                         SEQUENCE LISTING

<110>  Novartis AG
       CROWE, Michael
       FOULKES, Michael
       FRANCESE, Giancarlo
       GRIMLER, Dominique
       KUESTERS, Ernst
       LAUMEN, Kurt
       LI, Yunzhong
       LIN, Changxue
       NAZOR, Jovana
       RUCH, Thomas
       SMITH, Derek
       SONG, Shiwei
       TENG, Shangjun

<120>  CHEMICAL PROCESS FOR PREPARING
SPIROINDOLONES AND INTERMEDIATES THEREOF

<130>  PAT055051-WO-PCT
<140>	
<141>

<150>	PCT/CN2012/000359
<151>	2012-03-23

<160>  154   

<170>  PatentIn version 3.5

<210>  1
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized transaminase gene from Vibrio fluvialis

<400>  1
atgaacaaac cgcagagctg ggaagcgcgt gcggaaacct atagcctgta tggctttacc       60

gatatgccga gcctgcatca gcgtggcacc gtggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgaacggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttttgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatgcgg cggaaggcaa accgcagaaa      420

cgtaaaattc tgacccgttg gaacgcgtat catggcgtga ccgcggtgag cgcgagcatg      480

accggcaaac cgtataacag cgtgtttggc ctgccgctgc cgggctttgt gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtggcgcgt      600

ctggcccgtg aactggaaga aaccattcag cgtgaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattccgc cggcgaaagg ctattttcag      720

gcgattctgc cgatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggcc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gcaaaaacct gaccgcgggt ttttttccga tgggcgcggt gattctgggt      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tccgcatggt      960

tttaccgcga gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gcattggctt tatgtgggcg     1140

ctggaagcgg tgaaagataa agcgagcaaa accccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggcctgattt gccgtccgct gggccagagc     1260

gtggttctgt gcccgccgtt tattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  2
<211>  453
<212>  PRT
<213>  Vibrio fluvialis

<400>  2

Met Asn Lys Pro Gln Ser Trp Glu Ala Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val Asn Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Phe Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Arg Trp Asn Ala Tyr His Gly Val Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Val His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Arg Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Met Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 


Phe Thr Ala Ser Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Thr Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  3
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  3
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  4
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  4

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  5
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  5
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  6
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  6

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  7
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  7
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcattc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  8
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  8

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  9
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  9
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccgtc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  10
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  10

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Arg Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  11
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  11
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgtttgatgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  12
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  12

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Asp Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  13
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  13
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcgtgc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  14
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  14

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Val Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  15
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  15
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg cctgatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgtttctga ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  16
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  16

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Leu Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Leu Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  17
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  17
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  18
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  18

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  19
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  19
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtgacca cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg cctgatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgtttctga ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agagtttgcg gaagtggcgt aa                        1362


<210>  20
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  20

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Thr Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Leu Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Leu Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Glu 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  21
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  21
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtaa cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg cgccatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgtttctga ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gcaggcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggccagtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  22
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  22

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Ala Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Leu Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Gln Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Ser Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  23
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  23
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg cctgatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgtttctga ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gcaggcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agagtttgcg gaagtggcgt aa                        1362


<210>  24
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  24

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Leu Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Leu Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Gln Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Glu 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  25
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  25
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  26
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  26

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  27
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  27
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg cgccatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gcaggcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  28
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  28

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Ala Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Gln Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  29
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  29
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg cctgatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgtttgtga ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  30
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  30

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Leu Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Val Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  31
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  31
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgcatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgtttgtga ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gcaggcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  32
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  32

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Val Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Gln Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  33
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  33
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gcaggcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  34
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  34

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Gln Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  35
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  35
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  36
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  36

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  37
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  37
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgcatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgtttctga ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  38
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  38

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Leu Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  39
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  39
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  40
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  40

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  41
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  41
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg cctgatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  42
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  42

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Leu Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  43
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  43
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgtttgtga ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  44
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  44

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Val Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  45
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  45
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtaa cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg cctgatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgtttctga ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gcaggcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  46
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  46

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Leu Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Leu Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Gln Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  47
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  47
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg cctgatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gcaggcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  48
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  48

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Leu Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Gln Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  49
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  49
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg cctgatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgtttgtga ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gcgtgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  50
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  50

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Leu Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Val Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Arg Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  51
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  51
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg cgccatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgtttgtga ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  52
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  52

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Ala Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Val Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  53
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  53
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgcatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgct attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  54
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  54

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Ala Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  55
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  55
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg cctgatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgtttctga ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  56
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  56

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Leu Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Leu Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  57
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  57
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg cgccatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgtttctga ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  58
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  58

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Ala Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Leu Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  59
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  59
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgtttctga ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gcgtgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  60
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  60

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Leu Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Arg Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  61
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  61
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgcatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgtttctga ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  62
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  62

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Leu Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  63
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  63
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg cctgatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccggctc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagcg ccaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgggt     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gtcgaccgat gggccagagc     1260

gtggttctgg tgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  64
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  64

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Leu Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Ala Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ala Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Gly 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Met Gly Gln Ser Val Val Leu Val Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  65
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  65
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtgacca cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg cctgatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggctttc cgtataacag cgtgtttggc ctgccggctc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagcg ccaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagccg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgggt     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  66
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  66

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Thr Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Leu Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Ala Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ala Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Gly 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  67
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  67
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgtttgatgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacac acggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgggt     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  68
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  68

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Asp Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr His Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Gly 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  69
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  69
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgtttgatgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggctttc cgtataacag caagtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagcg ccaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacac acggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagccg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgggt     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  70
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  70

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Asp Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Phe Pro Tyr Asn Ser Lys Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ala Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr His Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Gly 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  71
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  71
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgtttgatgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggctttc cgtataacag cgtgtttggc ctgccggctc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagcg ccaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacac acggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  72
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  72

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Asp Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Ala Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ala Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr His Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  73
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  73
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgtttgatgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagccg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgggt     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  74
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  74

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Asp Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Gly 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  75
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  75
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgtttgatgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggctttc cgtataacag cgtgtttggc ctgccggctc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgggt     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agagtttgcg gaagtggcgt aa                        1362


<210>  76
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  76

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Asp Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Ala Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Gly 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Glu 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  77
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  77
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgtttgatgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggctttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagcg ccaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacac acggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgggt     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  78
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  78

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Asp Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ala Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr His Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Gly 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  79
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  79
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgtttgatgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggctttc cgtataacag cgtgtttggc ctgccggctc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagcg ccaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacac acggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgggt     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgct gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  80
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  80

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Asp Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Phe Pro Tyr Asn Ser Val Phe Gly Leu Pro Ala Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ala Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr His Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Gly 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  81
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  81
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

ctggtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgta taacatggtg      180

gcgggctttg atcataaagg cctgattgcg gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttcc aagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgtttcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga atgaggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct ggttgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gatgtggcgt aa                        1362


<210>  82
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  82

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Leu Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Tyr Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Ala Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Pro Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu Phe Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Asn Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Val Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Asp Val Ala 
    450             


<210>  83
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  83
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

ctggtggatg tgcatggcaa ccgttatctg gatgcgaaca gcggcctgta taacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga ttggggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct ggttgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttatcg cgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  84
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  84

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Leu Val Asp Val His Gly Asn Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Tyr Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Gly Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Val Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Ile Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  85
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  85
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

ctggtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgta taacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga ttggggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct ggttgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg cgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  86
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  86

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Leu Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Tyr Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Gly Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Val Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  87
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  87
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

ctggtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgta taacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttcc aagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgtttcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga ttggggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atccaaacct gtgggtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg cgccgccgta cattctgacg gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  88
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  88

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Leu Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Tyr Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Pro Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu Phe Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Gly Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Pro Asn Leu Trp Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  89
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  89
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

ctggtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttcc aagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgtttcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga ttgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct ggttgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg cgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gatgtggcgt aa                        1362


<210>  90
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  90

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Leu Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Pro Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu Phe Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Val Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Asp Val Ala 
    450             


<210>  91
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  91
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

ctggtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgta taacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttcc aagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgtttcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga atggggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct ggttgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttatcg cgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gatgtggcgt aa                        1362


<210>  92
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  92

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Leu Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Tyr Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Pro Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu Phe Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Asn Gly Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Val Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Ile Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Asp Val Ala 
    450             


<210>  93
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  93
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

ctggtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgta taacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttcc aagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgtttcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga ttggggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct ggttgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttatcg cgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  94
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  94

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Leu Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Tyr Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Pro Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu Phe Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Gly Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Val Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Ile Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  95
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  95
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

ctggtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgta taacatggtg      180

gcgggctttg atcataaagg cctgattgcg gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttcc aagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgtttcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga atgaggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gtgggtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggcagcagc     1260

gtggttctgg cgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  96
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  96

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Leu Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Tyr Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Ala Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Pro Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu Phe Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Asn Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Trp Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Ser Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  97
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  97
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc tcagtggtaa cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga atgaggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctgctgga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atccaaacct gtgggtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg cgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gatgtggcgt aa                        1362


<210>  98
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  98

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Ser Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Asn Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Leu Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Pro Asn Leu Trp Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Asp Val Ala 
    450             


<210>  99
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  99
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc tcagtggtaa cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga ttggggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctgctgga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gttggtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggcagcagc     1260

gtggttctgg cgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  100
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  100

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Ser Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Gly Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Leu Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Leu Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Ser Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  101
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  101
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc tcagtggtaa cccatggcga aggcccgtat      120

ctggtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg cgccatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cagctttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctgctgga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct ggttgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg cgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gatgtggcgt aa                        1362


<210>  102
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  102

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Ser Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Leu Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Ala Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Ser Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Leu Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Val Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Asp Val Ala 
    450             


<210>  103
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  103
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc tcagtggtaa cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cagctttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga ttggggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctgctgga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct ggttgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggcagcagc     1260

gtggttctgg cgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  104
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  104

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Ser Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Ser Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Gly Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Leu Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Val Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Ser Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  105
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  105
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc tcagtggtaa cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga atgaggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gtgggtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  106
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  106

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Ser Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Asn Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Trp Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  107
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  107
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc tcagtggtaa cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  108
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  108

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Ser Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  109
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  109
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc tcagtggtaa cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag caagtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga ttggggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctgctgga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gtgggtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg cgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gatgtggcgt aa                        1362


<210>  110
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  110

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Ser Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Lys Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Gly Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Leu Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Trp Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Asp Val Ala 
    450             


<210>  111
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  111
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc tcagtggtaa cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga atgaggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctgctgga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct ggttgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg cgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  112
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  112

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Ser Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Asn Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Leu Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Val Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  113
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  113
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc tcagtggtaa cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga ttggggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctgctgga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gttggtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg cgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  114
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  114

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Ser Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Gly Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Leu Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Leu Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  115
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  115
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc tcagtggtaa cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaattg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga ttggggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctgctgga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct ggttgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gatgtggcgt aa                        1362


<210>  116
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  116

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Ser Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Gly Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Leu Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Val Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Asp Val Ala 
    450             


<210>  117
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  117
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc tcagtggtaa cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcaggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga ttggggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctgctgga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gtgggtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg cgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gatgtggcgt aa                        1362


<210>  118
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  118

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Ser Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Gly Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Leu Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Trp Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Asp Val Ala 
    450             


<210>  119
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  119
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc tcagtggtaa cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga ttggggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctgctgga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct ggttgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg cgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  120
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  120

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Ser Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Gly Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Leu Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Val Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  121
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  121
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga ttgaggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctgctgga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct gttggtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg cgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  122
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  122

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Leu Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Leu Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  123
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  123
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag caagtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga atggggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctgctgga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct ggttgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg cgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  124
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  124

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Lys Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Asn Gly Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Leu Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Val Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  125
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  125
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc tcagtggtaa cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga ttggggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctgctgga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct ggttgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttatcg cgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  126
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  126

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Ser Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Gly Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Leu Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Val Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Ile Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  127
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  127
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc tcagtggtaa cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga atggggaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctgctgga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggtaacct ggttgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggcagcagc     1260

gtggttctgg cgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  128
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  128

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Ser Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Asn Gly Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Leu Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Val Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Ser Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  129
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  129
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagcg ccaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgggt     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gtcgaccgat gggccagaac     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  130
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  130

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ala Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Gly 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Met Gly Gln Asn Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  131
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  131
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtaa cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccggctc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgggt     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gtcgaccgct gggccagaac     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  132
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  132

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Ala Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Gly 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Asn Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  133
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  133
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtaa cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgcatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgtttctga ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagcg ccaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg tgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  134
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  134

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Leu Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ala Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Val Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  135
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  135
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtaa cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg cctgatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacac acggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgggt     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gtcgaccgat gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  136
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  136

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Leu Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr His Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Gly 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Met Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  137
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  137
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgtttctga ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcacgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagcg ccaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  138
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  138

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Leu Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ala Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  139
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  139
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagcg ccaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagccg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgggt     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  140
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  140

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ala Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Gly 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  141
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  141
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgcatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgtttctga ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagccg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg tgccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  142
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  142

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Leu Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Val Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  143
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  143
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg cctgatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  144
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  144

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Leu Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  145
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  145
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gcaggcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagcg ccaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagccg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgggt     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gtcgaccgct gggccagaac     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  146
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  146

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Gln Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ala Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Gly 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Asn Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  147
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  147
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtgacca cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgtttctga ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  148
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  148

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Thr Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Leu Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  149
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  149
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtaa cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg cctgatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgtttctga ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gcaggcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggcatcc cgtataacag cgtgtttggc ctgccggctc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagccg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgggt     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  150
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  150

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Leu Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Leu Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Gln Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Ile Pro Tyr Asn Ser Val Phe Gly Leu Pro Ala Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Gly 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  151
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  151
atgaacaaac cgcagagctg ggaaacgcgt gcggaaacct atagcctgta tgcctttacc       60

cacatgccga gcctacatca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtt caacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagca gtaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacag gcggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgagc     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  152
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  152

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu His Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Phe Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr Gly Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  153
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  153
atgaacaaac cgcagagctg ggaaacgcgt gcggaaaccg tgagcctgta tgcctttacc       60

cacatgccga gcctacgtca gcgtggcacc atggtggtga cccatggcga aggcccgtat      120

atcgtggatg tgcatggccg tcgttatctg gatgcgaaca gcggcctgtg gaacatggtg      180

gcgggctttg atcataaagg cctgattgat gcggcgaaag cgcagtatga acgttttccg      240

ggctatcatg cgttttacgg ccgtatgagc gatcagaccg tgatgctgtc tgaaaaactg      300

gtggaagtga gcccgtttga tagcggccgt gtgttttata ccaacagcgg cagcgaagcg      360

aacgatacca tggtgaaaat gctgtggttt ctgcatcgtg cggaaggcaa accgcagaaa      420

cgtaaaattc tgaccctgaa gaacgcgtat catggctcca ccgcggtgag cgcgagcatg      480

accggccttc cgtataacag cgtgtttggc ctgccgctgc cgggctttct gcatctgacc      540

tgcccgcatt attggcgtta tggcgaagaa ggcgaaaccg aagaacagtt tgtcgcgcgt      600

ctggcccgtg aactggaaga aaccattcag aaggaaggcg cggataccat tgcgggcttt      660

tttgcggaac cggtgatggg tgcgggcggt gttattaccc cgccaaaagg ctattttcag      720

gcgattctga ccatcctgcg caaatatgat attccggtga tcagcgatga agtgatttgc      780

ggctttggtc gtaccggcaa cacctggggc tgcgtgacct atgattttac cccggatgcg      840

attattagcg ccaaaaacct aaccgcgggt ttttttccag taggagcggt gattttgggg      900

ccggaactga gcaaacgtct ggaaaccgcg attgaagcga tcgaagaatt tgatcatggt      960

tttaccacac acggccatcc ggtgggttgt gcgattgcgc tgaaagcgat tgatgtggtg     1020

atgaacgaag gcctggccga aaacgtgcgt cgtctggccc cgcgttttga agaacgtctg     1080

aaacatattg cggaacgtcc gaacattggc gaatatcgtg gtattggctt tatgtgggcg     1140

ctggaagtgg tgaaagataa agcgagcaaa gccccgtttg atggcaacct gagcgtgggt     1200

gaacgtattg cgaacacctg caccgatctg ggactgattt gccgaccgtt gggccagagc     1260

gtggttctgg caccgccgta cattctgacc gaagcgcaga tggatgaaat gttcgataaa     1320

ctggaaaaag cgctggataa agtgtttgcg gaagtggcgt aa                        1362


<210>  154
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of V. fluvialis transaminase

<400>  154

Met Asn Lys Pro Gln Ser Trp Glu Thr Arg Ala Glu Thr Val Ser Leu 
1               5                   10                  15      


Tyr Ala Phe Thr His Met Pro Ser Leu Arg Gln Arg Gly Thr Met Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val His Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Tyr Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Arg Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Leu Lys Asn Ala Tyr His Gly Ser Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Leu Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Leu His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Lys Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Thr Pro Pro Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Thr Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ala Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Val Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Asp His Gly 
305                 310                 315                 320 


Phe Thr Thr His Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Val Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Ala Pro Phe Asp Gly Asn Leu Ser Val Gly 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Ala Pro Pro Tyr Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


