                         SEQUENCE LISTING

<110>  Isis Innovation Limited
 
<120>  Active site probes

<130>  P38458WO

<150>  GB 1205512.5
<151>  2012-03-28

<160>  32    

<170>  PatentIn version 3.5

<210>  1
<211>  76
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aha Ub


<220>
<221>  SITE
<222>  (1)..(1)
<223>  azidohomoalanine modified residue

<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  azidohomoalanine

<400>  1

Xaa Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75      


<210>  2
<211>  77
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  K6Aha Ub


<220>
<221>  SITE
<222>  (7)..(7)
<223>  azidohomoalanine modified residue

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  azidohomoalanine

<400>  2

Met Ala Gln Ile Phe Val Xaa Thr Leu Thr Gly Lys Thr Ile Thr Leu 
1               5                   10                  15      


Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln 
            20                  25                  30          


Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly 
        35                  40                  45              


Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys 
    50                  55                  60                  


Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75          


<210>  3
<211>  77
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  K11Aha Ub


<220>
<221>  SITE
<222>  (12)..(12)
<223>  azidohomoalanine modified residue

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  azidohomoalanine

<400>  3

Met Ala Gln Ile Phe Val Lys Thr Leu Thr Gly Xaa Thr Ile Thr Leu 
1               5                   10                  15      


Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln 
            20                  25                  30          


Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly 
        35                  40                  45              


Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys 
    50                  55                  60                  


Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75          


<210>  4
<211>  77
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  K27Aha Ub


<220>
<221>  SITE
<222>  (28)..(28)
<223>  azidohomoalanine modified residue

<220>
<221>  MOD_RES
<222>  (28)..(28)
<223>  azidohomoalanine

<400>  4

Met Ala Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu 
1               5                   10                  15      


Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Xaa Ala Lys Ile Gln 
            20                  25                  30          


Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly 
        35                  40                  45              


Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys 
    50                  55                  60                  


Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75          


<210>  5
<211>  77
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  K29Aha Ub


<220>
<221>  SITE
<222>  (30)..(30)
<223>  azidohomoalanine modified residue

<220>
<221>  MOD_RES
<222>  (30)..(30)
<223>  azidohomoalanine

<400>  5

Met Ala Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu 
1               5                   10                  15      


Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Xaa Ile Gln 
            20                  25                  30          


Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly 
        35                  40                  45              


Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys 
    50                  55                  60                  


Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75          


<210>  6
<211>  77
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  K33Aha Ub


<220>
<221>  SITE
<222>  (34)..(34)
<223>  azidohomoalanine modified residue

<220>
<221>  MOD_RES
<222>  (34)..(34)
<223>  azidohomoalanine

<400>  6

Met Ala Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu 
1               5                   10                  15      


Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln 
            20                  25                  30          


Asp Xaa Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly 
        35                  40                  45              


Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys 
    50                  55                  60                  


Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75          


<210>  7
<211>  77
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  K48Aha Ub


<220>
<221>  SITE
<222>  (49)..(49)
<223>  azidohomoalanine modified residue

<220>
<221>  MOD_RES
<222>  (49)..(49)
<223>  azidohomoalanine

<400>  7

Met Ala Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu 
1               5                   10                  15      


Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln 
            20                  25                  30          


Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly 
        35                  40                  45              


Xaa Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys 
    50                  55                  60                  


Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75          


<210>  8
<211>  77
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  K63Aha Ub


<220>
<221>  SITE
<222>  (64)..(64)
<223>  azidohomoalanine modified residue

<220>
<221>  MOD_RES
<222>  (64)..(64)
<223>  azidohomoalanine

<400>  8

Met Ala Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu 
1               5                   10                  15      


Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln 
            20                  25                  30          


Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly 
        35                  40                  45              


Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Xaa 
    50                  55                  60                  


Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75          


<210>  9
<211>  231
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WT Ub

<400>  9
atgcagatct tcgtcaagac gttaaccggt aaaaccataa ctctggaagt tgaaccatcc       60

gataccatcg aaaacgttaa ggctaaaatt caagacaagg aaggaattcc acctgatcaa      120

caaagattga tctttgccgg taagcagctc gaggacggta gaacgctgtc tgattacaac      180

attcagaagg agtcgacctt acatcttgtc ttaagactaa gaggtggttg a               231


<210>  10
<211>  234
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K6M Ub

<400>  10
atggcgcaga tcttcgtcat gacgttaacc ggtaaaacca taactctgga agttgaacca       60

tccgatacca tcgaaaacgt taaggctaaa attcaagaca aggaaggaat tccacctgat      120

caacaaagat tgatctttgc cggtaagcag ctcgaggacg gtagaacgct gtctgattac      180

aacattcaga aggagtcgac cttacatctt gtcttaagac taagaggtgg ttga            234


<210>  11
<211>  234
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K11M Ub

<400>  11
atggcgcaga tcttcgtcaa gacgttaacc ggtatgacca taactctgga agttgaacca       60

tccgatacca tcgaaaacgt taaggctaaa attcaagaca aggaaggaat tccacctgat      120

caacaaagat tgatctttgc cggtaagcag ctcgaggacg gtagaacgct gtctgattac      180

aacattcaga aggagtcgac cttacatctt gtcttaagac taagaggtgg ttga            234


<210>  12
<211>  234
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K27M Ub

<400>  12
atggcgcaga tcttcgtcaa gacgttaacc ggtaaaacca taactctgga agttgaacca       60

tccgatacca tcgaaaacgt tatggctaaa attcaagaca aggaaggaat tccacctgat      120

caacaaagat tgatctttgc cggtaagcag ctcgaggacg gtagaacgct gtctgattac      180

aacattcaga aggagtcgac cttacatctt gtcttaagac taagaggtgg ttga            234


<210>  13
<211>  234
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K29M Ub

<400>  13
atggcgcaga tcttcgtcaa gacgttaacc ggtaaaacca taactctgga agttgaacca       60

tccgatacca tcgaaaacgt taaggctatg attcaagaca aggaaggaat tccacctgat      120

caacaaagat tgatctttgc cggtaagcag ctcgaggacg gtagaacgct gtctgattac      180

aacattcaga aggagtcgac cttacatctt gtcttaagac taagaggtgg ttga            234


<210>  14
<211>  234
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K33M Ub

<400>  14
atggcgcaga tcttcgtcaa gacgttaacc ggtaaaacca taactctgga agttgaacca       60

tccgatacca tcgaaaacgt taaggctaaa attcaagaca tggaaggaat tccacctgat      120

caacaaagat tgatctttgc cggtaagcag ctcgaggacg gtagaacgct gtctgattac      180

aacattcaga aggagtcgac cttacatctt gtcttaagac taagaggtgg ttga            234


<210>  15
<211>  234
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K48M Ub

<400>  15
atggcgcaga tcttcgtcaa gacgttaacc ggtaaaacca taactctgga agttgaacca       60

tccgatacca tcgaaaacgt taaggctaaa attcaagaca aggaaggaat tccacctgat      120

caacaaagat tgatctttgc cggtatgcag ctcgaggacg gtagaacgct gtctgattac      180

aacattcaga aggagtcgac cttacatctt gtcttaagac taagaggtgg ttga            234


<210>  16
<211>  234
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K63M Ub

<400>  16
atggcgcaga tcttcgtcaa gacgttaacc ggtaaaacca taactctgga agttgaacca       60

tccgatacca tcgaaaacgt taaggctaaa attcaagaca aggaaggaat tccacctgat      120

caacaaagat tgatctttgc cggtaagcag ctcgaggacg gtagaacgct gtctgattac      180

aacattcaga tggagtcgac cttacatctt gtcttaagac taagaggtgg ttga            234


<210>  17
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A pos 2 Forward primer

<400>  17
ggagaggatc catggcgcag atcttcgtca ag                                     32


<210>  18
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K6M Forward Primer

<400>  18
ggcgcagatc ttcgtcatga cgttaaccgg taaaac                                 36


<210>  19
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K11M Forward Primer

<400>  19
caagacgtta accggtatga ccataactct ggaag                                  35


<210>  20
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K27M Forward Primer

<400>  20
ccgataccat cgaaaacgtt atggctaaaa ttcaagacaa gg                          42


<210>  21
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K29M Forward Primer

<400>  21
cgaaaacgtt aaggctatga ttcaagacaa ggaag                                  35


<210>  22
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K33M Forward Primer

<400>  22
gctaaaattc aagacatgga aggaattcca cctg                                   34


<210>  23
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K48M Forward Primer

<400>  23
gatctttgcc ggtatgcagc tcgaggac                                          28


<210>  24
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K63M Forward Primer

<400>  24
gattacaaca ttcagatgga gtcgacctta catc                                   34


<210>  25
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A pos 2 Reverse Primer

<400>  25
cttgacgaag atctgcgcca tggatcctct cc                                     32


<210>  26
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K6M Reverse Primer

<400>  26
gttttaccgg ttaacgtcat gacgaagatc tgcgcc                                 36


<210>  27
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K11M Reverse Primer

<400>  27
cttccagagt tatggtcata ccggttaacg tcttg                                  35


<210>  28
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K27M Reverse Primer

<400>  28
ccttgtcttg aattttagcc ataacgtttt cgatggtatc gg                          42


<210>  29
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K29M Reverse Primer

<400>  29
cttccttgtc ttgaatcata gccttaacgt tttcg                                  35


<210>  30
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K33M Reverse Primer

<400>  30
caggtggaat tccttccatg tcttgaattt tagc                                   34


<210>  31
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K48M Reverse Primer

<400>  31
gtcctcgagc tgcataccgg caaagatc                                          28


<210>  32
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  K63M Reverse Primer

<400>  32
gatgtaaggt cgactccatc tgaatgttgt aatc                                   34


