                         SEQUENCE LISTING

<110>  Helmholtz Zentrum Mnchen - Deutsches Forschungszentrum fr 
       Gesundheit und Umwelt (GmbH)
 
<120>  A novel method of producing an oocyte carrying a modified target 
       sequence in its genome

<130>  U1181 PCT

<150>  EP 12 16 1060.4
<151>  2012-03-23

<150>  US 61/614,758
<151>  2012-03-23

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  202
<212>  DNA
<213>  Mus musculus

<400>  1
atgcagacac ctcacaagga gcacctgtac aagctgctgg tgatcggcga cctgggtgtg       60

ggcaagacca gcattatcaa gcgctatgtg caccaaaact tctcctcgca ctaccgggcc      120

accattggtg tggacttcgc gctgaaggtg ctccactggg acccagagac ggtggtgcgc      180

ttgcagctct gggacattgc tg                                               202


<210>  2
<211>  6037
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: Plasmid 
       pCAG-RabZFN-Lnew-FokKK"

<400>  2
ggcgcgccgg attcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc       60

attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc      120

tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt      180

aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca      240

cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg      300

taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca      360

gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt      420

cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt      480

attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg      540

gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc      600

gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag      660

cgcgcggcgg gcgggagtcg ctgcgttgcc ttcgccccgt gccccgctcc gcgccgcctc      720

gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg      780

cccttctcct ccgggctgta attagcgctt ggtttaatga cggctcgttt cttttctgtg      840

gctgcgtgaa agccttaaag ggctccggga gggccctttg tgcggggggg agcggctcgg      900

ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggcccgcgc tgcccggcgg      960

ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcgtgtgcg cgaggggagc     1020

gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga ggggaacaaa ggctgcgtgc     1080

ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc ggcggtcggg ctgtaacccc     1140

cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt     1200

gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg     1260

ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggccccgga     1320

gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg     1380

agagggcgca gggacttcct ttgtcccaaa tctggcggag ccgaaatctg ggaggcgccg     1440

ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg ccggcaggaa ggaaatgggc     1500

ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccatctcca gcctcggggc     1560

tgccgcaggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc     1620

gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac     1680

agatccttaa ttaataatac gactcactat aggggccgcc accatgagat ctcctaagaa     1740

aaagaggaag gtggcggccg ctgactacaa ggatgacgac gataaaccat ggggtggcgg     1800

aggtagtcca tttcagtgtc gaatctgtat gagaaacttt tctgacagat ccaatctttc     1860

atctcatatc cgtacacaca ccggggaaaa gccatttgca tgtgacatct gtggccgaaa     1920

gtttgctcgt agccactctc tcttgcggca tactaaaatc cacaccgggg agaagccatt     1980

ccagtgccgc atttgtatgc ggaatttctc caggagtgac tcccttagcg cacacatccg     2040

gacccataca ggagagaagc ccttcgcatg tgacatctgc ggcaggaagt tcgcaacttc     2100

agggtctctg actcggcaca ccaaaataca taccgggcgc cagaagccat tccaatgtag     2160

aatctgtatg cggaatttca gtcaatccgg caatcttgcc cgccacattc gcacacacac     2220

aggagagaag ccttttgcgt gcgacatttg tgggcgcaaa tttgcccagt caggacatct     2280

ttcacgccac actaaaatcc acctgagggg atcccagctc gtcaagtcag aactggaaga     2340

gaagaagtca gaactgcgac ataaactcaa atacgtcccc catgaataca tcgaactgat     2400

cgagattgct aggaactcca cccaggacag aatcctcgag atgaaagtga tggaattctt     2460

tatgaaagtc tacgggtatc ggggcaagca cctgggcgga tctcgcaaac cagatggggc     2520

aatctacact gtgggtagtc ccatcgacta tggcgtgatt gtcgatacca aggcctacag     2580

tgggggttat aatctgccca tcggacaggc tgacgagatg cagcgatacg tgaaggaaaa     2640

ccagacaaga aacaagcata ttaaccccaa tgagtggtgg aaggtgtatc ctagctccgt     2700

cactgaattc aaatttctgt tcgtgtcagg ccactttaag ggaaactaca aagcacagct     2760

gaccaggctc aatcataaga caaactgcaa tggcgccgtg ctcagcgtcg aggaactgct     2820

catcggcgga gaaatgatca aggccgggac cctgacactg gaggaggtgc gacggaaatt     2880

caataacggg gaaatcaact tctaagttta aacgatcacg cgtaaatgat tgcagatcca     2940

ctagttctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg     3000

tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct     3060

aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg     3120

gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg     3180

cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgaga tccactagtt     3240

ctagcctcga ggctagagcg gccgccactg gccgtcgttt tacaacgtcg tgactgggaa     3300

aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt     3360

aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa     3420

tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg     3480

accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc     3540

gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga     3600

tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt     3660

gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat     3720

agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat     3780

ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa     3840

tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa     3900

atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca     3960

tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc     4020

aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc     4080

acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt     4140

acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt     4200

ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg     4260

ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact     4320

caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg     4380

ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga     4440

aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg     4500

aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa     4560

tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac     4620

aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc     4680

cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca     4740

ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga     4800

gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta     4860

agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc     4920

atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc     4980

cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt     5040

cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac     5100

cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct     5160

tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact     5220

tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg     5280

ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata     5340

aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga     5400

cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag     5460

ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg     5520

agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac     5580

ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca     5640

acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg     5700

cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc     5760

gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa     5820

tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt     5880

ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt     5940

aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg     6000

gataacaatt tcacacagga aacagctatg accatga                              6037


<210>  3
<211>  6037
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: Plasmid  
       pCAG-RabZFN-Rnew-FokEL"

<400>  3
ggcgcgccgg attcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc       60

attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc      120

tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt      180

aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca      240

cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg      300

taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca      360

gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt      420

cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt      480

attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg      540

gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc      600

gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag      660

cgcgcggcgg gcgggagtcg ctgcgttgcc ttcgccccgt gccccgctcc gcgccgcctc      720

gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg      780

cccttctcct ccgggctgta attagcgctt ggtttaatga cggctcgttt cttttctgtg      840

gctgcgtgaa agccttaaag ggctccggga gggccctttg tgcggggggg agcggctcgg      900

ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggcccgcgc tgcccggcgg      960

ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcgtgtgcg cgaggggagc     1020

gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga ggggaacaaa ggctgcgtgc     1080

ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc ggcggtcggg ctgtaacccc     1140

cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt     1200

gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg     1260

ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggccccgga     1320

gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg     1380

agagggcgca gggacttcct ttgtcccaaa tctggcggag ccgaaatctg ggaggcgccg     1440

ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg ccggcaggaa ggaaatgggc     1500

ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccatctcca gcctcggggc     1560

tgccgcaggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc     1620

gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac     1680

agatccttaa ttaataatac gactcactat aggggccgcc accatgagat ctcctaagaa     1740

aaagaggaag gtggcggccg ctgactacaa ggatgacgac gataaaccat ggggtggcgg     1800

aggtagtcca ttccagtgta gaatctgtat gcgaaacttt tcaacgtctg ggcacttgtc     1860

caggcacata aggacacaca ccggagagaa acccttcgct tgcgacatat gtggaaggaa     1920

gttcgcccat aaatggcaga ggaacaagca taccaagatt cacacagggg aaaaaccttt     1980

ccagtgtcgg atttgtatgc gaaatttctc agaccgctca gttctacgga gacacattcg     2040

tacccatacc ggcgaaaagc cgtttgcatg cgacatctgt ggcagaaaat ttgctgactc     2100

cagcactcgc aagaagcaca cgaaaattca caccggacgc cagaaaccct ttcagtgtcg     2160

aatttgcatg cgtaactttt ctcgatccga ccatctgtcg gaacacatcc gaacccatac     2220

tggcgaaaag ccattcgcgt gcgacatctg tggtagaaag ttcgccgata agtctaatag     2280

gaagaagcac acgaaaattc acctgcgcgg atcccagctc gtgaaaagcg aactcgaaga     2340

aaagaaaagt gaactgcggc acaaactgaa atacgtccca catgaataca ttgagctgat     2400

cgagattgct aggaactcca cccaggacag aatcctcgag atgaaagtga tggaattctt     2460

tatgaaagtc tacgggtatc ggggcaagca cctgggcgga tctcgcaaac cagatggggc     2520

aatctacact gtgggtagtc ccatcgacta tggcgtgatt gtcgatacca aggcctacag     2580

tgggggttat aatctgccca ttggacaggc tgacgagatg gaacgatacg tggaggaaaa     2640

ccagacaaga aataagcatc tgaaccccaa tgagtggtgg aaagtgtatc ctagctccgt     2700

cactgaattc aagtttctct tcgtgtcagg ccactttaag ggaaactaca aagcacagct     2760

gaccaggctc aatcatatta caaactgcaa tggcgccgtg ctgagcgtcg aggaactgct     2820

catcggcgga gagatgatca aggccggcac actcaccctg gaggaggtcc gccgaaaatt     2880

caataacggg gaaatcaact tctgagttta aacgatcacg cgtaaatgat tgcagatcca     2940

ctagttctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg     3000

tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct     3060

aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg     3120

gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg     3180

cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgaga tccactagtt     3240

ctagcctcga ggctagagcg gccgccactg gccgtcgttt tacaacgtcg tgactgggaa     3300

aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt     3360

aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa     3420

tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg     3480

accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc     3540

gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga     3600

tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt     3660

gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat     3720

agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat     3780

ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa     3840

tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa     3900

atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca     3960

tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc     4020

aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc     4080

acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt     4140

acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt     4200

ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg     4260

ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact     4320

caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg     4380

ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga     4440

aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg     4500

aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa     4560

tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac     4620

aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc     4680

cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca     4740

ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga     4800

gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta     4860

agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc     4920

atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc     4980

cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt     5040

cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac     5100

cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct     5160

tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact     5220

tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg     5280

ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata     5340

aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga     5400

cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag     5460

ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg     5520

agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac     5580

ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca     5640

acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg     5700

cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc     5760

gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa     5820

tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt     5880

ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt     5940

aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg     6000

gataacaatt tcacacagga aacagctatg accatga                              6037


<210>  4
<211>  144
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: Single stranded 
       Oligodesoxynucleotide ODN IDG-WT"

<400>  4
cacctcacaa ggagcacctg tacaagctgc tggtgatcgg cgacctaggt gtgggcaaga       60

ccagcattat caagcgctat gtgcatcaaa atttctcctc gcactatcga gccaccattg      120

gtgtcgactt cgcgctgaag gtgc                                             144


<210>  5
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: Single stranded 
       Oligodesoxynucleotide Primer P_for"

<400>  5
ggcctccagg atgcagacac c                                                 21


<210>  6
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: Single stranded 
       Oligodesoxynucleotide Primer P_rev"

<400>  6
ccagcaatgt cccagagctg c                                                 21


<210>  7
<211>  600
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: Double stranded PCR 
       fragment Rab38 5`-probe"

<400>  7
ctggaaacta aaattcaagg tgttatacat gtgtgaccac ccaggaaaga attcttctgc       60

ttattagatg ctggtagagg ctgaaagtga aggtatgtaa tttcagagga gcagaggaga      120

aagactttgg ccatcacaga ctttttgctc actcttttat acagtttaaa tctcatctct      180

tatatcatct cactacatgg aatcttggaa tcacagtttt caatcttgct ttcttccttc      240

ataattacac agaatacctc acttcataaa gggatacaat gatgaaataa cactgcatgg      300

accgagtgtt atttcttctc tgagcacccc agataaaggt agaatactga tcccaaagac      360

ctggtccttc ttccctcaca gccttaagtt cctcagggaa tactcacctg cccagtgagt      420

ctttctctgc cccctcccct aaataaacac ttaaccacac ataggctttc catattccct      480

ttcctacttt acaggtgcat ttatctagcc gtggctctct gattatttgt ttattcccac      540

aagcacacgg gtatctgcaa agggcagata cctatgaaca aatggttaag tgaatgaata      600


<210>  8
<211>  7858
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: Plasmid 
       pCAG-dRabChtTAL1"

<400>  8
ggcgcgccgg attcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc       60

attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc      120

tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt      180

aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca      240

cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg      300

taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca      360

gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt      420

cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt      480

attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg      540

gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc      600

gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag      660

cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc      720

ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga      780

cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct      840

gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg gggagcggct      900

cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg      960

gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg     1020

ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct     1080

gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc     1140

aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc     1200

tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg     1260

tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc     1320

cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat     1380

cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga     1440

ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg     1500

aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc     1560

ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg     1620

gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct     1680

ttttcctaca gatccttaat taataatacg actcactata ggggccgcca ccatgggacc     1740

taagaaaaag aggaaggtgg cggccgctga ctacaaggat gacgacgata aaccaggtgg     1800

cggaggtagt ggcggaggtg gggtacccgc cagtccagca gcccaggtgg atctgagaac     1860

cctcggctac agccagcagc agcaggagaa gatcaaacca aaagttcgtt ccaccgtcgc     1920

tcagcaccat gaagcactgg tggggcacgg tttcacacac gcccatattg tggctctgtc     1980

tcaacatccc gctgcactcg ggactgtggc cgtcaaatat caggacatga tcgccgctct     2040

gcctgaggca acccacgaag ccattgttgg cgtcggaaag cagtggagcg gtgccagagc     2100

actcgaagca ctcctcaccg tcgccgggga actgcggggt ccaccactcc agtccggact     2160

ggacactgga cagctgctga agatcgctaa acgcggcgga gtgacagctg tggaagctgt     2220

gcacgcttgg aggaatgctc tgacaggagc cccactgaat cttacacccg aacaggtggt     2280

ggccatcgct agtaacattg ggggcaaaca ggctctggaa acagtacagc ggctgttacc     2340

tgtgctgtgc caggctcatg gcctcacacc tcagcaggtc gtcgcaatcg cctccaatgg     2400

cggagggaag caggccctgg aaacggtgca gagactgtta ccagtgctgt gccaggccca     2460

tggcctaaca ccccagcagg tggtggccat cgccagccac gacggcggca agcaggccct     2520

ggaaaccgtg cagaggctgc tgcctgtgct gtgccaggct catggcctga cacctgagca     2580

ggtcgtcgcc atcgccagca acatcggcgg caagcaggcc ctggaaaccg tgcagaggct     2640

gctgccagtg ctgtgccagg cccatggctt aacacccgaa caggtggtgg ccatcgcttc     2700

taatattggg ggcaagcagg ccctggaaac agtccagaga ctgttgcctg tgctgtgcca     2760

ggctcatggc ttgacacctc agcaggtcgt cgctatcgcc tctaataatg ggggcaagca     2820

ggctctggag acagtacagc gcctgttacc agtgctgtgc caggcccacg ggctcacacc     2880

ccagcaggtg gtggcaatcg cttcccatga cggagggaaa caggctctgg aaacggtcca     2940

gaggctgctc cctgtgctgt gccaggctca cggtctaaca ccccagcagg tggtggccat     3000

tgctagcaac aatgggggca agcaggctct ggagacagtg cagcgcctgc tgcctgtgct     3060

gtgccaggct catggcctca cacctcagca ggtcgtcgcc atcgccagcc acgacggcgg     3120

caagcaggcc ctggaaaccg tgcagaggct gctgccagtg ctgtgccagg cccatggcct     3180

aacaccccag caggtggtgg caatcgcctc caatggcgga gggaagcagg ccctggaaac     3240

ggtgcagaga ctgttacctg tgctgtgcca ggctcatggc ctgacacctg agcaggtcgt     3300

cgctatcgct agcaatatcg gagggaagca ggctctggaa actgtccagc gcctgctccc     3360

agtgctgtgc caggcccatg gcttaacacc ccagcaggtg gtggcaattg ctagcaatgg     3420

cggagggaag caggccctgg agactgtcca gagactgcta cctgtgctgt gccaggctca     3480

tggcttgaca cctcagcagg tcgtcgctat cgcctctaat aatgggggca agcaggctct     3540

ggagacagta cagcgcctgt taccagtgct gtgccaggcc cacgggctca caccccagca     3600

ggtggtggcc atcgccagca acggcggcgg caagcaggcc ctggaaaccg tgcagaggct     3660

gctgcctgtg ctgtgccagg ctcacggcct gaccccacag caggtcgtcg ctattgcttc     3720

taatggcgga gggcggcctg ctctggagag cattgtggct cagctgtcca ggcccgatcc     3780

tgccctggct agatccgcac tcactaacga tcatctggtc gctctcgctt gcctcggtgg     3840

acggcccgct ctggacgcag tcaaaaaggg tctcccccat gctcccgcac tgatcaagag     3900

aaccaacagg agaattcctg agggatccga tcgtttaaac cagctcgtga aaagcgaact     3960

cgaagaaaag aaaagtgaac tgcggcacaa actgaaatac gtcccacatg aatacattga     4020

gctgatcgag attgctagga actccaccca ggacagaatc ctcgagatga aagtgatgga     4080

attctttatg aaagtctacg ggtatcgggg caagcacctg ggcggatctc gcaaaccaga     4140

tggggcaatc tacactgtgg gtagtcccat cgactatggc gtgattgtcg ataccaaggc     4200

ctacagtggg ggttataatc tgcccattgg acaggctgac gagatgcagc gatacgtgga     4260

ggaaaaccag acaagaaata agcatatcaa ccccaatgag tggtggaaag tgtatcctag     4320

ctccgtcact gaattcaagt ttctcttcgt gtctggccac tttaagggaa actacaaagc     4380

acagctgacc aggctcaatc atattacaaa ctgcaatggc gccgtgctga gcgtcgagga     4440

actgctcatc ggcggagaga tgatcaaggc cggcacactc accctggagg aggtccgccg     4500

aaaattcaat aacggggaaa tcaacttctg aacgcgtaaa tgattgcaga tccactagtt     4560

ctagaattcc agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaaaaataa     4620

taataaccgg gcagggggga tctgcatgga tctttgtgaa ggaaccttac ttctgtggtg     4680

tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt     4740

taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct     4800

atggaactga tgaatgggag cagtggtgga atgccagatc cagacatgat aagatacatt     4860

gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt     4920

tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac     4980

aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag     5040

taaaacctct acaaatgtgg tatggctgat tatgatctgc ggccgccact ggccgtcgtt     5100

ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat     5160

ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag     5220

ttgcgcagcc tgaatggcga atggaacgcg ccctgtagcg gcgcattaag cgcggcgggt     5280

gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc     5340

gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg     5400

gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat     5460

tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg     5520

ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct     5580

atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa     5640

aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt     5700

taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac     5760

attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa     5820

aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat     5880

tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc     5940

agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga     6000

gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg     6060

cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc     6120

agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag     6180

taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc     6240

tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg     6300

taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg     6360

acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac     6420

ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac     6480

cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg     6540

agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg     6600

tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg     6660

agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac     6720

tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg     6780

ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg     6840

tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc     6900

aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc     6960

tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt     7020

agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc     7080

taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact     7140

caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac     7200

agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag     7260

aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg     7320

gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg     7380

tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga     7440

gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt     7500

ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct     7560

ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg     7620

aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt     7680

aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta     7740

atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta     7800

tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatga       7858


<210>  9
<211>  7858
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: Plasmid 
       pCAG-dRabChtTAL2"

<400>  9
ggcgcgccgg attcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc       60

attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc      120

tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt      180

aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca      240

cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg      300

taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca      360

gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt      420

cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt      480

attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg      540

gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc      600

gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag      660

cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc      720

ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga      780

cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct      840

gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg gggagcggct      900

cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg      960

gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg     1020

ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct     1080

gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc     1140

aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc     1200

tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg     1260

tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc     1320

cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat     1380

cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga     1440

ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg     1500

aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc     1560

ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg     1620

gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct     1680

ttttcctaca gatccttaat taataatacg actcactata ggggccgcca ccatgggacc     1740

taagaaaaag aggaaggtgg cggccgctga ctacaaggat gacgacgata aaccaggtgg     1800

cggaggtagt ggcggaggtg gggtacccgc cagtccagca gcccaggtgg atctgagaac     1860

cctcggctac agccagcagc agcaggagaa gatcaaacca aaagttcgtt ccaccgtcgc     1920

tcagcaccat gaagcactgg tggggcacgg tttcacacac gcccatattg tggctctgtc     1980

tcaacatccc gctgcactcg ggactgtggc cgtcaaatat caggacatga tcgccgctct     2040

gcctgaggca acccacgaag ccattgttgg cgtcggaaag cagtggagcg gtgccagagc     2100

actcgaagca ctcctcaccg tcgccgggga actgcggggt ccaccactcc agtccggact     2160

ggacactgga cagctgctga agatcgctaa acgcggcgga gtgacagctg tggaagctgt     2220

gcacgcttgg aggaatgctc tgacaggagc cccactgaat cttacacccc agcaggtggt     2280

ggccattgct agcaacaatg ggggcaagca ggctctggag acagtgcagc gcctgctgcc     2340

tgtgctgtgc caggctcatg gcctcacacc tcagcaggtc gtcgccattg cttctaacaa     2400

tggagggaag caggctctgg agactgtgca gagactgctg ccagtgctgt gccaggccca     2460

tggcctaaca ccccagcagg tggtggccat cgccagccac gacggcggca agcaggccct     2520

ggaaaccgtg cagaggctgc tgcctgtgct gtgccaggct catggcctga cacctcagca     2580

ggtcgtcgcc atcgccagcc acgacggcgg caagcaggcc ctggaaaccg tgcagaggct     2640

gctgccagtg ctgtgccagg cccatggctt aacaccccag caggtggtgg ccatcgctag     2700

tcatgacggg ggcaaacagg ctctggaaac agtacagcgg ctgttacctg tgctgtgcca     2760

ggctcatggc ttgacacctc agcaggtcgt cgctatcgcc tctaataatg ggggcaagca     2820

ggctctggag acagtacagc gcctgttacc agtgctgtgc caggcccacg ggctcacacc     2880

ccagcaggtg gtggcaattg cttccaataa cggcggaaaa caggctctgg aaaccgtcca     2940

gaggctgctg cctgtgctgt gccaggctca cggtctaaca ccccagcagg tggtggccat     3000

cgcttccaac ggagggggca aacaggctct ggaaacagtg cagaggctgc tgcctgtgct     3060

gtgccaggct catggcctca cacctgagca ggtcgtcgcc atcgccagca acatcggcgg     3120

caagcaggcc ctggaaaccg tgcagaggct gctgccagtg ctgtgccagg cccatggcct     3180

aacaccccag caggtggtgg caattgcttc caataacggc ggaaaacagg ctctggaaac     3240

cgtccagagg ctgctgcctg tgctgtgcca ggctcatggc ctgacacctc agcaggtcgt     3300

cgcaatcgcc tccaatggcg gagggaagca ggccctggaa acggtgcaga gactgttacc     3360

agtgctgtgc caggcccatg gcttaacacc ccagcaggtg gtggcaatcg cctctaataa     3420

cggagggaag caggccctgg aaaccgtgca gagactgtta cctgtgctgt gccaggctca     3480

tggcttgaca cctcagcagg tcgtcgctat cgctagtcat gatggcggaa aacaggctct     3540

ggaaactgtg cagcggctgc tcccagtgct gtgccaggcc cacgggctca caccccagca     3600

ggtggtggca atcgcctcta ataacggagg gaagcaggcc ctggaaaccg tgcagagact     3660

gttacctgtg ctgtgccagg ctcacggcct gaccccacag caggtcgtcg ctattgcttc     3720

taatggcgga gggcggcctg ctctggagag cattgtggct cagctgtcca ggcccgatcc     3780

tgccctggct agatccgcac tcactaacga tcatctggtc gctctcgctt gcctcggtgg     3840

acggcccgct ctggacgcag tcaaaaaggg tctcccccat gctcccgcac tgatcaagag     3900

aaccaacagg agaattcctg agggatccga tcgtttaaac cagctcgtga aaagcgaact     3960

cgaagaaaag aaaagtgaac tgcggcacaa actgaaatac gtcccacatg aatacattga     4020

gctgatcgag attgctagga actccaccca ggacagaatc ctcgagatga aagtgatgga     4080

attctttatg aaagtctacg ggtatcgggg caagcacctg ggcggatctc gcaaaccaga     4140

tggggcaatc tacactgtgg gtagtcccat cgactatggc gtgattgtcg ataccaaggc     4200

ctacagtggg ggttataatc tgcccattgg acaggctgac gagatgcagc gatacgtgga     4260

ggaaaaccag acaagaaata agcatatcaa ccccaatgag tggtggaaag tgtatcctag     4320

ctccgtcact gaattcaagt ttctcttcgt gtctggccac tttaagggaa actacaaagc     4380

acagctgacc aggctcaatc atattacaaa ctgcaatggc gccgtgctga gcgtcgagga     4440

actgctcatc ggcggagaga tgatcaaggc cggcacactc accctggagg aggtccgccg     4500

aaaattcaat aacggggaaa tcaacttctg aacgcgtaaa tgattgcaga tccactagtt     4560

ctagaattcc agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaaaaataa     4620

taataaccgg gcagggggga tctgcatgga tctttgtgaa ggaaccttac ttctgtggtg     4680

tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt     4740

taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct     4800

atggaactga tgaatgggag cagtggtgga atgccagatc cagacatgat aagatacatt     4860

gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt     4920

tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac     4980

aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag     5040

taaaacctct acaaatgtgg tatggctgat tatgatctgc ggccgccact ggccgtcgtt     5100

ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat     5160

ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag     5220

ttgcgcagcc tgaatggcga atggaacgcg ccctgtagcg gcgcattaag cgcggcgggt     5280

gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc     5340

gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg     5400

gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat     5460

tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg     5520

ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct     5580

atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa     5640

aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt     5700

taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac     5760

attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa     5820

aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat     5880

tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc     5940

agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga     6000

gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg     6060

cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc     6120

agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag     6180

taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc     6240

tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg     6300

taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg     6360

acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac     6420

ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac     6480

cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg     6540

agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg     6600

tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg     6660

agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac     6720

tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg     6780

ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg     6840

tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc     6900

aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc     6960

tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt     7020

agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc     7080

taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact     7140

caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac     7200

agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag     7260

aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg     7320

gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg     7380

tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga     7440

gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt     7500

ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct     7560

ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg     7620

aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt     7680

aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta     7740

atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta     7800

tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatga       7858


<210>  10
<211>  932
<212>  PRT
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: RabChtTAL1-Fok"

<400>  10

Met Gly Pro Lys Lys Lys Arg Lys Val Ala Ala Ala Asp Tyr Lys Asp 
1               5                   10                  15      


Asp Asp Asp Lys Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Val Pro 
            20                  25                  30          


Ala Ser Pro Ala Ala Gln Val Asp Leu Arg Thr Leu Gly Tyr Ser Gln 
        35                  40                  45              


Gln Gln Gln Glu Lys Ile Lys Pro Lys Val Arg Ser Thr Val Ala Gln 
    50                  55                  60                  


His His Glu Ala Leu Val Gly His Gly Phe Thr His Ala His Ile Val 
65                  70                  75                  80  


Ala Leu Ser Gln His Pro Ala Ala Leu Gly Thr Val Ala Val Lys Tyr 
                85                  90                  95      


Gln Asp Met Ile Ala Ala Leu Pro Glu Ala Thr His Glu Ala Ile Val 
            100                 105                 110         


Gly Val Gly Lys Gln Trp Ser Gly Ala Arg Ala Leu Glu Ala Leu Leu 
        115                 120                 125             


Thr Val Ala Gly Glu Leu Arg Gly Pro Pro Leu Gln Ser Gly Leu Asp 
    130                 135                 140                 


Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly Gly Val Thr Ala Val 
145                 150                 155                 160 


Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr Gly Ala Pro Leu Asn 
                165                 170                 175     


Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys 
            180                 185                 190         


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala 
        195                 200                 205             


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly 
    210                 215                 220                 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
225                 230                 235                 240 


Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser His 
                245                 250                 255     


Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
            260                 265                 270         


Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala 
        275                 280                 285             


Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
    290                 295                 300                 


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala 
305                 310                 315                 320 


Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
                325                 330                 335     


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val 
            340                 345                 350         


Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val 
        355                 360                 365             


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln 
    370                 375                 380                 


Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu 
385                 390                 395                 400 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
                405                 410                 415     


Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala 
            420                 425                 430         


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly 
        435                 440                 445             


Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys 
    450                 455                 460                 


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala 
465                 470                 475                 480 


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly 
                485                 490                 495     


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
            500                 505                 510         


Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn 
        515                 520                 525             


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
    530                 535                 540                 


Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala 
545                 550                 555                 560 


Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
                565                 570                 575     


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala 
            580                 585                 590         


Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
        595                 600                 605             


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val 
    610                 615                 620                 


Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val 
625                 630                 635                 640 


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln 
                645                 650                 655     


Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Arg Pro Ala Leu Glu 
            660                 665                 670         


Ser Ile Val Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Arg Ser 
        675                 680                 685             


Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg 
    690                 695                 700                 


Pro Ala Leu Asp Ala Val Lys Lys Gly Leu Pro His Ala Pro Ala Leu 
705                 710                 715                 720 


Ile Lys Arg Thr Asn Arg Arg Ile Pro Glu Gly Ser Asp Arg Leu Asn 
                725                 730                 735     


Gln Leu Val Lys Ser Glu Leu Glu Glu Lys Lys Ser Glu Leu Arg His 
            740                 745                 750         


Lys Leu Lys Tyr Val Pro His Glu Tyr Ile Glu Leu Ile Glu Ile Ala 
        755                 760                 765             


Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu Met Lys Val Met Glu Phe 
    770                 775                 780                 


Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys His Leu Gly Gly Ser Arg 
785                 790                 795                 800 


Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly Ser Pro Ile Asp Tyr Gly 
                805                 810                 815     


Val Ile Val Asp Thr Lys Ala Tyr Ser Gly Gly Tyr Asn Leu Pro Ile 
            820                 825                 830         


Gly Gln Ala Asp Glu Met Gln Arg Tyr Val Glu Glu Asn Gln Thr Arg 
        835                 840                 845             


Asn Lys His Ile Asn Pro Asn Glu Trp Trp Lys Val Tyr Pro Ser Ser 
    850                 855                 860                 


Val Thr Glu Phe Lys Phe Leu Phe Val Ser Gly His Phe Lys Gly Asn 
865                 870                 875                 880 


Tyr Lys Ala Gln Leu Thr Arg Leu Asn His Ile Thr Asn Cys Asn Gly 
                885                 890                 895     


Ala Val Leu Ser Val Glu Glu Leu Leu Ile Gly Gly Glu Met Ile Lys 
            900                 905                 910         


Ala Gly Thr Leu Thr Leu Glu Glu Val Arg Arg Lys Phe Asn Asn Gly 
        915                 920                 925             


Glu Ile Asn Phe 
    930         


<210>  11
<211>  932
<212>  PRT
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: RabChtTAL2-Fok"

<400>  11

Met Gly Pro Lys Lys Lys Arg Lys Val Ala Ala Ala Asp Tyr Lys Asp 
1               5                   10                  15      


Asp Asp Asp Lys Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Val Pro 
            20                  25                  30          


Ala Ser Pro Ala Ala Gln Val Asp Leu Arg Thr Leu Gly Tyr Ser Gln 
        35                  40                  45              


Gln Gln Gln Glu Lys Ile Lys Pro Lys Val Arg Ser Thr Val Ala Gln 
    50                  55                  60                  


His His Glu Ala Leu Val Gly His Gly Phe Thr His Ala His Ile Val 
65                  70                  75                  80  


Ala Leu Ser Gln His Pro Ala Ala Leu Gly Thr Val Ala Val Lys Tyr 
                85                  90                  95      


Gln Asp Met Ile Ala Ala Leu Pro Glu Ala Thr His Glu Ala Ile Val 
            100                 105                 110         


Gly Val Gly Lys Gln Trp Ser Gly Ala Arg Ala Leu Glu Ala Leu Leu 
        115                 120                 125             


Thr Val Ala Gly Glu Leu Arg Gly Pro Pro Leu Gln Ser Gly Leu Asp 
    130                 135                 140                 


Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly Gly Val Thr Ala Val 
145                 150                 155                 160 


Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr Gly Ala Pro Leu Asn 
                165                 170                 175     


Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys 
            180                 185                 190         


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala 
        195                 200                 205             


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly 
    210                 215                 220                 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
225                 230                 235                 240 


Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser His 
                245                 250                 255     


Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
            260                 265                 270         


Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala 
        275                 280                 285             


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
    290                 295                 300                 


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala 
305                 310                 315                 320 


Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
                325                 330                 335     


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val 
            340                 345                 350         


Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val 
        355                 360                 365             


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln 
    370                 375                 380                 


Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu 
385                 390                 395                 400 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
                405                 410                 415     


Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala 
            420                 425                 430         


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly 
        435                 440                 445             


Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys 
    450                 455                 460                 


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala 
465                 470                 475                 480 


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly 
                485                 490                 495     


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
            500                 505                 510         


Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn 
        515                 520                 525             


Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
    530                 535                 540                 


Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala 
545                 550                 555                 560 


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
                565                 570                 575     


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala 
            580                 585                 590         


Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
        595                 600                 605             


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val 
    610                 615                 620                 


Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val 
625                 630                 635                 640 


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln 
                645                 650                 655     


Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Arg Pro Ala Leu Glu 
            660                 665                 670         


Ser Ile Val Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Arg Ser 
        675                 680                 685             


Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg 
    690                 695                 700                 


Pro Ala Leu Asp Ala Val Lys Lys Gly Leu Pro His Ala Pro Ala Leu 
705                 710                 715                 720 


Ile Lys Arg Thr Asn Arg Arg Ile Pro Glu Gly Ser Asp Arg Leu Asn 
                725                 730                 735     


Gln Leu Val Lys Ser Glu Leu Glu Glu Lys Lys Ser Glu Leu Arg His 
            740                 745                 750         


Lys Leu Lys Tyr Val Pro His Glu Tyr Ile Glu Leu Ile Glu Ile Ala 
        755                 760                 765             


Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu Met Lys Val Met Glu Phe 
    770                 775                 780                 


Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys His Leu Gly Gly Ser Arg 
785                 790                 795                 800 


Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly Ser Pro Ile Asp Tyr Gly 
                805                 810                 815     


Val Ile Val Asp Thr Lys Ala Tyr Ser Gly Gly Tyr Asn Leu Pro Ile 
            820                 825                 830         


Gly Gln Ala Asp Glu Met Gln Arg Tyr Val Glu Glu Asn Gln Thr Arg 
        835                 840                 845             


Asn Lys His Ile Asn Pro Asn Glu Trp Trp Lys Val Tyr Pro Ser Ser 
    850                 855                 860                 


Val Thr Glu Phe Lys Phe Leu Phe Val Ser Gly His Phe Lys Gly Asn 
865                 870                 875                 880 


Tyr Lys Ala Gln Leu Thr Arg Leu Asn His Ile Thr Asn Cys Asn Gly 
                885                 890                 895     


Ala Val Leu Ser Val Glu Glu Leu Leu Ile Gly Gly Glu Met Ile Lys 
            900                 905                 910         


Ala Gly Thr Leu Thr Leu Glu Glu Val Arg Arg Lys Phe Asn Asn Gly 
        915                 920                 925             


Glu Ile Asn Phe 
    930         


<210>  12
<211>  144
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: Oligonucleotide 
       ODNIDG-Cht"

<400>  12
cacctcacaa ggagcacctg tacaagctgc tggtgatcgg cgacctggta gtgggcaaga       60

ccagcattat taaacggtac gtgcatcaaa atttctcctc tcattatcga gccaccattg      120

gtgtggactt cgcgctgaag gtgc                                             144


<210>  13
<211>  14
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: dRabChtTal1 
       recognition site"

<400>  13
atcaagcgct atgt                                                         14


<210>  14
<211>  14
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: dRabChtTal2 
       recognition site"

<400>  14
ggcccggtag tgcg                                                         14


<210>  15
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer P_Rab1"

<400>  15
ctggaaacta aaattcaagg tgttatac                                          28


<210>  16
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: Primer P_Rab2"

<400>  16
tattcattca cttaaccatt tgttc                                             25


<210>  17
<211>  587
<212>  PRT
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: Clo 051; Clostridium 
       spec. 7_2_43 FAA"

<400>  17

Met Ile Asn Ile Ile Asp Val Asn Asn Lys Thr Ile Arg Thr Phe Gly 
1               5                   10                  15      


Trp Val Gln Asn Pro Ser Asn Phe Glu Ser Leu Lys Lys Val Val Ala 
            20                  25                  30          


Ile Phe Asp Asn Thr Ser Lys Thr Tyr Asn Glu Leu Lys Asp Lys Lys 
        35                  40                  45              


Ile Lys Lys Leu Val Asp Glu Arg Asp Gly Gln Lys Glu Leu Leu Asn 
    50                  55                  60                  


Ala Leu Asn Ala Asn Pro Leu Lys Ile Lys Tyr Cys Asn Leu Val Gly 
65                  70                  75                  80  


Thr Ser Phe Thr Pro Arg Ser Ser Ala Arg Cys Asn Gly Ile Val Gln 
                85                  90                  95      


Ala Thr Val Lys Gly Gln Arg Lys Glu Phe Ile Asp Asp Trp Ser Ser 
            100                 105                 110         


Asp Asn Phe Val Arg Trp Ala His Ala Leu Gly Phe Ile Lys Tyr Asn 
        115                 120                 125             


Tyr Asp Thr Asp Thr Phe Glu Ile Thr Asp Val Gly Arg Lys Tyr Val 
    130                 135                 140                 


Gln Ser Glu Asp Asp Ser Asn Glu Glu Ser Thr Ile Leu Glu Glu Ala 
145                 150                 155                 160 


Met Leu Ser Tyr Pro Pro Val Ala Arg Val Leu Thr Leu Leu Ser Asn 
                165                 170                 175     


Gly Glu His Leu Thr Lys Tyr Glu Ile Gly Lys Lys Leu Gly Phe Val 
            180                 185                 190         


Gly Glu Ala Gly Phe Thr Ser Leu Pro Leu Asn Val Leu Ile Met Thr 
        195                 200                 205             


Leu Ala Thr Thr Asp Glu Pro Lys Glu Lys Asn Lys Ile Lys Thr Asp 
    210                 215                 220                 


Trp Asp Gly Ser Ser Asp Lys Tyr Ala Arg Met Ile Ser Gly Trp Leu 
225                 230                 235                 240 


Val Lys Leu Gly Leu Leu Val Gln Arg Pro Lys Leu Val Thr Val Asp 
                245                 250                 255     


Phe Gly Gly Glu Leu Tyr Ser Glu Thr Ile Gly His Ala Tyr Met Ile 
            260                 265                 270         


Thr Asp Arg Gly Leu Lys Ala Val Arg Arg Leu Leu Gly Ile Asn Lys 
        275                 280                 285             


Val Ala Arg Val Ser Lys Asn Val Phe Trp Glu Met Leu Ala Thr Lys 
    290                 295                 300                 


Gly Ile Asp Lys Asn Tyr Ile Arg Thr Arg Arg Ala Tyr Ile Leu Lys 
305                 310                 315                 320 


Ile Leu Ile Glu Ser Asn Lys Val Leu Thr Leu Glu Asp Ile Lys Gly 
                325                 330                 335     


Lys Leu Lys Leu Ala Ser Ile Asn Glu Ser Ile Asn Thr Ile Lys Asp 
            340                 345                 350         


Asp Ile Asn Gly Leu Ile Asn Thr Gly Ile Asn Ile Lys Ser Glu Thr 
        355                 360                 365             


Thr Gly Tyr Lys Ile Tyr Asp Ser Ile Asn Asp Phe Ile Ile Pro Lys 
    370                 375                 380                 


Thr Gly Asp Thr Glu Gly Ile Lys Ser Asn Ile Ser Leu Leu Lys Asp 
385                 390                 395                 400 


Glu Leu Arg Gly Gln Ile Ser His Ile Ser His Glu Tyr Leu Ser Leu 
                405                 410                 415     


Ile Asp Leu Ala Phe Asp Ser Lys Gln Asn Arg Leu Phe Glu Met Lys 
            420                 425                 430         


Val Leu Glu Leu Leu Val Asn Glu Tyr Gly Phe Lys Gly Arg His Leu 
        435                 440                 445             


Gly Gly Ser Arg Lys Pro Asp Gly Ile Val Tyr Ser Thr Thr Leu Glu 
    450                 455                 460                 


Asp Asn Phe Gly Ile Ile Val Asp Thr Lys Ala Tyr Ser Glu Gly Tyr 
465                 470                 475                 480 


Ser Leu Pro Ile Ser Gln Ala Asp Glu Met Glu Arg Tyr Val Arg Glu 
                485                 490                 495     


Asn Ser Asn Arg Asp Glu Glu Val Asn Pro Asn Lys Trp Trp Glu Asn 
            500                 505                 510         


Phe Ser Glu Glu Val Lys Lys Tyr Tyr Phe Val Phe Ile Ser Gly Ser 
        515                 520                 525             


Phe Lys Gly Lys Phe Glu Glu Gln Leu Arg Arg Leu Ser Met Thr Thr 
    530                 535                 540                 


Gly Val Asn Gly Ser Ala Val Asn Val Val Asn Leu Leu Leu Gly Ala 
545                 550                 555                 560 


Glu Lys Ile Arg Ser Gly Glu Met Thr Ile Glu Glu Leu Glu Arg Ala 
                565                 570                 575     


Met Phe Asn Asn Ser Glu Phe Ile Leu Lys Tyr 
            580                 585         


<210>  18
<211>  5852
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: pCAG-linker-FokEL 
       plasmid DNA"

<400>  18
ggcgcgccgg attcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc       60

attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc      120

tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt      180

aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca      240

cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg      300

taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca      360

gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt      420

cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt      480

attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg      540

gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc      600

gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag      660

cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct cggcgccgcc      720

tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac      780

ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg      840

tggctgcgtg aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc      900

ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc      960

ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga     1020

gcgcggccgg gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt     1080

gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc     1140

cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc     1200

gtgcggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc     1260

cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccg     1320

gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg     1380

cgagagggcg cagggacttc ctttgtccca aatctggcgg agccgaaatc tgggaggcgc     1440

cgccgcaccc cctctagcgg gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg     1500

gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg     1560

gctgccgcag ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg     1620

gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct     1680

acagatcctt aattaataat acgactcact ataggggccg ccaccatgag atctgactac     1740

aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga cgatgacaag     1800

atggccccca agaagaagag gaaggtgggg tccggaggca ttcatggggt gccagccgct     1860

atggcggtac catccggact ggacactgga cagctgctga agatcgctaa acgcggcgga     1920

gtgacagctg tggaagctgt gcacgcttgg aggaatgctc tgacaggagc cccactgaat     1980

cttatgagac gacgtctcac ggcctgaccc cacagcaggt cgtcgctatt gcttctaatg     2040

gcggagggcg gcctgctctg gagagcattg tggctcagct gtccaggccc gatcctgccc     2100

tggctagatc tctgagaggc agtggatccc agctcgtgaa aagcgaactc gaagaaaaga     2160

aaagtgaact gcggcacaaa ctgaaatacg tcccacatga atacattgag ctgatcgaga     2220

ttgctaggaa ctccacccag gacagaatcc tcgagatgaa agtgatggaa ttctttatga     2280

aagtctacgg gtatcggggc aagcacctgg gcggatctcg caaaccagat ggggcaatct     2340

acactgtggg tagtcccatc gactatggcg tgattgtcga taccaaggcc tacagtgggg     2400

gttataatct gcccattgga caggctgacg agatggaacg atacgtggag gaaaaccaga     2460

caagaaataa gcatctgaac cccaatgagt ggtggaaagt gtatcctagc tccgtcactg     2520

aattcaagtt tctcttcgtg tcaggccact ttaagggaaa ctacaaagca cagctgacca     2580

ggctcaatca tattacaaac tgcaatggcg ccgtgctgag cgtcgaggaa ctgctcatcg     2640

gcggagagat gatcaaggcc ggcacactca ccctggagga ggtccgccga aaattcaata     2700

acggggaaat caacttctga gtttaaacga tcacgcgtaa atgattgcag atccactagt     2760

tctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc     2820

ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa     2880

aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg     2940

gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg     3000

ggctctatgg cttctgaggc ggaaagaacc agctggggct cgagatccac tagttctagc     3060

ctcgaggcta gagcggccgc cactggccgt cgttttacaa cgtcgtgact gggaaaaccc     3120

tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag     3180

cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga     3240

cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc     3300

tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac     3360

gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag     3420

tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc     3480

atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg     3540

actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata     3600

agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa     3660

cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg     3720

cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga     3780

caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat     3840

ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca     3900

gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc     3960

gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca     4020

atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg     4080

caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca     4140

gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata     4200

accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag     4260

ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg     4320

gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca     4380

acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta     4440

atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct     4500

ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca     4560

gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag     4620

gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat     4680

tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt     4740

taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa     4800

cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga     4860

gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg     4920

gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc     4980

agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag     5040

aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc     5100

agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg     5160

cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac     5220

accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga     5280

aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt     5340

ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag     5400

cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg     5460

gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta     5520

tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc     5580

agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc     5640

aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc     5700

gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca     5760

ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa     5820

caatttcaca caggaaacag ctatgaccat ga                                   5852


<210>  19
<211>  5850
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: pCAG-linker-FokKK 
       plasmid DNA"

<400>  19
ggcgcgccgg attcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc       60

attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc      120

tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt      180

aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca      240

cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg      300

taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca      360

gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt      420

cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt      480

attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg      540

gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc      600

gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag      660

cgcgcggcgg gcgggagtcg ctgcgttgcc ttcgccccgt gccccgctcc gcgccgcctc      720

gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg      780

cccttctcct ccgggctgta attagcgctt ggtttaatga cggctcgttt cttttctgtg      840

gctgcgtgaa agccttaaag ggctccggga gggccctttg tgcggggggg agcggctcgg      900

ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggcccgcgc tgcccggcgg      960

ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcgtgtgcg cgaggggagc     1020

gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga ggggaacaaa ggctgcgtgc     1080

ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc ggcggtcggg ctgtaacccc     1140

cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt     1200

gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg     1260

ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggccccgga     1320

gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg     1380

agagggcgca gggacttcct ttgtcccaaa tctggcggag ccgaaatctg ggaggcgccg     1440

ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg ccggcaggaa ggaaatgggc     1500

ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccatctcca gcctcggggc     1560

tgccgcaggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc     1620

gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac     1680

agatccttaa ttaataatac gactcactat aggggccgcc accatgagat ctgactacaa     1740

agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaagat     1800

ggcccccaag aagaagagga aggtggggtc cggaggcatt catggggtgc cagccgctat     1860

ggcggtacca tccggactgg acactggaca gctgctgaag atcgctaaac gcggcggagt     1920

gacagctgtg gaagctgtgc acgcttggag gaatgctctg acaggagccc cactgaatct     1980

tatgagacga cgtctcacgg cctgacccca cagcaggtcg tcgctattgc ttctaatggc     2040

ggagggcggc ctgctctgga gagcattgtg gctcagctgt ccaggcccga tcctgccctg     2100

gctagatctc tgagaggcag tggatcccag ctcgtcaagt cagaactgga agagaagaag     2160

tcagaactgc gacataaact caaatacgtc ccccatgaat acatcgaact gatcgagatt     2220

gctaggaact ccacccagga cagaatcctc gagatgaaag tgatggaatt ctttatgaaa     2280

gtctacgggt atcggggcaa gcacctgggc ggatctcgca aaccagatgg ggcaatctac     2340

actgtgggta gtcccatcga ctatggcgtg attgtcgata ccaaggccta cagtgggggt     2400

tataatctgc ccatcggaca ggctgacgag atgcagcgat acgtgaagga aaaccagaca     2460

agaaacaagc atattaaccc caatgagtgg tggaaggtgt atcctagctc cgtcactgaa     2520

ttcaaatttc tgttcgtgtc aggccacttt aagggaaact acaaagcaca gctgaccagg     2580

ctcaatcata agacaaactg caatggcgcc gtgctcagcg tcgaggaact gctcatcggc     2640

ggagaaatga tcaaggccgg gaccctgaca ctggaggagg tgcgacggaa attcaataac     2700

ggggaaatca acttctaagt ttaaacgatc acgcgtaaat gattgcagat ccactagttc     2760

tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc     2820

ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa     2880

tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg     2940

gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg     3000

ctctatggct tctgaggcgg aaagaaccag ctggggctcg agatccacta gttctagcct     3060

cgaggctaga gcggccgcca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg     3120

gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg     3180

aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg     3240

cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta     3300

cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt     3360

tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg     3420

ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat     3480

cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac     3540

tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag     3600

ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg     3660

cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg     3720

cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca     3780

ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt     3840

ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga     3900

aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga     3960

actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat     4020

gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca     4080

agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt     4140

cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac     4200

catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct     4260

aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga     4320

gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac     4380

aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat     4440

agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg     4500

ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc     4560

actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc     4620

aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg     4680

gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta     4740

atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg     4800

tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga     4860

tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt     4920

ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag     4980

agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa     5040

ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag     5100

tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca     5160

gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac     5220

cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa     5280

ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc     5340

agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg     5400

tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc     5460

ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc     5520

ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag     5580

ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa     5640

accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga     5700

ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc     5760

ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca     5820

atttcacaca ggaaacagct atgaccatga                                      5850


<210>  20
<211>  6453
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: pCAG-linker-IX-Fok 
       plasmid DNA"

<400>  20
ggcgcgccgg attcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc       60

attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc      120

tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt      180

aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca      240

cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg      300

taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca      360

gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt      420

cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt      480

attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg      540

gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc      600

gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag      660

cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc      720

ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga      780

cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct      840

gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg gggagcggct      900

cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg      960

gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg     1020

ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct     1080

gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc     1140

aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc     1200

tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg     1260

tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc     1320

cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat     1380

cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga     1440

ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg     1500

aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc     1560

ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg     1620

gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct     1680

ttttcctaca gatccttaat taataatacg actcactata ggggccgcca ccatgggacc     1740

taagaaaaag aggaaggtgg cggccgctga ctacaaggat gacgacgata aaccaggtgg     1800

cggaggtagt ggcggaggtg gggtacccgc cagtccagca gcccaggtgg atctgagaac     1860

cctcggctac agccagcagc agcaggagaa gatcaaacca aaggtgcggt ccaccgtcgc     1920

tcagcaccat gaagcactgg tggggcacgg tttcacacac gcccatattg tggctctgtc     1980

tcagcatccc gctgcactcg ggactgtggc cgtcaaatat caggacatga tcgccgctct     2040

gcctgaggca acccacgaag ccattgtggg cgtcggaaag cagtggagcg gtgccagagc     2100

actcgaagca ctcctcaccg tcgccgggga actgcggggt ccaccactcc agtccggact     2160

ggacactgga cagctgctga agatcgctaa acgcggcgga gtgacagctg tggaagctgt     2220

gcacgcttgg aggaatgctc tgacaggagc cccactgaat cttatgagac gacgtctcac     2280

ggcctgaccc cacagcaggt cgtcgctatt gcttctaatg gcggagggcg gcctgctctg     2340

gagagcattg tggctcagct gtccaggccc gatcctgccc tggctagatc cgcactcact     2400

aacgatcatc tggtcgctct cgcttgcctc ggtggacggc ccgctctgga cgcagtcaaa     2460

aagggtctcc cccatgctcc cgcactgatc aagagaacca acaggagaat tcctgaggga     2520

tccgatcgtt taaaccagct cgtgaaaagc gaactcgaag aaaagaaaag tgaactgcgg     2580

cacaaactga aatacgtccc acatgaatac attgagctga tcgagattgc taggaactcc     2640

acccaggaca gaatcctcga gatgaaagtg atggaattct ttatgaaagt ctacgggtat     2700

cggggcaagc acctgggcgg atctcgcaaa ccagatgggg caatctacac tgtgggtagt     2760

cccatcgact atggcgtgat tgtcgatacc aaggcctaca gtgggggtta taatctgccc     2820

attggacagg ctgacgagat gcagcgatac gtggaggaaa accagacaag aaataagcat     2880

atcaacccca atgagtggtg gaaagtgtat cctagctccg tcactgaatt caagtttctc     2940

ttcgtgtcag gccactttaa gggaaactac aaagcacagc tgaccaggct caatcatatt     3000

acaaactgca atggcgccgt gctgagcgtc gaggaactgc tcatcggcgg agagatgatc     3060

aaggccggca cactcaccct ggaggaggtc cgccgaaaat tcaataacgg ggaaatcaac     3120

ttctgaacgc gtaaatgatt gcagatccac tagttctaga attccagctg agcgccggtc     3180

gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg ggggatctgc     3240

atggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac     3300

agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact     3360

gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg     3420

gtggaatgcc agatccagac atgataagat acattgatga gtttggacaa accacaacta     3480

gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa     3540

ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg     3600

ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg     3660

ctgattatga tctgcggccg ccactggccg tcgttttaca acgtcgtgac tgggaaaacc     3720

ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata     3780

gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga     3840

acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg     3900

ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca     3960

cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta     4020

gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc     4080

catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg     4140

gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat     4200

aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta     4260

acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt     4320

gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag     4380

acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca     4440

tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc     4500

agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat     4560

cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc     4620

aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg     4680

gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc     4740

agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat     4800

aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga     4860

gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc     4920

ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc     4980

aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt     5040

aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc     5100

tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc     5160

agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca     5220

ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca     5280

ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt     5340

ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta     5400

acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg     5460

agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc     5520

ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag     5580

cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa     5640

gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc     5700

cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc     5760

gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta     5820

caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag     5880

aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct     5940

tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga     6000

gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc     6060

ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt     6120

atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg     6180

cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg     6240

caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc     6300

cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc     6360

accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata     6420

acaatttcac acaggaaaca gctatgacca tga                                  6453


<210>  21
<211>  199
<212>  PRT
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: Clo051 nuclease 
       domain"

<400>  21

Glu Gly Ile Lys Ser Asn Ile Ser Leu Leu Lys Asp Glu Leu Arg Gly 
1               5                   10                  15      


Gln Ile Ser His Ile Ser His Glu Tyr Leu Ser Leu Ile Asp Leu Ala 
            20                  25                  30          


Phe Asp Ser Lys Gln Asn Arg Leu Phe Glu Met Lys Val Leu Glu Leu 
        35                  40                  45              


Leu Val Asn Glu Tyr Gly Phe Lys Gly Arg His Leu Gly Gly Ser Arg 
    50                  55                  60                  


Lys Pro Asp Gly Ile Val Tyr Ser Thr Thr Leu Glu Asp Asn Phe Gly 
65                  70                  75                  80  


Ile Ile Val Asp Thr Lys Ala Tyr Ser Glu Gly Tyr Ser Leu Pro Ile 
                85                  90                  95      


Ser Gln Ala Asp Glu Met Glu Arg Tyr Val Arg Glu Asn Ser Asn Arg 
            100                 105                 110         


Asp Glu Glu Val Asn Pro Asn Lys Trp Trp Glu Asn Phe Ser Glu Glu 
        115                 120                 125             


Val Lys Lys Tyr Tyr Phe Val Phe Ile Ser Gly Ser Phe Lys Gly Lys 
    130                 135                 140                 


Phe Glu Glu Gln Leu Arg Arg Leu Ser Met Thr Thr Gly Val Asn Gly 
145                 150                 155                 160 


Ser Ala Val Asn Val Val Asn Leu Leu Leu Gly Ala Glu Lys Ile Arg 
                165                 170                 175     


Ser Gly Glu Met Thr Ile Glu Glu Leu Glu Arg Ala Met Phe Asn Asn 
            180                 185                 190         


Ser Glu Phe Ile Leu Lys Tyr 
        195                 


<210>  22
<211>  196
<212>  PRT
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: FokI nuclease domain"

<400>  22

Gln Leu Val Lys Ser Glu Leu Glu Glu Lys Lys Ser Glu Leu Arg His 
1               5                   10                  15      


Lys Leu Lys Tyr Val Pro His Glu Tyr Ile Glu Leu Ile Glu Ile Ala 
            20                  25                  30          


Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu Met Lys Val Met Glu Phe 
        35                  40                  45              


Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys His Leu Gly Gly Ser Arg 
    50                  55                  60                  


Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly Ser Pro Ile Asp Tyr Gly 
65                  70                  75                  80  


Val Ile Val Asp Thr Lys Ala Tyr Ser Gly Gly Tyr Asn Leu Pro Ile 
                85                  90                  95      


Gly Gln Ala Asp Glu Met Gln Arg Tyr Val Glu Glu Asn Gln Thr Arg 
            100                 105                 110         


Asn Lys His Ile Asn Pro Asn Glu Trp Trp Lys Val Tyr Pro Ser Ser 
        115                 120                 125             


Val Thr Glu Phe Lys Phe Leu Phe Val Ser Gly His Phe Lys Gly Asn 
    130                 135                 140                 


Tyr Lys Ala Gln Leu Thr Arg Leu Asn His Ile Thr Asn Cys Asn Gly 
145                 150                 155                 160 


Ala Val Leu Ser Val Glu Glu Leu Leu Ile Gly Gly Glu Met Ile Lys 
                165                 170                 175     


Ala Gly Thr Leu Thr Leu Glu Glu Val Arg Arg Lys Phe Asn Asn Gly 
            180                 185                 190         


Glu Ile Asn Phe 
        195     


