                         SEQUENCE LISTING

<110>  LUDWIG INSTITUTE FOR CANCER RESEARCH LTD.
       CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD
 
<120>  iASPP PHOSPHORYLATION AND METASTATIC POTENTIAL

<130>  31265/46773B

<150>  US-61/606,090
<151>  2012-03-02

<150>  US-61/711,616
<151>  2012-10-09

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  2487
<212>  DNA
<213>  Homo sapiens

<400>  1
atggacagcg aggcattcca gagcgcgcgg gactttctgg acatgaactt ccagtcgctg       60

gccatgaaac acatggatct gaagcagatg gagctggaca cggcggcggc caaggtggat      120

gaactgacca agcagctgga gtcgctgtgg tcagactctc ccgcgcctcc tggcccgcag      180

gccggacccc cttctaggcc gccccggtac agctccagct cgatccctga gcccttcggc      240

agccgagggt ccccccggaa ggcggccacc gacggcgcag acaccccgtt cggacgatca      300

gagagtgccc caaccctaca cccctacagc ccgctgtccc ccaagggacg gccgtcgtcg      360

ccgcgcaccc cgctctacct gcagccggac gcctacggca gcctggaccg cgcgacctcg      420

ccccggcccc gcgccttcga tggcgcaggc agctccctcg gccgtgcgcc ctccccgcgg      480

cccgggccag gcccgctccg ccagcagggt ccccccacgc ctttcgactt cctgggccgc      540

gcaggctccc cccgcggcag ccccctggcg gaggggcccc aggccttctt ccccgagcgt      600

gggccgtcac cgcgcccccc tgccacagcc tacgacgcgc cagcgtccgc cttcgggagc      660

tccctgctag gctccggcgg cagcgcattc gccccgcctc tgcgcgcgca agacgacctg      720

acgctgcgcc ggcggcctcc gaaagcctgg aacgagtctg acctggacgt ggcgtacgag      780

aagaagcctt cgcagacagc gagctatgaa cgcctggacg tcttcgcaag gcctgcctcg      840

ccgagcctgc agctgttgcc ttggagggag agcagcctgg atggactggg gggcaccggc      900

aaggacaacc tcactagcgc caccctgccg cgcaattaca aggtctctcc tctggccagc      960

gaccggcgtt cagacgcggg cagctaccgg cgctcgctgg gctccgcggg gccgtcgggc     1020

actttgcctc gcagctggca gcccgtcagc cgcatcccca tgcccccctc cagcccccag     1080

ccccgcgggg ccccgcgcca gcgtcccatc cccctcagca tgatcttcaa gctgcagaac     1140

gccttctggg agcacggggc cagccgcgcc atgctccctg ggtcccccct cttcacccga     1200

gcacccccgc ctaagctgca gccccaacca caaccacagc cccagccaca atcacaacca     1260

cagccccagc tgcccccaca gccccagacc caaccccaaa cccctacccc agccccccag     1320

catccccaac agacatggcc ccctgtgaac gaaggacccc ccaaaccccc caccgagctg     1380

gagcctgagc cggagataga ggggctgctg acaccagtgc tggaggctgg cgatgtggat     1440

gaaggccctg tagcaaggcc tctcagcccc acgaggctgc agccagcact gccaccggag     1500

gcacagtcgg tgcccgagct ggaggaggtg gcacgggtgt tggcggaaat tccccggccc     1560

ctcaaacgca ggggctccat ggagcaggcc cctgctgtgg ccctgccccc tacccacaag     1620

aaacagtacc agcagatcat cagccgcctc ttccatcgtc atggggggcc agggcccggg     1680

gggccggagc cagagctgtc ccccatcact gagggatctg aggccagggc agggccccct     1740

gctcctgccc caccagctcc cattccaccc ccggccccgt cccagagcag cccaccagag     1800

cagccgcaga gcatggagat gcgctctgtg ctgcggaagg cgggctcccc gcgcaaggcc     1860

cgccgcgcgc gcctcaaccc tctggtgctc ctcctggacg cggcgctgac cggggagctg     1920

gaggtggtgc agcaggcggt gaaggagatg aacgacccga gccagcccaa cgaggagggc     1980

atcactgcct tgcacaacgc catctgcggc gccaactact ctatcgtgga tttcctcatc     2040

accgcgggtg ccaatgtcaa ctcccccgac agccacggct ggacaccctt gcactgcgcg     2100

gcgtcgtgca acgacacagt catctgcatg gcgctggtgc agcacggcgc tgcaatcttc     2160

gccaccacgc tcagcgacgg cgccaccgcc ttcgagaagt gcgaccctta ccgcgagggt     2220

tatgctgact gcgccaccta cctggcagac gtcgagcaga gtatggggct gatgaacagc     2280

ggggcagtgt acgctctctg ggactacagc gccgagttcg gggacgagct gtccttccgc     2340

gagggcgagt cggtcaccgt gctgcggagg gacgggccgg aggagaccga ctggtggtgg     2400

gccgcgctgc acggccagga gggctacgtg ccgcggaact acttcgggct gttccccagg     2460

gtgaagcctc aaaggagtaa agtctag                                         2487


<210>  2
<211>  828
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Asp Ser Glu Ala Phe Gln Ser Ala Arg Asp Phe Leu Asp Met Asn 
1               5                   10                  15      


Phe Gln Ser Leu Ala Met Lys His Met Asp Leu Lys Gln Met Glu Leu 
            20                  25                  30          


Asp Thr Ala Ala Ala Lys Val Asp Glu Leu Thr Lys Gln Leu Glu Ser 
        35                  40                  45              


Leu Trp Ser Asp Ser Pro Ala Pro Pro Gly Pro Gln Ala Gly Pro Pro 
    50                  55                  60                  


Ser Arg Pro Pro Arg Tyr Ser Ser Ser Ser Ile Pro Glu Pro Phe Gly 
65                  70                  75                  80  


Ser Arg Gly Ser Pro Arg Lys Ala Ala Thr Asp Gly Ala Asp Thr Pro 
                85                  90                  95      


Phe Gly Arg Ser Glu Ser Ala Pro Thr Leu His Pro Tyr Ser Pro Leu 
            100                 105                 110         


Ser Pro Lys Gly Arg Pro Ser Ser Pro Arg Thr Pro Leu Tyr Leu Gln 
        115                 120                 125             


Pro Asp Ala Tyr Gly Ser Leu Asp Arg Ala Thr Ser Pro Arg Pro Arg 
    130                 135                 140                 


Ala Phe Asp Gly Ala Gly Ser Ser Leu Gly Arg Ala Pro Ser Pro Arg 
145                 150                 155                 160 


Pro Gly Pro Gly Pro Leu Arg Gln Gln Gly Pro Pro Thr Pro Phe Asp 
                165                 170                 175     


Phe Leu Gly Arg Ala Gly Ser Pro Arg Gly Ser Pro Leu Ala Glu Gly 
            180                 185                 190         


Pro Gln Ala Phe Phe Pro Glu Arg Gly Pro Ser Pro Arg Pro Pro Ala 
        195                 200                 205             


Thr Ala Tyr Asp Ala Pro Ala Ser Ala Phe Gly Ser Ser Leu Leu Gly 
    210                 215                 220                 


Ser Gly Gly Ser Ala Phe Ala Pro Pro Leu Arg Ala Gln Asp Asp Leu 
225                 230                 235                 240 


Thr Leu Arg Arg Arg Pro Pro Lys Ala Trp Asn Glu Ser Asp Leu Asp 
                245                 250                 255     


Val Ala Tyr Glu Lys Lys Pro Ser Gln Thr Ala Ser Tyr Glu Arg Leu 
            260                 265                 270         


Asp Val Phe Ala Arg Pro Ala Ser Pro Ser Leu Gln Leu Leu Pro Trp 
        275                 280                 285             


Arg Glu Ser Ser Leu Asp Gly Leu Gly Gly Thr Gly Lys Asp Asn Leu 
    290                 295                 300                 


Thr Ser Ala Thr Leu Pro Arg Asn Tyr Lys Val Ser Pro Leu Ala Ser 
305                 310                 315                 320 


Asp Arg Arg Ser Asp Ala Gly Ser Tyr Arg Arg Ser Leu Gly Ser Ala 
                325                 330                 335     


Gly Pro Ser Gly Thr Leu Pro Arg Ser Trp Gln Pro Val Ser Arg Ile 
            340                 345                 350         


Pro Met Pro Pro Ser Ser Pro Gln Pro Arg Gly Ala Pro Arg Gln Arg 
        355                 360                 365             


Pro Ile Pro Leu Ser Met Ile Phe Lys Leu Gln Asn Ala Phe Trp Glu 
    370                 375                 380                 


His Gly Ala Ser Arg Ala Met Leu Pro Gly Ser Pro Leu Phe Thr Arg 
385                 390                 395                 400 


Ala Pro Pro Pro Lys Leu Gln Pro Gln Pro Gln Pro Gln Pro Gln Pro 
                405                 410                 415     


Gln Ser Gln Pro Gln Pro Gln Leu Pro Pro Gln Pro Gln Thr Gln Pro 
            420                 425                 430         


Gln Thr Pro Thr Pro Ala Pro Gln His Pro Gln Gln Thr Trp Pro Pro 
        435                 440                 445             


Val Asn Glu Gly Pro Pro Lys Pro Pro Thr Glu Leu Glu Pro Glu Pro 
    450                 455                 460                 


Glu Ile Glu Gly Leu Leu Thr Pro Val Leu Glu Ala Gly Asp Val Asp 
465                 470                 475                 480 


Glu Gly Pro Val Ala Arg Pro Leu Ser Pro Thr Arg Leu Gln Pro Ala 
                485                 490                 495     


Leu Pro Pro Glu Ala Gln Ser Val Pro Glu Leu Glu Glu Val Ala Arg 
            500                 505                 510         


Val Leu Ala Glu Ile Pro Arg Pro Leu Lys Arg Arg Gly Ser Met Glu 
        515                 520                 525             


Gln Ala Pro Ala Val Ala Leu Pro Pro Thr His Lys Lys Gln Tyr Gln 
    530                 535                 540                 


Gln Ile Ile Ser Arg Leu Phe His Arg His Gly Gly Pro Gly Pro Gly 
545                 550                 555                 560 


Gly Pro Glu Pro Glu Leu Ser Pro Ile Thr Glu Gly Ser Glu Ala Arg 
                565                 570                 575     


Ala Gly Pro Pro Ala Pro Ala Pro Pro Ala Pro Ile Pro Pro Pro Ala 
            580                 585                 590         


Pro Ser Gln Ser Ser Pro Pro Glu Gln Pro Gln Ser Met Glu Met Arg 
        595                 600                 605             


Ser Val Leu Arg Lys Ala Gly Ser Pro Arg Lys Ala Arg Arg Ala Arg 
    610                 615                 620                 


Leu Asn Pro Leu Val Leu Leu Leu Asp Ala Ala Leu Thr Gly Glu Leu 
625                 630                 635                 640 


Glu Val Val Gln Gln Ala Val Lys Glu Met Asn Asp Pro Ser Gln Pro 
                645                 650                 655     


Asn Glu Glu Gly Ile Thr Ala Leu His Asn Ala Ile Cys Gly Ala Asn 
            660                 665                 670         


Tyr Ser Ile Val Asp Phe Leu Ile Thr Ala Gly Ala Asn Val Asn Ser 
        675                 680                 685             


Pro Asp Ser His Gly Trp Thr Pro Leu His Cys Ala Ala Ser Cys Asn 
    690                 695                 700                 


Asp Thr Val Ile Cys Met Ala Leu Val Gln His Gly Ala Ala Ile Phe 
705                 710                 715                 720 


Ala Thr Thr Leu Ser Asp Gly Ala Thr Ala Phe Glu Lys Cys Asp Pro 
                725                 730                 735     


Tyr Arg Glu Gly Tyr Ala Asp Cys Ala Thr Tyr Leu Ala Asp Val Glu 
            740                 745                 750         


Gln Ser Met Gly Leu Met Asn Ser Gly Ala Val Tyr Ala Leu Trp Asp 
        755                 760                 765             


Tyr Ser Ala Glu Phe Gly Asp Glu Leu Ser Phe Arg Glu Gly Glu Ser 
    770                 775                 780                 


Val Thr Val Leu Arg Arg Asp Gly Pro Glu Glu Thr Asp Trp Trp Trp 
785                 790                 795                 800 


Ala Ala Leu His Gly Gln Glu Gly Tyr Val Pro Arg Asn Tyr Phe Gly 
                805                 810                 815     


Leu Phe Pro Arg Val Lys Pro Gln Arg Ser Lys Val 
            820                 825             


<210>  3
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  3
cggggacact ttgcgttc                                                     18


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  4
ttctgacgca cacctattgc                                                   20


<210>  5
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  5
gctttccacg acggtgag                                                     18


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  6
caagggttca aagacccaaa                                                   20


<210>  7
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Xaa can be any naturally occurring amino acid

<400>  7

Gln Glu Thr Phe Ser Asp Leu Trp Lys Xaa Leu Pro 
1               5                   10          


<210>  8
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  8

Met Pro Arg Phe Met Asp Tyr Trp Glu Gly Leu Asn 
1               5                   10          


<210>  9
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  9

Phe Met Asp Tyr Trp Glu Gly Leu Asn 
1               5                   


<210>  10
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Xaa is alpha-aminoisobutyric acid

<220>
<221>  MOD_RES
<222>  (6)..(6)
<223>  Xaa is alpha-aminoisobutyric acid

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  Xaa is alpha-aminoisobutyric acid

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  Xaa is alpha-aminoisobutyric acid

<400>  10

Glu Thr Phe Xaa Asp Xaa Trp Lys Xaa Leu Xaa Glu 
1               5                   10          


<210>  11
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Xaa is alpha-aminoisobutyric acid

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  Xaa is 1-aminocyclopropanecarboxylic acid

<400>  11

Phe Met Xaa Tyr Trp Glu Xaa Leu Asn 
1               5                   


<210>  12
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Xaa is alpha-aminoisobutyric acid

<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Xaa is phosphonomethylphenylalanine

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  Xaa is 1-aminocyclopropanecarboxylic acid

<400>  12

Phe Met Xaa Xaa Trp Glu Xaa Leu Asn 
1               5                   


<210>  13
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Xaa is alpha-aminoisobutyric acid

<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Xaa is phosphonomethylphenylalanine

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Xaa is 6-chloro-tryptophan

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  Xaa is 1-aminocyclopropanecarboxylic acid

<400>  13

Phe Met Xaa Xaa Xaa Glu Xaa Leu Asn 
1               5                   


<210>  14
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  PHOSPHORYLATION

<400>  14

His Pro Tyr Ser Pro Leu Ser Pro Lys Gly Arg Pro Ser 
1               5                   10              


<210>  15
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  PHOSPHORYLATION

<400>  15

Pro Glu Pro Phe Gly Ser Arg Gly Ser Pro Arg Lys Ala Ala Thr 
1               5                   10                  15  


