                         SEQUENCE LISTING

<110>  Omeros Corporation
       University of Washington through its Center for Commercialization
       Cummings, W. Jason    
       Tjoelker, Larry W.
       Wood, Christi L.
       Yabuki, Munehisa
       Allison, Daniel S.
       Leppard, John B.
       Maizels, Nancy   
 
<120>  COMPOSITION AND METHOD FOR DIVERSIFICATION OF TARGET SEQUENCES

<130>  980087.402WO

<140> PCT
<141> 2013-03-13

<150> US 61/611,446
<151> 2012-03-15

<160>  42    

<170>  PatentIn version 3.5

<210>  1
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  1
ggggtctcta tggggtctaa gcgtggcc                                          28


<210>  2
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  2
ggccgattct tttctcatga gatccctcca gaag                                   34


<210>  3
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  3
ttccccacaa ccaggccatg cgcctccttg                                        30


<210>  4
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  4
cctgcagaca cccagaggag ggctcagc                                          28


<210>  5
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  5
tgaatgcttt gttagcccta attagggatt gaattgagag                             40


<210>  6
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  6
ccgtgagacc ccccgttgac c                                                 21


<210>  7
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  7
gcccgaccga agtcatcgtc tcctccggtg                                        30


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  8
tttgccttca aatcacccta                                                   20


<210>  9
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  9
gggtctgcgg gctctatggg g                                                 21


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  10
atcgccgcgg caattttggg g                                                 21


<210>  11
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  11
caggagctcg cggggccgtc actgattgcc g                                      31


<210>  12
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  12
gcgcaagctt ccccagcctg ccgccaagtc caag                                   34


<210>  13
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  13
gggtctgcgg gctctatggg g                                                 21


<210>  14
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  14
atcgccgcgg caattttggg g                                                 21


<210>  15
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  15
tcactgattg ccgttttctc ccctctctcc                                        30


<210>  16
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  16
ggtcaacggg gggtctcacg g                                                 21


<210>  17
<211>  101
<212>  PRT
<213>  Gallus gallus

<400>  17

Ala Val Thr Leu Asp Glu Ser Gly Gly Gly Leu Gln Thr Pro Gly Arg 
1               5                   10                  15      


Ala Leu Ser Leu Val Cys Lys Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Asn Met Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Phe Val 
        35                  40                  45              


Ala Gly Ile Asp Asn Thr Gly Arg Tyr Thr Gly Tyr Gly Ser Ala Val 
    50                  55                  60                  


Lys Gly Arg Ala Thr Ile Ser Arg Asp Asn Gly Gln Ser Thr Val Arg 
65                  70                  75                  80  


Leu Gln Leu Asn Asn Leu Arg Ala Glu Asp Thr Gly Thr Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Ala Ala Gly 
            100     


<210>  18
<211>  101
<212>  PRT
<213>  Gallus gallus

<400>  18

Ala Val Thr Leu Asp Glu Ser Gly Gly Gly Leu Gln Thr Pro Gly Gly 
1               5                   10                  15      


Ala Leu Ser Leu Val Cys Lys Ala Ser Gly Phe Thr Phe Ser Ser Asn 
            20                  25                  30          


Ala Met Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Gly Ile Asp Asp Asp Gly Ser Gly Thr Arg Tyr Ala Pro Ala Val 
    50                  55                  60                  


Lys Gly Arg Ala Thr Ile Ser Arg Asp Asn Gly Gln Ser Thr Leu Arg 
65                  70                  75                  80  


Leu Gln Leu Asn Asn Leu Arg Ala Glu Asp Thr Gly Thr Tyr Tyr Cys 
                85                  90                  95      


Thr Lys Cys Ala Tyr 
            100     


<210>  19
<211>  94
<212>  PRT
<213>  Gallus gallus

<400>  19

Ala Leu Thr Gln Pro Ser Ser Val Ser Ala Asn Pro Gly Gly Thr Val 
1               5                   10                  15      


Lys Ile Thr Cys Ser Gly Asp Ser Ser Tyr Tyr Gly Trp Tyr Gln Gln 
            20                  25                  30          


Lys Ala Pro Gly Ser Ala Pro Val Thr Val Ile Tyr Asp Asn Thr Asn 
        35                  40                  45              


Arg Pro Ser Asn Ile Pro Ser Arg Phe Ser Gly Ser Lys Ser Gly Ser 
    50                  55                  60                  


Thr Ala Thr Leu Thr Ile Thr Gly Val Arg Ala Asp Asp Asn Ala Val 
65                  70                  75                  80  


Tyr Tyr Cys Ala Ser Thr Asp Ser Ser Ser Thr Ala Ala Arg 
                85                  90                  


<210>  20
<211>  112
<212>  PRT
<213>  Gallus gallus

<400>  20

Ala Leu Thr Gln Pro Ala Ser Val Ser Ala Asn Pro Gly Glu Thr Val 
1               5                   10                  15      


Lys Ile Thr Cys Ser Gly Gly Gly Ser Tyr Ala Gly Ser Tyr Tyr Tyr 
            20                  25                  30          


Gly Trp Tyr Gln Gln Lys Tyr Ala Gly Ser Tyr Tyr Tyr Gly Trp Tyr 
        35                  40                  45              


Gln Gln Lys Ser Pro Gly Ser Ala Pro Val Thr Leu Ile Tyr Asn Asn 
    50                  55                  60                  


Asn Asn Arg Pro Ser Asp Ile Pro Ser Arg Phe Ser Gly Ser Lys Ser 
65                  70                  75                  80  


Gly Ser Thr Ala Thr Leu Thr Ile Thr Gly Val Arg Ala Asp Asp Glu 
                85                  90                  95      


Ala Val Tyr Phe Cys Gly Ser Ala Asp Asn Ser Gly Ala Ala Phe Gly 
            100                 105                 110         


<210>  21
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  21
gggacacctg aaggcatgag tgg                                               23


<210>  22
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  22
ggcggaatcc cagcagctgt gt                                                22


<210>  23
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  23
gtgagtcgct gacctcgtct cggtc                                             25


<210>  24
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  24
gggctctttc tacctcaagg catgt                                             25


<210>  25
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide

<400>  25

Asp Tyr Lys Asp Glu 
1               5   


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  26
agcctcatta tccccctgat                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  27
tctctttccc ttcgctttga                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  28
acagttccgt ttccggtatg                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  29
cactccatcc tcttgctggt                                                   20


<210>  30
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide

<400>  30

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  31
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide

<400>  31

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  32
<211>  1548
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct encoding chimeric chicken-human Ig heavy chain

<400>  32
gccgtgacgt tggacgagtc cgggggcggc ctccagacgc ccgggggagc gctcagcctc       60

gtctgcaagg cctccgggtt caccttcagc agtaacgcca tgggttgggt gcgacaggcg      120

cccggcaagg ggctggagtg ggtcgctggt attgatgatg atggtagtgg cacaagatac      180

gcgccggcgg tgaagggccg tgccaccatc tcgagggaca acgggcagag cacactgagg      240

ctgcagctga acaacctcag ggctgaggac accggcatct actactgcac gaaatgtgct      300

tacagtagtg gttgtgatta tgaagctggt tatatcgacg catggggcca cgggaccgaa      360

gtcatcgtct cctccgcctc gaccaagggc ccatcggtct tccccctggc accctcctcc      420

aagagcacct ctgggggcac agcggccctg ggctgcctgg tcaaggacta cttccccgaa      480

ccggtgacgg tgtcgtggaa ctcaggcgcc ctgaccagcg gcgtgcacac cttcccggct      540

gtcctacagt cctcaggact ctactccctc agcagcgtgg tgaccgtgcc ctccagcagc      600

ttgggcaccc agacctacat ctgcaacgtg aatcacaagc ccagcaacac caaggtggac      660

aagaaagttg agcccaaatc ttgtgacaaa actcacacat gcccaccgtg cccagcacct      720

gaactcctgg ggggaccgtc agtcttcctc ttccccccaa aacccaagga caccctcatg      780

atctcccgga cccctgaggt cacatgcgtg gtggtggacg tgagccacga agaccctgag      840

gtcaagttca actggtacgt ggacggcgtg gaggtgcata atgccaagac aaagccgcgg      900

gaggagcagt acaacagcac gtaccgtgtg gtcagcgtcc tcaccgtcct gcaccaggac      960

tggctgaatg gcaaggagta caagtgcaag gtctccaaca aagccctccc agcccccatc     1020

gagaaaacca tctccaaagc caaagggcag ccccgagaac cacaggtgta caccctgccc     1080

ccatcccggg atgagctgac caagaaccag gtcagcctga cctgcctggt caaaggcttc     1140

tatcccagcg acatcgccgt ggagtgggag agcaatgggc agccggagaa caactacaag     1200

accacgcctc ccgtgctgga ctccgacggc tccttcttcc tctacagcaa gctcaccgtg     1260

gacaagagca ggtggcagca ggggaacgtc ttctcatgct ccgtgatgca tgaggctctg     1320

cacaaccact acacacagaa gagcctctcc ctgtctccgg gtaaagatta caaggatgac     1380

gacgataagg agaacctgta ttttcagggt gatctcctcc attggcctct ggaggccgaa     1440

gaggacgacg acatccaacg cctttgggcc accacctcca ccttcatcgt cctcttcatc     1500

ctcagcctct tctacagcgc caccgtcacc ctcatcaagg tgaaatga                  1548


<210>  33
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct encoding rearranged chicken IgH J region (chVDJ)

<400>  33
atcgacgcat ggggccacgg gaccgaagtc atcgtctcct ccg                         43


<210>  34
<211>  989
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct encoding human Ig C-gamma 1 region

<400>  34
cctcgaccaa gggcccatcg gtcttccccc tggcaccctc ctccaagagc acctctgggg       60

gcacagcggc cctgggctgc ctggtcaagg actacttccc cgaaccggtg acggtgtcgt      120

ggaactcagg cgccctgacc agcggcgtgc acaccttccc ggctgtccta cagtcctcag      180

gactctactc cctcagcagc gtggtgaccg tgccctccag cagcttgggc acccagacct      240

acatctgcaa cgtgaatcac aagcccagca acaccaaggt ggacaagaaa gttgagccca      300

aatcttgtga caaaactcac acatgcccac cgtgcccagc acctgaactc ctggggggac      360

cgtcagtctt cctcttcccc ccaaaaccca aggacaccct catgatctcc cggacccctg      420

aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt      480

acgtggacgg cgtggaggtg cataatgcca agacaaagcc gcgggaggag cagtacaaca      540

gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca ggactggctg aatggcaagg      600

agtacaagtg caaggtctcc aacaaagccc tcccagcccc catcgagaaa accatctcca      660

aagccaaagg gcagccccga gaaccacagg tgtacaccct gcccccatcc cgggatgagc      720

tgaccaagaa ccaggtcagc ctgacctgcc tggtcaaagg cttctatccc agcgacatcg      780

ccgtggagtg ggagagcaat gggcagccgg agaacaacta caagaccacg cctcccgtgc      840

tggactccga cggctccttc ttcctctaca gcaagctcac cgtggacaag agcaggtggc      900

agcaggggaa cgtcttctca tgctccgtga tgcatgaggc tctgcacaac cactacacac      960

agaagagcct ctccctgtct ccgggtaaa                                        989


<210>  35
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct encoding peptide epitope tag

<400>  35
gattacaagg atgacgacga taag                                              24


<210>  36
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct encoding protease cleavage site

<400>  36
gagaacctgt attttcaggg t                                                 21


<210>  37
<211>  138
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct encoding artificial chicken IgH transmembrane domain

<400>  37
gatctcctcc attggcctct ggaggccgaa gaggacgacg acatccaacg cctttgggcc       60

accacctcca ccttcatcgt cctcttcatc ctcagcctct tctacagcgc caccgtcacc      120

ctcatcaagg tgaaatga                                                    138


<210>  38
<211>  376
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct encoding rearranged chicken IgH J region (chVDJ)

<400>  38
gccgtgacgt tggacgagtc cgggggcggc ctccagacgc ccgggggagc gctcagcctc       60

gtctgcaagg cctccgggtt caccttcagc agtaacgcca tgggttgggt gcgacaggcg      120

cccggcaagg ggctggagtg ggtcgctggt attgatgatg atggtagtgg cacaagatac      180

gcgccggcgg tgaagggccg tgccaccatc tcgagggaca acgggcagag cacactgagg      240

ctgcagctga acaacctcag ggctgaggac accggcatct actactgcac gaaatgtgct      300

tacagtagtg gttgtgatta tgaagctggt tatatcgacg catggggcca cgggaccgaa      360

gtcatcgtct cctccg                                                      376


<210>  39
<211>  989
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct encoding human Ig C-gamma 1 region

<400>  39
cctcgaccaa gggcccatcg gtcttccccc tggcaccctc ctccaagagc acctctgggg       60

gcacagcggc cctgggctgc ctggtcaagg actacttccc cgaaccggtg acggtgtcgt      120

ggaactcagg cgccctgacc agcggcgtgc acaccttccc ggctgtccta cagtcctcag      180

gactctactc cctcagcagc gtggtgaccg tgccctccag cagcttgggc acccagacct      240

acatctgcaa cgtgaatcac aagcccagca acaccaaggt ggacaagaaa gttgagccca      300

aatcttgtga caaaactcac acatgcccac cgtgcccagc acctgaactc ctggggggac      360

cgtcagtctt cctcttcccc ccaaaaccca aggacaccct catgatctcc cggacccctg      420

aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt      480

acgtggacgg cgtggaggtg cataatgcca agacaaagcc gcgggaggag cagtacaaca      540

gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca ggactggctg aatggcaagg      600

agtacaagtg caaggtctcc aacaaagccc tcccagcccc catcgagaaa accatctcca      660

aagccaaagg gcagccccga gaaccacagg tgtacaccct gcccccatcc cgggatgagc      720

tgaccaagaa ccaggtcagc ctgacctgcc tggtcaaagg cttctatccc agcgacatcg      780

ccgtggagtg ggagagcaat gggcagccgg agaacaacta caagaccacg cctcccgtgc      840

tggactccga cggctccttc ttcctctaca gcaagctcac cgtggacaag agcaggtggc      900

agcaggggaa cgtcttctca tgctccgtga tgcatgaggc tctgcacaac cactacacac      960

agaagagcct ctccctgtct ccgggtaaa                                        989


<210>  40
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct encoding peptide epitope tag

<400>  40
gattacaagg atgacgacga taag                                              24


<210>  41
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct encoding protease cleavage site

<400>  41
gagaacctgt attttcaggg t                                                 21


<210>  42
<211>  138
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct encoding artificial chicken IgH transmembrane domain

<400>  42
gatctcctcc attggcctct ggaggccgaa gaggacgacg acatccaacg cctttgggcc       60

accacctcca ccttcatcgt cctcttcatc ctcagcctct tctacagcgc caccgtcacc      120

ctcatcaagg tgaaatga                                                    138


