                         SEQUENCE LISTING

<110>  Vela Operations, Pte. Ltd
 
<120>  Real-time PCR detection of Mycobacterium tuberculosis complex

<130>  V 8929 LB

<150>  GB1203903.8
<151>  2012-03-06

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  16
<212>  DNA
<213>  Mycobacterium tuberculosis

<400>  1
gaccgtgcgg atcttg                                                       16


<210>  2
<211>  18
<212>  DNA
<213>  Mycobacterium tuberculosis

<400>  2
gctgcacatg gagatcac                                                     18


<210>  3
<211>  24
<212>  DNA
<213>  Mycobacterium tuberculosis

<400>  3
cggatttcgg tgccgtcgtc tttg                                              24


<210>  4
<211>  19
<212>  DNA
<213>  Mycobacterium tuberculosis

<400>  4
gtgcagaagg tctgtaacg                                                    19


<210>  5
<211>  18
<212>  DNA
<213>  Mycobacterium tuberculosis

<400>  5
ggacacagcc ttgttcac                                                     18


<210>  6
<211>  17
<212>  DNA
<213>  Mycobacterium tuberculosis

<400>  6
caccgacgcg aaagtcg                                                      17


<210>  7
<211>  23
<212>  DNA
<213>  Lactococcus lactis

<400>  7
ccttaggtat tcgtatggtt gac                                               23


<210>  8
<211>  18
<212>  DNA
<213>  Lactococcus lactis

<400>  8
acccgcttga acagagta                                                     18


<210>  9
<211>  25
<212>  DNA
<213>  Lactococcus lactis

<400>  9
caaccactcc accagttacg ctgct                                             25


<210>  10
<211>  1962
<212>  DNA
<213>  Mycobacterium tuberculosis complex

<400>  10
tcacttcttg cctttgtccc cggcggcatc ggtggacaat gccgcgacga aagcctcctg       60

tggcacctcg acgcgcccga tggtcttcat ccgcttcttg ccttccttct gcttctccag      120

cagcttgcgt ttgcgcgtga tgtcgccgcc gtagcacttg gacaacacgt ccttgcggat      180

cgcgcggatg ttttcgcggg caatgatttt cgatccgatg gcggcctgca ccggcacctc      240

gaactgctgg cgcgggatca gctccttgag tttggtggtc atcttgttgc cgtaggcata      300

cgccgtgtcc ttgtgcacga tcgcgctgaa cgcatccacc gcctcgccct gcagcaggat      360

gtcgaccttg accagcgcgg cctcctgttc gccggcctcc tcgtagtcga ggctggcata      420

gccgcgggtg cgcgatttca gtgcgtcgaa gaagtcgaag atgatctcgc cgagcggcat      480

ggtgtagcgc agttccaccc gctcggggga gagatagtcc atgccgccca actcgccgcg      540

gcgcgactgg cacagctcca tgatggtgcc gatgaactcg ctgggcgcga tgatggtggt      600

cttgacgacg ggctcgtaga ccgtgcggat cttgccctcc ggccagtccg acggattggt      660

cacccggatt tcggtgccgt cgtctttgtg cacccgatac accacattgg gtgaggtcga      720

gatcaggtcc aggccgaact cgcgctcaag gcgctcacgg gtgatctcca tgtgcagcag      780

gcccaagaaa ccgcaccgga acccaaaacc cagcgccacc gaggtttccg gctcataggt      840

caaggccgcg tcgttgagct gcagcttgtc cagggcgtcg cgcaggttcg ggtagtccga      900

accgtcgacc ggatacaacc ccgagtagac catcggtttg ggctcacggt agccggtcaa      960

cgcttcggcg gcagccccgc gggcccggga gaggctggtc acggtgtcgc ccaccttgga     1020

ctggcggacg tccttgacgc cggtgatcag gtaacccacc tcgccgacac cgaggccctc     1080

acacggtttc ggctcgggtg agacgatgcc gacctcaagc agctcgtggg tggcgccggt     1140

ggacatcatc atgatgcgct cacgggggct gatcttgccg tcgacgacgc ggacgtaggt     1200

caccactccg cggtagatgt cgtaaacgga gtcgaaaatc attgcgcggg taggtgcctc     1260

ggcgtcgccc tgagggggcg gcacctgtcg gaccacctcg tcgagcaggt cggacacgcc     1320

ttcgccggtt ttgccggaca cccgcaacac ctcggccggc tcgcagccga tgatgtgtgc     1380

catctcggcg gcgtaacggt ccgggtcggc cgcgggcagg tcgatcttgt tgagcaccgg     1440

gatgatgtgc aggtcgcggt ccaacgccag gtagaggttc gccagcgtct gcgcctcgat     1500

gccttgcgcg gcatcgacca acagcaccgc accctcgcaa gcctccagcg cacgcgagac     1560

ttcgtaggtg aagtcgacat ggcccggggt gtcgatcaga tgcagcacgt agtcggtctt     1620

gtcgacccgc cagggtagcc gcacattctg ggccttgatg gtgatgccgc gttcccgctc     1680

gatgtccatc cgatccaagt actgggcccg catagagcgt tcgtcgacca cgccggtgag     1740

ctgcagcatc cggtcggcca acgttgactt gccgtggtcg atgtgggcga tgatgcaaaa     1800

gttcctaatc tgcgccggcg cagtgaaggt tttgtcggcg aaactgctga tgggaatctc     1860

ctggagcggg ggttgacggg tatccagggt atccgcgtcg ggcagctgcg acccaatcgc     1920

gctcggtcga tcgcgtctat gctgcgagca tggcgtccgc ac                        1962


<210>  11
<211>  2145
<212>  DNA
<213>  Mycobacterium tuberculosis

<400>  11
atgggtaaaa acgaggccag aagatcggcc ctggcgcccg atcacggtac agtggtgtgc       60

gaccccctgc ggcgactcaa ccgcatgcac gcaacccctg aggagagtat tcggatcgtg      120

gctgcccaga aaaagaaggc ccaagacgaa tacggcgctg cgtctatcac cattctcgaa      180

gggctggagg ccgtccgcaa acgtcccggc atgtacattg gctcgaccgg tgagcgcggt      240

ttacaccatc tcatttggga ggtggtcgac aacgcggtcg acgaggcgat ggccggttat      300

gcaaccacag tgaacgtagt gctgcttgag gatggcggtg tcgaggtcgc cgacgacggc      360

cgcggcattc cggtcgccac ccacgcctcc ggcataccga ccgtcgacgt ggtgatgaca      420

caactacatg ccggcggcaa gttcgactcg gacgcgtatg cgatatctgg tggtctgcac      480

ggcgtcggcg tgtcggtggt taacgcgcta tccacccggc tcgaagtcga gatcaagcgc      540

gacgggtacg agtggtctca ggtttatgag aagtcggaac ccctgggcct caagcaaggg      600

gcgccgacca agaagacggg gtcaacggtg cggttctggg ccgaccccgc tgttttcgaa      660

accacggaat acgacttcga aaccgtcgcc cgccggctgc aagagatggc gttcctcaac      720

aaggggctga ccatcaacct gaccgacgag agggtgaccc aagacgaggt cgtcgacgaa      780

gtggtcagcg acgtcgccga ggcgccgaag tcggcaagtg aacgcgcagc cgaatccact      840

gcaccgcaca aagttaagag ccgcaccttt cactatccgg gtggcctggt ggacttcgtg      900

aaacacatca accgcaccaa gaacgcgatt catagcagca tcgtggactt ttccggcaag      960

ggcaccgggc acgaggtgga gatcgcgatg caatggaacg ccgggtattc ggagtcggtg     1020

cacaccttcg ccaacaccat caacacccac gagggcggca cccacgaaga gggcttccgc     1080

agcgcgctga cgtcggtggt gaacaagtac gccaaggacc gcaagctact gaaggacaag     1140

gaccccaacc tcaccggtga cgatatccgg gaaggcctgg ccgctgtgat ctcggtgaag     1200

gtcagcgaac cgcagttcga gggccagacc aagaccaagt tgggcaacac cgaggtcaaa     1260

tcgtttgtgc agaaggtctg taacgaacag ctgacccact ggtttgaagc caaccccacc     1320

gacgcgaaag tcgttgtgaa caaggctgtg tcctcggcgc aagcccgtat cgcggcacgt     1380

aaggcacgag agttggtgcg gcgtaagagc gccaccgaca tcggtggatt gcccggcaag     1440

ctggccgatt gccgttccac ggatccgcgc aagtccgaac tgtatgtcgt agaaggtgac     1500

tcggccggcg gttctgcaaa aagcggtcgc gattcgatgt tccaggcgat acttccgctg     1560

cgcggcaaga tcatcaatgt ggagaaagcg cgcatcgacc gggtgctaaa gaacaccgaa     1620

gttcaggcga tcatcacggc gctgggcacc gggatccacg acgagttcga tatcggcaag     1680

ctgcgctacc acaagatcgt gctgatggcc gacgccgatg ttgacggcca acatatttcc     1740

acgctgttgt tgacgttgtt gttccggttc atgcggccgc tcatcgagaa cgggcatgtg     1800

tttttggcac aaccgccgct gtacaaactc aagtggcagc gcagtgaccc ggaattcgca     1860

tactccgacc gcgagcgcga cggtctgctg gaggcggggc tgaaggccgg gaagaagatc     1920

aacaaggaag acggcattca gcggtacaag ggtctaggtg aaatggacgc taaggagttg     1980

tgggagacca ccatggatcc ctcggttcgt gtgttgcgtc aagtgacgct ggacgacgcc     2040

gccgccgccg acgagttgtt ctccatcctg atgggcgagg acgtcgacgc gcggcgcagc     2100

tttatcaccc gcaacgccaa ggatgttcgg ttcctggatg tctaa                     2145


<210>  12
<211>  20
<212>  DNA
<213>  Mycobacterium tuberculosis

<400>  12
cgtagaccgt gcggatcttg                                                   20


<210>  13
<211>  21
<212>  DNA
<213>  Mycobacterium tuberculosis

<400>  13
cctgctgcac atggagatca c                                                 21


<210>  14
<211>  22
<212>  DNA
<213>  Mycobacterium tuberculosis

<400>  14
gcagaaggtc tgtaacgaac ag                                                22


<210>  15
<211>  21
<212>  DNA
<213>  Mycobacterium tuberculosis

<400>  15
cgaggacaca gccttgttca c                                                 21


<210>  16
<211>  23
<212>  DNA
<213>  Plasmid based on pUC57 serving as extraction control

<400>  16
aattcctcag ggattattgt ggc                                               23


<210>  17
<211>  24
<212>  DNA
<213>  Plasmid based on pUC57 serving as extraction control

<400>  17
cacaaccagt atgcaacatc aacc                                              24


<210>  18
<211>  25
<212>  DNA
<213>  Probe to detect amplification product in Plasmid based on pUC57 serving as extraction control

<400>  18
aacctcttga actgacagcg tgtgc                                             25


<210>  19
<211>  527
<212>  DNA
<213>  Plasmid comprising lepA sequence of MTC

<400>  19
gcggccgcct ggggttgggg tggagtctct gacggcttct tccgtcttga cgcactaaac       60

ccttcagctc ttggtactgg tggcggcgca ggcggcttca acggttacca aagtgctgtt      120

gtaggcatca aaccttagta tggaaatgca tggtggtctt gacgacgggc tcgtagaccg      180

tgcggatctt gccctccggc cagtccgacg gattggtcac ccggatttcg gtgccgtcgt      240

ctttgtgcac ccgatacacc acattgggtg aggtcgagat caggtccagg ccgaactcgc      300

gctcaaggcg ctcacgggtg atctccatgt gcagcaggcc caagaaaccg caccggaacc      360

cgccagcagg gctcttcgtc agctcccact cgtagccgta caggatctcg aggaaactgt      420

tgtcccattt cgtcggggtg ttcgtccata cgacctcgat gccggtggtg atcgcgtcct      480

taccggttcc ggtgccatac gagctcttcc agcccaagcc catctgc                    527


<210>  20
<211>  403
<212>  DNA
<213>  Plasmid comprising gyrB sequence

<400>  20
gcggccgcgg gcaacaccga ggtcaaatcg tttgtgcaga aggtctgtaa cgaacagctg       60

acccactggt ttgaagccaa ccccaccgac gcgaaagtcg ttgtgaacaa ggctgtgtcc      120

tcggcgcaag cccgtatcgc ggcacgtaag gcacgagagt tggtgcggcg taagagcgcc      180

accgacatga ccacccagga cgtggaggcg atcacaccgc agacgttgat caacatccgg      240

ccggtggtcg ccgcgatcaa ggagttcttc ggcaccagcc agctgagcca attcatggac      300

cagaacaacc cgctgtcggg gttgacccac aagcgccgac tgtcggcgct ggggcccggc      360

ggtctgtcac gtgagcgtgc cgggctggag gtccgcgacg tgc                        403


<210>  21
<211>  443
<212>  DNA
<213>  Plasmid based on pUC57 serving as extraction control

<400>  21
gctaatacga ctcactatag ggagatagca tttaggtgac actatagaag ttagtacctg       60

ggaagcaagc cgcggaaatg atcagaagac gtgcgaattc ctcagggatt attgtggcca      120

cgaaggataa cgttaaaacc gttgattctt tcatgatgaa ttttgggaaa agcacacgct      180

gtcagttcaa gaggttattc attgatgaag ggttgatgtt gcatactggt tgtgttaatt      240

ttcttgtggc gatgtcattg tggtaagatt tcacgtcctg ccttaggtat tcgtatggtt      300

gacctatctc aattatcaac aaatgatagt tctcaactga aattacctag cagcgtaact      360

ggtggagtgg ttgtctactc tgttcaagcg ggtcttcctg ctgccacagc tggaaaaaaa      420

aaaaaaaaaa aaaaaaaaaa aaa                                              443


