                         SEQUENCE LISTING

<110>  GENENTECH, INC.
       HONGO, Jo-Anne
       MAO, Weiguang
       POLAKIS, Paul
       POLSON, Andrew
       VIJ, Rajesh
       WU, Yan
       LIANG, Wei-Ching
 
<120>  ANTI-LGR5 ANTIBODIES AND IMMUNOCONJUGATES

<130>  GENE-32548/WO-1/ORD

<150>  US 61/618,232
<151>  2012-03-30

<150>  US 61/683,048
<151>  2012-08-14

<150>  US 61/778,710
<151>  2013-03-13

<160>  86    

<170>  PatentIn version 3.5

<210>  1
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hukIV

<400>  1

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser 
            20                  25                  30          


Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Ser Thr Pro Phe Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys Arg 
        


<210>  2
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: huVH1

<400>  2

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Tyr Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Trp Ile Asn Pro Gly Ser Gly Asn Thr Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Arg Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
            100                 105                 110         


<210>  3
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu8E11 light chain variable region

<400>  3

Asn Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser Val Asp Asn Tyr 
            20                  25                  30          


Gly Asn Ser Phe Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Leu Ala Ser Asn Leu Glu Ser Gly Val Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Arg Thr Asp Phe Thr Leu Thr Ile Asp 
65                  70                  75                  80  


Pro Val Glu Ala Asp Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Asn Tyr 
                85                  90                  95      


Glu Asp Pro Phe Thr Phe Gly Ser Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110         


<210>  4
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu8E11 heavy chain variable region

<400>  4

Gln Val Gln Leu Gln Gln Ser Gly Thr Glu Leu Met Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Thr Gly Tyr Thr Phe Ser Ala Tyr 
            20                  25                  30          


Trp Ile Glu Trp Ile Lys Gln Arg Pro Gly His Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Leu Pro Gly Ser Asp Ser Thr Asp Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Val Lys Ala Thr Phe Ser Ser Asp Thr Ser Ser Asn Thr Val Tyr 
65                  70                  75                  80  


Ile Gln Leu Asn Ser Leu Thr Tyr Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly His Tyr Gly Ser Leu Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Thr Leu Lys Val Ser Ser 
        115             


<210>  5
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v1 light chain variable region

<400>  5

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ala Ser Glu Ser Val Asp Asn Tyr 
            20                  25                  30          


Gly Asn Ser Phe Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Leu Ala Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Asn Tyr 
                85                  90                  95      


Glu Asp Pro Phe Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110         


<210>  6
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v1 heavy chain variable region

<400>  6

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ser Ala Tyr 
            20                  25                  30          


Trp Ile Glu Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Leu Pro Gly Ser Asp Ser Thr Asp Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Val Arg Val Thr Ile Thr Ser Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Leu Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly His Tyr Gly Ser Leu Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  7
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v2 light chain variable region

<400>  7

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ala Ser Glu Ser Val Asp Asn Tyr 
            20                  25                  30          


Gly Asn Ser Phe Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Leu Ala Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Asn Tyr 
                85                  90                  95      


Glu Asp Pro Phe Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110         


<210>  8
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v2 heavy chain variable region

<400>  8

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ser Ala Tyr 
            20                  25                  30          


Trp Ile Glu Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Leu Pro Gly Ser Asp Ser Thr Asp Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Val Arg Ala Thr Phe Thr Ser Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Leu Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly His Tyr Gly Ser Leu Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  9
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v3 light chain variable region

<400>  9

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ala Ser Glu Ser Val Asp Asn Tyr 
            20                  25                  30          


Gly Asn Ser Phe Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Leu Ala Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Arg Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Asn Tyr 
                85                  90                  95      


Glu Asp Pro Phe Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110         


<210>  10
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v3 heavy chain variable region

<400>  10

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ser Ala Tyr 
            20                  25                  30          


Trp Ile Glu Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Leu Pro Gly Ser Asp Ser Thr Asp Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Val Arg Val Thr Ile Thr Ser Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Leu Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly His Tyr Gly Ser Leu Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  11
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v4 light chain variable region

<400>  11

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ala Ser Glu Ser Val Asp Asn Tyr 
            20                  25                  30          


Gly Asn Ser Phe Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Leu Ala Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Arg Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Asn Tyr 
                85                  90                  95      


Glu Asp Pro Phe Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110         


<210>  12
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v4 heavy chain variable region

<400>  12

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ser Ala Tyr 
            20                  25                  30          


Trp Ile Glu Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Leu Pro Gly Ser Asp Ser Thr Asp Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Val Arg Ala Thr Phe Thr Ser Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Leu Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly His Tyr Gly Ser Leu Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  13
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v5 light chain variable region

<400>  13

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ala Ser Glu Ser Val Asp Asn Tyr 
            20                  25                  30          


Gly Asn Ser Phe Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Leu Ala Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Asn Tyr 
                85                  90                  95      


Glu Asp Pro Phe Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110         


<210>  14
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v5 heavy chain variable region

<400>  14

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ser Ala Tyr 
            20                  25                  30          


Trp Ile Glu Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Leu Pro Gly Ser Asp Ser Thr Asp Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Val Arg Val Thr Ile Thr Arg Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly His Tyr Gly Ser Leu Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  15
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v6 light chain variable region

<400>  15

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ala Ser Glu Ser Val Asp Asn Tyr 
            20                  25                  30          


Gly Asn Ser Phe Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Leu Ala Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Asn Tyr 
                85                  90                  95      


Glu Asp Pro Phe Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110         


<210>  16
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v6 heavy chain variable region

<400>  16

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ser Ala Tyr 
            20                  25                  30          


Trp Ile Glu Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Leu Pro Gly Ser Asp Ser Thr Asp Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Val Arg Val Thr Ile Thr Ala Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly His Tyr Gly Ser Leu Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  17
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v7 light chain variable region

<400>  17

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ala Ser Glu Ser Val Asp Asn Tyr 
            20                  25                  30          


Gly Asn Ser Phe Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Leu Ala Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Arg Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Asn Tyr 
                85                  90                  95      


Glu Asp Pro Phe Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110         


<210>  18
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v7 heavy chain variable region

<400>  18

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ser Ala Tyr 
            20                  25                  30          


Trp Ile Glu Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Leu Pro Gly Ser Asp Ser Thr Asp Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Val Arg Val Thr Ile Thr Arg Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly His Tyr Gly Ser Leu Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  19
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v8 light chain variable region

<400>  19

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ala Ser Glu Ser Val Asp Asn Tyr 
            20                  25                  30          


Gly Asn Ser Phe Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Leu Ala Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Arg Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Asn Tyr 
                85                  90                  95      


Glu Asp Pro Phe Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110         


<210>  20
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v8 heavy chain variable region

<400>  20

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ser Ala Tyr 
            20                  25                  30          


Trp Ile Glu Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Leu Pro Gly Ser Asp Ser Thr Asp Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Val Arg Val Thr Ile Thr Ala Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly His Tyr Gly Ser Leu Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  21
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu3G12 light chain variable region

<400>  21

Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu Gln Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Ile Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Thr His Phe Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg 
    


<210>  22
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu3G12 heavy chain variable region

<400>  22

Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Met Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Val Asp Thr Phe Asn Ser Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Asn Pro Ser Asn Gly Arg Thr Asn Tyr Ile Glu Lys Phe 
    50                  55                  60                  


Lys Asn Arg Ala Thr Val Thr Val Asp Lys Ser Ser Ser Thr Ala Phe 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Gly Trp Tyr Phe Asp Val Trp Gly Ala Gly Thr Thr Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  23
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu2H6 light chain variable region

<400>  23

Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Thr Val Thr Ala Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Thr Trp Phe Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Asn Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp Tyr Ser Phe Pro Phe Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys Arg 
        


<210>  24
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu2H6 heavy chain variable region

<400>  24

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Thr 
1               5                   10                  15      


Ser Met Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr 
            20                  25                  30          


Thr Met Asn Trp Val Lys Gln Ser His Lys Asn Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Leu Ile Asn Cys Tyr Asn Gly Gly Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Phe 
65                  70                  75                  80  


Met Glu Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Ser Thr Met Ile Thr Pro Arg Phe Ala Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120     


<210>  25
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YW353 light chain variable region

<400>  25

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105             


<210>  26
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YW353 heavy chain variable region

<400>  26

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Ser Tyr 
            20                  25                  30          


Ser Ile Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Glu Ile Tyr Pro Pro Gly Gly Tyr Thr Asp Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Ala Arg Leu Phe Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  27
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu8E11 HVR L1

<400>  27

Arg Ala Ser Glu Ser Val Asp Asn Tyr Gly Asn Ser Phe Met His 
1               5                   10                  15  


<210>  28
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu8E11 HVR L2

<400>  28

Leu Ala Ser Asn Leu Glu Ser 
1               5           


<210>  29
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu8E11 HVR L3

<400>  29

Gln Gln Asn Tyr Glu Asp Pro Phe Thr 
1               5                   


<210>  30
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu8E11 HVR H1

<400>  30

Gly Tyr Thr Phe Ser Ala Tyr Trp Ile Glu 
1               5                   10  


<210>  31
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu8E11 HVR H2

<400>  31

Glu Ile Leu Pro Gly Ser Asp Ser Thr Asp Tyr Asn Glu Lys Phe Lys 
1               5                   10                  15      


Val 
    


<210>  32
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu8E11 HVR H3

<400>  32

Gly Gly His Tyr Gly Ser Leu Asp Tyr 
1               5                   


<210>  33
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Hu8E11 light chain (LC) framework 1 (FR1)

<400>  33

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys 
            20              


<210>  34
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Hu8E11 LC FR2

<400>  34

Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr 
1               5                   10                  15  


<210>  35
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Hu8E11.v1 LC FR3/Hu8E11.v2 LC FR3/Hu8E11.v5 LC 
       FR3/Hu8E11.v6 LC FR3

<400>  35

Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
1               5                   10                  15      


Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys 
            20                  25                  30          


<210>  36
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Hu8E11.v3 LC FR3/Hu8E11.v4 LC FR3/Hu8E11.v7 LC 
       FR3/Hu8E11.v8 LC FR3

<400>  36

Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Arg Thr Asp Phe Thr 
1               5                   10                  15      


Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys 
            20                  25                  30          


<210>  37
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Hu8E11 LC FR4

<400>  37

Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
1               5                   10      


<210>  38
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Hu8E11 heavy chain (HC) framework1 (FR1)

<400>  38

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser 
            20                  25  


<210>  39
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Hu8E11 HC FR2

<400>  39

Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile Gly 
1               5                   10                  


<210>  40
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Hu8E11.v1 HC FR3/Hu8E11.v3 HC FR3

<400>  40

Arg Val Thr Ile Thr Ser Asp Thr Ser Thr Ser Thr Val Tyr Leu Glu 
1               5                   10                  15      


Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg 
            20                  25                  30          


<210>  41
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Hu8E11.v2 HC FR3/Hu8E11.v4 HC FR3

<400>  41

Arg Ala Thr Phe Thr Ser Asp Thr Ser Thr Ser Thr Val Tyr Leu Glu 
1               5                   10                  15      


Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg 
            20                  25                  30          


<210>  42
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Hu8E11.v5 HC FR3/Hu8E11.v7 HC FR3

<400>  42

Arg Val Thr Ile Thr Arg Asp Thr Ser Thr Ser Thr Ala Tyr Leu Glu 
1               5                   10                  15      


Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg 
            20                  25                  30          


<210>  43
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Hu8E11.v6 HC FR3/Hu8E11.v8 HC FR3

<400>  43

Arg Val Thr Ile Thr Ala Asp Thr Ser Thr Ser Thr Ala Tyr Leu Glu 
1               5                   10                  15      


Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg 
            20                  25                  30          


<210>  44
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Hu8E11 HC FR4

<400>  44

Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
1               5                   10      


<210>  45
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu3G12 HVR L1

<400>  45

Arg Ser Ser Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu Gln 
1               5                   10                  15      


<210>  46
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu3G12 HVR L2

<400>  46

Lys Val Ser Asn Arg Phe Ser 
1               5           


<210>  47
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu3G12 HVR L3

<400>  47

Ser Gln Ser Thr His Phe Pro Tyr Thr 
1               5                   


<210>  48
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu3G12 HVR H1

<400>  48

Val Asp Thr Phe Asn Ser Tyr Trp Met His 
1               5                   10  


<210>  49
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu3G12 HVR H2

<400>  49

Glu Ile Asn Pro Ser Asn Gly Arg Thr Asn Tyr Ile Glu Lys Phe Lys 
1               5                   10                  15      


Asn 
    


<210>  50
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu3G12 HVR H3

<400>  50

Gly Trp Tyr Phe Asp Val 
1               5       


<210>  51
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu2H6 HVR L1

<400>  51

Lys Ser Ser Gln Ser Leu Leu Asn Ser Gly Asn Gln Lys Asn Tyr Leu 
1               5                   10                  15      


Thr 
    


<210>  52
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu2H6 HVR L2

<400>  52

Trp Ala Ser Thr Arg Glu Ser 
1               5           


<210>  53
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu2H6 HVR L3

<400>  53

Gln Asn Asp Tyr Ser Phe Pro Phe Thr 
1               5                   


<210>  54
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu2H6 HVR H1

<400>  54

Gly Tyr Ser Phe Thr Gly Tyr Thr Met Asn 
1               5                   10  


<210>  55
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu2H6 HVR H2

<400>  55

Leu Ile Asn Cys Tyr Asn Gly Gly Thr Asn Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  56
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: mu2H6 HVR H3

<400>  56

Gly Gly Ser Thr Met Ile Thr Pro Arg Phe Ala Tyr 
1               5                   10          


<210>  57
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YW353 HVR L1

<400>  57

Arg Ala Ser Gln Asp Val Ser Thr Ala Val Ala 
1               5                   10      


<210>  58
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YW353 HVR L2

<400>  58

Ser Ala Ser Phe Leu Tyr Ser 
1               5           


<210>  59
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YW353 HVR L3

<400>  59

Gln Gln Ser Tyr Thr Thr Pro Pro Thr 
1               5                   


<210>  60
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YW353 HVR H1

<400>  60

Gly Phe Thr Phe Thr Ser Tyr Ser Ile Ser 
1               5                   10  


<210>  61
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YW353 HVR H2

<400>  61

Glu Ile Tyr Pro Pro Gly Gly Tyr Thr Asp Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  62
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YW353 HVR H3

<400>  62

Ala Arg Leu Phe Phe Asp Tyr 
1               5           


<210>  63
<211>  218
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v2 light chain

<400>  63

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ala Ser Glu Ser Val Asp Asn Tyr 
            20                  25                  30          


Gly Asn Ser Phe Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Leu Ala Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Asn Tyr 
                85                  90                  95      


Glu Asp Pro Phe Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110         


Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
        115                 120                 125             


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
    130                 135                 140                 


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
145                 150                 155                 160 


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
                165                 170                 175     


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
            180                 185                 190         


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
        195                 200                 205             


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215             


<210>  64
<211>  448
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v2 heavy chain

<400>  64

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ser Ala Tyr 
            20                  25                  30          


Trp Ile Glu Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Leu Pro Gly Ser Asp Ser Thr Asp Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Val Arg Ala Thr Phe Thr Ser Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Leu Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly His Tyr Gly Ser Leu Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr 
    210                 215                 220                 


His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 
225                 230                 235                 240 


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
                245                 250                 255     


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
            260                 265                 270         


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
        275                 280                 285             


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
    290                 295                 300                 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
305                 310                 315                 320 


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 
                325                 330                 335     


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
            340                 345                 350         


Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
        355                 360                 365             


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
    370                 375                 380                 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
385                 390                 395                 400 


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
                405                 410                 415     


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
            420                 425                 430         


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445             


<210>  65
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YW353 light chain

<400>  65

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  66
<211>  446
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YW353 heavy chain

<400>  66

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Ser Tyr 
            20                  25                  30          


Ser Ile Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Glu Ile Tyr Pro Pro Gly Gly Tyr Thr Asp Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Ala Arg Leu Phe Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala 
        115                 120                 125             


Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu 
    130                 135                 140                 


Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly 
145                 150                 155                 160 


Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser 
                165                 170                 175     


Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu 
            180                 185                 190         


Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr 
        195                 200                 205             


Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr 
    210                 215                 220                 


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
225                 230                 235                 240 


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
                245                 250                 255     


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
            260                 265                 270         


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
        275                 280                 285             


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
    290                 295                 300                 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
305                 310                 315                 320 


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
                325                 330                 335     


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
            340                 345                 350         


Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
        355                 360                 365             


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
    370                 375                 380                 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
385                 390                 395                 400 


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
                405                 410                 415     


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
            420                 425                 430         


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445     


<210>  67
<211>  907
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Human LgR5 precursor; LGR5_human NP_003658; signal sequence = 
       amino acids 1-21

<400>  67

Met Asp Thr Ser Arg Leu Gly Val Leu Leu Ser Leu Pro Val Leu Leu 
1               5                   10                  15      


Gln Leu Ala Thr Gly Gly Ser Ser Pro Arg Ser Gly Val Leu Leu Arg 
            20                  25                  30          


Gly Cys Pro Thr His Cys His Cys Glu Pro Asp Gly Arg Met Leu Leu 
        35                  40                  45              


Arg Val Asp Cys Ser Asp Leu Gly Leu Ser Glu Leu Pro Ser Asn Leu 
    50                  55                  60                  


Ser Val Phe Thr Ser Tyr Leu Asp Leu Ser Met Asn Asn Ile Ser Gln 
65                  70                  75                  80  


Leu Leu Pro Asn Pro Leu Pro Ser Leu Arg Phe Leu Glu Glu Leu Arg 
                85                  90                  95      


Leu Ala Gly Asn Ala Leu Thr Tyr Ile Pro Lys Gly Ala Phe Thr Gly 
            100                 105                 110         


Leu Tyr Ser Leu Lys Val Leu Met Leu Gln Asn Asn Gln Leu Arg His 
        115                 120                 125             


Val Pro Thr Glu Ala Leu Gln Asn Leu Arg Ser Leu Gln Ser Leu Arg 
    130                 135                 140                 


Leu Asp Ala Asn His Ile Ser Tyr Val Pro Pro Ser Cys Phe Ser Gly 
145                 150                 155                 160 


Leu His Ser Leu Arg His Leu Trp Leu Asp Asp Asn Ala Leu Thr Glu 
                165                 170                 175     


Ile Pro Val Gln Ala Phe Arg Ser Leu Ser Ala Leu Gln Ala Met Thr 
            180                 185                 190         


Leu Ala Leu Asn Lys Ile His His Ile Pro Asp Tyr Ala Phe Gly Asn 
        195                 200                 205             


Leu Ser Ser Leu Val Val Leu His Leu His Asn Asn Arg Ile His Ser 
    210                 215                 220                 


Leu Gly Lys Lys Cys Phe Asp Gly Leu His Ser Leu Glu Thr Leu Asp 
225                 230                 235                 240 


Leu Asn Tyr Asn Asn Leu Asp Glu Phe Pro Thr Ala Ile Arg Thr Leu 
                245                 250                 255     


Ser Asn Leu Lys Glu Leu Gly Phe His Ser Asn Asn Ile Arg Ser Ile 
            260                 265                 270         


Pro Glu Lys Ala Phe Val Gly Asn Pro Ser Leu Ile Thr Ile His Phe 
        275                 280                 285             


Tyr Asp Asn Pro Ile Gln Phe Val Gly Arg Ser Ala Phe Gln His Leu 
    290                 295                 300                 


Pro Glu Leu Arg Thr Leu Thr Leu Asn Gly Ala Ser Gln Ile Thr Glu 
305                 310                 315                 320 


Phe Pro Asp Leu Thr Gly Thr Ala Asn Leu Glu Ser Leu Thr Leu Thr 
                325                 330                 335     


Gly Ala Gln Ile Ser Ser Leu Pro Gln Thr Val Cys Asn Gln Leu Pro 
            340                 345                 350         


Asn Leu Gln Val Leu Asp Leu Ser Tyr Asn Leu Leu Glu Asp Leu Pro 
        355                 360                 365             


Ser Phe Ser Val Cys Gln Lys Leu Gln Lys Ile Asp Leu Arg His Asn 
    370                 375                 380                 


Glu Ile Tyr Glu Ile Lys Val Asp Thr Phe Gln Gln Leu Leu Ser Leu 
385                 390                 395                 400 


Arg Ser Leu Asn Leu Ala Trp Asn Lys Ile Ala Ile Ile His Pro Asn 
                405                 410                 415     


Ala Phe Ser Thr Leu Pro Ser Leu Ile Lys Leu Asp Leu Ser Ser Asn 
            420                 425                 430         


Leu Leu Ser Ser Phe Pro Ile Thr Gly Leu His Gly Leu Thr His Leu 
        435                 440                 445             


Lys Leu Thr Gly Asn His Ala Leu Gln Ser Leu Ile Ser Ser Glu Asn 
    450                 455                 460                 


Phe Pro Glu Leu Lys Val Ile Glu Met Pro Tyr Ala Tyr Gln Cys Cys 
465                 470                 475                 480 


Ala Phe Gly Val Cys Glu Asn Ala Tyr Lys Ile Ser Asn Gln Trp Asn 
                485                 490                 495     


Lys Gly Asp Asn Ser Ser Met Asp Asp Leu His Lys Lys Asp Ala Gly 
            500                 505                 510         


Met Phe Gln Ala Gln Asp Glu Arg Asp Leu Glu Asp Phe Leu Leu Asp 
        515                 520                 525             


Phe Glu Glu Asp Leu Lys Ala Leu His Ser Val Gln Cys Ser Pro Ser 
    530                 535                 540                 


Pro Gly Pro Phe Lys Pro Cys Glu His Leu Leu Asp Gly Trp Leu Ile 
545                 550                 555                 560 


Arg Ile Gly Val Trp Thr Ile Ala Val Leu Ala Leu Thr Cys Asn Ala 
                565                 570                 575     


Leu Val Thr Ser Thr Val Phe Arg Ser Pro Leu Tyr Ile Ser Pro Ile 
            580                 585                 590         


Lys Leu Leu Ile Gly Val Ile Ala Ala Val Asn Met Leu Thr Gly Val 
        595                 600                 605             


Ser Ser Ala Val Leu Ala Gly Val Asp Ala Phe Thr Phe Gly Ser Phe 
    610                 615                 620                 


Ala Arg His Gly Ala Trp Trp Glu Asn Gly Val Gly Cys His Val Ile 
625                 630                 635                 640 


Gly Phe Leu Ser Ile Phe Ala Ser Glu Ser Ser Val Phe Leu Leu Thr 
                645                 650                 655     


Leu Ala Ala Leu Glu Arg Gly Phe Ser Val Lys Tyr Ser Ala Lys Phe 
            660                 665                 670         


Glu Thr Lys Ala Pro Phe Ser Ser Leu Lys Val Ile Ile Leu Leu Cys 
        675                 680                 685             


Ala Leu Leu Ala Leu Thr Met Ala Ala Val Pro Leu Leu Gly Gly Ser 
    690                 695                 700                 


Lys Tyr Gly Ala Ser Pro Leu Cys Leu Pro Leu Pro Phe Gly Glu Pro 
705                 710                 715                 720 


Ser Thr Met Gly Tyr Met Val Ala Leu Ile Leu Leu Asn Ser Leu Cys 
                725                 730                 735     


Phe Leu Met Met Thr Ile Ala Tyr Thr Lys Leu Tyr Cys Asn Leu Asp 
            740                 745                 750         


Lys Gly Asp Leu Glu Asn Ile Trp Asp Cys Ser Met Val Lys His Ile 
        755                 760                 765             


Ala Leu Leu Leu Phe Thr Asn Cys Ile Leu Asn Cys Pro Val Ala Phe 
    770                 775                 780                 


Leu Ser Phe Ser Ser Leu Ile Asn Leu Thr Phe Ile Ser Pro Glu Val 
785                 790                 795                 800 


Ile Lys Phe Ile Leu Leu Val Val Val Pro Leu Pro Ala Cys Leu Asn 
                805                 810                 815     


Pro Leu Leu Tyr Ile Leu Phe Asn Pro His Phe Lys Glu Asp Leu Val 
            820                 825                 830         


Ser Leu Arg Lys Gln Thr Tyr Val Trp Thr Arg Ser Lys His Pro Ser 
        835                 840                 845             


Leu Met Ser Ile Asn Ser Asp Asp Val Glu Lys Gln Ser Cys Asp Ser 
    850                 855                 860                 


Thr Gln Ala Leu Val Thr Phe Thr Ser Ser Ser Ile Thr Tyr Asp Leu 
865                 870                 875                 880 


Pro Pro Ser Ser Val Pro Ser Pro Ala Tyr Pro Val Thr Glu Ser Cys 
                885                 890                 895     


His Leu Ser Ser Val Ala Phe Val Pro Cys Leu 
            900                 905         


<210>  68
<211>  886
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Human LgR5 mature, without signal sequence; amino acids 22 to 907

<400>  68

Gly Ser Ser Pro Arg Ser Gly Val Leu Leu Arg Gly Cys Pro Thr His 
1               5                   10                  15      


Cys His Cys Glu Pro Asp Gly Arg Met Leu Leu Arg Val Asp Cys Ser 
            20                  25                  30          


Asp Leu Gly Leu Ser Glu Leu Pro Ser Asn Leu Ser Val Phe Thr Ser 
        35                  40                  45              


Tyr Leu Asp Leu Ser Met Asn Asn Ile Ser Gln Leu Leu Pro Asn Pro 
    50                  55                  60                  


Leu Pro Ser Leu Arg Phe Leu Glu Glu Leu Arg Leu Ala Gly Asn Ala 
65                  70                  75                  80  


Leu Thr Tyr Ile Pro Lys Gly Ala Phe Thr Gly Leu Tyr Ser Leu Lys 
                85                  90                  95      


Val Leu Met Leu Gln Asn Asn Gln Leu Arg His Val Pro Thr Glu Ala 
            100                 105                 110         


Leu Gln Asn Leu Arg Ser Leu Gln Ser Leu Arg Leu Asp Ala Asn His 
        115                 120                 125             


Ile Ser Tyr Val Pro Pro Ser Cys Phe Ser Gly Leu His Ser Leu Arg 
    130                 135                 140                 


His Leu Trp Leu Asp Asp Asn Ala Leu Thr Glu Ile Pro Val Gln Ala 
145                 150                 155                 160 


Phe Arg Ser Leu Ser Ala Leu Gln Ala Met Thr Leu Ala Leu Asn Lys 
                165                 170                 175     


Ile His His Ile Pro Asp Tyr Ala Phe Gly Asn Leu Ser Ser Leu Val 
            180                 185                 190         


Val Leu His Leu His Asn Asn Arg Ile His Ser Leu Gly Lys Lys Cys 
        195                 200                 205             


Phe Asp Gly Leu His Ser Leu Glu Thr Leu Asp Leu Asn Tyr Asn Asn 
    210                 215                 220                 


Leu Asp Glu Phe Pro Thr Ala Ile Arg Thr Leu Ser Asn Leu Lys Glu 
225                 230                 235                 240 


Leu Gly Phe His Ser Asn Asn Ile Arg Ser Ile Pro Glu Lys Ala Phe 
                245                 250                 255     


Val Gly Asn Pro Ser Leu Ile Thr Ile His Phe Tyr Asp Asn Pro Ile 
            260                 265                 270         


Gln Phe Val Gly Arg Ser Ala Phe Gln His Leu Pro Glu Leu Arg Thr 
        275                 280                 285             


Leu Thr Leu Asn Gly Ala Ser Gln Ile Thr Glu Phe Pro Asp Leu Thr 
    290                 295                 300                 


Gly Thr Ala Asn Leu Glu Ser Leu Thr Leu Thr Gly Ala Gln Ile Ser 
305                 310                 315                 320 


Ser Leu Pro Gln Thr Val Cys Asn Gln Leu Pro Asn Leu Gln Val Leu 
                325                 330                 335     


Asp Leu Ser Tyr Asn Leu Leu Glu Asp Leu Pro Ser Phe Ser Val Cys 
            340                 345                 350         


Gln Lys Leu Gln Lys Ile Asp Leu Arg His Asn Glu Ile Tyr Glu Ile 
        355                 360                 365             


Lys Val Asp Thr Phe Gln Gln Leu Leu Ser Leu Arg Ser Leu Asn Leu 
    370                 375                 380                 


Ala Trp Asn Lys Ile Ala Ile Ile His Pro Asn Ala Phe Ser Thr Leu 
385                 390                 395                 400 


Pro Ser Leu Ile Lys Leu Asp Leu Ser Ser Asn Leu Leu Ser Ser Phe 
                405                 410                 415     


Pro Ile Thr Gly Leu His Gly Leu Thr His Leu Lys Leu Thr Gly Asn 
            420                 425                 430         


His Ala Leu Gln Ser Leu Ile Ser Ser Glu Asn Phe Pro Glu Leu Lys 
        435                 440                 445             


Val Ile Glu Met Pro Tyr Ala Tyr Gln Cys Cys Ala Phe Gly Val Cys 
    450                 455                 460                 


Glu Asn Ala Tyr Lys Ile Ser Asn Gln Trp Asn Lys Gly Asp Asn Ser 
465                 470                 475                 480 


Ser Met Asp Asp Leu His Lys Lys Asp Ala Gly Met Phe Gln Ala Gln 
                485                 490                 495     


Asp Glu Arg Asp Leu Glu Asp Phe Leu Leu Asp Phe Glu Glu Asp Leu 
            500                 505                 510         


Lys Ala Leu His Ser Val Gln Cys Ser Pro Ser Pro Gly Pro Phe Lys 
        515                 520                 525             


Pro Cys Glu His Leu Leu Asp Gly Trp Leu Ile Arg Ile Gly Val Trp 
    530                 535                 540                 


Thr Ile Ala Val Leu Ala Leu Thr Cys Asn Ala Leu Val Thr Ser Thr 
545                 550                 555                 560 


Val Phe Arg Ser Pro Leu Tyr Ile Ser Pro Ile Lys Leu Leu Ile Gly 
                565                 570                 575     


Val Ile Ala Ala Val Asn Met Leu Thr Gly Val Ser Ser Ala Val Leu 
            580                 585                 590         


Ala Gly Val Asp Ala Phe Thr Phe Gly Ser Phe Ala Arg His Gly Ala 
        595                 600                 605             


Trp Trp Glu Asn Gly Val Gly Cys His Val Ile Gly Phe Leu Ser Ile 
    610                 615                 620                 


Phe Ala Ser Glu Ser Ser Val Phe Leu Leu Thr Leu Ala Ala Leu Glu 
625                 630                 635                 640 


Arg Gly Phe Ser Val Lys Tyr Ser Ala Lys Phe Glu Thr Lys Ala Pro 
                645                 650                 655     


Phe Ser Ser Leu Lys Val Ile Ile Leu Leu Cys Ala Leu Leu Ala Leu 
            660                 665                 670         


Thr Met Ala Ala Val Pro Leu Leu Gly Gly Ser Lys Tyr Gly Ala Ser 
        675                 680                 685             


Pro Leu Cys Leu Pro Leu Pro Phe Gly Glu Pro Ser Thr Met Gly Tyr 
    690                 695                 700                 


Met Val Ala Leu Ile Leu Leu Asn Ser Leu Cys Phe Leu Met Met Thr 
705                 710                 715                 720 


Ile Ala Tyr Thr Lys Leu Tyr Cys Asn Leu Asp Lys Gly Asp Leu Glu 
                725                 730                 735     


Asn Ile Trp Asp Cys Ser Met Val Lys His Ile Ala Leu Leu Leu Phe 
            740                 745                 750         


Thr Asn Cys Ile Leu Asn Cys Pro Val Ala Phe Leu Ser Phe Ser Ser 
        755                 760                 765             


Leu Ile Asn Leu Thr Phe Ile Ser Pro Glu Val Ile Lys Phe Ile Leu 
    770                 775                 780                 


Leu Val Val Val Pro Leu Pro Ala Cys Leu Asn Pro Leu Leu Tyr Ile 
785                 790                 795                 800 


Leu Phe Asn Pro His Phe Lys Glu Asp Leu Val Ser Leu Arg Lys Gln 
                805                 810                 815     


Thr Tyr Val Trp Thr Arg Ser Lys His Pro Ser Leu Met Ser Ile Asn 
            820                 825                 830         


Ser Asp Asp Val Glu Lys Gln Ser Cys Asp Ser Thr Gln Ala Leu Val 
        835                 840                 845             


Thr Phe Thr Ser Ser Ser Ile Thr Tyr Asp Leu Pro Pro Ser Ser Val 
    850                 855                 860                 


Pro Ser Pro Ala Tyr Pro Val Thr Glu Ser Cys His Leu Ser Ser Val 
865                 870                 875                 880 


Ala Phe Val Pro Cys Leu 
                885     


<210>  69
<211>  875
<212>  PRT
<213>  M. cynomolgus


<220>
<221>  misc_feature
<223>  Cynomolgus monkey LgR5 partial sequence, predicted; predicted to 
       correspond to amino acids 33 to 907 of full-length precursor

<400>  69

Gly Cys Pro Thr His Cys His Cys Glu Pro Asp Gly Arg Met Leu Leu 
1               5                   10                  15      


Arg Val Asp Cys Ser Asp Leu Gly Leu Ser Glu Leu Pro Ser Asn Leu 
            20                  25                  30          


Ser Val Phe Thr Ser Tyr Leu Asp Leu Ser Met Asn Asn Ile Ser Gln 
        35                  40                  45              


Leu Leu Pro Asn Pro Leu Pro Ser Leu Arg Phe Leu Glu Glu Leu Arg 
    50                  55                  60                  


Leu Ala Gly Asn Ala Leu Thr Tyr Ile Pro Lys Gly Ala Phe Thr Gly 
65                  70                  75                  80  


Leu Tyr Ser Leu Lys Val Leu Met Leu Gln Asn Asn Gln Leu Arg Gln 
                85                  90                  95      


Val Pro Thr Glu Ala Leu Gln Asn Leu Arg Ser Leu Gln Ser Leu Arg 
            100                 105                 110         


Leu Asp Ala Asn His Ile Ser Tyr Val Pro Pro Ser Cys Phe Ser Gly 
        115                 120                 125             


Leu His Ser Leu Arg His Leu Trp Leu Asp Asp Asn Ala Leu Thr Glu 
    130                 135                 140                 


Ile Pro Val Gln Ala Phe Arg Ser Leu Ser Ala Leu Gln Ala Met Thr 
145                 150                 155                 160 


Leu Ala Leu Asn Lys Ile His His Ile Pro Asp Tyr Ala Phe Gly Asn 
                165                 170                 175     


Leu Ser Ser Leu Val Val Leu His Leu His Asn Asn Arg Ile His Ser 
            180                 185                 190         


Leu Gly Lys Lys Cys Phe Asp Gly Leu His Ser Leu Glu Thr Leu Asp 
        195                 200                 205             


Leu Asn Tyr Asn Asn Leu Asp Glu Phe Pro Thr Ala Ile Arg Thr Leu 
    210                 215                 220                 


Ser Asn Leu Lys Glu Leu Gly Phe His Ser Asn Asn Ile Arg Ser Ile 
225                 230                 235                 240 


Pro Glu Lys Ala Phe Val Gly Asn Pro Ser Leu Ile Thr Ile His Phe 
                245                 250                 255     


Tyr Asp Asn Pro Ile Gln Phe Val Gly Arg Ser Ala Phe Gln His Leu 
            260                 265                 270         


Pro Glu Leu Arg Thr Leu Thr Leu Asn Gly Ala Ser Gln Ile Thr Glu 
        275                 280                 285             


Phe Pro Asp Leu Thr Gly Thr Ala Asn Leu Glu Ser Leu Thr Leu Thr 
    290                 295                 300                 


Gly Ala Gln Ile Ser Ser Leu Pro Gln Thr Val Cys Asn Gln Leu Pro 
305                 310                 315                 320 


Asn Leu Gln Val Leu Asp Leu Ser Tyr Asn Leu Leu Glu Asp Leu Pro 
                325                 330                 335     


Ser Phe Ser Val Cys Gln Lys Leu Gln Lys Ile Asp Leu Arg His Asn 
            340                 345                 350         


Glu Ile Tyr Glu Ile Lys Val Asp Thr Phe Gln Gln Leu Leu Ser Leu 
        355                 360                 365             


Arg Ser Leu Asn Leu Ala Trp Asn Lys Ile Ala Ile Ile His Pro Asn 
    370                 375                 380                 


Ala Phe Ser Thr Leu Pro Ser Leu Ile Lys Leu Asp Leu Ser Ser Asn 
385                 390                 395                 400 


Leu Leu Ser Ser Phe Pro Val Thr Gly Leu His Gly Leu Thr His Leu 
                405                 410                 415     


Lys Leu Thr Gly Asn His Ala Leu Gln Ser Leu Ile Ser Ser Glu Asn 
            420                 425                 430         


Phe Pro Glu Leu Lys Ile Ile Glu Met Pro Tyr Ala Tyr Gln Cys Cys 
        435                 440                 445             


Ala Phe Gly Val Cys Glu Asn Ala Tyr Lys Ile Ser Asn Gln Trp Asn 
    450                 455                 460                 


Lys Gly Asp Asn Ser Ser Met Asp Asp Leu His Lys Lys Asp Ala Gly 
465                 470                 475                 480 


Met Phe Gln Val Gln Asp Glu Arg Asp Leu Glu Asp Phe Leu Leu Asp 
                485                 490                 495     


Phe Glu Glu Asp Leu Lys Ala Leu His Ser Val Gln Cys Ser Pro Ser 
            500                 505                 510         


Pro Gly Pro Phe Lys Pro Cys Glu His Leu Leu Asp Gly Trp Leu Ile 
        515                 520                 525             


Arg Ile Gly Val Trp Thr Ile Ala Val Leu Ala Leu Thr Cys Asn Ala 
    530                 535                 540                 


Leu Val Thr Ser Thr Val Phe Arg Ser Pro Leu Tyr Ile Ser Pro Ile 
545                 550                 555                 560 


Lys Leu Leu Ile Gly Val Ile Ala Val Val Asn Met Leu Thr Gly Val 
                565                 570                 575     


Ser Ser Ala Val Leu Ala Gly Val Asp Ala Phe Thr Phe Gly Ser Phe 
            580                 585                 590         


Ala Arg His Gly Ala Trp Trp Glu Asn Gly Val Gly Cys Gln Val Ile 
        595                 600                 605             


Gly Phe Leu Ser Ile Phe Ala Ser Glu Ser Ser Val Phe Leu Leu Thr 
    610                 615                 620                 


Leu Ala Ala Leu Glu Arg Gly Phe Ser Val Lys Cys Ser Ala Lys Phe 
625                 630                 635                 640 


Glu Thr Lys Ala Pro Phe Ser Ser Leu Lys Val Ile Ile Leu Leu Cys 
                645                 650                 655     


Ala Leu Leu Ala Leu Thr Met Ala Ala Val Pro Leu Leu Gly Gly Ser 
            660                 665                 670         


Glu Tyr Gly Ala Ser Pro Leu Cys Leu Pro Leu Pro Phe Gly Glu Pro 
        675                 680                 685             


Ser Thr Thr Gly Tyr Met Val Ala Leu Ile Leu Leu Asn Ser Leu Cys 
    690                 695                 700                 


Phe Leu Met Met Thr Ile Ala Tyr Thr Lys Leu Tyr Cys Asn Leu Asp 
705                 710                 715                 720 


Lys Gly Asp Leu Glu Asn Ile Trp Asp Cys Ser Met Val Lys His Ile 
                725                 730                 735     


Ala Leu Leu Leu Phe Thr Asn Cys Ile Leu Tyr Cys Pro Val Ala Phe 
            740                 745                 750         


Leu Ser Phe Ser Ser Leu Leu Asn Leu Thr Phe Ile Ser Pro Glu Val 
        755                 760                 765             


Ile Lys Phe Ile Leu Leu Val Ile Val Pro Leu Pro Ala Cys Leu Asn 
    770                 775                 780                 


Pro Leu Leu Tyr Ile Leu Phe Asn Pro His Phe Lys Glu Asp Leu Val 
785                 790                 795                 800 


Ser Leu Gly Lys Gln Thr Tyr Phe Trp Thr Arg Ser Lys His Pro Ser 
                805                 810                 815     


Leu Met Ser Ile Asn Ser Asp Asp Val Glu Lys Gln Ser Cys Asp Ser 
            820                 825                 830         


Thr Gln Ala Leu Val Thr Phe Thr Ser Ser Ser Ile Ala Tyr Asp Leu 
        835                 840                 845             


Pro Pro Ser Ser Val Pro Ser Pro Ala Tyr Pro Val Thr Glu Ser Cys 
    850                 855                 860                 


His Leu Ser Ser Val Ala Phe Val Pro Cys Leu 
865                 870                 875 


<210>  70
<211>  907
<212>  PRT
<213>  Rattus sp.


<220>
<221>  misc_feature
<223>  Rat LgR5 precursor; LGR5_rat NP_001100254; signal sequence = 
       amino acids 1-21

<400>  70

Met Asp Thr Ser Arg Val Arg Met Leu Leu Ser Leu Leu Ala Leu Leu 
1               5                   10                  15      


Gln Leu Val Ala Ala Gly Ser Pro Pro Arg Pro Asp Thr Met Pro Arg 
            20                  25                  30          


Gly Cys Pro Ser Tyr Cys His Cys Glu Leu Asp Gly Arg Met Leu Leu 
        35                  40                  45              


Arg Val Asp Cys Ser Asp Leu Gly Leu Ser Glu Leu Pro Ser Asn Leu 
    50                  55                  60                  


Ser Val Phe Thr Ser Tyr Leu Asp Leu Ser Met Asn Asn Ile Ser Gln 
65                  70                  75                  80  


Leu Pro Ala Ser Leu Leu His Arg Leu Arg Phe Leu Glu Glu Leu Arg 
                85                  90                  95      


Leu Ala Gly Asn Ala Leu Thr His Ile Pro Lys Gly Ala Phe Ala Gly 
            100                 105                 110         


Leu His Ser Leu Lys Val Leu Met Leu Gln Asn Asn Gln Leu Arg Gln 
        115                 120                 125             


Val Pro Glu Glu Ala Leu Gln Asn Leu Arg Ser Leu Gln Ser Leu Arg 
    130                 135                 140                 


Leu Asp Ala Asn His Ile Ser Tyr Val Pro Pro Ser Cys Phe Ser Gly 
145                 150                 155                 160 


Leu His Ser Leu Arg His Leu Trp Leu Asp Asp Asn Ala Leu Thr Asp 
                165                 170                 175     


Val Pro Val Gln Ala Phe Arg Ser Leu Ser Ala Leu Gln Ala Met Thr 
            180                 185                 190         


Leu Ala Leu Asn Lys Ile His His Ile Ala Asp His Ala Phe Gly Asn 
        195                 200                 205             


Leu Ser Ser Leu Val Val Leu His Leu His Asn Asn Arg Ile His Ser 
    210                 215                 220                 


Leu Gly Lys Lys Cys Phe Asp Gly Leu His Ser Leu Glu Thr Leu Asp 
225                 230                 235                 240 


Leu Asn Tyr Asn Asn Leu Asp Glu Phe Pro Thr Ala Ile Lys Thr Leu 
                245                 250                 255     


Ser Asn Leu Lys Glu Leu Gly Phe His Ser Asn Asn Ile Arg Ser Ile 
            260                 265                 270         


Pro Glu Arg Ala Phe Val Gly Asn Pro Ser Leu Ile Thr Ile His Phe 
        275                 280                 285             


Tyr Asp Asn Pro Ile Gln Phe Val Gly Ile Ser Ala Phe Gln His Leu 
    290                 295                 300                 


Pro Glu Leu Arg Thr Leu Thr Leu Asn Gly Ala Ser Gln Ile Thr Glu 
305                 310                 315                 320 


Phe Pro Asp Leu Thr Gly Thr Ala Thr Leu Glu Ser Leu Thr Leu Thr 
                325                 330                 335     


Gly Ala Lys Ile Ser Ser Leu Pro Gln Thr Val Cys Asp Gln Leu Pro 
            340                 345                 350         


Asn Leu Gln Val Leu Asp Leu Ser Tyr Asn Leu Leu Glu Asp Leu Pro 
        355                 360                 365             


Ser Leu Ser Gly Cys Gln Lys Leu Gln Lys Ile Asp Leu Arg His Asn 
    370                 375                 380                 


Glu Ile Tyr Glu Ile Lys Gly Gly Thr Phe Gln Gln Leu Phe Asn Leu 
385                 390                 395                 400 


Arg Ser Leu Asn Leu Ala Arg Asn Lys Ile Ala Ile Ile His Pro Asn 
                405                 410                 415     


Ala Phe Ser Thr Leu Pro Ser Leu Ile Lys Leu Asp Leu Ser Ser Asn 
            420                 425                 430         


Leu Leu Ser Ser Phe Pro Val Thr Gly Leu His Gly Leu Thr His Leu 
        435                 440                 445             


Lys Leu Thr Gly Asn Arg Ala Leu Gln Ser Leu Ile Pro Ser Ala Asn 
    450                 455                 460                 


Phe Pro Glu Leu Lys Ile Ile Glu Met Pro Tyr Ala Tyr Gln Cys Cys 
465                 470                 475                 480 


Ala Phe Gly Gly Cys Glu Asn Val Tyr Lys Ile Pro Asn Gln Trp Asn 
                485                 490                 495     


Lys Asp Asp Ser Ser Ser Val Asp Asp Leu Arg Lys Lys Asp Ala Gly 
            500                 505                 510         


Leu Phe Gln Val Gln Asp Glu Arg Asp Leu Glu Asp Phe Leu Leu Asp 
        515                 520                 525             


Phe Glu Glu Asp Leu Lys Val Leu His Ser Val Gln Cys Ser Pro Pro 
    530                 535                 540                 


Pro Gly Pro Phe Lys Pro Cys Glu His Leu Phe Gly Ser Trp Leu Ile 
545                 550                 555                 560 


Arg Ile Gly Val Trp Thr Thr Ala Val Leu Ala Leu Ser Cys Asn Ala 
                565                 570                 575     


Leu Val Ala Phe Thr Val Phe Arg Thr Pro Leu Tyr Ile Ser Ser Ile 
            580                 585                 590         


Lys Leu Leu Ile Gly Val Ile Ala Val Val Asp Ile Leu Met Gly Val 
        595                 600                 605             


Ser Ser Ala Ile Leu Ala Val Val Asp Thr Phe Thr Phe Gly Ser Phe 
    610                 615                 620                 


Ala Gln His Gly Ala Trp Trp Glu Gly Gly Ile Gly Cys Gln Ile Val 
625                 630                 635                 640 


Gly Phe Leu Ser Ile Phe Ala Ser Glu Ser Ser Val Phe Leu Leu Thr 
                645                 650                 655     


Leu Ala Ala Leu Glu Arg Gly Phe Ser Val Lys Cys Ser Ser Lys Phe 
            660                 665                 670         


Glu Met Lys Ala Pro Leu Ser Ser Leu Lys Ala Ile Ile Leu Leu Cys 
        675                 680                 685             


Val Leu Leu Ala Leu Thr Ile Ala Thr Val Pro Leu Leu Gly Gly Ser 
    690                 695                 700                 


Glu Tyr Asn Ala Ser Pro Leu Cys Leu Pro Leu Pro Phe Gly Glu Pro 
705                 710                 715                 720 


Ser Thr Thr Gly Tyr Met Val Ala Leu Val Leu Leu Asn Ser Leu Cys 
                725                 730                 735     


Phe Leu Ile Met Thr Ile Ala Tyr Thr Arg Leu Tyr Cys Ser Leu Glu 
            740                 745                 750         


Lys Gly Glu Leu Glu Asn Leu Trp Asp Cys Ser Met Val Lys His Thr 
        755                 760                 765             


Ala Leu Leu Leu Phe Thr Asn Cys Ile Leu Tyr Cys Pro Val Ala Phe 
    770                 775                 780                 


Leu Ser Phe Ser Ser Leu Leu Asn Leu Thr Phe Ile Ser Pro Glu Val 
785                 790                 795                 800 


Ile Lys Phe Ile Leu Leu Val Ile Val Pro Leu Pro Ala Cys Leu Asn 
                805                 810                 815     


Pro Leu Leu Tyr Ile Val Phe Asn Pro His Phe Lys Glu Asp Met Gly 
            820                 825                 830         


Ser Leu Gly Lys Gln Thr Arg Phe Trp Thr Arg Ala Lys His Pro Ser 
        835                 840                 845             


Leu Leu Ser Ile Asn Ser Asp Asp Val Glu Lys Arg Ser Cys Asp Ser 
    850                 855                 860                 


Thr Gln Ala Leu Val Ser Phe Thr His Ala Ser Ile Ala Tyr Asp Leu 
865                 870                 875                 880 


Pro Ser Asp Ser Gly Ser Ser Pro Ala Tyr Pro Met Thr Glu Ser Cys 
                885                 890                 895     


His Leu Ser Ser Val Ala Phe Val Pro Cys Leu 
            900                 905         


<210>  71
<211>  886
<212>  PRT
<213>  Rattus sp.


<220>
<221>  misc_feature
<223>  Rat LgR5 mature, without signal sequence; amino acids 22 to 907

<400>  71

Gly Ser Pro Pro Arg Pro Asp Thr Met Pro Arg Gly Cys Pro Ser Tyr 
1               5                   10                  15      


Cys His Cys Glu Leu Asp Gly Arg Met Leu Leu Arg Val Asp Cys Ser 
            20                  25                  30          


Asp Leu Gly Leu Ser Glu Leu Pro Ser Asn Leu Ser Val Phe Thr Ser 
        35                  40                  45              


Tyr Leu Asp Leu Ser Met Asn Asn Ile Ser Gln Leu Pro Ala Ser Leu 
    50                  55                  60                  


Leu His Arg Leu Arg Phe Leu Glu Glu Leu Arg Leu Ala Gly Asn Ala 
65                  70                  75                  80  


Leu Thr His Ile Pro Lys Gly Ala Phe Ala Gly Leu His Ser Leu Lys 
                85                  90                  95      


Val Leu Met Leu Gln Asn Asn Gln Leu Arg Gln Val Pro Glu Glu Ala 
            100                 105                 110         


Leu Gln Asn Leu Arg Ser Leu Gln Ser Leu Arg Leu Asp Ala Asn His 
        115                 120                 125             


Ile Ser Tyr Val Pro Pro Ser Cys Phe Ser Gly Leu His Ser Leu Arg 
    130                 135                 140                 


His Leu Trp Leu Asp Asp Asn Ala Leu Thr Asp Val Pro Val Gln Ala 
145                 150                 155                 160 


Phe Arg Ser Leu Ser Ala Leu Gln Ala Met Thr Leu Ala Leu Asn Lys 
                165                 170                 175     


Ile His His Ile Ala Asp His Ala Phe Gly Asn Leu Ser Ser Leu Val 
            180                 185                 190         


Val Leu His Leu His Asn Asn Arg Ile His Ser Leu Gly Lys Lys Cys 
        195                 200                 205             


Phe Asp Gly Leu His Ser Leu Glu Thr Leu Asp Leu Asn Tyr Asn Asn 
    210                 215                 220                 


Leu Asp Glu Phe Pro Thr Ala Ile Lys Thr Leu Ser Asn Leu Lys Glu 
225                 230                 235                 240 


Leu Gly Phe His Ser Asn Asn Ile Arg Ser Ile Pro Glu Arg Ala Phe 
                245                 250                 255     


Val Gly Asn Pro Ser Leu Ile Thr Ile His Phe Tyr Asp Asn Pro Ile 
            260                 265                 270         


Gln Phe Val Gly Ile Ser Ala Phe Gln His Leu Pro Glu Leu Arg Thr 
        275                 280                 285             


Leu Thr Leu Asn Gly Ala Ser Gln Ile Thr Glu Phe Pro Asp Leu Thr 
    290                 295                 300                 


Gly Thr Ala Thr Leu Glu Ser Leu Thr Leu Thr Gly Ala Lys Ile Ser 
305                 310                 315                 320 


Ser Leu Pro Gln Thr Val Cys Asp Gln Leu Pro Asn Leu Gln Val Leu 
                325                 330                 335     


Asp Leu Ser Tyr Asn Leu Leu Glu Asp Leu Pro Ser Leu Ser Gly Cys 
            340                 345                 350         


Gln Lys Leu Gln Lys Ile Asp Leu Arg His Asn Glu Ile Tyr Glu Ile 
        355                 360                 365             


Lys Gly Gly Thr Phe Gln Gln Leu Phe Asn Leu Arg Ser Leu Asn Leu 
    370                 375                 380                 


Ala Arg Asn Lys Ile Ala Ile Ile His Pro Asn Ala Phe Ser Thr Leu 
385                 390                 395                 400 


Pro Ser Leu Ile Lys Leu Asp Leu Ser Ser Asn Leu Leu Ser Ser Phe 
                405                 410                 415     


Pro Val Thr Gly Leu His Gly Leu Thr His Leu Lys Leu Thr Gly Asn 
            420                 425                 430         


Arg Ala Leu Gln Ser Leu Ile Pro Ser Ala Asn Phe Pro Glu Leu Lys 
        435                 440                 445             


Ile Ile Glu Met Pro Tyr Ala Tyr Gln Cys Cys Ala Phe Gly Gly Cys 
    450                 455                 460                 


Glu Asn Val Tyr Lys Ile Pro Asn Gln Trp Asn Lys Asp Asp Ser Ser 
465                 470                 475                 480 


Ser Val Asp Asp Leu Arg Lys Lys Asp Ala Gly Leu Phe Gln Val Gln 
                485                 490                 495     


Asp Glu Arg Asp Leu Glu Asp Phe Leu Leu Asp Phe Glu Glu Asp Leu 
            500                 505                 510         


Lys Val Leu His Ser Val Gln Cys Ser Pro Pro Pro Gly Pro Phe Lys 
        515                 520                 525             


Pro Cys Glu His Leu Phe Gly Ser Trp Leu Ile Arg Ile Gly Val Trp 
    530                 535                 540                 


Thr Thr Ala Val Leu Ala Leu Ser Cys Asn Ala Leu Val Ala Phe Thr 
545                 550                 555                 560 


Val Phe Arg Thr Pro Leu Tyr Ile Ser Ser Ile Lys Leu Leu Ile Gly 
                565                 570                 575     


Val Ile Ala Val Val Asp Ile Leu Met Gly Val Ser Ser Ala Ile Leu 
            580                 585                 590         


Ala Val Val Asp Thr Phe Thr Phe Gly Ser Phe Ala Gln His Gly Ala 
        595                 600                 605             


Trp Trp Glu Gly Gly Ile Gly Cys Gln Ile Val Gly Phe Leu Ser Ile 
    610                 615                 620                 


Phe Ala Ser Glu Ser Ser Val Phe Leu Leu Thr Leu Ala Ala Leu Glu 
625                 630                 635                 640 


Arg Gly Phe Ser Val Lys Cys Ser Ser Lys Phe Glu Met Lys Ala Pro 
                645                 650                 655     


Leu Ser Ser Leu Lys Ala Ile Ile Leu Leu Cys Val Leu Leu Ala Leu 
            660                 665                 670         


Thr Ile Ala Thr Val Pro Leu Leu Gly Gly Ser Glu Tyr Asn Ala Ser 
        675                 680                 685             


Pro Leu Cys Leu Pro Leu Pro Phe Gly Glu Pro Ser Thr Thr Gly Tyr 
    690                 695                 700                 


Met Val Ala Leu Val Leu Leu Asn Ser Leu Cys Phe Leu Ile Met Thr 
705                 710                 715                 720 


Ile Ala Tyr Thr Arg Leu Tyr Cys Ser Leu Glu Lys Gly Glu Leu Glu 
                725                 730                 735     


Asn Leu Trp Asp Cys Ser Met Val Lys His Thr Ala Leu Leu Leu Phe 
            740                 745                 750         


Thr Asn Cys Ile Leu Tyr Cys Pro Val Ala Phe Leu Ser Phe Ser Ser 
        755                 760                 765             


Leu Leu Asn Leu Thr Phe Ile Ser Pro Glu Val Ile Lys Phe Ile Leu 
    770                 775                 780                 


Leu Val Ile Val Pro Leu Pro Ala Cys Leu Asn Pro Leu Leu Tyr Ile 
785                 790                 795                 800 


Val Phe Asn Pro His Phe Lys Glu Asp Met Gly Ser Leu Gly Lys Gln 
                805                 810                 815     


Thr Arg Phe Trp Thr Arg Ala Lys His Pro Ser Leu Leu Ser Ile Asn 
            820                 825                 830         


Ser Asp Asp Val Glu Lys Arg Ser Cys Asp Ser Thr Gln Ala Leu Val 
        835                 840                 845             


Ser Phe Thr His Ala Ser Ile Ala Tyr Asp Leu Pro Ser Asp Ser Gly 
    850                 855                 860                 


Ser Ser Pro Ala Tyr Pro Met Thr Glu Ser Cys His Leu Ser Ser Val 
865                 870                 875                 880 


Ala Phe Val Pro Cys Leu 
                885     


<210>  72
<211>  907
<212>  PRT
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  Mouse LgR5 precursor; LGR5_mouse NP_034325; signal sequence = 
       amino acids 1-21

<400>  72

Met Asp Thr Ser Cys Val His Met Leu Leu Ser Leu Leu Ala Leu Leu 
1               5                   10                  15      


Gln Leu Val Ala Ala Gly Ser Ser Pro Gly Pro Asp Ala Ile Pro Arg 
            20                  25                  30          


Gly Cys Pro Ser His Cys His Cys Glu Leu Asp Gly Arg Met Leu Leu 
        35                  40                  45              


Arg Val Asp Cys Ser Asp Leu Gly Leu Ser Glu Leu Pro Ser Asn Leu 
    50                  55                  60                  


Ser Val Phe Thr Ser Tyr Leu Asp Leu Ser Met Asn Asn Ile Ser Gln 
65                  70                  75                  80  


Leu Pro Ala Ser Leu Leu His Arg Leu Cys Phe Leu Glu Glu Leu Arg 
                85                  90                  95      


Leu Ala Gly Asn Ala Leu Thr His Ile Pro Lys Gly Ala Phe Thr Gly 
            100                 105                 110         


Leu His Ser Leu Lys Val Leu Met Leu Gln Asn Asn Gln Leu Arg Gln 
        115                 120                 125             


Val Pro Glu Glu Ala Leu Gln Asn Leu Arg Ser Leu Gln Ser Leu Arg 
    130                 135                 140                 


Leu Asp Ala Asn His Ile Ser Tyr Val Pro Pro Ser Cys Phe Ser Gly 
145                 150                 155                 160 


Leu His Ser Leu Arg His Leu Trp Leu Asp Asp Asn Ala Leu Thr Asp 
                165                 170                 175     


Val Pro Val Gln Ala Phe Arg Ser Leu Ser Ala Leu Gln Ala Met Thr 
            180                 185                 190         


Leu Ala Leu Asn Lys Ile His His Ile Ala Asp Tyr Ala Phe Gly Asn 
        195                 200                 205             


Leu Ser Ser Leu Val Val Leu His Leu His Asn Asn Arg Ile His Ser 
    210                 215                 220                 


Leu Gly Lys Lys Cys Phe Asp Gly Leu His Ser Leu Glu Thr Leu Asp 
225                 230                 235                 240 


Leu Asn Tyr Asn Asn Leu Asp Glu Phe Pro Thr Ala Ile Lys Thr Leu 
                245                 250                 255     


Ser Asn Leu Lys Glu Leu Gly Phe His Ser Asn Asn Ile Arg Ser Ile 
            260                 265                 270         


Pro Glu Arg Ala Phe Val Gly Asn Pro Ser Leu Ile Thr Ile His Phe 
        275                 280                 285             


Tyr Asp Asn Pro Ile Gln Phe Val Gly Val Ser Ala Phe Gln His Leu 
    290                 295                 300                 


Pro Glu Leu Arg Thr Leu Thr Leu Asn Gly Ala Ser His Ile Thr Glu 
305                 310                 315                 320 


Phe Pro His Leu Thr Gly Thr Ala Thr Leu Glu Ser Leu Thr Leu Thr 
                325                 330                 335     


Gly Ala Lys Ile Ser Ser Leu Pro Gln Ala Val Cys Asp Gln Leu Pro 
            340                 345                 350         


Asn Leu Gln Val Leu Asp Leu Ser Tyr Asn Leu Leu Glu Asp Leu Pro 
        355                 360                 365             


Ser Leu Ser Gly Cys Gln Lys Leu Gln Lys Ile Asp Leu Arg His Asn 
    370                 375                 380                 


Glu Ile Tyr Glu Ile Lys Gly Ser Thr Phe Gln Gln Leu Phe Asn Leu 
385                 390                 395                 400 


Arg Ser Leu Asn Leu Ala Trp Asn Lys Ile Ala Ile Ile His Pro Asn 
                405                 410                 415     


Ala Phe Ser Thr Leu Pro Ser Leu Ile Lys Leu Asp Leu Ser Ser Asn 
            420                 425                 430         


Leu Leu Ser Ser Phe Pro Val Thr Gly Leu His Gly Leu Thr His Leu 
        435                 440                 445             


Lys Leu Thr Gly Asn Arg Ala Leu Gln Ser Leu Ile Pro Ser Ala Asn 
    450                 455                 460                 


Phe Pro Glu Leu Lys Ile Ile Glu Met Pro Ser Ala Tyr Gln Cys Cys 
465                 470                 475                 480 


Ala Phe Gly Gly Cys Glu Asn Val Tyr Lys Ile Ser Asn Gln Trp Asn 
                485                 490                 495     


Lys Asp Asp Gly Asn Ser Val Asp Asp Leu His Lys Lys Asp Ala Gly 
            500                 505                 510         


Leu Phe Gln Val Gln Asp Glu Arg Asp Leu Glu Asp Phe Leu Leu Asp 
        515                 520                 525             


Phe Glu Glu Asp Leu Lys Ala Leu His Ser Val Gln Cys Ser Pro Ser 
    530                 535                 540                 


Pro Gly Pro Phe Lys Pro Cys Glu His Leu Phe Gly Ser Trp Leu Ile 
545                 550                 555                 560 


Arg Ile Gly Val Trp Thr Thr Ala Val Leu Ala Leu Ser Cys Asn Ala 
                565                 570                 575     


Leu Val Ala Leu Thr Val Phe Arg Thr Pro Leu Tyr Ile Ser Ser Ile 
            580                 585                 590         


Lys Leu Leu Ile Gly Val Ile Ala Val Val Asp Ile Leu Met Gly Val 
        595                 600                 605             


Ser Ser Ala Val Leu Ala Ala Val Asp Ala Phe Thr Phe Gly Arg Phe 
    610                 615                 620                 


Ala Gln His Gly Ala Trp Trp Glu Asp Gly Ile Gly Cys Gln Ile Val 
625                 630                 635                 640 


Gly Phe Leu Ser Ile Phe Ala Ser Glu Ser Ser Ile Phe Leu Leu Thr 
                645                 650                 655     


Leu Ala Ala Leu Glu Arg Gly Phe Ser Val Lys Cys Ser Ser Lys Phe 
            660                 665                 670         


Glu Val Lys Ala Pro Leu Phe Ser Leu Arg Ala Ile Val Leu Leu Cys 
        675                 680                 685             


Val Leu Leu Ala Leu Thr Ile Ala Thr Ile Pro Leu Leu Gly Gly Ser 
    690                 695                 700                 


Lys Tyr Asn Ala Ser Pro Leu Cys Leu Pro Leu Pro Phe Gly Glu Pro 
705                 710                 715                 720 


Ser Thr Thr Gly Tyr Met Val Ala Leu Val Leu Leu Asn Ser Leu Cys 
                725                 730                 735     


Phe Leu Ile Met Thr Ile Ala Tyr Thr Lys Leu Tyr Cys Ser Leu Glu 
            740                 745                 750         


Lys Gly Glu Leu Glu Asn Leu Trp Asp Cys Ser Met Val Lys His Ile 
        755                 760                 765             


Ala Leu Leu Leu Phe Ala Asn Cys Ile Leu Tyr Cys Pro Val Ala Phe 
    770                 775                 780                 


Leu Ser Phe Ser Ser Leu Leu Asn Leu Thr Phe Ile Ser Pro Asp Val 
785                 790                 795                 800 


Ile Lys Phe Ile Leu Leu Val Ile Val Pro Leu Pro Ser Cys Leu Asn 
                805                 810                 815     


Pro Leu Leu Tyr Ile Val Phe Asn Pro His Phe Lys Glu Asp Met Gly 
            820                 825                 830         


Ser Leu Gly Lys His Thr Arg Phe Trp Met Arg Ser Lys His Ala Ser 
        835                 840                 845             


Leu Leu Ser Ile Asn Ser Asp Asp Val Glu Lys Arg Ser Cys Glu Ser 
    850                 855                 860                 


Thr Gln Ala Leu Val Ser Phe Thr His Ala Ser Ile Ala Tyr Asp Leu 
865                 870                 875                 880 


Pro Ser Thr Ser Gly Ala Ser Pro Ala Tyr Pro Met Thr Glu Ser Cys 
                885                 890                 895     


His Leu Ser Ser Val Ala Phe Val Pro Cys Leu 
            900                 905         


<210>  73
<211>  886
<212>  PRT
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  Mouse LgR5 mature, without signal sequence; amino acids 22 to 907

<400>  73

Gly Ser Ser Pro Gly Pro Asp Ala Ile Pro Arg Gly Cys Pro Ser His 
1               5                   10                  15      


Cys His Cys Glu Leu Asp Gly Arg Met Leu Leu Arg Val Asp Cys Ser 
            20                  25                  30          


Asp Leu Gly Leu Ser Glu Leu Pro Ser Asn Leu Ser Val Phe Thr Ser 
        35                  40                  45              


Tyr Leu Asp Leu Ser Met Asn Asn Ile Ser Gln Leu Pro Ala Ser Leu 
    50                  55                  60                  


Leu His Arg Leu Cys Phe Leu Glu Glu Leu Arg Leu Ala Gly Asn Ala 
65                  70                  75                  80  


Leu Thr His Ile Pro Lys Gly Ala Phe Thr Gly Leu His Ser Leu Lys 
                85                  90                  95      


Val Leu Met Leu Gln Asn Asn Gln Leu Arg Gln Val Pro Glu Glu Ala 
            100                 105                 110         


Leu Gln Asn Leu Arg Ser Leu Gln Ser Leu Arg Leu Asp Ala Asn His 
        115                 120                 125             


Ile Ser Tyr Val Pro Pro Ser Cys Phe Ser Gly Leu His Ser Leu Arg 
    130                 135                 140                 


His Leu Trp Leu Asp Asp Asn Ala Leu Thr Asp Val Pro Val Gln Ala 
145                 150                 155                 160 


Phe Arg Ser Leu Ser Ala Leu Gln Ala Met Thr Leu Ala Leu Asn Lys 
                165                 170                 175     


Ile His His Ile Ala Asp Tyr Ala Phe Gly Asn Leu Ser Ser Leu Val 
            180                 185                 190         


Val Leu His Leu His Asn Asn Arg Ile His Ser Leu Gly Lys Lys Cys 
        195                 200                 205             


Phe Asp Gly Leu His Ser Leu Glu Thr Leu Asp Leu Asn Tyr Asn Asn 
    210                 215                 220                 


Leu Asp Glu Phe Pro Thr Ala Ile Lys Thr Leu Ser Asn Leu Lys Glu 
225                 230                 235                 240 


Leu Gly Phe His Ser Asn Asn Ile Arg Ser Ile Pro Glu Arg Ala Phe 
                245                 250                 255     


Val Gly Asn Pro Ser Leu Ile Thr Ile His Phe Tyr Asp Asn Pro Ile 
            260                 265                 270         


Gln Phe Val Gly Val Ser Ala Phe Gln His Leu Pro Glu Leu Arg Thr 
        275                 280                 285             


Leu Thr Leu Asn Gly Ala Ser His Ile Thr Glu Phe Pro His Leu Thr 
    290                 295                 300                 


Gly Thr Ala Thr Leu Glu Ser Leu Thr Leu Thr Gly Ala Lys Ile Ser 
305                 310                 315                 320 


Ser Leu Pro Gln Ala Val Cys Asp Gln Leu Pro Asn Leu Gln Val Leu 
                325                 330                 335     


Asp Leu Ser Tyr Asn Leu Leu Glu Asp Leu Pro Ser Leu Ser Gly Cys 
            340                 345                 350         


Gln Lys Leu Gln Lys Ile Asp Leu Arg His Asn Glu Ile Tyr Glu Ile 
        355                 360                 365             


Lys Gly Ser Thr Phe Gln Gln Leu Phe Asn Leu Arg Ser Leu Asn Leu 
    370                 375                 380                 


Ala Trp Asn Lys Ile Ala Ile Ile His Pro Asn Ala Phe Ser Thr Leu 
385                 390                 395                 400 


Pro Ser Leu Ile Lys Leu Asp Leu Ser Ser Asn Leu Leu Ser Ser Phe 
                405                 410                 415     


Pro Val Thr Gly Leu His Gly Leu Thr His Leu Lys Leu Thr Gly Asn 
            420                 425                 430         


Arg Ala Leu Gln Ser Leu Ile Pro Ser Ala Asn Phe Pro Glu Leu Lys 
        435                 440                 445             


Ile Ile Glu Met Pro Ser Ala Tyr Gln Cys Cys Ala Phe Gly Gly Cys 
    450                 455                 460                 


Glu Asn Val Tyr Lys Ile Ser Asn Gln Trp Asn Lys Asp Asp Gly Asn 
465                 470                 475                 480 


Ser Val Asp Asp Leu His Lys Lys Asp Ala Gly Leu Phe Gln Val Gln 
                485                 490                 495     


Asp Glu Arg Asp Leu Glu Asp Phe Leu Leu Asp Phe Glu Glu Asp Leu 
            500                 505                 510         


Lys Ala Leu His Ser Val Gln Cys Ser Pro Ser Pro Gly Pro Phe Lys 
        515                 520                 525             


Pro Cys Glu His Leu Phe Gly Ser Trp Leu Ile Arg Ile Gly Val Trp 
    530                 535                 540                 


Thr Thr Ala Val Leu Ala Leu Ser Cys Asn Ala Leu Val Ala Leu Thr 
545                 550                 555                 560 


Val Phe Arg Thr Pro Leu Tyr Ile Ser Ser Ile Lys Leu Leu Ile Gly 
                565                 570                 575     


Val Ile Ala Val Val Asp Ile Leu Met Gly Val Ser Ser Ala Val Leu 
            580                 585                 590         


Ala Ala Val Asp Ala Phe Thr Phe Gly Arg Phe Ala Gln His Gly Ala 
        595                 600                 605             


Trp Trp Glu Asp Gly Ile Gly Cys Gln Ile Val Gly Phe Leu Ser Ile 
    610                 615                 620                 


Phe Ala Ser Glu Ser Ser Ile Phe Leu Leu Thr Leu Ala Ala Leu Glu 
625                 630                 635                 640 


Arg Gly Phe Ser Val Lys Cys Ser Ser Lys Phe Glu Val Lys Ala Pro 
                645                 650                 655     


Leu Phe Ser Leu Arg Ala Ile Val Leu Leu Cys Val Leu Leu Ala Leu 
            660                 665                 670         


Thr Ile Ala Thr Ile Pro Leu Leu Gly Gly Ser Lys Tyr Asn Ala Ser 
        675                 680                 685             


Pro Leu Cys Leu Pro Leu Pro Phe Gly Glu Pro Ser Thr Thr Gly Tyr 
    690                 695                 700                 


Met Val Ala Leu Val Leu Leu Asn Ser Leu Cys Phe Leu Ile Met Thr 
705                 710                 715                 720 


Ile Ala Tyr Thr Lys Leu Tyr Cys Ser Leu Glu Lys Gly Glu Leu Glu 
                725                 730                 735     


Asn Leu Trp Asp Cys Ser Met Val Lys His Ile Ala Leu Leu Leu Phe 
            740                 745                 750         


Ala Asn Cys Ile Leu Tyr Cys Pro Val Ala Phe Leu Ser Phe Ser Ser 
        755                 760                 765             


Leu Leu Asn Leu Thr Phe Ile Ser Pro Asp Val Ile Lys Phe Ile Leu 
    770                 775                 780                 


Leu Val Ile Val Pro Leu Pro Ser Cys Leu Asn Pro Leu Leu Tyr Ile 
785                 790                 795                 800 


Val Phe Asn Pro His Phe Lys Glu Asp Met Gly Ser Leu Gly Lys His 
                805                 810                 815     


Thr Arg Phe Trp Met Arg Ser Lys His Ala Ser Leu Leu Ser Ile Asn 
            820                 825                 830         


Ser Asp Asp Val Glu Lys Arg Ser Cys Glu Ser Thr Gln Ala Leu Val 
        835                 840                 845             


Ser Phe Thr His Ala Ser Ile Ala Tyr Asp Leu Pro Ser Thr Ser Gly 
    850                 855                 860                 


Ala Ser Pro Ala Tyr Pro Met Thr Glu Ser Cys His Leu Ser Ser Val 
865                 870                 875                 880 


Ala Phe Val Pro Cys Leu 
                885     


<210>  74
<211>  218
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v2 V205C cysteine engineered light chain (Igk)

<400>  74

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ala Ser Glu Ser Val Asp Asn Tyr 
            20                  25                  30          


Gly Asn Ser Phe Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Leu Ala Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Asn Tyr 
                85                  90                  95      


Glu Asp Pro Phe Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110         


Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
        115                 120                 125             


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
    130                 135                 140                 


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
145                 150                 155                 160 


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
                165                 170                 175     


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
            180                 185                 190         


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
        195                 200                 205             


Cys Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215             


<210>  75
<211>  448
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v2 A118C cysteine engineered heavy chain (IgG1)

<400>  75

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ser Ala Tyr 
            20                  25                  30          


Trp Ile Glu Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Leu Pro Gly Ser Asp Ser Thr Asp Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Val Arg Ala Thr Phe Thr Ser Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Leu Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly His Tyr Gly Ser Leu Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Cys Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr 
    210                 215                 220                 


His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 
225                 230                 235                 240 


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
                245                 250                 255     


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
            260                 265                 270         


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
        275                 280                 285             


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
    290                 295                 300                 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
305                 310                 315                 320 


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 
                325                 330                 335     


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
            340                 345                 350         


Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
        355                 360                 365             


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
    370                 375                 380                 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
385                 390                 395                 400 


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
                405                 410                 415     


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
            420                 425                 430         


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445             


<210>  76
<211>  448
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu8E11.v2 S400C cysteine engineered heavy chain (IgG1)

<400>  76

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ser Ala Tyr 
            20                  25                  30          


Trp Ile Glu Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Leu Pro Gly Ser Asp Ser Thr Asp Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Val Arg Ala Thr Phe Thr Ser Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Leu Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly His Tyr Gly Ser Leu Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr 
    210                 215                 220                 


His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 
225                 230                 235                 240 


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
                245                 250                 255     


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
            260                 265                 270         


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
        275                 280                 285             


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
    290                 295                 300                 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
305                 310                 315                 320 


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 
                325                 330                 335     


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
            340                 345                 350         


Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
        355                 360                 365             


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
    370                 375                 380                 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
385                 390                 395                 400 


Cys Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
                405                 410                 415     


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
            420                 425                 430         


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445             


<210>  77
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YW353 V205C cysteine engineered light chain (Igk)

<400>  77

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Cys Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  78
<211>  446
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YW353 A118C cysteine engineered heavy chain (IgG1)

<400>  78

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Ser Tyr 
            20                  25                  30          


Ser Ile Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Glu Ile Tyr Pro Pro Gly Gly Tyr Thr Asp Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Ala Arg Leu Phe Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser Cys Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala 
        115                 120                 125             


Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu 
    130                 135                 140                 


Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly 
145                 150                 155                 160 


Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser 
                165                 170                 175     


Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu 
            180                 185                 190         


Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr 
        195                 200                 205             


Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr 
    210                 215                 220                 


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
225                 230                 235                 240 


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
                245                 250                 255     


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
            260                 265                 270         


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
        275                 280                 285             


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
    290                 295                 300                 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
305                 310                 315                 320 


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
                325                 330                 335     


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
            340                 345                 350         


Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
        355                 360                 365             


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
    370                 375                 380                 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
385                 390                 395                 400 


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
                405                 410                 415     


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
            420                 425                 430         


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445     


<210>  79
<211>  446
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: YW353 S400C cysteine engineered heavy chain (IgG1)

<400>  79

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Ser Tyr 
            20                  25                  30          


Ser Ile Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Glu Ile Tyr Pro Pro Gly Gly Tyr Thr Asp Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Ala Arg Leu Phe Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala 
        115                 120                 125             


Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu 
    130                 135                 140                 


Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly 
145                 150                 155                 160 


Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser 
                165                 170                 175     


Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu 
            180                 185                 190         


Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr 
        195                 200                 205             


Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr 
    210                 215                 220                 


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
225                 230                 235                 240 


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
                245                 250                 255     


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
            260                 265                 270         


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
        275                 280                 285             


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
    290                 295                 300                 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
305                 310                 315                 320 


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
                325                 330                 335     


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
            340                 345                 350         


Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
        355                 360                 365             


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
    370                 375                 380                 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Cys Asp 
385                 390                 395                 400 


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
                405                 410                 415     


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
            420                 425                 430         


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445     


<210>  80
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: V205C cysteine engineered light chain constant region 
       (Igk)

<400>  80

Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
1               5                   10                  15      


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
            20                  25                  30          


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
        35                  40                  45              


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
    50                  55                  60                  


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
65                  70                  75                  80  


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
                85                  90                  95      


Cys Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105     


<210>  81
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: A118C cysteine engineered heavy chain constant region 
       (IgG1)

<400>  81

Cys Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  82
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: S400C cysteine engineered heavy chain constant region 
       (IgG1)

<400>  82

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Cys Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  83
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: primer

<400>  83
accaactgca tcctaaactg                                                   20


<210>  84
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: primer

<400>  84
accgagtttc acctcagctc                                                   20


<210>  85
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: primer

<400>  85
acattgccct gttgctcttc                                                   20


<210>  86
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: primer

<400>  86
actgctctga tatactcaat c                                                 21


