                         SEQUENCE LISTING

<110>  Icahn School of Medicine at Mount Sinai
       Skobe, Mihaela
       Das, Suvendu
 
<120>  FUNCTION OF CHEMOKINE RECEPTOR CCR8 IN MELANOMA METASTASIS

<130>  0028/83810-A-PCT/JPW/REB

<150>  61/606,169
<151>  2012-03-02

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  hCCR8 primer

<400>  1
ccctgtgatg cggaacttat                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  hCCR8 primer

<400>  2
gatggccttg gtcttgttgt                                                   20


<210>  3
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  hCCL1 primer

<400>  3
ggaagatgtg gacagcaaga gc                                                22


<210>  4
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  hCCL1 primer

<400>  4
tgtagggctg gtagtttcgg                                                   20


<210>  5
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  MC148 primer

<400>  5
aaaagctagc accatgaggg gcggagacgt cttc                                   34


<210>  6
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  MC148 primer

<400>  6
aaaagggccc tcaatgatga tgatgatgat gcagagactc gcacccggac catat            55


<210>  7
<211>  355
<212>  PRT
<213>  Homo sapiens

<400>  7

Met Asp Tyr Thr Leu Asp Leu Ser Val Thr Thr Val Thr Asp Tyr Tyr 
1               5                   10                  15      


Tyr Pro Asp Ile Phe Ser Ser Pro Cys Asp Ala Glu Leu Ile Gln Thr 
            20                  25                  30          


Asn Gly Lys Leu Leu Leu Ala Val Phe Tyr Cys Leu Leu Phe Val Phe 
        35                  40                  45              


Ser Leu Leu Gly Asn Ser Leu Val Ile Leu Val Leu Val Val Cys Lys 
    50                  55                  60                  


Lys Leu Arg Ser Ile Thr Asp Val Tyr Leu Leu Asn Leu Ala Leu Ser 
65                  70                  75                  80  


Asp Leu Leu Phe Val Phe Ser Phe Pro Phe Gln Thr Tyr Tyr Leu Leu 
                85                  90                  95      


Asp Gln Trp Val Phe Gly Thr Val Met Cys Lys Val Val Ser Gly Phe 
            100                 105                 110         


Tyr Tyr Ile Gly Phe Tyr Ser Ser Met Phe Phe Ile Thr Leu Met Ser 
        115                 120                 125             


Val Asp Arg Tyr Leu Ala Val Val His Ala Val Tyr Ala Leu Lys Val 
    130                 135                 140                 


Arg Thr Ile Arg Met Gly Thr Thr Leu Cys Leu Ala Val Trp Leu Thr 
145                 150                 155                 160 


Ala Ile Met Ala Thr Ile Pro Leu Leu Val Phe Tyr Gln Val Ala Ser 
                165                 170                 175     


Glu Asp Gly Val Leu Gln Cys Tyr Ser Phe Tyr Asn Gln Gln Thr Leu 
            180                 185                 190         


Lys Trp Lys Ile Phe Thr Asn Phe Lys Met Asn Ile Leu Gly Leu Leu 
        195                 200                 205             


Ile Pro Phe Thr Ile Phe Met Phe Cys Tyr Ile Lys Ile Leu His Gln 
    210                 215                 220                 


Leu Lys Arg Cys Gln Asn His Asn Lys Thr Lys Ala Ile Arg Leu Val 
225                 230                 235                 240 


Leu Ile Val Val Ile Ala Ser Leu Leu Phe Trp Val Pro Phe Asn Val 
                245                 250                 255     


Val Leu Phe Leu Thr Ser Leu His Ser Met His Ile Leu Asp Gly Cys 
            260                 265                 270         


Ser Ile Ser Gln Gln Leu Thr Tyr Ala Thr His Val Thr Glu Ile Ile 
        275                 280                 285             


Ser Phe Thr His Cys Cys Val Asn Pro Val Ile Tyr Ala Phe Val Gly 
    290                 295                 300                 


Glu Lys Phe Lys Lys His Leu Ser Glu Ile Phe Gln Lys Ser Cys Ser 
305                 310                 315                 320 


Gln Ile Phe Asn Tyr Leu Gly Arg Gln Met Pro Arg Glu Ser Cys Glu 
                325                 330                 335     


Lys Ser Ser Ser Cys Gln Gln His Ser Ser Arg Ser Ser Ser Val Asp 
            340                 345                 350         


Tyr Ile Leu 
        355 


<210>  8
<211>  1139
<212>  DNA
<213>  Homo sapiens

<400>  8
ccgctgcctt gatggattat acacttgacc tcagtgtgac aacagtgacc gactactact       60

accctgatat cttctcaagc ccctgtgatg cggaacttat tcagacaaat ggcaagttgc      120

tccttgctgt cttttattgc ctcctgtttg tattcagtct tctgggaaac agcctggtca      180

tcctggtcct tgtggtctgc aagaagctga ggagcatcac agatgtatac ctcttgaacc      240

tggccctgtc tgacctgctt tttgtcttct ccttcccctt tcagacctac tatctgctgg      300

accagtgggt gtttgggact gtaatgtgca aagtggtgtc tggcttttat tacattggct      360

tctacagcag catgtttttc atcaccctca tgagtgtgga caggtacctg gctgttgtcc      420

atgccgtgta tgccctaaag gtgaggacga tcaggatggg cacaacgctg tgcctggcag      480

tatggctaac cgccattatg gctaccatcc cattgctagt gttttaccaa gtggcctctg      540

aagatggtgt tctacagtgt tattcatttt acaatcaaca gactttgaag tggaagatct      600

tcaccaactt caaaatgaac attttaggct tgttgatccc attcaccatc tttatgttct      660

gctacattaa aatcctgcac cagctgaaga ggtgtcaaaa ccacaacaag accaaggcca      720

tcaggttggt gctcattgtg gtcattgcat ctttactttt ctgggtccca ttcaacgtgg      780

ttcttttcct cacttccttg cacagtatgc acatcttgga tggatgtagc ataagccaac      840

agctgactta tgccacccat gtcacagaaa tcatttcctt tactcactgc tgtgtgaacc      900

ctgttatcta tgcttttgtt ggggagaagt tcaagaaaca cctctcagaa atatttcaga      960

aaagttgcag ccaaatcttc aactacctag gaagacaaat gcctagggag agctgtgaaa     1020

agtcatcatc ctgccagcag cactcctccc gttcctccag cgtagactac attttgtgag     1080

gatcaatgaa gactaaatat aaaaaacatt ttcttgaatg gcatgctagt agcagtgag      1139


<210>  9
<211>  96
<212>  PRT
<213>  Homo sapiens

<400>  9

Met Gln Ile Ile Thr Thr Ala Leu Val Cys Leu Leu Leu Ala Gly Met 
1               5                   10                  15      


Trp Pro Glu Asp Val Asp Ser Lys Ser Met Gln Val Pro Phe Ser Arg 
            20                  25                  30          


Cys Cys Phe Ser Phe Ala Glu Gln Glu Ile Pro Leu Arg Ala Ile Leu 
        35                  40                  45              


Cys Tyr Arg Asn Thr Ser Ser Ile Cys Ser Asn Glu Gly Leu Ile Phe 
    50                  55                  60                  


Lys Leu Lys Arg Gly Lys Glu Ala Cys Ala Leu Asp Thr Val Gly Trp 
65                  70                  75                  80  


Val Gln Arg His Arg Lys Met Leu Arg His Cys Pro Ser Lys Arg Lys 
                85                  90                  95      


<210>  10
<211>  349
<212>  DNA
<213>  Homo sapiens

<400>  10
gtacaaaaaa gcagaagggc cgtcaaggcc caccatgcag atcatcacca cagccctggt       60

gtgcttgctg ctagctggga tgtggccgga agatgtggac agcaagagca tgcaggtacc      120

cttctccaga tgttgcttct catttgcgga gcaagagatt cccctgaggg caatcctgtg      180

ttacagaaat accagctcca tctgctccaa tgagggctta atattcaagc tgaagagagg      240

caaagaggcc tgcgccttgg acacagttgg atgggttcag aggcacagaa aaatgctgag      300

gcactgcccg tcaaaaagaa aaggcctcat gggcccagct ttcttgtac                  349


<210>  11
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  shCCR8(1) shRNA sequence (the DNA sequence that encodes the 
       portion of the shRNA that targets (i.e., is complementary to) 
       CCR8 transcripts)

<400>  11
ggattataca cttgacctca gtg                                               23


<210>  12
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  shCCR8(2) shRNA sequence (the DNA sequence that encodes the 
       portion of the shRNA that targets (i.e., is complementary to) 
       CCR8 transcripts)

<400>  12
cctccagcgt agactacatt t                                                 21


<210>  13
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  shScrambled shRNA sequence (the DNA sequence that encodes the 
       scrambled shRNA control sequence)

<400>  13
cctaaggtta agtcgccctc gc                                                22


