                         SEQUENCE LISTING

<110>  Lin, Pei-Chun
       Rubin, Mark A.
 
<120>  MiRNA-31 as a Diagnostic, Prognostic and Therapeutic Agent in 
       Cancer

<130>  28710

<150>  61/623,266
<151>  2012-04-12

<160>  109   

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  microRNA-31 nucleotide sequence

<400>  1
ucgauacggu cguagaacgg a                                                 21


<210>  2
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for amplifying CpG island 1 in the miR-31 promoter
       region

<400>  2
ttttttttaa gaagggaaag tttag                                             25


<210>  3
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for amplifying CpG island 1 in the miR-31 promoter
       region

<400>  3
caaataaact aaaaaaacct taatccc                                           27


<210>  4
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for amplifying CpG island 2 in the miR-31 promoter
       region

<400>  4
tttttaggag gagtttggtg agtag                                             25


<210>  5
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for amplifying CpG island 2 in the miR-31 promoter
       region

<400>  5
aacctcctca actccttaaa a                                                 21


<210>  6
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for amplifying CpG island 3 in the miR-31 promoter
       region

<400>  6
gttgtagtgg agaaatttgg gttt                                              24


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for amplifying CpG island 3 in the miR-31 promoter
       region

<400>  7
caccaaactc ctcctaaaaa                                                   20


<210>  8
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for amplifying CpG island 4 in the miR-31 promoter
       region

<400>  8
tggtttttgt aggtggattt tttt                                              24


<210>  9
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for amplifying CpG island 4 in the miR-31 promoter
       region

<400>  9
tactaaacct cctcccttaa accc                                              24


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for PCR of MIR31HG promoter region

<400>  10
ccccaagtta tgcacaggtc                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for PCR of MIR31HG promoter region

<400>  11
cccttcaaat ccaggtgaaa                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for PCR of MIR31HG promoter region

<400>  12
taaagcagct gcccaatttt                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for PCR of MIR31HG promoter region

<400>  13
cgaagtcaca ggttcgctct                                                   20


<210>  14
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 promoter sequence for binding of T7 polymerase during 
       transcription and cloning into vectors

<400>  14
cagtaatacg actcactata gggagaaggc t                                      31


<210>  15
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Therapeutic nucleic acid corresponding to a portion of miR-31 
       that binds AR


<220>
<221>  misc_feature
<222>  (4)..(6)
<223>  N being independently AUCG

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  N being independently AUCG

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  N being independently AUCG

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  N being independently AUCG

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  N being independently AUCG

<400>  15
ucgnnncngu cnuagaacng n                                                 21


<210>  16
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Therapeutic nucleic acid corresponding to a portion of miR-31 
       that binds AR


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  N being independently AUCG

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  N being independently AUCG

<220>
<221>  misc_feature
<222>  (11)..(12)
<223>  N being independently AUCG

<220>
<221>  misc_feature
<222>  (15)..(16)
<223>  N being independently AUCG

<400>  16
ucnanncggu nncgnngaac gga                                               23


<210>  17
<211>  27
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Therapeutic nucleic acid corresponding to a portion of miR-31 
       that binds AR


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  N being independently AUCG

<220>
<221>  misc_feature
<222>  (12)..(19)
<223>  N being independently AUCG

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  N being independently AUCG

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  N being independently AUCG

<400>  17
nnnnnncggu cnnnnnnnna naacggn                                           27


<210>  18
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for miR-31 CpG region 3

<400>  18
aggaagagag gttgtagtgg agaaatttgg gttt                                   34


<210>  19
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for miR-31 CpG region 3

<400>  19
cagtaatacg actcactata gggagaaggc tctcaccaaa ctcctcctaa aaa              53


<210>  20
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for miR-31 CpG region 4

<400>  20
aggaagagag tggtttttgt aggtggattt tttt                                   34


<210>  21
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for miR-31 CpG region 4

<400>  21
cagtaatacg actcactata gggagaaggc ttactaaacc tcctccctta aaccc            55


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for PCR of MIR31HG mRNA

<400>  22
cgcttctgtc ctcctactcg                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for PCR of MIR31HG mRNA

<400>  23
acaagcagac ccttggaatg                                                   20


<210>  24
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for PCR of PSA mRNA

<400>  24
tgtgtgctgg acgctgga                                                     18


<210>  25
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for PCR of PSA mRNA

<400>  25
cactgcccca tgacgtgat                                                    19


<210>  26
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for PCR of TMPRSS2 mRNA

<400>  26
ggacagtgtg cacctcaaag ac                                                22


<210>  27
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for PCR of TMPRSS2 mRNA

<400>  27
tcccacgagg aaggtccc                                                     18


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for PCR of NDRG1 mRNA

<400>  28
gtggagaaag gggagaccat                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for PCR of NDRG1 mRNA

<400>  29
acagcgtgac gtgaacagag                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for PCR of HMBS mRNA

<400>  30
ccatcatcct ggcaacagct                                                   20


<210>  31
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for PCR of HMBS mRNA

<400>  31
gcattcctca gggtgcagg                                                    19


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for miR-31 promoter region 1000 nucleotides 
       downstream of miR-31HG gene start site

<400>  32
ccgatgacct agccagaagt                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for miR-31 promoter region 1000 nucleotides 
       downstream of miR-31HG gene start site

<400>  33
ccccaccctt caactcgtag                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for miR-31 promoter region 500 nucleotides 
       downstream of miR-31HG gene start site

<400>  34
tatcctcaac cctccgtgtc                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for miR-31 promoter region 500 nucleotides 
       downstream of miR-31HG gene start site

<400>  35
catacacctg aaggggcagt                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for miR-31 promoter region 1000 nucleotides 
       upstream of miR-31HG gene start site

<400>  36
caattttggc ccaggagata                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for miR-31 promoter region 500 nucleotides 
       upstream of miR-31HG gene start site

<400>  37
tttccgggga cctctagttt                                                   20


<210>  38
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for miR-31 promoter region 42,500 nucleotides 
       upstream of miR-31HG gene start site

<400>  38
tggcctattt gctgttctaa tgac                                              24


<210>  39
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for miR-31 promoter region 42,500 nucleotides 
       upstream of miR-31HG gene start site

<400>  39
gcaagccaac cccaaca                                                      17


<210>  40
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for miR-31 promoter region 45,000 nucleotides 
       upstream of miR-31HG gene start site

<400>  40
aatgggccct gcattctct                                                    19


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for miR-31 promoter region 45000 nucleotides 
       upstream of miR-31HG gene start site

<400>  41
aaaacccaca ccctcaccac                                                   20


<210>  42
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for miR-31 promoter region 47,500 nucleotides 
       upstream of miR-31HG gene start site

<400>  42
catcttcaaa agcggacact ct                                                22


<210>  43
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for miR-31 promoter region 47,500 nucleotides 
       upstream of miR-31HG gene start site

<400>  43
acaatacata gcaggacagg aag                                               23


<210>  44
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for PSA gene start site

<400>  44
cctagatgaa gtctccatga gctaca                                            26


<210>  45
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for PSA gene start site

<400>  45
gggagggaga gctagcactt g                                                 21


<210>  46
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for MYT1 gene start site

<400>  46
aggcaccttc tgttggccga                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for MYT1 gene start site

<400>  47
aggcagctgc ctcccgtaca                                                   20


<210>  48
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for GAPDH gene start site

<400>  48
cggctactag cggttttacg                                                   20


<210>  49
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for GAPDH gene start site

<400>  49
aagaagatgc ggctgactgt                                                   20


<210>  50
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of AR microRNA 
       recognition element 1

<400>  50
tcgagctagc agggcagatc ttgtccgc                                          28


<210>  51
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of AR microRNA 
       recognition element 1

<400>  51
ggccgcggac aagatctgcc ctgctagc                                          28


<210>  52
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of AR microRNA 
       recognition element 2

<400>  52
tcgagaagtg ggccaaggcc ttgcctgc                                          28


<210>  53
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of AR microRNA 
       recognition element 2

<400>  53
ggccgcaggc aaggccttgg cccacttc                                          28


<210>  54
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of AR microRNA 
       recognition element 3

<400>  54
tcgaggccag ggaccatgtt ttgcccgc                                          28


<210>  55
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of AR microRNA 
       recognition element 3

<400>  55
ggccgcgggc aaaacatggt ccctggcc                                          28


<210>  56
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of AR microRNA 
       recognition element 4

<400>  56
tcgagatctg ttccatcttc ttgcctgc                                          28


<210>  57
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of AR microRNA 
       recognition element 4

<400>  57
ggccgcaggc aagaagatgg aacagatc                                          28


<210>  58
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the AR microRNA 
       recognition element 2 mutant (mt)

<400>  58
tcgagaagtg ggccaaggcc aagggtgc                                          28


<210>  59
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the AR microRNA 
       recognition element 2 mutant (mt)

<400>  59
ggccgcaccc ttggccttgg cccacttc                                          28


<210>  60
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the AR microRNA 
       recognition element 2 mutant (mt1)

<400>  60
tcgagaagtg ggccgaggcc ttgcctgc                                          28


<210>  61
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the AR microRNA 
       recognition element 2 mutant (mt1)

<400>  61
ggccgcaggc aaggcctcgg cccacttc                                          28


<210>  62
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the AR microRNA 
       recognition element 2 mutant (mt2)

<400>  62
tcgagaagtg ggccaagacc ttgcctgc                                          28


<210>  63
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the AR microRNA 
       recognition element 2 mutant (mt2)

<400>  63
ggccgcaggc aaggtcttgg cccacttc                                          28


<210>  64
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the AR microRNA 
       recognition element 2 mutant (mt3)

<400>  64
tcgagaagtg ggccaaggcc tttcctgc                                          28


<210>  65
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the AR microRNA 
       recognition element 2 mutant (mt3)

<400>  65
ggccgcagga aaggccttgg cccacttc                                          28


<210>  66
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the AR microRNA 
       recognition element 3 mutant (mt4)

<400>  66
tcgaggccag ggaccatgtt ttcccgc                                           27


<210>  67
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the AR microRNA 
       recognition element 3 mutant (mt4)

<400>  67
ggccgcggga aaacatggtc cctggcc                                           27


<210>  68
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the AR microRNA 
       recognition element 4 mutant (mt)

<400>  68
tcgagatctg ttccatcttg aagggtgc                                          28


<210>  69
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the AR microRNA 
       recognition element 4 mutant (mt)

<400>  69
ggccgcaccc aacaagatgg aacagatc                                          28


<210>  70
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the CDK1 3' UTR 
       domain

<400>  70
atgactcgag acgaatttct ggcaaaatgg                                        30


<210>  71
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the CDK1 3' UTR 
       domain

<400>  71
atgagcggcc gcgagccttt ttagatggct gct                                    33


<210>  72
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the E2F2 3'UTR domain

<400>  72
atgactcgag ccccctctac tgtccctttc                                        30


<210>  73
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the E2F2 3'UTR domain

<400>  73
atgagcggcc gcggaccatg ttctctcggt tc                                     32


<210>  74
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the EXO1 3'UTR domain

<400>  74
atgactcgag tccagaagcg gaagaggata                                        30


<210>  75
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the EXO1 3'UTR domain

<400>  75
atgagcggcc gccctccaaa aacctgtcag aga                                    33


<210>  76
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the FOXM1 3'UTR 
       domain

<400>  76
atgactcgag ccctgacaac atcaactggt c                                      31


<210>  77
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the FOXM1 3'UTR 
       domain

<400>  77
atgagcggcc gctttttgtc caccttcgct tt                                     32


<210>  78
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the MCM2 3'UTR domain

<400>  78
atgactcgag ttccttggga ttctggtttg                                        30


<210>  79
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the MCM2 3'UTR domain

<400>  79
atgagcggcc gccagcctga atacgcaact ca                                     32


<210>  80
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the AR long 3' UTR 
       domain

<400>  80
atgactcgag tggagccaga ggagaagaaa                                        30


<210>  81
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the AR long 3' UTR 
       domain

<400>  81
atgagcggcc gcgagttcat gggtggcaaa gt                                     32


<210>  82
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the CDK1 microRNA 
       recognition element

<400>  82
cgagagagca tgccaaaatt tgctaagc                                          28


<210>  83
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the CDK1 microRNA 
       recognition element

<400>  83
ggccgcttag caaattttgg catgctctc                                         29


<210>  84
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the E2F2 microRNA 
       recognition element 1

<400>  84
tcgagagctc atgcccccgt cttgccagc                                         29


<210>  85
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the E2F2 microRNA 
       recognition element 1

<400>  85
ggccgctggc aagacggggg catgagctc                                         29


<210>  86
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the E2F2 microRNA 
       recognition element 1 mutant

<400>  86
tcgagacgac tacggtcgta gaacggagc                                         29


<210>  87
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the E2F2 microRNA 
       recognition element 1 mutant

<400>  87
ggccgctccg ttctacgacc gtagtcgtc                                         29


<210>  88
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the E2F2 microRNA 
       recognition element 2

<400>  88
tcgaggtgag ctgaagaacc ttgcctgc                                          28


<210>  89
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the E2F2 microRNA 
       recognition element 2

<400>  89
ggccgcaggc aaggttcttc agctcacc                                          28


<210>  90
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the E2F2 microRNA 
       recognition element 3

<400>  90
tcgaggcagc tgtgggccct tttgccgc                                          28


<210>  91
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the E2F2 microRNA 
       recognition element 3

<400>  91
ggccgcggca aaagggccca cagctgcc                                          28


<210>  92
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the FOXM1 microRNA 
       recognition element

<400>  92
tcgaggacaa gtggatctgc ttgccaggc                                         29


<210>  93
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the FOXM1 microRNA 
       recognition element

<400>  93
ggccgcctgg caagcagatc cacttgtcc                                         29


<210>  94
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the FOXM1 microRNA 
       recognition element mutant

<400>  94
tcgaggagat gtggttgtgg aacggaggc                                         29


<210>  95
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the FOXM1 microRNA 
       recognition element mutant

<400>  95
ggccgcctcc gttccacaac cacatctcc                                         29


<210>  96
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the MCM2 microRNA 
       recognition element

<400>  96
tcgagtggtt gctgaacatc ttgccagc                                          28


<210>  97
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the MCM2 microRNA 
       recognition element

<400>  97
ggccgctggc aagatgttca gcaaccac                                          28


<210>  98
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for construct preparation of the MCM2 microRNA 
       recognition element mutant

<400>  98
tcgagtggtt gctgaacatc aagggagc                                          28


<210>  99
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for construct preparation of the MCM2 microRNA 
       recognition element mutant

<400>  99
ggccgctccc ttgatgttca gcaaccac                                          28


<210>  100
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for site directed mutagenesis of the AR long 3'UTR
       mutant

<400>  100
ggcaagctgg gcgtcttaaa cttgtccccc agagatg                                37


<210>  101
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for site directed mutagenesis of the AR long 3'UTR
       mutant

<400>  101
catctctggg ggacaagttt aagacgccca gcttgcc                                37


<210>  102
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for site directed mutagenesis of the AR CDS mutant

<400>  102
caagtgggcc aaggcctttc ctggcttccg caacttac                               38


<210>  103
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for site directed mutagenesis of the AR CDS mutant

<400>  103
gtaagttgcg gaagccagga aaggccttgg cccacttg                               38


<210>  104
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for site directed mutagenesis of the E2F1 3'UTR 
       mutant

<400>  104
gtttccagag atgctcacca actctctgca gccctggagc                             40


<210>  105
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for site directed mutagenesis of the E2F1 3'UTR 
       mutant

<400>  105
gctccagggc tgcagagagt tggtgagcat ctctggaaac                             40


<210>  106
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for miR-31 CpG region1

<400>  106
aggaagagag ttttttttaa gaagggaaag tttag                                  35


<210>  107
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for miR-31 CpG region1

<400>  107
cagtaatacg actcactata gggagaaggc tcaaataaac taaaaaaacc ttaatccc         58


<210>  108
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for miR-31 CpG region 2

<400>  108
aggaagagag tttttaggag gagtttggtg agtag                                  35


<210>  109
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for miR-31 CpG region 2

<400>  109
cagtaatacg actcactata gggagaaggc taaacctcct caactcctta aaa              53


