Trp Gln Thr Leu Ser Gly 
1               5                   10                  15      


Glu His His Leu Ala Pro Phe Ser Asp Tyr Lys Gln Leu Lys Glu Lys 
            20                  25                  30          


Gly Pro Arg Ile Ile Thr Lys Ala Gln Gly Val His Leu Trp Asp Ser 
        35                  40                  45              


Glu Gly His Lys Ile Leu Asp Gly Met Ala Gly Leu Trp Cys Val Ala 
    50                  55                  60                  


Val Gly Tyr Gly Arg Glu Glu Leu Val Gln Ala Ala Glu Lys Gln Met 
65                  70                  75                  80  


Arg Glu Leu Pro Tyr Tyr Asn Leu Phe Phe Gln Thr Ala His Pro Pro 
                85                  90                  95      


Ala Leu Glu Leu Ala Lys Ala Ile Thr Asp Val Ala Pro Gln Gly Met 
            100                 105                 110         


Thr His Val Phe Phe Thr Gly Ser Gly Ser Glu Gly Asn Asp Thr Val 
        115                 120                 125             


Leu Arg Met Val Arg His Tyr Trp Ala Leu Lys Gly Lys Pro His Lys 
    130                 135                 140                 


Gln Thr Ile Ile Gly Arg Ile Asn Gly Tyr His Gly Ser Thr Phe Ala 
145                 150                 155                 160 


Gly Ala Cys Leu Gly Gly Met Ser Gly Met His Glu Gln Gly Gly Leu 
                165                 170                 175     


Pro Ile Pro Gly Ile Val His Ile Pro Gln Pro Tyr Trp Phe Gly Glu 
            180                 185                 190         


Gly Gly Asp Met Thr Pro Asp Ala Phe Gly Val Trp Ala Ala Glu Gln 
        195                 200                 205             


Leu Glu Lys Lys Ile Leu Glu Val Gly Glu Asp Asn Val Ala Ala Phe 
    210                 215                 220                 


Ile Ala Glu Pro Ile Gln Gly Ala Gly Gly Val Ile Ile Pro Pro Glu 
225                 230                 235                 240 


Thr Tyr Trp Pro Lys Val Lys Glu Ile Leu Ala Lys Tyr Asp Ile Leu 
                245                 250                 255     


Phe Val Ala Asp Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Glu Trp 
            260                 265                 270         


Phe Gly Ser Asp Tyr Tyr Asp Leu Lys Pro Asp Leu Met Thr Ile Ala 
        275                 280                 285             


Lys Gly Leu Thr Ser Gly Tyr Ile Pro Met Gly Gly Val Ile Val Arg 
    290                 295                 300                 


Asp Thr Val Ala Lys Val Ile Ser Glu Gly Gly Asp Phe Asn His Gly 
305                 310                 315                 320 


Phe Thr Tyr Ser Gly His Pro Val Ala Ala Ala Val Gly Leu Glu Asn 
                325                 330                 335     


Leu Arg Ile Leu Arg Asp Glu Lys Ile Val Glu Lys Ala Arg Thr Glu 
            340                 345                 350         


Ala Ala Pro Tyr Leu Gln Lys Arg Leu Arg Glu Leu Gln Asp His Pro 
        355                 360                 365             


Leu Val Gly Glu Val Arg Gly Leu Gly Met Leu Gly Ala Ile Glu Leu 
    370                 375                 380                 


Val Lys Asp Lys Ala Thr Arg Ser Arg Tyr Glu Gly Lys Gly Val Gly 
385                 390                 395                 400 


Met Ile Cys Arg Thr Phe Cys Phe Asp Asn Gly Leu Ile Met Arg Ala 
                405                 410                 415     


Val Gly Asp Thr Met Ile Ile Ala Pro Pro Leu Val Ile Ser His Ala 
            420                 425                 430         


Glu Ile Asp Glu Leu Val Glu Lys Ala Arg Lys Cys Leu Asp Leu Thr 
        435                 440                 445             


Leu Glu Ala Ile Asn 
    450             


<210>  10
<211>  455
<212>  PRT
<213>  Rhodobacter spaeroides

<400>  10

Met Arg Asp Asp Ala Pro Asn Ser Trp Glu Ser Arg Ala Asp Ala Ser 
1               5                   10                  15      


Ser Phe Tyr Gly Phe Thr Asp Leu Pro Ser Val His Gln Arg Gly Thr 
            20                  25                  30          


Val Val Leu Thr His Gly Lys Gly Pro Tyr Ile Tyr Asp Val His Gly 
        35                  40                  45              


Arg Ala Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly 
    50                  55                  60                  


Phe Asp His Pro Gly Leu Ile Glu Ala Ala Lys Ala Gln Tyr Glu Arg 
65                  70                  75                  80  


Phe Pro Gly Tyr His Ala Phe Phe Gly Arg Met Ser Asp Gln Thr Val 
                85                  90                  95      


Met Leu Ser Glu Lys Leu Val Glu Val Ser Pro Phe Ala Arg Gly Arg 
            100                 105                 110         


Val Phe Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys 
        115                 120                 125             


Met Leu Trp Phe Leu Gly Ala Ala Glu Gly His Pro Glu Arg Arg Lys 
    130                 135                 140                 


Ile Ile Thr Arg Val Asn Ser Tyr His Gly Val Thr Ala Val Ser Ala 
145                 150                 155                 160 


Ser Met Thr Gly Lys Pro Tyr Asn Ser Leu Phe Gly Leu Pro Leu Pro 
                165                 170                 175     


Gly Phe Ile His Val Gly Cys Pro His Tyr Trp Arg Phe Gly Gln Ala 
            180                 185                 190         


Gly Glu Thr Glu Ala Glu Phe Thr Gln Arg Leu Ala Arg Glu Leu Glu 
        195                 200                 205             


Ala Thr Ile Ile Lys Glu Gly Pro Asp Thr Ile Ala Gly Phe Phe Ala 
    210                 215                 220                 


Glu Pro Val Met Gly Ala Gly Gly Val Ile Pro Pro Ser Glu Gly Tyr 
225                 230                 235                 240 


Phe Gln Ala Val Gln Pro Val Leu Lys Arg Tyr Gly Ile Pro Leu Ile 
                245                 250                 255     


Ala Asp Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly 
            260                 265                 270         


Cys Gln Thr Tyr Asp Phe Met Pro Asp Gly Ile Ile Ser Ser Lys Asn 
        275                 280                 285             


Ile Thr Ala Gly Phe Phe Pro Met Gly Ala Val Ile Leu Gly Pro Glu 
    290                 295                 300                 


Leu Ala Asp Arg Leu Gln Ala Ala Ser Glu Ala Val Glu Glu Phe Pro 
305                 310                 315                 320 


His Gly Phe Thr Ala Ser Gly His Pro Val Gly Cys Ala Ile Ala Leu 
                325                 330                 335     


Lys Ala Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg 
            340                 345                 350         


Ala Leu Thr Pro Lys Phe Glu Ala Gly Leu Ala Tyr Leu Ala Glu Asn 
        355                 360                 365             


Pro Asn Ile Gly Glu Trp Arg Gly Lys Gly Leu Met Gly Ala Leu Glu 
    370                 375                 380                 


Ala Val Lys Asp Lys Ala Thr Lys Thr Pro Phe Pro Gly Asp Leu Ser 
385                 390                 395                 400 


Val Ser Glu Arg Ile Ala Asn Ser Cys Thr Asp His Gly Leu Ile Cys 
                405                 410                 415     


Arg Pro Leu Gly Gln Ser Ile Val Leu Cys Pro Pro Phe Ile Met Thr 
            420                 425                 430         


Glu Ala Gln Met Asp Glu Met Phe Glu Lys Leu Gly Ala Ala Leu Lys 
        435                 440                 445             


Lys Val Phe Ala Glu Val Ala 
    450                 455 


<210>  11
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  11
gccatcatag gggttcctaa aga                                               23


<210>  12
<211>  30
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  12
actgatggta ccttaagcac ccgccacaga                                        30


<210>  13
<211>  33
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  13
gtgacgatca ttgcccgtaa cgcagaccgc ttg                                    33


<210>  14
<211>  33
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  14
caagcggtct gcgttacggg caatgatcgt cac                                    33


<210>  15
<211>  27
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  15
gcctatcgac tattaaacaa aacagcc                                           27


<210>  16
<211>  30
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  16
actgatggta ccttagacct gggtcaatcc                                        30


<210>  17
<211>  1401
<212>  DNA
<213>  Paracoccus denitrificans

<400>  17
atggctagca gaggatcgca tcaccatcac catcacggcg ccaaccaacc gcaaagctgg       60

gaagcccggg ccgagaccta ttcgctctac ggtttcaccg acatgccctc ggtccatcag      120

cggggcacgg tcgtcgtgac ccatggcgag gggccctata tcgtcgatgt ccatggccgc      180

cgctatctgg atgccaattc gggcctgtgg aacatggtcg cgggcttcga ccacaagggc      240

ctgatcgagg ccgccaaggc gcaatacgac cgctttcccg gctatcacgc ctttttcggc      300

cgcatgtccg accagaccgt gatgctgtcg gaaaagctgg tcgaggtctc gccattcgac      360

aacggccggg tcttctatac caattccggc tccgaggcga acgacaccat ggtcaagatg      420

ctgtggttcc tgcatgccgc cgagggcaag ccgcaaaagc gcaagatcct gacgcgctgg      480

aacgcctatc acggcgtgac cgcggtttcg gcctcgatga ccggcaagcc ctacaactcg      540

gtcttcggcc tgccgctgcc cggcttcatc cacctgacct gcccgcatta ctggcgctat      600

ggcgaggaag gcgagaccga ggcgcaattc gtcgcccgcc tggcacgcga gcttgaggat      660

accatcaccc gcgagggcgc cgacaccatc gccggcttct tcgccgagcc ggtgatgggc      720

gcgggggggg tgatcccgcc ggcgaagggt tatttccagg ccatcctgcc gatcttgcgc      780

aagtatgaca tcccgatgat ctcggacgag gtgatctgcg gcttcgggcg caccggcaac      840

acctggggct gcctgaccta cgacttcatg cccgatgcga tcatctcgtc caagaacctg      900

actgcgggct tcttcccgat gggcgccgtc atcctcgggc ccgacctcgc caagcgggtc      960

gaggccgcgg tcgaggcgat cgaggagttc ccgcacggct tcaccgcctc gggccatccg     1020

gtcggctgcg ccatcgcgct gaaggccatc gacgtggtga tgaacgaggg gctggccgag     1080

aatgtccgcc gcctcgcacc ccgcttcgag gcggggctga agcgcatcgc cgaccgcccg     1140

aacatcggcg aataccgcgg catcggcttc atgtgggcgc tggaggcggt caaggacaag     1200

ccgaccaaga cccccttcga cgccaatctt tcggtcagcg agcgcatcgc caatacctgc     1260

accgatctgg ggctgatctg ccggccgctg ggccagtcca tcgtgctgtg cccgcccttc     1320

atcctgaccg aggcgcagat ggacgagatg ttcgaaaagc tggaaaaggc gctcgacaag     1380

gtctttgccg aggtggccta a                                               1401


<210>  18
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  18
gccaaccaac cgcaaagctg gga                                               23


<210>  19
<211>  33
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  19
actgataagc ttttaggcca cctcggcaaa gac                                    33


<210>  20
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  20
ctgccggcca atgggccagt ccat                                              24


<210>  21
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  primer

<400>  21
atggactggc ccattggccg gcag                                              24


<210>  22
<211>  391
<212>  PRT
<213>  Artificial

<220>
<223>  Bacillus subitilis protein mit His-Tag

<400>  22

Met Ala Ser Arg Gly Ser His His His His His His Gly Ala Ile Ile 
1               5                   10                  15      


Gly Val Pro Lys Glu Ile Lys Asn Asn Glu Asn Arg Val Ala Leu Thr 
            20                  25                  30          


Pro Gly Gly Val Ser Gln Leu Ile Ser Asn Gly His Arg Val Leu Val 
        35                  40                  45              


Glu Thr Gly Ala Gly Leu Gly Ser Gly Phe Glu Asn Glu Ala Tyr Glu 
    50                  55                  60                  


Ser Ala Gly Ala Glu Ile Ile Ala Asp Pro Lys Gln Val Trp Asp Ala 
65                  70                  75                  80  


Glu Met Val Met Lys Val Lys Glu Pro Leu Pro Glu Glu Tyr Val Tyr 
                85                  90                  95      


Phe Arg Lys Gly Leu Val Leu Phe Thr Tyr Leu His Leu Ala Ala Glu 
            100                 105                 110         


Pro Glu Leu Ala Gln Ala Leu Lys Asp Lys Gly Val Thr Ala Ile Ala 
        115                 120                 125             


Tyr Glu Thr Val Ser Glu Gly Arg Thr Leu Pro Leu Leu Thr Pro Met 
    130                 135                 140                 


Ser Glu Val Ala Gly Arg Met Ala Ala Gln Ile Gly Ala Gln Phe Leu 
145                 150                 155                 160 


Glu Lys Pro Lys Gly Gly Lys Gly Ile Leu Leu Ala Gly Val Pro Gly 
                165                 170                 175     


Val Ser Arg Gly Lys Val Thr Ile Ile Gly Gly Gly Val Val Gly Thr 
            180                 185                 190         


Asn Ala Ala Lys Met Ala Val Gly Leu Gly Ala Asp Val Thr Ile Ile 
        195                 200                 205             


Ala Arg Asn Ala Asp Arg Leu Arg Gln Leu Asp Asp Ile Phe Gly His 
    210                 215                 220                 


Gln Ile Lys Thr Leu Ile Ser Asn Pro Val Asn Ile Ala Asp Ala Val 
225                 230                 235                 240 


Ala Glu Ala Asp Leu Leu Ile Cys Ala Val Leu Ile Pro Gly Ala Lys 
                245                 250                 255     


Ala Pro Thr Leu Val Thr Glu Glu Met Val Lys Gln Met Lys Pro Gly 
            260                 265                 270         


Ser Val Ile Val Asp Val Ala Ile Asp Gln Gly Gly Ile Val Glu Thr 
        275                 280                 285             


Val Asp His Ile Thr Thr His Asp Gln Pro Thr Tyr Glu Lys His Gly 
    290                 295                 300                 


Val Val His Tyr Ala Val Ala Asn Met Pro Gly Ala Val Pro Arg Thr 
305                 310                 315                 320 


Ser Thr Ile Ala Leu Thr Asn Val Thr Val Pro Tyr Ala Leu Gln Ile 
                325                 330                 335     


Ala Asn Lys Gly Ala Val Lys Ala Leu Ala Asp Asn Thr Ala Leu Arg 
            340                 345                 350         


Ala Gly Leu Asn Thr Ala Asn Gly His Val Thr Tyr Glu Ala Val Ala 
        355                 360                 365             


Arg Asp Leu Gly Tyr Glu Tyr Val Pro Ala Glu Lys Ala Leu Gln Asp 
    370                 375                 380                 


Glu Ser Ser Val Ala Gly Ala 
385                 390     


<210>  23
<211>  1176
<212>  DNA
<213>  artificial

<220>
<223>  Bacillus subtilis protein mit His-Tag

<400>  23
atggctagca gaggatcgca tcaccatcac catcacggcg ccatcatagg ggttcctaaa       60

gagataaaaa acaatgaaaa ccgtgtcgca ttaacacccg ggggcgtttc tcagctcatt      120

tcaaacggcc accgggtgct ggttgaaaca ggcgcgggcc ttggaagcgg atttgaaaat      180

gaagcctatg agtcagcagg agcggaaatc attgctgatc cgaagcaggt ctgggacgcc      240

gaaatggtca tgaaagtaaa agaaccgctg ccggaagaat atgtttattt tcgcaaagga      300

cttgtgctgt ttacgtacct tcatttagca gctgagcctg agcttgcaca ggccttgaag      360

gataaaggag taactgccat cgcatatgaa acggtcagtg aaggccggac attgcctctt      420

ctgacgccaa tgtcagaggt tgcgggcaga atggcagcgc aaatcggcgc tcaattctta      480

gaaaagccta aaggcggaaa aggcattctg cttgccgggg tgcctggcgt ttcccgcgga      540

aaagtaacaa ttatcggagg aggcgttgtc gggacaaacg cggcgaaaat ggctgtcggc      600

ctcggtgcag atgtgacgat cattgcccgt aacgcagacc gcttgcgcca gcttgatgac      660

atcttcggcc atcagattaa aacgttaatt tctaatccgg tcaatattgc tgatgctgtg      720

gcggaagcgg atctcctcat ttgcgcggta ttaattccgg gtgctaaagc tccgactctt      780

gtcactgagg aaatggtaaa acaaatgaaa cccggttcag ttattgttga tgtagcgatc      840

gaccaaggcg gcatcgtcga aactgtcgac catatcacaa cacatgatca gccaacatat      900

gaaaaacacg gggttgtgca ttatgctgta gcgaacatgc caggcgcagt ccctcgtaca      960

tcaacaatcg ccctgactaa cgttactgtt ccatacgcgc tgcaaatcgc gaacaaaggg     1020

gcagtaaaag cgctcgcaga caatacggca ctgagagcgg gtttaaacac cgcaaacgga     1080

cacgtgacct atgaagctgt agcaagagat ctaggctatg agtatgttcc tgccgagaaa     1140

gctttacagg atgaatcatc tgtggcgggt gcttaa                               1176


