                         SEQUENCE LISTING

<110>  THE UNIVERSITY OF WESTERN ONTARIO
 
<120>  SH2 DOMAIN VARIANTS

<130>  118494.0100

<150>  61/616,167
<151>  2012-03-27

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  111
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  FynSH2wt

<400>  1

Ala Pro Val Asp Ser Ile Gln Ala Glu Glu Trp Tyr Phe Gly Lys Leu 
1               5                   10                  15      


Gly Arg Lys Asp Ala Glu Arg Gln Leu Leu Ser Phe Gly Asn Pro Arg 
            20                  25                  30          


Gly Thr Phe Leu Ile Arg Glu Ser Glu Thr Thr Lys Gly Ala Tyr Ser 
        35                  40                  45              


Leu Ser Ile Arg Asp Trp Asp Asp Met Lys Gly Asp His Val Lys His 
    50                  55                  60                  


Tyr Lys Ile Arg Lys Leu Asp Asn Gly Gly Tyr Tyr Ile Thr Thr Arg 
65                  70                  75                  80  


Ala Gln Phe Glu Thr Leu Gln Gln Leu Val Gln His Tyr Ser Glu Arg 
                85                  90                  95      


Ala Ala Gly Leu Cys Cys Arg Leu Val Val Pro Cys His Lys Gly 
            100                 105                 110     


<210>  2
<211>  333
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: CyslessFynSH2DNA

<400>  2
gctccagttg actctatcca ggcagaagag tggtactttg gaaaacttgg ccgaaaagat       60

gctgagcgac agctattgtc ctttggaaac ccaagaggta cctttcttat ccgcgagagt      120

gaaaccacca aaggtgccta ttcactttct atccgtgatt gggatgatat gaaaggagac      180

catgtcaaac attataaaat tcgcaaactt gacaatggtg gatactacat taccacccgg      240

gcccagtttg aaacacttca gcagcttgta caacattact cagagagagc tgcaggtctc      300

tcctcccgcc tagtagttcc ctctcacaaa ggg                                   333


<210>  3
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: CyslessFynSH2Protein

<400>  3

Ala Pro Val Asp Ser Ile Gln Ala Glu Glu Trp Tyr Phe Gly Lys Leu 
1               5                   10                  15      


Gly Arg Lys Asp Ala Glu Arg Gln Leu Leu Ser Phe Gly Asn Pro Arg 
            20                  25                  30          


Gly Thr Phe Leu Ile Arg Glu Ser Glu Thr Thr Lys Gly Ala Tyr Ser 
        35                  40                  45              


Leu Ser Ile Arg Asp Trp Asp Asp Met Lys Gly Asp His Val Lys His 
    50                  55                  60                  


Tyr Lys Ile Arg Lys Leu Asp Asn Gly Gly Tyr Tyr Ile Thr Thr Arg 
65                  70                  75                  80  


Ala Gln Phe Glu Thr Leu Gln Gln Leu Val Gln His Tyr Ser Glu Arg 
                85                  90                  95      


Ala Ala Gly Leu Ser Ser Arg Leu Val Val Pro Ser His Lys Gly 
            100                 105                 110     


<210>  4
<211>  142
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: HisTagCyslessFynSH2

<400>  4

Met Lys Ile Glu Glu His His His His His His Ser Ser Gly Arg Glu 
1               5                   10                  15      


Asn Leu Tyr Phe Gln Gly Gly Ala Ala Gln Pro Ala Ala Pro Val Asp 
            20                  25                  30          


Ser Ile Gln Ala Glu Glu Trp Tyr Phe Gly Lys Leu Gly Arg Lys Asp 
        35                  40                  45              


Ala Glu Arg Gln Leu Leu Ser Phe Gly Asn Pro Arg Gly Thr Phe Leu 
    50                  55                  60                  


Ile Arg Glu Ser Glu Thr Thr Lys Gly Ala Tyr Ser Leu Ser Ile Arg 
65                  70                  75                  80  


Asp Trp Asp Asp Met Lys Gly Asp His Val Lys His Tyr Lys Ile Arg 
                85                  90                  95      


Lys Leu Asp Asn Gly Gly Tyr Tyr Ile Thr Thr Arg Ala Gln Phe Glu 
            100                 105                 110         


Thr Leu Gln Gln Leu Val Gln His Tyr Ser Glu Arg Ala Ala Gly Leu 
        115                 120                 125             


Ser Ser Arg Leu Val Val Pro Ser His Lys Gly Ala Ala Ala 
    130                 135                 140         


<210>  5
<211>  49
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: T8V

<400>  5

Arg Lys Asp Ala Glu Arg Gln Leu Leu Ser Phe Gly Asn Pro Arg Gly 
1               5                   10                  15      


Thr Phe Leu Ile Arg Glu Ser Glu Thr Val Lys Gly Ala Tyr Ser Leu 
            20                  25                  30          


Ser Ile Arg Asp Trp Asp Asp Met Lys Gly Asp His Val Lys His Tyr 
        35                  40                  45              


Lys 
    


<210>  6
<211>  49
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: S10V

<400>  6

Arg Lys Asp Ala Glu Arg Gln Leu Leu Ser Phe Gly Asn Pro Arg Gly 
1               5                   10                  15      


Thr Phe Leu Ile Arg Glu Ser Glu Thr Thr Lys Gly Ala Tyr Val Leu 
            20                  25                  30          


Ser Ile Arg Asp Trp Asp Asp Met Lys Gly Asp His Val Lys His Tyr 
        35                  40                  45              


Lys 
    


<210>  7
<211>  48
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: deltaT8/S10A/K15L

<400>  7

Arg Lys Asp Ala Glu Arg Gln Leu Leu Ser Phe Gly Asn Pro Arg Gly 
1               5                   10                  15      


Thr Phe Leu Ile Arg Glu Ser Glu Thr Lys Gly Ala Tyr Ala Leu Ser 
            20                  25                  30          


Ile Arg Asp Trp Asp Asp Met Lys Gly Asp His Val Lys His Tyr Leu 
        35                  40                  45              


<210>  8
<211>  49
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: S10A

<400>  8

Arg Lys Asp Ala Glu Arg Gln Leu Leu Ser Phe Gly Asn Pro Arg Gly 
1               5                   10                  15      


Thr Phe Leu Ile Arg Glu Ser Glu Thr Thr Lys Gly Ala Tyr Ala Leu 
            20                  25                  30          


Ser Ile Arg Asp Trp Asp Asp Met Lys Gly Asp His Val Lys His Tyr 
        35                  40                  45              


Lys 
    


<210>  9
<211>  49
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: K15L

<400>  9

Arg Lys Asp Ala Glu Arg Gln Leu Leu Ser Phe Gly Asn Pro Arg Gly 
1               5                   10                  15      


Thr Phe Leu Ile Arg Glu Ser Glu Thr Thr Lys Gly Ala Tyr Ser Leu 
            20                  25                  30          


Ser Ile Arg Asp Trp Asp Asp Met Lys Gly Asp His Val Lys His Tyr 
        35                  40                  45              


Leu 
    


<210>  10
<211>  49
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: S10V/K15L

<400>  10

Arg Lys Asp Ala Glu Arg Gln Leu Leu Ser Phe Gly Asn Pro Arg Gly 
1               5                   10                  15      


Thr Phe Leu Ile Arg Glu Ser Glu Thr Thr Lys Gly Ala Tyr Val Leu 
            20                  25                  30          


Ser Ile Arg Asp Trp Asp Asp Met Lys Gly Asp His Val Lys His Tyr 
        35                  40                  45              


Leu 
    


<210>  11
<211>  49
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: K2E/T8V/S10A/K15I

<400>  11

Arg Glu Asp Ala Glu Arg Gln Leu Leu Ser Phe Gly Asn Pro Arg Gly 
1               5                   10                  15      


Thr Phe Leu Ile Arg Glu Ser Glu Thr Val Lys Gly Ala Tyr Ala Leu 
            20                  25                  30          


Ser Ile Arg Asp Trp Asp Asp Met Lys Gly Asp His Val Lys His Tyr 
        35                  40                  45              


Ile 
    


<210>  12
<211>  49
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: T7S/S10A/K15L

<400>  12

Arg Lys Asp Ala Glu Arg Gln Leu Leu Ser Phe Gly Asn Pro Arg Gly 
1               5                   10                  15      


Thr Phe Leu Ile Arg Glu Ser Glu Ser Thr Lys Gly Ala Tyr Ala Leu 
            20                  25                  30          


Ser Ile Arg Asp Trp Asp Asp Met Lys Gly Asp His Val Lys His Tyr 
        35                  40                  45              


Leu 
    


<210>  13
<211>  49
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: S10A/K15L

<400>  13

Arg Lys Asp Ala Glu Arg Gln Leu Leu Ser Phe Gly Asn Pro Arg Gly 
1               5                   10                  15      


Thr Phe Leu Ile Arg Glu Ser Glu Thr Thr Lys Gly Ala Tyr Ala Leu 
            20                  25                  30          


Ser Ile Arg Asp Trp Asp Asp Met Lys Gly Asp His Val Lys His Tyr 
        35                  40                  45              


Leu 
    


<210>  14
<211>  49
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: T8V/S10A/K15I

<400>  14

Arg Lys Asp Ala Glu Arg Gln Leu Leu Ser Phe Gly Asn Pro Arg Gly 
1               5                   10                  15      


Thr Phe Leu Ile Arg Glu Ser Glu Thr Val Lys Gly Ala Tyr Ala Leu 
            20                  25                  30          


Ser Ile Arg Asp Trp Asp Asp Met Lys Gly Asp His Val Lys His Tyr 
        35                  40                  45              


Ile 
    


<210>  15
<211>  49
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: T8V/K15L

<400>  15

Arg Lys Asp Ala Glu Arg Gln Leu Leu Ser Phe Gly Asn Pro Arg Gly 
1               5                   10                  15      


Thr Phe Leu Ile Arg Glu Ser Glu Thr Val Lys Gly Ala Tyr Ser Leu 
            20                  25                  30          


Ser Ile Arg Asp Trp Asp Asp Met Lys Gly Asp His Val Lys His Tyr 
        35                  40                  45              


Leu 
    


<210>  16
<211>  49
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: T8I/S10A/K15L

<400>  16

Arg Lys Asp Ala Glu Arg Gln Leu Leu Ser Phe Gly Asn Pro Arg Gly 
1               5                   10                  15      


Thr Phe Leu Ile Arg Glu Ser Glu Thr Ile Lys Gly Ala Tyr Ala Leu 
            20                  25                  30          


Ser Ile Arg Asp Trp Asp Asp Met Lys Gly Asp His Val Lys His Tyr 
        35                  40                  45              


Leu 
    


<210>  17
<211>  49
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: T8V/S10A/K15L

<400>  17

Arg Lys Asp Ala Glu Arg Gln Leu Leu Ser Phe Gly Asn Pro Arg Gly 
1               5                   10                  15      


Thr Phe Leu Ile Arg Glu Ser Glu Thr Val Lys Gly Ala Tyr Ala Leu 
            20                  25                  30          


Ser Ile Arg Asp Trp Asp Asp Met Lys Gly Asp His Val Lys His Tyr 
        35                  40                  45              


Leu 
    


<210>  18
<211>  136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: HisTagSrcSH2wt

<400>  18

Met Lys His His His His His His Pro Met Ser Asp Tyr Asp Ile Pro 
1               5                   10                  15      


Thr Thr Glu Asn Leu Tyr Phe Gln Gly Ala Met Asp Ser Ile Gln Ala 
            20                  25                  30          


Glu Glu Trp Tyr Phe Gly Lys Ile Thr Arg Arg Glu Ser Glu Arg Leu 
        35                  40                  45              


Leu Leu Asn Ala Glu Asn Pro Arg Gly Thr Phe Leu Val Arg Glu Ser 
    50                  55                  60                  


Glu Thr Thr Lys Gly Ala Tyr Cys Leu Ser Val Ser Asp Phe Asp Asn 
65                  70                  75                  80  


Ala Lys Gly Leu Asn Val Lys His Tyr Lys Ile Arg Lys Leu Asp Ser 
                85                  90                  95      


Gly Gly Phe Tyr Ile Thr Ser Arg Thr Gln Phe Asn Ser Leu Gln Gln 
            100                 105                 110         


Leu Val Ala Tyr Tyr Ser Lys His Ala Asp Gly Leu Cys His Arg Leu 
        115                 120                 125             


Thr Thr Val Cys Pro Thr Ser Lys 
    130                 135     


<210>  19
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: K15L

<400>  19

Thr Lys Gly Ala Tyr Cys Leu Ser Val Ser Asp Phe Asp Asn Ala Lys 
1               5                   10                  15      


Gly Leu Asn Val Lys His Tyr Leu 
            20                  


<210>  20
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: T8V/C10A

<400>  20

Val Lys Gly Ala Tyr Ala Leu Ser Val Ser Asp Phe Asp Asn Ala Lys 
1               5                   10                  15      


Gly Leu Asn Val Lys His Tyr Lys 
            20                  


<210>  21
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: T8V/C10A/K15L

<400>  21

Val Lys Gly Ala Tyr Ala Leu Ser Val Ser Asp Phe Asp Asn Ala Lys 
1               5                   10                  15      


Gly Leu Asn Val Lys His Tyr Leu 
            20                  


<210>  22
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct: Grb2_triple

<400>  22

Met Lys Pro His Pro Trp Phe Phe Gly Lys Ile Pro Arg Ala Lys Ala 
1               5                   10                  15      


Glu Glu Met Leu Ser Lys Gln Arg His Asp Gly Ala Phe Leu Ile Arg 
            20                  25                  30          


Glu Ser Glu Ser Val Pro Gly Asp Phe Ala Leu Ser Val Lys Phe Gly 
        35                  40                  45              


Asn Asp Val Gln His Phe Leu Val Leu Arg Asp Gly Ala Gly Lys Tyr 
    50                  55                  60                  


Phe Leu Trp Val Val Lys Phe Asn Ser Leu Asn Glu Leu Val Asp Tyr 
65                  70                  75                  80  


His Arg Ser Thr Ser Val Ser Arg Asn Gln Gln Ile Phe Leu Arg Asp 
                85                  90                  95      


Ile Glu Gln Val Pro Gln Gln Pro 
            100                 


<210>  23
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide: fluorescein-GGpYGG peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  PHOSPHORYLATION

<400>  23

Gly Gly Tyr Gly Gly 
1               5   


<210>  24
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide: non-phosphorylated fluorescein-GGYGG peptide

<400>  24

Gly Gly Tyr Gly Gly 
1               5   


<210>  25
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  25

Ser Ser Arg Leu Val Val Pro Ser His Lys Gly 
1               5                   10      


<210>  26
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  26

Ser Ser Arg Leu Val Val Pro Ser His Lys Gly Ala Ala Ala 
1               5                   10                  


<210>  27
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide: FLAG tag sequence

<400>  27

Asp Tyr Lys Asp Asp Asp Asp Lys Cys 
1               5                   

 

1





