                         SEQUENCE LISTING

<110>  CSIR, IN
 
<120>  PEPTIDE INHIBITORS AS NOVEL ANTI-HIV THERAPEUTICS

<130>  0594DEL2012

<160>  71    

<170>  PatentIn version 3.5

<210>  1
<211>  6234
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nefwt sequence cloned in VP16pCDNA+pACT vector

<400>  1
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgac gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa      540

tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa      600

tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactgcga      660

tcgcccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc      720

agagctcgtt tagtgaaccg tcagatcact agaagcttta ttgcggtagt ttatcacagt      780

taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag ctgcagtgac      840

tctcttaagg tagccttgca gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt      900

tacaagacag gtttaaggag accaatagaa actgggcttg tcgagacaga gaagactctt      960

gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagg     1020

tgtccactcc cagttcaatt acagctctta aggctagagt acttaatacg actcactata     1080

ggctagccag cttgaagcaa gcctcctgaa agatgaagct actgtcttct atcgaacaag     1140

catgcccaaa aaagaagaga aaggtagatg aattcccggg gatctcgacg gcccccccga     1200

ccgatgtcag cctgggggac gagctccact tagacggcga ggacgtggcg atggcgcatg     1260

ccgacgcgct agacgatttc gatctggaca tgttggggga cggggattcc ccgggtccgg     1320

gatcgccagg gatccgtgga gcacttacaa gcagcaacac agcccaaact aatgctgatt     1380

gtgcctggct ggaagcacaa gaggaggaag aggaagtagg gtttccagtc agacctcagg     1440

tgccgctcag accaatgact tataaggggg cagtagatct cagcttcttt ttaaaagaaa     1500

aggggggact ggaagggtta acttactcta agaaaagaca agaaatcctt gatttgtggg     1560

tctatcacac acaaggctac ttccctgatt ggcaatgcta cacaccggga ccaggggtca     1620

gatacccact gacttttgga tggtgcttca agctagtgcc agttgaccca gatgaagtag     1680

gagaagccaa caatggagag aataactgtt tgctacaccc tataagcctg catggaatgg     1740

aggatccgtc gacttgacgc gttgatatca tctagagggc cctattctat agtgtcacct     1800

aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt     1860

ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta     1920

ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg     1980

ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc     2040

ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca     2100

cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc     2160

tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac     2220

gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag     2280

tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc     2340

atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg     2400

actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata     2460

agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa     2520

cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca     2580

ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc     2640

cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat     2700

agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc     2760

gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga     2820

gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc     2880

gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat     2940

gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg     3000

ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc     3060

gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca     3120

ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct     3180

cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga     3240

tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg     3300

gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat     3360

cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga     3420

gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg     3480

cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg     3540

ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat     3600

agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct     3660

cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga     3720

cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg     3780

ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt     3840

ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc     3900

caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat     3960

ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat     4020

gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca     4080

tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga     4140

agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg     4200

cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc     4260

caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac     4320

tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata     4380

cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa     4440

aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct     4500

gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa     4560

agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg     4620

cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca     4680

cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa     4740

ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg     4800

gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg     4860

tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg     4920

acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc     4980

tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag     5040

attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac     5100

gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc     5160

ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag     5220

taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt     5280

ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag     5340

ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca     5400

gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact     5460

ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca     5520

gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg     5580

tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc     5640

atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg     5700

gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca     5760

tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt     5820

atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc     5880

agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc     5940

ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca     6000

tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa     6060

aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat     6120

tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa     6180

aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc           6234


<210>  2
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nef RP01

<400>  2

Met Lys Leu Leu Ser Ser Ile Glu Gln Ala Cys Pro Lys Lys Lys Arg 
1               5                   10                  15      


Lys Val Asp Glu Phe Pro Gly Ile Ser Thr Ala Pro Pro Thr Asp Val 
            20                  25                  30          


Ser Leu Gly Asp Glu Leu His Leu Asp Gly Glu Asp Val Ala Met Ala 
        35                  40                  45              


His Ala Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Asp Gly 
    50                  55                  60                  


Asp Ser Pro Gly Pro Gly Ser Pro Gly Ile Arg Gly Ala Leu Thr Ser 
65                  70                  75                  80  


Ser Asn Thr Ala Gln Thr Asn Ala Asp Cys Ala Trp Leu Glu Ala Gln 
                85                  90                  95      


Glu Glu Glu Glu Glu Val Gly Phe Pro Val Arg Pro Gln Val Pro Leu 
            100                 105                 110         


Arg Pro Met Thr Tyr Lys Gly Ala Val Asp Leu Ser Phe Phe Leu Lys 
        115                 120                 125             


Glu Lys Gly Gly Leu Glu Gly Leu Thr Tyr Ser Lys Lys Arg Gln Glu 
    130                 135                 140                 


Ile Leu Asp Leu Trp Val Tyr His Thr Gln Gly Tyr Phe Pro Asp Trp 
145                 150                 155                 160 


Gln Cys Tyr Thr Pro Gly Pro Gly Val Arg Tyr Pro Leu Thr Phe Gly 
                165                 170                 175     


Trp Cys Phe Lys Leu Val Pro Val Asp Pro Asp Glu Val Gly Glu Ala 
            180                 185                 190         


Asn Asn Gly Glu Asn Asn Cys Leu Leu His Pro Ile Ser Leu His Gly 
        195                 200                 205             


Met Glu Asp Pro Ser Thr 
    210                 


<210>  3
<211>  4955
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reporter construct pG5Luc

<400>  3
ggtaccgagt ttctagacgg agtactgtcc tccgagcgga gtactgtcct ccgactcgag       60

cggagtactg tcctccgatc ggagtactgt cctccgcgaa ttccggagta ctgtcctccg      120

aagacgctag cggggggcta taaaaggggg tgggggcgtt cgtcctcact ctagatctgc      180

gatctaagta agcttggcat tccggtactg ttggtaaagc caccatggaa gacgccaaaa      240

acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac      300

tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac      360

atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag      420

ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc      480

ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga      540

acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg      600

tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca      660

tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca      720

cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct      780

tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta      840

aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta      900

tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg      960

gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt     1020

atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt caaagtgcgc     1080

tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac aaatacgatt     1140

tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa gtcggggaag     1200

cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc actgagacta     1260

catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg     1320

ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc     1380

aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta aacaatccgg     1440

aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact     1500

gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt aagtacaaag     1560

gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc aacatcttcg     1620

acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg     1680

ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag     1740

taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc     1800

ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg     1860

gaaagatcgc cgtgtaattc tagagtcggg gcggccggcc gcttcgagca gacatgataa     1920

gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt     1980

gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta     2040

acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt     2100

aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgtcg accgatgccc     2160

ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc     2220

gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc     2280

cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc     2340

tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat     2400

gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt     2460

ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg     2520

aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc     2580

tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt     2640

ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa     2700

gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta     2760

tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa     2820

caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa     2880

ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt     2940

cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt     3000

ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat     3060

cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat     3120

gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc     3180

aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc     3240

acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta     3300

gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga     3360

cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg     3420

cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc     3480

tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat     3540

cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag     3600

gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat     3660

cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa     3720

ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa     3780

gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga     3840

taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg     3900

gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc     3960

acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg     4020

aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact     4080

cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat     4140

atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt     4200

gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag     4260

cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt     4320

tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt     4380

ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg     4440

tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt     4500

taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt     4560

tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca     4620

aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcccatt cgccattcag     4680

gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagcccaa     4740

gctaccatga taagtaagta atattaaggt acgggaggta cttggagcgg ccgcaataaa     4800

atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca     4860

tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt     4920

gcaagtgcag gtgccagaac atttctctat cgata                                4955


<210>  4
<211>  7368
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct sequence containing ASK1(a) cloned in pBIND

<400>  4
tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta       60

ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc      120

aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg      180

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      240

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      300

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      360

ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga      420

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg      480

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac      540

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt      600

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg      660

cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata      720

agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac      780

agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt      840

gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa      900

ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact      960

cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac     1020

aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact     1080

ataggctagc cagcttgaag caagcctcct gaaagatgaa gctactgtct tctatcgaac     1140

aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg     1200

ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc     1260

tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc     1320

tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata     1380

taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag     1440

atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg     1500

cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgccgg     1560

aattcccggg gatccgtacg gaggcggacg agggcatcac tttctctgtg ccacccttcg     1620

ccccctcggg cttctgcacc atccccgagg gcggcatctg caggagggga ggagcggcgg     1680

cggtgggcga gggcgaggag caccagctgc caccgccgcc gccgggcagt ttctggaacg     1740

tggagagcgc cgctgcccct ggcatcggtt gtccggcggc cacctcctcg agcagtgcca     1800

cccgaggccg gggcagctct gttggcgggg gcagccgacg gaccacggtg gcatatgtga     1860

tcaacgaagc gagccaaggg caactggtgg tggccgagag cgaggccctg cagagcttgc     1920

gggaggcgtg cgagacagtg ggcgccaccc tggaaaccct gcattttggg aaactcgact     1980

ttggagaaac caccgtgctg gaccgctttt acaatgcaga tattgcggtg gtggagatga     2040

gcgatgcctt ccggcagccg tccttgtttt accaccttgg ggtgagagaa agtttcagca     2100

tggccaacaa catcatcctc tactgtgata ctaactcgga ctctctgcag tcactgaagg     2160

aaatcatttg ccagaagaat actatgtgca ctgggaacta cacctttgtt ccttacatga     2220

taactccaca taacaaagtc tactgctgtg acagcagctt catgaagggg ttgacagagc     2280

tcatgcaacc gaacttcgag ctgcttcttg gacccatctg cttacctctt gtggatcgtt     2340

ttattcaact tttgaaggtg gcacaagcaa gttctagcca gtacttccgg gaatctatac     2400

tcaatgacat caggaaagct cgtaatttat acactggtaa agaattggca gctgagttgg     2460

caagaattcg gcagcgagta gataatatcg aagtcttgac agcagatatt gtcataaatc     2520

tgttactttc ctacagagat atccaggact atgattctat tgtgaagctg gtagagactt     2580

tagaaaaact gccaaccttt gatttgtcta gagcggccgc aggtacctga ataactaagg     2640

ccgcttccct ttagtgaggg ttaatgcttc gagcagacat gataagatac attgatgagt     2700

ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg     2760

ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca     2820

ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaagc aagtaaaacc     2880

tctacaaatg tggtaaaatc cgataaggat cgatccgggc tggcgtaata gcgaagaggc     2940

ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt     3000

agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc     3060

agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttcacgccac gttcgccggc     3120

tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg     3180

cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga     3240

tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc     3300

caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg     3360

ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt     3420

aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg     3480

gtatttcaca ccgcatacgc ggatcttccg taccttctga ggcggaaaga accagctgtg     3540

gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca     3600

aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg     3660

cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc     3720

gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat     3780

tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg     3840

aggaggcttt tttggaggcc taggcttttg caaaaagctt gattcttctg acacaacagt     3900

ctcgaactta aggctagaat tctggctaca ggtaagcgcc cctaaaatcc ctttgggcac     3960

aatgtgtcct gaggggagag gcagcgacct gtagatggga cgggggcact aaccctcagg     4020

tttggggctt ctgaatgtga gtatcgccat gtaagcccag tatttggcca atctcagaaa     4080

gctcctggtc cctggaggga tggagagaga aaaacaaaca gctcctggag cagggagagt     4140

gctggcctct tgctctccgg ctccctctgt tgccctctgg tttctcccca ggctcccgga     4200

cgtcctctag ccaccatgac ttcgaaagtt tatgatccag aacaaaggaa acggatgata     4260

actggtccgc agtggtgggc cagatgtaaa caaatgaatg ttcttgattc atttattaat     4320

tattatgatt cagaaaaaca tgcagaaaat gctgttattt ttttacatgg taacgcggcc     4380

tcttcttatt tatggcgaca tgttgtgcca catattgagc cagtagcgcg gtgtattata     4440

ccagacctta ttggtatggg caaatcaggc aaatctggta atggttctta taggttactt     4500

gatcattaca aatatcttac tgcatggttt gaacttctta atttaccaaa gaagatcatt     4560

tttgtcggcc atgattgggg tgcttgtttg gcatttcatt atagctatga gcatcaagat     4620

aagatcaaag caatagttca cgctgaaagt gtagtagatg tgattgaatc atgggatgaa     4680

tggcctgata ttgaagaaga tattgcgttg atcaaatctg aagaaggaga aaaaatggtt     4740

ttggagaata acttcttcgt ggaaaccatg ttgccatcaa aaatcatgag aaagttagaa     4800

ccagaagaat ttgcagcata tcttgaacca ttcaaagaga aaggtgaagt tcgtcgtcca     4860

acattatcat ggcctcgtga aatcccgtta gtaaaaggtg gtaaacctga cgttgtacaa     4920

attgttagga attataatgc ttatctacgt gcaagtgatg atttaccaaa aatgtttatt     4980

gaatcggacc caggattctt ttccaatgct attgttgaag gtgccaagaa gtttcctaat     5040

actgaatttg tcaaagtaaa aggtcttcat ttttcgcaag aagatgcacc tgatgaaatg     5100

ggaaaatata tcaaatcgtt cgttgagcga gttctcaaaa atgaacaata attctagccc     5160

tgaataagtg ataataagcg gatgaatggc agaaattcgt cgaagcgcaa taaaatatct     5220

ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagcgat aaggatcgga     5280

agatccgcgt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc     5340

cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg     5400

cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat     5460

caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca     5520

tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc     5580

ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct     5640

gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg     5700

cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg     5760

tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc     5820

tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca     5880

cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac     5940

tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa     6000

agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg     6060

ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt     6120

ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg     6180

aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc     6240

gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga     6300

tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta     6360

ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc     6420

cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg     6480

atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt     6540

cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa     6600

ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt     6660

cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt     6720

ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt     6780

tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga     6840

taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag     6900

caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata     6960

agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg     7020

gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga     7080

gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca     7140

ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa     7200

acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt     7260

tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac     7320

ggttcctggc cttttgctgg ccttttgctc acatggctcg acagatct                  7368


<210>  5
<211>  499
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gal4 ASK1 (a) fusion protein sequence

<400>  5

Met Lys Leu Leu Ser Ser Ile Glu Gln Ala Cys Asp Ile Cys Arg Leu 
1               5                   10                  15      


Lys Lys Leu Lys Cys Ser Lys Glu Lys Pro Lys Cys Ala Lys Cys Leu 
            20                  25                  30          


Lys Asn Asn Trp Glu Cys Arg Tyr Ser Pro Lys Thr Lys Arg Ser Pro 
        35                  40                  45              


Leu Thr Arg Ala His Leu Thr Glu Val Glu Ser Arg Leu Glu Arg Leu 
    50                  55                  60                  


Glu Gln Leu Phe Leu Leu Ile Phe Pro Arg Glu Asp Leu Asp Met Ile 
65                  70                  75                  80  


Leu Lys Met Asp Ser Leu Gln Asp Ile Lys Ala Leu Leu Thr Gly Leu 
                85                  90                  95      


Phe Val Gln Asp Asn Val Asn Lys Asp Ala Val Thr Asp Arg Leu Ala 
            100                 105                 110         


Ser Val Glu Thr Asp Met Pro Leu Thr Leu Arg Gln His Arg Ile Ser 
        115                 120                 125             


Ala Thr Ser Ser Ser Glu Glu Ser Ser Asn Lys Gly Gln Arg Gln Leu 
    130                 135                 140                 


Thr Val Ser Pro Glu Phe Pro Gly Ile Arg Thr Glu Ala Asp Glu Gly 
145                 150                 155                 160 


Ile Thr Phe Ser Val Pro Pro Phe Ala Pro Ser Gly Phe Cys Thr Ile 
                165                 170                 175     


Pro Glu Gly Gly Ile Cys Arg Arg Gly Gly Ala Ala Ala Val Gly Glu 
            180                 185                 190         


Gly Glu Glu His Gln Leu Pro Pro Pro Pro Pro Gly Ser Phe Trp Asn 
        195                 200                 205             


Val Glu Ser Ala Ala Ala Pro Gly Ile Gly Cys Pro Ala Ala Thr Ser 
    210                 215                 220                 


Ser Ser Ser Ala Thr Arg Gly Arg Gly Ser Ser Val Gly Gly Gly Ser 
225                 230                 235                 240 


Arg Arg Thr Thr Val Ala Tyr Val Ile Asn Glu Ala Ser Gln Gly Gln 
                245                 250                 255     


Leu Val Val Ala Glu Ser Glu Ala Leu Gln Ser Leu Arg Glu Ala Cys 
            260                 265                 270         


Glu Thr Val Gly Ala Thr Leu Glu Thr Leu His Phe Gly Lys Leu Asp 
        275                 280                 285             


Phe Gly Glu Thr Thr Val Leu Asp Arg Phe Tyr Asn Ala Asp Ile Ala 
    290                 295                 300                 


Val Val Glu Met Ser Asp Ala Phe Arg Gln Pro Ser Leu Phe Tyr His 
305                 310                 315                 320 


Leu Gly Val Arg Glu Ser Phe Ser Met Ala Asn Asn Ile Ile Leu Tyr 
                325                 330                 335     


Cys Asp Thr Asn Ser Asp Ser Leu Gln Ser Leu Lys Glu Ile Ile Cys 
            340                 345                 350         


Gln Lys Asn Thr Met Cys Thr Gly Asn Tyr Thr Phe Val Pro Tyr Met 
        355                 360                 365             


Ile Thr Pro His Asn Lys Val Tyr Cys Cys Asp Ser Ser Phe Met Lys 
    370                 375                 380                 


Gly Leu Thr Glu Leu Met Gln Pro Asn Phe Glu Leu Leu Leu Gly Pro 
385                 390                 395                 400 


Ile Cys Leu Pro Leu Val Asp Arg Phe Ile Gln Leu Leu Lys Val Ala 
                405                 410                 415     


Gln Ala Ser Ser Ser Gln Tyr Phe Arg Glu Ser Ile Leu Asn Asp Ile 
            420                 425                 430         


Arg Lys Ala Arg Asn Leu Tyr Thr Gly Lys Glu Leu Ala Ala Glu Leu 
        435                 440                 445             


Ala Arg Ile Arg Gln Arg Val Asp Asn Ile Glu Val Leu Thr Ala Asp 
    450                 455                 460                 


Ile Val Ile Asn Leu Leu Leu Ser Tyr Arg Asp Ile Gln Asp Tyr Asp 
465                 470                 475                 480 


Ser Ile Val Lys Leu Val Glu Thr Leu Glu Lys Leu Pro Thr Phe Asp 
                485                 490                 495     


Leu Ser Arg 
            


<210>  6
<211>  7368
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct sequence containing ASK1(b) cloned in pBIND

<400>  6
tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta       60

ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc      120

aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg      180

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      240

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      300

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      360

ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga      420

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg      480

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac      540

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt      600

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg      660

cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata      720

agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac      780

agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt      840

gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa      900

ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact      960

cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac     1020

aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact     1080

ataggctagc cagcttgaag caagcctcct gaaagatgaa gctactgtct tctatcgaac     1140

aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg     1200

ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc     1260

tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc     1320

tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata     1380

taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag     1440

atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg     1500

cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgccgg     1560

aattcccggg gatccgtacg gaggcggacg agggcatcac tttctctgtg ccacccttcg     1620

ccccctcggg cttctgcacc atccccgagg gcggcatctg caggagggga ggagcggcgg     1680

cggtgggcga gggcgaggag caccagctgc caccgccgcc gccgggcagt ttctggaacg     1740

tggagagcgc cgctgcccct ggcatcggtt gtccggcggc cacctcctcg agcagtgcca     1800

cccgaggccg gggcagctct gttggcgggg gcagccgacg gaccacggtg gcatatgtga     1860

tcaacgaagc gagccaaggg caactggtgg tggccgagag cgaggccctg cagagcttgc     1920

gggaggcgtg cgagacagtg ggcgccaccc tggaaaccct gcattttggg aaactcgact     1980

ttggagaaac caccgtgctg gaccgctttt acaatgcaga tattgcggtg gtggagatga     2040

gcgatgcctt ccggcagccg tccttgtttt accaccttgg ggtgagagaa agtttcagca     2100

tggccaacaa catcatcctc tactgtgata ctaactcgga ctctctgcag tcactgaagg     2160

aaatcatttg ccagaagaat actatgtgca ctgggaacta cacctttgtt ccttacatga     2220

taactccaca taacaaagtc tactgctgtg acagcagctt catgaagggg ttgacagagc     2280

tcatgcaacc gaacttcgag ctgcttcttg gacccatctg cttacctctt gtggatcgtt     2340

ttattcaact tttgaaggtg gcacaagcaa gttctagcca gtacttccgg gaatctatac     2400

tcaatgacat caggaaagct cgtaatttat acactggtaa agaattggca gctgagttgg     2460

caagaattcg gcagcgagta gataatatcg aagtcttgac agcagatatt gtcataaatc     2520

tgttactttc ctacagagat atccaggact atgattctat tgtgaagctg gtagagactt     2580

tagaaaaact gccaaccttt gatttgtcta gagcggccgc aggtacctga ataactaagg     2640

ccgcttccct ttagtgaggg ttaatgcttc gagcagacat gataagatac attgatgagt     2700

ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg     2760

ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca     2820

ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaagc aagtaaaacc     2880

tctacaaatg tggtaaaatc cgataaggat cgatccgggc tggcgtaata gcgaagaggc     2940

ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt     3000

agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc     3060

agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttcacgccac gttcgccggc     3120

tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg     3180

cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga     3240

tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc     3300

caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg     3360

ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt     3420

aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg     3480

gtatttcaca ccgcatacgc ggatcttccg taccttctga ggcggaaaga accagctgtg     3540

gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca     3600

aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg     3660

cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc     3720

gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat     3780

tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg     3840

aggaggcttt tttggaggcc taggcttttg caaaaagctt gattcttctg acacaacagt     3900

ctcgaactta aggctagaat tctggctaca ggtaagcgcc cctaaaatcc ctttgggcac     3960

aatgtgtcct gaggggagag gcagcgacct gtagatggga cgggggcact aaccctcagg     4020

tttggggctt ctgaatgtga gtatcgccat gtaagcccag tatttggcca atctcagaaa     4080

gctcctggtc cctggaggga tggagagaga aaaacaaaca gctcctggag cagggagagt     4140

gctggcctct tgctctccgg ctccctctgt tgccctctgg tttctcccca ggctcccgga     4200

cgtcctctag ccaccatgac ttcgaaagtt tatgatccag aacaaaggaa acggatgata     4260

actggtccgc agtggtgggc cagatgtaaa caaatgaatg ttcttgattc atttattaat     4320

tattatgatt cagaaaaaca tgcagaaaat gctgttattt ttttacatgg taacgcggcc     4380

tcttcttatt tatggcgaca tgttgtgcca catattgagc cagtagcgcg gtgtattata     4440

ccagacctta ttggtatggg caaatcaggc aaatctggta atggttctta taggttactt     4500

gatcattaca aatatcttac tgcatggttt gaacttctta atttaccaaa gaagatcatt     4560

tttgtcggcc atgattgggg tgcttgtttg gcatttcatt atagctatga gcatcaagat     4620

aagatcaaag caatagttca cgctgaaagt gtagtagatg tgattgaatc atgggatgaa     4680

tggcctgata ttgaagaaga tattgcgttg atcaaatctg aagaaggaga aaaaatggtt     4740

ttggagaata acttcttcgt ggaaaccatg ttgccatcaa aaatcatgag aaagttagaa     4800

ccagaagaat ttgcagcata tcttgaacca ttcaaagaga aaggtgaagt tcgtcgtcca     4860

acattatcat ggcctcgtga aatcccgtta gtaaaaggtg gtaaacctga cgttgtacaa     4920

attgttagga attataatgc ttatctacgt gcaagtgatg atttaccaaa aatgtttatt     4980

gaatcggacc caggattctt ttccaatgct attgttgaag gtgccaagaa gtttcctaat     5040

actgaatttg tcaaagtaaa aggtcttcat ttttcgcaag aagatgcacc tgatgaaatg     5100

ggaaaatata tcaaatcgtt cgttgagcga gttctcaaaa atgaacaata attctagccc     5160

tgaataagtg ataataagcg gatgaatggc agaaattcgt cgaagcgcaa taaaatatct     5220

ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagcgat aaggatcgga     5280

agatccgcgt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc     5340

cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg     5400

cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat     5460

caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca     5520

tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc     5580

ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct     5640

gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg     5700

cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg     5760

tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc     5820

tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca     5880

cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac     5940

tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa     6000

agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg     6060

ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt     6120

ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg     6180

aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc     6240

gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga     6300

tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta     6360

ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc     6420

cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg     6480

atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt     6540

cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa     6600

ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt     6660

cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt     6720

ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt     6780

tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga     6840

taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag     6900

caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata     6960

agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg     7020

gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga     7080

gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca     7140

ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa     7200

acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt     7260

tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac     7320

ggttcctggc cttttgctgg ccttttgctc acatggctcg acagatct                  7368


<210>  7
<211>  505
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gal4 ASK1(b) fusion protein sequence

<400>  7

Met Lys Leu Leu Ser Ser Ile Glu Gln Ala Cys Asp Ile Cys Arg Leu 
1               5                   10                  15      


Lys Lys Leu Lys Cys Ser Lys Glu Lys Pro Lys Cys Ala Lys Cys Leu 
            20                  25                  30          


Lys Asn Asn Trp Glu Cys Arg Tyr Ser Pro Lys Thr Lys Arg Ser Pro 
        35                  40                  45              


Leu Thr Arg Ala His Leu Thr Glu Val Glu Ser Arg Leu Glu Arg Leu 
    50                  55                  60                  


Glu Gln Leu Phe Leu Leu Ile Phe Pro Arg Glu Asp Leu Asp Met Ile 
65                  70                  75                  80  


Leu Lys Met Asp Ser Leu Gln Asp Ile Lys Ala Leu Leu Thr Gly Leu 
                85                  90                  95      


Phe Val Gln Asp Asn Val Asn Lys Asp Ala Val Thr Asp Arg Leu Ala 
            100                 105                 110         


Ser Val Glu Thr Asp Met Pro Leu Thr Leu Arg Gln His Arg Ile Ser 
        115                 120                 125             


Ala Thr Ser Ser Ser Glu Glu Ser Ser Asn Lys Gly Gln Arg Gln Leu 
    130                 135                 140                 


Thr Val Ser Pro Glu Phe Pro Gly Ile Arg Ser Tyr Arg Asp Ile Gln 
145                 150                 155                 160 


Asp Tyr Asp Ser Ile Val Lys Leu Val Glu Thr Leu Glu Lys Leu Pro 
                165                 170                 175     


Thr Phe Asp Leu Ala Ser His His His Val Lys Phe His Tyr Ala Phe 
            180                 185                 190         


Ala Leu Asn Arg Arg Asn Leu Pro Gly Asp Arg Ala Lys Ala Leu Asp 
        195                 200                 205             


Ile Met Ile Pro Met Val Gln Ser Glu Gly Gln Val Ala Ser Asp Met 
    210                 215                 220                 


Tyr Cys Leu Val Gly Arg Ile Tyr Lys Asp Met Phe Leu Asp Ser Asn 
225                 230                 235                 240 


Phe Thr Asp Thr Glu Ser Arg Asp His Gly Ala Ser Trp Phe Lys Lys 
                245                 250                 255     


Ala Phe Glu Ser Glu Pro Thr Leu Gln Ser Gly Ile Asn Tyr Ala Val 
            260                 265                 270         


Leu Leu Leu Ala Ala Gly His Gln Phe Glu Ser Ser Phe Glu Leu Arg 
        275                 280                 285             


Lys Val Gly Val Lys Leu Ser Ser Leu Leu Gly Lys Lys Gly Asn Leu 
    290                 295                 300                 


Glu Lys Leu Gln Ser Tyr Trp Glu Val Gly Phe Phe Leu Gly Ala Ser 
305                 310                 315                 320 


Val Leu Ala Asn Asp His Met Arg Val Ile Gln Ala Ser Glu Lys Leu 
                325                 330                 335     


Phe Lys Leu Lys Thr Pro Ala Trp Tyr Leu Lys Ser Ile Val Glu Thr 
            340                 345                 350         


Ile Leu Ile Tyr Lys His Phe Val Lys Leu Thr Thr Glu Gln Pro Val 
        355                 360                 365             


Ala Lys Gln Glu Leu Val Asp Phe Trp Met Asp Phe Leu Val Glu Ala 
    370                 375                 380                 


Thr Lys Thr Asp Val Thr Val Val Arg Phe Pro Val Leu Ile Leu Glu 
385                 390                 395                 400 


Pro Thr Lys Ile Tyr Gln Pro Ser Tyr Leu Ser Ile Asn Asn Glu Val 
                405                 410                 415     


Glu Glu Lys Thr Ile Ser Ile Trp His Val Leu Pro Asp Asp Lys Lys 
            420                 425                 430         


Gly Ile His Glu Trp Asn Phe Ser Ala Ser Ser Val Arg Gly Val Ser 
        435                 440                 445             


Ile Ser Lys Phe Glu Glu Arg Cys Cys Phe Leu Tyr Val Leu His Asn 
    450                 455                 460                 


Ser Asp Asp Phe Gln Ile Tyr Phe Cys Thr Glu Leu His Cys Lys Lys 
465                 470                 475                 480 


Phe Phe Glu Met Val Asn Thr Ile Thr Glu Glu Lys Gly Arg Ser Thr 
                485                 490                 495     


Glu Glu Gly Asp Cys Glu Ser Asp Leu 
            500                 505 


<210>  8
<211>  7239
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct sequence containing ASK1(c) cloned in pBIND

<400>  8
tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta       60

ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc      120

aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg      180

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      240

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      300

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      360

ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga      420

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg      480

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac      540

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt      600

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg      660

cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata      720

agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac      780

agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt      840

gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa      900

ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact      960

cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac     1020

aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact     1080

ataggctagc cagcttgaag caagcctcct gaaagatgaa gctactgtct tctatcgaac     1140

aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg     1200

ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc     1260

tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc     1320

tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata     1380

taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag     1440

atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg     1500

cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgccgg     1560

aattcccggg gatccgttct tctgtcaggg gagtgagtat ttctaaattt gaagaaagat     1620

gctgctttct ttatgtgctt cacaattctg atgatttcca aatctatttc tgtacagaac     1680

ttcattgtaa aaagtttttt gagatggtga acaccattac cgaagagaag gggagaagca     1740

cagaggaagg agactgtgaa agtgacttgc tggagtatga ctatgaatat gatgaaaatg     1800

gtgacagagt cgttttagga aaaggcactt atgggatagt ctacgcaggt cgggacttga     1860

gcaaccaagt cagaattgct attaaggaaa tcccagagag agacagcaga tactctcagc     1920

ccctgcatga agaaatagca ttgcataaac acctgaagca caaaaatatt gtccagtatc     1980

tgggctcttt cagtgagaat ggtttcatta aaatcttcat ggagcaggtc cctggaggaa     2040

gtctttctgc tctccttcgt tccaaatggg gtccattaaa agacaatgag caaacaattg     2100

gcttttatac aaagcaaata ctggaaggat taaaatatct ccatgacaat cagatagttc     2160

accgggacat aaagggtgac aatgtgttga ttaataccta cagtggtgtt ctcaagatct     2220

ctgacttcgg aacatcaaag aggcttgctg gcataaaccc ctgtactgaa acttttactg     2280

gtaccctcca gtatatggca ccagaaataa tagataaagg accaagaggc tacggaaaag     2340

cagcagacat ctggtctctg ggctgtacaa tcattgaaat ggccacagga aaacccccat     2400

tttatgaact gggagaacca caagcagcta tgttcaaggt gggaatgttt aaagtccacc     2460

ctgagatccc atctagatct agagcggccg caggtacctg aataactaag gccgcttccc     2520

tttagtgagg gttaatgctt cgagcagaca tgataagata cattgatgag tttggacaaa     2580

ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt     2640

tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta     2700

tgtttcaggt tcagggggag atgtgggagg ttttttaaag caagtaaaac ctctacaaat     2760

gtggtaaaat ccgataagga tcgatccggg ctggcgtaat agcgaagagg cccgcaccga     2820

tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca     2880

ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta     2940

gcgcccgctc ctttcgcttt cttcccttcc tttcacgcca cgttcgccgg ctttccccgt     3000

caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac     3060

cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt     3120

tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga     3180

acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg     3240

gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata     3300

ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac     3360

accgcatacg cggatcttcc gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg     3420

tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca     3480

tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat     3540

gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc     3600

gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat     3660

ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt     3720

ttttggaggc ctaggctttt gcaaaaagct tgattcttct gacacaacag tctcgaactt     3780

aaggctagaa ttctggctac aggtaagcgc ccctaaaatc cctttgggca caatgtgtcc     3840

tgaggggaga ggcagcgacc tgtagatggg acgggggcac taaccctcag gtttggggct     3900

tctgaatgtg agtatcgcca tgtaagccca gtatttggcc aatctcagaa agctcctggt     3960

ccctggaggg atggagagag aaaaacaaac agctcctgga gcagggagag tgctggcctc     4020

ttgctctccg gctccctctg ttgccctctg gtttctcccc aggctcccgg acgtcctcta     4080

gccaccatga cttcgaaagt ttatgatcca gaacaaagga aacggatgat aactggtccg     4140

cagtggtggg ccagatgtaa acaaatgaat gttcttgatt catttattaa ttattatgat     4200

tcagaaaaac atgcagaaaa tgctgttatt tttttacatg gtaacgcggc ctcttcttat     4260

ttatggcgac atgttgtgcc acatattgag ccagtagcgc ggtgtattat accagacctt     4320

attggtatgg gcaaatcagg caaatctggt aatggttctt ataggttact tgatcattac     4380

aaatatctta ctgcatggtt tgaacttctt aatttaccaa agaagatcat ttttgtcggc     4440

catgattggg gtgcttgttt ggcatttcat tatagctatg agcatcaaga taagatcaaa     4500

gcaatagttc acgctgaaag tgtagtagat gtgattgaat catgggatga atggcctgat     4560

attgaagaag atattgcgtt gatcaaatct gaagaaggag aaaaaatggt tttggagaat     4620

aacttcttcg tggaaaccat gttgccatca aaaatcatga gaaagttaga accagaagaa     4680

tttgcagcat atcttgaacc attcaaagag aaaggtgaag ttcgtcgtcc aacattatca     4740

tggcctcgtg aaatcccgtt agtaaaaggt ggtaaacctg acgttgtaca aattgttagg     4800

aattataatg cttatctacg tgcaagtgat gatttaccaa aaatgtttat tgaatcggac     4860

ccaggattct tttccaatgc tattgttgaa ggtgccaaga agtttcctaa tactgaattt     4920

gtcaaagtaa aaggtcttca tttttcgcaa gaagatgcac ctgatgaaat gggaaaatat     4980

atcaaatcgt tcgttgagcg agttctcaaa aatgaacaat aattctagcc ctgaataagt     5040

gataataagc ggatgaatgg cagaaattcg tcgaagcgca ataaaatatc tttattttca     5100

ttacatctgt gtgttggttt tttgtgtgaa tcgatagcga taaggatcgg aagatccgcg     5160

tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc     5220

cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac     5280

aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac     5340

gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa     5400

tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt     5460

tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc     5520

ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc     5580

ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa     5640

aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg     5700

gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag     5760

ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc     5820

gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta     5880

cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg     5940

cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca     6000

acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac     6060

caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat     6120

taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg     6180

ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata     6240

aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta     6300

agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa     6360

atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag     6420

tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg     6480

tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact     6540

gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg     6600

taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc     6660

aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata     6720

ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta     6780

catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc     6840

ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg     6900

ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac     6960

agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg     7020

taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt     7080

atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct     7140

cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg     7200

ccttttgctg gccttttgct cacatggctc gacagatct                            7239


<210>  9
<211>  454
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gal4 ASK1(c) fusion protein sequence

<400>  9

Met Lys Leu Leu Ser Ser Ile Glu Gln Ala Cys Asp Ile Cys Arg Leu 
1               5                   10                  15      


Lys Lys Leu Lys Cys Ser Lys Glu Lys Pro Lys Cys Ala Lys Cys Leu 
            20                  25                  30          


Lys Asn Asn Trp Glu Cys Arg Tyr Ser Pro Lys Thr Lys Arg Ser Pro 
        35                  40                  45              


Leu Thr Arg Ala His Leu Thr Glu Val Glu Ser Arg Leu Glu Arg Leu 
    50                  55                  60                  


Glu Gln Leu Phe Leu Leu Ile Phe Pro Arg Glu Asp Leu Asp Met Ile 
65                  70                  75                  80  


Leu Lys Met Asp Ser Leu Gln Asp Ile Lys Ala Leu Leu Thr Gly Leu 
                85                  90                  95      


Phe Val Gln Asp Asn Val Asn Lys Asp Ala Val Thr Asp Arg Leu Ala 
            100                 105                 110         


Ser Val Glu Thr Asp Met Pro Leu Thr Leu Arg Gln His Arg Ile Ser 
        115                 120                 125             


Ala Thr Ser Ser Ser Glu Glu Ser Ser Asn Lys Gly Gln Arg Gln Leu 
    130                 135                 140                 


Thr Val Ser Pro Glu Phe Pro Gly Ile Arg Ser Ser Val Arg Gly Val 
145                 150                 155                 160 


Ser Ile Ser Lys Phe Glu Glu Arg Cys Cys Phe Leu Tyr Val Leu His 
                165                 170                 175     


Asn Ser Asp Asp Phe Gln Ile Tyr Phe Cys Thr Glu Leu His Cys Lys 
            180                 185                 190         


Lys Phe Phe Glu Met Val Asn Thr Ile Thr Glu Glu Lys Gly Arg Ser 
        195                 200                 205             


Thr Glu Glu Gly Asp Cys Glu Ser Asp Leu Leu Glu Tyr Asp Tyr Glu 
    210                 215                 220                 


Tyr Asp Glu Asn Gly Asp Arg Val Val Leu Gly Lys Gly Thr Tyr Gly 
225                 230                 235                 240 


Ile Val Tyr Ala Gly Arg Asp Leu Ser Asn Gln Val Arg Ile Ala Ile 
                245                 250                 255     


Lys Glu Ile Pro Glu Arg Asp Ser Arg Tyr Ser Gln Pro Leu His Glu 
            260                 265                 270         


Glu Ile Ala Leu His Lys His Leu Lys His Lys Asn Ile Val Gln Tyr 
        275                 280                 285             


Leu Gly Ser Phe Ser Glu Asn Gly Phe Ile Lys Ile Phe Met Glu Gln 
    290                 295                 300                 


Val Pro Gly Gly Ser Leu Ser Ala Leu Leu Arg Ser Lys Trp Gly Pro 
305                 310                 315                 320 


Leu Lys Asp Asn Glu Gln Thr Ile Gly Phe Tyr Thr Lys Gln Ile Leu 
                325                 330                 335     


Glu Gly Leu Lys Tyr Leu His Asp Asn Gln Ile Val His Arg Asp Ile 
            340                 345                 350         


Lys Gly Asp Asn Val Leu Ile Asn Thr Tyr Ser Gly Val Leu Lys Ile 
        355                 360                 365             


Ser Asp Phe Gly Thr Ser Lys Arg Leu Ala Gly Ile Asn Pro Cys Thr 
    370                 375                 380                 


Glu Thr Phe Thr Gly Thr Leu Gln Tyr Met Ala Pro Glu Ile Ile Asp 
385                 390                 395                 400 


Lys Gly Pro Arg Gly Tyr Gly Lys Ala Ala Asp Ile Trp Ser Leu Gly 
                405                 410                 415     


Cys Thr Ile Ile Glu Met Ala Thr Gly Lys Pro Pro Phe Tyr Glu Leu 
            420                 425                 430         


Gly Glu Pro Gln Ala Ala Met Phe Lys Val Gly Met Phe Lys Val His 
        435                 440                 445             


Pro Glu Ile Pro Ser Arg 
    450                 


<210>  10
<211>  6918
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct sequence containing ASK1(d) cloned in pBIND

<400>  10
tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta       60

ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc      120

aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg      180

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      240

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      300

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      360

ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga      420

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg      480

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac      540

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt      600

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg      660

cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata      720

agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac      780

agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt      840

gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa      900

ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact      960

cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac     1020

aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact     1080

ataggctagc cagcttgaag caagcctcct gaaagatgaa gctactgtct tctatcgaac     1140

aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg     1200

ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc     1260

tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc     1320

tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata     1380

taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag     1440

atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg     1500

cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgccgg     1560

aattcccggg gatccgtgca gcagacatct ggtctctggg ctgtacaatc attgaaatgg     1620

ccacaggaaa acccccattt tatgaactgg gagaaccaca agcagctatg ttcaaggtgg     1680

gaatgtttaa agtccaccct gagatcccag agtccatgtc tgcagaggcc aaggcattca     1740

tactgaaatg ttttgaacca gatcctgaca agagagcctg tgctaacgac ttgcttgttg     1800

atgagttttt aaaagtttca agcaaaaaga aaaagacaca acctaagctt tcagctcttt     1860

cagctggatc aaatgaatat ctcaggagta tatccttgcc ggtacctgtg ctggtggagg     1920

acaccagcag cagcagtgag tacggctcag tttcacccga cacggagttg aaagtggacc     1980

ccttctcttt caaaacaaga gccaagtcct gcggagaaag agatgtcaag ggaattcgga     2040

cactcttttt gggcattcca gatgagaatt ttgaagatca cagtgctcct ccttcccctg     2100

aagaaaaaga ttctggattc ttcatgctga ggaaggacag tgagaggcga tctagatcta     2160

gagcggccgc aggtacctga ataactaagg ccgcttccct ttagtgaggg ttaatgcttc     2220

gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa     2280

aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct     2340

gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga     2400

tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc cgataaggat     2460

cgatccgggc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg     2520

cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg     2580

gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc     2640

ttcccttcct ttcacgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc     2700

cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt     2760

gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag     2820

tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg     2880

gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag     2940

ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga     3000

tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgc ggatcttccg     3060

taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc     3120

cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag     3180

gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta     3240

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc     3300

cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc     3360

ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg     3420

caaaaagctt gattcttctg acacaacagt ctcgaactta aggctagaat tctggctaca     3480

ggtaagcgcc cctaaaatcc ctttgggcac aatgtgtcct gaggggagag gcagcgacct     3540

gtagatggga cgggggcact aaccctcagg tttggggctt ctgaatgtga gtatcgccat     3600

gtaagcccag tatttggcca atctcagaaa gctcctggtc cctggaggga tggagagaga     3660

aaaacaaaca gctcctggag cagggagagt gctggcctct tgctctccgg ctccctctgt     3720

tgccctctgg tttctcccca ggctcccgga cgtcctctag ccaccatgac ttcgaaagtt     3780

tatgatccag aacaaaggaa acggatgata actggtccgc agtggtgggc cagatgtaaa     3840

caaatgaatg ttcttgattc atttattaat tattatgatt cagaaaaaca tgcagaaaat     3900

gctgttattt ttttacatgg taacgcggcc tcttcttatt tatggcgaca tgttgtgcca     3960

catattgagc cagtagcgcg gtgtattata ccagacctta ttggtatggg caaatcaggc     4020

aaatctggta atggttctta taggttactt gatcattaca aatatcttac tgcatggttt     4080

gaacttctta atttaccaaa gaagatcatt tttgtcggcc atgattgggg tgcttgtttg     4140

gcatttcatt atagctatga gcatcaagat aagatcaaag caatagttca cgctgaaagt     4200

gtagtagatg tgattgaatc atgggatgaa tggcctgata ttgaagaaga tattgcgttg     4260

atcaaatctg aagaaggaga aaaaatggtt ttggagaata acttcttcgt ggaaaccatg     4320

ttgccatcaa aaatcatgag aaagttagaa ccagaagaat ttgcagcata tcttgaacca     4380

ttcaaagaga aaggtgaagt tcgtcgtcca acattatcat ggcctcgtga aatcccgtta     4440

gtaaaaggtg gtaaacctga cgttgtacaa attgttagga attataatgc ttatctacgt     4500

gcaagtgatg atttaccaaa aatgtttatt gaatcggacc caggattctt ttccaatgct     4560

attgttgaag gtgccaagaa gtttcctaat actgaatttg tcaaagtaaa aggtcttcat     4620

ttttcgcaag aagatgcacc tgatgaaatg ggaaaatata tcaaatcgtt cgttgagcga     4680

gttctcaaaa atgaacaata attctagccc tgaataagtg ataataagcg gatgaatggc     4740

agaaattcgt cgaagcgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt     4800

ttgtgtgaat cgatagcgat aaggatcgga agatccgcgt atggtgcact ctcagtacaa     4860

tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc     4920

cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga     4980

gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg     5040

tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg     5100

gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa     5160

atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga     5220

agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc     5280

ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg     5340

gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc     5400

gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat     5460

tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg     5520

acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag     5580

aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa     5640

cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc     5700

gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca     5760

cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc     5820

tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc     5880

tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg     5940

ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta     6000

tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag     6060

gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga     6120

ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc     6180

tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa     6240

agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa     6300

aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc     6360

cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt     6420

agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc     6480

tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac     6540

gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca     6600

gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg     6660

ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag     6720

gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt     6780

ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat     6840

ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc     6900

acatggctcg acagatct                                                   6918


<210>  11
<211>  347
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gal4 ASK1(d) fusion protein sequence

<400>  11

Met Lys Leu Leu Ser Ser Ile Glu Gln Ala Cys Asp Ile Cys Arg Leu 
1               5                   10                  15      


Lys Lys Leu Lys Cys Ser Lys Glu Lys Pro Lys Cys Ala Lys Cys Leu 
            20                  25                  30          


Lys Asn Asn Trp Glu Cys Arg Tyr Ser Pro Lys Thr Lys Arg Ser Pro 
        35                  40                  45              


Leu Thr Arg Ala His Leu Thr Glu Val Glu Ser Arg Leu Glu Arg Leu 
    50                  55                  60                  


Glu Gln Leu Phe Leu Leu Ile Phe Pro Arg Glu Asp Leu Asp Met Ile 
65                  70                  75                  80  


Leu Lys Met Asp Ser Leu Gln Asp Ile Lys Ala Leu Leu Thr Gly Leu 
                85                  90                  95      


Phe Val Gln Asp Asn Val Asn Lys Asp Ala Val Thr Asp Arg Leu Ala 
            100                 105                 110         


Ser Val Glu Thr Asp Met Pro Leu Thr Leu Arg Gln His Arg Ile Ser 
        115                 120                 125             


Ala Thr Ser Ser Ser Glu Glu Ser Ser Asn Lys Gly Gln Arg Gln Leu 
    130                 135                 140                 


Thr Val Ser Pro Glu Phe Pro Gly Ile Arg Ala Ala Asp Ile Trp Ser 
145                 150                 155                 160 


Leu Gly Cys Thr Ile Ile Glu Met Ala Thr Gly Lys Pro Pro Phe Tyr 
                165                 170                 175     


Glu Leu Gly Glu Pro Gln Ala Ala Met Phe Lys Val Gly Met Phe Lys 
            180                 185                 190         


Val His Pro Glu Ile Pro Glu Ser Met Ser Ala Glu Ala Lys Ala Phe 
        195                 200                 205             


Ile Leu Lys Cys Phe Glu Pro Asp Pro Asp Lys Arg Ala Cys Ala Asn 
    210                 215                 220                 


Asp Leu Leu Val Asp Glu Phe Leu Lys Val Ser Ser Lys Lys Lys Lys 
225                 230                 235                 240 


Thr Gln Pro Lys Leu Ser Ala Leu Ser Ala Gly Ser Asn Glu Tyr Leu 
                245                 250                 255     


Arg Ser Ile Ser Leu Pro Val Pro Val Leu Val Glu Asp Thr Ser Ser 
            260                 265                 270         


Ser Ser Glu Tyr Gly Ser Val Ser Pro Asp Thr Glu Leu Lys Val Asp 
        275                 280                 285             


Pro Phe Ser Phe Lys Thr Arg Ala Lys Ser Cys Gly Glu Arg Asp Val 
    290                 295                 300                 


Lys Gly Ile Arg Thr Leu Phe Leu Gly Ile Pro Asp Glu Asn Phe Glu 
305                 310                 315                 320 


Asp His Ser Ala Pro Pro Ser Pro Glu Glu Lys Asp Ser Gly Phe Phe 
                325                 330                 335     


Met Leu Arg Lys Asp Ser Glu Arg Arg Ser Arg 
            340                 345         


<210>  12
<211>  7171
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct sequence containing ASK1(e) cloned in pBIND

<400>  12
tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta       60

ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc      120

aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg      180

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      240

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      300

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      360

ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga      420

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg      480

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac      540

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt      600

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg      660

cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata      720

agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac      780

agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt      840

gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa      900

ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact      960

cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac     1020

aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact     1080

ataggctagc cagcttgaag caagcctcct gaaagatgaa gctactgtct tctatcgaac     1140

aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg     1200

ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc     1260

tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc     1320

tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata     1380

taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag     1440

atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg     1500

cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgccgg     1560

aattcccggg gatccatgaa gctactgtct tctatcgaac aagcatgcga tatttgccga     1620

cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg ccaagtgtct gaagaacaac     1680

tgggagtgtc gctactctcc caaaaccaaa aggtctccgc tgactagggc acatctgaca     1740

gaagtggaat caaggctaga aagactggaa cagctatttc tactgatttt tcctcgagaa     1800

gaccttgaca tgattttgaa aatggattct ttacaggata taaaagcatt gttaacagga     1860

ttatttgtac aagataatgt gaataaagat gccgtcacag atagattggc ttcagtggag     1920

actgatatgc ctctaacatt gagacagcat agaataagtg cgacatcatc atcggaagag     1980

agtagtaaca aaggtcaaag acagttgact gtatcgccgg aattcccggg gatcctgacg     2040

gaagaccaag acaaaattgt gagaaaccta atggaatctt tagctcaggg ggctgaagaa     2100

ccgaaactaa aatgggaaca catcacaacc ctcattgcaa gcctcagaga atttgtgaga     2160

tccactgacc gaaaaatcat agccaccaca ctgtcaaagc tgaaactgga gctggacttc     2220

gacagccatg gcattagcca agtccaggtg gtactctttg gttttcaaga tgctgtcaat     2280

aaagttcttc ggaatcataa catcaagccg cactggatgt ttgccttaga cagtatcatt     2340

cggaaggcgg tacagacagc cattaccatc ctggttccag aactaaggcc acatttcagc     2400

cttgcatctt ctagagcggc cgcaggtacc tgaataacta aggccgcttc cctttagtga     2460

gggttaatgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact     2520

agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta     2580

accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag     2640

gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa     2700

atccgataag gatcgatccg ggctggcgta atagcgaaga ggcccgcacc gatcgccctt     2760

cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc     2820

ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc     2880

tcctttcgct ttcttccctt cctttcacgc cacgttcgcc ggctttcccc gtcaagctct     2940

aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa     3000

acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc     3060

tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact     3120

caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg     3180

gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct     3240

tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata     3300

cgcggatctt ccgtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag     3360

ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt     3420

agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca     3480

tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa     3540

ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag     3600

aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag     3660

gcctaggctt ttgcaaaaag cttgattctt ctgacacaac agtctcgaac ttaaggctag     3720

aattctggct acaggtaagc gcccctaaaa tccctttggg cacaatgtgt cctgagggga     3780

gaggcagcga cctgtagatg ggacgggggc actaaccctc aggtttgggg cttctgaatg     3840

tgagtatcgc catgtaagcc cagtatttgg ccaatctcag aaagctcctg gtccctggag     3900

ggatggagag agaaaaacaa acagctcctg gagcagggag agtgctggcc tcttgctctc     3960

cggctccctc tgttgccctc tggtttctcc ccaggctccc ggacgtcctc tagccaccat     4020

gacttcgaaa gtttatgatc cagaacaaag gaaacggatg ataactggtc cgcagtggtg     4080

ggccagatgt aaacaaatga atgttcttga ttcatttatt aattattatg attcagaaaa     4140

acatgcagaa aatgctgtta tttttttaca tggtaacgcg gcctcttctt atttatggcg     4200

acatgttgtg ccacatattg agccagtagc gcggtgtatt ataccagacc ttattggtat     4260

gggcaaatca ggcaaatctg gtaatggttc ttataggtta cttgatcatt acaaatatct     4320

tactgcatgg tttgaacttc ttaatttacc aaagaagatc atttttgtcg gccatgattg     4380

gggtgcttgt ttggcatttc attatagcta tgagcatcaa gataagatca aagcaatagt     4440

tcacgctgaa agtgtagtag atgtgattga atcatgggat gaatggcctg atattgaaga     4500

agatattgcg ttgatcaaat ctgaagaagg agaaaaaatg gttttggaga ataacttctt     4560

cgtggaaacc atgttgccat caaaaatcat gagaaagtta gaaccagaag aatttgcagc     4620

atatcttgaa ccattcaaag agaaaggtga agttcgtcgt ccaacattat catggcctcg     4680

tgaaatcccg ttagtaaaag gtggtaaacc tgacgttgta caaattgtta ggaattataa     4740

tgcttatcta cgtgcaagtg atgatttacc aaaaatgttt attgaatcgg acccaggatt     4800

cttttccaat gctattgttg aaggtgccaa gaagtttcct aatactgaat ttgtcaaagt     4860

aaaaggtctt catttttcgc aagaagatgc acctgatgaa atgggaaaat atatcaaatc     4920

gttcgttgag cgagttctca aaaatgaaca ataattctag ccctgaataa gtgataataa     4980

gcggatgaat ggcagaaatt cgtcgaagcg caataaaata tctttatttt cattacatct     5040

gtgtgttggt tttttgtgtg aatcgatagc gataaggatc ggaagatccg cgtatggtgc     5100

actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca     5160

cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg     5220

accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga     5280

cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct     5340

tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc     5400

taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa     5460

tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt     5520

gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct     5580

gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc     5640

cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta     5700

tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac     5760

tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc     5820

atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac     5880

ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg     5940

gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac     6000

gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc     6060

gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt     6120

gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga     6180

gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc     6240

cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag     6300

atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca     6360

tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc     6420

ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca     6480

gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc     6540

tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta     6600

ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt     6660

ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc     6720

gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg     6780

ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg     6840

tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag     6900

ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc     6960

agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat     7020

agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg     7080

gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc     7140

tggccttttg ctcacatggc tcgacagatc t                                    7171


<210>  13
<211>  278
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gal4 ASK1(e) fusion protein sequence

<400>  13

Met Lys Leu Leu Ser Ser Ile Glu Gln Ala Cys Asp Ile Cys Arg Leu 
1               5                   10                  15      


Lys Lys Leu Lys Cys Ser Lys Glu Lys Pro Lys Cys Ala Lys Cys Leu 
            20                  25                  30          


Lys Asn Asn Trp Glu Cys Arg Tyr Ser Pro Lys Thr Lys Arg Ser Pro 
        35                  40                  45              


Leu Thr Arg Ala His Leu Thr Glu Val Glu Ser Arg Leu Glu Arg Leu 
    50                  55                  60                  


Glu Gln Leu Phe Leu Leu Ile Phe Pro Arg Glu Asp Leu Asp Met Ile 
65                  70                  75                  80  


Leu Lys Met Asp Ser Leu Gln Asp Ile Lys Ala Leu Leu Thr Gly Leu 
                85                  90                  95      


Phe Val Gln Asp Asn Val Asn Lys Asp Ala Val Thr Asp Arg Leu Ala 
            100                 105                 110         


Ser Val Glu Thr Asp Met Pro Leu Thr Leu Arg Gln His Arg Ile Ser 
        115                 120                 125             


Ala Thr Ser Ser Ser Glu Glu Ser Ser Asn Lys Gly Gln Arg Gln Leu 
    130                 135                 140                 


Thr Val Ser Pro Glu Phe Pro Gly Ile Arg Thr Glu Asp Gln Asp Lys 
145                 150                 155                 160 


Ile Val Arg Asn Leu Met Glu Ser Leu Ala Gln Gly Ala Glu Glu Pro 
                165                 170                 175     


Lys Leu Lys Trp Glu His Ile Thr Thr Leu Ile Ala Ser Leu Arg Glu 
            180                 185                 190         


Phe Val Arg Ser Thr Asp Arg Lys Ile Ile Ala Thr Thr Leu Ser Lys 
        195                 200                 205             


Leu Lys Leu Glu Leu Asp Phe Asp Ser His Gly Ile Ser Gln Val Gln 
    210                 215                 220                 


Val Val Leu Phe Gly Phe Gln Asp Ala Val Asn Lys Val Leu Arg Asn 
225                 230                 235                 240 


His Asn Ile Lys Pro His Trp Met Phe Ala Leu Asp Ser Ile Ile Arg 
                245                 250                 255     


Lys Ala Val Gln Thr Ala Ile Thr Ile Leu Val Pro Glu Leu Arg Pro 
            260                 265                 270         


His Phe Ser Leu Ala Ser 
        275             


<210>  14
<211>  6981
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct sequence containing ASK1(f) cloned in pBIND

<400>  14
tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta       60

ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc      120

aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg      180

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      240

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      300

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      360

ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga      420

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg      480

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac      540

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt      600

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg      660

cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata      720

agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac      780

agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt      840

gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa      900

ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact      960

cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac     1020

aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact     1080

ataggctagc cagcttgaag caagcctcct gaaagatgaa gctactgtct tctatcgaac     1140

aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg     1200

ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc     1260

tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc     1320

tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata     1380

taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag     1440

atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg     1500

cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgccgg     1560

aattcccggg gatccgtgac agtatcattc ggaaggcggt acagacagcc attaccatcc     1620

tggttccaga actaaggcca catttcagcc ttgcatctga gagtgatact gctgatcaag     1680

aagacttgga tgtagaagat gaccatgagg aacagccttc aaatcaaact gtccgaagac     1740

ctcaggctgt cattgaagat gctgtggcta cctcaggcgt gagcacgctc agttctactg     1800

tgtctcatga ttcccagagt gctcaccggt cactgaatgt acagcttgga aggatgaaaa     1860

tagaaaccaa tagattactg gaagaattgg ttcggaaaga gaaagaatta caagcactcc     1920

ttcatcgagc tattgaagaa aaagaccaag aaattaaaca cctgaagctt aagtcccaac     1980

ccatagaaat tcctgaattg cctgtatttc atctaaattc ttctggcaca aatactgaag     2040

attctgaact taccgactgg ctgagagtga atggagctga tgaagacact ataagccggt     2100

ttttggctga agattataca ctattggatg ttctctacta tgttacacgt gatgacttaa     2160

aatgcttgag actaagggga gggatgctgt gcacactgtg gaaggctatt gactctagat     2220

ctagagcggc cgcaggtacc tgaataacta aggccgcttc cctttagtga gggttaatgc     2280

ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt     2340

gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa     2400

gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg     2460

agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atccgataag     2520

gatcgatccg ggctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt     2580

gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg     2640

gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct     2700

ttcttccctt cctttcacgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg     2760

ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag     2820

ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg     2880

gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc     2940

tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat     3000

gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc     3060

tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgcggatctt     3120

ccgtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa     3180

gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac     3240

caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa     3300

ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag     3360

ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc     3420

cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt     3480

ttgcaaaaag cttgattctt ctgacacaac agtctcgaac ttaaggctag aattctggct     3540

acaggtaagc gcccctaaaa tccctttggg cacaatgtgt cctgagggga gaggcagcga     3600

cctgtagatg ggacgggggc actaaccctc aggtttgggg cttctgaatg tgagtatcgc     3660

catgtaagcc cagtatttgg ccaatctcag aaagctcctg gtccctggag ggatggagag     3720

agaaaaacaa acagctcctg gagcagggag agtgctggcc tcttgctctc cggctccctc     3780

tgttgccctc tggtttctcc ccaggctccc ggacgtcctc tagccaccat gacttcgaaa     3840

gtttatgatc cagaacaaag gaaacggatg ataactggtc cgcagtggtg ggccagatgt     3900

aaacaaatga atgttcttga ttcatttatt aattattatg attcagaaaa acatgcagaa     3960

aatgctgtta tttttttaca tggtaacgcg gcctcttctt atttatggcg acatgttgtg     4020

ccacatattg agccagtagc gcggtgtatt ataccagacc ttattggtat gggcaaatca     4080

ggcaaatctg gtaatggttc ttataggtta cttgatcatt acaaatatct tactgcatgg     4140

tttgaacttc ttaatttacc aaagaagatc atttttgtcg gccatgattg gggtgcttgt     4200

ttggcatttc attatagcta tgagcatcaa gataagatca aagcaatagt tcacgctgaa     4260

agtgtagtag atgtgattga atcatgggat gaatggcctg atattgaaga agatattgcg     4320

ttgatcaaat ctgaagaagg agaaaaaatg gttttggaga ataacttctt cgtggaaacc     4380

atgttgccat caaaaatcat gagaaagtta gaaccagaag aatttgcagc atatcttgaa     4440

ccattcaaag agaaaggtga agttcgtcgt ccaacattat catggcctcg tgaaatcccg     4500

ttagtaaaag gtggtaaacc tgacgttgta caaattgtta ggaattataa tgcttatcta     4560

cgtgcaagtg atgatttacc aaaaatgttt attgaatcgg acccaggatt cttttccaat     4620

gctattgttg aaggtgccaa gaagtttcct aatactgaat ttgtcaaagt aaaaggtctt     4680

catttttcgc aagaagatgc acctgatgaa atgggaaaat atatcaaatc gttcgttgag     4740

cgagttctca aaaatgaaca ataattctag ccctgaataa gtgataataa gcggatgaat     4800

ggcagaaatt cgtcgaagcg caataaaata tctttatttt cattacatct gtgtgttggt     4860

tttttgtgtg aatcgatagc gataaggatc ggaagatccg cgtatggtgc actctcagta     4920

caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg     4980

cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg     5040

ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc     5100

tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag     5160

gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt     5220

caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa     5280

ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt     5340

gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt     5400

tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt     5460

ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg     5520

tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga     5580

atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa     5640

gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga     5700

caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa     5760

ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca     5820

ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta     5880

ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac     5940

ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc     6000

gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag     6060

ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga     6120

taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt     6180

agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata     6240

atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag     6300

aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa     6360

caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt     6420

ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc     6480

cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa     6540

tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa     6600

gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc     6660

ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa     6720

gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa     6780

caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg     6840

ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc     6900

tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg     6960

ctcacatggc tcgacagatc t                                               6981


<210>  15
<211>  368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gal4 ASK1(f) fusion protein sequence

<400>  15

Met Lys Leu Leu Ser Ser Ile Glu Gln Ala Cys Asp Ile Cys Arg Leu 
1               5                   10                  15      


Lys Lys Leu Lys Cys Ser Lys Glu Lys Pro Lys Cys Ala Lys Cys Leu 
            20                  25                  30          


Lys Asn Asn Trp Glu Cys Arg Tyr Ser Pro Lys Thr Lys Arg Ser Pro 
        35                  40                  45              


Leu Thr Arg Ala His Leu Thr Glu Val Glu Ser Arg Leu Glu Arg Leu 
    50                  55                  60                  


Glu Gln Leu Phe Leu Leu Ile Phe Pro Arg Glu Asp Leu Asp Met Ile 
65                  70                  75                  80  


Leu Lys Met Asp Ser Leu Gln Asp Ile Lys Ala Leu Leu Thr Gly Leu 
                85                  90                  95      


Phe Val Gln Asp Asn Val Asn Lys Asp Ala Val Thr Asp Arg Leu Ala 
            100                 105                 110         


Ser Val Glu Thr Asp Met Pro Leu Thr Leu Arg Gln His Arg Ile Ser 
        115                 120                 125             


Ala Thr Ser Ser Ser Glu Glu Ser Ser Asn Lys Gly Gln Arg Gln Leu 
    130                 135                 140                 


Thr Val Ser Pro Glu Phe Pro Gly Ile Arg Asp Ser Ile Ile Arg Lys 
145                 150                 155                 160 


Ala Val Gln Thr Ala Ile Thr Ile Leu Val Pro Glu Leu Arg Pro His 
                165                 170                 175     


Phe Ser Leu Ala Ser Glu Ser Asp Thr Ala Asp Gln Glu Asp Leu Asp 
            180                 185                 190         


Val Glu Asp Asp His Glu Glu Gln Pro Ser Asn Gln Thr Val Arg Arg 
        195                 200                 205             


Pro Gln Ala Val Ile Glu Asp Ala Val Ala Thr Ser Gly Val Ser Thr 
    210                 215                 220                 


Leu Ser Ser Thr Val Ser His Asp Ser Gln Ser Ala His Arg Ser Leu 
225                 230                 235                 240 


Asn Val Gln Leu Gly Arg Met Lys Ile Glu Thr Asn Arg Leu Leu Glu 
                245                 250                 255     


Glu Leu Val Arg Lys Glu Lys Glu Leu Gln Ala Leu Leu His Arg Ala 
            260                 265                 270         


Ile Glu Glu Lys Asp Gln Glu Ile Lys His Leu Lys Leu Lys Ser Gln 
        275                 280                 285             


Pro Ile Glu Ile Pro Glu Leu Pro Val Phe His Leu Asn Ser Ser Gly 
    290                 295                 300                 


Thr Asn Thr Glu Asp Ser Glu Leu Thr Asp Trp Leu Arg Val Asn Gly 
305                 310                 315                 320 


Ala Asp Glu Asp Thr Ile Ser Arg Phe Leu Ala Glu Asp Tyr Thr Leu 
                325                 330                 335     


Leu Asp Val Leu Tyr Tyr Val Thr Arg Asp Asp Leu Lys Cys Leu Arg 
            340                 345                 350         


Leu Arg Gly Gly Met Leu Cys Thr Leu Trp Lys Ala Ile Asp Ser Arg 
        355                 360                 365             


<210>  16
<211>  9485
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct sequence containing ASK1(g ) cloned in pBIND

<400>  16
tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta       60

ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc      120

aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg      180

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      240

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      300

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      360

ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga      420

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg      480

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac      540

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt      600

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg      660

cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata      720

agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac      780

agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt      840

gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa      900

ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact      960

cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac     1020

aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact     1080

ataggctagc cagcttgaag caagcctcct gaaagatgaa gctactgtct tctatcgaac     1140

aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg     1200

ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc     1260

tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc     1320

tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata     1380

taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag     1440

atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg     1500

cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgccgg     1560

aattcccggg gatccgtacg gaggcggacg agggcatcac tttctctgtg ccacccttcg     1620

ccccctcggg cttctgcacc atccccgagg gcggcatctg caggagggga ggagcggcgg     1680

cggtgggcga gggcgaggag caccagctgc caccgccgcc gccgggcagt ttctggaacg     1740

tggagagcgc cgctgcccct ggcatcggtt gtccggcggc cacctcctcg agcagtgcca     1800

cccgaggccg gggcagctct gttggcgggg gcagccgacg gaccacggtg gcatatgtga     1860

tcaacgaagc gagccaaggg caactggtgg tggccgagag cgaggccctg cagagcttgc     1920

gggaggcgtg cgagacagtg ggcgccaccc tggaaaccct gcattttggg aaactcgact     1980

ttggagaaac caccgtgctg gaccgctttt acaatgcaga tattgcggtg gtggagatga     2040

gcgatgcctt ccggcagccg tccttgtttt accaccttgg ggtgagagaa agtttcagca     2100

tggccaacaa catcatcctc tactgtgata ctaactcgga ctctctgcag tcactgaagg     2160

aaatcatttg ccagaagaat actatgtgca ctgggaacta cacctttgtt ccttacatga     2220

taactccaca taacaaagtc tactgctgtg acagcagctt catgaagggg ttgacagagc     2280

tcatgcaacc gaacttcgag ctgcttcttg gacccatctg cttacctctt gtggatcgtt     2340

ttattcaact tttgaaggtg gcacaagcaa gttctagcca gtacttccgg gaatctatac     2400

tcaatgacat caggaaagct cgtaatttat acactggtaa agaattggca gctgagttgg     2460

caagaattcg gcagcgagta gataatatcg aagtcttgac agcagatatt gtcataaatc     2520

tgttactttc ctacagagat atccaggact atgattctat tgtgaagctg gtagagactt     2580

tagaaaaact gccaaccttt gatttggcct cccatcacca tgtgaagttt cattatgcat     2640

ttgcactgaa taggagaaat ctccctggtg acagagcaaa agctcttgat attatgattc     2700

ccatggtgca aagcgaagga caagttgctt cagatatgta ttgcctagtt ggtcgaatct     2760

acaaagatat gtttttggac tctaatttca cggacactga aagcagagac catggagctt     2820

cttggttcaa aaaggcattt gaatctgagc caacactaca gtcaggaatt aattatgcgg     2880

tcctcctcct ggcagctgga caccagtttg aatcttcctt tgagctccgg aaagttgggg     2940

tgaagctaag tagtcttctt ggtaaaaagg gaaacttgga aaaactccag agctactggg     3000

aagttggatt ttttctgggg gccagcgtcc tagccaatga ccacatgaga gtcattcaag     3060

catctgaaaa gctttttaaa ctgaagacac cagcatggta cctcaagtct attgtagaga     3120

caattttaat atataagcat tttgtgaaac tgaccacaga acagcctgtg gccaagcaag     3180

aacttgtgga cttttggatg gatttcctgg tcgaggccac aaagacagat gttactgtgg     3240

ttaggtttcc agtattaata ttagaaccaa ccaaaatcta tcaaccttct tatttgtcta     3300

tcaacaatga agttgaggaa aagacaatct ctatttggca cgtgcttcct gatgacaaga     3360

aaggtataca tgagtggaat tttagtgcct cttctgtcag gggagtgagt atttctaaat     3420

ttgaagaaag atgctgcttt ctttatgtgc ttcacaattc tgatgatttc caaatctatt     3480

tctgtacaga acttcattgt aaaaagtttt ttgagatggt gaacaccatt accgaagaga     3540

aggggagaag cacagaggaa ggagactgtg aaagtgactt gctggagtat gactatgaat     3600

atgatgaaaa tggtgacaga gtcgttttag gaaaaggcac ttatgggata gtctacgcag     3660

gtcgggactt gagcaaccaa gtcagaattg ctattaagga aatcccagag agagacagca     3720

gatactctca gcccctgcat gaagaaatag cattgcataa acacctgaag cacaaaaata     3780

ttgtccagta tctgggctct ttcagtgaga atggtttcat taaaatcttc atggagcagg     3840

tccctggagg aagtctttct gctctccttc gttccaaatg gggtccatta aaagacaatg     3900

agcaaacaat tggcttttat acaaagcaaa tactggaagg attaaaatat ctccatgaca     3960

atcagatagt tcaccgggac ataaagggtg acaatgtgtt gattaatacc tacagtggtg     4020

ttctcaagat ctctgacttc ggaacatcaa agaggcttgc tggcataaac ccctgtactg     4080

aaacttttac tggtaccctc cagtatatgg caccagaaat aatagataaa ggaccaagag     4140

gctacggaaa agcagcagac atctggtctc tgggctgtac aatcattgaa atggccacag     4200

gaaaaccccc attttatgaa ctgggagaac cacaagcagc tatgttcaag gtgggaatgt     4260

ttaaagtcca ccctgagatc ccagagtcca tgtctgcaga ggccaaggca ttcatactga     4320

aatgttttga accagatcct gacaagagag cctgtgctaa cgacttgctt gttgatgagt     4380

ttttaaaagt ttcaagcaaa aagaaaaaga cacaacctaa gctttcagct ctttcagctg     4440

gatcaaatga atatctcagg agtatatcct tgccggtacc tgtgctggtg gaggacacca     4500

gcagcagcag tgagtacggc tcagtttcac ccgacacgga gttgaaagtg gaccccttct     4560

ctttcaaaac aagagccaag tcctgcggag aaagagatgt caagggaatt cggacactct     4620

ttttgggcat tccagatgag aattttgaag atcacagtgc tcctccttcc cctgaagaaa     4680

aagattctgg attcttcatg ctgaggaagg acagtgagag gcgtctagag cggccgcagg     4740

tacctgaata actaaggccg cttcccttta gtgagggtta atgcttcgag cagacatgat     4800

aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat     4860

ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt     4920

taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt     4980

ttaaagcaag taaaacctct acaaatgtgg taaaatccga taaggatcga tccgggctgg     5040

cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc     5100

gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg     5160

tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc     5220

acgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc     5280

gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta     5340

gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta     5400

atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg     5460

atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa     5520

aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct     5580

ccttacgcat ctgtgcggta tttcacaccg catacgcgga tcttccgtac cttctgaggc     5640

ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca     5700

gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc     5760

ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata     5820

gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg     5880

ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag     5940

ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgat     6000

tcttctgaca caacagtctc gaacttaagg ctagaattct ggctacaggt aagcgcccct     6060

aaaatccctt tgggcacaat gtgtcctgag gggagaggca gcgacctgta gatgggacgg     6120

gggcactaac cctcaggttt ggggcttctg aatgtgagta tcgccatgta agcccagtat     6180

ttggccaatc tcagaaagct cctggtccct ggagggatgg agagagaaaa acaaacagct     6240

cctggagcag ggagagtgct ggcctcttgc tctccggctc cctctgttgc cctctggttt     6300

ctccccaggc tcccggacgt cctctagcca ccatgacttc gaaagtttat gatccagaac     6360

aaaggaaacg gatgataact ggtccgcagt ggtgggccag atgtaaacaa atgaatgttc     6420

ttgattcatt tattaattat tatgattcag aaaaacatgc agaaaatgct gttatttttt     6480

tacatggtaa cgcggcctct tcttatttat ggcgacatgt tgtgccacat attgagccag     6540

tagcgcggtg tattatacca gaccttattg gtatgggcaa atcaggcaaa tctggtaatg     6600

gttcttatag gttacttgat cattacaaat atcttactgc atggtttgaa cttcttaatt     6660

taccaaagaa gatcattttt gtcggccatg attggggtgc ttgtttggca tttcattata     6720

gctatgagca tcaagataag atcaaagcaa tagttcacgc tgaaagtgta gtagatgtga     6780

ttgaatcatg ggatgaatgg cctgatattg aagaagatat tgcgttgatc aaatctgaag     6840

aaggagaaaa aatggttttg gagaataact tcttcgtgga aaccatgttg ccatcaaaaa     6900

tcatgagaaa gttagaacca gaagaatttg cagcatatct tgaaccattc aaagagaaag     6960

gtgaagttcg tcgtccaaca ttatcatggc ctcgtgaaat cccgttagta aaaggtggta     7020

aacctgacgt tgtacaaatt gttaggaatt ataatgctta tctacgtgca agtgatgatt     7080

taccaaaaat gtttattgaa tcggacccag gattcttttc caatgctatt gttgaaggtg     7140

ccaagaagtt tcctaatact gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag     7200

atgcacctga tgaaatggga aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg     7260

aacaataatt ctagccctga ataagtgata ataagcggat gaatggcaga aattcgtcga     7320

agcgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga     7380

tagcgataag gatcggaaga tccgcgtatg gtgcactctc agtacaatct gctctgatgc     7440

cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg     7500

tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca     7560

gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt     7620

tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg     7680

aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct     7740

catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat     7800

tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc     7860

tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg     7920

ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg     7980

ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga     8040

cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta     8100

ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc     8160

tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc     8220

gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg     8280

ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc     8340

aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca     8400

acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct     8460

tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat     8520

cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg     8580

gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat     8640

taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact     8700

tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat     8760

cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc     8820

ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct     8880

accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg     8940

cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca     9000

cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc     9060

tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga     9120

taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac     9180

gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga     9240

agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag     9300

ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg     9360

acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag     9420

caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tggctcgaca     9480

gatct                                                                 9485


<210>  17
<211>  1202
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gal4 ASK1(g) fusion protein sequence

<400>  17

Met Lys Leu Leu Ser Ser Ile Glu Gln Ala Cys Asp Ile Cys Arg Leu 
1               5                   10                  15      


Lys Lys Leu Lys Cys Ser Lys Glu Lys Pro Lys Cys Ala Lys Cys Leu 
            20                  25                  30          


Lys Asn Asn Trp Glu Cys Arg Tyr Ser Pro Lys Thr Lys Arg Ser Pro 
        35                  40                  45              


Leu Thr Arg Ala His Leu Thr Glu Val Glu Ser Arg Leu Glu Arg Leu 
    50                  55                  60                  


Glu Gln Leu Phe Leu Leu Ile Phe Pro Arg Glu Asp Leu Asp Met Ile 
65                  70                  75                  80  


Leu Lys Met Asp Ser Leu Gln Asp Ile Lys Ala Leu Leu Thr Gly Leu 
                85                  90                  95      


Phe Val Gln Asp Asn Val Asn Lys Asp Ala Val Thr Asp Arg Leu Ala 
            100                 105                 110         


Ser Val Glu Thr Asp Met Pro Leu Thr Leu Arg Gln His Arg Ile Ser 
        115                 120                 125             


Ala Thr Ser Ser Ser Glu Glu Ser Ser Asn Lys Gly Gln Arg Gln Leu 
    130                 135                 140                 


Thr Val Ser Pro Glu Phe Pro Gly Ile Arg Thr Glu Ala Asp Glu Gly 
145                 150                 155                 160 


Ile Thr Phe Ser Val Pro Pro Phe Ala Pro Ser Gly Phe Cys Thr Ile 
                165                 170                 175     


Pro Glu Gly Gly Ile Cys Arg Arg Gly Gly Ala Ala Ala Val Gly Glu 
            180                 185                 190         


Gly Glu Glu His Gln Leu Pro Pro Pro Pro Pro Gly Ser Phe Trp Asn 
        195                 200                 205             


Val Glu Ser Ala Ala Ala Pro Gly Ile Gly Cys Pro Ala Ala Thr Ser 
    210                 215                 220                 


Ser Ser Ser Ala Thr Arg Gly Arg Gly Ser Ser Val Gly Gly Gly Ser 
225                 230                 235                 240 


Arg Arg Thr Thr Val Ala Tyr Val Ile Asn Glu Ala Ser Gln Gly Gln 
                245                 250                 255     


Leu Val Val Ala Glu Ser Glu Ala Leu Gln Ser Leu Arg Glu Ala Cys 
            260                 265                 270         


Glu Thr Val Gly Ala Thr Leu Glu Thr Leu His Phe Gly Lys Leu Asp 
        275                 280                 285             


Phe Gly Glu Thr Thr Val Leu Asp Arg Phe Tyr Asn Ala Asp Ile Ala 
    290                 295                 300                 


Val Val Glu Met Ser Asp Ala Phe Arg Gln Pro Ser Leu Phe Tyr His 
305                 310                 315                 320 


Leu Gly Val Arg Glu Ser Phe Ser Met Ala Asn Asn Ile Ile Leu Tyr 
                325                 330                 335     


Cys Asp Thr Asn Ser Asp Ser Leu Gln Ser Leu Lys Glu Ile Ile Cys 
            340                 345                 350         


Gln Lys Asn Thr Met Cys Thr Gly Asn Tyr Thr Phe Val Pro Tyr Met 
        355                 360                 365             


Ile Thr Pro His Asn Lys Val Tyr Cys Cys Asp Ser Ser Phe Met Lys 
    370                 375                 380                 


Gly Leu Thr Glu Leu Met Gln Pro Asn Phe Glu Leu Leu Leu Gly Pro 
385                 390                 395                 400 


Ile Cys Leu Pro Leu Val Asp Arg Phe Ile Gln Leu Leu Lys Val Ala 
                405                 410                 415     


Gln Ala Ser Ser Ser Gln Tyr Phe Arg Glu Ser Ile Leu Asn Asp Ile 
            420                 425                 430         


Arg Lys Ala Arg Asn Leu Tyr Thr Gly Lys Glu Leu Ala Ala Glu Leu 
        435                 440                 445             


Ala Arg Ile Arg Gln Arg Val Asp Asn Ile Glu Val Leu Thr Ala Asp 
    450                 455                 460                 


Ile Val Ile Asn Leu Leu Leu Ser Tyr Arg Asp Ile Gln Asp Tyr Asp 
465                 470                 475                 480 


Ser Ile Val Lys Leu Val Glu Thr Leu Glu Lys Leu Pro Thr Phe Asp 
                485                 490                 495     


Leu Ala Ser His His His Val Lys Phe His Tyr Ala Phe Ala Leu Asn 
            500                 505                 510         


Arg Arg Asn Leu Pro Gly Asp Arg Ala Lys Ala Leu Asp Ile Met Ile 
        515                 520                 525             


Pro Met Val Gln Ser Glu Gly Gln Val Ala Ser Asp Met Tyr Cys Leu 
    530                 535                 540                 


Val Gly Arg Ile Tyr Lys Asp Met Phe Leu Asp Ser Asn Phe Thr Asp 
545                 550                 555                 560 


Thr Glu Ser Arg Asp His Gly Ala Ser Trp Phe Lys Lys Ala Phe Glu 
                565                 570                 575     


Ser Glu Pro Thr Leu Gln Ser Gly Ile Asn Tyr Ala Val Leu Leu Leu 
            580                 585                 590         


Ala Ala Gly His Gln Phe Glu Ser Ser Phe Glu Leu Arg Lys Val Gly 
        595                 600                 605             


Val Lys Leu Ser Ser Leu Leu Gly Lys Lys Gly Asn Leu Glu Lys Leu 
    610                 615                 620                 


Gln Ser Tyr Trp Glu Val Gly Phe Phe Leu Gly Ala Ser Val Leu Ala 
625                 630                 635                 640 


Asn Asp His Met Arg Val Ile Gln Ala Ser Glu Lys Leu Phe Lys Leu 
                645                 650                 655     


Lys Thr Pro Ala Trp Tyr Leu Lys Ser Ile Val Glu Thr Ile Leu Ile 
            660                 665                 670         


Tyr Lys His Phe Val Lys Leu Thr Thr Glu Gln Pro Val Ala Lys Gln 
        675                 680                 685             


Glu Leu Val Asp Phe Trp Met Asp Phe Leu Val Glu Ala Thr Lys Thr 
    690                 695                 700                 


Asp Val Thr Val Val Arg Phe Pro Val Leu Ile Leu Glu Pro Thr Lys 
705                 710                 715                 720 


Ile Tyr Gln Pro Ser Tyr Leu Ser Ile Asn Asn Glu Val Glu Glu Lys 
                725                 730                 735     


Thr Ile Ser Ile Trp His Val Leu Pro Asp Asp Lys Lys Gly Ile His 
            740                 745                 750         


Glu Trp Asn Phe Ser Ala Ser Ser Val Arg Gly Val Ser Ile Ser Lys 
        755                 760                 765             


Phe Glu Glu Arg Cys Cys Phe Leu Tyr Val Leu His Asn Ser Asp Asp 
    770                 775                 780                 


Phe Gln Ile Tyr Phe Cys Thr Glu Leu His Cys Lys Lys Phe Phe Glu 
785                 790                 795                 800 


Met Val Asn Thr Ile Thr Glu Glu Lys Gly Arg Ser Thr Glu Glu Gly 
                805                 810                 815     


Asp Cys Glu Ser Asp Leu Leu Glu Tyr Asp Tyr Glu Tyr Asp Glu Asn 
            820                 825                 830         


Gly Asp Arg Val Val Leu Gly Lys Gly Thr Tyr Gly Ile Val Tyr Ala 
        835                 840                 845             


Gly Arg Asp Leu Ser Asn Gln Val Arg Ile Ala Ile Lys Glu Ile Pro 
    850                 855                 860                 


Glu Arg Asp Ser Arg Tyr Ser Gln Pro Leu His Glu Glu Ile Ala Leu 
865                 870                 875                 880 


His Lys His Leu Lys His Lys Asn Ile Val Gln Tyr Leu Gly Ser Phe 
                885                 890                 895     


Ser Glu Asn Gly Phe Ile Lys Ile Phe Met Glu Gln Val Pro Gly Gly 
            900                 905                 910         


Ser Leu Ser Ala Leu Leu Arg Ser Lys Trp Gly Pro Leu Lys Asp Asn 
        915                 920                 925             


Glu Gln Thr Ile Gly Phe Tyr Thr Lys Gln Ile Leu Glu Gly Leu Lys 
    930                 935                 940                 


Tyr Leu His Asp Asn Gln Ile Val His Arg Asp Ile Lys Gly Asp Asn 
945                 950                 955                 960 


Val Leu Ile Asn Thr Tyr Ser Gly Val Leu Lys Ile Ser Asp Phe Gly 
                965                 970                 975     


Thr Ser Lys Arg Leu Ala Gly Ile Asn Pro Cys Thr Glu Thr Phe Thr 
            980                 985                 990         


Gly Thr Leu Gln Tyr Met Ala Pro  Glu Ile Ile Asp Lys  Gly Pro Arg 
        995                 1000                 1005             


Gly Tyr  Gly Lys Ala Ala Asp  Ile Trp Ser Leu Gly  Cys Thr Ile 
    1010                 1015                 1020             


Ile Glu  Met Ala Thr Gly Lys  Pro Pro Phe Tyr Glu  Leu Gly Glu 
    1025                 1030                 1035             


Pro Gln  Ala Ala Met Phe Lys  Val Gly Met Phe Lys  Val His Pro 
    1040                 1045                 1050             


Glu Ile  Pro Glu Ser Met Ser  Ala Glu Ala Lys Ala  Phe Ile Leu 
    1055                 1060                 1065             


Lys Cys  Phe Glu Pro Asp Pro  Asp Lys Arg Ala Cys  Ala Asn Asp 
    1070                 1075                 1080             


Leu Leu  Val Asp Glu Phe Leu  Lys Val Ser Ser Lys  Lys Lys Lys 
    1085                 1090                 1095             


Thr Gln  Pro Lys Leu Ser Ala  Leu Ser Ala Gly Ser  Asn Glu Tyr 
    1100                 1105                 1110             


Leu Arg  Ser Ile Ser Leu Pro  Val Pro Val Leu Val  Glu Asp Thr 
    1115                 1120                 1125             


Ser Ser  Ser Ser Glu Tyr Gly  Ser Val Ser Pro Asp  Thr Glu Leu 
    1130                 1135                 1140             


Lys Val  Asp Pro Phe Ser Phe  Lys Thr Arg Ala Lys  Ser Cys Gly 
    1145                 1150                 1155             


Glu Arg  Asp Val Lys Gly Ile  Arg Thr Leu Phe Leu  Gly Ile Pro 
    1160                 1165                 1170             


Asp Glu  Asn Phe Glu Asp His  Ser Ala Pro Pro Ser  Pro Glu Glu 
    1175                 1180                 1185             


Lys Asp  Ser Gly Phe Phe Met  Leu Arg Lys Asp Ser  Glu Arg 
    1190                 1195                 1200         


<210>  18
<211>  8541
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct sequence containing ASK1(h) cloned in pBIND

<400>  18
tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta       60

ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc      120

aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg      180

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      240

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      300

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      360

ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga      420

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg      480

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac      540

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt      600

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg      660

cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata      720

agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac      780

agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt      840

gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa      900

ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact      960

cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac     1020

aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact     1080

ataggctagc cagcttgaag caagcctcct gaaagatgaa gctactgtct tctatcgaac     1140

aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg     1200

ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc     1260

tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc     1320

tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata     1380

taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag     1440

atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg     1500

cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgccgg     1560

aattcccggg gatccgttcc tacagagata tccaggacta tgattctatt gtgaagctgg     1620

tagagacttt agaaaaactg ccaacctttg atttggcctc ccatcaccat gtgaagtttc     1680

attatgcatt tgcactgaat aggagaaatc tccctggtga cagagcaaaa gctcttgata     1740

ttatgattcc catggtgcaa agcgaaggac aagttgcttc agatatgtat tgcctagttg     1800

gtcgaatcta caaagatatg tttttggact ctaatttcac ggacactgaa agcagagacc     1860

atggagcttc ttggttcaaa aaggcatttg aatctgagcc aacactacag tcaggaatta     1920

attatgcggt cctcctcctg gcagctggac accagtttga atcttccttt gagctccgga     1980

aagttggggt gaagctaagt agtcttcttg gtaaaaaggg aaacttggaa aaactccaga     2040

gctactggga agttggattt tttctggggg ccagcgtcct agccaatgac cacatgagag     2100

tcattcaagc atctgaaaag ctttttaaac tgaagacacc agcatggtac ctcaagtcta     2160

ttgtagagac aattttaata tataagcatt ttgtgaaact gaccacagaa cagcctgtgg     2220

ccaagcaaga acttgtggac ttttggatgg atttcctggt cgaggccaca aagacagatg     2280

ttactgtggt taggtttcca gtattaatat tagaaccaac caaaatctat caaccttctt     2340

atttgtctat caacaatgaa gttgaggaaa agacaatctc tatttggcac gtgcttcctg     2400

atgacaagaa aggtatacat gagtggaatt ttagtgcctc ttctgtcagg ggagtgagta     2460

tttctaaatt tgaagaaaga tgctgctttc tttatgtgct tcacaattct gatgatttcc     2520

aaatctattt ctgtacagaa cttcattgta aaaagttttt tgagatggtg aacaccatta     2580

ccgaagagaa ggggagaagc acagaggaag gagactgtga aagtgacttg ctggagtatg     2640

actatgaata tgatgaaaat ggtgacagag tcgttttagg aaaaggcact tatgggatag     2700

tctacgcagg tcgggacttg agcaaccaag tcagaattgc tattaaggaa atcccagaga     2760

gagacagcag atactctcag cccctgcatg aagaaatagc attgcataaa cacctgaagc     2820

acaaaaatat tgtccagtat ctgggctctt tcagtgagaa tggtttcatt aaaatcttca     2880

tggagcaggt ccctggagga agtctttctg ctctccttcg ttccaaatgg ggtccattaa     2940

aagacaatga gcaaacaatt ggcttttata caaagcaaat actggaagga ttaaaatatc     3000

tccatgacaa tcagatagtt caccgggaca taaagggtga caatgtgttg attaatacct     3060

acagtggtgt tctcaagatc tctgacttcg gaacatcaaa gaggcttgct ggcataaacc     3120

cctgtactga aacttttact ggtaccctcc agtatatggc accagaaata atagataaag     3180

gaccaagagg ctacggaaaa gcagcagaca tctggtctct gggctgtaca atcattgaaa     3240

tggccacagg aaaaccccca ttttatgaac tgggagaacc acaagcagct atgttcaagg     3300

tgggaatgtt taaagtccac cctgagatcc cagagtccat gtctgcagag gccaaggcat     3360

tcatactgaa atgttttgaa ccagatcctg acaagagagc ctgtgctaac gacttgcttg     3420

ttgatgagtt tttaaaagtt tcaagcaaaa agaaaaagac acaacctaag ctttcagctc     3480

tttcagctgg atcaaatgaa tatctcagga gtatatcctt gccggtacct gtgctggtgg     3540

aggacaccag cagcagcagt gagtacggct cagtttcacc cgacacggag ttgaaagtgg     3600

accccttctc tttcaaaaca agagccaagt cctgcggaga aagagatgtc aagggaattc     3660

ggacactctt tttgggcatt ccagatgaga attttgaaga tcacagtgct cctccttccc     3720

ctgaagaaaa agattctgga ttcttcatgc tgaggaagga cagtgagagg cgatctagat     3780

ctagagcggc cgcaggtacc tgaataacta aggccgcttc cctttagtga gggttaatgc     3840

ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt     3900

gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa     3960

gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg     4020

agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atccgataag     4080

gatcgatccg ggctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt     4140

gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg     4200

gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct     4260

ttcttccctt cctttcacgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg     4320

ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag     4380

ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg     4440

gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc     4500

tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat     4560

gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc     4620

tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgcggatctt     4680

ccgtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa     4740

gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac     4800

caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa     4860

ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag     4920

ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc     4980

cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt     5040

ttgcaaaaag cttgattctt ctgacacaac agtctcgaac ttaaggctag aattctggct     5100

acaggtaagc gcccctaaaa tccctttggg cacaatgtgt cctgagggga gaggcagcga     5160

cctgtagatg ggacgggggc actaaccctc aggtttgggg cttctgaatg tgagtatcgc     5220

catgtaagcc cagtatttgg ccaatctcag aaagctcctg gtccctggag ggatggagag     5280

agaaaaacaa acagctcctg gagcagggag agtgctggcc tcttgctctc cggctccctc     5340

tgttgccctc tggtttctcc ccaggctccc ggacgtcctc tagccaccat gacttcgaaa     5400

gtttatgatc cagaacaaag gaaacggatg ataactggtc cgcagtggtg ggccagatgt     5460

aaacaaatga atgttcttga ttcatttatt aattattatg attcagaaaa acatgcagaa     5520

aatgctgtta tttttttaca tggtaacgcg gcctcttctt atttatggcg acatgttgtg     5580

ccacatattg agccagtagc gcggtgtatt ataccagacc ttattggtat gggcaaatca     5640

ggcaaatctg gtaatggttc ttataggtta cttgatcatt acaaatatct tactgcatgg     5700

tttgaacttc ttaatttacc aaagaagatc atttttgtcg gccatgattg gggtgcttgt     5760

ttggcatttc attatagcta tgagcatcaa gataagatca aagcaatagt tcacgctgaa     5820

agtgtagtag atgtgattga atcatgggat gaatggcctg atattgaaga agatattgcg     5880

ttgatcaaat ctgaagaagg agaaaaaatg gttttggaga ataacttctt cgtggaaacc     5940

atgttgccat caaaaatcat gagaaagtta gaaccagaag aatttgcagc atatcttgaa     6000

ccattcaaag agaaaggtga agttcgtcgt ccaacattat catggcctcg tgaaatcccg     6060

ttagtaaaag gtggtaaacc tgacgttgta caaattgtta ggaattataa tgcttatcta     6120

cgtgcaagtg atgatttacc aaaaatgttt attgaatcgg acccaggatt cttttccaat     6180

gctattgttg aaggtgccaa gaagtttcct aatactgaat ttgtcaaagt aaaaggtctt     6240

catttttcgc aagaagatgc acctgatgaa atgggaaaat atatcaaatc gttcgttgag     6300

cgagttctca aaaatgaaca ataattctag ccctgaataa gtgataataa gcggatgaat     6360

ggcagaaatt cgtcgaagcg caataaaata tctttatttt cattacatct gtgtgttggt     6420

tttttgtgtg aatcgatagc gataaggatc ggaagatccg cgtatggtgc actctcagta     6480

caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg     6540

cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg     6600

ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc     6660

tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag     6720

gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt     6780

caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa     6840

ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt     6900

gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt     6960

tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt     7020

ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg     7080

tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga     7140

atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa     7200

gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga     7260

caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa     7320

ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca     7380

ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta     7440

ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac     7500

ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc     7560

gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag     7620

ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga     7680

taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt     7740

agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata     7800

atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag     7860

aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa     7920

caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt     7980

ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc     8040

cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa     8100

tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa     8160

gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc     8220

ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa     8280

gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa     8340

caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg     8400

ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc     8460

tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg     8520

ctcacatggc tcgacagatc t                                               8541


<210>  19
<211>  888
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gal4 ASK1(h) fusion protein sequence

<400>  19

Met Lys Leu Leu Ser Ser Ile Glu Gln Ala Cys Asp Ile Cys Arg Leu 
1               5                   10                  15      


Lys Lys Leu Lys Cys Ser Lys Glu Lys Pro Lys Cys Ala Lys Cys Leu 
            20                  25                  30          


Lys Asn Asn Trp Glu Cys Arg Tyr Ser Pro Lys Thr Lys Arg Ser Pro 
        35                  40                  45              


Leu Thr Arg Ala His Leu Thr Glu Val Glu Ser Arg Leu Glu Arg Leu 
    50                  55                  60                  


Glu Gln Leu Phe Leu Leu Ile Phe Pro Arg Glu Asp Leu Asp Met Ile 
65                  70                  75                  80  


Leu Lys Met Asp Ser Leu Gln Asp Ile Lys Ala Leu Leu Thr Gly Leu 
                85                  90                  95      


Phe Val Gln Asp Asn Val Asn Lys Asp Ala Val Thr Asp Arg Leu Ala 
            100                 105                 110         


Ser Val Glu Thr Asp Met Pro Leu Thr Leu Arg Gln His Arg Ile Ser 
        115                 120                 125             


Ala Thr Ser Ser Ser Glu Glu Ser Ser Asn Lys Gly Gln Arg Gln Leu 
    130                 135                 140                 


Thr Val Ser Pro Glu Phe Pro Gly Ile Arg Ser Tyr Arg Asp Ile Gln 
145                 150                 155                 160 


Asp Tyr Asp Ser Ile Val Lys Leu Val Glu Thr Leu Glu Lys Leu Pro 
                165                 170                 175     


Thr Phe Asp Leu Ala Ser His His His Val Lys Phe His Tyr Ala Phe 
            180                 185                 190         


Ala Leu Asn Arg Arg Asn Leu Pro Gly Asp Arg Ala Lys Ala Leu Asp 
        195                 200                 205             


Ile Met Ile Pro Met Val Gln Ser Glu Gly Gln Val Ala Ser Asp Met 
    210                 215                 220                 


Tyr Cys Leu Val Gly Arg Ile Tyr Lys Asp Met Phe Leu Asp Ser Asn 
225                 230                 235                 240 


Phe Thr Asp Thr Glu Ser Arg Asp His Gly Ala Ser Trp Phe Lys Lys 
                245                 250                 255     


Ala Phe Glu Ser Glu Pro Thr Leu Gln Ser Gly Ile Asn Tyr Ala Val 
            260                 265                 270         


Leu Leu Leu Ala Ala Gly His Gln Phe Glu Ser Ser Phe Glu Leu Arg 
        275                 280                 285             


Lys Val Gly Val Lys Leu Ser Ser Leu Leu Gly Lys Lys Gly Asn Leu 
    290                 295                 300                 


Glu Lys Leu Gln Ser Tyr Trp Glu Val Gly Phe Phe Leu Gly Ala Ser 
305                 310                 315                 320 


Val Leu Ala Asn Asp His Met Arg Val Ile Gln Ala Ser Glu Lys Leu 
                325                 330                 335     


Phe Lys Leu Lys Thr Pro Ala Trp Tyr Leu Lys Ser Ile Val Glu Thr 
            340                 345                 350         


Ile Leu Ile Tyr Lys His Phe Val Lys Leu Thr Thr Glu Gln Pro Val 
        355                 360                 365             


Ala Lys Gln Glu Leu Val Asp Phe Trp Met Asp Phe Leu Val Glu Ala 
    370                 375                 380                 


Thr Lys Thr Asp Val Thr Val Val Arg Phe Pro Val Leu Ile Leu Glu 
385                 390                 395                 400 


Pro Thr Lys Ile Tyr Gln Pro Ser Tyr Leu Ser Ile Asn Asn Glu Val 
                405                 410                 415     


Glu Glu Lys Thr Ile Ser Ile Trp His Val Leu Pro Asp Asp Lys Lys 
            420                 425                 430         


Gly Ile His Glu Trp Asn Phe Ser Ala Ser Ser Val Arg Gly Val Ser 
        435                 440                 445             


Ile Ser Lys Phe Glu Glu Arg Cys Cys Phe Leu Tyr Val Leu His Asn 
    450                 455                 460                 


Ser Asp Asp Phe Gln Ile Tyr Phe Cys Thr Glu Leu His Cys Lys Lys 
465                 470                 475                 480 


Phe Phe Glu Met Val Asn Thr Ile Thr Glu Glu Lys Gly Arg Ser Thr 
                485                 490                 495     


Glu Glu Gly Asp Cys Glu Ser Asp Leu Leu Glu Tyr Asp Tyr Glu Tyr 
            500                 505                 510         


Asp Glu Asn Gly Asp Arg Val Val Leu Gly Lys Gly Thr Tyr Gly Ile 
        515                 520                 525             


Val Tyr Ala Gly Arg Asp Leu Ser Asn Gln Val Arg Ile Ala Ile Lys 
    530                 535                 540                 


Glu Ile Pro Glu Arg Asp Ser Arg Tyr Ser Gln Pro Leu His Glu Glu 
545                 550                 555                 560 


Ile Ala Leu His Lys His Leu Lys His Lys Asn Ile Val Gln Tyr Leu 
                565                 570                 575     


Gly Ser Phe Ser Glu Asn Gly Phe Ile Lys Ile Phe Met Glu Gln Val 
            580                 585                 590         


Pro Gly Gly Ser Leu Ser Ala Leu Leu Arg Ser Lys Trp Gly Pro Leu 
        595                 600                 605             


Lys Asp Asn Glu Gln Thr Ile Gly Phe Tyr Thr Lys Gln Ile Leu Glu 
    610                 615                 620                 


Gly Leu Lys Tyr Leu His Asp Asn Gln Ile Val His Arg Asp Ile Lys 
625                 630                 635                 640 


Gly Asp Asn Val Leu Ile Asn Thr Tyr Ser Gly Val Leu Lys Ile Ser 
                645                 650                 655     


Asp Phe Gly Thr Ser Lys Arg Leu Ala Gly Ile Asn Pro Cys Thr Glu 
            660                 665                 670         


Thr Phe Thr Gly Thr Leu Gln Tyr Met Ala Pro Glu Ile Ile Asp Lys 
        675                 680                 685             


Gly Pro Arg Gly Tyr Gly Lys Ala Ala Asp Ile Trp Ser Leu Gly Cys 
    690                 695                 700                 


Thr Ile Ile Glu Met Ala Thr Gly Lys Pro Pro Phe Tyr Glu Leu Gly 
705                 710                 715                 720 


Glu Pro Gln Ala Ala Met Phe Lys Val Gly Met Phe Lys Val His Pro 
                725                 730                 735     


Glu Ile Pro Glu Ser Met Ser Ala Glu Ala Lys Ala Phe Ile Leu Lys 
            740                 745                 750         


Cys Phe Glu Pro Asp Pro Asp Lys Arg Ala Cys Ala Asn Asp Leu Leu 
        755                 760                 765             


Val Asp Glu Phe Leu Lys Val Ser Ser Lys Lys Lys Lys Thr Gln Pro 
    770                 775                 780                 


Lys Leu Ser Ala Leu Ser Ala Gly Ser Asn Glu Tyr Leu Arg Ser Ile 
785                 790                 795                 800 


Ser Leu Pro Val Pro Val Leu Val Glu Asp Thr Ser Ser Ser Ser Glu 
                805                 810                 815     


Tyr Gly Ser Val Ser Pro Asp Thr Glu Leu Lys Val Asp Pro Phe Ser 
            820                 825                 830         


Phe Lys Thr Arg Ala Lys Ser Cys Gly Glu Arg Asp Val Lys Gly Ile 
        835                 840                 845             


Arg Thr Leu Phe Leu Gly Ile Pro Asp Glu Asn Phe Glu Asp His Ser 
    850                 855                 860                 


Ala Pro Pro Ser Pro Glu Glu Lys Asp Ser Gly Phe Phe Met Leu Arg 
865                 870                 875                 880 


Lys Asp Ser Glu Arg Arg Ser Arg 
                885             


<210>  20
<211>  9046
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct sequence containing ASK1(i ) cloned in pBIND

<400>  20
tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta       60

ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc      120

aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg      180

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      240

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      300

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      360

ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga      420

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg      480

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac      540

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt      600

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg      660

cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata      720

agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac      780

agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt      840

gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa      900

ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact      960

cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac     1020

aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact     1080

ataggctagc cagcttgaag caagcctcct gaaagatgaa gctactgtct tctatcgaac     1140

aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg     1200

ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc     1260

tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc     1320

tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata     1380

taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag     1440

atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg     1500

cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgccgg     1560

aattcccggg gatccgtacg gaggcggacg agggcatcac tttctctgtg ccacccttcg     1620

ccccctcggg cttctgcacc atccccgagg gcggcatctg caggagggga ggagcggcgg     1680

cggtgggcga gggcgaggag caccagctgc caccgccgcc gccgggcagt ttctggaacg     1740

tggagagcgc cgctgcccct ggcatcggtt gtccggcggc cacctcctcg agcagtgcca     1800

cccgaggccg gggcagctct gttggcgggg gcagccgacg gaccacggtg gcatatgtga     1860

tcaacgaagc gagccaaggg caactggtgg tggccgagag cgaggccctg cagagcttgc     1920

gggaggcgtg cgagacagtg ggcgccaccc tggaaaccct gcattttggg aaactcgact     1980

ttggagaaac caccgtgctg gaccgctttt acaatgcaga tattgcggtg gtggagatga     2040

gcgatgcctt ccggcagccg tccttgtttt accaccttgg ggtgagagaa agtttcagca     2100

tggccaacaa catcatcctc tactgtgata ctaactcgga ctctctgcag tcactgaagg     2160

aaatcatttg ccagaagaat actatgtgca ctgggaacta cacctttgtt ccttacatga     2220

taactccaca taacaaagtc tactgctgtg acagcagctt catgaagggg ttgacagagc     2280

tcatgcaacc gaacttcgag ctgcttcttg gacccatctg cttacctctt gtggatcgtt     2340

ttattcaact tttgaaggtg gcacaagcaa gttctagcca gtacttccgg gaatctatac     2400

tcaatgacat caggaaagct cgtaatttat acactggtaa agaattggca gctgagttgg     2460

caagaattcg gcagcgagta gataatatcg aagtcttgac agcagatatt gtcataaatc     2520

tgttactttc ctacagagat atccaggacg tttatgattc tattgtgaag ctggtagaga     2580

ctttagaaaa actgccaacc tttgatttgg cctcccatca ccatgtgaag tttcattatg     2640

catttgcact gaataggaga aatctccctg gtgacagagc aaaagctctt gatattatga     2700

ttcccatggt gcaaagcgaa ggacaagttg cttcagatat gtattgccta gttggtcgaa     2760

tctacaaaga tatgtttttg gactctaatt tcacggacac tgaaagcaga gaccatggag     2820

cttcttggtt caaaaaggca tttgaatctg agccaacact acagtcagga attaattatg     2880

cggtcctcct cctggcagct ggacaccagt ttgaatcttc ctttgagctc cggaaagttg     2940

gggtgaagct aagtagtctt cttggtaaaa agggaaactt ggaaaaactc cagagctact     3000

gggaagttgg attttttctg ggggccagcg tcctagccaa tgaccacatg agagtcattc     3060

aagcatctga aaagcttttt aaactgaaga caccagcatg gtacctcaag tctattgtag     3120

agacaatttt aatatataag cattttgtga aactgaccac agaacagcct gtggccaagc     3180

aagaacttgt ggacttttgg atggatttcc tggtcgaggc cacaaagaca gatgttactg     3240

tggttaggtt tccagtatta atattagaac caaccaaaat ctatcaacct tcttatttgt     3300

ctatcaacaa tgaagttgag gaaaagacaa tctctatttg gcacgtgctt cctgatgaca     3360

agaaaggtat acatgagtgg aattttagtg cctcttctgt caggggagtg agtatttcta     3420

aatttgaaga aagatgctgc tttctttatg tgcttcacaa ttctgatgat ttccaaatct     3480

atttctgtac agaacttcat tgtaaaaagt tttttgagat ggtgaacacc attaccgaag     3540

agaaggggag aagcacagag gaaggagact gtgaaagtga cttgctggag tatgactatg     3600

aatatgatga aaatggtgac agagtcgttt taggaaaagg cacttatggg atagtctacg     3660

caggtcggga cttgagcaac caagtcagaa ttgctattaa ggaaatccca gagagagaca     3720

gcagatactc tcagcccctg catgaagaaa tagcattgca taaacacctg aagcacaaaa     3780

atattgtcca gtatctgggc tctttcagtg agaatggttt cattaaaatc ttcatggagc     3840

aggtccctgg aggaagtctt tctgctctcc ttcgttccaa atggggtcca ttaaaagaca     3900

atgagcaaac aattggcttt tatacaaagc aaatactgga aggattaaaa tatctccatg     3960

acaatcagat agttcaccgg gacataaagg gtgacaatgt gttgattaat acctacagtg     4020

gtgttctcaa gatctctgac ttcggaacat caaagaggct tgctggcata aacccctgta     4080

ctgaaacttt tactggtacc ctccagtata tggcaccaga aataatagat aaaggaccaa     4140

gaggctacgg aaaagcagca gacatctggt ctctgggctg tacaatcatt gaaatggcca     4200

caggaaaacc cccattttat gaactgggag aaccacaagc agctatgttc aaggtgggaa     4260

tgtttaaagt ccaccctgag atcctctaga gcggccgcag gtacctgaat aactaaggcc     4320

gcttcccttt agtgagggtt aatgcttcga gcagacatga taagatacat tgatgagttt     4380

ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct     4440

attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt     4500

cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc     4560

tacaaatgtg gtaaaatccg ataaggatcg atccgggctg gcgtaatagc gaagaggccc     4620

gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag     4680

cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag     4740

cgccctagcg cccgctcctt tcgctttctt cccttccttt cacgccacgt tcgccggctt     4800

tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca     4860

cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata     4920

gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca     4980

aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc     5040

gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa     5100

caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt     5160

atttcacacc gcatacgcgg atcttccgta ccttctgagg cggaaagaac cagctgtgga     5220

atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa     5280

gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca     5340

gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc     5400

ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt     5460

tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag     5520

gaggcttttt tggaggccta ggcttttgca aaaagcttga ttcttctgac acaacagtct     5580

cgaacttaag gctagaattc tggctacagg taagcgcccc taaaatccct ttgggcacaa     5640

tgtgtcctga ggggagaggc agcgacctgt agatgggacg ggggcactaa ccctcaggtt     5700

tggggcttct gaatgtgagt atcgccatgt aagcccagta tttggccaat ctcagaaagc     5760

tcctggtccc tggagggatg gagagagaaa aacaaacagc tcctggagca gggagagtgc     5820

tggcctcttg ctctccggct ccctctgttg ccctctggtt tctccccagg ctcccggacg     5880

tcctctagcc accatgactt cgaaagttta tgatccagaa caaaggaaac ggatgataac     5940

tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt cttgattcat ttattaatta     6000

ttatgattca gaaaaacatg cagaaaatgc tgttattttt ttacatggta acgcggcctc     6060

ttcttattta tggcgacatg ttgtgccaca tattgagcca gtagcgcggt gtattatacc     6120

agaccttatt ggtatgggca aatcaggcaa atctggtaat ggttcttata ggttacttga     6180

tcattacaaa tatcttactg catggtttga acttcttaat ttaccaaaga agatcatttt     6240

tgtcggccat gattggggtg cttgtttggc atttcattat agctatgagc atcaagataa     6300

gatcaaagca atagttcacg ctgaaagtgt agtagatgtg attgaatcat gggatgaatg     6360

gcctgatatt gaagaagata ttgcgttgat caaatctgaa gaaggagaaa aaatggtttt     6420

ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa atcatgagaa agttagaacc     6480

agaagaattt gcagcatatc ttgaaccatt caaagagaaa ggtgaagttc gtcgtccaac     6540

attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt aaacctgacg ttgtacaaat     6600

tgttaggaat tataatgctt atctacgtgc aagtgatgat ttaccaaaaa tgtttattga     6660

atcggaccca ggattctttt ccaatgctat tgttgaaggt gccaagaagt ttcctaatac     6720

tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa gatgcacctg atgaaatggg     6780

aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctagccctg     6840

aataagtgat aataagcgga tgaatggcag aaattcgtcg aagcgcaata aaatatcttt     6900

attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagcgataa ggatcggaag     6960

atccgcgtat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc     7020

cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct     7080

tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca     7140

ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg     7200

ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct     7260

atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga     7320

taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc     7380

cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg     7440

aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc     7500

aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact     7560

tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc     7620

ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag     7680

catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat     7740

aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt     7800

ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa     7860

gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc     7920

aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg     7980

gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt     8040

gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca     8100

gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat     8160

gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca     8220

gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg     8280

atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg     8340

ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt     8400

ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg     8460

ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata     8520

ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca     8580

ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag     8640

tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc     8700

tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga     8760

tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg     8820

tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac     8880

gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg     8940

tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg     9000

ttcctggcct tttgctggcc ttttgctcac atggctcgac agatct                    9046


<210>  21
<211>  1056
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gal4 ASK1(i) fusion protein sequence

<400>  21

Met Lys Leu Leu Ser Ser Ile Glu Gln Ala Cys Asp Ile Cys Arg Leu 
1               5                   10                  15      


Lys Lys Leu Lys Cys Ser Lys Glu Lys Pro Lys Cys Ala Lys Cys Leu 
            20                  25                  30          


Lys Asn Asn Trp Glu Cys Arg Tyr Ser Pro Lys Thr Lys Arg Ser Pro 
        35                  40                  45              


Leu Thr Arg Ala His Leu Thr Glu Val Glu Ser Arg Leu Glu Arg Leu 
    50                  55                  60                  


Glu Gln Leu Phe Leu Leu Ile Phe Pro Arg Glu Asp Leu Asp Met Ile 
65                  70                  75                  80  


Leu Lys Met Asp Ser Leu Gln Asp Ile Lys Ala Leu Leu Thr Gly Leu 
                85                  90                  95      


Phe Val Gln Asp Asn Val Asn Lys Asp Ala Val Thr Asp Arg Leu Ala 
            100                 105                 110         


Ser Val Glu Thr Asp Met Pro Leu Thr Leu Arg Gln His Arg Ile Ser 
        115                 120                 125             


Ala Thr Ser Ser Ser Glu Glu Ser Ser Asn Lys Gly Gln Arg Gln Leu 
    130                 135                 140                 


Thr Val Ser Pro Glu Phe Pro Gly Ile Arg Thr Glu Ala Asp Glu Gly 
145                 150                 155                 160 


Ile Thr Phe Ser Val Pro Pro Phe Ala Pro Ser Gly Phe Cys Thr Ile 
                165                 170                 175     


Pro Glu Gly Gly Ile Cys Arg Arg Gly Gly Ala Ala Ala Val Gly Glu 
            180                 185                 190         


Gly Glu Glu His Gln Leu Pro Pro Pro Pro Pro Gly Ser Phe Trp Asn 
        195                 200                 205             


Val Glu Ser Ala Ala Ala Pro Gly Ile Gly Cys Pro Ala Ala Thr Ser 
    210                 215                 220                 


Ser Ser Ser Ala Thr Arg Gly Arg Gly Ser Ser Val Gly Gly Gly Ser 
225                 230                 235                 240 


Arg Arg Thr Thr Val Ala Tyr Val Ile Asn Glu Ala Ser Gln Gly Gln 
                245                 250                 255     


Leu Val Val Ala Glu Ser Glu Ala Leu Gln Ser Leu Arg Glu Ala Cys 
            260                 265                 270         


Glu Thr Val Gly Ala Thr Leu Glu Thr Leu His Phe Gly Lys Leu Asp 
        275                 280                 285             


Phe Gly Glu Thr Thr Val Leu Asp Arg Phe Tyr Asn Ala Asp Ile Ala 
    290                 295                 300                 


Val Val Glu Met Ser Asp Ala Phe Arg Gln Pro Ser Leu Phe Tyr His 
305                 310                 315                 320 


Leu Gly Val Arg Glu Ser Phe Ser Met Ala Asn Asn Ile Ile Leu Tyr 
                325                 330                 335     


Cys Asp Thr Asn Ser Asp Ser Leu Gln Ser Leu Lys Glu Ile Ile Cys 
            340                 345                 350         


Gln Lys Asn Thr Met Cys Thr Gly Asn Tyr Thr Phe Val Pro Tyr Met 
        355                 360                 365             


Ile Thr Pro His Asn Lys Val Tyr Cys Cys Asp Ser Ser Phe Met Lys 
    370                 375                 380                 


Gly Leu Thr Glu Leu Met Gln Pro Asn Phe Glu Leu Leu Leu Gly Pro 
385                 390                 395                 400 


Ile Cys Leu Pro Leu Val Asp Arg Phe Ile Gln Leu Leu Lys Val Ala 
                405                 410                 415     


Gln Ala Ser Ser Ser Gln Tyr Phe Arg Glu Ser Ile Leu Asn Asp Ile 
            420                 425                 430         


Arg Lys Ala Arg Asn Leu Tyr Thr Gly Lys Glu Leu Ala Ala Glu Leu 
        435                 440                 445             


Ala Arg Ile Arg Gln Arg Val Asp Asn Ile Glu Val Leu Thr Ala Asp 
    450                 455                 460                 


Ile Val Ile Asn Leu Leu Leu Ser Tyr Arg Asp Ile Gln Asp Val Tyr 
465                 470                 475                 480 


Asp Ser Ile Val Lys Leu Val Glu Thr Leu Glu Lys Leu Pro Thr Phe 
                485                 490                 495     


Asp Leu Ala Ser His His His Val Lys Phe His Tyr Ala Phe Ala Leu 
            500                 505                 510         


Asn Arg Arg Asn Leu Pro Gly Asp Arg Ala Lys Ala Leu Asp Ile Met 
        515                 520                 525             


Ile Pro Met Val Gln Ser Glu Gly Gln Val Ala Ser Asp Met Tyr Cys 
    530                 535                 540                 


Leu Val Gly Arg Ile Tyr Lys Asp Met Phe Leu Asp Ser Asn Phe Thr 
545                 550                 555                 560 


Asp Thr Glu Ser Arg Asp His Gly Ala Ser Trp Phe Lys Lys Ala Phe 
                565                 570                 575     


Glu Ser Glu Pro Thr Leu Gln Ser Gly Ile Asn Tyr Ala Val Leu Leu 
            580                 585                 590         


Leu Ala Ala Gly His Gln Phe Glu Ser Ser Phe Glu Leu Arg Lys Val 
        595                 600                 605             


Gly Val Lys Leu Ser Ser Leu Leu Gly Lys Lys Gly Asn Leu Glu Lys 
    610                 615                 620                 


Leu Gln Ser Tyr Trp Glu Val Gly Phe Phe Leu Gly Ala Ser Val Leu 
625                 630                 635                 640 


Ala Asn Asp His Met Arg Val Ile Gln Ala Ser Glu Lys Leu Phe Lys 
                645                 650                 655     


Leu Lys Thr Pro Ala Trp Tyr Leu Lys Ser Ile Val Glu Thr Ile Leu 
            660                 665                 670         


Ile Tyr Lys His Phe Val Lys Leu Thr Thr Glu Gln Pro Val Ala Lys 
        675                 680                 685             


Gln Glu Leu Val Asp Phe Trp Met Asp Phe Leu Val Glu Ala Thr Lys 
    690                 695                 700                 


Thr Asp Val Thr Val Val Arg Phe Pro Val Leu Ile Leu Glu Pro Thr 
705                 710                 715                 720 


Lys Ile Tyr Gln Pro Ser Tyr Leu Ser Ile Asn Asn Glu Val Glu Glu 
                725                 730                 735     


Lys Thr Ile Ser Ile Trp His Val Leu Pro Asp Asp Lys Lys Gly Ile 
            740                 745                 750         


His Glu Trp Asn Phe Ser Ala Ser Ser Val Arg Gly Val Ser Ile Ser 
        755                 760                 765             


Lys Phe Glu Glu Arg Cys Cys Phe Leu Tyr Val Leu His Asn Ser Asp 
    770                 775                 780                 


Asp Phe Gln Ile Tyr Phe Cys Thr Glu Leu His Cys Lys Lys Phe Phe 
785                 790                 795                 800 


Glu Met Val Asn Thr Ile Thr Glu Glu Lys Gly Arg Ser Thr Glu Glu 
                805                 810                 815     


Gly Asp Cys Glu Ser Asp Leu Leu Glu Tyr Asp Tyr Glu Tyr Asp Glu 
            820                 825                 830         


Asn Gly Asp Arg Val Val Leu Gly Lys Gly Thr Tyr Gly Ile Val Tyr 
        835                 840                 845             


Ala Gly Arg Asp Leu Ser Asn Gln Val Arg Ile Ala Ile Lys Glu Ile 
    850                 855                 860                 


Pro Glu Arg Asp Ser Arg Tyr Ser Gln Pro Leu His Glu Glu Ile Ala 
865                 870                 875                 880 


Leu His Lys His Leu Lys His Lys Asn Ile Val Gln Tyr Leu Gly Ser 
                885                 890                 895     


Phe Ser Glu Asn Gly Phe Ile Lys Ile Phe Met Glu Gln Val Pro Gly 
            900                 905                 910         


Gly Ser Leu Ser Ala Leu Leu Arg Ser Lys Trp Gly Pro Leu Lys Asp 
        915                 920                 925             


Asn Glu Gln Thr Ile Gly Phe Tyr Thr Lys Gln Ile Leu Glu Gly Leu 
    930                 935                 940                 


Lys Tyr Leu His Asp Asn Gln Ile Val His Arg Asp Ile Lys Gly Asp 
945                 950                 955                 960 


Asn Val Leu Ile Asn Thr Tyr Ser Gly Val Leu Lys Ile Ser Asp Phe 
                965                 970                 975     


Gly Thr Ser Lys Arg Leu Ala Gly Ile Asn Pro Cys Thr Glu Thr Phe 
            980                 985                 990         


Thr Gly Thr Leu Gln Tyr Met Ala  Pro Glu Ile Ile Asp  Lys Gly Pro 
        995                 1000                 1005             


Arg Gly  Tyr Gly Lys Ala Ala  Asp Ile Trp Ser Leu  Gly Cys Thr 
    1010                 1015                 1020             


Ile Ile  Glu Met Ala Thr Gly  Lys Pro Pro Phe Tyr  Glu Leu Gly 
    1025                 1030                 1035             


Glu Pro  Gln Ala Ala Met Phe  Lys Val Gly Met Phe  Lys Val His 
    1040                 1045                 1050             


Pro Glu  Ile 
    1055     


<210>  22
<211>  8104
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct sequence containing ASK1(j) cloned in pBIND

<400>  22
tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta       60

ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc      120

aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg      180

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc      240

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat      300

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc      360

ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga      420

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg      480

gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac      540

caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt      600

caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg      660

cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata      720

agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac      780

agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt      840

gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa      900

ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact      960

cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac     1020

aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact     1080

ataggctagc cagcttgaag caagcctcct gaaagatgaa gctactgtct tctatcgaac     1140

aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg     1200

ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc     1260

tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc     1320

tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata     1380

taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag     1440

atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg     1500

cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgccgg     1560

aattcccggg gatccggatc cgtctttcct acagagatat ccaggacgtt tatgattcta     1620

ttgtgaagct ggtagagact ttagaaaaac tgccaacctt tgatttggcc tcccatcacc     1680

atgtgaagtt tcattatgca tttgcactga ataggagaaa tctccctggt gacagagcaa     1740

aagctcttga tattatgatt cccatggtgc aaagcgaagg acaagttgct tcagatatgt     1800

attgcctagt tggtcgaatc tacaaagata tgtttttgga ctctaatttc acggacactg     1860

aaagcagaga ccatggagct tcttggttca aaaaggcatt tgaatctgag ccaacactac     1920

agtcaggaat taattatgcg gtcctcctcc tggcagctgg acaccagttt gaatcttcct     1980

ttgagctccg gaaagttggg gtgaagctaa gtagtcttct tggtaaaaag ggaaacttgg     2040

aaaaactcca gagctactgg gaagttggat tttttctggg ggccagcgtc ctagccaatg     2100

accacatgag agtcattcaa gcatctgaaa agctttttaa actgaagaca ccagcatggt     2160

acctcaagtc tattgtagag acaattttaa tatataagca ttttgtgaaa ctgaccacag     2220

aacagcctgt ggccaagcaa gaacttgtgg acttttggat ggatttcctg gtcgaggcca     2280

caaagacaga tgttactgtg gttaggtttc cagtattaat attagaacca accaaaatct     2340

atcaaccttc ttatttgtct atcaacaatg aagttgagga aaagacaatc tctatttggc     2400

acgtgcttcc tgatgacaag aaaggtatac atgagtggaa ttttagtgcc tcttctgtca     2460

ggggagtgag tatttctaaa tttgaagaaa gatgctgctt tctttatgtg cttcacaatt     2520

ctgatgattt ccaaatctat ttctgtacag aacttcattg taaaaagttt tttgagatgg     2580

tgaacaccat taccgaagag aaggggagaa gcacagagga aggagactgt gaaagtgact     2640

tgctggagta tgactatgaa tatgatgaaa atggtgacag agtcgtttta ggaaaaggca     2700

cttatgggat agtctacgca ggtcgggact tgagcaacca agtcagaatt gctattaagg     2760

aaatcccaga gagagacagc agatactctc agcccctgca tgaagaaata gcattgcata     2820

aacacctgaa gcacaaaaat attgtccagt atctgggctc tttcagtgag aatggtttca     2880

ttaaaatctt catggagcag gtccctggag gaagtctttc tgctctcctt cgttccaaat     2940

ggggtccatt aaaagacaat gagcaaacaa ttggctttta tacaaagcaa atactggaag     3000

gattaaaata tctccatgac aatcagatag ttcaccggga cataaagggt gacaatgtgt     3060

tgattaatac ctacagtggt gttctcaaga tctctgactt cggaacatca aagaggcttg     3120

ctggcataaa cccctgtact gaaactttta ctggtaccct ccagtatatg gcaccagaaa     3180

taatagataa aggaccaaga ggctacggaa aagcagcaga catctggtct ctgggctgta     3240

caatcattga aatggccaca ggaaaacccc cattttatga actgggagaa ccacaagcag     3300

ctatgttcaa ggtgggaatg tttaaagtcc accctgagat cctctagagc ggccgcaggt     3360

acctgaataa ctaaggccgc ttccctttag tgagggttaa tgcttcgagc agacatgata     3420

agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt     3480

tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt     3540

aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt     3600

taaagcaagt aaaacctcta caaatgtggt aaaatccgat aaggatcgat ccgggctggc     3660

gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg     3720

aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt     3780

gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttca     3840

cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg     3900

atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag     3960

tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa     4020

tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga     4080

tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa     4140

atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc     4200

cttacgcatc tgtgcggtat ttcacaccgc atacgcggat cttccgtacc ttctgaggcg     4260

gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag     4320

caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc     4380

caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag     4440

tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc     4500

cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc     4560

tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttgatt     4620

cttctgacac aacagtctcg aacttaaggc tagaattctg gctacaggta agcgccccta     4680

aaatcccttt gggcacaatg tgtcctgagg ggagaggcag cgacctgtag atgggacggg     4740

ggcactaacc ctcaggtttg gggcttctga atgtgagtat cgccatgtaa gcccagtatt     4800

tggccaatct cagaaagctc ctggtccctg gagggatgga gagagaaaaa caaacagctc     4860

ctggagcagg gagagtgctg gcctcttgct ctccggctcc ctctgttgcc ctctggtttc     4920

tccccaggct cccggacgtc ctctagccac catgacttcg aaagtttatg atccagaaca     4980

aaggaaacgg atgataactg gtccgcagtg gtgggccaga tgtaaacaaa tgaatgttct     5040

tgattcattt attaattatt atgattcaga aaaacatgca gaaaatgctg ttattttttt     5100

acatggtaac gcggcctctt cttatttatg gcgacatgtt gtgccacata ttgagccagt     5160

agcgcggtgt attataccag accttattgg tatgggcaaa tcaggcaaat ctggtaatgg     5220

ttcttatagg ttacttgatc attacaaata tcttactgca tggtttgaac ttcttaattt     5280

accaaagaag atcatttttg tcggccatga ttggggtgct tgtttggcat ttcattatag     5340

ctatgagcat caagataaga tcaaagcaat agttcacgct gaaagtgtag tagatgtgat     5400

tgaatcatgg gatgaatggc ctgatattga agaagatatt gcgttgatca aatctgaaga     5460

aggagaaaaa atggttttgg agaataactt cttcgtggaa accatgttgc catcaaaaat     5520

catgagaaag ttagaaccag aagaatttgc agcatatctt gaaccattca aagagaaagg     5580

tgaagttcgt cgtccaacat tatcatggcc tcgtgaaatc ccgttagtaa aaggtggtaa     5640

acctgacgtt gtacaaattg ttaggaatta taatgcttat ctacgtgcaa gtgatgattt     5700

accaaaaatg tttattgaat cggacccagg attcttttcc aatgctattg ttgaaggtgc     5760

caagaagttt cctaatactg aatttgtcaa agtaaaaggt cttcattttt cgcaagaaga     5820

tgcacctgat gaaatgggaa aatatatcaa atcgttcgtt gagcgagttc tcaaaaatga     5880

acaataattc tagccctgaa taagtgataa taagcggatg aatggcagaa attcgtcgaa     5940

gcgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat     6000

agcgataagg atcggaagat ccgcgtatgg tgcactctca gtacaatctg ctctgatgcc     6060

gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt     6120

ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag     6180

aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt     6240

ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga     6300

aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc     6360

atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt     6420

caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct     6480

cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt     6540

tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt     6600

tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac     6660

gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac     6720

tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct     6780

gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg     6840

aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg     6900

gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca     6960

atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa     7020

caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt     7080

ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc     7140

attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg     7200

agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt     7260

aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt     7320

catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc     7380

ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct     7440

tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta     7500

ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc     7560

ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac     7620

ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct     7680

gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat     7740

aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg     7800

acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa     7860

gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg     7920

gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga     7980

cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc     8040

aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat ggctcgacag     8100

atct                                                                  8104


<210>  23
<211>  742
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gal4 ASK1(j) fusion protein sequence

<400>  23

Met Lys Leu Leu Ser Ser Ile Glu Gln Ala Cys Asp Ile Cys Arg Leu 
1               5                   10                  15      


Lys Lys Leu Lys Cys Ser Lys Glu Lys Pro Lys Cys Ala Lys Cys Leu 
            20                  25                  30          


Lys Asn Asn Trp Glu Cys Arg Tyr Ser Pro Lys Thr Lys Arg Ser Pro 
        35                  40                  45              


Leu Thr Arg Ala His Leu Thr Glu Val Glu Ser Arg Leu Glu Arg Leu 
    50                  55                  60                  


Glu Gln Leu Phe Leu Leu Ile Phe Pro Arg Glu Asp Leu Asp Met Ile 
65                  70                  75                  80  


Leu Lys Met Asp Ser Leu Gln Asp Ile Lys Ala Leu Leu Thr Gly Leu 
                85                  90                  95      


Phe Val Gln Asp Asn Val Asn Lys Asp Ala Val Thr Asp Arg Leu Ala 
            100                 105                 110         


Ser Val Glu Thr Asp Met Pro Leu Thr Leu Arg Gln His Arg Ile Ser 
        115                 120                 125             


Ala Thr Ser Ser Ser Glu Glu Ser Ser Asn Lys Gly Gln Arg Gln Leu 
    130                 135                 140                 


Thr Val Ser Pro Glu Phe Pro Gly Ile Arg Ile Arg Leu Ser Tyr Arg 
145                 150                 155                 160 


Asp Ile Gln Asp Val Tyr Asp Ser Ile Val Lys Leu Val Glu Thr Leu 
                165                 170                 175     


Glu Lys Leu Pro Thr Phe Asp Leu Ala Ser His His His Val Lys Phe 
            180                 185                 190         


His Tyr Ala Phe Ala Leu Asn Arg Arg Asn Leu Pro Gly Asp Arg Ala 
        195                 200                 205             


Lys Ala Leu Asp Ile Met Ile Pro Met Val Gln Ser Glu Gly Gln Val 
    210                 215                 220                 


Ala Ser Asp Met Tyr Cys Leu Val Gly Arg Ile Tyr Lys Asp Met Phe 
225                 230                 235                 240 


Leu Asp Ser Asn Phe Thr Asp Thr Glu Ser Arg Asp His Gly Ala Ser 
                245                 250                 255     


Trp Phe Lys Lys Ala Phe Glu Ser Glu Pro Thr Leu Gln Ser Gly Ile 
            260                 265                 270         


Asn Tyr Ala Val Leu Leu Leu Ala Ala Gly His Gln Phe Glu Ser Ser 
        275                 280                 285             


Phe Glu Leu Arg Lys Val Gly Val Lys Leu Ser Ser Leu Leu Gly Lys 
    290                 295                 300                 


Lys Gly Asn Leu Glu Lys Leu Gln Ser Tyr Trp Glu Val Gly Phe Phe 
305                 310                 315                 320 


Leu Gly Ala Ser Val Leu Ala Asn Asp His Met Arg Val Ile Gln Ala 
                325                 330                 335     


Ser Glu Lys Leu Phe Lys Leu Lys Thr Pro Ala Trp Tyr Leu Lys Ser 
            340                 345                 350         


Ile Val Glu Thr Ile Leu Ile Tyr Lys His Phe Val Lys Leu Thr Thr 
        355                 360                 365             


Glu Gln Pro Val Ala Lys Gln Glu Leu Val Asp Phe Trp Met Asp Phe 
    370                 375                 380                 


Leu Val Glu Ala Thr Lys Thr Asp Val Thr Val Val Arg Phe Pro Val 
385                 390                 395                 400 


Leu Ile Leu Glu Pro Thr Lys Ile Tyr Gln Pro Ser Tyr Leu Ser Ile 
                405                 410                 415     


Asn Asn Glu Val Glu Glu Lys Thr Ile Ser Ile Trp His Val Leu Pro 
            420                 425                 430         


Asp Asp Lys Lys Gly Ile His Glu Trp Asn Phe Ser Ala Ser Ser Val 
        435                 440                 445             


Arg Gly Val Ser Ile Ser Lys Phe Glu Glu Arg Cys Cys Phe Leu Tyr 
    450                 455                 460                 


Val Leu His Asn Ser Asp Asp Phe Gln Ile Tyr Phe Cys Thr Glu Leu 
465                 470                 475                 480 


His Cys Lys Lys Phe Phe Glu Met Val Asn Thr Ile Thr Glu Glu Lys 
                485                 490                 495     


Gly Arg Ser Thr Glu Glu Gly Asp Cys Glu Ser Asp Leu Leu Glu Tyr 
            500                 505                 510         


Asp Tyr Glu Tyr Asp Glu Asn Gly Asp Arg Val Val Leu Gly Lys Gly 
        515                 520                 525             


Thr Tyr Gly Ile Val Tyr Ala Gly Arg Asp Leu Ser Asn Gln Val Arg 
    530                 535                 540                 


Ile Ala Ile Lys Glu Ile Pro Glu Arg Asp Ser Arg Tyr Ser Gln Pro 
545                 550                 555                 560 


Leu His Glu Glu Ile Ala Leu His Lys His Leu Lys His Lys Asn Ile 
                565                 570                 575     


Val Gln Tyr Leu Gly Ser Phe Ser Glu Asn Gly Phe Ile Lys Ile Phe 
            580                 585                 590         


Met Glu Gln Val Pro Gly Gly Ser Leu Ser Ala Leu Leu Arg Ser Lys 
        595                 600                 605             


Trp Gly Pro Leu Lys Asp Asn Glu Gln Thr Ile Gly Phe Tyr Thr Lys 
    610                 615                 620                 


Gln Ile Leu Glu Gly Leu Lys Tyr Leu His Asp Asn Gln Ile Val His 
625                 630                 635                 640 


Arg Asp Ile Lys Gly Asp Asn Val Leu Ile Asn Thr Tyr Ser Gly Val 
                645                 650                 655     


Leu Lys Ile Ser Asp Phe Gly Thr Ser Lys Arg Leu Ala Gly Ile Asn 
            660                 665                 670         


Pro Cys Thr Glu Thr Phe Thr Gly Thr Leu Gln Tyr Met Ala Pro Glu 
        675                 680                 685             


Ile Ile Asp Lys Gly Pro Arg Gly Tyr Gly Lys Ala Ala Asp Ile Trp 
    690                 695                 700                 


Ser Leu Gly Cys Thr Ile Ile Glu Met Ala Thr Gly Lys Pro Pro Phe 
705                 710                 715                 720 


Tyr Glu Leu Gly Glu Pro Gln Ala Ala Met Phe Lys Val Gly Met Phe 
                725                 730                 735     


Lys Val His Pro Glu Ile 
            740         


<210>  24
<211>  4733
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pEYFP-N1 Sequence

<400>  24
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg      660

gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc      720

atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc      780

gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg      840

cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc      900

taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc      960

caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag     1020

ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac     1080

ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg     1140

gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac     1200

ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg     1260

ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag     1320

aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg     1380

gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg     1440

tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa     1500

tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca     1560

atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt     1620

ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc     1680

gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc     1740

ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag     1800

agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc     1860

gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa     1920

gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg     1980

aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt     2040

gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc     2100

gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa     2160

atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat     2220

tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt     2280

gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt     2340

cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc     2400

atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc     2460

cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg     2520

ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc     2580

taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa     2640

gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg     2700

gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc     2760

ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca     2820

gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc     2880

actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca     2940

tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat     3000

acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca     3060

cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg     3120

ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc     3180

gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct     3240

ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct     3300

acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac     3360

ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc     3420

tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag     3480

atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg     3540

ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg     3600

ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa     3660

taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt     3720

cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg     3780

tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca     3840

acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt     3900

taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga     3960

ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca     4020

aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac     4080

caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg     4140

taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag     4200

gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac     4260

cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt     4320

taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg     4380

agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc     4440

ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc     4500

gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc     4560

acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa     4620

acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt     4680

tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cat            4733


<210>  25
<211>  5345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 Nef Sequence cloned in pEYFP N1

<400>  25
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggactcag atctcgagct caagcttatg gggggcaagt ggtcaaaaag cagtatagtt      660

ggatggcctg cagtgagaga aagaataaga agaactgagc cagcagcaga gggagtagga      720

gcagcgtctc gagacttaga taaacatgga gcacttacaa gcagcaacac agcccaaact      780

aatgctgatt gtgcctggct ggaagcacaa gaggaggaaa acgaagtagg gtttccagtc      840

agacctcagg tgccgctcag accaatgact tataaggggg cagtagatct cagcttcttt      900

ttaaaagaaa aggggggact ggaagggtta acttactcta agaaaagaca agaaatcctt      960

gatttgtggg tctatcacac acaaggctac ttccctgatt ggcaatgcta cacaccggga     1020

ccaggggtca gatacccact gacttttgga tggtgcttca agctagtgcc agttgaccca     1080

gatgaagtag gagaagccaa caatggagag aataactgtt tgctacaccc tataagcctg     1140

catggaatgg aggatccaga aagagaagta ttaaggtgga agtttgacag tcacctagca     1200

cacacacaca aggcccgcga gctacatccg gagtattaca aagactgcgc gtcgacggta     1260

ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc     1320

ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg     1380

tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc     1440

accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag     1500

tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc     1560

gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc     1620

gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac     1680

ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac     1740

gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac     1800

aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc     1860

gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa     1920

gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc     1980

actctcggca tggacgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc     2040

ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc     2100

tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt     2160

acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta     2220

gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt     2280

ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa     2340

atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca     2400

gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc     2460

gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg     2520

aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg     2580

ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg     2640

gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg     2700

ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt     2760

ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg     2820

cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt     2880

gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc     2940

atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta     3000

tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc     3060

cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta     3120

tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct     3180

tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc     3240

atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc     3300

ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca     3360

gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg     3420

caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg     3480

ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag     3540

gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg     3600

cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc     3660

atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa     3720

gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac     3780

ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat     3840

ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac     3900

atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc     3960

ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt     4020

gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc     4080

tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg     4140

ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg     4200

cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg     4260

ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac     4320

gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc     4380

aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag     4440

ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac     4500

tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg     4560

ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg     4620

tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc     4680

aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc     4740

tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt     4800

agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc     4860

taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact     4920

caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac     4980

agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag     5040

aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg     5100

gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg     5160

tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga     5220

gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt     5280

ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca     5340

tgcat                                                                 5345


<210>  26
<211>  460
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HIV-1 Nef YFP fusion protein Sequence

<400>  26

Met Gly Gly Lys Trp Ser Lys Ser Ser Ile Val Gly Trp Pro Ala Val 
1               5                   10                  15      


Arg Glu Arg Ile Arg Arg Thr Glu Pro Ala Ala Glu Gly Val Gly Ala 
            20                  25                  30          


Ala Ser Arg Asp Leu Asp Lys His Gly Ala Leu Thr Ser Ser Asn Thr 
        35                  40                  45              


Ala Gln Thr Asn Ala Asp Cys Ala Trp Leu Glu Ala Gln Glu Glu Glu 
    50                  55                  60                  


Asn Glu Val Gly Phe Pro Val Arg Pro Gln Val Pro Leu Arg Pro Met 
65                  70                  75                  80  


Thr Tyr Lys Gly Ala Val Asp Leu Ser Phe Phe Leu Lys Glu Lys Gly 
                85                  90                  95      


Gly Leu Glu Gly Leu Thr Tyr Ser Lys Lys Arg Gln Glu Ile Leu Asp 
            100                 105                 110         


Leu Trp Val Tyr His Thr Gln Gly Tyr Phe Pro Asp Trp Gln Cys Tyr 
        115                 120                 125             


Thr Pro Gly Pro Gly Val Arg Tyr Pro Leu Thr Phe Gly Trp Cys Phe 
    130                 135                 140                 


Lys Leu Val Pro Val Asp Pro Asp Glu Val Gly Glu Ala Asn Asn Gly 
145                 150                 155                 160 


Glu Asn Asn Cys Leu Leu His Pro Ile Ser Leu His Gly Met Glu Asp 
                165                 170                 175     


Pro Glu Arg Glu Val Leu Arg Trp Lys Phe Asp Ser His Leu Ala His 
            180                 185                 190         


Thr His Lys Ala Arg Glu Leu His Pro Glu Tyr Tyr Lys Asp Cys Ala 
        195                 200                 205             


Ser Thr Val Pro Arg Ala Arg Asp Pro Pro Val Ala Thr Met Val Ser 
    210                 215                 220                 


Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu 
225                 230                 235                 240 


Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu 
                245                 250                 255     


Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr 
            260                 265                 270         


Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe Gly Tyr 
        275                 280                 285             


Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln His Asp 
    290                 295                 300                 


Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile 
305                 310                 315                 320 


Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe 
                325                 330                 335     


Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe 
            340                 345                 350         


Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn 
        355                 360                 365             


Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys 
    370                 375                 380                 


Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu 
385                 390                 395                 400 


Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 
                405                 410                 415     


Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu Ser Lys Asp 
            420                 425                 430         


Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala 
        435                 440                 445             


Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
    450                 455                 460 


<210>  27
<211>  7870
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct Sequence containing ASK (g) cloned in pEYFP

<400>  27
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggactcag atctcgagct caagcttcga attctgcagt cgacatgagc acggaggcgg      660

acgagggcat cactttctct gtgccaccct tcgccccctc gggcttctgc accatccccg      720

agggcggcat ctgcaggagg ggaggagcgg cggcggtggg cgagggcgag gagcaccagc      780

tgccaccgcc gccgccgggc agtttctgga acgtggagag cgccgctgcc cctggcatcg      840

gttgtccggc ggccacctcc tcgagcagtg ccacccgagg ccggggcagc tctgttggcg      900

ggggcagccg acggaccacg gtggcatatg tgatcaacga agcgagccaa gggcaactgg      960

tggtggccga gagcgaggcc ctgcagagct tgcgggaggc gtgcgagaca gtgggcgcca     1020

ccctggaaac cctgcatttt gggaaactcg actttggaga aaccaccgtg ctggaccgct     1080

tttacaatgc agatattgcg gtggtggaga tgagcgatgc cttccggcag ccgtccttgt     1140

tttaccacct tggggtgaga gaaagtttca gcatggccaa caacatcatc ctctactgtg     1200

atactaactc ggactctctg cagtcactga aggaaatcat ttgccagaag aatactatgt     1260

gcactgggaa ctacaccttt gttccttaca tgataactcc acataacaaa gtctactgct     1320

gtgacagcag cttcatgaag gggttgacag agctcatgca accgaacttc gagctgcttc     1380

ttggacccat ctgcttacct cttgtggatc gttttattca acttttgaag gtggcacaag     1440

caagttctag ccagtacttc cgggaatcta tactcaatga catcaggaaa gctcgtaatt     1500

tatacactgg taaagaattg gcagctgagt tggcaagaat tcggcagcga gtagataata     1560

tcgaagtctt gacagcagat attgtcataa atctgttact ttcctacaga gatatccagg     1620

actatgattc tattgtgaag ctggtagaga ctttagaaaa actgccaacc tttgatttgg     1680

cctcccatca ccatgtgaag tttcattatg catttgcact gaataggaga aatctccctg     1740

gtgacagagc aaaagctctt gatattatga ttcccatggt gcaaagcgaa ggacaagttg     1800

cttcagatat gtattgccta gttggtcgaa tctacaaaga tatgtttttg gactctaatt     1860

tcacggacac tgaaagcaga gaccatggag cttcttggtt caaaaaggca tttgaatctg     1920

agccaacact acagtcagga attaattatg cggtcctcct cctggcagct ggacaccagt     1980

ttgaatcttc ctttgagctc cggaaagttg gggtgaagct aagtagtctt cttggtaaaa     2040

agggaaactt ggaaaaactc cagagctact gggaagttgg attttttctg ggggccagcg     2100

tcctagccaa tgaccacatg agagtcattc aagcatctga aaagcttttt aaactgaaga     2160

caccagcatg gtacctcaag tctattgtag agacaatttt aatatataag cattttgtga     2220

aactgaccac agaacagcct gtggccaagc aagaacttgt ggacttttgg atggatttcc     2280

tggtcgaggc cacaaagaca gatgttactg tggttaggtt tccagtatta atattagaac     2340

caaccaaaat ctatcaacct tcttatttgt ctatcaacaa tgaagttgag gaaaagacaa     2400

tctctatttg gcacgtgctt cctgatgaca agaaaggtat acatgagtgg aattttagtg     2460

cctcttctgt caggggagtg agtatttcta aatttgaaga aagatgctgc tttctttatg     2520

tgcttcacaa ttctgatgat ttccaaatct atttctgtac agaacttcat tgtaaaaagt     2580

tttttgagat ggtgaacacc attaccgaag agaaggggag aagcacagag gaaggagact     2640

gtgaaagtga cttgctggag tatgactatg aatatgatga aaatggtgac agagtcgttt     2700

taggaaaagg cacttatggg atagtctacg caggtcggga cttgagcaac caagtcagaa     2760

ttgctattaa ggaaatccca gagagagaca gcagatactc tcagcccctg catgaagaaa     2820

tagcattgca taaacacctg aagcacaaaa atattgtcca gtatctgggc tctttcagtg     2880

agaatggttt cattaaaatc ttcatggagc aggtccctgg aggaagtctt tctgctctcc     2940

ttcgttccaa atggggtcca ttaaaagaca atgagcaaac aattggcttt tatacaaagc     3000

aaatactgga aggattaaaa tatctccatg acaatcagat agttcaccgg gacataaagg     3060

gtgacaatgt gttgattaat acctacagtg gtgttctcaa gatctctgac ttcggaacat     3120

caaagaggct tgctggcata aacccctgta ctgaaacttt tactggtacc ctccagtata     3180

tggcaccaga aataatagat aaaggaccaa gaggctacgg aaaagcagca gacatctggt     3240

ctctgggctg tacaatcatt gaaatggcca caggaaaacc cccattttat gaactgggag     3300

aaccacaagc agctatgttc aaggtgggaa tgtttaaagt ccaccctgag atcccagagt     3360

ccatgtctgc agaggccaag gcattcatac tgaaatgttt tgaaccagat cctgacaaga     3420

gagcctgtgc taacgacttg cttgttgatg agtttttaaa agtttcaagc aaaaagaaaa     3480

agacacaacc taagctttca gctctttcag ctggatcaaa tgaatatctc aggagtatat     3540

ccttgccggt acctgtgctg gtggaggaca ccagcagcag cagtgagtac ggctcagttt     3600

cacccgacac ggagttgaaa gtggacccct tctctttcaa aacaagagcc aagtcctgcg     3660

gagaaagaga tgtcaaggga attcggacac tctttttggg cattccagat gagaattttg     3720

aagatcacag tgctcctcct tcccctgaag aaaaagattc tggattcttc atgctgagga     3780

aggacagtga gaggcgggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt     3840

tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca     3900

gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct     3960

gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc     4020

tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca     4080

tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga     4140

cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca     4200

tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc     4260

acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc     4320

gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca     4380

tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga     4440

gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg     4500

ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct agatcataat     4560

cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct     4620

gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa     4680

tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca     4740

ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta     4800

atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg     4860

ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg     4920

ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa     4980

aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg     5040

ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt     5100

gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg     5160

ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta     5220

atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta     5280

tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat     5340

aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa     5400

tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag     5460

catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag     5520

aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc     5580

catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt     5640

ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg     5700

aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt     5760

ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc     5820

tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc     5880

tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg     5940

aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag     6000

ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg     6060

ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg     6120

caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac     6180

atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg     6240

acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc     6300

ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg     6360

aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc     6420

aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc     6480

gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc     6540

ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc     6600

caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg     6660

aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt     6720

cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac     6780

ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca     6840

taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg     6900

gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg     6960

cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata     7020

tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct     7080

ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga     7140

ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg     7200

cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc     7260

aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct     7320

agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc     7380

tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt     7440

ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg     7500

cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct     7560

atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag     7620

ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag     7680

tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg     7740

gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg     7800

gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac     7860

cgccatgcat                                                            7870


<210>  28
<211>  1296
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ASK1(g) YFP fusion protein sequence

<400>  28

Met Ser Thr Glu Ala Asp Glu Gly Ile Thr Phe Ser Val Pro Pro Phe 
1               5                   10                  15      


Ala Pro Ser Gly Phe Cys Thr Ile Pro Glu Gly Gly Ile Cys Arg Arg 
            20                  25                  30          


Gly Gly Ala Ala Ala Val Gly Glu Gly Glu Glu His Gln Leu Pro Pro 
        35                  40                  45              


Pro Pro Pro Gly Ser Phe Trp Asn Val Glu Ser Ala Ala Ala Pro Gly 
    50                  55                  60                  


Ile Gly Cys Pro Ala Ala Thr Ser Ser Ser Ser Ala Thr Arg Gly Arg 
65                  70                  75                  80  


Gly Ser Ser Val Gly Gly Gly Ser Arg Arg Thr Thr Val Ala Tyr Val 
                85                  90                  95      


Ile Asn Glu Ala Ser Gln Gly Gln Leu Val Val Ala Glu Ser Glu Ala 
            100                 105                 110         


Leu Gln Ser Leu Arg Glu Ala Cys Glu Thr Val Gly Ala Thr Leu Glu 
        115                 120                 125             


Thr Leu His Phe Gly Lys Leu Asp Phe Gly Glu Thr Thr Val Leu Asp 
    130                 135                 140                 


Arg Phe Tyr Asn Ala Asp Ile Ala Val Val Glu Met Ser Asp Ala Phe 
145                 150                 155                 160 


Arg Gln Pro Ser Leu Phe Tyr His Leu Gly Val Arg Glu Ser Phe Ser 
                165                 170                 175     


Met Ala Asn Asn Ile Ile Leu Tyr Cys Asp Thr Asn Ser Asp Ser Leu 
            180                 185                 190         


Gln Ser Leu Lys Glu Ile Ile Cys Gln Lys Asn Thr Met Cys Thr Gly 
        195                 200                 205             


Asn Tyr Thr Phe Val Pro Tyr Met Ile Thr Pro His Asn Lys Val Tyr 
    210                 215                 220                 


Cys Cys Asp Ser Ser Phe Met Lys Gly Leu Thr Glu Leu Met Gln Pro 
225                 230                 235                 240 


Asn Phe Glu Leu Leu Leu Gly Pro Ile Cys Leu Pro Leu Val Asp Arg 
                245                 250                 255     


Phe Ile Gln Leu Leu Lys Val Ala Gln Ala Ser Ser Ser Gln Tyr Phe 
            260                 265                 270         


Arg Glu Ser Ile Leu Asn Asp Ile Arg Lys Ala Arg Asn Leu Tyr Thr 
        275                 280                 285             


Gly Lys Glu Leu Ala Ala Glu Leu Ala Arg Ile Arg Gln Arg Val Asp 
    290                 295                 300                 


Asn Ile Glu Val Leu Thr Ala Asp Ile Val Ile Asn Leu Leu Leu Ser 
305                 310                 315                 320 


Tyr Arg Asp Ile Gln Asp Tyr Asp Ser Ile Val Lys Leu Val Glu Thr 
                325                 330                 335     


Leu Glu Lys Leu Pro Thr Phe Asp Leu Ala Ser His His His Val Lys 
            340                 345                 350         


Phe His Tyr Ala Phe Ala Leu Asn Arg Arg Asn Leu Pro Gly Asp Arg 
        355                 360                 365             


Ala Lys Ala Leu Asp Ile Met Ile Pro Met Val Gln Ser Glu Gly Gln 
    370                 375                 380                 


Val Ala Ser Asp Met Tyr Cys Leu Val Gly Arg Ile Tyr Lys Asp Met 
385                 390                 395                 400 


Phe Leu Asp Ser Asn Phe Thr Asp Thr Glu Ser Arg Asp His Gly Ala 
                405                 410                 415     


Ser Trp Phe Lys Lys Ala Phe Glu Ser Glu Pro Thr Leu Gln Ser Gly 
            420                 425                 430         


Ile Asn Tyr Ala Val Leu Leu Leu Ala Ala Gly His Gln Phe Glu Ser 
        435                 440                 445             


Ser Phe Glu Leu Arg Lys Val Gly Val Lys Leu Ser Ser Leu Leu Gly 
    450                 455                 460                 


Lys Lys Gly Asn Leu Glu Lys Leu Gln Ser Tyr Trp Glu Val Gly Phe 
465                 470                 475                 480 


Phe Leu Gly Ala Ser Val Leu Ala Asn Asp His Met Arg Val Ile Gln 
                485                 490                 495     


Ala Ser Glu Lys Leu Phe Lys Leu Lys Thr Pro Ala Trp Tyr Leu Lys 
            500                 505                 510         


Ser Ile Val Glu Thr Ile Leu Ile Tyr Lys His Phe Val Lys Leu Thr 
        515                 520                 525             


Thr Glu Gln Pro Val Ala Lys Gln Glu Leu Val Asp Phe Trp Met Asp 
    530                 535                 540                 


Phe Leu Val Glu Ala Thr Lys Thr Asp Val Thr Val Val Arg Phe Pro 
545                 550                 555                 560 


Val Leu Ile Leu Glu Pro Thr Lys Ile Tyr Gln Pro Ser Tyr Leu Ser 
                565                 570                 575     


Ile Asn Asn Glu Val Glu Glu Lys Thr Ile Ser Ile Trp His Val Leu 
            580                 585                 590         


Pro Asp Asp Lys Lys Gly Ile His Glu Trp Asn Phe Ser Ala Ser Ser 
        595                 600                 605             


Val Arg Gly Val Ser Ile Ser Lys Phe Glu Glu Arg Cys Cys Phe Leu 
    610                 615                 620                 


Tyr Val Leu His Asn Ser Asp Asp Phe Gln Ile Tyr Phe Cys Thr Glu 
625                 630                 635                 640 


Leu His Cys Lys Lys Phe Phe Glu Met Val Asn Thr Ile Thr Glu Glu 
                645                 650                 655     


Lys Gly Arg Ser Thr Glu Glu Gly Asp Cys Glu Ser Asp Leu Leu Glu 
            660                 665                 670         


Tyr Asp Tyr Glu Tyr Asp Glu Asn Gly Asp Arg Val Val Leu Gly Lys 
        675                 680                 685             


Gly Thr Tyr Gly Ile Val Tyr Ala Gly Arg Asp Leu Ser Asn Gln Val 
    690                 695                 700                 


Arg Ile Ala Ile Lys Glu Ile Pro Glu Arg Asp Ser Arg Tyr Ser Gln 
705                 710                 715                 720 


Pro Leu His Glu Glu Ile Ala Leu His Lys His Leu Lys His Lys Asn 
                725                 730                 735     


Ile Val Gln Tyr Leu Gly Ser Phe Ser Glu Asn Gly Phe Ile Lys Ile 
            740                 745                 750         


Phe Met Glu Gln Val Pro Gly Gly Ser Leu Ser Ala Leu Leu Arg Ser 
        755                 760                 765             


Lys Trp Gly Pro Leu Lys Asp Asn Glu Gln Thr Ile Gly Phe Tyr Thr 
    770                 775                 780                 


Lys Gln Ile Leu Glu Gly Leu Lys Tyr Leu His Asp Asn Gln Ile Val 
785                 790                 795                 800 


His Arg Asp Ile Lys Gly Asp Asn Val Leu Ile Asn Thr Tyr Ser Gly 
                805                 810                 815     


Val Leu Lys Ile Ser Asp Phe Gly Thr Ser Lys Arg Leu Ala Gly Ile 
            820                 825                 830         


Asn Pro Cys Thr Glu Thr Phe Thr Gly Thr Leu Gln Tyr Met Ala Pro 
        835                 840                 845             


Glu Ile Ile Asp Lys Gly Pro Arg Gly Tyr Gly Lys Ala Ala Asp Ile 
    850                 855                 860                 


Trp Ser Leu Gly Cys Thr Ile Ile Glu Met Ala Thr Gly Lys Pro Pro 
865                 870                 875                 880 


Phe Tyr Glu Leu Gly Glu Pro Gln Ala Ala Met Phe Lys Val Gly Met 
                885                 890                 895     


Phe Lys Val His Pro Glu Ile Pro Glu Ser Met Ser Ala Glu Ala Lys 
            900                 905                 910         


Ala Phe Ile Leu Lys Cys Phe Glu Pro Asp Pro Asp Lys Arg Ala Cys 
        915                 920                 925             


Ala Asn Asp Leu Leu Val Asp Glu Phe Leu Lys Val Ser Ser Lys Lys 
    930                 935                 940                 


Lys Lys Thr Gln Pro Lys Leu Ser Ala Leu Ser Ala Gly Ser Asn Glu 
945                 950                 955                 960 


Tyr Leu Arg Ser Ile Ser Leu Pro Val Pro Val Leu Val Glu Asp Thr 
                965                 970                 975     


Ser Ser Ser Ser Glu Tyr Gly Ser Val Ser Pro Asp Thr Glu Leu Lys 
            980                 985                 990         


Val Asp Pro Phe Ser Phe Lys Thr  Arg Ala Lys Ser Cys  Gly Glu Arg 
        995                 1000                 1005             


Asp Val  Lys Gly Ile Arg Thr  Leu Phe Leu Gly Ile  Pro Asp Glu 
    1010                 1015                 1020             


Asn Phe  Glu Asp His Ser Ala  Pro Pro Ser Pro Glu  Glu Lys Asp 
    1025                 1030                 1035             


Ser Gly  Phe Phe Met Leu Arg  Lys Asp Ser Glu Arg  Arg Asp Pro 
    1040                 1045                 1050             


Pro Val  Ala Thr Met Val Ser  Lys Gly Glu Glu Leu  Phe Thr Gly 
    1055                 1060                 1065             


Val Val  Pro Ile Leu Val Glu  Leu Asp Gly Asp Val  Asn Gly His 
    1070                 1075                 1080             


Lys Phe  Ser Val Ser Gly Glu  Gly Glu Gly Asp Ala  Thr Tyr Gly 
    1085                 1090                 1095             


Lys Leu  Thr Leu Lys Phe Ile  Cys Thr Thr Gly Lys  Leu Pro Val 
    1100                 1105                 1110             


Pro Trp  Pro Thr Leu Val Thr  Thr Phe Gly Tyr Gly  Leu Gln Cys 
    1115                 1120                 1125             


Phe Ala  Arg Tyr Pro Asp His  Met Lys Gln His Asp  Phe Phe Lys 
    1130                 1135                 1140             


Ser Ala  Met Pro Glu Gly Tyr  Val Gln Glu Arg Thr  Ile Phe Phe 
    1145                 1150                 1155             


Lys Asp  Asp Gly Asn Tyr Lys  Thr Arg Ala Glu Val  Lys Phe Glu 
    1160                 1165                 1170             


Gly Asp  Thr Leu Val Asn Arg  Ile Glu Leu Lys Gly  Ile Asp Phe 
    1175                 1180                 1185             


Lys Glu  Asp Gly Asn Ile Leu  Gly His Lys Leu Glu  Tyr Asn Tyr 
    1190                 1195                 1200             


Asn Ser  His Asn Val Tyr Ile  Met Ala Asp Lys Gln  Lys Asn Gly 
    1205                 1210                 1215             


Ile Lys  Val Asn Phe Lys Ile  Arg His Asn Ile Glu  Asp Gly Ser 
    1220                 1225                 1230             


Val Gln  Leu Ala Asp His Tyr  Gln Gln Asn Thr Pro  Ile Gly Asp 
    1235                 1240                 1245             


Gly Pro  Val Leu Leu Pro Asp  Asn His Tyr Leu Ser  Tyr Gln Ser 
    1250                 1255                 1260             


Ala Leu  Ser Lys Asp Pro Asn  Glu Lys Arg Asp His  Met Val Leu 
    1265                 1270                 1275             


Leu Glu  Phe Val Thr Ala Ala  Gly Ile Thr Leu Gly  Met Asp Glu 
    1280                 1285                 1290             


Leu Tyr  Lys 
    1295     


<210>  29
<211>  6919
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct Sequence containing ASK (h) cloned in pEYFP

<400>  29
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggactcag atctcgagct caagcttcga attctgcagt cgacatgctt tcctacagag      660

atatccagga ctatgattct attgtgaagc tggtagagac tttagaaaaa ctgccaacct      720

ttgatttggc ctcccatcac catgtgaagt ttcattatgc atttgcactg aataggagaa      780

atctccctgg tgacagagca aaagctcttg atattatgat tcccatggtg caaagcgaag      840

gacaagttgc ttcagatatg tattgcctag ttggtcgaat ctacaaagat atgtttttgg      900

actctaattt cacggacact gaaagcagag accatggagc ttcttggttc aaaaaggcat      960

ttgaatctga gccaacacta cagtcaggaa ttaattatgc ggtcctcctc ctggcagctg     1020

gacaccagtt tgaatcttcc tttgagctcc ggaaagttgg ggtgaagcta agtagtcttc     1080

ttggtaaaaa gggaaacttg gaaaaactcc agagctactg ggaagttgga ttttttctgg     1140

gggccagcgt cctagccaat gaccacatga gagtcattca agcatctgaa aagcttttta     1200

aactgaagac accagcatgg tacctcaagt ctattgtaga gacaatttta atatataagc     1260

attttgtgaa actgaccaca gaacagcctg tggccaagca agaacttgtg gacttttgga     1320

tggatttcct ggtcgaggcc acaaagacag atgttactgt ggttaggttt ccagtattaa     1380

tattagaacc aaccaaaatc tatcaacctt cttatttgtc tatcaacaat gaagttgagg     1440

aaaagacaat ctctatttgg cacgtgcttc ctgatgacaa gaaaggtata catgagtgga     1500

attttagtgc ctcttctgtc aggggagtga gtatttctaa atttgaagaa agatgctgct     1560

ttctttatgt gcttcacaat tctgatgatt tccaaatcta tttctgtaca gaacttcatt     1620

gtaaaaagtt ttttgagatg gtgaacacca ttaccgaaga gaaggggaga agcacagagg     1680

aaggagactg tgaaagtgac ttgctggagt atgactatga atatgatgaa aatggtgaca     1740

gagtcgtttt aggaaaaggc acttatggga tagtctacgc aggtcgggac ttgagcaacc     1800

aagtcagaat tgctattaag gaaatcccag agagagacag cagatactct cagcccctgc     1860

atgaagaaat agcattgcat aaacacctga agcacaaaaa tattgtccag tatctgggct     1920

ctttcagtga gaatggtttc attaaaatct tcatggagca ggtccctgga ggaagtcttt     1980

ctgctctcct tcgttccaaa tggggtccat taaaagacaa tgagcaaaca attggctttt     2040

atacaaagca aatactggaa ggattaaaat atctccatga caatcagata gttcaccggg     2100

acataaaggg tgacaatgtg ttgattaata cctacagtgg tgttctcaag atctctgact     2160

tcggaacatc aaagaggctt gctggcataa acccctgtac tgaaactttt actggtaccc     2220

tccagtatat ggcaccagaa ataatagata aaggaccaag aggctacgga aaagcagcag     2280

acatctggtc tctgggctgt acaatcattg aaatggccac aggaaaaccc ccattttatg     2340

aactgggaga accacaagca gctatgttca aggtgggaat gtttaaagtc caccctgaga     2400

tcccagagtc catgtctgca gaggccaagg cattcatact gaaatgtttt gaaccagatc     2460

ctgacaagag agcctgtgct aacgacttgc ttgttgatga gtttttaaaa gtttcaagca     2520

aaaagaaaaa gacacaacct aagctttcag ctctttcagc tggatcaaat gaatatctca     2580

ggagtatatc cttgccggta cctgtgctgg tggaggacac cagcagcagc agtgagtacg     2640

gctcagtttc acccgacacg gagttgaaag tggacccctt ctctttcaaa acaagagcca     2700

agtcctgcgg agaaagagat gtcaagggaa ttcggacact ctttttgggc attccagatg     2760

agaattttga agatcacagt gctcctcctt cccctgaaga aaaagattct ggattcttca     2820

tgctgaggaa ggacagtgag aggcgggatc caccggtcgc caccatggtg agcaagggcg     2880

aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc     2940

acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga     3000

agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg     3060

gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca     3120

agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca     3180

actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc     3240

tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact     3300

acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact     3360

tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga     3420

acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt     3480

ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga     3540

ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta     3600

gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca     3660

cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc     3720

agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt     3780

ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg     3840

taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta     3900

accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt     3960

tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca     4020

aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa     4080

gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat     4140

ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag     4200

gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg     4260

ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg     4320

gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat     4380

aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca     4440

gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag     4500

tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc     4560

agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct     4620

aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg     4680

actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa     4740

gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat     4800

gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg     4860

tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg     4920

tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg     4980

ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc     5040

cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg     5100

aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca     5160

tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc     5220

aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg     5280

atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg     5340

cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata     5400

tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg     5460

accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat     5520

gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct     5580

tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca     5640

agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt     5700

gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat     5760

gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc     5820

ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc     5880

gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag     5940

accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc     6000

gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg     6060

ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt     6120

gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg     6180

agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt     6240

aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca     6300

agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac     6360

tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac     6420

atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct     6480

taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg     6540

gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca     6600

gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt     6660

aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta     6720

tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc     6780

gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc     6840

cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa     6900

ccgtattacc gccatgcat                                                  6919


<210>  30
<211>  979
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ASK1(h) YFP fusion protein sequence

<400>  30

Met Leu Ser Tyr Arg Asp Ile Gln Asp Tyr Asp Ser Ile Val Lys Leu 
1               5                   10                  15      


Val Glu Thr Leu Glu Lys Leu Pro Thr Phe Asp Leu Ala Ser His His 
            20                  25                  30          


His Val Lys Phe His Tyr Ala Phe Ala Leu Asn Arg Arg Asn Leu Pro 
        35                  40                  45              


Gly Asp Arg Ala Lys Ala Leu Asp Ile Met Ile Pro Met Val Gln Ser 
    50                  55                  60                  


Glu Gly Gln Val Ala Ser Asp Met Tyr Cys Leu Val Gly Arg Ile Tyr 
65                  70                  75                  80  


Lys Asp Met Phe Leu Asp Ser Asn Phe Thr Asp Thr Glu Ser Arg Asp 
                85                  90                  95      


His Gly Ala Ser Trp Phe Lys Lys Ala Phe Glu Ser Glu Pro Thr Leu 
            100                 105                 110         


Gln Ser Gly Ile Asn Tyr Ala Val Leu Leu Leu Ala Ala Gly His Gln 
        115                 120                 125             


Phe Glu Ser Ser Phe Glu Leu Arg Lys Val Gly Val Lys Leu Ser Ser 
    130                 135                 140                 


Leu Leu Gly Lys Lys Gly Asn Leu Glu Lys Leu Gln Ser Tyr Trp Glu 
145                 150                 155                 160 


Val Gly Phe Phe Leu Gly Ala Ser Val Leu Ala Asn Asp His Met Arg 
                165                 170                 175     


Val Ile Gln Ala Ser Glu Lys Leu Phe Lys Leu Lys Thr Pro Ala Trp 
            180                 185                 190         


Tyr Leu Lys Ser Ile Val Glu Thr Ile Leu Ile Tyr Lys His Phe Val 
        195                 200                 205             


Lys Leu Thr Thr Glu Gln Pro Val Ala Lys Gln Glu Leu Val Asp Phe 
    210                 215                 220                 


Trp Met Asp Phe Leu Val Glu Ala Thr Lys Thr Asp Val Thr Val Val 
225                 230                 235                 240 


Arg Phe Pro Val Leu Ile Leu Glu Pro Thr Lys Ile Tyr Gln Pro Ser 
                245                 250                 255     


Tyr Leu Ser Ile Asn Asn Glu Val Glu Glu Lys Thr Ile Ser Ile Trp 
            260                 265                 270         


His Val Leu Pro Asp Asp Lys Lys Gly Ile His Glu Trp Asn Phe Ser 
        275                 280                 285             


Ala Ser Ser Val Arg Gly Val Ser Ile Ser Lys Phe Glu Glu Arg Cys 
    290                 295                 300                 


Cys Phe Leu Tyr Val Leu His Asn Ser Asp Asp Phe Gln Ile Tyr Phe 
305                 310                 315                 320 


Cys Thr Glu Leu His Cys Lys Lys Phe Phe Glu Met Val Asn Thr Ile 
                325                 330                 335     


Thr Glu Glu Lys Gly Arg Ser Thr Glu Glu Gly Asp Cys Glu Ser Asp 
            340                 345                 350         


Leu Leu Glu Tyr Asp Tyr Glu Tyr Asp Glu Asn Gly Asp Arg Val Val 
        355                 360                 365             


Leu Gly Lys Gly Thr Tyr Gly Ile Val Tyr Ala Gly Arg Asp Leu Ser 
    370                 375                 380                 


Asn Gln Val Arg Ile Ala Ile Lys Glu Ile Pro Glu Arg Asp Ser Arg 
385                 390                 395                 400 


Tyr Ser Gln Pro Leu His Glu Glu Ile Ala Leu His Lys His Leu Lys 
                405                 410                 415     


His Lys Asn Ile Val Gln Tyr Leu Gly Ser Phe Ser Glu Asn Gly Phe 
            420                 425                 430         


Ile Lys Ile Phe Met Glu Gln Val Pro Gly Gly Ser Leu Ser Ala Leu 
        435                 440                 445             


Leu Arg Ser Lys Trp Gly Pro Leu Lys Asp Asn Glu Gln Thr Ile Gly 
    450                 455                 460                 


Phe Tyr Thr Lys Gln Ile Leu Glu Gly Leu Lys Tyr Leu His Asp Asn 
465                 470                 475                 480 


Gln Ile Val His Arg Asp Ile Lys Gly Asp Asn Val Leu Ile Asn Thr 
                485                 490                 495     


Tyr Ser Gly Val Leu Lys Ile Ser Asp Phe Gly Thr Ser Lys Arg Leu 
            500                 505                 510         


Ala Gly Ile Asn Pro Cys Thr Glu Thr Phe Thr Gly Thr Leu Gln Tyr 
        515                 520                 525             


Met Ala Pro Glu Ile Ile Asp Lys Gly Pro Arg Gly Tyr Gly Lys Ala 
    530                 535                 540                 


Ala Asp Ile Trp Ser Leu Gly Cys Thr Ile Ile Glu Met Ala Thr Gly 
545                 550                 555                 560 


Lys Pro Pro Phe Tyr Glu Leu Gly Glu Pro Gln Ala Ala Met Phe Lys 
                565                 570                 575     


Val Gly Met Phe Lys Val His Pro Glu Ile Pro Glu Ser Met Ser Ala 
            580                 585                 590         


Glu Ala Lys Ala Phe Ile Leu Lys Cys Phe Glu Pro Asp Pro Asp Lys 
        595                 600                 605             


Arg Ala Cys Ala Asn Asp Leu Leu Val Asp Glu Phe Leu Lys Val Ser 
    610                 615                 620                 


Ser Lys Lys Lys Lys Thr Gln Pro Lys Leu Ser Ala Leu Ser Ala Gly 
625                 630                 635                 640 


Ser Asn Glu Tyr Leu Arg Ser Ile Ser Leu Pro Val Pro Val Leu Val 
                645                 650                 655     


Glu Asp Thr Ser Ser Ser Ser Glu Tyr Gly Ser Val Ser Pro Asp Thr 
            660                 665                 670         


Glu Leu Lys Val Asp Pro Phe Ser Phe Lys Thr Arg Ala Lys Ser Cys 
        675                 680                 685             


Gly Glu Arg Asp Val Lys Gly Ile Arg Thr Leu Phe Leu Gly Ile Pro 
    690                 695                 700                 


Asp Glu Asn Phe Glu Asp His Ser Ala Pro Pro Ser Pro Glu Glu Lys 
705                 710                 715                 720 


Asp Ser Gly Phe Phe Met Leu Arg Lys Asp Ser Glu Arg Arg Asp Pro 
                725                 730                 735     


Pro Val Ala Thr Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val 
            740                 745                 750         


Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe 
        755                 760                 765             


Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr 
    770                 775                 780                 


Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr 
785                 790                 795                 800 


Leu Val Thr Thr Phe Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro 
                805                 810                 815     


Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly 
            820                 825                 830         


Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys 
        835                 840                 845             


Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile 
    850                 855                 860                 


Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His 
865                 870                 875                 880 


Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp 
                885                 890                 895     


Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile 
            900                 905                 910         


Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro 
        915                 920                 925             


Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr 
    930                 935                 940                 


Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val 
945                 950                 955                 960 


Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu 
                965                 970                 975     


Leu Tyr Lys 
            


<210>  31
<211>  7429
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct Sequence containing ASK (i) cloned in pEYFP

<400>  31
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggactcag atctcgagct caagcttcga attctgcagt cgacatgagc acggaggcgg      660

acgagggcat cactttctct gtgccaccct tcgccccctc gggcttctgc accatccccg      720

agggcggcat ctgcaggagg ggaggagcgg cggcggtggg cgagggcgag gagcaccagc      780

tgccaccgcc gccgccgggc agtttctgga acgtggagag cgccgctgcc cctggcatcg      840

gttgtccggc ggccacctcc tcgagcagtg ccacccgagg ccggggcagc tctgttggcg      900

ggggcagccg acggaccacg gtggcatatg tgatcaacga agcgagccaa gggcaactgg      960

tggtggccga gagcgaggcc ctgcagagct tgcgggaggc gtgcgagaca gtgggcgcca     1020

ccctggaaac cctgcatttt gggaaactcg actttggaga aaccaccgtg ctggaccgct     1080

tttacaatgc agatattgcg gtggtggaga tgagcgatgc cttccggcag ccgtccttgt     1140

tttaccacct tggggtgaga gaaagtttca gcatggccaa caacatcatc ctctactgtg     1200

atactaactc ggactctctg cagtcactga aggaaatcat ttgccagaag aatactatgt     1260

gcactgggaa ctacaccttt gttccttaca tgataactcc acataacaaa gtctactgct     1320

gtgacagcag cttcatgaag gggttgacag agctcatgca accgaacttc gagctgcttc     1380

ttggacccat ctgcttacct cttgtggatc gttttattca acttttgaag gtggcacaag     1440

caagttctag ccagtacttc cgggaatcta tactcaatga catcaggaaa gctcgtaatt     1500

tatacactgg taaagaattg gcagctgagt tggcaagaat tcggcagcga gtagataata     1560

tcgaagtctt gacagcagat attgtcataa atctgttact ttcctacaga gatatccagg     1620

actatgattc tattgtgaag ctggtagaga ctttagaaaa actgccaacc tttgatttgg     1680

cctcccatca ccatgtgaag tttcattatg catttgcact gaataggaga aatctccctg     1740

gtgacagagc aaaagctctt gatattatga ttcccatggt gcaaagcgaa ggacaagttg     1800

cttcagatat gtattgccta gttggtcgaa tctacaaaga tatgtttttg gactctaatt     1860

tcacggacac tgaaagcaga gaccatggag cttcttggtt caaaaaggca tttgaatctg     1920

agccaacact acagtcagga attaattatg cggtcctcct cctggcagct ggacaccagt     1980

ttgaatcttc ctttgagctc cggaaagttg gggtgaagct aagtagtctt cttggtaaaa     2040

agggaaactt ggaaaaactc cagagctact gggaagttgg attttttctg ggggccagcg     2100

tcctagccaa tgaccacatg agagtcattc aagcatctga aaagcttttt aaactgaaga     2160

caccagcatg gtacctcaag tctattgtag agacaatttt aatatataag cattttgtga     2220

aactgaccac agaacagcct gtggccaagc aagaacttgt ggacttttgg atggatttcc     2280

tggtcgaggc cacaaagaca gatgttactg tggttaggtt tccagtatta atattagaac     2340

caaccaaaat ctatcaacct tcttatttgt ctatcaacaa tgaagttgag gaaaagacaa     2400

tctctatttg gcacgtgctt cctgatgaca agaaaggtat acatgagtgg aattttagtg     2460

cctcttctgt caggggagtg agtatttcta aatttgaaga aagatgctgc tttctttatg     2520

tgcttcacaa ttctgatgat ttccaaatct atttctgtac agaacttcat tgtaaaaagt     2580

tttttgagat ggtgaacacc attaccgaag agaaggggag aagcacagag gaaggagact     2640

gtgaaagtga cttgctggag tatgactatg aatatgatga aaatggtgac agagtcgttt     2700

taggaaaagg cacttatggg atagtctacg caggtcggga cttgagcaac caagtcagaa     2760

ttgctattaa ggaaatccca gagagagaca gcagatactc tcagcccctg catgaagaaa     2820

tagcattgca taaacacctg aagcacaaaa atattgtcca gtatctgggc tctttcagtg     2880

agaatggttt cattaaaatc ttcatggagc aggtccctgg aggaagtctt tctgctctcc     2940

ttcgttccaa atggggtcca ttaaaagaca atgagcaaac aattggcttt tatacaaagc     3000

aaatactgga aggattaaaa tatctccatg acaatcagat agttcaccgg gacataaagg     3060

gtgacaatgt gttgattaat acctacagtg gtgttctcaa gatctctgac ttcggaacat     3120

caaagaggct tgctggcata aacccctgta ctgaaacttt tactggtacc ctccagtata     3180

tggcaccaga aataatagat aaaggaccaa gaggctacgg aaaagcagca gacatctggt     3240

ctctgggctg tacaatcatt gaaatggcca caggaaaacc cccattttat gaactgggag     3300

aaccacaagc agctatgttc aaggtgggaa tgtttaaagt ccaccctgag atccgggatc     3360

caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc     3420

tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg     3480

gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg     3540

tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc gcccgctacc     3600

ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg     3660

agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg     3720

agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca     3780

acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg     3840

acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca     3900

gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc     3960

tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc     4020

gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg     4080

agctgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca catttgtaga     4140

ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa     4200

tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag     4260

catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa     4320

actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt     4380

taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt     4440

ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc     4500

cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg     4560

gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac     4620

taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg     4680

tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag     4740

cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt     4800

caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac     4860

attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa     4920

aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg     4980

aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc     5040

aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct     5100

caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc     5160

cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga     5220

ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg     5280

cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg     5340

gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac     5400

aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg     5460

ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc     5520

ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg     5580

aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc     5640

accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc     5700

ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta     5760

ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg     5820

cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg     5880

tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat     5940

tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc     6000

gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta     6060

tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag     6120

cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt     6180

cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg     6240

ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag     6300

gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa     6360

aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca     6420

gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc     6480

ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt     6540

cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa     6600

acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa     6660

aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg     6720

atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc     6780

gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac     6840

tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca     6900

ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt     6960

ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc     7020

ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg     7080

aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc     7140

cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac     7200

gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct     7260

ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc     7320

cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt     7380

tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat                 7429


<210>  32
<211>  1149
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ASK1(I) YFP fusion protein sequence

<400>  32

Met Ser Thr Glu Ala Asp Glu Gly Ile Thr Phe Ser Val Pro Pro Phe 
1               5                   10                  15      


Ala Pro Ser Gly Phe Cys Thr Ile Pro Glu Gly Gly Ile Cys Arg Arg 
            20                  25                  30          


Gly Gly Ala Ala Ala Val Gly Glu Gly Glu Glu His Gln Leu Pro Pro 
        35                  40                  45              


Pro Pro Pro Gly Ser Phe Trp Asn Val Glu Ser Ala Ala Ala Pro Gly 
    50                  55                  60                  


Ile Gly Cys Pro Ala Ala Thr Ser Ser Ser Ser Ala Thr Arg Gly Arg 
65                  70                  75                  80  


Gly Ser Ser Val Gly Gly Gly Ser Arg Arg Thr Thr Val Ala Tyr Val 
                85                  90                  95      


Ile Asn Glu Ala Ser Gln Gly Gln Leu Val Val Ala Glu Ser Glu Ala 
            100                 105                 110         


Leu Gln Ser Leu Arg Glu Ala Cys Glu Thr Val Gly Ala Thr Leu Glu 
        115                 120                 125             


Thr Leu His Phe Gly Lys Leu Asp Phe Gly Glu Thr Thr Val Leu Asp 
    130                 135                 140                 


Arg Phe Tyr Asn Ala Asp Ile Ala Val Val Glu Met Ser Asp Ala Phe 
145                 150                 155                 160 


Arg Gln Pro Ser Leu Phe Tyr His Leu Gly Val Arg Glu Ser Phe Ser 
                165                 170                 175     


Met Ala Asn Asn Ile Ile Leu Tyr Cys Asp Thr Asn Ser Asp Ser Leu 
            180                 185                 190         


Gln Ser Leu Lys Glu Ile Ile Cys Gln Lys Asn Thr Met Cys Thr Gly 
        195                 200                 205             


Asn Tyr Thr Phe Val Pro Tyr Met Ile Thr Pro His Asn Lys Val Tyr 
    210                 215                 220                 


Cys Cys Asp Ser Ser Phe Met Lys Gly Leu Thr Glu Leu Met Gln Pro 
225                 230                 235                 240 


Asn Phe Glu Leu Leu Leu Gly Pro Ile Cys Leu Pro Leu Val Asp Arg 
                245                 250                 255     


Phe Ile Gln Leu Leu Lys Val Ala Gln Ala Ser Ser Ser Gln Tyr Phe 
            260                 265                 270         


Arg Glu Ser Ile Leu Asn Asp Ile Arg Lys Ala Arg Asn Leu Tyr Thr 
        275                 280                 285             


Gly Lys Glu Leu Ala Ala Glu Leu Ala Arg Ile Arg Gln Arg Val Asp 
    290                 295                 300                 


Asn Ile Glu Val Leu Thr Ala Asp Ile Val Ile Asn Leu Leu Leu Ser 
305                 310                 315                 320 


Tyr Arg Asp Ile Gln Asp Tyr Asp Ser Ile Val Lys Leu Val Glu Thr 
                325                 330                 335     


Leu Glu Lys Leu Pro Thr Phe Asp Leu Ala Ser His His His Val Lys 
            340                 345                 350         


Phe His Tyr Ala Phe Ala Leu Asn Arg Arg Asn Leu Pro Gly Asp Arg 
        355                 360                 365             


Ala Lys Ala Leu Asp Ile Met Ile Pro Met Val Gln Ser Glu Gly Gln 
    370                 375                 380                 


Val Ala Ser Asp Met Tyr Cys Leu Val Gly Arg Ile Tyr Lys Asp Met 
385                 390                 395                 400 


Phe Leu Asp Ser Asn Phe Thr Asp Thr Glu Ser Arg Asp His Gly Ala 
                405                 410                 415     


Ser Trp Phe Lys Lys Ala Phe Glu Ser Glu Pro Thr Leu Gln Ser Gly 
            420                 425                 430         


Ile Asn Tyr Ala Val Leu Leu Leu Ala Ala Gly His Gln Phe Glu Ser 
        435                 440                 445             


Ser Phe Glu Leu Arg Lys Val Gly Val Lys Leu Ser Ser Leu Leu Gly 
    450                 455                 460                 


Lys Lys Gly Asn Leu Glu Lys Leu Gln Ser Tyr Trp Glu Val Gly Phe 
465                 470                 475                 480 


Phe Leu Gly Ala Ser Val Leu Ala Asn Asp His Met Arg Val Ile Gln 
                485                 490                 495     


Ala Ser Glu Lys Leu Phe Lys Leu Lys Thr Pro Ala Trp Tyr Leu Lys 
            500                 505                 510         


Ser Ile Val Glu Thr Ile Leu Ile Tyr Lys His Phe Val Lys Leu Thr 
        515                 520                 525             


Thr Glu Gln Pro Val Ala Lys Gln Glu Leu Val Asp Phe Trp Met Asp 
    530                 535                 540                 


Phe Leu Val Glu Ala Thr Lys Thr Asp Val Thr Val Val Arg Phe Pro 
545                 550                 555                 560 


Val Leu Ile Leu Glu Pro Thr Lys Ile Tyr Gln Pro Ser Tyr Leu Ser 
                565                 570                 575     


Ile Asn Asn Glu Val Glu Glu Lys Thr Ile Ser Ile Trp His Val Leu 
            580                 585                 590         


Pro Asp Asp Lys Lys Gly Ile His Glu Trp Asn Phe Ser Ala Ser Ser 
        595                 600                 605             


Val Arg Gly Val Ser Ile Ser Lys Phe Glu Glu Arg Cys Cys Phe Leu 
    610                 615                 620                 


Tyr Val Leu His Asn Ser Asp Asp Phe Gln Ile Tyr Phe Cys Thr Glu 
625                 630                 635                 640 


Leu His Cys Lys Lys Phe Phe Glu Met Val Asn Thr Ile Thr Glu Glu 
                645                 650                 655     


Lys Gly Arg Ser Thr Glu Glu Gly Asp Cys Glu Ser Asp Leu Leu Glu 
            660                 665                 670         


Tyr Asp Tyr Glu Tyr Asp Glu Asn Gly Asp Arg Val Val Leu Gly Lys 
        675                 680                 685             


Gly Thr Tyr Gly Ile Val Tyr Ala Gly Arg Asp Leu Ser Asn Gln Val 
    690                 695                 700                 


Arg Ile Ala Ile Lys Glu Ile Pro Glu Arg Asp Ser Arg Tyr Ser Gln 
705                 710                 715                 720 


Pro Leu His Glu Glu Ile Ala Leu His Lys His Leu Lys His Lys Asn 
                725                 730                 735     


Ile Val Gln Tyr Leu Gly Ser Phe Ser Glu Asn Gly Phe Ile Lys Ile 
            740                 745                 750         


Phe Met Glu Gln Val Pro Gly Gly Ser Leu Ser Ala Leu Leu Arg Ser 
        755                 760                 765             


Lys Trp Gly Pro Leu Lys Asp Asn Glu Gln Thr Ile Gly Phe Tyr Thr 
    770                 775                 780                 


Lys Gln Ile Leu Glu Gly Leu Lys Tyr Leu His Asp Asn Gln Ile Val 
785                 790                 795                 800 


His Arg Asp Ile Lys Gly Asp Asn Val Leu Ile Asn Thr Tyr Ser Gly 
                805                 810                 815     


Val Leu Lys Ile Ser Asp Phe Gly Thr Ser Lys Arg Leu Ala Gly Ile 
            820                 825                 830         


Asn Pro Cys Thr Glu Thr Phe Thr Gly Thr Leu Gln Tyr Met Ala Pro 
        835                 840                 845             


Glu Ile Ile Asp Lys Gly Pro Arg Gly Tyr Gly Lys Ala Ala Asp Ile 
    850                 855                 860                 


Trp Ser Leu Gly Cys Thr Ile Ile Glu Met Ala Thr Gly Lys Pro Pro 
865                 870                 875                 880 


Phe Tyr Glu Leu Gly Glu Pro Gln Ala Ala Met Phe Lys Val Gly Met 
                885                 890                 895     


Phe Lys Val His Pro Glu Ile Arg Asp Pro Pro Val Ala Thr Met Val 
            900                 905                 910         


Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu 
        915                 920                 925             


Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly 
    930                 935                 940                 


Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr 
945                 950                 955                 960 


Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe Gly 
                965                 970                 975     


Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln His 
            980                 985                 990         


Asp Phe Phe Lys Ser Ala Met Pro  Glu Gly Tyr Val Gln  Glu Arg Thr 
        995                 1000                 1005             


Ile Phe  Phe Lys Asp Asp Gly  Asn Tyr Lys Thr Arg  Ala Glu Val 
    1010                 1015                 1020             


Lys Phe  Glu Gly Asp Thr Leu  Val Asn Arg Ile Glu  Leu Lys Gly 
    1025                 1030                 1035             


Ile Asp  Phe Lys Glu Asp Gly  Asn Ile Leu Gly His  Lys Leu Glu 
    1040                 1045                 1050             


Tyr Asn  Tyr Asn Ser His Asn  Val Tyr Ile Met Ala  Asp Lys Gln 
    1055                 1060                 1065             


Lys Asn  Gly Ile Lys Val Asn  Phe Lys Ile Arg His  Asn Ile Glu 
    1070                 1075                 1080             


Asp Gly  Ser Val Gln Leu Ala  Asp His Tyr Gln Gln  Asn Thr Pro 
    1085                 1090                 1095             


Ile Gly  Asp Gly Pro Val Leu  Leu Pro Asp Asn His  Tyr Leu Ser 
    1100                 1105                 1110             


Tyr Gln  Ser Ala Leu Ser Lys  Asp Pro Asn Glu Lys  Arg Asp His 
    1115                 1120                 1125             


Met Val  Leu Leu Glu Phe Val  Thr Ala Ala Gly Ile  Thr Leu Gly 
    1130                 1135                 1140             


Met Asp  Glu Leu Tyr Lys 
    1145                 


<210>  33
<211>  6478
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  construct Sequence containing ASK (J) cloned in pEYFP

<400>  33
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggactcag atctcgagct caagcttcga attctgcagt cgacatgctt tcctacagag      660

atatccagga ctatgattct attgtgaagc tggtagagac tttagaaaaa ctgccaacct      720

ttgatttggc ctcccatcac catgtgaagt ttcattatgc atttgcactg aataggagaa      780

atctccctgg tgacagagca aaagctcttg atattatgat tcccatggtg caaagcgaag      840

gacaagttgc ttcagatatg tattgcctag ttggtcgaat ctacaaagat atgtttttgg      900

actctaattt cacggacact gaaagcagag accatggagc ttcttggttc aaaaaggcat      960

ttgaatctga gccaacacta cagtcaggaa ttaattatgc ggtcctcctc ctggcagctg     1020

gacaccagtt tgaatcttcc tttgagctcc ggaaagttgg ggtgaagcta agtagtcttc     1080

ttggtaaaaa gggaaacttg gaaaaactcc agagctactg ggaagttgga ttttttctgg     1140

gggccagcgt cctagccaat gaccacatga gagtcattca agcatctgaa aagcttttta     1200

aactgaagac accagcatgg tacctcaagt ctattgtaga gacaatttta atatataagc     1260

attttgtgaa actgaccaca gaacagcctg tggccaagca agaacttgtg gacttttgga     1320

tggatttcct ggtcgaggcc acaaagacag atgttactgt ggttaggttt ccagtattaa     1380

tattagaacc aaccaaaatc tatcaacctt cttatttgtc tatcaacaat gaagttgagg     1440

aaaagacaat ctctatttgg cacgtgcttc ctgatgacaa gaaaggtata catgagtgga     1500

attttagtgc ctcttctgtc aggggagtga gtatttctaa atttgaagaa agatgctgct     1560

ttctttatgt gcttcacaat tctgatgatt tccaaatcta tttctgtaca gaacttcatt     1620

gtaaaaagtt ttttgagatg gtgaacacca ttaccgaaga gaaggggaga agcacagagg     1680

aaggagactg tgaaagtgac ttgctggagt atgactatga atatgatgaa aatggtgaca     1740

gagtcgtttt aggaaaaggc acttatggga tagtctacgc aggtcgggac ttgagcaacc     1800

aagtcagaat tgctattaag gaaatcccag agagagacag cagatactct cagcccctgc     1860

atgaagaaat agcattgcat aaacacctga agcacaaaaa tattgtccag tatctgggct     1920

ctttcagtga gaatggtttc attaaaatct tcatggagca ggtccctgga ggaagtcttt     1980

ctgctctcct tcgttccaaa tggggtccat taaaagacaa tgagcaaaca attggctttt     2040

atacaaagca aatactggaa ggattaaaat atctccatga caatcagata gttcaccggg     2100

acataaaggg tgacaatgtg ttgattaata cctacagtgg tgttctcaag atctctgact     2160

tcggaacatc aaagaggctt gctggcataa acccctgtac tgaaactttt actggtaccc     2220

tccagtatat ggcaccagaa ataatagata aaggaccaag aggctacgga aaagcagcag     2280

acatctggtc tctgggctgt acaatcattg aaatggccac aggaaaaccc ccattttatg     2340

aactgggaga accacaagca gctatgttca aggtgggaat gtttaaagtc caccctgaga     2400

tccgggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg     2460

tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg     2520

agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca     2580

agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg cagtgcttcg     2640

cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct     2700

acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg     2760

tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg     2820

aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata     2880

tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg     2940

aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc     3000

ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca     3060

acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg     3120

gcatggacga gctgtacaag taaagcggcc gcgactctag atcataatca gccataccac     3180

atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca     3240

taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata     3300

aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg     3360

tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa     3420

aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca     3480

aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga     3540

acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc     3600

agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc     3660

gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc     3720

cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg     3780

caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac     3840

agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt     3900

tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat     3960

aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt     4020

agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa     4080

ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag     4140

catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct     4200

aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc     4260

agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg     4320

aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg     4380

aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg     4440

actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg     4500

ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg     4560

aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg     4620

ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc     4680

tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc     4740

tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc     4800

gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc     4860

aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg     4920

atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct     4980

tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt     5040

tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc     5100

tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt     5160

tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc     5220

acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg     5280

ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc     5340

tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac     5400

ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt     5460

tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc     5520

ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc     5580

agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat     5640

tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct     5700

catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa     5760

gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa     5820

aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc     5880

gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta     5940

gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct     6000

gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg     6060

atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag     6120

cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc     6180

cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg     6240

agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt     6300

tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg     6360

gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca     6420

catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat       6478


<210>  34
<211>  832
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ASK1(j) YFP fusion protein sequence

<400>  34

Met Leu Ser Tyr Arg Asp Ile Gln Asp Tyr Asp Ser Ile Val Lys Leu 
1               5                   10                  15      


Val Glu Thr Leu Glu Lys Leu Pro Thr Phe Asp Leu Ala Ser His His 
            20                  25                  30          


His Val Lys Phe His Tyr Ala Phe Ala Leu Asn Arg Arg Asn Leu Pro 
        35                  40                  45              


Gly Asp Arg Ala Lys Ala Leu Asp Ile Met Ile Pro Met Val Gln Ser 
    50                  55                  60                  


Glu Gly Gln Val Ala Ser Asp Met Tyr Cys Leu Val Gly Arg Ile Tyr 
65                  70                  75                  80  


Lys Asp Met Phe Leu Asp Ser Asn Phe Thr Asp Thr Glu Ser Arg Asp 
                85                  90                  95      


His Gly Ala Ser Trp Phe Lys Lys Ala Phe Glu Ser Glu Pro Thr Leu 
            100                 105                 110         


Gln Ser Gly Ile Asn Tyr Ala Val Leu Leu Leu Ala Ala Gly His Gln 
        115                 120                 125             


Phe Glu Ser Ser Phe Glu Leu Arg Lys Val Gly Val Lys Leu Ser Ser 
    130                 135                 140                 


Leu Leu Gly Lys Lys Gly Asn Leu Glu Lys Leu Gln Ser Tyr Trp Glu 
145                 150                 155                 160 


Val Gly Phe Phe Leu Gly Ala Ser Val Leu Ala Asn Asp His Met Arg 
                165                 170                 175     


Val Ile Gln Ala Ser Glu Lys Leu Phe Lys Leu Lys Thr Pro Ala Trp 
            180                 185                 190         


Tyr Leu Lys Ser Ile Val Glu Thr Ile Leu Ile Tyr Lys His Phe Val 
        195                 200                 205             


Lys Leu Thr Thr Glu Gln Pro Val Ala Lys Gln Glu Leu Val Asp Phe 
    210                 215                 220                 


Trp Met Asp Phe Leu Val Glu Ala Thr Lys Thr Asp Val Thr Val Val 
225                 230                 235                 240 


Arg Phe Pro Val Leu Ile Leu Glu Pro Thr Lys Ile Tyr Gln Pro Ser 
                245                 250                 255     


Tyr Leu Ser Ile Asn Asn Glu Val Glu Glu Lys Thr Ile Ser Ile Trp 
            260                 265                 270         


His Val Leu Pro Asp Asp Lys Lys Gly Ile His Glu Trp Asn Phe Ser 
        275                 280                 285             


Ala Ser Ser Val Arg Gly Val Ser Ile Ser Lys Phe Glu Glu Arg Cys 
    290                 295                 300                 


Cys Phe Leu Tyr Val Leu His Asn Ser Asp Asp Phe Gln Ile Tyr Phe 
305                 310                 315                 320 


Cys Thr Glu Leu His Cys Lys Lys Phe Phe Glu Met Val Asn Thr Ile 
                325                 330                 335     


Thr Glu Glu Lys Gly Arg Ser Thr Glu Glu Gly Asp Cys Glu Ser Asp 
            340                 345                 350         


Leu Leu Glu Tyr Asp Tyr Glu Tyr Asp Glu Asn Gly Asp Arg Val Val 
        355                 360                 365             


Leu Gly Lys Gly Thr Tyr Gly Ile Val Tyr Ala Gly Arg Asp Leu Ser 
    370                 375                 380                 


Asn Gln Val Arg Ile Ala Ile Lys Glu Ile Pro Glu Arg Asp Ser Arg 
385                 390                 395                 400 


Tyr Ser Gln Pro Leu His Glu Glu Ile Ala Leu His Lys His Leu Lys 
                405                 410                 415     


His Lys Asn Ile Val Gln Tyr Leu Gly Ser Phe Ser Glu Asn Gly Phe 
            420                 425                 430         


Ile Lys Ile Phe Met Glu Gln Val Pro Gly Gly Ser Leu Ser Ala Leu 
        435                 440                 445             


Leu Arg Ser Lys Trp Gly Pro Leu Lys Asp Asn Glu Gln Thr Ile Gly 
    450                 455                 460                 


Phe Tyr Thr Lys Gln Ile Leu Glu Gly Leu Lys Tyr Leu His Asp Asn 
465                 470                 475                 480 


Gln Ile Val His Arg Asp Ile Lys Gly Asp Asn Val Leu Ile Asn Thr 
                485                 490                 495     


Tyr Ser Gly Val Leu Lys Ile Ser Asp Phe Gly Thr Ser Lys Arg Leu 
            500                 505                 510         


Ala Gly Ile Asn Pro Cys Thr Glu Thr Phe Thr Gly Thr Leu Gln Tyr 
        515                 520                 525             


Met Ala Pro Glu Ile Ile Asp Lys Gly Pro Arg Gly Tyr Gly Lys Ala 
    530                 535                 540                 


Ala Asp Ile Trp Ser Leu Gly Cys Thr Ile Ile Glu Met Ala Thr Gly 
545                 550                 555                 560 


Lys Pro Pro Phe Tyr Glu Leu Gly Glu Pro Gln Ala Ala Met Phe Lys 
                565                 570                 575     


Val Gly Met Phe Lys Val His Pro Glu Ile Arg Asp Pro Pro Val Ala 
            580                 585                 590         


Thr Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile 
        595                 600                 605             


Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser 
    610                 615                 620                 


Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe 
625                 630                 635                 640 


Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr 
                645                 650                 655     


Thr Phe Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His Met 
            660                 665                 670         


Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln 
        675                 680                 685             


Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala 
    690                 695                 700                 


Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys 
705                 710                 715                 720 


Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu 
                725                 730                 735     


Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys 
            740                 745                 750         


Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly 
        755                 760                 765             


Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp 
    770                 775                 780                 


Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala 
785                 790                 795                 800 


Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu 
                805                 810                 815     


Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
            820                 825                 830         


<210>  35
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer N1: HIV-1 nef gene clooned in VP16pcDNA+pACT

<400>  35
catggatccg tggagcactt acaagcagca                                        30


<210>  36
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer N1: HIV-1 nef gene cloned in VP16pcDNA+pACT

<400>  36
catggatcct cagcagtctt tgtaatactc c                                      31


<210>  37
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer 1: ASK1(a) gene cloned in pBIND

<400>  37
agcggatccg tacggaggcg gacgagggca tca                                    33


<210>  38
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer 1: ASK1(a) gene cloned in pBIND

<400>  38
agcgtctaga caaatcaaag gttggcag                                          28


<210>  39
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer 2: ASK1(b) gene cloned in pBIND

<400>  39
agcggatccg ttcctacaga gatatccagg ac                                     32


<210>  40
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer 2: ASK1(b) gene cloned in pBIND

<400>  40
agcgtctaga caagtcactt tcacagtctc                                        30


<210>  41
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer 3: ASK1(c) gene cloned in pBIND

<400>  41
agcggatccg ttcttctgtc aggggagtga                                        30


<210>  42
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer 3: ASK1(c) gene cloned in pBIND

<400>  42
agcgtctaga tgggatctca gggtggac                                          28


<210>  43
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer 4: ASK1(d) gene cloned in pBIND

<400>  43
agcggatccg tgcagcagac atctggtctc                                        30


<210>  44
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer 3420: ASK1(d) gene cloned in pBIND

<400>  44
agcgtctaga actctcagat gcaaggctg                                         29


<210>  45
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer 4: ASK1(e) gene cloned in pBIND

<400>  45
agcggatccg tgacagtatc attcggaagg cgg                                    33


<210>  46
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer 4: ASK1(e) gene cloned in pBIND

<400>  46
agcgtctaga gtcaatgata gccttccaca g                                      31


<210>  47
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer 5: ASK1(f) gene cloned in pBIND

<400>  47
agcggatccg tacggaggcg gacgagggca tca                                    33


<210>  48
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer 5: ASK1(f) gene cloned in pBIND

<400>  48
agcgtctaga tcgcctctca ctgtccttcc                                        30


<210>  49
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer 1: ASK1(g) gene cloned in pBIND

<400>  49
agcggatccg tacggaggcg gacgagggca tca                                    33


<210>  50
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer 3420: ASK1(g) gene cloned in pBIND

<400>  50
agcgtctaga actctcagat gcaaggctg                                         29


<210>  51
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer 2: ASK1(h) gene cloned in pBIND

<400>  51
agcggatccg ttcctacaga gatatccagg ac                                     32


<210>  52
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer 3420: ASK1(h) gene cloned in pBIND

<400>  52
agcgtctaga actctcagat gcaaggctg                                         29


<210>  53
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer 1: ASK1(i) gene cloned in pBIND

<400>  53
agcggatccg tacggaggcg gacgagggca tca                                    33


<210>  54
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer 3: ASK1(i) gene cloned in pBIND

<400>  54
agcgtctaga tgggatctca gggtggac                                          28


<210>  55
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer 2: ASK1(J) gene cloned in pBIND

<400>  55
agcggatccg ttcctacaga gatatccagg ac                                     32


<210>  56
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer 3: ASK1(J) gene cloned in pBIND

<400>  56
agcgtctaga tgggatctca gggtggac                                          28


<210>  57
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer 1: HIV-1 nef gene cloned in pEYFP-N1

<400>  57
agcaagctta tggggggcaa gtggtcaaaa                                        30


<210>  58
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer 1: HIV-1 nef gene cloned in pEYFP-N1

<400>  58
agcgtcgacg cgcagtcttt gtaaactccg                                        30


<210>  59
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer 2: ASK1(g) cloned in pEYFP-N1

<400>  59
gtcgaatgag cacggaggcg acgagggcat                                        30


<210>  60
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer 2: ASK1(g) cloned in pEYFP-N1

<400>  60
ggatcccgcc tctcactgtc cttcctcagc atg                                    33


<210>  61
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer 3: ASK1(h) cloned in pEYFP-N1

<400>  61
cgagcgtcga catgtcctac agagatatcc aggactatga                             40


<210>  62
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer 3: ASK1(h) cloned in pEYFP-N1

<400>  62
ggatcccgcc tctcactgtc cttcctcagc atg                                    33


<210>  63
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer 4: ASK1(i) cloned in pEYFP-N1

<400>  63
gtcgaatgag cacggaggcg gacgagggca t                                      31


<210>  64
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer 4: ASK1(i) cloned in pEYFP-N1

<400>  64
agcggatcct ctgggatctc agggtggact t                                      31


<210>  65
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer 5: ASK1(J) cloned in pEYFP-N1

<400>  65
cgagcgtcga catgtcctac agagatatcc aggactatga                             40


<210>  66
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer 5: ASK1(J) cloned in pEYFP-N1

<400>  66
agcggatcct ctgggatctc agggtggact t                                      31


<210>  67
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide 1: Inhibitor reversing Nef-ASK1 effect

<400>  67

Thr Pro Gly Pro Gly Val Arg Tyr Pro 
1               5                   


<210>  68
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide 2: Inhibitor reversing Nef-ASK1 effect

<400>  68

Gly Glu Asn Asn Cys Leu Leu His 
1               5               


<210>  69
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide 3: Inhibitor reversing Nef-ASK1 effect

<400>  69

Asp Glu Val Gly Glu Ala Asn Asn 
1               5               


<210>  70
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide 4: Inhibitor reversing Nef-ASK1 effect

<400>  70

Leu His Gly Met Glu Asp Pro Glu 
1               5               


<210>  71
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide 5: Inhibitor reversing Nef-ASK1 effect

<400>  71

Pro Val Arg Pro Gln Val Pro Leu Arg Pro 
1               5                   10  


