                         SEQUENCE LISTING

<110>  Institut Pasteur
       Centre National de la Recherche Scientifique
       MAZEL Didier
       BIKARD David
       GRIEB Maj Svea
 
<120>  De novo integron recombination sites and uses thereof

<130>  F226 - 191Ext

<160>  26    

<170>  PatentIn version 3.4

<210>  1
<211>  10
<212>  DNA
<213>  Artificial

<220>
<223>  Consensus for attCr site


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  comprises at least 4 nucleotides of which the third from last 
       nucleotide is A and is complementary to N9

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  if present comprises no more than 2 nucleotides

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  comprises 6 to 10 nucleotides and is non-complimentary to N7

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  comprises 9 to 30 nucleotides and is complimentary to N6

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  comprises 3 to 50 nucleotides

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  comprises 9 to 30 nucleotides and is complimentary to N4 which 
       comprises at least one nucleotide which forms a extra helical 
       base pair when N4 and N6 form a double helical strand

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  comprises 6 to 10 nucleotides and is non-complimentary to N3

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  if present comprises no more than 2 nucleotides

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  comprises at least 4 nucleotides of which the second from last 
       nucleotide is T and is complementary to N1

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  any nucleotide if present

<400>  1
nnnnnnnnnn                                                            10


<210>  2
<211>  13
<212>  DNA
<213>  Artificial

<220>
<223>  attCr specific consensus


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  comprises 6 to 10 nucleotides and is non-complimentary to N9

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  comprises 9 to 30 nucleotides and is complimentary to N8

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  comprises 3 to 50 nucleotides

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  comprises 9 to 30 nucleotides and is complimentary to N6 which 
       comprises at least one nucleotide which forms a extra helical 
       base pair when N6 and N8 form a double helical strand

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  comprises 6 to 10 nucleotides and is non-complimentary to N5

<400>  2
taacnnnnng tta                                                        13


<210>  3
<211>  67
<212>  DNA
<213>  Artificial

<220>
<223>  r0-top

<400>  3
aattcattat aacggaggtt acccatggat tcgagttcct cgaaccatgg taaagagtgt     60

tataatg                                                               67


<210>  4
<211>  67
<212>  DNA
<213>  Artificial

<220>
<223>  r0-bottom

<400>  4
gatccattat aacactcttt accatggttc gaggaactcg aatccatggg taacctccgt     60

tataatg                                                               67


<210>  5
<211>  67
<212>  DNA
<213>  Artificial

<220>
<223>  r1-top

<400>  5
aattccgtct aactcatcgc gcgtgaataa acctcttgga ggttattcac cgcaaaatgt     60

tagacgg                                                               67


<210>  6
<211>  67
<212>  DNA
<213>  Artificial

<220>
<223>  r1-bottom

<400>  6
gatcccgtct aacattttgc ggtgaataac ctccaagagg tttattcacg cgcgatgagt     60

tagacgg                                                               67


<210>  7
<211>  67
<212>  DNA
<213>  Artificial

<220>
<223>  r2-top

<400>  7
aattcagggt aacgctacgc gcagtgccaa gcatctatga tgctggcact cgcctcttgt     60

taccctg                                                               67


<210>  8
<211>  67
<212>  DNA
<213>  Artificial

<220>
<223>  r2-bottom

<400>  8
gatccagggt aacaagaggc gagtgccagc atcatagatg cttggcactg cgcgtagcgt     60

taccctg                                                               67


<210>  9
<211>  67
<212>  DNA
<213>  Artificial

<220>
<223>  r3-top

<400>  9
aattctctct aacctgctac tctatagtac agtaaggttt actgactata agtcgggtgt     60

tagagag                                                               67


<210>  10
<211>  67
<212>  DNA
<213>  Artificial

<220>
<223>  r3-bottom

<400>  10
gatcctctct aacacccgac ttatagtcag taaaccttac tgtactatag agtagcaggt     60

tagagag                                                               67


<210>  11
<211>  67
<212>  DNA
<213>  Artificial

<220>
<223>  r4-top

<400>  11
aattcagcgt aacaattgtg actgattaag caagcccagc ttgctaatca tcaagcacgt     60

tacgctg                                                               67


<210>  12
<211>  67
<212>  DNA
<213>  Artificial

<220>
<223>  r4-bottom

<400>  12
gatccagcgt aacgtgcttg atgattagca agctgggctt gcttaatcag tcacaattgt     60

tacgctg                                                               67


<210>  13
<211>  67
<212>  DNA
<213>  Artificial

<220>
<223>  r5-top

<400>  13
aattctttct aactccgcca acccggagaa gcgtggccca cgctctccgg ttgatattgt     60

tagaaag                                                               67


<210>  14
<211>  67
<212>  DNA
<213>  Artificial

<220>
<223>  r5-bottom

<400>  14
gatcctttct aacaatatca accggagagc gtgggccacg cttctccggg ttggcggagt     60

tagaaag                                                               67


<210>  15
<211>  67
<212>  DNA
<213>  Artificial

<220>
<223>  r6-top

<400>  15
aattcgcctt aacaatacag gctatgttat ttggtcggac caaaaacata cctagtaggt     60

taaggcg                                                               67


<210>  16
<211>  67
<212>  DNA
<213>  Artificial

<220>
<223>  r6-bottom

<400>  16
gatccgcctt aacctactag gtatgttttt ggtccgacca aataacatag cctgtattgt     60

taaggcg                                                               67


<210>  17
<211>  67
<212>  DNA
<213>  Artificial

<220>
<223>  r7-top

<400>  17
aattcatctt aactgcttac acccgggcaa cctttcctaa aggtgcccgg tgtggcttgt     60

taagatg                                                               67


<210>  18
<211>  67
<212>  DNA
<213>  Artificial

<220>
<223>  r7-bottom

<400>  18
gatccatctt aacaagccac accgggcacc tttaggaaag gttgcccggg tgtaagcagt     60

taagatg                                                               67


<210>  19
<211>  82
<212>  DNA
<213>  Artificial

<220>
<223>  rdmAttC0


<220>
<221>  misc_feature
<222>  (23)..(26)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (56)..(59)
<223>  n is a, c, g, or t

<400>  19
gatcggatcc attataacac tcnnnnccat ggttcgacga actdgaatcc atgggnnnnc     60

tccgttataa tgaattcacc ag                                              82


<210>  20
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  primerrdmAttC0

<400>  20
ctggtgaatt cattataacg gag                                             23


<210>  21
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  MRV

<400>  21
agcggataac aatttcacac agga                                            24


<210>  22
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  MRVD2

<400>  22
ttctgctgac gcaccggtg                                                  19


<210>  23
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  SWbegin

<400>  23
ccgtcacagg tatttattcg gcg                                             23


<210>  24
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  SWend

<400>  24
cctcactaaa gggaacaaaa gctg                                            24


<210>  25
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  BamHI-pSUlib-F

<400>  25
tttggatcca ctagtagcgg ccgctgcag                                       29


<210>  26
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  attI0

<400>  26
tttggatcca ttataacttt gttttagggc gac                                  33


