               SEQUENCE LISTING

<110> Charite - Universitaetsmedizin Berlin 
      ICI immunochemical intelligence GmbH

<120> IDENTIFICATION OF MODULATORS OF BINDING PROPERTIES OF ANTIBODIES REACTIVE WITH A MEMBER OF THE INSULIN RECEPTOR FAMILY

<130> DG011022-P

<150> EP12156930.5
<151> 2012-02-24

<160> 20

<170> BiSSAP 1.2

<210> 1
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..33
<223> /organism="Artificial Sequence"
      /note="primer P1"
      /mol_type="unassigned DNA"

<400> 1
gcggaattcg tcaccgacgc caaaaacata aag                                  33


<210> 2
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..34
<223> /organism="Artificial Sequence"
      /note="Primer P2"
      /mol_type="unassigned DNA"

<400> 2
acgggattct tacacggcga tctttccgcc cttc                                 34


<210> 3
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..35
<223> /organism="Artificial Sequence"
      /note="Primer P3"
      /mol_type="unassigned DNA"

<400> 3
acggcggccg catgaagtct ggctccggag gaggg                                35


<210> 4
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..33
<223> /organism="Artificial Sequence"
      /note="Primer P4"
      /mol_type="unassigned DNA"

<400> 4
acggaattcg caggtcgaag actggggcag cgg                                  33


<210> 5
<211> 1653
<212> DNA
<213> Photinus pyralis

<220> 
<221> source
<222> 1..1653
<223> /organism="Photinus pyralis"
      /note="cds"
      /mol_type="unassigned DNA"

<400> 5
gtcaccgacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga      60

accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt     120

gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc     180

gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta     240

tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt     300

gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt     360

tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa     420

aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga     480

tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat     540

tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga     600

tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg     660

catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt     720

gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt     780

cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac     840

aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg     900

attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct     960

aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat    1020

gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc    1080

gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa    1140

acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt    1200

tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct    1260

ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct    1320

ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa    1380

caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt    1440

cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat    1500

tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac    1560

gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata    1620

aaggccaaga agggcggaaa gatcgccgtg taa                                 1653


<210> 6
<211> 550
<212> PRT
<213> Photinus pyralis


<400> 6
Val Thr Asp Ala Lys Asn Ile Lys Lys Gly Pro Ala Pro Phe Tyr Pro 
1               5                   10                  15      
Leu Glu Asp Gly Thr Ala Gly Glu Gln Leu His Lys Ala Met Lys Arg 
            20                  25                  30          
Tyr Ala Leu Val Pro Gly Thr Ile Ala Phe Thr Asp Ala His Ile Glu 
        35                  40                  45              
Val Asp Ile Thr Tyr Ala Glu Tyr Phe Glu Met Ser Val Arg Leu Ala 
    50                  55                  60                  
Glu Ala Met Lys Arg Tyr Gly Leu Asn Thr Asn His Arg Ile Val Val 
65                  70                  75                  80  
Cys Ser Glu Asn Ser Leu Gln Phe Phe Met Pro Val Leu Gly Ala Leu 
                85                  90                  95      
Phe Ile Gly Val Ala Val Ala Pro Ala Asn Asp Ile Tyr Asn Glu Arg 
            100                 105                 110         
Glu Leu Leu Asn Ser Met Gly Ile Ser Gln Pro Thr Val Val Phe Val 
        115                 120                 125             
Ser Lys Lys Gly Leu Gln Lys Ile Leu Asn Val Gln Lys Lys Leu Pro 
    130                 135                 140                 
Ile Ile Gln Lys Ile Ile Ile Met Asp Ser Lys Thr Asp Tyr Gln Gly 
145                 150                 155                 160 
Phe Gln Ser Met Tyr Thr Phe Val Thr Ser His Leu Pro Pro Gly Phe 
                165                 170                 175     
Asn Glu Tyr Asp Phe Val Pro Glu Ser Phe Asp Arg Asp Lys Thr Ile 
            180                 185                 190         
Ala Leu Ile Met Asn Ser Ser Gly Ser Thr Gly Leu Pro Lys Gly Val 
        195                 200                 205             
Ala Leu Pro His Arg Thr Ala Cys Val Arg Phe Ser His Ala Arg Asp 
    210                 215                 220                 
Pro Ile Phe Gly Asn Gln Ile Ile Pro Asp Thr Ala Ile Leu Ser Val 
225                 230                 235                 240 
Val Pro Phe His His Gly Phe Gly Met Phe Thr Thr Leu Gly Tyr Leu 
                245                 250                 255     
Ile Cys Gly Phe Arg Val Val Leu Met Tyr Arg Phe Glu Glu Glu Leu 
            260                 265                 270         
Phe Leu Arg Ser Leu Gln Asp Tyr Lys Ile Gln Ser Ala Leu Leu Val 
        275                 280                 285             
Pro Thr Leu Phe Ser Phe Phe Ala Lys Ser Thr Leu Ile Asp Lys Tyr 
    290                 295                 300                 
Asp Leu Ser Asn Leu His Glu Ile Ala Ser Gly Gly Ala Pro Leu Ser 
305                 310                 315                 320 
Lys Glu Val Gly Glu Ala Val Ala Lys Arg Phe His Leu Pro Gly Ile 
                325                 330                 335     
Arg Gln Gly Tyr Gly Leu Thr Glu Thr Thr Ser Ala Ile Leu Ile Thr 
            340                 345                 350         
Pro Glu Gly Asp Asp Lys Pro Gly Ala Val Gly Lys Val Val Pro Phe 
        355                 360                 365             
Phe Glu Ala Lys Val Val Asp Leu Asp Thr Gly Lys Thr Leu Gly Val 
    370                 375                 380                 
Asn Gln Arg Gly Glu Leu Cys Val Arg Gly Pro Met Ile Met Ser Gly 
385                 390                 395                 400 
Tyr Val Asn Asn Pro Glu Ala Thr Asn Ala Leu Ile Asp Lys Asp Gly 
                405                 410                 415     
Trp Leu His Ser Gly Asp Ile Ala Tyr Trp Asp Glu Asp Glu His Phe 
            420                 425                 430         
Phe Ile Val Asp Arg Leu Lys Ser Leu Ile Lys Tyr Lys Gly Tyr Gln 
        435                 440                 445             
Val Ala Pro Ala Glu Leu Glu Ser Ile Leu Leu Gln His Pro Asn Ile 
    450                 455                 460                 
Phe Asp Ala Gly Val Ala Gly Leu Pro Asp Asp Asp Ala Gly Glu Leu 
465                 470                 475                 480 
Pro Ala Ala Val Val Val Leu Glu His Gly Lys Thr Met Thr Glu Lys 
                485                 490                 495     
Glu Ile Val Asp Tyr Val Ala Ser Gln Val Thr Thr Ala Lys Lys Leu 
            500                 505                 510         
Arg Gly Gly Val Val Phe Val Asp Glu Val Pro Lys Gly Leu Thr Gly 
        515                 520                 525             
Lys Leu Asp Ala Arg Lys Ile Arg Glu Ile Leu Ile Lys Ala Lys Lys 
    530                 535                 540                 
Gly Gly Lys Ile Ala Val 
545                 550 

<210> 7
<211> 4104
<212> DNA
<213> Homo sapiens

<220> 
<221> source
<222> 1..4104
<223> /organism="Homo sapiens"
      /note="IGF1R"
      /mol_type="unassigned DNA"

<400> 7
atgaagtctg gctccggagg agggtccccg acctcgctgt gggggctcct gtttctctcc       60

gccgcgctct cgctctggcc gacgagtgga gaaatctgcg ggccaggcat cgacatccgc      120

aacgactatc agcagctgaa gcgcctggag aactgcacgg tgatcgaggg ctacctccac      180

atcctgctca tctccaaggc cgaggactac cgcagctacc gcttccccaa gctcacggtc      240

attaccgagt acttgctgct gttccgagtg gctggcctcg agagcctcgg agacctcttc      300

cccaacctca cggtcatccg cggctggaaa ctcttctaca actacgccct ggtcatcttc      360

gagatgacca atctcaagga tattgggctt tacaacctga ggaacattac tcggggggcc      420

atcaggattg agaaaaatgc tgacctctgt tacctctcca ctgtggactg gtccctgatc      480

ctggatgcgg tgtccaataa ctacattgtg gggaataagc ccccaaagga atgtggggac      540

ctgtgtccag ggaccatgga ggagaagccg atgtgtgaga agaccaccat caacaatgag      600

tacaactacc gctgctggac cacaaaccgc tgccagaaaa tgtgcccaag cacgtgtggg      660

aagcgggcgt gcaccgagaa caatgagtgc tgccaccccg agtgcctggg cagctgcagc      720

gcgcctgaca acgacacggc ctgtgtagct tgccgccact actactatgc cggtgtctgt      780

gtgcctgcct gcccgcccaa cacctacagg tttgagggct ggcgctgtgt ggaccgtgac      840

ttctgcgcca acatcctcag cgccgagagc agcgactccg aggggtttgt gatccacgac      900

ggcgagtgca tgcaggagtg cccctcgggc ttcatccgca acggcagcca gagcatgtac      960

tgcatccctt gtgaaggtcc ttgcccgaag gtctgtgagg aagaaaagaa aacaaagacc     1020

attgattctg ttacttctgc tcagatgctc caaggatgca ccatcttcaa gggcaatttg     1080

ctcattaaca tccgacgggg gaataacatt gcttcagagc tggagaactt catggggctc     1140

atcgaggtgg tgacgggcta cgtgaagatc cgccattctc atgccttggt ctccttgtcc     1200

ttcctaaaaa accttcgcct catcctagga gaggagcagc tagaagggaa ttactccttc     1260

tacgtcctcg acaaccagaa cttgcagcaa ctgtgggact gggaccaccg caacctgacc     1320

atcaaagcag ggaaaatgta ctttgctttc aatcccaaat tatgtgtttc cgaaatttac     1380

cgcatggagg aagtgacggg gactaaaggg cgccaaagca aaggggacat aaacaccagg     1440

aacaacgggg agagagcctc ctgtgaaagt gacgtcctgc atttcacctc caccaccacg     1500

tcgaagaatc gcatcatcat aacctggcac cggtaccggc cccctgacta cagggatctc     1560

atcagcttca ccgtttacta caaggaagca ccctttaaga atgtcacaga gtatgatggg     1620

caggatgcct gcggctccaa cagctggaac atggtggacg tggacctccc gcccaacaag     1680

gacgtggagc ccggcatctt actacatggg ctgaagccct ggactcagta cgccgtttac     1740

gtcaaggctg tgaccctcac catggtggag aacgaccata tccgtggggc caagagtgag     1800

atcttgtaca ttcgcaccaa tgcttcagtt ccttccattc ccttggacgt tctttcagca     1860

tcgaactcct cttctcagtt aatcgtgaag tggaaccctc cctctctgcc caacggcaac     1920

ctgagttact acattgtgcg ctggcagcgg cagcctcagg acggctacct ttaccggcac     1980

aattactgct ccaaagacaa aatccccatc aggaagtatg ccgacggcac catcgacatt     2040

gaggaggtca cagagaaccc caagactgag gtgtgtggtg gggagaaagg gccttgctgc     2100

gcctgcccca aaactgaagc cgagaagcag gccgagaagg aggaggctga ataccgcaaa     2160

gtctttgaga atttcctgca caactccatc ttcgtgccca gacctgaaag gaagcggaga     2220

gatgtcatgc aagtggccaa caccaccatg tccagccgaa gcaggaacac cacggccgca     2280

gacacctaca acatcaccga cccggaagag ctggagacag agtacccttt ctttgagagc     2340

agagtggata acaaggagag aactgtcatt tctaaccttc ggcctttcac attgtaccgc     2400

atcgatatcc acagctgcaa ccacgaggct gagaagctgg gctgcagcgc ctccaacttc     2460

gtctttgcaa ggactatgcc cgcagaagga gcagatgaca ttcctgggcc agtgacctgg     2520

gagccaaggc ctgaaaactc catcttttta aagtggccgg aacctgagaa tcccaatgga     2580

ttgattctaa tgtatgaaat aaaatacgga tcacaagttg aggatcagcg agaatgtgtg     2640

tccagacagg aatacaggaa gtatggaggg gccaagctaa accggctaaa cccggggaac     2700

tacacagccc ggattcaggc cacatctctc tctgggaatg ggtcgtggac agatcctgtg     2760

ttcttctatg tccaggccaa aacaggatat gaaaacttca tccatctgat catcgctctg     2820

cccgtcgctg tcctgttgat cgtgggaggg ttggtgatta tgctgtacgt cttccataga     2880

aagagaaata acagcaggct ggggaatgga gtgctgtatg cctctgtgaa cccggagtac     2940

ttcagcgctg ctgatgtgta cgttcctgat gagtgggagg tggctcggga gaagatcacc     3000

atgagccggg aacttgggca ggggtcgttt gggatggtct atgaaggagt tgccaagggt     3060

gtggtgaaag atgaacctga aaccagagtg gccattaaaa cagtgaacga ggccgcaagc     3120

atgcgtgaga ggattgagtt tctcaacgaa gcttctgtga tgaaggagtt caattgtcac     3180

catgtggtgc gattgctggg tgtggtgtcc caaggccagc caacactggt catcatggaa     3240

ctgatgacac ggggcgatct caaaagttat ctccggtctc tgaggccaga aatggagaat     3300

aatccagtcc tagcacctcc aagcctgagc aagatgattc agatggccgg agagattgca     3360

gacggcatgg catacctcaa cgccaataag ttcgtccaca gagaccttgc tgcccggaat     3420

tgcatggtag ccgaagattt cacagtcaaa atcggagatt ttggtatgac gcgagatatc     3480

tatgagacag actattaccg gaaaggaggg aaagggctgc tgcccgtgcg ctggatgtct     3540

cctgagtccc tcaaggatgg agtcttcacc acttactcgg acgtctggtc cttcggggtc     3600

gtcctctggg agatcgccac actggccgag cagccctacc agggcttgtc caacgagcaa     3660

gtccttcgct tcgtcatgga gggcggcctt ctggacaagc cagacaactg tcctgacatg     3720

ctgtttgaac tgatgcgcat gtgctggcag tataacccca agatgaggcc ttccttcctg     3780

gagatcatca gcagcatcaa agaggagatg gagcctggct tccgggaggt ctccttctac     3840

tacagcgagg agaacaagct gcccgagccg gaggagctgg acctggagcc agagaacatg     3900

gagagcgtcc ccctggaccc ctcggcctcc tcgtcctccc tgccactgcc cgacagacac     3960

tcaggacaca aggccgagaa cggccccggc cctggggtgc tggtcctccg cgccagcttc     4020

gacgagagac agccttacgc ccacatgaac gggggccgca agaacgagcg ggccttgccg     4080

ctgccccagt cttcgacctg ctga                                            4104


<210> 8
<211> 1367
<212> PRT
<213> Homo sapiens

<220> 
<223> IGF1R

<400> 8
Met Lys Ser Gly Ser Gly Gly Gly Ser Pro Thr Ser Leu Trp Gly Leu 
1               5                   10                  15      
Leu Phe Leu Ser Ala Ala Leu Ser Leu Trp Pro Thr Ser Gly Glu Ile 
            20                  25                  30          
Cys Gly Pro Gly Ile Asp Ile Arg Asn Asp Tyr Gln Gln Leu Lys Arg 
        35                  40                  45              
Leu Glu Asn Cys Thr Val Ile Glu Gly Tyr Leu His Ile Leu Leu Ile 
    50                  55                  60                  
Ser Lys Ala Glu Asp Tyr Arg Ser Tyr Arg Phe Pro Lys Leu Thr Val 
65                  70                  75                  80  
Ile Thr Glu Tyr Leu Leu Leu Phe Arg Val Ala Gly Leu Glu Ser Leu 
                85                  90                  95      
Gly Asp Leu Phe Pro Asn Leu Thr Val Ile Arg Gly Trp Lys Leu Phe 
            100                 105                 110         
Tyr Asn Tyr Ala Leu Val Ile Phe Glu Met Thr Asn Leu Lys Asp Ile 
        115                 120                 125             
Gly Leu Tyr Asn Leu Arg Asn Ile Thr Arg Gly Ala Ile Arg Ile Glu 
    130                 135                 140                 
Lys Asn Ala Asp Leu Cys Tyr Leu Ser Thr Val Asp Trp Ser Leu Ile 
145                 150                 155                 160 
Leu Asp Ala Val Ser Asn Asn Tyr Ile Val Gly Asn Lys Pro Pro Lys 
                165                 170                 175     
Glu Cys Gly Asp Leu Cys Pro Gly Thr Met Glu Glu Lys Pro Met Cys 
            180                 185                 190         
Glu Lys Thr Thr Ile Asn Asn Glu Tyr Asn Tyr Arg Cys Trp Thr Thr 
        195                 200                 205             
Asn Arg Cys Gln Lys Met Cys Pro Ser Thr Cys Gly Lys Arg Ala Cys 
    210                 215                 220                 
Thr Glu Asn Asn Glu Cys Cys His Pro Glu Cys Leu Gly Ser Cys Ser 
225                 230                 235                 240 
Ala Pro Asp Asn Asp Thr Ala Cys Val Ala Cys Arg His Tyr Tyr Tyr 
                245                 250                 255     
Ala Gly Val Cys Val Pro Ala Cys Pro Pro Asn Thr Tyr Arg Phe Glu 
            260                 265                 270         
Gly Trp Arg Cys Val Asp Arg Asp Phe Cys Ala Asn Ile Leu Ser Ala 
        275                 280                 285             
Glu Ser Ser Asp Ser Glu Gly Phe Val Ile His Asp Gly Glu Cys Met 
    290                 295                 300                 
Gln Glu Cys Pro Ser Gly Phe Ile Arg Asn Gly Ser Gln Ser Met Tyr 
305                 310                 315                 320 
Cys Ile Pro Cys Glu Gly Pro Cys Pro Lys Val Cys Glu Glu Glu Lys 
                325                 330                 335     
Lys Thr Lys Thr Ile Asp Ser Val Thr Ser Ala Gln Met Leu Gln Gly 
            340                 345                 350         
Cys Thr Ile Phe Lys Gly Asn Leu Leu Ile Asn Ile Arg Arg Gly Asn 
        355                 360                 365             
Asn Ile Ala Ser Glu Leu Glu Asn Phe Met Gly Leu Ile Glu Val Val 
    370                 375                 380                 
Thr Gly Tyr Val Lys Ile Arg His Ser His Ala Leu Val Ser Leu Ser 
385                 390                 395                 400 
Phe Leu Lys Asn Leu Arg Leu Ile Leu Gly Glu Glu Gln Leu Glu Gly 
                405                 410                 415     
Asn Tyr Ser Phe Tyr Val Leu Asp Asn Gln Asn Leu Gln Gln Leu Trp 
            420                 425                 430         
Asp Trp Asp His Arg Asn Leu Thr Ile Lys Ala Gly Lys Met Tyr Phe 
        435                 440                 445             
Ala Phe Asn Pro Lys Leu Cys Val Ser Glu Ile Tyr Arg Met Glu Glu 
    450                 455                 460                 
Val Thr Gly Thr Lys Gly Arg Gln Ser Lys Gly Asp Ile Asn Thr Arg 
465                 470                 475                 480 
Asn Asn Gly Glu Arg Ala Ser Cys Glu Ser Asp Val Leu His Phe Thr 
                485                 490                 495     
Ser Thr Thr Thr Ser Lys Asn Arg Ile Ile Ile Thr Trp His Arg Tyr 
            500                 505                 510         
Arg Pro Pro Asp Tyr Arg Asp Leu Ile Ser Phe Thr Val Tyr Tyr Lys 
        515                 520                 525             
Glu Ala Pro Phe Lys Asn Val Thr Glu Tyr Asp Gly Gln Asp Ala Cys 
    530                 535                 540                 
Gly Ser Asn Ser Trp Asn Met Val Asp Val Asp Leu Pro Pro Asn Lys 
545                 550                 555                 560 
Asp Val Glu Pro Gly Ile Leu Leu His Gly Leu Lys Pro Trp Thr Gln 
                565                 570                 575     
Tyr Ala Val Tyr Val Lys Ala Val Thr Leu Thr Met Val Glu Asn Asp 
            580                 585                 590         
His Ile Arg Gly Ala Lys Ser Glu Ile Leu Tyr Ile Arg Thr Asn Ala 
        595                 600                 605             
Ser Val Pro Ser Ile Pro Leu Asp Val Leu Ser Ala Ser Asn Ser Ser 
    610                 615                 620                 
Ser Gln Leu Ile Val Lys Trp Asn Pro Pro Ser Leu Pro Asn Gly Asn 
625                 630                 635                 640 
Leu Ser Tyr Tyr Ile Val Arg Trp Gln Arg Gln Pro Gln Asp Gly Tyr 
                645                 650                 655     
Leu Tyr Arg His Asn Tyr Cys Ser Lys Asp Lys Ile Pro Ile Arg Lys 
            660                 665                 670         
Tyr Ala Asp Gly Thr Ile Asp Ile Glu Glu Val Thr Glu Asn Pro Lys 
        675                 680                 685             
Thr Glu Val Cys Gly Gly Glu Lys Gly Pro Cys Cys Ala Cys Pro Lys 
    690                 695                 700                 
Thr Glu Ala Glu Lys Gln Ala Glu Lys Glu Glu Ala Glu Tyr Arg Lys 
705                 710                 715                 720 
Val Phe Glu Asn Phe Leu His Asn Ser Ile Phe Val Pro Arg Pro Glu 
                725                 730                 735     
Arg Lys Arg Arg Asp Val Met Gln Val Ala Asn Thr Thr Met Ser Ser 
            740                 745                 750         
Arg Ser Arg Asn Thr Thr Ala Ala Asp Thr Tyr Asn Ile Thr Asp Pro 
        755                 760                 765             
Glu Glu Leu Glu Thr Glu Tyr Pro Phe Phe Glu Ser Arg Val Asp Asn 
    770                 775                 780                 
Lys Glu Arg Thr Val Ile Ser Asn Leu Arg Pro Phe Thr Leu Tyr Arg 
785                 790                 795                 800 
Ile Asp Ile His Ser Cys Asn His Glu Ala Glu Lys Leu Gly Cys Ser 
                805                 810                 815     
Ala Ser Asn Phe Val Phe Ala Arg Thr Met Pro Ala Glu Gly Ala Asp 
            820                 825                 830         
Asp Ile Pro Gly Pro Val Thr Trp Glu Pro Arg Pro Glu Asn Ser Ile 
        835                 840                 845             
Phe Leu Lys Trp Pro Glu Pro Glu Asn Pro Asn Gly Leu Ile Leu Met 
    850                 855                 860                 
Tyr Glu Ile Lys Tyr Gly Ser Gln Val Glu Asp Gln Arg Glu Cys Val 
865                 870                 875                 880 
Ser Arg Gln Glu Tyr Arg Lys Tyr Gly Gly Ala Lys Leu Asn Arg Leu 
                885                 890                 895     
Asn Pro Gly Asn Tyr Thr Ala Arg Ile Gln Ala Thr Ser Leu Ser Gly 
            900                 905                 910         
Asn Gly Ser Trp Thr Asp Pro Val Phe Phe Tyr Val Gln Ala Lys Thr 
        915                 920                 925             
Gly Tyr Glu Asn Phe Ile His Leu Ile Ile Ala Leu Pro Val Ala Val 
    930                 935                 940                 
Leu Leu Ile Val Gly Gly Leu Val Ile Met Leu Tyr Val Phe His Arg 
945                 950                 955                 960 
Lys Arg Asn Asn Ser Arg Leu Gly Asn Gly Val Leu Tyr Ala Ser Val 
                965                 970                 975     
Asn Pro Glu Tyr Phe Ser Ala Ala Asp Val Tyr Val Pro Asp Glu Trp 
            980                 985                 990         
Glu Val Ala Arg Glu Lys Ile Thr Met Ser Arg Glu Leu Gly Gln Gly 
        995                 1000                1005            
Ser Phe Gly Met Val Tyr Glu Gly Val Ala Lys Gly Val Val Lys Asp 
    1010                1015                1020                
Glu Pro Glu Thr Arg Val Ala Ile Lys Thr Val Asn Glu Ala Ala Ser 
1025                1030                1035                1040
Met Arg Glu Arg Ile Glu Phe Leu Asn Glu Ala Ser Val Met Lys Glu 
                1045                1050                1055    
Phe Asn Cys His His Val Val Arg Leu Leu Gly Val Val Ser Gln Gly 
            1060                1065                1070        
Gln Pro Thr Leu Val Ile Met Glu Leu Met Thr Arg Gly Asp Leu Lys 
        1075                1080                1085            
Ser Tyr Leu Arg Ser Leu Arg Pro Glu Met Glu Asn Asn Pro Val Leu 
    1090                1095                1100                
Ala Pro Pro Ser Leu Ser Lys Met Ile Gln Met Ala Gly Glu Ile Ala 
1105                1110                1115                1120
Asp Gly Met Ala Tyr Leu Asn Ala Asn Lys Phe Val His Arg Asp Leu 
                1125                1130                1135    
Ala Ala Arg Asn Cys Met Val Ala Glu Asp Phe Thr Val Lys Ile Gly 
            1140                1145                1150        
Asp Phe Gly Met Thr Arg Asp Ile Tyr Glu Thr Asp Tyr Tyr Arg Lys 
        1155                1160                1165            
Gly Gly Lys Gly Leu Leu Pro Val Arg Trp Met Ser Pro Glu Ser Leu 
    1170                1175                1180                
Lys Asp Gly Val Phe Thr Thr Tyr Ser Asp Val Trp Ser Phe Gly Val 
1185                1190                1195                1200
Val Leu Trp Glu Ile Ala Thr Leu Ala Glu Gln Pro Tyr Gln Gly Leu 
                1205                1210                1215    
Ser Asn Glu Gln Val Leu Arg Phe Val Met Glu Gly Gly Leu Leu Asp 
            1220                1225                1230        
Lys Pro Asp Asn Cys Pro Asp Met Leu Phe Glu Leu Met Arg Met Cys 
        1235                1240                1245            
Trp Gln Tyr Asn Pro Lys Met Arg Pro Ser Phe Leu Glu Ile Ile Ser 
    1250                1255                1260                
Ser Ile Lys Glu Glu Met Glu Pro Gly Phe Arg Glu Val Ser Phe Tyr 
1265                1270                1275                1280
Tyr Ser Glu Glu Asn Lys Leu Pro Glu Pro Glu Glu Leu Asp Leu Glu 
                1285                1290                1295    
Pro Glu Asn Met Glu Ser Val Pro Leu Asp Pro Ser Ala Ser Ser Ser 
            1300                1305                1310        
Ser Leu Pro Leu Pro Asp Arg His Ser Gly His Lys Ala Glu Asn Gly 
        1315                1320                1325            
Pro Gly Pro Gly Val Leu Val Leu Arg Ala Ser Phe Asp Glu Arg Gln 
    1330                1335                1340                
Pro Tyr Ala His Met Asn Gly Gly Arg Lys Asn Glu Arg Ala Leu Pro 
1345                1350                1355                1360
Leu Pro Gln Ser Ser Thr Cys 
                1365        

<210> 9
<211> 5760
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..5760
<223> /organism="Artificial Sequence"
      /note="hIGF1R-luc fusion"
      /mol_type="unassigned DNA"

<400> 9
atgaagtctg gctccggagg agggtccccg acctcgctgt gggggctcct gtttctctcc       60

gccgcgctct cgctctggcc gacgagtgga gaaatctgcg ggccaggcat cgacatccgc      120

aacgactatc agcagctgaa gcgcctggag aactgcacgg tgatcgaggg ctacctccac      180

atcctgctca tctccaaggc cgaggactac cgcagctacc gcttccccaa gctcacggtc      240

attaccgagt acttgctgct gttccgagtg gctggcctcg agagcctcgg agacctcttc      300

cccaacctca cggtcatccg cggctggaaa ctcttctaca actacgccct ggtcatcttc      360

gagatgacca atctcaagga tattgggctt tacaacctga ggaacattac tcggggggcc      420

atcaggattg agaaaaatgc tgacctctgt tacctctcca ctgtggactg gtccctgatc      480

ctggatgcgg tgtccaataa ctacattgtg gggaataagc ccccaaagga atgtggggac      540

ctgtgtccag ggaccatgga ggagaagccg atgtgtgaga agaccaccat caacaatgag      600

tacaactacc gctgctggac cacaaaccgc tgccagaaaa tgtgcccaag cacgtgtggg      660

aagcgggcgt gcaccgagaa caatgagtgc tgccaccccg agtgcctggg cagctgcagc      720

gcgcctgaca acgacacggc ctgtgtagct tgccgccact actactatgc cggtgtctgt      780

gtgcctgcct gcccgcccaa cacctacagg tttgagggct ggcgctgtgt ggaccgtgac      840

ttctgcgcca acatcctcag cgccgagagc agcgactccg aggggtttgt gatccacgac      900

ggcgagtgca tgcaggagtg cccctcgggc ttcatccgca acggcagcca gagcatgtac      960

tgcatccctt gtgaaggtcc ttgcccgaag gtctgtgagg aagaaaagaa aacaaagacc     1020

attgattctg ttacttctgc tcagatgctc caaggatgca ccatcttcaa gggcaatttg     1080

ctcattaaca tccgacgggg gaataacatt gcttcagagc tggagaactt catggggctc     1140

atcgaggtgg tgacgggcta cgtgaagatc cgccattctc atgccttggt ctccttgtcc     1200

ttcctaaaaa accttcgcct catcctagga gaggagcagc tagaagggaa ttactccttc     1260

tacgtcctcg acaaccagaa cttgcagcaa ctgtgggact gggaccaccg caacctgacc     1320

atcaaagcag ggaaaatgta ctttgctttc aatcccaaat tatgtgtttc cgaaatttac     1380

cgcatggagg aagtgacggg gactaaaggg cgccaaagca aaggggacat aaacaccagg     1440

aacaacgggg agagagcctc ctgtgaaagt gacgtcctgc atttcacctc caccaccacg     1500

tcgaagaatc gcatcatcat aacctggcac cggtaccggc cccctgacta cagggatctc     1560

atcagcttca ccgtttacta caaggaagca ccctttaaga atgtcacaga gtatgatggg     1620

caggatgcct gcggctccaa cagctggaac atggtggacg tggacctccc gcccaacaag     1680

gacgtggagc ccggcatctt actacatggg ctgaagccct ggactcagta cgccgtttac     1740

gtcaaggctg tgaccctcac catggtggag aacgaccata tccgtggggc caagagtgag     1800

atcttgtaca ttcgcaccaa tgcttcagtt ccttccattc ccttggacgt tctttcagca     1860

tcgaactcct cttctcagtt aatcgtgaag tggaaccctc cctctctgcc caacggcaac     1920

ctgagttact acattgtgcg ctggcagcgg cagcctcagg acggctacct ttaccggcac     1980

aattactgct ccaaagacaa aatccccatc aggaagtatg ccgacggcac catcgacatt     2040

gaggaggtca cagagaaccc caagactgag gtgtgtggtg gggagaaagg gccttgctgc     2100

gcctgcccca aaactgaagc cgagaagcag gccgagaagg aggaggctga ataccgcaaa     2160

gtctttgaga atttcctgca caactccatc ttcgtgccca gacctgaaag gaagcggaga     2220

gatgtcatgc aagtggccaa caccaccatg tccagccgaa gcaggaacac cacggccgca     2280

gacacctaca acatcaccga cccggaagag ctggagacag agtacccttt ctttgagagc     2340

agagtggata acaaggagag aactgtcatt tctaaccttc ggcctttcac attgtaccgc     2400

atcgatatcc acagctgcaa ccacgaggct gagaagctgg gctgcagcgc ctccaacttc     2460

gtctttgcaa ggactatgcc cgcagaagga gcagatgaca ttcctgggcc agtgacctgg     2520

gagccaaggc ctgaaaactc catcttttta aagtggccgg aacctgagaa tcccaatgga     2580

ttgattctaa tgtatgaaat aaaatacgga tcacaagttg aggatcagcg agaatgtgtg     2640

tccagacagg aatacaggaa gtatggaggg gccaagctaa accggctaaa cccggggaac     2700

tacacagccc ggattcaggc cacatctctc tctgggaatg ggtcgtggac agatcctgtg     2760

ttcttctatg tccaggccaa aacaggatat gaaaacttca tccatctgat catcgctctg     2820

cccgtcgctg tcctgttgat cgtgggaggg ttggtgatta tgctgtacgt cttccataga     2880

aagagaaata acagcaggct ggggaatgga gtgctgtatg cctctgtgaa cccggagtac     2940

ttcagcgctg ctgatgtgta cgttcctgat gagtgggagg tggctcggga gaagatcacc     3000

atgagccggg aacttgggca ggggtcgttt gggatggtct atgaaggagt tgccaagggt     3060

gtggtgaaag atgaacctga aaccagagtg gccattaaaa cagtgaacga ggccgcaagc     3120

atgcgtgaga ggattgagtt tctcaacgaa gcttctgtga tgaaggagtt caattgtcac     3180

catgtggtgc gattgctggg tgtggtgtcc caaggccagc caacactggt catcatggaa     3240

ctgatgacac ggggcgatct caaaagttat ctccggtctc tgaggccaga aatggagaat     3300

aatccagtcc tagcacctcc aagcctgagc aagatgattc agatggccgg agagattgca     3360

gacggcatgg catacctcaa cgccaataag ttcgtccaca gagaccttgc tgcccggaat     3420

tgcatggtag ccgaagattt cacagtcaaa atcggagatt ttggtatgac gcgagatatc     3480

tatgagacag actattaccg gaaaggaggg aaagggctgc tgcccgtgcg ctggatgtct     3540

cctgagtccc tcaaggatgg agtcttcacc acttactcgg acgtctggtc cttcggggtc     3600

gtcctctggg agatcgccac actggccgag cagccctacc agggcttgtc caacgagcaa     3660

gtccttcgct tcgtcatgga gggcggcctt ctggacaagc cagacaactg tcctgacatg     3720

ctgtttgaac tgatgcgcat gtgctggcag tataacccca agatgaggcc ttccttcctg     3780

gagatcatca gcagcatcaa agaggagatg gagcctggct tccgggaggt ctccttctac     3840

tacagcgagg agaacaagct gcccgagccg gaggagctgg acctggagcc agagaacatg     3900

gagagcgtcc ccctggaccc ctcggcctcc tcgtcctccc tgccactgcc cgacagacac     3960

tcaggacaca aggccgagaa cggccccggc cctggggtgc tggtcctccg cgccagcttc     4020

gacgagagac agccttacgc ccacatgaac gggggccgca agaacgagcg ggccttgccg     4080

ctgccccagt cttcgacctg cgaattcgtc accgacgcca aaaacataaa gaaaggcccg     4140

gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag     4200

agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc     4260

acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg     4320

ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg     4380

gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa     4440

cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag     4500

gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc     4560

atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat     4620

ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca     4680

attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct     4740

catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc     4800

attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact     4860

acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag     4920

ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta     4980

ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa     5040

attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc     5100

catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt     5160

acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg     5220

aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt     5280

gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg     5340

attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac     5400

ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc     5460

gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt     5520

cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag     5580

acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag     5640

ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac     5700

gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa     5760


<210> 10
<211> 1919
<212> PRT
<213> Artificial Sequence

<220> 
<223> hIGF1R-luc fusion protein

<400> 10
Met Lys Ser Gly Ser Gly Gly Gly Ser Pro Thr Ser Leu Trp Gly Leu 
1               5                   10                  15      
Leu Phe Leu Ser Ala Ala Leu Ser Leu Trp Pro Thr Ser Gly Glu Ile 
            20                  25                  30          
Cys Gly Pro Gly Ile Asp Ile Arg Asn Asp Tyr Gln Gln Leu Lys Arg 
        35                  40                  45              
Leu Glu Asn Cys Thr Val Ile Glu Gly Tyr Leu His Ile Leu Leu Ile 
    50                  55                  60                  
Ser Lys Ala Glu Asp Tyr Arg Ser Tyr Arg Phe Pro Lys Leu Thr Val 
65                  70                  75                  80  
Ile Thr Glu Tyr Leu Leu Leu Phe Arg Val Ala Gly Leu Glu Ser Leu 
                85                  90                  95      
Gly Asp Leu Phe Pro Asn Leu Thr Val Ile Arg Gly Trp Lys Leu Phe 
            100                 105                 110         
Tyr Asn Tyr Ala Leu Val Ile Phe Glu Met Thr Asn Leu Lys Asp Ile 
        115                 120                 125             
Gly Leu Tyr Asn Leu Arg Asn Ile Thr Arg Gly Ala Ile Arg Ile Glu 
    130                 135                 140                 
Lys Asn Ala Asp Leu Cys Tyr Leu Ser Thr Val Asp Trp Ser Leu Ile 
145                 150                 155                 160 
Leu Asp Ala Val Ser Asn Asn Tyr Ile Val Gly Asn Lys Pro Pro Lys 
                165                 170                 175     
Glu Cys Gly Asp Leu Cys Pro Gly Thr Met Glu Glu Lys Pro Met Cys 
            180                 185                 190         
Glu Lys Thr Thr Ile Asn Asn Glu Tyr Asn Tyr Arg Cys Trp Thr Thr 
        195                 200                 205             
Asn Arg Cys Gln Lys Met Cys Pro Ser Thr Cys Gly Lys Arg Ala Cys 
    210                 215                 220                 
Thr Glu Asn Asn Glu Cys Cys His Pro Glu Cys Leu Gly Ser Cys Ser 
225                 230                 235                 240 
Ala Pro Asp Asn Asp Thr Ala Cys Val Ala Cys Arg His Tyr Tyr Tyr 
                245                 250                 255     
Ala Gly Val Cys Val Pro Ala Cys Pro Pro Asn Thr Tyr Arg Phe Glu 
            260                 265                 270         
Gly Trp Arg Cys Val Asp Arg Asp Phe Cys Ala Asn Ile Leu Ser Ala 
        275                 280                 285             
Glu Ser Ser Asp Ser Glu Gly Phe Val Ile His Asp Gly Glu Cys Met 
    290                 295                 300                 
Gln Glu Cys Pro Ser Gly Phe Ile Arg Asn Gly Ser Gln Ser Met Tyr 
305                 310                 315                 320 
Cys Ile Pro Cys Glu Gly Pro Cys Pro Lys Val Cys Glu Glu Glu Lys 
                325                 330                 335     
Lys Thr Lys Thr Ile Asp Ser Val Thr Ser Ala Gln Met Leu Gln Gly 
            340                 345                 350         
Cys Thr Ile Phe Lys Gly Asn Leu Leu Ile Asn Ile Arg Arg Gly Asn 
        355                 360                 365             
Asn Ile Ala Ser Glu Leu Glu Asn Phe Met Gly Leu Ile Glu Val Val 
    370                 375                 380                 
Thr Gly Tyr Val Lys Ile Arg His Ser His Ala Leu Val Ser Leu Ser 
385                 390                 395                 400 
Phe Leu Lys Asn Leu Arg Leu Ile Leu Gly Glu Glu Gln Leu Glu Gly 
                405                 410                 415     
Asn Tyr Ser Phe Tyr Val Leu Asp Asn Gln Asn Leu Gln Gln Leu Trp 
            420                 425                 430         
Asp Trp Asp His Arg Asn Leu Thr Ile Lys Ala Gly Lys Met Tyr Phe 
        435                 440                 445             
Ala Phe Asn Pro Lys Leu Cys Val Ser Glu Ile Tyr Arg Met Glu Glu 
    450                 455                 460                 
Val Thr Gly Thr Lys Gly Arg Gln Ser Lys Gly Asp Ile Asn Thr Arg 
465                 470                 475                 480 
Asn Asn Gly Glu Arg Ala Ser Cys Glu Ser Asp Val Leu His Phe Thr 
                485                 490                 495     
Ser Thr Thr Thr Ser Lys Asn Arg Ile Ile Ile Thr Trp His Arg Tyr 
            500                 505                 510         
Arg Pro Pro Asp Tyr Arg Asp Leu Ile Ser Phe Thr Val Tyr Tyr Lys 
        515                 520                 525             
Glu Ala Pro Phe Lys Asn Val Thr Glu Tyr Asp Gly Gln Asp Ala Cys 
    530                 535                 540                 
Gly Ser Asn Ser Trp Asn Met Val Asp Val Asp Leu Pro Pro Asn Lys 
545                 550                 555                 560 
Asp Val Glu Pro Gly Ile Leu Leu His Gly Leu Lys Pro Trp Thr Gln 
                565                 570                 575     
Tyr Ala Val Tyr Val Lys Ala Val Thr Leu Thr Met Val Glu Asn Asp 
            580                 585                 590         
His Ile Arg Gly Ala Lys Ser Glu Ile Leu Tyr Ile Arg Thr Asn Ala 
        595                 600                 605             
Ser Val Pro Ser Ile Pro Leu Asp Val Leu Ser Ala Ser Asn Ser Ser 
    610                 615                 620                 
Ser Gln Leu Ile Val Lys Trp Asn Pro Pro Ser Leu Pro Asn Gly Asn 
625                 630                 635                 640 
Leu Ser Tyr Tyr Ile Val Arg Trp Gln Arg Gln Pro Gln Asp Gly Tyr 
                645                 650                 655     
Leu Tyr Arg His Asn Tyr Cys Ser Lys Asp Lys Ile Pro Ile Arg Lys 
            660                 665                 670         
Tyr Ala Asp Gly Thr Ile Asp Ile Glu Glu Val Thr Glu Asn Pro Lys 
        675                 680                 685             
Thr Glu Val Cys Gly Gly Glu Lys Gly Pro Cys Cys Ala Cys Pro Lys 
    690                 695                 700                 
Thr Glu Ala Glu Lys Gln Ala Glu Lys Glu Glu Ala Glu Tyr Arg Lys 
705                 710                 715                 720 
Val Phe Glu Asn Phe Leu His Asn Ser Ile Phe Val Pro Arg Pro Glu 
                725                 730                 735     
Arg Lys Arg Arg Asp Val Met Gln Val Ala Asn Thr Thr Met Ser Ser 
            740                 745                 750         
Arg Ser Arg Asn Thr Thr Ala Ala Asp Thr Tyr Asn Ile Thr Asp Pro 
        755                 760                 765             
Glu Glu Leu Glu Thr Glu Tyr Pro Phe Phe Glu Ser Arg Val Asp Asn 
    770                 775                 780                 
Lys Glu Arg Thr Val Ile Ser Asn Leu Arg Pro Phe Thr Leu Tyr Arg 
785                 790                 795                 800 
Ile Asp Ile His Ser Cys Asn His Glu Ala Glu Lys Leu Gly Cys Ser 
                805                 810                 815     
Ala Ser Asn Phe Val Phe Ala Arg Thr Met Pro Ala Glu Gly Ala Asp 
            820                 825                 830         
Asp Ile Pro Gly Pro Val Thr Trp Glu Pro Arg Pro Glu Asn Ser Ile 
        835                 840                 845             
Phe Leu Lys Trp Pro Glu Pro Glu Asn Pro Asn Gly Leu Ile Leu Met 
    850                 855                 860                 
Tyr Glu Ile Lys Tyr Gly Ser Gln Val Glu Asp Gln Arg Glu Cys Val 
865                 870                 875                 880 
Ser Arg Gln Glu Tyr Arg Lys Tyr Gly Gly Ala Lys Leu Asn Arg Leu 
                885                 890                 895     
Asn Pro Gly Asn Tyr Thr Ala Arg Ile Gln Ala Thr Ser Leu Ser Gly 
            900                 905                 910         
Asn Gly Ser Trp Thr Asp Pro Val Phe Phe Tyr Val Gln Ala Lys Thr 
        915                 920                 925             
Gly Tyr Glu Asn Phe Ile His Leu Ile Ile Ala Leu Pro Val Ala Val 
    930                 935                 940                 
Leu Leu Ile Val Gly Gly Leu Val Ile Met Leu Tyr Val Phe His Arg 
945                 950                 955                 960 
Lys Arg Asn Asn Ser Arg Leu Gly Asn Gly Val Leu Tyr Ala Ser Val 
                965                 970                 975     
Asn Pro Glu Tyr Phe Ser Ala Ala Asp Val Tyr Val Pro Asp Glu Trp 
            980                 985                 990         
Glu Val Ala Arg Glu Lys Ile Thr Met Ser Arg Glu Leu Gly Gln Gly 
        995                 1000                1005            
Ser Phe Gly Met Val Tyr Glu Gly Val Ala Lys Gly Val Val Lys Asp 
    1010                1015                1020                
Glu Pro Glu Thr Arg Val Ala Ile Lys Thr Val Asn Glu Ala Ala Ser 
1025                1030                1035                1040
Met Arg Glu Arg Ile Glu Phe Leu Asn Glu Ala Ser Val Met Lys Glu 
                1045                1050                1055    
Phe Asn Cys His His Val Val Arg Leu Leu Gly Val Val Ser Gln Gly 
            1060                1065                1070        
Gln Pro Thr Leu Val Ile Met Glu Leu Met Thr Arg Gly Asp Leu Lys 
        1075                1080                1085            
Ser Tyr Leu Arg Ser Leu Arg Pro Glu Met Glu Asn Asn Pro Val Leu 
    1090                1095                1100                
Ala Pro Pro Ser Leu Ser Lys Met Ile Gln Met Ala Gly Glu Ile Ala 
1105                1110                1115                1120
Asp Gly Met Ala Tyr Leu Asn Ala Asn Lys Phe Val His Arg Asp Leu 
                1125                1130                1135    
Ala Ala Arg Asn Cys Met Val Ala Glu Asp Phe Thr Val Lys Ile Gly 
            1140                1145                1150        
Asp Phe Gly Met Thr Arg Asp Ile Tyr Glu Thr Asp Tyr Tyr Arg Lys 
        1155                1160                1165            
Gly Gly Lys Gly Leu Leu Pro Val Arg Trp Met Ser Pro Glu Ser Leu 
    1170                1175                1180                
Lys Asp Gly Val Phe Thr Thr Tyr Ser Asp Val Trp Ser Phe Gly Val 
1185                1190                1195                1200
Val Leu Trp Glu Ile Ala Thr Leu Ala Glu Gln Pro Tyr Gln Gly Leu 
                1205                1210                1215    
Ser Asn Glu Gln Val Leu Arg Phe Val Met Glu Gly Gly Leu Leu Asp 
            1220                1225                1230        
Lys Pro Asp Asn Cys Pro Asp Met Leu Phe Glu Leu Met Arg Met Cys 
        1235                1240                1245            
Trp Gln Tyr Asn Pro Lys Met Arg Pro Ser Phe Leu Glu Ile Ile Ser 
    1250                1255                1260                
Ser Ile Lys Glu Glu Met Glu Pro Gly Phe Arg Glu Val Ser Phe Tyr 
1265                1270                1275                1280
Tyr Ser Glu Glu Asn Lys Leu Pro Glu Pro Glu Glu Leu Asp Leu Glu 
                1285                1290                1295    
Pro Glu Asn Met Glu Ser Val Pro Leu Asp Pro Ser Ala Ser Ser Ser 
            1300                1305                1310        
Ser Leu Pro Leu Pro Asp Arg His Ser Gly His Lys Ala Glu Asn Gly 
        1315                1320                1325            
Pro Gly Pro Gly Val Leu Val Leu Arg Ala Ser Phe Asp Glu Arg Gln 
    1330                1335                1340                
Pro Tyr Ala His Met Asn Gly Gly Arg Lys Asn Glu Arg Ala Leu Pro 
1345                1350                1355                1360
Leu Pro Gln Ser Ser Thr Cys Glu Phe Val Thr Asp Ala Lys Asn Ile 
                1365                1370                1375    
Lys Lys Gly Pro Ala Pro Phe Tyr Pro Leu Glu Asp Gly Thr Ala Gly 
            1380                1385                1390        
Glu Gln Leu His Lys Ala Met Lys Arg Tyr Ala Leu Val Pro Gly Thr 
        1395                1400                1405            
Ile Ala Phe Thr Asp Ala His Ile Glu Val Asp Ile Thr Tyr Ala Glu 
    1410                1415                1420                
Tyr Phe Glu Met Ser Val Arg Leu Ala Glu Ala Met Lys Arg Tyr Gly 
1425                1430                1435                1440
Leu Asn Thr Asn His Arg Ile Val Val Cys Ser Glu Asn Ser Leu Gln 
                1445                1450                1455    
Phe Phe Met Pro Val Leu Gly Ala Leu Phe Ile Gly Val Ala Val Ala 
            1460                1465                1470        
Pro Ala Asn Asp Ile Tyr Asn Glu Arg Glu Leu Leu Asn Ser Met Gly 
        1475                1480                1485            
Ile Ser Gln Pro Thr Val Val Phe Val Ser Lys Lys Gly Leu Gln Lys 
    1490                1495                1500                
Ile Leu Asn Val Gln Lys Lys Leu Pro Ile Ile Gln Lys Ile Ile Ile 
1505                1510                1515                1520
Met Asp Ser Lys Thr Asp Tyr Gln Gly Phe Gln Ser Met Tyr Thr Phe 
                1525                1530                1535    
Val Thr Ser His Leu Pro Pro Gly Phe Asn Glu Tyr Asp Phe Val Pro 
            1540                1545                1550        
Glu Ser Phe Asp Arg Asp Lys Thr Ile Ala Leu Ile Met Asn Ser Ser 
        1555                1560                1565            
Gly Ser Thr Gly Leu Pro Lys Gly Val Ala Leu Pro His Arg Thr Ala 
    1570                1575                1580                
Cys Val Arg Phe Ser His Ala Arg Asp Pro Ile Phe Gly Asn Gln Ile 
1585                1590                1595                1600
Ile Pro Asp Thr Ala Ile Leu Ser Val Val Pro Phe His His Gly Phe 
                1605                1610                1615    
Gly Met Phe Thr Thr Leu Gly Tyr Leu Ile Cys Gly Phe Arg Val Val 
            1620                1625                1630        
Leu Met Tyr Arg Phe Glu Glu Glu Leu Phe Leu Arg Ser Leu Gln Asp 
        1635                1640                1645            
Tyr Lys Ile Gln Ser Ala Leu Leu Val Pro Thr Leu Phe Ser Phe Phe 
    1650                1655                1660                
Ala Lys Ser Thr Leu Ile Asp Lys Tyr Asp Leu Ser Asn Leu His Glu 
1665                1670                1675                1680
Ile Ala Ser Gly Gly Ala Pro Leu Ser Lys Glu Val Gly Glu Ala Val 
                1685                1690                1695    
Ala Lys Arg Phe His Leu Pro Gly Ile Arg Gln Gly Tyr Gly Leu Thr 
            1700                1705                1710        
Glu Thr Thr Ser Ala Ile Leu Ile Thr Pro Glu Gly Asp Asp Lys Pro 
        1715                1720                1725            
Gly Ala Val Gly Lys Val Val Pro Phe Phe Glu Ala Lys Val Val Asp 
    1730                1735                1740                
Leu Asp Thr Gly Lys Thr Leu Gly Val Asn Gln Arg Gly Glu Leu Cys 
1745                1750                1755                1760
Val Arg Gly Pro Met Ile Met Ser Gly Tyr Val Asn Asn Pro Glu Ala 
                1765                1770                1775    
Thr Asn Ala Leu Ile Asp Lys Asp Gly Trp Leu His Ser Gly Asp Ile 
            1780                1785                1790        
Ala Tyr Trp Asp Glu Asp Glu His Phe Phe Ile Val Asp Arg Leu Lys 
        1795                1800                1805            
Ser Leu Ile Lys Tyr Lys Gly Tyr Gln Val Ala Pro Ala Glu Leu Glu 
    1810                1815                1820                
Ser Ile Leu Leu Gln His Pro Asn Ile Phe Asp Ala Gly Val Ala Gly 
1825                1830                1835                1840
Leu Pro Asp Asp Asp Ala Gly Glu Leu Pro Ala Ala Val Val Val Leu 
                1845                1850                1855    
Glu His Gly Lys Thr Met Thr Glu Lys Glu Ile Val Asp Tyr Val Ala 
            1860                1865                1870        
Ser Gln Val Thr Thr Ala Lys Lys Leu Arg Gly Gly Val Val Phe Val 
        1875                1880                1885            
Asp Glu Val Pro Lys Gly Leu Thr Gly Lys Leu Asp Ala Arg Lys Ile 
    1890                1895                1900                
Arg Glu Ile Leu Ile Lys Ala Lys Lys Gly Gly Lys Ile Ala Val 
1905                1910                1915                

<210> 11
<211> 4113
<212> DNA
<213> Homo sapiens

<220> 
<221> source
<222> 1..4113
<223> /organism="Homo sapiens"
      /note="cDNA insulin receptor"
      /mol_type="unassigned DNA"

<400> 11
atgggcaccg ggggccggcg gggggcggcg gccgcgccgc tgctggtggc ggtggccgcg       60

ctgctactgg gcgccgcggg ccacctgtac cccggagagg tgtgtcccgg catggatatc      120

cggaacaacc tcactaggtt gcatgagctg gagaattgct ctgtcatcga aggacacttg      180

cagatactct tgatgttcaa aacgaggccc gaagatttcc gagacctcag tttccccaaa      240

ctcatcatga tcactgatta cttgctgctc ttccgggtct atgggctcga gagcctgaag      300

gacctgttcc ccaacctcac ggtcatccgg ggatcacgac tgttctttaa ctacgcgctg      360

gtcatcttcg agatggttca cctcaaggaa ctcggcctct acaacctgat gaacatcacc      420

cggggttctg tccgcatcga gaagaacaat gagctctgtt acttggccac tatcgactgg      480

tcccgtatcc tggattccgt ggaggataat tacatcgtgt tgaacaaaga tgacaacgag      540

gagtgtggag acatctgtcc gggtaccgcg aagggcaaga ccaactgccc cgccaccgtc      600

atcaacgggc agtttgtcga acgatgttgg actcatagtc actgccagaa agtttgcccg      660

accatctgta agtcacacgg ctgcaccgcc gaaggcctct gttgccacag cgagtgcctg      720

ggcaactgtt ctcagcccga cgaccccacc aagtgcgtgg cctgccgcaa cttctacctg      780

gacggcaggt gtgtggagac ctgcccgccc ccgtactacc acttccagga ctggcgctgt      840

gtgaacttca gcttctgcca ggacctgcac cacaaatgca agaactcgcg gaggcagggc      900

tgccaccagt acgtcattca caacaacaag tgcatccctg agtgtccctc cgggtacacg      960

atgaattcca gcaacttgct gtgcacccca tgcctgggtc cctgtcccaa ggtgtgccac     1020

ctcctagaag gcgagaagac catcgactcg gtgacgtctg cccaggagct ccgaggatgc     1080

accgtcatca acgggagtct gatcatcaac attcgaggag gcaacaatct ggcagctgag     1140

ctagaagcca acctcggcct cattgaagaa atttcagggt atctaaaaat ccgccgatcc     1200

tacgctctgg tgtcactttc cttcttccgg aagttacgtc tgattcgagg agagaccttg     1260

gaaattggga actactcctt ctatgccttg gacaaccaga acctaaggca gctctgggac     1320

tggagcaaac acaacctcac catcactcag gggaaactct tcttccacta taaccccaaa     1380

ctctgcttgt cagaaatcca caagatggaa gaagtttcag gaaccaaggg gcgccaggag     1440

agaaacgaca ttgccctgaa gaccaatggg gaccaggcat cctgtgaaaa tgagttactt     1500

aaattttctt acattcggac atcttttgac aagatcttgc tgagatggga gccgtactgg     1560

ccccccgact tccgagacct cttggggttc atgctgttct acaaagaggc cccttatcag     1620

aatgtgacgg agttcgacgg gcaggatgcg tgtggttcca acagttggac ggtggtagac     1680

attgacccac ccctgaggtc caacgacccc aaatcacaga accacccagg gtggctgatg     1740

cggggtctca agccctggac ccagtatgcc atctttgtga agaccctggt caccttttcg     1800

gatgaacgcc ggacctatgg ggccaagagt gacatcattt atgtccagac agatgccacc     1860

aacccctctg tgcccctgga tccaatctca gtgtctaact catcatccca gattattctg     1920

aagtggaaac caccctccga ccccaatggc aacatcaccc actacctggt tttctgggag     1980

aggcaggcgg aagacagtga gctgttcgag ctggattatt gcctcaaagg gctgaagctg     2040

ccctcgagga cctggtctcc accattcgag tctgaagatt ctcagaagca caaccagagt     2100

gagtatgagg attcggccgg cgaatgctgc tcctgtccaa agacagactc tcagatcctg     2160

aaggagctgg aggagtcctc gtttaggaag acgtttgagg attacctgca caacgtggtt     2220

ttcgtcccca ggccatctcg gaaacgcagg tcccttggcg atgttgggaa tgtgacggtg     2280

gccgtgccca cggtggcagc tttccccaac acttcctcga ccagcgtgcc cacgagtccg     2340

gaggagcaca ggccttttga gaaggtggtg aacaaggagt cgctggtcat ctccggcttg     2400

cgacacttca cgggctatcg catcgagctg caggcttgca accaggacac ccctgaggaa     2460

cggtgcagtg tggcagccta cgtcagtgcg aggaccatgc ctgaagccaa ggctgatgac     2520

attgttggcc ctgtgacgca tgaaatcttt gagaacaacg tcgtccactt gatgtggcag     2580

gagccgaagg agcccaatgg tctgatcgtg ctgtatgaag tgagttatcg gcgatatggt     2640

gatgaggagc tgcatctctg cgtctcccgc aagcacttcg ctctggaacg gggctgcagg     2700

ctgcgtgggc tgtcaccggg gaactacagc gtgcgaatcc gggccacctc ccttgcgggc     2760

aacggctctt ggacggaacc cacctatttc tacgtgacag actatttaga cgtcccgtca     2820

aatattgcaa aaattatcat cggccccctc atctttgtct ttctcttcag tgttgtgatt     2880

ggaagtattt atctattcct gagaaagagg cagccagatg ggccgctggg accgctttac     2940

gcttcttcaa accctgagta tctcagtgcc agtgatgtgt ttccatgctc tgtgtacgtg     3000

ccggacgagt gggaggtgtc tcgagagaag atcaccctcc ttcgagagct ggggcagggc     3060

tccttcggca tggtgtatga gggcaatgcc agggacatca tcaagggtga ggcagagacc     3120

cgcgtggcgg tgaagacggt caacgagtca gccagtctcc gagagcggat tgagttcctc     3180

aatgaggcct cggtcatgaa gggcttcacc tgccatcatg tggtgcgcct cctgggagtg     3240

gtgtccaagg gccagcccac gctggtggtg atggagctga tggctcacgg agacctgaag     3300

agctacctcc gttctctgcg gccagaggct gagaataatc ctggccgccc tccccctacc     3360

cttcaagaga tgattcagat ggcggcagag attgctgacg ggatggccta cctgaacgcc     3420

aagaagtttg tgcatcggga cctggcagcg agaaactgca tggtcgccca tgattttact     3480

gtcaaaattg gagactttgg aatgaccaga gacatctatg aaacggatta ctaccggaaa     3540

gggggcaagg gtctgctccc tgtacggtgg atggcaccgg agtccctgaa ggatggggtc     3600

ttcaccactt cttctgacat gtggtccttt ggcgtggtcc tttgggaaat caccagcttg     3660

gcagaacagc cttaccaagg cctgtctaat gaacaggtgt tgaaatttgt catggatgga     3720

gggtatctgg atcaacccga caactgtcca gagagagtca ctgacctcat gcgcatgtgc     3780

tggcaattca accccaagat gaggccaacc ttcctggaga ttgtcaacct gctcaaggac     3840

gacctgcacc ccagctttcc agaggtgtcg ttcttccaca gcgaggagaa caaggctccc     3900

gagagtgagg agctggagat ggagtttgag gacatggaga atgtgcccct ggaccgttcc     3960

tcgcactgtc agagggagga ggcggggggc cgggatggag ggtcctcgct gggtttcaag     4020

cggagctacg aggaacacat cccttacaca cacatgaacg gaggcaagaa aaacgggcgg     4080

attctgacct tgcctcggtc caatccttcc taa                                  4113


<210> 12
<211> 1370
<212> PRT
<213> Homo sapiens

<220> 
<223> insulin receptor

<400> 12
Met Gly Thr Gly Gly Arg Arg Gly Ala Ala Ala Ala Pro Leu Leu Val 
1               5                   10                  15      
Ala Val Ala Ala Leu Leu Leu Gly Ala Ala Gly His Leu Tyr Pro Gly 
            20                  25                  30          
Glu Val Cys Pro Gly Met Asp Ile Arg Asn Asn Leu Thr Arg Leu His 
        35                  40                  45              
Glu Leu Glu Asn Cys Ser Val Ile Glu Gly His Leu Gln Ile Leu Leu 
    50                  55                  60                  
Met Phe Lys Thr Arg Pro Glu Asp Phe Arg Asp Leu Ser Phe Pro Lys 
65                  70                  75                  80  
Leu Ile Met Ile Thr Asp Tyr Leu Leu Leu Phe Arg Val Tyr Gly Leu 
                85                  90                  95      
Glu Ser Leu Lys Asp Leu Phe Pro Asn Leu Thr Val Ile Arg Gly Ser 
            100                 105                 110         
Arg Leu Phe Phe Asn Tyr Ala Leu Val Ile Phe Glu Met Val His Leu 
        115                 120                 125             
Lys Glu Leu Gly Leu Tyr Asn Leu Met Asn Ile Thr Arg Gly Ser Val 
    130                 135                 140                 
Arg Ile Glu Lys Asn Asn Glu Leu Cys Tyr Leu Ala Thr Ile Asp Trp 
145                 150                 155                 160 
Ser Arg Ile Leu Asp Ser Val Glu Asp Asn Tyr Ile Val Leu Asn Lys 
                165                 170                 175     
Asp Asp Asn Glu Glu Cys Gly Asp Ile Cys Pro Gly Thr Ala Lys Gly 
            180                 185                 190         
Lys Thr Asn Cys Pro Ala Thr Val Ile Asn Gly Gln Phe Val Glu Arg 
        195                 200                 205             
Cys Trp Thr His Ser His Cys Gln Lys Val Cys Pro Thr Ile Cys Lys 
    210                 215                 220                 
Ser His Gly Cys Thr Ala Glu Gly Leu Cys Cys His Ser Glu Cys Leu 
225                 230                 235                 240 
Gly Asn Cys Ser Gln Pro Asp Asp Pro Thr Lys Cys Val Ala Cys Arg 
                245                 250                 255     
Asn Phe Tyr Leu Asp Gly Arg Cys Val Glu Thr Cys Pro Pro Pro Tyr 
            260                 265                 270         
Tyr His Phe Gln Asp Trp Arg Cys Val Asn Phe Ser Phe Cys Gln Asp 
        275                 280                 285             
Leu His His Lys Cys Lys Asn Ser Arg Arg Gln Gly Cys His Gln Tyr 
    290                 295                 300                 
Val Ile His Asn Asn Lys Cys Ile Pro Glu Cys Pro Ser Gly Tyr Thr 
305                 310                 315                 320 
Met Asn Ser Ser Asn Leu Leu Cys Thr Pro Cys Leu Gly Pro Cys Pro 
                325                 330                 335     
Lys Val Cys His Leu Leu Glu Gly Glu Lys Thr Ile Asp Ser Val Thr 
            340                 345                 350         
Ser Ala Gln Glu Leu Arg Gly Cys Thr Val Ile Asn Gly Ser Leu Ile 
        355                 360                 365             
Ile Asn Ile Arg Gly Gly Asn Asn Leu Ala Ala Glu Leu Glu Ala Asn 
    370                 375                 380                 
Leu Gly Leu Ile Glu Glu Ile Ser Gly Tyr Leu Lys Ile Arg Arg Ser 
385                 390                 395                 400 
Tyr Ala Leu Val Ser Leu Ser Phe Phe Arg Lys Leu Arg Leu Ile Arg 
                405                 410                 415     
Gly Glu Thr Leu Glu Ile Gly Asn Tyr Ser Phe Tyr Ala Leu Asp Asn 
            420                 425                 430         
Gln Asn Leu Arg Gln Leu Trp Asp Trp Ser Lys His Asn Leu Thr Ile 
        435                 440                 445             
Thr Gln Gly Lys Leu Phe Phe His Tyr Asn Pro Lys Leu Cys Leu Ser 
    450                 455                 460                 
Glu Ile His Lys Met Glu Glu Val Ser Gly Thr Lys Gly Arg Gln Glu 
465                 470                 475                 480 
Arg Asn Asp Ile Ala Leu Lys Thr Asn Gly Asp Gln Ala Ser Cys Glu 
                485                 490                 495     
Asn Glu Leu Leu Lys Phe Ser Tyr Ile Arg Thr Ser Phe Asp Lys Ile 
            500                 505                 510         
Leu Leu Arg Trp Glu Pro Tyr Trp Pro Pro Asp Phe Arg Asp Leu Leu 
        515                 520                 525             
Gly Phe Met Leu Phe Tyr Lys Glu Ala Pro Tyr Gln Asn Val Thr Glu 
    530                 535                 540                 
Phe Asp Gly Gln Asp Ala Cys Gly Ser Asn Ser Trp Thr Val Val Asp 
545                 550                 555                 560 
Ile Asp Pro Pro Leu Arg Ser Asn Asp Pro Lys Ser Gln Asn His Pro 
                565                 570                 575     
Gly Trp Leu Met Arg Gly Leu Lys Pro Trp Thr Gln Tyr Ala Ile Phe 
            580                 585                 590         
Val Lys Thr Leu Val Thr Phe Ser Asp Glu Arg Arg Thr Tyr Gly Ala 
        595                 600                 605             
Lys Ser Asp Ile Ile Tyr Val Gln Thr Asp Ala Thr Asn Pro Ser Val 
    610                 615                 620                 
Pro Leu Asp Pro Ile Ser Val Ser Asn Ser Ser Ser Gln Ile Ile Leu 
625                 630                 635                 640 
Lys Trp Lys Pro Pro Ser Asp Pro Asn Gly Asn Ile Thr His Tyr Leu 
                645                 650                 655     
Val Phe Trp Glu Arg Gln Ala Glu Asp Ser Glu Leu Phe Glu Leu Asp 
            660                 665                 670         
Tyr Cys Leu Lys Gly Leu Lys Leu Pro Ser Arg Thr Trp Ser Pro Pro 
        675                 680                 685             
Phe Glu Ser Glu Asp Ser Gln Lys His Asn Gln Ser Glu Tyr Glu Asp 
    690                 695                 700                 
Ser Ala Gly Glu Cys Cys Ser Cys Pro Lys Thr Asp Ser Gln Ile Leu 
705                 710                 715                 720 
Lys Glu Leu Glu Glu Ser Ser Phe Arg Lys Thr Phe Glu Asp Tyr Leu 
                725                 730                 735     
His Asn Val Val Phe Val Pro Arg Pro Ser Arg Lys Arg Arg Ser Leu 
            740                 745                 750         
Gly Asp Val Gly Asn Val Thr Val Ala Val Pro Thr Val Ala Ala Phe 
        755                 760                 765             
Pro Asn Thr Ser Ser Thr Ser Val Pro Thr Ser Pro Glu Glu His Arg 
    770                 775                 780                 
Pro Phe Glu Lys Val Val Asn Lys Glu Ser Leu Val Ile Ser Gly Leu 
785                 790                 795                 800 
Arg His Phe Thr Gly Tyr Arg Ile Glu Leu Gln Ala Cys Asn Gln Asp 
                805                 810                 815     
Thr Pro Glu Glu Arg Cys Ser Val Ala Ala Tyr Val Ser Ala Arg Thr 
            820                 825                 830         
Met Pro Glu Ala Lys Ala Asp Asp Ile Val Gly Pro Val Thr His Glu 
        835                 840                 845             
Ile Phe Glu Asn Asn Val Val His Leu Met Trp Gln Glu Pro Lys Glu 
    850                 855                 860                 
Pro Asn Gly Leu Ile Val Leu Tyr Glu Val Ser Tyr Arg Arg Tyr Gly 
865                 870                 875                 880 
Asp Glu Glu Leu His Leu Cys Val Ser Arg Lys His Phe Ala Leu Glu 
                885                 890                 895     
Arg Gly Cys Arg Leu Arg Gly Leu Ser Pro Gly Asn Tyr Ser Val Arg 
            900                 905                 910         
Ile Arg Ala Thr Ser Leu Ala Gly Asn Gly Ser Trp Thr Glu Pro Thr 
        915                 920                 925             
Tyr Phe Tyr Val Thr Asp Tyr Leu Asp Val Pro Ser Asn Ile Ala Lys 
    930                 935                 940                 
Ile Ile Ile Gly Pro Leu Ile Phe Val Phe Leu Phe Ser Val Val Ile 
945                 950                 955                 960 
Gly Ser Ile Tyr Leu Phe Leu Arg Lys Arg Gln Pro Asp Gly Pro Leu 
                965                 970                 975     
Gly Pro Leu Tyr Ala Ser Ser Asn Pro Glu Tyr Leu Ser Ala Ser Asp 
            980                 985                 990         
Val Phe Pro Cys Ser Val Tyr Val Pro Asp Glu Trp Glu Val Ser Arg 
        995                 1000                1005            
Glu Lys Ile Thr Leu Leu Arg Glu Leu Gly Gln Gly Ser Phe Gly Met 
    1010                1015                1020                
Val Tyr Glu Gly Asn Ala Arg Asp Ile Ile Lys Gly Glu Ala Glu Thr 
1025                1030                1035                1040
Arg Val Ala Val Lys Thr Val Asn Glu Ser Ala Ser Leu Arg Glu Arg 
                1045                1050                1055    
Ile Glu Phe Leu Asn Glu Ala Ser Val Met Lys Gly Phe Thr Cys His 
            1060                1065                1070        
His Val Val Arg Leu Leu Gly Val Val Ser Lys Gly Gln Pro Thr Leu 
        1075                1080                1085            
Val Val Met Glu Leu Met Ala His Gly Asp Leu Lys Ser Tyr Leu Arg 
    1090                1095                1100                
Ser Leu Arg Pro Glu Ala Glu Asn Asn Pro Gly Arg Pro Pro Pro Thr 
1105                1110                1115                1120
Leu Gln Glu Met Ile Gln Met Ala Ala Glu Ile Ala Asp Gly Met Ala 
                1125                1130                1135    
Tyr Leu Asn Ala Lys Lys Phe Val His Arg Asp Leu Ala Ala Arg Asn 
            1140                1145                1150        
Cys Met Val Ala His Asp Phe Thr Val Lys Ile Gly Asp Phe Gly Met 
        1155                1160                1165            
Thr Arg Asp Ile Tyr Glu Thr Asp Tyr Tyr Arg Lys Gly Gly Lys Gly 
    1170                1175                1180                
Leu Leu Pro Val Arg Trp Met Ala Pro Glu Ser Leu Lys Asp Gly Val 
1185                1190                1195                1200
Phe Thr Thr Ser Ser Asp Met Trp Ser Phe Gly Val Val Leu Trp Glu 
                1205                1210                1215    
Ile Thr Ser Leu Ala Glu Gln Pro Tyr Gln Gly Leu Ser Asn Glu Gln 
            1220                1225                1230        
Val Leu Lys Phe Val Met Asp Gly Gly Tyr Leu Asp Gln Pro Asp Asn 
        1235                1240                1245            
Cys Pro Glu Arg Val Thr Asp Leu Met Arg Met Cys Trp Gln Phe Asn 
    1250                1255                1260                
Pro Lys Met Arg Pro Thr Phe Leu Glu Ile Val Asn Leu Leu Lys Asp 
1265                1270                1275                1280
Asp Leu His Pro Ser Phe Pro Glu Val Ser Phe Phe His Ser Glu Glu 
                1285                1290                1295    
Asn Lys Ala Pro Glu Ser Glu Glu Leu Glu Met Glu Phe Glu Asp Met 
            1300                1305                1310        
Glu Asn Val Pro Leu Asp Arg Ser Ser His Cys Gln Arg Glu Glu Ala 
        1315                1320                1325            
Gly Gly Arg Asp Gly Gly Ser Ser Leu Gly Phe Lys Arg Ser Tyr Glu 
    1330                1335                1340                
Glu His Ile Pro Tyr Thr His Met Asn Gly Gly Lys Lys Asn Gly Arg 
1345                1350                1355                1360
Ile Leu Thr Leu Pro Arg Ser Asn Pro Ser 
                1365                1370

<210> 13
<211> 5769
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..5769
<223> /organism="Artificial Sequence"
      /note="human insulin receptor-luc fusion"
      /mol_type="unassigned DNA"

<400> 13
atgggcaccg ggggccggcg gggggcggcg gccgcgccgc tgctggtggc ggtggccgcg       60

ctgctactgg gcgccgcggg ccacctgtac cccggagagg tgtgtcccgg catggatatc      120

cggaacaacc tcactaggtt gcatgagctg gagaattgct ctgtcatcga aggacacttg      180

cagatactct tgatgttcaa aacgaggccc gaagatttcc gagacctcag tttccccaaa      240

ctcatcatga tcactgatta cttgctgctc ttccgggtct atgggctcga gagcctgaag      300

gacctgttcc ccaacctcac ggtcatccgg ggatcacgac tgttctttaa ctacgcgctg      360

gtcatcttcg agatggttca cctcaaggaa ctcggcctct acaacctgat gaacatcacc      420

cggggttctg tccgcatcga gaagaacaat gagctctgtt acttggccac tatcgactgg      480

tcccgtatcc tggattccgt ggaggataat tacatcgtgt tgaacaaaga tgacaacgag      540

gagtgtggag acatctgtcc gggtaccgcg aagggcaaga ccaactgccc cgccaccgtc      600

atcaacgggc agtttgtcga acgatgttgg actcatagtc actgccagaa agtttgcccg      660

accatctgta agtcacacgg ctgcaccgcc gaaggcctct gttgccacag cgagtgcctg      720

ggcaactgtt ctcagcccga cgaccccacc aagtgcgtgg cctgccgcaa cttctacctg      780

gacggcaggt gtgtggagac ctgcccgccc ccgtactacc acttccagga ctggcgctgt      840

gtgaacttca gcttctgcca ggacctgcac cacaaatgca agaactcgcg gaggcagggc      900

tgccaccagt acgtcattca caacaacaag tgcatccctg agtgtccctc cgggtacacg      960

atgaattcca gcaacttgct gtgcacccca tgcctgggtc cctgtcccaa ggtgtgccac     1020

ctcctagaag gcgagaagac catcgactcg gtgacgtctg cccaggagct ccgaggatgc     1080

accgtcatca acgggagtct gatcatcaac attcgaggag gcaacaatct ggcagctgag     1140

ctagaagcca acctcggcct cattgaagaa atttcagggt atctaaaaat ccgccgatcc     1200

tacgctctgg tgtcactttc cttcttccgg aagttacgtc tgattcgagg agagaccttg     1260

gaaattggga actactcctt ctatgccttg gacaaccaga acctaaggca gctctgggac     1320

tggagcaaac acaacctcac catcactcag gggaaactct tcttccacta taaccccaaa     1380

ctctgcttgt cagaaatcca caagatggaa gaagtttcag gaaccaaggg gcgccaggag     1440

agaaacgaca ttgccctgaa gaccaatggg gaccaggcat cctgtgaaaa tgagttactt     1500

aaattttctt acattcggac atcttttgac aagatcttgc tgagatggga gccgtactgg     1560

ccccccgact tccgagacct cttggggttc atgctgttct acaaagaggc cccttatcag     1620

aatgtgacgg agttcgacgg gcaggatgcg tgtggttcca acagttggac ggtggtagac     1680

attgacccac ccctgaggtc caacgacccc aaatcacaga accacccagg gtggctgatg     1740

cggggtctca agccctggac ccagtatgcc atctttgtga agaccctggt caccttttcg     1800

gatgaacgcc ggacctatgg ggccaagagt gacatcattt atgtccagac agatgccacc     1860

aacccctctg tgcccctgga tccaatctca gtgtctaact catcatccca gattattctg     1920

aagtggaaac caccctccga ccccaatggc aacatcaccc actacctggt tttctgggag     1980

aggcaggcgg aagacagtga gctgttcgag ctggattatt gcctcaaagg gctgaagctg     2040

ccctcgagga cctggtctcc accattcgag tctgaagatt ctcagaagca caaccagagt     2100

gagtatgagg attcggccgg cgaatgctgc tcctgtccaa agacagactc tcagatcctg     2160

aaggagctgg aggagtcctc gtttaggaag acgtttgagg attacctgca caacgtggtt     2220

ttcgtcccca ggccatctcg gaaacgcagg tcccttggcg atgttgggaa tgtgacggtg     2280

gccgtgccca cggtggcagc tttccccaac acttcctcga ccagcgtgcc cacgagtccg     2340

gaggagcaca ggccttttga gaaggtggtg aacaaggagt cgctggtcat ctccggcttg     2400

cgacacttca cgggctatcg catcgagctg caggcttgca accaggacac ccctgaggaa     2460

cggtgcagtg tggcagccta cgtcagtgcg aggaccatgc ctgaagccaa ggctgatgac     2520

attgttggcc ctgtgacgca tgaaatcttt gagaacaacg tcgtccactt gatgtggcag     2580

gagccgaagg agcccaatgg tctgatcgtg ctgtatgaag tgagttatcg gcgatatggt     2640

gatgaggagc tgcatctctg cgtctcccgc aagcacttcg ctctggaacg gggctgcagg     2700

ctgcgtgggc tgtcaccggg gaactacagc gtgcgaatcc gggccacctc ccttgcgggc     2760

aacggctctt ggacggaacc cacctatttc tacgtgacag actatttaga cgtcccgtca     2820

aatattgcaa aaattatcat cggccccctc atctttgtct ttctcttcag tgttgtgatt     2880

ggaagtattt atctattcct gagaaagagg cagccagatg ggccgctggg accgctttac     2940

gcttcttcaa accctgagta tctcagtgcc agtgatgtgt ttccatgctc tgtgtacgtg     3000

ccggacgagt gggaggtgtc tcgagagaag atcaccctcc ttcgagagct ggggcagggc     3060

tccttcggca tggtgtatga gggcaatgcc agggacatca tcaagggtga ggcagagacc     3120

cgcgtggcgg tgaagacggt caacgagtca gccagtctcc gagagcggat tgagttcctc     3180

aatgaggcct cggtcatgaa gggcttcacc tgccatcatg tggtgcgcct cctgggagtg     3240

gtgtccaagg gccagcccac gctggtggtg atggagctga tggctcacgg agacctgaag     3300

agctacctcc gttctctgcg gccagaggct gagaataatc ctggccgccc tccccctacc     3360

cttcaagaga tgattcagat ggcggcagag attgctgacg ggatggccta cctgaacgcc     3420

aagaagtttg tgcatcggga cctggcagcg agaaactgca tggtcgccca tgattttact     3480

gtcaaaattg gagactttgg aatgaccaga gacatctatg aaacggatta ctaccggaaa     3540

gggggcaagg gtctgctccc tgtacggtgg atggcaccgg agtccctgaa ggatggggtc     3600

ttcaccactt cttctgacat gtggtccttt ggcgtggtcc tttgggaaat caccagcttg     3660

gcagaacagc cttaccaagg cctgtctaat gaacaggtgt tgaaatttgt catggatgga     3720

gggtatctgg atcaacccga caactgtcca gagagagtca ctgacctcat gcgcatgtgc     3780

tggcaattca accccaagat gaggccaacc ttcctggaga ttgtcaacct gctcaaggac     3840

gacctgcacc ccagctttcc agaggtgtcg ttcttccaca gcgaggagaa caaggctccc     3900

gagagtgagg agctggagat ggagtttgag gacatggaga atgtgcccct ggaccgttcc     3960

tcgcactgtc agagggagga ggcggggggc cgggatggag ggtcctcgct gggtttcaag     4020

cggagctacg aggaacacat cccttacaca cacatgaacg gaggcaagaa aaacgggcgg     4080

attctgacct tgcctcggtc caatccttcc caattggtca ccgacgccaa aaacataaag     4140

aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag     4200

gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag     4260

gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa     4320

cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc     4380

tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt     4440

tataatgaac gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt     4500

tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa     4560

attattatca tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc     4620

acatctcatc tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg     4680

gacaagacaa ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc     4740

gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc     4800

aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga     4860

atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt     4920

gaagaagagc tgtttctgag gagccttcag gattacaaga ttcaaagtgc gctgctggtg     4980

ccaaccctat tctccttctt cgccaaaagc actctgattg acaaatacga tttatctaat     5040

ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg aagtcgggga agcggttgcc     5100

aagaggttcc atctgccagg tatcaggcaa ggatatgggc tcactgagac tacatcagct     5160

attctgatta cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt     5220

tttgaagcga aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc     5280

gaactgtgtg tgagaggtcc tatgattatg tccggttatg taaacaatcc ggaagcgacc     5340

aacgccttga ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa     5400

gacgaacact tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa aggctatcag     5460

gtggctcccg ctgaattgga atccatcttg ctccaacacc ccaacatctt cgacgcaggt     5520

gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag     5580

cacggaaaga cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc     5640

gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga     5700

aaactcgacg caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagatc     5760

gccgtgtaa                                                             5769


<210> 14
<211> 1922
<212> PRT
<213> Artificial Sequence

<220> 
<223> hIR-luc fusion protein

<400> 14
Met Gly Thr Gly Gly Arg Arg Gly Ala Ala Ala Ala Pro Leu Leu Val 
1               5                   10                  15      
Ala Val Ala Ala Leu Leu Leu Gly Ala Ala Gly His Leu Tyr Pro Gly 
            20                  25                  30          
Glu Val Cys Pro Gly Met Asp Ile Arg Asn Asn Leu Thr Arg Leu His 
        35                  40                  45              
Glu Leu Glu Asn Cys Ser Val Ile Glu Gly His Leu Gln Ile Leu Leu 
    50                  55                  60                  
Met Phe Lys Thr Arg Pro Glu Asp Phe Arg Asp Leu Ser Phe Pro Lys 
65                  70                  75                  80  
Leu Ile Met Ile Thr Asp Tyr Leu Leu Leu Phe Arg Val Tyr Gly Leu 
                85                  90                  95      
Glu Ser Leu Lys Asp Leu Phe Pro Asn Leu Thr Val Ile Arg Gly Ser 
            100                 105                 110         
Arg Leu Phe Phe Asn Tyr Ala Leu Val Ile Phe Glu Met Val His Leu 
        115                 120                 125             
Lys Glu Leu Gly Leu Tyr Asn Leu Met Asn Ile Thr Arg Gly Ser Val 
    130                 135                 140                 
Arg Ile Glu Lys Asn Asn Glu Leu Cys Tyr Leu Ala Thr Ile Asp Trp 
145                 150                 155                 160 
Ser Arg Ile Leu Asp Ser Val Glu Asp Asn Tyr Ile Val Leu Asn Lys 
                165                 170                 175     
Asp Asp Asn Glu Glu Cys Gly Asp Ile Cys Pro Gly Thr Ala Lys Gly 
            180                 185                 190         
Lys Thr Asn Cys Pro Ala Thr Val Ile Asn Gly Gln Phe Val Glu Arg 
        195                 200                 205             
Cys Trp Thr His Ser His Cys Gln Lys Val Cys Pro Thr Ile Cys Lys 
    210                 215                 220                 
Ser His Gly Cys Thr Ala Glu Gly Leu Cys Cys His Ser Glu Cys Leu 
225                 230                 235                 240 
Gly Asn Cys Ser Gln Pro Asp Asp Pro Thr Lys Cys Val Ala Cys Arg 
                245                 250                 255     
Asn Phe Tyr Leu Asp Gly Arg Cys Val Glu Thr Cys Pro Pro Pro Tyr 
            260                 265                 270         
Tyr His Phe Gln Asp Trp Arg Cys Val Asn Phe Ser Phe Cys Gln Asp 
        275                 280                 285             
Leu His His Lys Cys Lys Asn Ser Arg Arg Gln Gly Cys His Gln Tyr 
    290                 295                 300                 
Val Ile His Asn Asn Lys Cys Ile Pro Glu Cys Pro Ser Gly Tyr Thr 
305                 310                 315                 320 
Met Asn Ser Ser Asn Leu Leu Cys Thr Pro Cys Leu Gly Pro Cys Pro 
                325                 330                 335     
Lys Val Cys His Leu Leu Glu Gly Glu Lys Thr Ile Asp Ser Val Thr 
            340                 345                 350         
Ser Ala Gln Glu Leu Arg Gly Cys Thr Val Ile Asn Gly Ser Leu Ile 
        355                 360                 365             
Ile Asn Ile Arg Gly Gly Asn Asn Leu Ala Ala Glu Leu Glu Ala Asn 
    370                 375                 380                 
Leu Gly Leu Ile Glu Glu Ile Ser Gly Tyr Leu Lys Ile Arg Arg Ser 
385                 390                 395                 400 
Tyr Ala Leu Val Ser Leu Ser Phe Phe Arg Lys Leu Arg Leu Ile Arg 
                405                 410                 415     
Gly Glu Thr Leu Glu Ile Gly Asn Tyr Ser Phe Tyr Ala Leu Asp Asn 
            420                 425                 430         
Gln Asn Leu Arg Gln Leu Trp Asp Trp Ser Lys His Asn Leu Thr Ile 
        435                 440                 445             
Thr Gln Gly Lys Leu Phe Phe His Tyr Asn Pro Lys Leu Cys Leu Ser 
    450                 455                 460                 
Glu Ile His Lys Met Glu Glu Val Ser Gly Thr Lys Gly Arg Gln Glu 
465                 470                 475                 480 
Arg Asn Asp Ile Ala Leu Lys Thr Asn Gly Asp Gln Ala Ser Cys Glu 
                485                 490                 495     
Asn Glu Leu Leu Lys Phe Ser Tyr Ile Arg Thr Ser Phe Asp Lys Ile 
            500                 505                 510         
Leu Leu Arg Trp Glu Pro Tyr Trp Pro Pro Asp Phe Arg Asp Leu Leu 
        515                 520                 525             
Gly Phe Met Leu Phe Tyr Lys Glu Ala Pro Tyr Gln Asn Val Thr Glu 
    530                 535                 540                 
Phe Asp Gly Gln Asp Ala Cys Gly Ser Asn Ser Trp Thr Val Val Asp 
545                 550                 555                 560 
Ile Asp Pro Pro Leu Arg Ser Asn Asp Pro Lys Ser Gln Asn His Pro 
                565                 570                 575     
Gly Trp Leu Met Arg Gly Leu Lys Pro Trp Thr Gln Tyr Ala Ile Phe 
            580                 585                 590         
Val Lys Thr Leu Val Thr Phe Ser Asp Glu Arg Arg Thr Tyr Gly Ala 
        595                 600                 605             
Lys Ser Asp Ile Ile Tyr Val Gln Thr Asp Ala Thr Asn Pro Ser Val 
    610                 615                 620                 
Pro Leu Asp Pro Ile Ser Val Ser Asn Ser Ser Ser Gln Ile Ile Leu 
625                 630                 635                 640 
Lys Trp Lys Pro Pro Ser Asp Pro Asn Gly Asn Ile Thr His Tyr Leu 
                645                 650                 655     
Val Phe Trp Glu Arg Gln Ala Glu Asp Ser Glu Leu Phe Glu Leu Asp 
            660                 665                 670         
Tyr Cys Leu Lys Gly Leu Lys Leu Pro Ser Arg Thr Trp Ser Pro Pro 
        675                 680                 685             
Phe Glu Ser Glu Asp Ser Gln Lys His Asn Gln Ser Glu Tyr Glu Asp 
    690                 695                 700                 
Ser Ala Gly Glu Cys Cys Ser Cys Pro Lys Thr Asp Ser Gln Ile Leu 
705                 710                 715                 720 
Lys Glu Leu Glu Glu Ser Ser Phe Arg Lys Thr Phe Glu Asp Tyr Leu 
                725                 730                 735     
His Asn Val Val Phe Val Pro Arg Pro Ser Arg Lys Arg Arg Ser Leu 
            740                 745                 750         
Gly Asp Val Gly Asn Val Thr Val Ala Val Pro Thr Val Ala Ala Phe 
        755                 760                 765             
Pro Asn Thr Ser Ser Thr Ser Val Pro Thr Ser Pro Glu Glu His Arg 
    770                 775                 780                 
Pro Phe Glu Lys Val Val Asn Lys Glu Ser Leu Val Ile Ser Gly Leu 
785                 790                 795                 800 
Arg His Phe Thr Gly Tyr Arg Ile Glu Leu Gln Ala Cys Asn Gln Asp 
                805                 810                 815     
Thr Pro Glu Glu Arg Cys Ser Val Ala Ala Tyr Val Ser Ala Arg Thr 
            820                 825                 830         
Met Pro Glu Ala Lys Ala Asp Asp Ile Val Gly Pro Val Thr His Glu 
        835                 840                 845             
Ile Phe Glu Asn Asn Val Val His Leu Met Trp Gln Glu Pro Lys Glu 
    850                 855                 860                 
Pro Asn Gly Leu Ile Val Leu Tyr Glu Val Ser Tyr Arg Arg Tyr Gly 
865                 870                 875                 880 
Asp Glu Glu Leu His Leu Cys Val Ser Arg Lys His Phe Ala Leu Glu 
                885                 890                 895     
Arg Gly Cys Arg Leu Arg Gly Leu Ser Pro Gly Asn Tyr Ser Val Arg 
            900                 905                 910         
Ile Arg Ala Thr Ser Leu Ala Gly Asn Gly Ser Trp Thr Glu Pro Thr 
        915                 920                 925             
Tyr Phe Tyr Val Thr Asp Tyr Leu Asp Val Pro Ser Asn Ile Ala Lys 
    930                 935                 940                 
Ile Ile Ile Gly Pro Leu Ile Phe Val Phe Leu Phe Ser Val Val Ile 
945                 950                 955                 960 
Gly Ser Ile Tyr Leu Phe Leu Arg Lys Arg Gln Pro Asp Gly Pro Leu 
                965                 970                 975     
Gly Pro Leu Tyr Ala Ser Ser Asn Pro Glu Tyr Leu Ser Ala Ser Asp 
            980                 985                 990         
Val Phe Pro Cys Ser Val Tyr Val Pro Asp Glu Trp Glu Val Ser Arg 
        995                 1000                1005            
Glu Lys Ile Thr Leu Leu Arg Glu Leu Gly Gln Gly Ser Phe Gly Met 
    1010                1015                1020                
Val Tyr Glu Gly Asn Ala Arg Asp Ile Ile Lys Gly Glu Ala Glu Thr 
1025                1030                1035                1040
Arg Val Ala Val Lys Thr Val Asn Glu Ser Ala Ser Leu Arg Glu Arg 
                1045                1050                1055    
Ile Glu Phe Leu Asn Glu Ala Ser Val Met Lys Gly Phe Thr Cys His 
            1060                1065                1070        
His Val Val Arg Leu Leu Gly Val Val Ser Lys Gly Gln Pro Thr Leu 
        1075                1080                1085            
Val Val Met Glu Leu Met Ala His Gly Asp Leu Lys Ser Tyr Leu Arg 
    1090                1095                1100                
Ser Leu Arg Pro Glu Ala Glu Asn Asn Pro Gly Arg Pro Pro Pro Thr 
1105                1110                1115                1120
Leu Gln Glu Met Ile Gln Met Ala Ala Glu Ile Ala Asp Gly Met Ala 
                1125                1130                1135    
Tyr Leu Asn Ala Lys Lys Phe Val His Arg Asp Leu Ala Ala Arg Asn 
            1140                1145                1150        
Cys Met Val Ala His Asp Phe Thr Val Lys Ile Gly Asp Phe Gly Met 
        1155                1160                1165            
Thr Arg Asp Ile Tyr Glu Thr Asp Tyr Tyr Arg Lys Gly Gly Lys Gly 
    1170                1175                1180                
Leu Leu Pro Val Arg Trp Met Ala Pro Glu Ser Leu Lys Asp Gly Val 
1185                1190                1195                1200
Phe Thr Thr Ser Ser Asp Met Trp Ser Phe Gly Val Val Leu Trp Glu 
                1205                1210                1215    
Ile Thr Ser Leu Ala Glu Gln Pro Tyr Gln Gly Leu Ser Asn Glu Gln 
            1220                1225                1230        
Val Leu Lys Phe Val Met Asp Gly Gly Tyr Leu Asp Gln Pro Asp Asn 
        1235                1240                1245            
Cys Pro Glu Arg Val Thr Asp Leu Met Arg Met Cys Trp Gln Phe Asn 
    1250                1255                1260                
Pro Lys Met Arg Pro Thr Phe Leu Glu Ile Val Asn Leu Leu Lys Asp 
1265                1270                1275                1280
Asp Leu His Pro Ser Phe Pro Glu Val Ser Phe Phe His Ser Glu Glu 
                1285                1290                1295    
Asn Lys Ala Pro Glu Ser Glu Glu Leu Glu Met Glu Phe Glu Asp Met 
            1300                1305                1310        
Glu Asn Val Pro Leu Asp Arg Ser Ser His Cys Gln Arg Glu Glu Ala 
        1315                1320                1325            
Gly Gly Arg Asp Gly Gly Ser Ser Leu Gly Phe Lys Arg Ser Tyr Glu 
    1330                1335                1340                
Glu His Ile Pro Tyr Thr His Met Asn Gly Gly Lys Lys Asn Gly Arg 
1345                1350                1355                1360
Ile Leu Thr Leu Pro Arg Ser Asn Pro Ser Gln Leu Val Thr Asp Ala 
                1365                1370                1375    
Lys Asn Ile Lys Lys Gly Pro Ala Pro Phe Tyr Pro Leu Glu Asp Gly 
            1380                1385                1390        
Thr Ala Gly Glu Gln Leu His Lys Ala Met Lys Arg Tyr Ala Leu Val 
        1395                1400                1405            
Pro Gly Thr Ile Ala Phe Thr Asp Ala His Ile Glu Val Asp Ile Thr 
    1410                1415                1420                
Tyr Ala Glu Tyr Phe Glu Met Ser Val Arg Leu Ala Glu Ala Met Lys 
1425                1430                1435                1440
Arg Tyr Gly Leu Asn Thr Asn His Arg Ile Val Val Cys Ser Glu Asn 
                1445                1450                1455    
Ser Leu Gln Phe Phe Met Pro Val Leu Gly Ala Leu Phe Ile Gly Val 
            1460                1465                1470        
Ala Val Ala Pro Ala Asn Asp Ile Tyr Asn Glu Arg Glu Leu Leu Asn 
        1475                1480                1485            
Ser Met Gly Ile Ser Gln Pro Thr Val Val Phe Val Ser Lys Lys Gly 
    1490                1495                1500                
Leu Gln Lys Ile Leu Asn Val Gln Lys Lys Leu Pro Ile Ile Gln Lys 
1505                1510                1515                1520
Ile Ile Ile Met Asp Ser Lys Thr Asp Tyr Gln Gly Phe Gln Ser Met 
                1525                1530                1535    
Tyr Thr Phe Val Thr Ser His Leu Pro Pro Gly Phe Asn Glu Tyr Asp 
            1540                1545                1550        
Phe Val Pro Glu Ser Phe Asp Arg Asp Lys Thr Ile Ala Leu Ile Met 
        1555                1560                1565            
Asn Ser Ser Gly Ser Thr Gly Leu Pro Lys Gly Val Ala Leu Pro His 
    1570                1575                1580                
Arg Thr Ala Cys Val Arg Phe Ser His Ala Arg Asp Pro Ile Phe Gly 
1585                1590                1595                1600
Asn Gln Ile Ile Pro Asp Thr Ala Ile Leu Ser Val Val Pro Phe His 
                1605                1610                1615    
His Gly Phe Gly Met Phe Thr Thr Leu Gly Tyr Leu Ile Cys Gly Phe 
            1620                1625                1630        
Arg Val Val Leu Met Tyr Arg Phe Glu Glu Glu Leu Phe Leu Arg Ser 
        1635                1640                1645            
Leu Gln Asp Tyr Lys Ile Gln Ser Ala Leu Leu Val Pro Thr Leu Phe 
    1650                1655                1660                
Ser Phe Phe Ala Lys Ser Thr Leu Ile Asp Lys Tyr Asp Leu Ser Asn 
1665                1670                1675                1680
Leu His Glu Ile Ala Ser Gly Gly Ala Pro Leu Ser Lys Glu Val Gly 
                1685                1690                1695    
Glu Ala Val Ala Lys Arg Phe His Leu Pro Gly Ile Arg Gln Gly Tyr 
            1700                1705                1710        
Gly Leu Thr Glu Thr Thr Ser Ala Ile Leu Ile Thr Pro Glu Gly Asp 
        1715                1720                1725            
Asp Lys Pro Gly Ala Val Gly Lys Val Val Pro Phe Phe Glu Ala Lys 
    1730                1735                1740                
Val Val Asp Leu Asp Thr Gly Lys Thr Leu Gly Val Asn Gln Arg Gly 
1745                1750                1755                1760
Glu Leu Cys Val Arg Gly Pro Met Ile Met Ser Gly Tyr Val Asn Asn 
                1765                1770                1775    
Pro Glu Ala Thr Asn Ala Leu Ile Asp Lys Asp Gly Trp Leu His Ser 
            1780                1785                1790        
Gly Asp Ile Ala Tyr Trp Asp Glu Asp Glu His Phe Phe Ile Val Asp 
        1795                1800                1805            
Arg Leu Lys Ser Leu Ile Lys Tyr Lys Gly Tyr Gln Val Ala Pro Ala 
    1810                1815                1820                
Glu Leu Glu Ser Ile Leu Leu Gln His Pro Asn Ile Phe Asp Ala Gly 
1825                1830                1835                1840
Val Ala Gly Leu Pro Asp Asp Asp Ala Gly Glu Leu Pro Ala Ala Val 
                1845                1850                1855    
Val Val Leu Glu His Gly Lys Thr Met Thr Glu Lys Glu Ile Val Asp 
            1860                1865                1870        
Tyr Val Ala Ser Gln Val Thr Thr Ala Lys Lys Leu Arg Gly Gly Val 
        1875                1880                1885            
Val Phe Val Asp Glu Val Pro Lys Gly Leu Thr Gly Lys Leu Asp Ala 
    1890                1895                1900                
Arg Lys Ile Arg Glu Ile Leu Ile Lys Ala Lys Lys Gly Gly Lys Ile 
1905                1910                1915                1920
Ala Val 
        

<210> 15
<211> 2781
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..2781
<223> /organism="Artificial Sequence"
      /note="extracellular domain of human IGF1R"
      /mol_type="unassigned DNA"

<400> 15
atgaagtctg gctccggagg agggtccccg acctcgctgt gggggctcct gtttctctcc      60

gccgcgctct cgctctggcc gacgagtgga gaaatctgcg ggccaggcat cgacatccgc     120

aacgactatc agcagctgaa gcgcctggag aactgcacgg tgatcgaggg ctacctccac     180

atcctgctca tctccaaggc cgaggactac cgcagctacc gcttccccaa gctcacggtc     240

attaccgagt acttgctgct gttccgagtg gctggcctcg agagcctcgg agacctcttc     300

cccaacctca cggtcatccg cggctggaaa ctcttctaca actacgccct ggtcatcttc     360

gagatgacca atctcaagga tattgggctt tacaacctga ggaacattac tcggggggcc     420

atcaggattg agaaaaatgc tgacctctgt tacctctcca ctgtggactg gtccctgatc     480

ctggatgcgg tgtccaataa ctacattgtg gggaataagc ccccaaagga atgtggggac     540

ctgtgtccag ggaccatgga ggagaagccg atgtgtgaga agaccaccat caacaatgag     600

tacaactacc gctgctggac cacaaaccgc tgccagaaaa tgtgcccaag cacgtgtggg     660

aagcgggcgt gcaccgagaa caatgagtgc tgccaccccg agtgcctggg cagctgcagc     720

gcgcctgaca acgacacggc ctgtgtagct tgccgccact actactatgc cggtgtctgt     780

gtgcctgcct gcccgcccaa cacctacagg tttgagggct ggcgctgtgt ggaccgtgac     840

ttctgcgcca acatcctcag cgccgagagc agcgactccg aggggtttgt gatccacgac     900

ggcgagtgca tgcaggagtg cccctcgggc ttcatccgca acggcagcca gagcatgtac     960

tgcatccctt gtgaaggtcc ttgcccgaag gtctgtgagg aagaaaagaa aacaaagacc    1020

attgattctg ttacttctgc tcagatgctc caaggatgca ccatcttcaa gggcaatttg    1080

ctcattaaca tccgacgggg gaataacatt gcttcagagc tggagaactt catggggctc    1140

atcgaggtgg tgacgggcta cgtgaagatc cgccattctc atgccttggt ctccttgtcc    1200

ttcctaaaaa accttcgcct catcctagga gaggagcagc tagaagggaa ttactccttc    1260

tacgtcctcg acaaccagaa cttgcagcaa ctgtgggact gggaccaccg caacctgacc    1320

atcaaagcag ggaaaatgta ctttgctttc aatcccaaat tatgtgtttc cgaaatttac    1380

cgcatggagg aagtgacggg gactaaaggg cgccaaagca aaggggacat aaacaccagg    1440

aacaacgggg agagagcctc ctgtgaaagt gacgtcctgc atttcacctc caccaccacg    1500

tcgaagaatc gcatcatcat aacctggcac cggtaccggc cccctgacta cagggatctc    1560

atcagcttca ccgtttacta caaggaagca ccctttaaga atgtcacaga gtatgatggg    1620

caggatgcct gcggctccaa cagctggaac atggtggacg tggacctccc gcccaacaag    1680

gacgtggagc ccggcatctt actacatggg ctgaagccct ggactcagta cgccgtttac    1740

gtcaaggctg tgaccctcac catggtggag aacgaccata tccgtggggc caagagtgag    1800

atcttgtaca ttcgcaccaa tgcttcagtt ccttccattc ccttggacgt tctttcagca    1860

tcgaactcct cttctcagtt aatcgtgaag tggaaccctc cctctctgcc caacggcaac    1920

ctgagttact acattgtgcg ctggcagcgg cagcctcagg acggctacct ttaccggcac    1980

aattactgct ccaaagacaa aatccccatc aggaagtatg ccgacggcac catcgacatt    2040

gaggaggtca cagagaaccc caagactgag gtgtgtggtg gggagaaagg gccttgctgc    2100

gcctgcccca aaactgaagc cgagaagcag gccgagaagg aggaggctga ataccgcaaa    2160

gtctttgaga atttcctgca caactccatc ttcgtgccca gacctgaaag gaagcggaga    2220

gatgtcatgc aagtggccaa caccaccatg tccagccgaa gcaggaacac cacggccgca    2280

gacacctaca acatcaccga cccggaagag ctggagacag agtacccttt ctttgagagc    2340

agagtggata acaaggagag aactgtcatt tctaaccttc ggcctttcac attgtaccgc    2400

atcgatatcc acagctgcaa ccacgaggct gagaagctgg gctgcagcgc ctccaacttc    2460

gtctttgcaa ggactatgcc cgcagaagga gcagatgaca ttcctgggcc agtgacctgg    2520

gagccaaggc ctgaaaactc catcttttta aagtggccgg aacctgagaa tcccaatgga    2580

ttgattctaa tgtatgaaat aaaatacgga tcacaagttg aggatcagcg agaatgtgtg    2640

tccagacagg aatacaggaa gtatggaggg gccaagctaa accggctaaa cccggggaac    2700

tacacagccc ggattcaggc cacatctctc tctgggaatg ggtcgtggac agatcctgtg    2760

ttcttctatg tccaggccaa a                                              2781


<210> 16
<211> 927
<212> PRT
<213> Artificial Sequence

<220> 
<223> extracellular domain of human IGF1R

<400> 16
Met Lys Ser Gly Ser Gly Gly Gly Ser Pro Thr Ser Leu Trp Gly Leu 
1               5                   10                  15      
Leu Phe Leu Ser Ala Ala Leu Ser Leu Trp Pro Thr Ser Gly Glu Ile 
            20                  25                  30          
Cys Gly Pro Gly Ile Asp Ile Arg Asn Asp Tyr Gln Gln Leu Lys Arg 
        35                  40                  45              
Leu Glu Asn Cys Thr Val Ile Glu Gly Tyr Leu His Ile Leu Leu Ile 
    50                  55                  60                  
Ser Lys Ala Glu Asp Tyr Arg Ser Tyr Arg Phe Pro Lys Leu Thr Val 
65                  70                  75                  80  
Ile Thr Glu Tyr Leu Leu Leu Phe Arg Val Ala Gly Leu Glu Ser Leu 
                85                  90                  95      
Gly Asp Leu Phe Pro Asn Leu Thr Val Ile Arg Gly Trp Lys Leu Phe 
            100                 105                 110         
Tyr Asn Tyr Ala Leu Val Ile Phe Glu Met Thr Asn Leu Lys Asp Ile 
        115                 120                 125             
Gly Leu Tyr Asn Leu Arg Asn Ile Thr Arg Gly Ala Ile Arg Ile Glu 
    130                 135                 140                 
Lys Asn Ala Asp Leu Cys Tyr Leu Ser Thr Val Asp Trp Ser Leu Ile 
145                 150                 155                 160 
Leu Asp Ala Val Ser Asn Asn Tyr Ile Val Gly Asn Lys Pro Pro Lys 
                165                 170                 175     
Glu Cys Gly Asp Leu Cys Pro Gly Thr Met Glu Glu Lys Pro Met Cys 
            180                 185                 190         
Glu Lys Thr Thr Ile Asn Asn Glu Tyr Asn Tyr Arg Cys Trp Thr Thr 
        195                 200                 205             
Asn Arg Cys Gln Lys Met Cys Pro Ser Thr Cys Gly Lys Arg Ala Cys 
    210                 215                 220                 
Thr Glu Asn Asn Glu Cys Cys His Pro Glu Cys Leu Gly Ser Cys Ser 
225                 230                 235                 240 
Ala Pro Asp Asn Asp Thr Ala Cys Val Ala Cys Arg His Tyr Tyr Tyr 
                245                 250                 255     
Ala Gly Val Cys Val Pro Ala Cys Pro Pro Asn Thr Tyr Arg Phe Glu 
            260                 265                 270         
Gly Trp Arg Cys Val Asp Arg Asp Phe Cys Ala Asn Ile Leu Ser Ala 
        275                 280                 285             
Glu Ser Ser Asp Ser Glu Gly Phe Val Ile His Asp Gly Glu Cys Met 
    290                 295                 300                 
Gln Glu Cys Pro Ser Gly Phe Ile Arg Asn Gly Ser Gln Ser Met Tyr 
305                 310                 315                 320 
Cys Ile Pro Cys Glu Gly Pro Cys Pro Lys Val Cys Glu Glu Glu Lys 
                325                 330                 335     
Lys Thr Lys Thr Ile Asp Ser Val Thr Ser Ala Gln Met Leu Gln Gly 
            340                 345                 350         
Cys Thr Ile Phe Lys Gly Asn Leu Leu Ile Asn Ile Arg Arg Gly Asn 
        355                 360                 365             
Asn Ile Ala Ser Glu Leu Glu Asn Phe Met Gly Leu Ile Glu Val Val 
    370                 375                 380                 
Thr Gly Tyr Val Lys Ile Arg His Ser His Ala Leu Val Ser Leu Ser 
385                 390                 395                 400 
Phe Leu Lys Asn Leu Arg Leu Ile Leu Gly Glu Glu Gln Leu Glu Gly 
                405                 410                 415     
Asn Tyr Ser Phe Tyr Val Leu Asp Asn Gln Asn Leu Gln Gln Leu Trp 
            420                 425                 430         
Asp Trp Asp His Arg Asn Leu Thr Ile Lys Ala Gly Lys Met Tyr Phe 
        435                 440                 445             
Ala Phe Asn Pro Lys Leu Cys Val Ser Glu Ile Tyr Arg Met Glu Glu 
    450                 455                 460                 
Val Thr Gly Thr Lys Gly Arg Gln Ser Lys Gly Asp Ile Asn Thr Arg 
465                 470                 475                 480 
Asn Asn Gly Glu Arg Ala Ser Cys Glu Ser Asp Val Leu His Phe Thr 
                485                 490                 495     
Ser Thr Thr Thr Ser Lys Asn Arg Ile Ile Ile Thr Trp His Arg Tyr 
            500                 505                 510         
Arg Pro Pro Asp Tyr Arg Asp Leu Ile Ser Phe Thr Val Tyr Tyr Lys 
        515                 520                 525             
Glu Ala Pro Phe Lys Asn Val Thr Glu Tyr Asp Gly Gln Asp Ala Cys 
    530                 535                 540                 
Gly Ser Asn Ser Trp Asn Met Val Asp Val Asp Leu Pro Pro Asn Lys 
545                 550                 555                 560 
Asp Val Glu Pro Gly Ile Leu Leu His Gly Leu Lys Pro Trp Thr Gln 
                565                 570                 575     
Tyr Ala Val Tyr Val Lys Ala Val Thr Leu Thr Met Val Glu Asn Asp 
            580                 585                 590         
His Ile Arg Gly Ala Lys Ser Glu Ile Leu Tyr Ile Arg Thr Asn Ala 
        595                 600                 605             
Ser Val Pro Ser Ile Pro Leu Asp Val Leu Ser Ala Ser Asn Ser Ser 
    610                 615                 620                 
Ser Gln Leu Ile Val Lys Trp Asn Pro Pro Ser Leu Pro Asn Gly Asn 
625                 630                 635                 640 
Leu Ser Tyr Tyr Ile Val Arg Trp Gln Arg Gln Pro Gln Asp Gly Tyr 
                645                 650                 655     
Leu Tyr Arg His Asn Tyr Cys Ser Lys Asp Lys Ile Pro Ile Arg Lys 
            660                 665                 670         
Tyr Ala Asp Gly Thr Ile Asp Ile Glu Glu Val Thr Glu Asn Pro Lys 
        675                 680                 685             
Thr Glu Val Cys Gly Gly Glu Lys Gly Pro Cys Cys Ala Cys Pro Lys 
    690                 695                 700                 
Thr Glu Ala Glu Lys Gln Ala Glu Lys Glu Glu Ala Glu Tyr Arg Lys 
705                 710                 715                 720 
Val Phe Glu Asn Phe Leu His Asn Ser Ile Phe Val Pro Arg Pro Glu 
                725                 730                 735     
Arg Lys Arg Arg Asp Val Met Gln Val Ala Asn Thr Thr Met Ser Ser 
            740                 745                 750         
Arg Ser Arg Asn Thr Thr Ala Ala Asp Thr Tyr Asn Ile Thr Asp Pro 
        755                 760                 765             
Glu Glu Leu Glu Thr Glu Tyr Pro Phe Phe Glu Ser Arg Val Asp Asn 
    770                 775                 780                 
Lys Glu Arg Thr Val Ile Ser Asn Leu Arg Pro Phe Thr Leu Tyr Arg 
785                 790                 795                 800 
Ile Asp Ile His Ser Cys Asn His Glu Ala Glu Lys Leu Gly Cys Ser 
                805                 810                 815     
Ala Ser Asn Phe Val Phe Ala Arg Thr Met Pro Ala Glu Gly Ala Asp 
            820                 825                 830         
Asp Ile Pro Gly Pro Val Thr Trp Glu Pro Arg Pro Glu Asn Ser Ile 
        835                 840                 845             
Phe Leu Lys Trp Pro Glu Pro Glu Asn Pro Asn Gly Leu Ile Leu Met 
    850                 855                 860                 
Tyr Glu Ile Lys Tyr Gly Ser Gln Val Glu Asp Gln Arg Glu Cys Val 
865                 870                 875                 880 
Ser Arg Gln Glu Tyr Arg Lys Tyr Gly Gly Ala Lys Leu Asn Arg Leu 
                885                 890                 895     
Asn Pro Gly Asn Tyr Thr Ala Arg Ile Gln Ala Thr Ser Leu Ser Gly 
            900                 905                 910         
Asn Gly Ser Trp Thr Asp Pro Val Phe Phe Tyr Val Gln Ala Lys 
        915                 920                 925         

<210> 17
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..33
<223> /organism="Artificial Sequence"
      /note="Primer P5"
      /mol_type="unassigned DNA"

<400> 17
acggttaaca tgggcaccgg gggccggcgg ggg                                  33


<210> 18
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..33
<223> /organism="Artificial Sequence"
      /note="Primer P6"
      /mol_type="unassigned DNA"

<400> 18
acgcaattgc agcaagatct tgtcaaaaga tgt                                  33


<210> 19
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..36
<223> /organism="Artificial Sequence"
      /note="Primer P7"
      /mol_type="unassigned DNA"

<400> 19
atcggatcca ccatgaagtc tggctccgga ggaggg                               36


<210> 20
<211> 83
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..83
<223> /organism="Artificial Sequence"
      /note="Primer P8"
      /mol_type="unassigned DNA"

<400> 20
atcaagcttc actttcctgc tcctggttgc tgattctctg aaagctcact gctgccaagt     60

ttggcctgga catagaagaa cac                                             83


