               SEQUENCE LISTING

<110> Etablissement Français du Sang (EFS)
      Centre National de la Recherche Scientifique (CNRS)
      Université de Montpellier 1
      Université Claude Bernard Lyon 1

<120> Oligonucleotides modifies comprenant des fonctions thiol et leur utilisation pour la detection d'acides nucleiques

<130> 33740 EFN

<160> 44

<170> BiSSAP 1.0

<210> 1
<211> 15
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..15
<223> /mol_type="DNA"
      /note="Sonde VHC 1a/1b"
      /organism="artificial sequences"

<400> 1
gtgcagtccy ggagc                                                     15


<210> 2
<211> 15
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..15
<223> /mol_type="DNA"
      /note="Cible VHC 1a/1b 15-mères"
      /organism="artificial sequences"

<400> 2
gctccrggac tgcac                                                     15


<210> 3
<211> 105
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..105
<223> /mol_type="DNA"
      /note="Cible VHC 1a/1b 105-mères"
      /organism="artificial sequences"

<400> 3
cctcacttgc tacatcaagg cccaggcagc ctgtcgagcc gcagggctcc gggactgcac     60

catgctcgtg tgtggcgacg acttagtcgt tatctgtgaa agtgc                    105


<210> 4
<211> 15
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..15
<223> /mol_type="DNA"
      /note="Sonde VHC 3a"
      /organism="artificial sequences"

<400> 4
ggcctccggt tcaag                                                     15


<210> 5
<211> 15
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..15
<223> /mol_type="DNA"
      /note="Cible VHC 3a 15-mères"
      /organism="artificial sequences"

<400> 5
cttgaaccgg aggcc                                                     15


<210> 6
<211> 105
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..105
<223> /mol_type="DNA"
      /note="Cible VHC 3a 105-mères"
      /organism="artificial sequences"

<400> 6
gaacaggaca tcagggtgga agaggagata taccaatgct gtaaccttga accggaggcc     60

aggaaagtga tctcctccct cacggagcgg ctttactgcg ggggc                    105


<210> 7
<211> 13
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..13
<223> /mol_type="DNA"
      /note="Acide nucléique test greffage"
      /organism="artificial sequences"

<400> 7
ccaagcacga tgt                                                       13


<210> 8
<211> 31
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..31
<223> /mol_type="DNA"
      /note="Amorce VHC sens biotinylée"
      /organism="artificial sequences"

<400> 8
tggggatccc gtatgatacc cgctgctttg a                                   31


<210> 9
<211> 30
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..30
<223> /mol_type="DNA"
      /note="Amorce VHC anti-sens"
      /organism="artificial sequences"

<400> 9
ggcggaattc ctggtcatag cctccgtgaa                                     30


<210> 10
<211> 25
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..25
<223> /mol_type="DNA"
      /note="Amorce VHC 1a/1b sens biotinylée"
      /organism="artificial sequences"

<400> 10
tgacracyag ctgyggtaay accct                                          25


<210> 11
<211> 21
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..21
<223> /mol_type="DNA"
      /note="Amorce VHC anti-sens 3a rev "
      /organism="artificial sequences"

<400> 11
gcagtaaagc cgytccgtga g                                              21


<210> 12
<211> 401
<212> DNA
<213> Hepatitis C virus

<220> 
<221> source
<222> 1..401
<223> /mol_type="DNA"
      /note="Amplicon VHC long 401nt 1a/1b #PTR6719"
      /organism="Hepatitis C virus"

<400> 12
tggggatccc gtatgatacc cgctgctttg actcaacggt cactgagaat gacatccgtg      60

ttgaggagtc aatttaccaa tgttgtgacc tagcccccga agccagacag gccataaggt     120

cgctcacaga gcggctttac atcgggggtc ccctgactaa ttcaaaaggg cagaactgcg     180

gctatcgccg gtgccgcgcg agcggtgtgc tgacgaccag ctgcggtaat accctcacat     240

gttacttgaa ggcctctgcg gcctgtcgag ctgcaaagct ccaggactgc acaatgctcg     300

tgtgcggaga cgaccttgtc gttatctgtg aaagcgcggg aacccargag gatgcggcga     360

gcctacgagt cttcacggag gctatgacca ggaattccgc c                         401


<210> 13
<211> 191
<212> DNA
<213> Hepatitis C virus

<220> 
<221> source
<222> 1..191
<223> /mol_type="DNA"
      /note="Amplicon VHC court 191nt 1a/1b #PTR6719"
      /organism="Hepatitis C virus"

<400> 13
tgacgaccag ctgcggtaat accctcacat gttacttgaa ggcctctgcg gcctgtcgag     60

ctgcaaagct ccaggactgc acaatgctcg tgtgcggaga cgaccttgtc gttatctgtg    120

aaagcgcggg aacccargag gatgcggcga gcctacgagt cttcacggag gctatgacca    180

ggaattccgc c                                                         191


<210> 14
<211> 401
<212> DNA
<213> Hepatitis C virus

<220> 
<221> source
<222> 1..401
<223> /mol_type="DNA"
      /note="Amplicon VHC long 401nt 3a #PTR9058"
      /organism="Hepatitis C virus"

<400> 14
tggggatccc gtatgatacc cgctgctttg actcgactgt cactgaacag gatatcaggg      60

tggaagagga gatataccaa tgctgtaatc ttgaaccgga ggccaggaag gtgatctcct     120

ccctcacgga gcggctttac tgcgggggtc ctatgttcaa cagcaaaggg gcccagtgtg     180

gttatcgccg ttgccgtgct agtggagttc tacctaccag cttcggcaat acaatcactt     240

gctacatcaa ggccacagcg gctgcaaggg ccgcaggcct ccggaacccg gactttcttg     300

tctgcggaga cgatctagtc gtggtggctg agagtgacgg cgtcgacgag gatggggcgg     360

ccctgagagc cttcacggag gctatgacca ggaattccgc c                         401


<210> 15
<211> 143
<212> DNA
<213> Hepatitis C virus

<220> 
<221> source
<222> 1..143
<223> /mol_type="DNA"
      /note="Amplicon VHC court 143nt 3a #PTR9058"
      /organism="Hepatitis C virus"

<400> 15
tggggatccc gtatgatacc cgctgctttg actcgactgt cactgaacag gatatcaggg     60

tggaagagga gatataccaa tgctgtaatc ttgaaccgga ggccaggaag gtgatctcct    120

ccctcacgga gcggctttac tgc                                            143


<210> 16
<211> 14
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..14
<223> /mol_type="DNA"
      /note="Sonde générique 1 flavivirus"
      /organism="artificial sequences"

<400> 16
gctcccarcc acat                                                      14


<210> 17
<211> 14
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..14
<223> /mol_type="DNA"
      /note="Sonde générique 2 flavivirus"
      /organism="artificial sequences"

<400> 17
aaccatctrt cttc                                                      14


<210> 18
<211> 15
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..15
<223> /mol_type="DNA"
      /note="Sonde spécifique 3 Dengue"
      /organism="artificial sequences"

<400> 18
cttcyccttc yactc                                                     15


<210> 19
<211> 15
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..15
<223> /mol_type="DNA"
      /note="Sonde spécifique 4 West Nile "
      /organism="artificial sequences"

<400> 19
ckcctcctga rttct                                                     15


<210> 20
<211> 23
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..23
<223> /mol_type="DNA"
      /note="Amorce sens flavivirus MAMD "
      /organism="artificial sequences"

<400> 20
aacatgatgg graaragrga raa                                            23


<210> 21
<211> 26
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..26
<223> /mol_type="DNA"
      /note="Amorce anti-sens flavivirus cFD2"
      /organism="artificial sequences"

<400> 21
gtgtcccagc cggcggtgtc atcagc                                         26


<210> 22
<211> 22
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..22
<223> /mol_type="DNA"
      /note="Amorce sens flavivirus FS778"
      /organism="artificial sequences"

<400> 22
aargghagym cdgchathtg gt                                             22


<210> 23
<211> 263
<212> DNA
<213> Dengue virus 1

<220> 
<221> source
<222> 1..263
<223> /mol_type="DNA"
      /note="Amplicon Dengue 1 263nt FJ882517"
      /organism="Dengue virus 1"

<400> 23
aacatgatgg ggaagagaga gaaaaaacta ggagagttcg gaaaggcaaa aggaagtcgt     60

gcaatatggt acatgtggct gggagcacgc tttctagagt tcgaagctct tggtttcatg    120

aacgaagatc actggttcag cagagagaat tcactcagcg gagtggaagg agaaggactc    180

cacaaacttg gatatatact cagagacata tcaaagattc cagggggaaa catgtatgca    240

gatgacacag ccggatggga cac                                            263


<210> 24
<211> 215
<212> DNA
<213> Dengue virus 1

<220> 
<221> source
<222> 1..215
<223> /mol_type="DNA"
      /note="Amplicon Dengue 1 215nt FJ882517"
      /organism="Dengue virus 1"

<400> 24
aaaggaagtc gtgcaatatg gtacatgtgg ctgggagcac gctttctaga gttcgaagct     60

cttggtttca tgaacgaaga tcactggttc agcagagaga attcactcag cggagtggaa    120

ggagaaggac tccacaaact tggatatata ctcagagaca tatcaaagat tccaggggga    180

aacatgtatg cagatgacac agccggatgg gacac                               215


<210> 25
<211> 263
<212> DNA
<213> West Nile virus

<220> 
<221> source
<222> 1..263
<223> /mol_type="DNA"
      /note="Amplicon WNV 263nt EF429200H442"
      /organism="West Nile virus"

<400> 25
aacatgatgg gaaagagaga gaagaagcct ggagagttcg gcaaggctaa aggcagcaga     60

gccatctggt tcatgtggct gggggctcgt ttcctggagt ttgaagctct cggattcctc    120

aatgaagacc actggctggg taggaagaac tcaggaggag gagttgaagg cttaggactg    180

cagaagcttg ggtacatctt gaaggaagtt gggacaaagc ctggaggaaa gatttacgcc    240

gatgataccg caggctggga cac                                            263


<210> 26
<211> 215
<212> DNA
<213> West Nile virus

<220> 
<221> source
<222> 1..215
<223> /mol_type="DNA"
      /note="Amplicon WNV 215nt EF429200H442"
      /organism="West Nile virus"

<400> 26
aaaggcagca gagccatctg gttcatgtgg ctgggggctc gtttcctgga gtttgaagct     60

ctcggattcc tcaatgaaga ccactggctg ggtaggaaga actcaggagg aggagttgaa    120

ggcttaggac tgcagaagct tgggtacatc ttgaaggaag ttgggacaaa gcctggagga    180

aagatttacg ccgatgatac cgcaggctgg gacac                               215


<210> 27
<211> 15
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..15
<223> /mol_type="DNA"
      /note="Sonde VHC 2"
      /organism="artificial sequences"

<400> 27
tggctytctg agatg                                                     15


<210> 28
<211> 15
<212> DNA
<213> Hepatitis C virus

<220> 
<221> source
<222> 1..15
<223> /mol_type="DNA"
      /note="Sonde VHC 4a/4d"
      /organism="Hepatitis C virus"

<400> 28
ggrccgccca crtag                                                     15


<210> 29
<211> 21
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..21
<223> /mol_type="DNA"
      /note="Amorce VHC2 sens biotinylée"
      /organism="artificial sequences"

<400> 29
atgytggtrt gcggcgacga c                                              21


<210> 30
<211> 23
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..23
<223> /mol_type="DNA"
      /note="Amorce VHC anti-sens 4 rev"
      /organism="artificial sequences"

<400> 30
aggtctcccy tgctgttgtr cat                                            23


<210> 31
<211> 401
<212> DNA
<213> Hepatitis C virus

<220> 
<221> source
<222> 1..401
<223> /mol_type="DNA"
      /note="AmpliconVHC long 2 401nt #PTR7761"
      /organism="Hepatitis C virus"

<400> 31
tggggatccc gtatgatacc cgctgctttg actcaactgt cactgagaga gacatcagaa      60

ccgaggagtc catataccag gcctgctccc taaccgagga ggctcgcacc gccatacact     120

cgctgactga gaggctatac gtgggagggc ccatgctcaa tagcaaaggc cagacctgcg     180

ggtacaggcg ttgccgcgcc agcggggtgc tcaccactag catgggaaac accattacgt     240

gctatgtgaa agctctagcg gcatgcaagg ccgcagggat agtagcgccc acgatgctgg     300

tatgcggcga cgacctggtc gtcatctcag aaagccaggg gactgaggag gacgagcgga     360

acctgagagt cttcacggag gctatgacca ggaattccgc c                         401


<210> 32
<211> 401
<212> DNA
<213> Hepatitis C virus

<220> 
<221> source
<222> 1..401
<223> /mol_type="DNA"
      /note="Amplicon VHC long 4a/4d 401nt #PTR4162"
      /organism="Hepatitis C virus"

<400> 32
tggggatccc gtatgatacc cgctgctttg actccactgt aaccgaaaga gacatcaggg      60

tcgaggagga ggtctatcag tgttgtgacc tagagcccga agcccgcaag gtaatatccg     120

ccctcacaga gagactctac gtgggcggtc ccatgtacaa cagcagggga gacctttgcg     180

gaactcgacg gtgccgtgca agcggcgtat tcaccaccag ctttgggaac acactgacgt     240

gctatcttaa ggccagcgcg gccatcaggg ctgcaggcct aaaggactgc accatgctgg     300

tctgtggcga cgacttagtc gttatcgctg aaagcgatgg cgtggaggag gacaaccgtg     360

cgctcagagc cttcacggag gctatgacca ggaattccgc c                         401


<210> 33
<211> 108
<212> DNA
<213> Hepatitis C virus

<220> 
<221> source
<222> 1..108
<223> /mol_type="DNA"
      /note="Amplicon VHC court 108nt 2 #PTR7761"
      /organism="Hepatitis C virus"

<400> 33
atgctggtat gcggcgacga cctggtcgtc atctcagaaa gccaggggac tgaggaggac     60

gagcggaacc tgagagtctt cacggaggct atgaccagga attccgcc                 108


<210> 34
<211> 175
<212> DNA
<213> Hepatitis C virus

<220> 
<221> source
<222> 1..175
<223> /mol_type="DNA"
      /note="Amplicon VHC court 4a/4d 175nt #PTR4162"
      /organism="Hepatitis C virus"

<400> 34
tggggatccc gtatgatacc cgctgctttg actccactgt aaccgaaaga gacatcaggg     60

tcgaggagga ggtctatcag tgttgtgacc tagagcccga agcccgcaag gtaatatccg    120

ccctcacaga gagactctac gtgggcggtc ccatgtacaa cagcagggga gacct         175


<210> 35
<211> 15
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..15
<223> /mol_type="DNA"
      /note="Sonde VHC 2a/2c"
      /organism="artificial sequences"

<400> 35
ggactcctcr gttct                                                     15


<210> 36
<211> 15
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..15
<223> /mol_type="DNA"
      /note="Sonde VHC 2b"
      /organism="artificial sequences"

<400> 36
tatggattct tctgt                                                     15


<210> 37
<211> 15
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..15
<223> /mol_type="DNA"
      /note="Cible VHC 2a/2c 15-mères biotinylée"
      /organism="artificial sequences"

<400> 37
agaacygagg agtcc                                                     15


<210> 38
<211> 15
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..15
<223> /mol_type="DNA"
      /note="Cible VHC 2b 15-mères biotinylée"
      /organism="artificial sequences"

<400> 38
acagaagaat ccata                                                     15


<210> 39
<211> 15
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..15
<223> /mol_type="DNA"
      /note="Cible VHC 4a/4d 15-mères biotinylée"
      /organism="artificial sequences"

<400> 39
ctaygtgggc ggycc                                                     15


<210> 40
<211> 14
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..14
<223> /mol_type="DNA"
      /note="Sonde générique 3 flavivirus"
      /organism="artificial sequences"

<400> 40
agccacatgw acca                                                      14


<210> 41
<211> 15
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..15
<223> /mol_type="DNA"
      /note="Sonde spécifique Dengue 4"
      /organism="artificial sequences"

<400> 41
cactccactc catga                                                     15


<210> 42
<211> 15
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..15
<223> /mol_type="DNA"
      /note="Cible WNV 15-mères biotinylée"
      /organism="artificial sequences"

<400> 42
agaaytcagg aggmg                                                     15


<210> 43
<211> 13
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..13
<223> /mol_type="DNA"
      /note="Cible générique 3 flavivirus 15-mères biotinylée"
      /organism="artificial sequences"

<400> 43
tggwcatgtg gct                                                       13


<210> 44
<211> 15
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..15
<223> /mol_type="DNA"
      /note="Cible spécifique Dengue 4 15-mères biotinylée"
      /organism="artificial sequences"

<400> 44
tcatggagtg gagtg                                                     15


