                         SEQUENCE LISTING

<110>  THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE 
       SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES
 
<120>  ANTI-MESOTHELIN CHIMERIC ANTIGEN RECEPTORS

<130>  712279

<150>  US 61/614,612
<151>  2012-03-23

<160>  32    

<170>  PatentIn version 3.5

<210>  1
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>   Light chain scFv SS

<400>  1

Asp Ile Glu Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Gly Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Asn Ser Tyr Ser Leu Thr Ile Ser Ser Val Glu Ala Glu 
65                  70                  75                  80  


Asp Asp Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Gly Tyr Pro Leu Thr 
                85                  90                  95      


Phe Gly Ala Gly Thr Lys Leu Glu Ile Lys 
            100                 105     


<210>  2
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light chain scFv SS1

<400>  2

Asp Ile Glu Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Gly Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Asn Ser Tyr Ser Leu Thr Ile Ser Ser Val Glu Ala Glu 
65                  70                  75                  80  


Asp Asp Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Lys His Pro Leu Thr 
                85                  90                  95      


Phe Gly Ala Gly Thr Lys Leu Glu Ile Lys 
            100                 105     


<210>  3
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light chain scFv HN1

<400>  3

Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Ile Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Gly Ile Tyr His Trp 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Lys Ala Ser Ser Leu Ala Ser Gly Ala Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Asp Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Asn Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105             


<210>  4
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain scFv SS

<400>  4

Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Glu Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr 
            20                  25                  30          


Thr Met Asn Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Leu Ile Thr Pro Tyr Asn Gly Ala Ser Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Arg Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Asp Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Gly Tyr Asp Gly Arg Gly Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Val Thr Val Ser Ser 
        115                 


<210>  5
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain scFv SS1

<400>  5

Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Glu Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr 
            20                  25                  30          


Thr Met Asn Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Leu Ile Thr Pro Tyr Asn Gly Ala Ser Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Arg Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Asp Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Gly Tyr Asp Gly Arg Gly Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Val Thr Val Ser Ser 
        115                 


<210>  6
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain scFv HN1

<400>  6

Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Arg Pro Gly 
1               5                   10                  15      


Ala Ser Val Gln Val Ser Cys Arg Ala Ser Gly Tyr Ser Ile Asn Thr 
            20                  25                  30          


Tyr Tyr Met Gln Trp Val Arg Gln Ala Pro Gly Ala Gly Leu Glu Trp 
        35                  40                  45              


Met Gly Val Ile Asn Pro Ser Gly Val Thr Ser Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Leu Thr Asn Asp Thr Ser Thr Asn Thr Val Tyr 
65                  70                  75                  80  


Met Gln Leu Asn Ser Leu Thr Ser Ala Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Trp Ala Leu Trp Gly Asp Phe Gly Met Asp Val Trp Gly Lys 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser 
        115                 120 


<210>  7
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  7

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  8
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  8

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Gly Gly Gly Ser 
1               5                   10                  15  


<210>  9
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GM-CSF leader

<400>  9

Met Val Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln 
            20                  25  


<210>  10
<211>  267
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SS scFv

<400>  10

Met Val Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Gln Val Gln Leu Gln Gln Ser 
            20                  25                  30          


Gly Pro Glu Leu Glu Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys 
        35                  40                  45              


Ala Ser Gly Tyr Ser Phe Thr Gly Tyr Thr Met Asn Trp Val Lys Gln 
    50                  55                  60                  


Ser His Gly Lys Ser Leu Glu Trp Ile Gly Leu Ile Thr Pro Tyr Asn 
65                  70                  75                  80  


Gly Ala Ser Ser Tyr Asn Gln Lys Phe Arg Gly Lys Ala Thr Leu Thr 
                85                  90                  95      


Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Asp Leu Leu Ser Leu Thr 
            100                 105                 110         


Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg Gly Gly Tyr Asp Gly 
        115                 120                 125             


Arg Gly Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 
145                 150                 155                 160 


Ile Glu Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu 
                165                 170                 175     


Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met His 
            180                 185                 190         


Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp 
        195                 200                 205             


Thr Ser Lys Leu Ala Ser Gly Val Pro Gly Arg Phe Ser Gly Ser Gly 
    210                 215                 220                 


Ser Gly Asn Ser Tyr Ser Leu Thr Ile Ser Ser Val Glu Ala Glu Asp 
225                 230                 235                 240 


Asp Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Gly Tyr Pro Leu Thr Phe 
                245                 250                 255     


Gly Ala Gly Thr Lys Leu Glu Ile Lys Ala Ala 
            260                 265         


<210>  11
<211>  265
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SS1 scFv

<400>  11

Met Val Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Gln Val Gln Leu Gln Gln Ser 
            20                  25                  30          


Gly Pro Glu Leu Glu Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys 
        35                  40                  45              


Ala Ser Gly Tyr Ser Phe Thr Gly Tyr Thr Met Asn Trp Val Lys Gln 
    50                  55                  60                  


Ser His Gly Lys Ser Leu Glu Trp Ile Gly Leu Ile Thr Pro Tyr Asn 
65                  70                  75                  80  


Gly Ala Ser Ser Tyr Asn Gln Lys Phe Arg Gly Lys Ala Thr Leu Thr 
                85                  90                  95      


Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Asp Leu Leu Ser Leu Thr 
            100                 105                 110         


Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg Gly Gly Tyr Asp Gly 
        115                 120                 125             


Arg Gly Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Gly Gly Gly Ser Asp 
145                 150                 155                 160 


Ile Glu Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu 
                165                 170                 175     


Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met His 
            180                 185                 190         


Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp 
        195                 200                 205             


Thr Ser Lys Leu Ala Ser Gly Val Pro Gly Arg Phe Ser Gly Ser Gly 
    210                 215                 220                 


Ser Gly Asn Ser Tyr Ser Leu Thr Ile Ser Ser Val Glu Ala Glu Asp 
225                 230                 235                 240 


Asp Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Lys His Pro Leu Thr Phe 
                245                 250                 255     


Gly Ala Gly Thr Lys Leu Glu Ile Lys 
            260                 265 


<210>  12
<211>  268
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HN1 scFv

<400>  12

Met Val Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Ala Gln Val Gln Leu Val Gln 
            20                  25                  30          


Ser Gly Ala Glu Val Lys Arg Pro Gly Ala Ser Val Gln Val Ser Cys 
        35                  40                  45              


Arg Ala Ser Gly Tyr Ser Ile Asn Thr Tyr Tyr Met Gln Trp Val Arg 
    50                  55                  60                  


Gln Ala Pro Gly Ala Gly Leu Glu Trp Met Gly Val Ile Asn Pro Ser 
65                  70                  75                  80  


Gly Val Thr Ser Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Leu Thr 
                85                  90                  95      


Asn Asp Thr Ser Thr Asn Thr Val Tyr Met Gln Leu Asn Ser Leu Thr 
            100                 105                 110         


Ser Ala Asp Thr Ala Val Tyr Tyr Cys Ala Arg Trp Ala Leu Trp Gly 
        115                 120                 125             


Asp Phe Gly Met Asp Val Trp Gly Lys Gly Thr Leu Val Thr Val Ser 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Ile Gly 
                165                 170                 175     


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Gly Ile Tyr His Trp 
            180                 185                 190         


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        195                 200                 205             


Tyr Lys Ala Ser Ser Leu Ala Ser Gly Ala Pro Ser Arg Phe Ser Gly 
    210                 215                 220                 


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
225                 230                 235                 240 


Asp Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Asn Tyr Pro Leu 
                245                 250                 255     


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            260                 265             


<210>  13
<211>  86
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 TM domain

<400>  13

Ala Ala Ala Phe Val Pro Val Phe Leu Pro Ala Lys Pro Thr Thr Thr 
1               5                   10                  15      


Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
            20                  25                  30          


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
        35                  40                  45              


His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro 
    50                  55                  60                  


Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu 
65                  70                  75                  80  


Tyr Cys Asn His Arg Asn 
                85      


<210>  14
<211>  67
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 TM domain

<400>  14

Ala Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser 
1               5                   10                  15      


Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro 
            20                  25                  30          


Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly 
        35                  40                  45              


Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
    50                  55                  60                  


Phe Trp Val 
65          


<210>  15
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 intracellular T cell signaling domain

<400>  15

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  16
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD137 intracellular T cell signaling domain

<400>  16

Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
1               5                   10                  15      


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
            20                  25                  30          


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40                  45          


<210>  17
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3 zeta intracellular T cell signaling domain

<400>  17

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  18
<211>  487
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SSscFv-CD28Z CAR

<400>  18

Met Val Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Gln Val Gln Leu Gln Gln Ser 
            20                  25                  30          


Gly Pro Glu Leu Glu Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys 
        35                  40                  45              


Ala Ser Gly Tyr Ser Phe Thr Gly Tyr Thr Met Asn Trp Val Lys Gln 
    50                  55                  60                  


Ser His Gly Lys Ser Leu Glu Trp Ile Gly Leu Ile Thr Pro Tyr Asn 
65                  70                  75                  80  


Gly Ala Ser Ser Tyr Asn Gln Lys Phe Arg Gly Lys Ala Thr Leu Thr 
                85                  90                  95      


Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Asp Leu Leu Ser Leu Thr 
            100                 105                 110         


Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg Gly Gly Tyr Asp Gly 
        115                 120                 125             


Arg Gly Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 
145                 150                 155                 160 


Ile Glu Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu 
                165                 170                 175     


Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met His 
            180                 185                 190         


Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp 
        195                 200                 205             


Thr Ser Lys Leu Ala Ser Gly Val Pro Gly Arg Phe Ser Gly Ser Gly 
    210                 215                 220                 


Ser Gly Asn Ser Tyr Ser Leu Thr Ile Ser Ser Val Glu Ala Glu Asp 
225                 230                 235                 240 


Asp Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Gly Tyr Pro Leu Thr Phe 
                245                 250                 255     


Gly Ala Gly Thr Lys Leu Glu Ile Lys Ala Ala Ala Ile Glu Val Met 
            260                 265                 270         


Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly Thr Ile Ile 
        275                 280                 285             


His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe Pro Gly Pro 
    290                 295                 300                 


Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys 
305                 310                 315                 320 


Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser 
                325                 330                 335     


Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg 
            340                 345                 350         


Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg 
        355                 360                 365             


Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp 
    370                 375                 380                 


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
385                 390                 395                 400 


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
                405                 410                 415     


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
            420                 425                 430         


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
        435                 440                 445             


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
    450                 455                 460                 


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
465                 470                 475                 480 


Met Gln Ala Leu Pro Pro Arg 
                485         


<210>  19
<211>  551
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SSscFv-CD28BBZ CAR

<400>  19

Met Val Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Gln Val Gln Leu Gln Gln Ser 
            20                  25                  30          


Gly Pro Glu Leu Glu Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys 
        35                  40                  45              


Ala Ser Gly Tyr Ser Phe Thr Gly Tyr Thr Met Asn Trp Val Lys Gln 
    50                  55                  60                  


Ser His Gly Lys Ser Leu Glu Trp Ile Gly Leu Ile Thr Pro Tyr Asn 
65                  70                  75                  80  


Gly Ala Ser Ser Tyr Asn Gln Lys Phe Arg Gly Lys Ala Thr Leu Thr 
                85                  90                  95      


Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Asp Leu Leu Ser Leu Thr 
            100                 105                 110         


Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg Gly Gly Tyr Asp Gly 
        115                 120                 125             


Arg Gly Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 
145                 150                 155                 160 


Ile Glu Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu 
                165                 170                 175     


Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met His 
            180                 185                 190         


Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp 
        195                 200                 205             


Thr Ser Lys Leu Ala Ser Gly Val Pro Gly Arg Phe Ser Gly Ser Gly 
    210                 215                 220                 


Ser Gly Asn Ser Tyr Ser Leu Thr Ile Ser Ser Val Glu Ala Glu Asp 
225                 230                 235                 240 


Asp Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Gly Tyr Pro Leu Thr Phe 
                245                 250                 255     


Gly Ala Gly Thr Lys Leu Glu Ile Lys Ala Ala Ala Phe Val Pro Val 
            260                 265                 270         


Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr 
        275                 280                 285             


Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala 
    290                 295                 300                 


Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe 
305                 310                 315                 320 


Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val 
                325                 330                 335     


Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn Arg 
            340                 345                 350         


Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
        355                 360                 365             


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
    370                 375                 380                 


Arg Asp Phe Ala Ala Tyr Arg Ser Arg Phe Ser Val Val Lys Arg Gly 
385                 390                 395                 400 


Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val 
                405                 410                 415     


Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu 
            420                 425                 430         


Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp 
        435                 440                 445             


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
    450                 455                 460                 


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
465                 470                 475                 480 


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
                485                 490                 495     


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
            500                 505                 510         


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
        515                 520                 525             


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
    530                 535                 540                 


Met Gln Ala Leu Pro Pro Arg 
545                 550     


<210>  20
<211>  487
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SS1scFv-28Z CAR

<400>  20

Met Val Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Gln Val Gln Leu Gln Gln Ser 
            20                  25                  30          


Gly Pro Glu Leu Glu Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys 
        35                  40                  45              


Ala Ser Gly Tyr Ser Phe Thr Gly Tyr Thr Met Asn Trp Val Lys Gln 
    50                  55                  60                  


Ser His Gly Lys Ser Leu Glu Trp Ile Gly Leu Ile Thr Pro Tyr Asn 
65                  70                  75                  80  


Gly Ala Ser Ser Tyr Asn Gln Lys Phe Arg Gly Lys Ala Thr Leu Thr 
                85                  90                  95      


Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Asp Leu Leu Ser Leu Thr 
            100                 105                 110         


Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg Gly Gly Tyr Asp Gly 
        115                 120                 125             


Arg Gly Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Gly Gly Gly Ser Asp 
145                 150                 155                 160 


Ile Glu Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu 
                165                 170                 175     


Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met His 
            180                 185                 190         


Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp 
        195                 200                 205             


Thr Ser Lys Leu Ala Ser Gly Val Pro Gly Arg Phe Ser Gly Ser Gly 
    210                 215                 220                 


Ser Gly Asn Ser Tyr Ser Leu Thr Ile Ser Ser Val Glu Ala Glu Asp 
225                 230                 235                 240 


Asp Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Lys His Pro Leu Thr Phe 
                245                 250                 255     


Gly Ala Gly Thr Lys Leu Glu Ile Lys Ala Ala Ala Ile Glu Val Met 
            260                 265                 270         


Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly Thr Ile Ile 
        275                 280                 285             


His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe Pro Gly Pro 
    290                 295                 300                 


Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys 
305                 310                 315                 320 


Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser 
                325                 330                 335     


Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg 
            340                 345                 350         


Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg 
        355                 360                 365             


Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp 
    370                 375                 380                 


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
385                 390                 395                 400 


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
                405                 410                 415     


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
            420                 425                 430         


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
        435                 440                 445             


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
    450                 455                 460                 


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
465                 470                 475                 480 


Met Gln Ala Leu Pro Pro Arg 
                485         


<210>  21
<211>  551
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SS1scFv-28BBZ CAR

<400>  21

Met Val Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Gln Val Gln Leu Gln Gln Ser 
            20                  25                  30          


Gly Pro Glu Leu Glu Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys 
        35                  40                  45              


Ala Ser Gly Tyr Ser Phe Thr Gly Tyr Thr Met Asn Trp Val Lys Gln 
    50                  55                  60                  


Ser His Gly Lys Ser Leu Glu Trp Ile Gly Leu Ile Thr Pro Tyr Asn 
65                  70                  75                  80  


Gly Ala Ser Ser Tyr Asn Gln Lys Phe Arg Gly Lys Ala Thr Leu Thr 
                85                  90                  95      


Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Asp Leu Leu Ser Leu Thr 
            100                 105                 110         


Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg Gly Gly Tyr Asp Gly 
        115                 120                 125             


Arg Gly Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Gly Gly Gly Ser Asp 
145                 150                 155                 160 


Ile Glu Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu 
                165                 170                 175     


Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met His 
            180                 185                 190         


Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp 
        195                 200                 205             


Thr Ser Lys Leu Ala Ser Gly Val Pro Gly Arg Phe Ser Gly Ser Gly 
    210                 215                 220                 


Ser Gly Asn Ser Tyr Ser Leu Thr Ile Ser Ser Val Glu Ala Glu Asp 
225                 230                 235                 240 


Asp Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Lys His Pro Leu Thr Phe 
                245                 250                 255     


Gly Ala Gly Thr Lys Leu Glu Ile Lys Ala Ala Ala Phe Val Pro Val 
            260                 265                 270         


Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr 
        275                 280                 285             


Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala 
    290                 295                 300                 


Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe 
305                 310                 315                 320 


Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val 
                325                 330                 335     


Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn Arg 
            340                 345                 350         


Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
        355                 360                 365             


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
    370                 375                 380                 


Arg Asp Phe Ala Ala Tyr Arg Ser Arg Phe Ser Val Val Lys Arg Gly 
385                 390                 395                 400 


Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val 
                405                 410                 415     


Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu 
            420                 425                 430         


Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp 
        435                 440                 445             


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
    450                 455                 460                 


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
465                 470                 475                 480 


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
                485                 490                 495     


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
            500                 505                 510         


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
        515                 520                 525             


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
    530                 535                 540                 


Met Gln Ala Leu Pro Pro Arg 
545                 550     


<210>  22
<211>  490
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HN1scFv-28Z

<400>  22

Met Val Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Ala Gln Val Gln Leu Val Gln 
            20                  25                  30          


Ser Gly Ala Glu Val Lys Arg Pro Gly Ala Ser Val Gln Val Ser Cys 
        35                  40                  45              


Arg Ala Ser Gly Tyr Ser Ile Asn Thr Tyr Tyr Met Gln Trp Val Arg 
    50                  55                  60                  


Gln Ala Pro Gly Ala Gly Leu Glu Trp Met Gly Val Ile Asn Pro Ser 
65                  70                  75                  80  


Gly Val Thr Ser Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Leu Thr 
                85                  90                  95      


Asn Asp Thr Ser Thr Asn Thr Val Tyr Met Gln Leu Asn Ser Leu Thr 
            100                 105                 110         


Ser Ala Asp Thr Ala Val Tyr Tyr Cys Ala Arg Trp Ala Leu Trp Gly 
        115                 120                 125             


Asp Phe Gly Met Asp Val Trp Gly Lys Gly Thr Leu Val Thr Val Ser 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Ile Gly 
                165                 170                 175     


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Gly Ile Tyr His Trp 
            180                 185                 190         


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        195                 200                 205             


Tyr Lys Ala Ser Ser Leu Ala Ser Gly Ala Pro Ser Arg Phe Ser Gly 
    210                 215                 220                 


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
225                 230                 235                 240 


Asp Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Asn Tyr Pro Leu 
                245                 250                 255     


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala Ile 
            260                 265                 270         


Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly 
        275                 280                 285             


Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe 
    290                 295                 300                 


Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val 
305                 310                 315                 320 


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
                325                 330                 335     


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
            340                 345                 350         


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
        355                 360                 365             


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg 
    370                 375                 380                 


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
385                 390                 395                 400 


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
                405                 410                 415     


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
            420                 425                 430         


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
        435                 440                 445             


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
    450                 455                 460                 


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
465                 470                 475                 480 


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490 


<210>  23
<211>  554
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HN1scFv-28BBZ CAR

<400>  23

Met Val Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Ala Gln Val Gln Leu Val Gln 
            20                  25                  30          


Ser Gly Ala Glu Val Lys Arg Pro Gly Ala Ser Val Gln Val Ser Cys 
        35                  40                  45              


Arg Ala Ser Gly Tyr Ser Ile Asn Thr Tyr Tyr Met Gln Trp Val Arg 
    50                  55                  60                  


Gln Ala Pro Gly Ala Gly Leu Glu Trp Met Gly Val Ile Asn Pro Ser 
65                  70                  75                  80  


Gly Val Thr Ser Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Leu Thr 
                85                  90                  95      


Asn Asp Thr Ser Thr Asn Thr Val Tyr Met Gln Leu Asn Ser Leu Thr 
            100                 105                 110         


Ser Ala Asp Thr Ala Val Tyr Tyr Cys Ala Arg Trp Ala Leu Trp Gly 
        115                 120                 125             


Asp Phe Gly Met Asp Val Trp Gly Lys Gly Thr Leu Val Thr Val Ser 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Ile Gly 
                165                 170                 175     


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Gly Ile Tyr His Trp 
            180                 185                 190         


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        195                 200                 205             


Tyr Lys Ala Ser Ser Leu Ala Ser Gly Ala Pro Ser Arg Phe Ser Gly 
    210                 215                 220                 


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
225                 230                 235                 240 


Asp Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Asn Tyr Pro Leu 
                245                 250                 255     


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala Phe 
            260                 265                 270         


Val Pro Val Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg 
        275                 280                 285             


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
    290                 295                 300                 


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
305                 310                 315                 320 


Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr 
                325                 330                 335     


Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His 
            340                 345                 350         


Arg Asn Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn 
        355                 360                 365             


Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr 
    370                 375                 380                 


Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Phe Ser Val Val 
385                 390                 395                 400 


Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
                405                 410                 415     


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            420                 425                 430         


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
        435                 440                 445             


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
    450                 455                 460                 


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
465                 470                 475                 480 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
                485                 490                 495     


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
            500                 505                 510         


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
        515                 520                 525             


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
    530                 535                 540                 


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
545                 550                 


<210>  24
<211>  795
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized SS scFv

<400>  24
atggttctgc tggtcaccag cctgctgctg tgcgaactgc cccaccccgc ctttctgctg       60

atccccgaca tccagcaggt ccagctccag cagtctggcc ctgaactgga aaaacctggg      120

gctagcgtga aaatctcctg taaggcatcc ggatactcat tcaccggcta caccatgaat      180

tgggtcaaac agagccacgg caaatcactc gagtggatcg gactcatcac tccctacaat      240

ggcgcctctt catacaatca gaaattccgg ggcaaagcaa cactcactgt ggacaaatcc      300

tcttccaccg cctacatgga tctgctgtct ctcacctctg aggactccgc tgtgtacttt      360

tgtgctcggg ggggatacga cggacggggc ttcgattact gggggcaggg aacaactgtg      420

actgtgtcta gtggaggggg cggatctggg ggaggcggat ccggcggagg aggctccgat      480

attgaactca cccagtctcc cgctatcatg tctgcttcac ctggcgagaa agtgactatg      540

acctgttctg cctcatcttc cgtgtcctac atgcactggt accagcagaa atctggcaca      600

tcccctaaac gctggatcta cgacacctct aaactggcat ctggcgtgcc tggccgcttc      660

tctggctccg gctccggcaa ttcttactct ctcacaatct cctctgtgga ggctgaggat      720

gatgccacat actactgtca gcagtggagt ggctacccac tcacatttgg cgctggcact      780

aaactcgaaa tcaaa                                                       795


<210>  25
<211>  795
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized SS1 scFv

<400>  25
atggttctgc tggtgacatc tctcctgctc tgtgaactgc ctcatcccgc ttttctgctc       60

attcccgaca ttcagcaggt ccagctccag cagtctggcc ctgaactcga aaaacctggc      120

gctagcgtga aaatttcctg taaagcctcc ggctactctt ttactggcta cacaatgaat      180

tgggtgaaac agtctcacgg caaatccctc gaatggatcg gactcatcac accctacaat      240

ggcgcctctt cctacaacca gaaattccgg ggcaaggcaa cactcactgt ggacaaatca      300

tcctctaccg cctacatgga tctgctctcc ctcacatctg aggactccgc tgtctacttt      360

tgtgcccgag gaggatacga cggacgagga ttcgattact ggggacaggg aacaactgtg      420

accgtgtcta gtggcggcgg agggagtgga ggcggaggat cttctggcgg gggatccgat      480

attgaactca cacagtctcc cgctatcatg tctgcttctc ccggcgagaa agtgactatg      540

acttgctctg cttcctcttc tgtgtcctac atgcactggt accagcagaa atctggcaca      600

tcccctaaac ggtggatcta cgatactagc aaactggcat ccggcgtgcc tgggcgattc      660

tctggctctg gctctggcaa ctcttactct ctcacaatct catctgtcga ggctgaggac      720

gatgccacat actactgtca gcagtggtct aaacacccac tcacattcgg cgctggcact      780

aaactggaaa taaaa                                                       795


<210>  26
<211>  804
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized HN1 scFv

<400>  26
atggttctgc tggtgacatc tctcctgctc tgtgaactgc ctcatcccgc ttttctgctc       60

attcccgaca ttcaggctca agtccaactg gtccaaagtg gtgctgaagt caaacgcccg      120

ggtgcctccg tccaagtctc ctgccgtgcc tctggctact cgattaacac ctattacatg      180

cagtgggtcc gtcaagcacc gggtgcaggt ctggaatgga tgggtgtcat caatccgtcc      240

ggcgtgacct catatgcgca gaaatttcaa ggtcgcgtta ccctgacgaa cgataccagc      300

acgaataccg tctacatgca gctgaactct ctgacgagtg cagacaccgc ggtgtattac      360

tgcgcacgtt gggcactgtg gggcgatttc ggcatggatg tttggggcaa aggtacgctg      420

gtgaccgtta gctctggtgg tggtggttct ggtggtggtg gtagtggcgg tggcggttct      480

gatattcaga tgacgcaaag cccgtctacc ctgagtgcct ccattggtga ccgtgttacg      540

atcacctgtc gcgcatccga aggcatctat cattggctgg cttggtacca gcaaaaaccg      600

ggtaaagcgc cgaaactgct gatctataaa gcaagttccc tggcatcggg tgctccgagc      660

cgcttttcag gttcgggtag cggcaccgat ttcacgctga ccatctcatc gctgcagccg      720

gacgatttcg ctacctacta ctgccaacaa tactcaaact acccgctgac cttcggtgga      780

gggaccaagc tggagatcaa acgt                                             804


<210>  27
<211>  1464
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized  SSscFv-CD28Z CAR

<400>  27
atggttctgc tggtcaccag cctgctgctg tgcgaactgc cccaccccgc ctttctgctg       60

atccccgaca tccagcaggt ccagctccag cagtctggcc ctgaactgga aaaacctggg      120

gctagcgtga aaatctcctg taaggcatcc ggatactcat tcaccggcta caccatgaat      180

tgggtcaaac agagccacgg caaatcactc gagtggatcg gactcatcac tccctacaat      240

ggcgcctctt catacaatca gaaattccgg ggcaaagcaa cactcactgt ggacaaatcc      300

tcttccaccg cctacatgga tctgctgtct ctcacctctg aggactccgc tgtgtacttt      360

tgtgctcggg ggggatacga cggacggggc ttcgattact gggggcaggg aacaactgtg      420

actgtgtcta gtggaggggg cggatctggg ggaggcggat ccggcggagg aggctccgat      480

attgaactca cccagtctcc cgctatcatg tctgcttcac ctggcgagaa agtgactatg      540

acctgttctg cctcatcttc cgtgtcctac atgcactggt accagcagaa atctggcaca      600

tcccctaaac gctggatcta cgacacctct aaactggcat ctggcgtgcc tggccgcttc      660

tctggctccg gctccggcaa ttcttactct ctcacaatct cctctgtgga ggctgaggat      720

gatgccacat actactgtca gcagtggagt ggctacccac tcacatttgg cgctggcact      780

aaactcgaaa tcaaagcggc cgcaattgaa gttatgtatc ctcctcctta cctagacaat      840

gagaagagca atggaaccat tatccatgtg aaagggaaac acctttgtcc aagtccccta      900

tttcccggac cttctaagcc cttttgggtg ctggtggtgg ttggtggagt cctggcttgc      960

tatagcttgc tagtaacagt ggcctttatt attttctggg tgaggagtaa gaggagcagg     1020

ctcctgcaca gtgactacat gaacatgact ccccgccgcc ccgggcccac ccgcaagcat     1080

taccagccct atgccccacc acgcgacttc gcagcctatc gctccagagt gaagttcagc     1140

aggagcgcag acgcccccgc gtaccagcag ggccagaacc agctctataa cgagctcaat     1200

ctaggacgaa gagaggagta cgatgttttg gacaagagac gtggccggga ccctgagatg     1260

gggggaaagc cgagaaggaa gaaccctcag gaaggcctgt acaatgaact gcagaaagat     1320

aagatggcgg aggcctacag tgagattggg atgaaaggcg agcgccggag gggcaagggg     1380

cacgatggcc tttaccaggg tctcagtaca gccaccaagg acacctacga cgcccttcac     1440

atgcaggccc tgccccctcg ctaa                                            1464


<210>  28
<211>  1656
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized  SSscFv-CD28BBZ CAR

<400>  28
atggttctgc tggtcaccag cctgctgctg tgcgaactgc cccaccccgc ctttctgctg       60

atccccgaca tccagcaggt ccagctccag cagtctggcc ctgaactgga aaaacctggg      120

gctagcgtga aaatctcctg taaggcatcc ggatactcat tcaccggcta caccatgaat      180

tgggtcaaac agagccacgg caaatcactc gagtggatcg gactcatcac tccctacaat      240

ggcgcctctt catacaatca gaaattccgg ggcaaagcaa cactcactgt ggacaaatcc      300

tcttccaccg cctacatgga tctgctgtct ctcacctctg aggactccgc tgtgtacttt      360

tgtgctcggg ggggatacga cggacggggc ttcgattact gggggcaggg aacaactgtg      420

actgtgtcta gtggaggggg cggatctggg ggaggcggat ccggcggagg aggctccgat      480

attgaactca cccagtctcc cgctatcatg tctgcttcac ctggcgagaa agtgactatg      540

acctgttctg cctcatcttc cgtgtcctac atgcactggt accagcagaa atctggcaca      600

tcccctaaac gctggatcta cgacacctct aaactggcat ctggcgtgcc tggccgcttc      660

tctggctccg gctccggcaa ttcttactct ctcacaatct cctctgtgga ggctgaggat      720

gatgccacat actactgtca gcagtggagt ggctacccac tcacatttgg cgctggcact      780

aaactcgaaa tcaaagcggc cgcattcgtg ccggtcttcc tgccagcgaa gcccaccacg      840

acgccagcgc cgcgaccacc aacaccggcg cccaccatcg cgtcgcagcc cctgtccctg      900

cgcccagagg cgtgccggcc agcggcgggg ggcgcagtgc acacgagggg gctggacttc      960

gcctgtgata tctacatctg ggcgcccttg gccgggactt gtggggtcct tctcctgtca     1020

ctggttatca ccctttactg caaccacagg aacaggagta agaggagcag gctcctgcac     1080

agtgactaca tgaacatgac tccccgccgc cccgggccca cccgcaagca ttaccagccc     1140

tatgccccac cacgcgactt cgcagcctat cgctcccgtt tctctgttgt taaacggggc     1200

agaaagaaac tcctgtatat attcaaacaa ccatttatga gaccagtaca aactactcaa     1260

gaggaagatg gctgtagctg ccgatttcca gaagaagaag aaggaggatg tgaactgaga     1320

gtgaagttca gcaggagcgc agacgccccc gcgtaccagc agggccagaa ccagctctat     1380

aacgagctca atctaggacg aagagaggag tacgatgttt tggacaagag acgtggccgg     1440

gaccctgaga tggggggaaa gccgagaagg aagaaccctc aggaaggcct gtacaatgaa     1500

ctgcagaaag ataagatggc ggaggcctac agtgagattg ggatgaaagg cgagcgccgg     1560

aggggcaagg ggcacgatgg cctttaccag ggtctcagta cagccaccaa ggacacctac     1620

gacgcccttc acatgcaggc cctgccccct cgctaa                               1656


<210>  29
<211>  1464
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized  SS1scFv-28Z CAR

<400>  29
atggttctgc tggtgacatc tctcctgctc tgtgaactgc ctcatcccgc ttttctgctc       60

attcccgaca ttcagcaggt ccagctccag cagtctggcc ctgaactcga aaaacctggc      120

gctagcgtga aaatttcctg taaagcctcc ggctactctt ttactggcta cacaatgaat      180

tgggtgaaac agtctcacgg caaatccctc gaatggatcg gactcatcac accctacaat      240

ggcgcctctt cctacaacca gaaattccgg ggcaaggcaa cactcactgt ggacaaatca      300

tcctctaccg cctacatgga tctgctctcc ctcacatctg aggactccgc tgtctacttt      360

tgtgcccgag gaggatacga cggacgagga ttcgattact ggggacaggg aacaactgtg      420

accgtgtcta gtggcggcgg agggagtgga ggcggaggat cttctggcgg gggatccgat      480

attgaactca cacagtctcc cgctatcatg tctgcttctc ccggcgagaa agtgactatg      540

acttgctctg cttcctcttc tgtgtcctac atgcactggt accagcagaa atctggcaca      600

tcccctaaac ggtggatcta cgatactagc aaactggcat ccggcgtgcc tgggcgattc      660

tctggctctg gctctggcaa ctcttactct ctcacaatct catctgtcga ggctgaggac      720

gatgccacat actactgtca gcagtggtct aaacacccac tcacattcgg cgctggcact      780

aaactggaaa taaaagcggc cgcaattgaa gttatgtatc ctcctcctta cctagacaat      840

gagaagagca atggaaccat tatccatgtg aaagggaaac acctttgtcc aagtccccta      900

tttcccggac cttctaagcc cttttgggtg ctggtggtgg ttggtggagt cctggcttgc      960

tatagcttgc tagtaacagt ggcctttatt attttctggg tgaggagtaa gaggagcagg     1020

ctcctgcaca gtgactacat gaacatgact ccccgccgcc ccgggcccac ccgcaagcat     1080

taccagccct atgccccacc acgcgacttc gcagcctatc gctccagagt gaagttcagc     1140

aggagcgcag acgcccccgc gtaccagcag ggccagaacc agctctataa cgagctcaat     1200

ctaggacgaa gagaggagta cgatgttttg gacaagagac gtggccggga ccctgagatg     1260

gggggaaagc cgagaaggaa gaaccctcag gaaggcctgt acaatgaact gcagaaagat     1320

aagatggcgg aggcctacag tgagattggg atgaaaggcg agcgccggag gggcaagggg     1380

cacgatggcc tttaccaggg tctcagtaca gccaccaagg acacctacga cgcccttcac     1440

atgcaggccc tgccccctcg ctaa                                            1464


<210>  30
<211>  1656
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized  SS1scFv-28BBZ CAR

<400>  30
atggttctgc tggtgacatc tctcctgctc tgtgaactgc ctcatcccgc ttttctgctc       60

attcccgaca ttcagcaggt ccagctccag cagtctggcc ctgaactcga aaaacctggc      120

gctagcgtga aaatttcctg taaagcctcc ggctactctt ttactggcta cacaatgaat      180

tgggtgaaac agtctcacgg caaatccctc gaatggatcg gactcatcac accctacaat      240

ggcgcctctt cctacaacca gaaattccgg ggcaaggcaa cactcactgt ggacaaatca      300

tcctctaccg cctacatgga tctgctctcc ctcacatctg aggactccgc tgtctacttt      360

tgtgcccgag gaggatacga cggacgagga ttcgattact ggggacaggg aacaactgtg      420

accgtgtcta gtggcggcgg agggagtgga ggcggaggat cttctggcgg gggatccgat      480

attgaactca cacagtctcc cgctatcatg tctgcttctc ccggcgagaa agtgactatg      540

acttgctctg cttcctcttc tgtgtcctac atgcactggt accagcagaa atctggcaca      600

tcccctaaac ggtggatcta cgatactagc aaactggcat ccggcgtgcc tgggcgattc      660

tctggctctg gctctggcaa ctcttactct ctcacaatct catctgtcga ggctgaggac      720

gatgccacat actactgtca gcagtggtct aaacacccac tcacattcgg cgctggcact      780

aaactggaaa taaaagcggc cgcattcgtg ccggtcttcc tgccagcgaa gcccaccacg      840

acgccagcgc cgcgaccacc aacaccggcg cccaccatcg cgtcgcagcc cctgtccctg      900

cgcccagagg cgtgccggcc agcggcgggg ggcgcagtgc acacgagggg gctggacttc      960

gcctgtgata tctacatctg ggcgcccttg gccgggactt gtggggtcct tctcctgtca     1020

ctggttatca ccctttactg caaccacagg aacaggagta agaggagcag gctcctgcac     1080

agtgactaca tgaacatgac tccccgccgc cccgggccca cccgcaagca ttaccagccc     1140

tatgccccac cacgcgactt cgcagcctat cgctcccgtt tctctgttgt taaacggggc     1200

agaaagaagc tcctgtatat attcaaacaa ccatttatga gaccagtaca aactactcaa     1260

gaggaagatg gctgtagctg ccgatttcca gaagaagaag aaggaggatg tgaactgaga     1320

gtgaagttca gcaggagcgc agacgccccc gcgtaccagc agggccagaa ccagctctat     1380

aacgagctca atctaggacg aagagaggag tacgatgttt tggacaagag acgtggccgg     1440

gaccctgaga tggggggaaa gccgagaagg aagaaccctc aggaaggcct gtacaatgaa     1500

ctgcagaaag ataagatggc ggaggcctac agtgagattg ggatgaaagg cgagcgccgg     1560

aggggcaagg ggcacgatgg cctttaccag ggtctcagta cagccaccaa ggacacctac     1620

gacgcccttc acatgcaggc cctgccccct cgctaa                               1656


<210>  31
<211>  1473
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized  HN1scFv-28Z

<400>  31
atggttctgc tggtgacatc tctcctgctc tgtgaactgc ctcatcccgc ttttctgctc       60

attcccgaca ttcaggctca agtccaactg gtccaaagtg gtgctgaagt caaacgcccg      120

ggtgcctccg tccaagtctc ctgccgtgcc tctggctact cgattaacac ctattacatg      180

cagtgggtcc gtcaagcacc gggtgcaggt ctggaatgga tgggtgtcat caatccgtcc      240

ggcgtgacct catatgcgca gaaatttcaa ggtcgcgtta ccctgacgaa cgataccagc      300

acgaataccg tctacatgca gctgaactct ctgacgagtg cagacaccgc ggtgtattac      360

tgcgcacgtt gggcactgtg gggcgatttc ggcatggatg tttggggcaa aggtacgctg      420

gtgaccgtta gctctggtgg tggtggttct ggtggtggtg gtagtggcgg tggcggttct      480

gatattcaga tgacgcaaag cccgtctacc ctgagtgcct ccattggtga ccgtgttacg      540

atcacctgtc gcgcatccga aggcatctat cattggctgg cttggtacca gcaaaaaccg      600

ggtaaagcgc cgaaactgct gatctataaa gcaagttccc tggcatcggg tgctccgagc      660

cgcttttcag gttcgggtag cggcaccgat ttcacgctga ccatctcatc gctgcagccg      720

gacgatttcg ctacctacta ctgccaacaa tactcaaact acccgctgac cttcggtgga      780

gggaccaagc tggagatcaa acgtgcggcc gcaattgaag ttatgtatcc tcctccttac      840

ctagacaatg agaagagcaa tggaaccatt atccatgtga aagggaaaca cctttgtcca      900

agtcccctat ttcccggacc ttctaagccc ttttgggtgc tggtggtggt tggtggagtc      960

ctggcttgct atagcttgct agtaacagtg gcctttatta ttttctgggt gaggagtaag     1020

aggagcaggc tcctgcacag tgactacatg aacatgactc cccgccgccc cgggcccacc     1080

cgcaagcatt accagcccta tgccccacca cgcgacttcg cagcctatcg ctccagagtg     1140

aagttcagca ggagcgcaga cgcccccgcg taccagcagg gccagaacca gctctataac     1200

gagctcaatc taggacgaag agaggagtac gatgttttgg acaagagacg tggccgggac     1260

cctgagatgg ggggaaagcc gagaaggaag aaccctcagg aaggcctgta caatgaactg     1320

cagaaagata agatggcgga ggcctacagt gagattggga tgaaaggcga gcgccggagg     1380

ggcaaggggc acgatggcct ttaccagggt ctcagtacag ccaccaagga cacctacgac     1440

gcccttcaca tgcaggccct gccccctcgc taa                                  1473


<210>  32
<211>  1665
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized  HN1scFv-28BBZ CAR

<400>  32
atggttctgc tggtgacatc tctcctgctc tgtgaactgc ctcatcccgc ttttctgctc       60

attcccgaca ttcaggctca agtccaactg gtccaaagtg gtgctgaagt caaacgcccg      120

ggtgcctccg tccaagtctc ctgccgtgcc tctggctact cgattaacac ctattacatg      180

cagtgggtcc gtcaagcacc gggtgcaggt ctggaatgga tgggtgtcat caatccgtcc      240

ggcgtgacct catatgcgca gaaatttcaa ggtcgcgtta ccctgacgaa cgataccagc      300

acgaataccg tctacatgca gctgaactct ctgacgagtg cagacaccgc ggtgtattac      360

tgcgcacgtt gggcactgtg gggcgatttc ggcatggatg tttggggcaa aggtacgctg      420

gtgaccgtta gctctggtgg tggtggttct ggtggtggtg gtagtggcgg tggcggttct      480

gatattcaga tgacgcaaag cccgtctacc ctgagtgcct ccattggtga ccgtgttacg      540

atcacctgtc gcgcatccga aggcatctat cattggctgg cttggtacca gcaaaaaccg      600

ggtaaagcgc cgaaactgct gatctataaa gcaagttccc tggcatcggg tgctccgagc      660

cgcttttcag gttcgggtag cggcaccgat ttcacgctga ccatctcatc gctgcagccg      720

gacgatttcg ctacctacta ctgccaacaa tactcaaact acccgctgac cttcggtgga      780

gggaccaagc tggagatcaa acgtgcggcc gcattcgtgc cggtcttcct gccagcgaag      840

cccaccacga cgccagcgcc gcgaccacca acaccggcgc ccaccatcgc gtcgcagccc      900

ctgtccctgc gcccagaggc gtgccggcca gcggcggggg gcgcagtgca cacgaggggg      960

ctggacttcg cctgtgatat ctacatctgg gcgcccttgg ccgggacttg tggggtcctt     1020

ctcctgtcac tggttatcac cctttactgc aaccacagga acaggagtaa gaggagcagg     1080

ctcctgcaca gtgactacat gaacatgact ccccgccgcc ccgggcccac ccgcaagcat     1140

taccagccct atgccccacc acgcgacttc gcagcctatc gctcccgttt ctctgttgtt     1200

aaacggggca gaaagaagct cctgtatata ttcaaacaac catttatgag accagtacaa     1260

actactcaag aggaagatgg ctgtagctgc cgatttccag aagaagaaga aggaggatgt     1320

gaactgagag tgaagttcag caggagcgca gacgcccccg cgtaccagca gggccagaac     1380

cagctctata acgagctcaa tctaggacga agagaggagt acgatgtttt ggacaagaga     1440

cgtggccggg accctgagat ggggggaaag ccgagaagga agaaccctca ggaaggcctg     1500

tacaatgaac tgcagaaaga taagatggcg gaggcctaca gtgagattgg gatgaaaggc     1560

gagcgccgga ggggcaaggg gcacgatggc ctttaccagg gtctcagtac agccaccaag     1620

gacacctacg acgcccttca catgcaggcc ctgccccctc gctaa                     1665


