                         SEQUENCE LISTING

<110>  Athenix Corp.
       Sampson, Kimberly
       Thayer, Rebecca
       Lehtinen, Duane
 
<120>  AXMI335 TOXIN GENE AND METHODS FOR ITS USE

<130>  2916693-098977

<150>  61/608,303
<151>  2012-03-08

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  2400
<212>  DNA
<213>  Bacillus thuringiensis

<400>  1
atgatgattg tggataataa taaattaaat gtaagagctt taccaagctt tattgattat       60

tttaacggta tttatggatt tgccactggt atcaaagata ttatgggaat gatttttaaa      120

acagatacag gtggtagtaa tttaacatta gatgagattt taaagaatca aaatttacta      180

aatgatatct cgggtaagct cgatggtatt aatggaggtt taggtgatct tattgcacaa      240

gggaacttaa attcagaatt agctaaggaa ttgctaaaaa tttctaatga gcagaatcag      300

atgttaaatc atgttaatgc tcaacttaat gcaatcaatt caacacttaa tgtatatctt      360

cccaaaatta catctatgtt aaatgaggtg atgatgcaaa accatgtttt aagtctacaa      420

atagaatttc taagtaaaca attgcaagaa atttcagata aacttgatat tatcaactta      480

aacgtactga ttaactctac attgacagag attactcctg cttatcaacg tattaaatat      540

gttaacgaaa aatttgatga attgacttct actgtagaga aaaattcaaa agcatatcaa      600

gataacgtta ctaaagaagt tattgaaaat ttaactgatc taactgaatt ggccaaaagt      660

gttacaaaaa atgatatgga tagttttgaa ttttatcttc aaactttcca tgatgtaatg      720

actggaaata atttatttgg tcgctcagca ttaaaaactg ctgcagaatt aatcacaaaa      780

gaaaatgtca cgacaagagg aagtgagata ggaaaagttt ataatttctt gattgtttta      840

acttctttac aagcaaaagc ttttctcact ttaactgcat gtcgaaagtt attgggttta      900

acagatattg attatactaa aactatgaat cagcatttag atggacaaaa aagagaattt      960

cgtattaata ttcttccaac actttctaat agtttttcta atcctagtta ttcaaagaat     1020

agaggaagtg atatcgatga tccaattgtt gtgttagaag cagcacctgg atatgcctta     1080

ataggatttc aaattctaaa cgatccactt ccgattttaa aaggatatca ggctaggtta     1140

aaaccaaatt atcaagttga cagagagtcg atgtcagaaa caatttatgg ggatattcat     1200

aaattatttt gtccaaaaca gctggagcaa aaatattata ttaaagatat tgaatttcct     1260

gaaggctatg taatcactaa aatcgtgttt gaaaaaaggc taaatcaatt gggttatgag     1320

gtaacggcaa atttttatga cccctctaca ggaaacatcg atttaaacaa ggttaaagta     1380

gaatcttgga aggaaaagtc ttgcgaggag gaatcctgcg aagatgagtt ctgcgaacat     1440

gagtatagcc ttataaaggc tgaaacggat ggtatttata tgccattagg tgtagtaagt     1500

gagacctttt taaccccaat ttatggtttt ggattaacag ttgacgaaaa aaatcaaaaa     1560

ataactttaa caggtaaatc ctatttacgt gaatccttac tagaaacaga tttagttaac     1620

aatgaaacat atttaattgc ttcaccagac ggttatttta gtagtattgt agaaaattgg     1680

aatataacat cagataattt tggatcttgg agagcaaata ataataatgc atttgtcgat     1740

aaggaagata ctgtaaaagg atcaagttct ctgtatactc ataaagatgg ggaattctcg     1800

caatttattg gaaataagct aaaacctaaa actaattatg ttattcaata tgctataaaa     1860

ggaagacccg ctatttattt aaaaaataat aaggatacct tgtttgagga taccaataac     1920

aactttagcg attttcagac tgtaacaaaa aaattcaatt caggagcaaa tccttcggaa     1980

atttatttgc tttttaaaaa tcaaggtgaa tacgaggctt gggggaataa ctttattatt     2040

ttagaaatta aatcgcttga attattgccg caaatgttga aacctgagga ttggatacca     2100

tcaggaaatg tgcaaatgaa agacgaagga cgcctagaga ttttaggaga tggctatttt     2160

aaacaattca ttaaattgca aaatgattca acctatcatc taagattatc agttaaggga     2220

accggtaggg tatcaataat tgatgaatct aactatttat tttttgtaaa tattaaggat     2280

gaagatttta ctagcgttat taaaaatagg tcttcagaag gtgattgttt tatagctctt     2340

gagggttctt atgtagaaaa ttctagtacc attttttcta gtgtatctat cgttaaagaa     2400


<210>  2
<211>  800
<212>  PRT
<213>  Bacillus thuringiensis

<400>  2

Met Met Ile Val Asp Asn Asn Lys Leu Asn Val Arg Ala Leu Pro Ser 
1               5                   10                  15      


Phe Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys 
            20                  25                  30          


Asp Ile Met Gly Met Ile Phe Lys Thr Asp Thr Gly Gly Ser Asn Leu 
        35                  40                  45              


Thr Leu Asp Glu Ile Leu Lys Asn Gln Asn Leu Leu Asn Asp Ile Ser 
    50                  55                  60                  


Gly Lys Leu Asp Gly Ile Asn Gly Gly Leu Gly Asp Leu Ile Ala Gln 
65                  70                  75                  80  


Gly Asn Leu Asn Ser Glu Leu Ala Lys Glu Leu Leu Lys Ile Ser Asn 
                85                  90                  95      


Glu Gln Asn Gln Met Leu Asn His Val Asn Ala Gln Leu Asn Ala Ile 
            100                 105                 110         


Asn Ser Thr Leu Asn Val Tyr Leu Pro Lys Ile Thr Ser Met Leu Asn 
        115                 120                 125             


Glu Val Met Met Gln Asn His Val Leu Ser Leu Gln Ile Glu Phe Leu 
    130                 135                 140                 


Ser Lys Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Ile Ile Asn Leu 
145                 150                 155                 160 


Asn Val Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro Ala Tyr Gln 
                165                 170                 175     


Arg Ile Lys Tyr Val Asn Glu Lys Phe Asp Glu Leu Thr Ser Thr Val 
            180                 185                 190         


Glu Lys Asn Ser Lys Ala Tyr Gln Asp Asn Val Thr Lys Glu Val Ile 
        195                 200                 205             


Glu Asn Leu Thr Asp Leu Thr Glu Leu Ala Lys Ser Val Thr Lys Asn 
    210                 215                 220                 


Asp Met Asp Ser Phe Glu Phe Tyr Leu Gln Thr Phe His Asp Val Met 
225                 230                 235                 240 


Thr Gly Asn Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr Ala Ala Glu 
                245                 250                 255     


Leu Ile Thr Lys Glu Asn Val Thr Thr Arg Gly Ser Glu Ile Gly Lys 
            260                 265                 270         


Val Tyr Asn Phe Leu Ile Val Leu Thr Ser Leu Gln Ala Lys Ala Phe 
        275                 280                 285             


Leu Thr Leu Thr Ala Cys Arg Lys Leu Leu Gly Leu Thr Asp Ile Asp 
    290                 295                 300                 


Tyr Thr Lys Thr Met Asn Gln His Leu Asp Gly Gln Lys Arg Glu Phe 
305                 310                 315                 320 


Arg Ile Asn Ile Leu Pro Thr Leu Ser Asn Ser Phe Ser Asn Pro Ser 
                325                 330                 335     


Tyr Ser Lys Asn Arg Gly Ser Asp Ile Asp Asp Pro Ile Val Val Leu 
            340                 345                 350         


Glu Ala Ala Pro Gly Tyr Ala Leu Ile Gly Phe Gln Ile Leu Asn Asp 
        355                 360                 365             


Pro Leu Pro Ile Leu Lys Gly Tyr Gln Ala Arg Leu Lys Pro Asn Tyr 
    370                 375                 380                 


Gln Val Asp Arg Glu Ser Met Ser Glu Thr Ile Tyr Gly Asp Ile His 
385                 390                 395                 400 


Lys Leu Phe Cys Pro Lys Gln Leu Glu Gln Lys Tyr Tyr Ile Lys Asp 
                405                 410                 415     


Ile Glu Phe Pro Glu Gly Tyr Val Ile Thr Lys Ile Val Phe Glu Lys 
            420                 425                 430         


Arg Leu Asn Gln Leu Gly Tyr Glu Val Thr Ala Asn Phe Tyr Asp Pro 
        435                 440                 445             


Ser Thr Gly Asn Ile Asp Leu Asn Lys Val Lys Val Glu Ser Trp Lys 
    450                 455                 460                 


Glu Lys Ser Cys Glu Glu Glu Ser Cys Glu Asp Glu Phe Cys Glu His 
465                 470                 475                 480 


Glu Tyr Ser Leu Ile Lys Ala Glu Thr Asp Gly Ile Tyr Met Pro Leu 
                485                 490                 495     


Gly Val Val Ser Glu Thr Phe Leu Thr Pro Ile Tyr Gly Phe Gly Leu 
            500                 505                 510         


Thr Val Asp Glu Lys Asn Gln Lys Ile Thr Leu Thr Gly Lys Ser Tyr 
        515                 520                 525             


Leu Arg Glu Ser Leu Leu Glu Thr Asp Leu Val Asn Asn Glu Thr Tyr 
    530                 535                 540                 


Leu Ile Ala Ser Pro Asp Gly Tyr Phe Ser Ser Ile Val Glu Asn Trp 
545                 550                 555                 560 


Asn Ile Thr Ser Asp Asn Phe Gly Ser Trp Arg Ala Asn Asn Asn Asn 
                565                 570                 575     


Ala Phe Val Asp Lys Glu Asp Thr Val Lys Gly Ser Ser Ser Leu Tyr 
            580                 585                 590         


Thr His Lys Asp Gly Glu Phe Ser Gln Phe Ile Gly Asn Lys Leu Lys 
        595                 600                 605             


Pro Lys Thr Asn Tyr Val Ile Gln Tyr Ala Ile Lys Gly Arg Pro Ala 
    610                 615                 620                 


Ile Tyr Leu Lys Asn Asn Lys Asp Thr Leu Phe Glu Asp Thr Asn Asn 
625                 630                 635                 640 


Asn Phe Ser Asp Phe Gln Thr Val Thr Lys Lys Phe Asn Ser Gly Ala 
                645                 650                 655     


Asn Pro Ser Glu Ile Tyr Leu Leu Phe Lys Asn Gln Gly Glu Tyr Glu 
            660                 665                 670         


Ala Trp Gly Asn Asn Phe Ile Ile Leu Glu Ile Lys Ser Leu Glu Leu 
        675                 680                 685             


Leu Pro Gln Met Leu Lys Pro Glu Asp Trp Ile Pro Ser Gly Asn Val 
    690                 695                 700                 


Gln Met Lys Asp Glu Gly Arg Leu Glu Ile Leu Gly Asp Gly Tyr Phe 
705                 710                 715                 720 


Lys Gln Phe Ile Lys Leu Gln Asn Asp Ser Thr Tyr His Leu Arg Leu 
                725                 730                 735     


Ser Val Lys Gly Thr Gly Arg Val Ser Ile Ile Asp Glu Ser Asn Tyr 
            740                 745                 750         


Leu Phe Phe Val Asn Ile Lys Asp Glu Asp Phe Thr Ser Val Ile Lys 
        755                 760                 765             


Asn Arg Ser Ser Glu Gly Asp Cys Phe Ile Ala Leu Glu Gly Ser Tyr 
    770                 775                 780                 


Val Glu Asn Ser Ser Thr Ile Phe Ser Ser Val Ser Ile Val Lys Glu 
785                 790                 795                 800 


<210>  3
<211>  2397
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic nucleotide sequence encoding Axmi335

<400>  3
atgattgtgg acaacaacaa gctcaatgtc cgcgcgctgc catccttcat cgactacttc       60

aatggcatct atggcttcgc caccggcatc aaggacatca tggggatgat cttcaagaca      120

gacactggag gatcaaacct caccttggat gagatcctca agaaccagaa cctgctgaat      180

gacatcagcg gcaagctgga tggcatcaat ggaggccttg gagatctgat tgctcaagga      240

aacctcaact cagagctggc caaggagctg ctgaagatca gcaatgagca gaaccagatg      300

ctgaaccatg tcaatgctca gctcaatgcc atcaacagca ccctcaatgt ttatcttcca      360

aagatcacct caatgctgaa tgaggtgatg atgcaaaatc atgtgctgag cctccagatc      420

gagttcctct ccaagcagct gcaagagatc tccgacaagc tggacatcat caacctcaat      480

gtgctgatca actcaacatt gacagagatc acgccggcct accagaggat caaatatgtc      540

aatgagaagt ttgatgagct gacaagcacc gtggagaaga acagcaaggc ctaccaggac      600

aatgtcacca aggaggtgat tgagaacctg acagatctga cagagctggc aaaatcagtc      660

accaagaatg acatggacag cttcgagttc tacctccaga ccttccatga tgtgatgaca      720

ggcaacaacc tctttggaag aagcgcgctg aagacagcag cagagctgat caccaaggag      780

aatgtgacaa caagaggatc agagattggc aaggtgtaca acttcctcat cgtgctgaca      840

agcctccagg ccaaggcctt cctcaccctc accgcctgcc gaaagctgct gggcctcact      900

gacatcgact acaccaagac catgaaccag catctggatg gccagaagag ggagttcaga      960

atcaacatcc tgccaacatt gagcaacagc ttcagcaacc caagctacag caagaacaga     1020

ggatcagaca ttgatgatcc aattgtggtg ctggaagctg ctcctggata tgctctcatc     1080

ggcttccaga tcctcaatga tcctcttccc atcctcaaag gatatcaagc aaggctgaag     1140

ccaaactacc aggtggacag agaaagcatg tcagaaacca tctatggaga catccacaag     1200

ctcttctgcc ccaagcagct ggagcagaag tactacatca aggacatcga gttcccagaa     1260

ggatatgtca tcaccaagat cgtcttcgag aagaggctga accagctggg atatgaggtc     1320

accgccaact tctatgatcc atcaactggc aacatcgacc tcaacaaggt gaaggtggag     1380

agctggaagg agaagagctg cgaggaggag agctgtgaag atgagttctg cgagcatgag     1440

tacagcttga tcaaggcaga aacagatggc atctacatgc cgctcggcgt ggtttcagaa     1500

accttcttga cgcccatcta tggcttcggc ctcaccgtgg atgagaagaa ccagaagatc     1560

accttgacag ggaagagcta cctgagggag agcttgctgg agacagatct ggtgaacaat     1620

gaaacatatt tgattgcttc tccagatggc tacttcagca gcatcgtgga gaactggaac     1680

atcacctcag acaactttgg aagctggagg gccaacaaca acaatgcttt tgtggacaag     1740

gaggacaccg tcaaaggaag cagcagcctc tacacccaca aggatggaga gttctcacag     1800

ttcattggaa ataagctgaa gccaaagacc aactatgtca tccaatatgc catcaaagga     1860

aggccggcca tctacctgaa gaacaacaag gacaccctct tcgaggacac caacaacaac     1920

ttctccgact tccaaactgt caccaagaag ttcaacagcg gcgccaaccc ttcagagatc     1980

tacctgctct tcaagaatca aggagaatat gaagcatggg gcaacaactt catcatcctg     2040

gagatcaaga gcttggagct gctgccgcag atgctgaagc cagaagattg gattccatca     2100

ggaaatgttc agatgaagga tgaaggaagg ctggagatcc ttggagatgg ctacttcaag     2160

cagttcatca agctgcaaaa tgacagcacc taccacctcc gcctctccgt caaaggaact     2220

ggccgcgtct ccatcattga tgaaagcaac tacctcttct tcgtgaacat caaggatgaa     2280

gatttcacct ccgtcatcaa gaacagaagc tcagaaggag attgcttcat cgcgctggaa     2340

ggaagctatg tggagaacag cagcaccatc ttctcctccg tctccatcgt caaggaa        2397


<210>  4
<211>  799
<212>  PRT
<213>  Bacillus thuringiensis

<400>  4

Met Ile Val Asp Asn Asn Lys Leu Asn Val Arg Ala Leu Pro Ser Phe 
1               5                   10                  15      


Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys Asp 
            20                  25                  30          


Ile Met Gly Met Ile Phe Lys Thr Asp Thr Gly Gly Ser Asn Leu Thr 
        35                  40                  45              


Leu Asp Glu Ile Leu Lys Asn Gln Asn Leu Leu Asn Asp Ile Ser Gly 
    50                  55                  60                  


Lys Leu Asp Gly Ile Asn Gly Gly Leu Gly Asp Leu Ile Ala Gln Gly 
65                  70                  75                  80  


Asn Leu Asn Ser Glu Leu Ala Lys Glu Leu Leu Lys Ile Ser Asn Glu 
                85                  90                  95      


Gln Asn Gln Met Leu Asn His Val Asn Ala Gln Leu Asn Ala Ile Asn 
            100                 105                 110         


Ser Thr Leu Asn Val Tyr Leu Pro Lys Ile Thr Ser Met Leu Asn Glu 
        115                 120                 125             


Val Met Met Gln Asn His Val Leu Ser Leu Gln Ile Glu Phe Leu Ser 
    130                 135                 140                 


Lys Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Ile Ile Asn Leu Asn 
145                 150                 155                 160 


Val Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro Ala Tyr Gln Arg 
                165                 170                 175     


Ile Lys Tyr Val Asn Glu Lys Phe Asp Glu Leu Thr Ser Thr Val Glu 
            180                 185                 190         


Lys Asn Ser Lys Ala Tyr Gln Asp Asn Val Thr Lys Glu Val Ile Glu 
        195                 200                 205             


Asn Leu Thr Asp Leu Thr Glu Leu Ala Lys Ser Val Thr Lys Asn Asp 
    210                 215                 220                 


Met Asp Ser Phe Glu Phe Tyr Leu Gln Thr Phe His Asp Val Met Thr 
225                 230                 235                 240 


Gly Asn Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr Ala Ala Glu Leu 
                245                 250                 255     


Ile Thr Lys Glu Asn Val Thr Thr Arg Gly Ser Glu Ile Gly Lys Val 
            260                 265                 270         


Tyr Asn Phe Leu Ile Val Leu Thr Ser Leu Gln Ala Lys Ala Phe Leu 
        275                 280                 285             


Thr Leu Thr Ala Cys Arg Lys Leu Leu Gly Leu Thr Asp Ile Asp Tyr 
    290                 295                 300                 


Thr Lys Thr Met Asn Gln His Leu Asp Gly Gln Lys Arg Glu Phe Arg 
305                 310                 315                 320 


Ile Asn Ile Leu Pro Thr Leu Ser Asn Ser Phe Ser Asn Pro Ser Tyr 
                325                 330                 335     


Ser Lys Asn Arg Gly Ser Asp Ile Asp Asp Pro Ile Val Val Leu Glu 
            340                 345                 350         


Ala Ala Pro Gly Tyr Ala Leu Ile Gly Phe Gln Ile Leu Asn Asp Pro 
        355                 360                 365             


Leu Pro Ile Leu Lys Gly Tyr Gln Ala Arg Leu Lys Pro Asn Tyr Gln 
    370                 375                 380                 


Val Asp Arg Glu Ser Met Ser Glu Thr Ile Tyr Gly Asp Ile His Lys 
385                 390                 395                 400 


Leu Phe Cys Pro Lys Gln Leu Glu Gln Lys Tyr Tyr Ile Lys Asp Ile 
                405                 410                 415     


Glu Phe Pro Glu Gly Tyr Val Ile Thr Lys Ile Val Phe Glu Lys Arg 
            420                 425                 430         


Leu Asn Gln Leu Gly Tyr Glu Val Thr Ala Asn Phe Tyr Asp Pro Ser 
        435                 440                 445             


Thr Gly Asn Ile Asp Leu Asn Lys Val Lys Val Glu Ser Trp Lys Glu 
    450                 455                 460                 


Lys Ser Cys Glu Glu Glu Ser Cys Glu Asp Glu Phe Cys Glu His Glu 
465                 470                 475                 480 


Tyr Ser Leu Ile Lys Ala Glu Thr Asp Gly Ile Tyr Met Pro Leu Gly 
                485                 490                 495     


Val Val Ser Glu Thr Phe Leu Thr Pro Ile Tyr Gly Phe Gly Leu Thr 
            500                 505                 510         


Val Asp Glu Lys Asn Gln Lys Ile Thr Leu Thr Gly Lys Ser Tyr Leu 
        515                 520                 525             


Arg Glu Ser Leu Leu Glu Thr Asp Leu Val Asn Asn Glu Thr Tyr Leu 
    530                 535                 540                 


Ile Ala Ser Pro Asp Gly Tyr Phe Ser Ser Ile Val Glu Asn Trp Asn 
545                 550                 555                 560 


Ile Thr Ser Asp Asn Phe Gly Ser Trp Arg Ala Asn Asn Asn Asn Ala 
                565                 570                 575     


Phe Val Asp Lys Glu Asp Thr Val Lys Gly Ser Ser Ser Leu Tyr Thr 
            580                 585                 590         


His Lys Asp Gly Glu Phe Ser Gln Phe Ile Gly Asn Lys Leu Lys Pro 
        595                 600                 605             


Lys Thr Asn Tyr Val Ile Gln Tyr Ala Ile Lys Gly Arg Pro Ala Ile 
    610                 615                 620                 


Tyr Leu Lys Asn Asn Lys Asp Thr Leu Phe Glu Asp Thr Asn Asn Asn 
625                 630                 635                 640 


Phe Ser Asp Phe Gln Thr Val Thr Lys Lys Phe Asn Ser Gly Ala Asn 
                645                 650                 655     


Pro Ser Glu Ile Tyr Leu Leu Phe Lys Asn Gln Gly Glu Tyr Glu Ala 
            660                 665                 670         


Trp Gly Asn Asn Phe Ile Ile Leu Glu Ile Lys Ser Leu Glu Leu Leu 
        675                 680                 685             


Pro Gln Met Leu Lys Pro Glu Asp Trp Ile Pro Ser Gly Asn Val Gln 
    690                 695                 700                 


Met Lys Asp Glu Gly Arg Leu Glu Ile Leu Gly Asp Gly Tyr Phe Lys 
705                 710                 715                 720 


Gln Phe Ile Lys Leu Gln Asn Asp Ser Thr Tyr His Leu Arg Leu Ser 
                725                 730                 735     


Val Lys Gly Thr Gly Arg Val Ser Ile Ile Asp Glu Ser Asn Tyr Leu 
            740                 745                 750         


Phe Phe Val Asn Ile Lys Asp Glu Asp Phe Thr Ser Val Ile Lys Asn 
        755                 760                 765             


Arg Ser Ser Glu Gly Asp Cys Phe Ile Ala Leu Glu Gly Ser Tyr Val 
    770                 775                 780                 


Glu Asn Ser Ser Thr Ile Phe Ser Ser Val Ser Ile Val Lys Glu 
785                 790                 795                 


<210>  5
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  endoplasmic reticulum targeting peptide

<400>  5

Lys Asp Glu Leu 
1               


