                         SEQUENCE LISTING

<110>  Donovan, David M.
 
<120>  Enhanced Antimicrobial Lytic Activity of a Chimeric Ply187 
       Endolysin

<130>  0112.10

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  157
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage 187

<400>  1

Met Ala Leu Pro Lys Thr Gly Lys Pro Thr Ala Lys Gln Val Val Asp 
1               5                   10                  15      


Trp Ala Ile Asn Leu Ile Gly Ser Gly Val Asp Val Asp Gly Tyr Tyr 
            20                  25                  30          


Gly Arg Gln Cys Trp Asp Leu Pro Asn Tyr Ile Phe Asn Arg Tyr Trp 
        35                  40                  45              


Asn Phe Lys Thr Pro Gly Asn Ala Arg Asp Met Ala Trp Tyr Arg Tyr 
    50                  55                  60                  


Pro Glu Gly Phe Lys Val Phe Arg Asn Thr Ser Asp Phe Val Pro Lys 
65                  70                  75                  80  


Pro Gly Asp Ile Ala Val Trp Thr Gly Gly Asn Tyr Asn Trp Asn Thr 
                85                  90                  95      


Trp Gly His Thr Gly Ile Val Val Gly Pro Ser Thr Lys Ser Tyr Phe 
            100                 105                 110         


Tyr Ser Val Asp Gln Asn Trp Asn Asn Ser Asn Ser Tyr Val Gly Ser 
        115                 120                 125             


Pro Ala Ala Lys Ile Lys His Ser Tyr Phe Gly Val Thr His Phe Val 
    130                 135                 140                 


Arg Pro Ala Tyr Lys Ala Glu Pro Lys Pro Thr Pro Pro 
145                 150                 155         


<210>  2
<211>  156
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage 187

<400>  2

Ala Leu Pro Lys Thr Gly Lys Pro Thr Ala Lys Gln Val Val Asp Trp 
1               5                   10                  15      


Ala Ile Asn Leu Ile Gly Ser Gly Val Asp Val Asp Gly Tyr Tyr Gly 
            20                  25                  30          


Arg Gln Cys Trp Asp Leu Pro Asn Tyr Ile Phe Asn Arg Tyr Trp Asn 
        35                  40                  45              


Phe Lys Thr Pro Gly Asn Ala Arg Asp Met Ala Trp Tyr Arg Tyr Pro 
    50                  55                  60                  


Glu Gly Phe Lys Val Phe Arg Asn Thr Ser Asp Phe Val Pro Lys Pro 
65                  70                  75                  80  


Gly Asp Ile Ala Val Trp Thr Gly Gly Asn Tyr Asn Trp Asn Thr Trp 
                85                  90                  95      


Gly His Thr Gly Ile Val Val Gly Pro Ser Thr Lys Ser Tyr Phe Tyr 
            100                 105                 110         


Ser Val Asp Gln Asn Trp Asn Asn Ser Asn Ser Tyr Val Gly Ser Pro 
        115                 120                 125             


Ala Ala Lys Ile Lys His Ser Tyr Phe Gly Val Thr His Phe Val Arg 
    130                 135                 140                 


Pro Ala Tyr Lys Ala Glu Pro Lys Pro Thr Pro Pro 
145                 150                 155     


<210>  3
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Phage K

<400>  3

Ser Thr Pro Ala Thr Arg Pro Val Thr Gly Ser Trp Lys Lys Asn Gln 
1               5                   10                  15      


Tyr Gly Thr Trp Tyr Lys Pro Glu Asn Ala Thr Phe Val Asn Gly Asn 
            20                  25                  30          


Gln Pro Ile Val Thr Arg Ile Gly Ser Pro Phe Leu Asn Ala Pro Val 
        35                  40                  45              


Gly Gly Asn Leu Pro Ala Gly Ala Thr Ile Val Tyr Asp Glu Val Cys 
    50                  55                  60                  


Ile Gln Ala Gly His Ile Trp Ile Gly Tyr Asn Ala Tyr Asn Gly Asn 
65                  70                  75                  80  


Arg Val Tyr Cys Pro Val Arg Thr Cys Gln Gly Val Pro Pro Asn Gln 
                85                  90                  95      


Ile Pro Gly Val Ala Trp Gly Val Phe Lys 
            100                 105     


<210>  4
<211>  265
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Chemically Synthesized Fusion Protein

<400>  4

Met Ala Leu Pro Lys Thr Gly Lys Pro Thr Ala Lys Gln Val Val Asp 
1               5                   10                  15      


Trp Ala Ile Asn Leu Ile Gly Ser Gly Val Asp Val Asp Gly Tyr Tyr 
            20                  25                  30          


Gly Arg Gln Cys Trp Asp Leu Pro Asn Tyr Ile Phe Asn Arg Tyr Trp 
        35                  40                  45              


Asn Phe Lys Thr Pro Gly Asn Ala Arg Asp Met Ala Trp Tyr Arg Tyr 
    50                  55                  60                  


Pro Glu Gly Phe Lys Val Phe Arg Asn Thr Ser Asp Phe Val Pro Lys 
65                  70                  75                  80  


Pro Gly Asp Ile Ala Val Trp Thr Gly Gly Asn Tyr Asn Trp Asn Thr 
                85                  90                  95      


Trp Gly His Thr Gly Ile Val Val Gly Pro Ser Thr Lys Ser Tyr Phe 
            100                 105                 110         


Tyr Ser Val Asp Gln Asn Trp Asn Asn Ser Asn Ser Tyr Val Gly Ser 
        115                 120                 125             


Pro Ala Ala Lys Ile Lys His Ser Tyr Phe Gly Val Thr His Phe Val 
    130                 135                 140                 


Arg Pro Ala Tyr Lys Ala Glu Pro Lys Pro Thr Pro Pro Leu Glu Ser 
145                 150                 155                 160 


Thr Pro Ala Thr Arg Pro Val Thr Gly Ser Trp Lys Lys Asn Gln Tyr 
                165                 170                 175     


Gly Thr Trp Tyr Lys Pro Glu Asn Ala Thr Phe Val Asn Gly Asn Gln 
            180                 185                 190         


Pro Ile Val Thr Arg Ile Gly Ser Pro Phe Leu Asn Ala Pro Val Gly 
        195                 200                 205             


Gly Asn Leu Pro Ala Gly Ala Thr Ile Val Tyr Asp Glu Val Cys Ile 
    210                 215                 220                 


Gln Ala Gly His Ile Trp Ile Gly Tyr Asn Ala Tyr Asn Gly Asn Arg 
225                 230                 235                 240 


Val Tyr Cys Pro Val Arg Thr Cys Gln Gly Val Pro Pro Asn Gln Ile 
                245                 250                 255     


Pro Gly Val Ala Trp Gly Val Phe Lys 
            260                 265 


<210>  5
<211>  264
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Chemically Synthesized Fusion Protein

<400>  5

Ala Leu Pro Lys Thr Gly Lys Pro Thr Ala Lys Gln Val Val Asp Trp 
1               5                   10                  15      


Ala Ile Asn Leu Ile Gly Ser Gly Val Asp Val Asp Gly Tyr Tyr Gly 
            20                  25                  30          


Arg Gln Cys Trp Asp Leu Pro Asn Tyr Ile Phe Asn Arg Tyr Trp Asn 
        35                  40                  45              


Phe Lys Thr Pro Gly Asn Ala Arg Asp Met Ala Trp Tyr Arg Tyr Pro 
    50                  55                  60                  


Glu Gly Phe Lys Val Phe Arg Asn Thr Ser Asp Phe Val Pro Lys Pro 
65                  70                  75                  80  


Gly Asp Ile Ala Val Trp Thr Gly Gly Asn Tyr Asn Trp Asn Thr Trp 
                85                  90                  95      


Gly His Thr Gly Ile Val Val Gly Pro Ser Thr Lys Ser Tyr Phe Tyr 
            100                 105                 110         


Ser Val Asp Gln Asn Trp Asn Asn Ser Asn Ser Tyr Val Gly Ser Pro 
        115                 120                 125             


Ala Ala Lys Ile Lys His Ser Tyr Phe Gly Val Thr His Phe Val Arg 
    130                 135                 140                 


Pro Ala Tyr Lys Ala Glu Pro Lys Pro Thr Pro Pro Leu Glu Ser Thr 
145                 150                 155                 160 


Pro Ala Thr Arg Pro Val Thr Gly Ser Trp Lys Lys Asn Gln Tyr Gly 
                165                 170                 175     


Thr Trp Tyr Lys Pro Glu Asn Ala Thr Phe Val Asn Gly Asn Gln Pro 
            180                 185                 190         


Ile Val Thr Arg Ile Gly Ser Pro Phe Leu Asn Ala Pro Val Gly Gly 
        195                 200                 205             


Asn Leu Pro Ala Gly Ala Thr Ile Val Tyr Asp Glu Val Cys Ile Gln 
    210                 215                 220                 


Ala Gly His Ile Trp Ile Gly Tyr Asn Ala Tyr Asn Gly Asn Arg Val 
225                 230                 235                 240 


Tyr Cys Pro Val Arg Thr Cys Gln Gly Val Pro Pro Asn Gln Ile Pro 
                245                 250                 255     


Gly Val Ala Trp Gly Val Phe Lys 
            260                 


<210>  6
<211>  267
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Chemically Synthesized Fusion Protein

<400>  6

Met Ala Leu Pro Lys Thr Gly Lys Pro Thr Ala Lys Gln Val Val Asp 
1               5                   10                  15      


Trp Ala Ile Asn Leu Ile Gly Ser Gly Val Asp Val Asp Gly Tyr Tyr 
            20                  25                  30          


Gly Arg Gln Cys Trp Asp Leu Pro Asn Tyr Ile Phe Asn Arg Tyr Trp 
        35                  40                  45              


Asn Phe Lys Thr Pro Gly Asn Ala Arg Asp Met Ala Trp Tyr Arg Tyr 
    50                  55                  60                  


Pro Glu Gly Phe Lys Val Phe Arg Asn Thr Ser Asp Phe Val Pro Lys 
65                  70                  75                  80  


Pro Gly Asp Ile Ala Val Trp Thr Gly Gly Asn Tyr Asn Trp Asn Thr 
                85                  90                  95      


Trp Gly His Thr Gly Ile Val Val Gly Pro Ser Thr Lys Ser Tyr Phe 
            100                 105                 110         


Tyr Ser Val Asp Gln Asn Trp Asn Asn Ser Asn Ser Tyr Val Gly Ser 
        115                 120                 125             


Pro Ala Ala Lys Ile Lys His Ser Tyr Phe Gly Val Thr His Phe Val 
    130                 135                 140                 


Arg Pro Ala Tyr Lys Ala Glu Pro Lys Pro Thr Pro Pro Leu Glu Ser 
145                 150                 155                 160 


Thr Pro Ala Thr Arg Pro Val Thr Gly Ser Trp Lys Lys Asn Gln Tyr 
                165                 170                 175     


Gly Thr Trp Tyr Lys Pro Glu Asn Ala Thr Phe Val Asn Gly Asn Gln 
            180                 185                 190         


Pro Ile Val Thr Arg Ile Gly Ser Pro Phe Leu Asn Ala Pro Val Gly 
        195                 200                 205             


Gly Asn Leu Pro Ala Gly Ala Thr Ile Val Tyr Asp Glu Val Cys Ile 
    210                 215                 220                 


Gln Ala Gly His Ile Trp Ile Gly Tyr Asn Ala Tyr Asn Gly Asn Arg 
225                 230                 235                 240 


Val Tyr Cys Pro Val Arg Thr Cys Gln Gly Val Pro Pro Asn Gln Ile 
                245                 250                 255     


Pro Gly Val Ala Trp Gly Val Phe Lys Val Glu 
            260                 265         


<210>  7
<211>  266
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Chemically Synthesized Fusion Protein

<400>  7

Ala Leu Pro Lys Thr Gly Lys Pro Thr Ala Lys Gln Val Val Asp Trp 
1               5                   10                  15      


Ala Ile Asn Leu Ile Gly Ser Gly Val Asp Val Asp Gly Tyr Tyr Gly 
            20                  25                  30          


Arg Gln Cys Trp Asp Leu Pro Asn Tyr Ile Phe Asn Arg Tyr Trp Asn 
        35                  40                  45              


Phe Lys Thr Pro Gly Asn Ala Arg Asp Met Ala Trp Tyr Arg Tyr Pro 
    50                  55                  60                  


Glu Gly Phe Lys Val Phe Arg Asn Thr Ser Asp Phe Val Pro Lys Pro 
65                  70                  75                  80  


Gly Asp Ile Ala Val Trp Thr Gly Gly Asn Tyr Asn Trp Asn Thr Trp 
                85                  90                  95      


Gly His Thr Gly Ile Val Val Gly Pro Ser Thr Lys Ser Tyr Phe Tyr 
            100                 105                 110         


Ser Val Asp Gln Asn Trp Asn Asn Ser Asn Ser Tyr Val Gly Ser Pro 
        115                 120                 125             


Ala Ala Lys Ile Lys His Ser Tyr Phe Gly Val Thr His Phe Val Arg 
    130                 135                 140                 


Pro Ala Tyr Lys Ala Glu Pro Lys Pro Thr Pro Pro Leu Glu Ser Thr 
145                 150                 155                 160 


Pro Ala Thr Arg Pro Val Thr Gly Ser Trp Lys Lys Asn Gln Tyr Gly 
                165                 170                 175     


Thr Trp Tyr Lys Pro Glu Asn Ala Thr Phe Val Asn Gly Asn Gln Pro 
            180                 185                 190         


Ile Val Thr Arg Ile Gly Ser Pro Phe Leu Asn Ala Pro Val Gly Gly 
        195                 200                 205             


Asn Leu Pro Ala Gly Ala Thr Ile Val Tyr Asp Glu Val Cys Ile Gln 
    210                 215                 220                 


Ala Gly His Ile Trp Ile Gly Tyr Asn Ala Tyr Asn Gly Asn Arg Val 
225                 230                 235                 240 


Tyr Cys Pro Val Arg Thr Cys Gln Gly Val Pro Pro Asn Gln Ile Pro 
                245                 250                 255     


Gly Val Ala Trp Gly Val Phe Lys Val Glu 
            260                 265     


<210>  8
<211>  273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized Fusion Protein

<400>  8

Met Ala Leu Pro Lys Thr Gly Lys Pro Thr Ala Lys Gln Val Val Asp 
1               5                   10                  15      


Trp Ala Ile Asn Leu Ile Gly Ser Gly Val Asp Val Asp Gly Tyr Tyr 
            20                  25                  30          


Gly Arg Gln Cys Trp Asp Leu Pro Asn Tyr Ile Phe Asn Arg Tyr Trp 
        35                  40                  45              


Asn Phe Lys Thr Pro Gly Asn Ala Arg Asp Met Ala Trp Tyr Arg Tyr 
    50                  55                  60                  


Pro Glu Gly Phe Lys Val Phe Arg Asn Thr Ser Asp Phe Val Pro Lys 
65                  70                  75                  80  


Pro Gly Asp Ile Ala Val Trp Thr Gly Gly Asn Tyr Asn Trp Asn Thr 
                85                  90                  95      


Trp Gly His Thr Gly Ile Val Val Gly Pro Ser Thr Lys Ser Tyr Phe 
            100                 105                 110         


Tyr Ser Val Asp Gln Asn Trp Asn Asn Ser Asn Ser Tyr Val Gly Ser 
        115                 120                 125             


Pro Ala Ala Lys Ile Lys His Ser Tyr Phe Gly Val Thr His Phe Val 
    130                 135                 140                 


Arg Pro Ala Tyr Lys Ala Glu Pro Lys Pro Thr Pro Pro Leu Glu Ser 
145                 150                 155                 160 


Thr Pro Ala Thr Arg Pro Val Thr Gly Ser Trp Lys Lys Asn Gln Tyr 
                165                 170                 175     


Gly Thr Trp Tyr Lys Pro Glu Asn Ala Thr Phe Val Asn Gly Asn Gln 
            180                 185                 190         


Pro Ile Val Thr Arg Ile Gly Ser Pro Phe Leu Asn Ala Pro Val Gly 
        195                 200                 205             


Gly Asn Leu Pro Ala Gly Ala Thr Ile Val Tyr Asp Glu Val Cys Ile 
    210                 215                 220                 


Gln Ala Gly His Ile Trp Ile Gly Tyr Asn Ala Tyr Asn Gly Asn Arg 
225                 230                 235                 240 


Val Tyr Cys Pro Val Arg Thr Cys Gln Gly Val Pro Pro Asn Gln Ile 
                245                 250                 255     


Pro Gly Val Ala Trp Gly Val Phe Lys Val Glu His His His His His 
            260                 265                 270         


His 
    


<210>  9
<211>  272
<212>  PRT
<213>  artificial sequence

<220>
<223>  Chemically Synthesized Fusion Protein

<400>  9

Ala Leu Pro Lys Thr Gly Lys Pro Thr Ala Lys Gln Val Val Asp Trp 
1               5                   10                  15      


Ala Ile Asn Leu Ile Gly Ser Gly Val Asp Val Asp Gly Tyr Tyr Gly 
            20                  25                  30          


Arg Gln Cys Trp Asp Leu Pro Asn Tyr Ile Phe Asn Arg Tyr Trp Asn 
        35                  40                  45              


Phe Lys Thr Pro Gly Asn Ala Arg Asp Met Ala Trp Tyr Arg Tyr Pro 
    50                  55                  60                  


Glu Gly Phe Lys Val Phe Arg Asn Thr Ser Asp Phe Val Pro Lys Pro 
65                  70                  75                  80  


Gly Asp Ile Ala Val Trp Thr Gly Gly Asn Tyr Asn Trp Asn Thr Trp 
                85                  90                  95      


Gly His Thr Gly Ile Val Val Gly Pro Ser Thr Lys Ser Tyr Phe Tyr 
            100                 105                 110         


Ser Val Asp Gln Asn Trp Asn Asn Ser Asn Ser Tyr Val Gly Ser Pro 
        115                 120                 125             


Ala Ala Lys Ile Lys His Ser Tyr Phe Gly Val Thr His Phe Val Arg 
    130                 135                 140                 


Pro Ala Tyr Lys Ala Glu Pro Lys Pro Thr Pro Pro Leu Glu Ser Thr 
145                 150                 155                 160 


Pro Ala Thr Arg Pro Val Thr Gly Ser Trp Lys Lys Asn Gln Tyr Gly 
                165                 170                 175     


Thr Trp Tyr Lys Pro Glu Asn Ala Thr Phe Val Asn Gly Asn Gln Pro 
            180                 185                 190         


Ile Val Thr Arg Ile Gly Ser Pro Phe Leu Asn Ala Pro Val Gly Gly 
        195                 200                 205             


Asn Leu Pro Ala Gly Ala Thr Ile Val Tyr Asp Glu Val Cys Ile Gln 
    210                 215                 220                 


Ala Gly His Ile Trp Ile Gly Tyr Asn Ala Tyr Asn Gly Asn Arg Val 
225                 230                 235                 240 


Tyr Cys Pro Val Arg Thr Cys Gln Gly Val Pro Pro Asn Gln Ile Pro 
                245                 250                 255     


Gly Val Ala Trp Gly Val Phe Lys Val Glu His His His His His His 
            260                 265                 270         


<210>  10
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  6xHis tag

<400>  10

His His His His His His 
1               5       


<210>  11
<211>  471
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Phage 187

<400>  11
atggcactgc ctaaaacggg taaaccaacg gcaaaacagg tggttgactg ggcaatcaat       60

ttaatcggca gtggtgtcga tgttgatggt tattatggtc ggcaatgttg ggatttacct      120

aactatattt ttaatagata ctggaacttt aagacaccag gcaacgcaag agatatggca      180

tggtatagat atcctgaagg gtttaaagtg tttagaaaca cttctgattt tgtccctaaa      240

ccaggtgata tagcagtgtg gacaggtggt aattacaatt ggaacacttg gggacacact      300

ggtattgttg taggtccatc aactaaaagt tacttttata gtgtagatca gaattggaat      360

aactctaact cttacgttgg tagtcctgca gcaaagataa aacatagtta ttttggtgta      420

actcattttg ttagacccgc atacaaagca gaaccgaaac ctacaccacc a               471


<210>  12
<211>  318
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Phage K

<400>  12
agtacaccgg caactagacc agttacaggt tcttggaaaa agaaccagta cggaacttgg       60

tataaaccgg aaaatgcaac atttgtcaat ggtaaccaac ctatagtaac tagaataggt      120

tctccattct taaatgctcc agtaggcggt aacttaccgg caggggctac aattgtatat      180

gacgaagttt gtatccaagc aggtcacatt tggataggtt ataatgctta caacggtaac      240

agagtatatt gccctgttag aacttgtcaa ggtgttccac ctaatcaaat acctggcgtt      300

gcctggggag tattcaaa                                                    318


<210>  13
<211>  822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  13
atggcactgc ctaaaacggg taaaccaacg gcaaaacagg tggttgactg ggcaatcaat       60

ttaatcggca gtggtgtcga tgttgatggt tattatggtc ggcaatgttg ggatttacct      120

aactatattt ttaatagata ctggaacttt aagacaccag gcaacgcaag agatatggca      180

tggtatagat atcctgaagg gtttaaagtg tttagaaaca cttctgattt tgtccctaaa      240

ccaggtgata tagcagtgtg gacaggtggt aattacaatt ggaacacttg gggacacact      300

ggtattgttg taggtccatc aactaaaagt tacttttata gtgtagatca gaattggaat      360

aactctaact cttacgttgg tagtcctgca gcaaagataa aacatagtta ttttggtgta      420

actcattttg ttagacccgc atacaaagca gaaccgaaac ctacaccacc actcgagagt      480

acaccggcaa ctagaccagt tacaggttct tggaaaaaga accagtacgg aacttggtat      540

aaaccggaaa atgcaacatt tgtcaatggt aaccaaccta tagtaactag aataggttct      600

ccattcttaa atgctccagt aggcggtaac ttaccggcag gggctacaat tgtatatgac      660

gaagtttgta tccaagcagg tcacatttgg ataggttata atgcttacaa cggtaacaga      720

gtatattgcc ctgttagaac ttgtcaaggt gttccaccta atcaaatacc tggcgttgcc      780

tggggagtat tcaaagtcga gcaccaccac caccaccact ga                         822


