                         SEQUENCE LISTING

<110>  Winthrop-University Hospital
       Akirav, Eitan M.
 
<120>  METHOD FOR USING PROBE BASED PCR DETECTION TO MEASURE THE LEVELS 
       OF CIRCULATING DEMETHYLATED BETA CELL DERIVED DNA AS A MEASURE OF
        BETA CELL LOSS IN DIABETES

<130>  P/2526-132

<150>  61/606140
<151>  2012-03-02

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward PCR Primer for insulin gene

<400>  1
gtgcggttta tatttggtgg aagtt                                             25


<210>  2
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse PCR primer for insulin gene

<400>  2
acaacaataa acaattaact caccctacaa                                        30


<210>  3
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe for the detection of methylated non-beta cell insulin DNA

<400>  3
acctcccgac gaatct                                                       16


<210>  4
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe for the detection of methylated non-beta cell insulin DNA

<400>  4
tacctctcgt cgaatct                                                      17


<210>  5
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe for the detection of demethylated beta cell insulin DNA

<400>  5
acctcccaac aaatct                                                       16


<210>  6
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe for the detection of demethylated beta cell insulin dna

<400>  6
tacctcccat caaatct                                                      17


<210>  7
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Outer Forward PCR primer for nested PCR according to Akirav et 
       al. (2011)

<400>  7
ttaggggttt taaggtaggg tatttggt                                          28


<210>  8
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Outer Reverse PCR primer for nested PCR according to Akirav et 
       al. (2011)

<400>  8
accaaaaaca acaataaaca attaactcac cctacaa                                37


<210>  9
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Inner Methylated Forward PCR primer for nested PCR according to 
       Akirav et al. (2011)

<400>  9
gtggatgcgt tttttgtttt tgttggc                                           27


<210>  10
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Inner Methylated Reverse PCR primer for nested PCR according to 
       Akirav et al. (2011)

<400>  10
caccctacaa atcctctacc tcccg                                             25


<210>  11
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Inner Demethylated Forward PCR primer for nested PCR according to
       Akirav et al. (2011)

<400>  11
ttgtggatgt gttttttgtt tttgttggt                                         29


<210>  12
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Inner Demethylated Reverse PCR primer for nested PCR according to
       Akirav et al. (2011)

<400>  12
caccctacaa atcctctacc tccca                                             25


