               SEQUENCE LISTING

<110> Novartis AG

<120> Pilus Proteins and Compositions

<130> PAT054771-WO-PCT

<160> 118

<170> BiSSAP 1.2

<210> 1
<211> 305
<212> PRT
<213> Streptococcus agalactiae


<400> 1
Met Gly Gln Lys Ser Lys Ile Ser Leu Ala Thr Asn Ile Arg Ile Trp 
1               5                   10                  15      
Ile Phe Arg Leu Ile Phe Leu Ala Gly Phe Leu Val Leu Ala Phe Pro 
            20                  25                  30          
Ile Val Ser Gln Val Met Tyr Phe Gln Ala Ser His Ala Asn Ile Asn 
        35                  40                  45              
Ala Phe Lys Glu Ala Val Thr Lys Ile Asp Arg Val Glu Ile Asn Arg 
    50                  55                  60                  
Arg Leu Glu Leu Ala Tyr Ala Tyr Asn Ala Ser Ile Ala Gly Ala Lys 
65                  70                  75                  80  
Thr Asn Gly Glu Tyr Pro Ala Leu Lys Asp Pro Tyr Ser Ala Glu Gln 
                85                  90                  95      
Lys Gln Ala Gly Val Val Glu Tyr Ala Arg Met Leu Glu Val Lys Glu 
            100                 105                 110         
Gln Ile Gly His Val Ile Ile Pro Arg Ile Asn Gln Asp Ile Pro Ile 
        115                 120                 125             
Tyr Ala Gly Ser Ala Glu Glu Asn Leu Gln Arg Gly Val Gly His Leu 
    130                 135                 140                 
Glu Gly Thr Ser Leu Pro Val Gly Gly Glu Ser Thr His Ala Val Leu 
145                 150                 155                 160 
Thr Ala His Arg Gly Leu Pro Thr Ala Lys Leu Phe Thr Asn Leu Asp 
                165                 170                 175     
Lys Val Thr Val Gly Asp Arg Phe Tyr Ile Glu His Ile Gly Gly Lys 
            180                 185                 190         
Ile Ala Tyr Gln Val Asp Gln Ile Lys Val Ile Ala Pro Asp Gln Leu 
        195                 200                 205             
Glu Asp Leu Tyr Val Ile Gln Gly Glu Asp His Val Thr Leu Leu Thr 
    210                 215                 220                 
Cys Thr Pro Tyr Met Ile Asn Ser His Arg Leu Leu Val Arg Gly Lys 
225                 230                 235                 240 
Arg Ile Pro Tyr Val Glu Lys Thr Val Gln Lys Asp Ser Lys Thr Phe 
                245                 250                 255     
Arg Gln Gln Gln Tyr Leu Thr Tyr Ala Met Trp Val Val Val Gly Leu 
            260                 265                 270         
Ile Leu Leu Ser Leu Leu Ile Trp Phe Lys Lys Thr Lys Gln Lys Lys 
        275                 280                 285             
Arg Arg Lys Asn Glu Lys Ala Ala Ser Gln Asn Ser His Asn Asn Ser 
    290                 295                 300                 
Lys 
305 

<210> 2
<211> 283
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS Sortase C2 of PI-1

<400> 2
Met Lys Lys Arg Leu Val Lys Ile Val Thr Ile Ile Arg Asn Asn Lys 
1               5                   10                  15      
Ile Arg Thr Leu Ile Phe Val Met Gly Ser Leu Ile Leu Leu Phe Pro 
            20                  25                  30          
Ile Val Ser Gln Val Ser Tyr Tyr Leu Ala Ser His Gln Asn Ile Asn 
        35                  40                  45              
Gln Phe Lys Arg Glu Val Ala Lys Ile Asp Thr Asn Thr Val Glu Arg 
    50                  55                  60                  
Arg Ile Ala Leu Ala Asn Ala Tyr Asn Glu Thr Leu Ser Arg Asn Pro 
65                  70                  75                  80  
Leu Leu Ile Asp Pro Phe Thr Ser Lys Gln Lys Glu Gly Leu Arg Glu 
                85                  90                  95      
Tyr Ala Arg Met Leu Glu Val His Glu Gln Ile Gly His Val Ala Ile 
            100                 105                 110         
Pro Ser Ile Gly Val Asp Ile Pro Ile Tyr Ala Gly Thr Ser Glu Thr 
        115                 120                 125             
Val Leu Gln Lys Gly Ser Gly His Leu Glu Gly Thr Ser Leu Pro Val 
    130                 135                 140                 
Gly Gly Leu Ser Thr His Ser Val Leu Thr Ala His Arg Gly Leu Pro 
145                 150                 155                 160 
Thr Ala Arg Leu Phe Thr Asp Leu Asn Lys Val Lys Lys Gly Gln Ile 
                165                 170                 175     
Phe Tyr Val Thr Asn Ile Lys Glu Thr Leu Ala Tyr Lys Val Val Ser 
            180                 185                 190         
Ile Lys Val Val Asp Pro Thr Ala Leu Ser Glu Val Lys Ile Val Asn 
        195                 200                 205             
Gly Lys Asp Tyr Ile Thr Leu Leu Thr Cys Thr Pro Tyr Met Ile Asn 
    210                 215                 220                 
Ser His Arg Leu Leu Val Lys Gly Glu Arg Ile Pro Tyr Asp Ser Thr 
225                 230                 235                 240 
Glu Ala Glu Lys His Lys Glu Gln Thr Val Gln Asp Tyr Arg Leu Ser 
                245                 250                 255     
Leu Val Leu Lys Ile Leu Leu Val Leu Leu Ile Gly Leu Phe Ile Val 
            260                 265                 270         
Ile Met Met Arg Arg Trp Met Gln His Arg Gln 
        275                 280             

<210> 3
<211> 292
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS Sortas C1 of PI-2a

<400> 3
Met Lys Thr Lys Lys Ile Ile Lys Lys Thr Lys Lys Lys Lys Ser Asn 
1               5                   10                  15      
Leu Pro Phe Ile Ile Leu Phe Leu Ile Gly Leu Ser Ile Leu Leu Tyr 
            20                  25                  30          
Pro Val Val Ser Arg Phe Tyr Tyr Thr Ile Glu Ser Asn Asn Gln Thr 
        35                  40                  45              
Gln Asp Phe Glu Arg Ala Ala Lys Lys Leu Ser Gln Lys Glu Ile Asn 
    50                  55                  60                  
Arg Arg Met Ala Leu Ala Gln Ala Tyr Asn Asp Ser Leu Asn Asn Val 
65                  70                  75                  80  
His Leu Glu Asp Pro Tyr Glu Lys Lys Arg Ile Gln Lys Gly Ile Ala 
                85                  90                  95      
Glu Tyr Ala Arg Met Leu Glu Val Ser Glu Lys Ile Gly Ile Ile Ser 
            100                 105                 110         
Val Pro Lys Ile Gly Gln Lys Leu Pro Ile Phe Ala Gly Ser Ser Gln 
        115                 120                 125             
Glu Val Leu Ser Lys Gly Ala Gly His Leu Glu Gly Thr Ser Leu Pro 
    130                 135                 140                 
Ile Gly Gly Asn Ser Thr His Thr Val Ile Thr Ala His Ser Gly Ile 
145                 150                 155                 160 
Pro Asp Lys Glu Leu Phe Ser Asn Leu Lys Lys Leu Lys Lys Gly Asp 
                165                 170                 175     
Lys Phe Tyr Ile Gln Asn Ile Lys Glu Thr Ile Ala Tyr Gln Val Asp 
            180                 185                 190         
Gln Ile Lys Val Val Thr Pro Asp Asn Phe Ser Asp Leu Leu Val Val 
        195                 200                 205             
Pro Gly His Asp Tyr Ala Thr Leu Leu Thr Cys Thr Pro Ile Met Val 
    210                 215                 220                 
Asn Thr His Arg Leu Leu Val Arg Gly His Arg Ile Pro Tyr Lys Gly 
225                 230                 235                 240 
Pro Ile Asp Glu Lys Leu Ile Lys Asp Gly His Leu Asn Thr Ile Tyr 
                245                 250                 255     
Arg Tyr Leu Phe Tyr Ile Ser Leu Val Ile Ile Ala Trp Leu Leu Trp 
            260                 265                 270         
Leu Ile Lys Arg Gln Arg Gln Lys Asn Arg Leu Ser Ser Val Arg Lys 
        275                 280                 285             
Gly Ile Glu Ser 
    290         

<210> 4
<211> 294
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS Sortase C2 of PI-2a

<400> 4
Met Arg Gly Lys Phe Gln Lys Asn Leu Lys Lys Ser Val Val Leu Asn 
1               5                   10                  15      
Arg Trp Met Asn Ile Gly Leu Ile Leu Leu Phe Leu Val Gly Leu Leu 
            20                  25                  30          
Ile Thr Ser Tyr Pro Phe Ile Ser Asn Trp Tyr Tyr Asn Ile Lys Ala 
        35                  40                  45              
Asn Asn Gln Val Thr Asn Phe Asp Asn Gln Thr Gln Lys Leu Asn Ala 
    50                  55                  60                  
Lys Glu Ile Asn Arg Arg Phe Glu Leu Ala Lys Ala Tyr Asn Arg Thr 
65                  70                  75                  80  
Leu Asp Pro Ser Arg Leu Ser Asp Pro Tyr Thr Glu Lys Glu Lys Lys 
                85                  90                  95      
Gly Ile Ala Glu Tyr Ala His Met Leu Glu Ile Thr Glu Met Ile Gly 
            100                 105                 110         
Tyr Ile Asp Ile Pro Ser Ile Lys Gln Lys Leu Pro Ile Tyr Ala Gly 
        115                 120                 125             
Thr Thr Ser Ser Val Leu Glu Lys Gly Ser Gly His Leu Glu Gly Thr 
    130                 135                 140                 
Ser Leu Pro Ile Gly Gly Lys Ser Ser His Thr Val Ile Thr Ala His 
145                 150                 155                 160 
Arg Gly Leu Pro Lys Ala Lys Leu Phe Thr Asp Leu Asp Lys Leu Lys 
                165                 170                 175     
Lys Gly Lys Ile Phe Tyr Ile His Asn Ile Lys Glu Val Leu Ala Tyr 
            180                 185                 190         
Lys Val Asp Gln Ile Ser Val Val Lys Pro Asp Asn Phe Ser Lys Leu 
        195                 200                 205             
Leu Val Val Lys Gly Lys Asp Tyr Ala Thr Leu Leu Thr Cys Thr Pro 
    210                 215                 220                 
Tyr Ser Ile Asn Ser His Arg Leu Leu Val Arg Gly His Arg Ile Lys 
225                 230                 235                 240 
Tyr Val Pro Pro Val Lys Glu Lys Asn Tyr Leu Met Lys Glu Leu Gln 
                245                 250                 255     
Thr His Tyr Lys Leu Tyr Phe Leu Leu Ser Ile Leu Val Ile Leu Ile 
            260                 265                 270         
Leu Val Ala Leu Leu Leu Tyr Leu Lys Arg Lys Phe Lys Glu Arg Lys 
        275                 280                 285             
Arg Lys Gly Asn Gln Lys 
    290                 

<210> 5
<211> 268
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS Sortase C1 of PI-2b

<400> 5
Met Ala Tyr Pro Ser Leu Ala Asn Tyr Trp Asn Ser Phe His Gln Ser 
1               5                   10                  15      
Arg Ala Ile Met Asp Tyr Gln Asp Arg Val Thr His Met Asp Glu Asn 
            20                  25                  30          
Asp Tyr Lys Lys Ile Ile Asn Arg Ala Lys Glu Tyr Asn Lys Gln Phe 
        35                  40                  45              
Lys Thr Ser Gly Met Lys Trp His Met Thr Ser Gln Glu Arg Leu Asp 
    50                  55                  60                  
Tyr Asn Ser Gln Leu Ala Ile Asp Lys Thr Gly Asn Met Gly Tyr Ile 
65                  70                  75                  80  
Ser Ile Pro Lys Ile Asn Ile Lys Leu Pro Leu Tyr His Gly Thr Ser 
                85                  90                  95      
Glu Lys Val Leu Gln Thr Ser Ile Gly His Leu Glu Gly Ser Ser Leu 
            100                 105                 110         
Pro Ile Gly Gly Asp Ser Thr His Ser Ile Leu Ser Gly His Arg Gly 
        115                 120                 125             
Leu Pro Ser Ser Arg Leu Phe Ser Asp Leu Asp Lys Leu Lys Val Gly 
    130                 135                 140                 
Asp His Trp Thr Val Ser Ile Leu Asn Glu Thr Tyr Thr Tyr Gln Val 
145                 150                 155                 160 
Asp Gln Ile Arg Thr Val Lys Pro Asp Asp Leu Arg Asp Leu Gln Ile 
                165                 170                 175     
Val Lys Gly Lys Asp Tyr Gln Thr Leu Val Thr Cys Thr Pro Tyr Gly 
            180                 185                 190         
Val Asn Thr His Arg Leu Leu Val Arg Gly His Arg Val Pro Asn Asp 
        195                 200                 205             
Asn Gly Asn Ala Leu Val Val Ala Glu Ala Ile Gln Ile Glu Pro Ile 
    210                 215                 220                 
Tyr Ile Ala Pro Phe Ile Ala Ile Phe Leu Thr Leu Ile Leu Leu Leu 
225                 230                 235                 240 
Ile Ser Leu Glu Val Thr Arg Arg Ala Arg Gln Arg Lys Lys Ile Leu 
                245                 250                 255     
Lys Gln Ala Met Arg Lys Glu Glu Asn Asn Asp Leu 
            260                 265             

<210> 6
<211> 279
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus Sortase C1

<400> 6
Met Ala Val Met Ala Tyr Pro Leu Val Ser Arg Leu Tyr Tyr Arg Val 
1               5                   10                  15      
Glu Ser Asn Gln Gln Ile Ala Asp Phe Asp Lys Glu Lys Ala Thr Leu 
            20                  25                  30          
Asp Glu Ala Asp Ile Asp Glu Arg Met Lys Leu Ala Gln Ala Phe Asn 
        35                  40                  45              
Asp Ser Leu Asn Asn Val Val Ser Gly Asp Pro Trp Ser Glu Glu Met 
    50                  55                  60                  
Lys Lys Lys Gly Arg Ala Glu Tyr Ala Arg Met Leu Glu Ile His Glu 
65                  70                  75                  80  
Arg Met Gly His Val Glu Ile Pro Val Ile Asp Val Asp Leu Pro Val 
                85                  90                  95      
Tyr Ala Gly Thr Ala Glu Glu Val Leu Gln Gln Gly Ala Gly His Leu 
            100                 105                 110         
Glu Gly Thr Ser Leu Pro Ile Gly Gly Asn Ser Thr His Ala Val Ile 
        115                 120                 125             
Thr Ala His Thr Gly Leu Pro Thr Ala Lys Met Phe Thr Asp Leu Thr 
    130                 135                 140                 
Lys Leu Lys Val Gly Asp Lys Phe Tyr Val His Asn Ile Lys Glu Val 
145                 150                 155                 160 
Met Ala Tyr Gln Val Asp Gln Val Lys Val Ile Glu Pro Thr Asn Phe 
                165                 170                 175     
Asp Asp Leu Leu Ile Val Pro Gly His Asp Tyr Val Thr Leu Leu Thr 
            180                 185                 190         
Cys Thr Pro Tyr Met Ile Asn Thr His Arg Leu Leu Val Arg Gly His 
        195                 200                 205             
Arg Ile Pro Tyr Val Ala Glu Val Glu Glu Glu Phe Ile Ala Ala Asn 
    210                 215                 220                 
Lys Leu Ser His Leu Tyr Arg Tyr Leu Phe Tyr Val Ala Val Gly Leu 
225                 230                 235                 240 
Ile Val Ile Leu Leu Trp Ile Ile Arg Arg Leu Arg Lys Lys Lys Lys 
                245                 250                 255     
Gln Pro Glu Lys Ala Leu Lys Ala Leu Lys Ala Ala Arg Lys Glu Val 
            260                 265                 270         
Lys Val Glu Asp Gly Gln Gln 
        275                 

<210> 7
<211> 261
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus Sortase C2

<400> 7
Met Ser Arg Tyr Tyr Tyr Arg Ile Glu Ser Asn Glu Val Ile Lys Glu 
1               5                   10                  15      
Phe Asp Glu Thr Val Ser Gln Met Asp Lys Ala Glu Leu Glu Glu Arg 
            20                  25                  30          
Trp Arg Leu Ala Gln Ala Phe Asn Ala Thr Leu Lys Pro Ser Glu Ile 
        35                  40                  45              
Leu Asp Pro Phe Thr Glu Gln Glu Lys Lys Lys Gly Val Ser Glu Tyr 
    50                  55                  60                  
Ala Asn Met Leu Lys Val His Glu Arg Ile Gly Tyr Val Glu Ile Pro 
65                  70                  75                  80  
Ala Ile Asp Gln Glu Ile Pro Met Tyr Val Gly Thr Ser Glu Asp Ile 
                85                  90                  95      
Leu Gln Lys Gly Ala Gly Leu Leu Glu Gly Ala Ser Leu Pro Val Gly 
            100                 105                 110         
Gly Glu Asn Thr His Thr Val Ile Thr Ala His Arg Gly Leu Pro Thr 
        115                 120                 125             
Ala Glu Leu Phe Ser Gln Leu Asp Lys Met Lys Lys Gly Asp Ile Phe 
    130                 135                 140                 
Tyr Leu His Val Leu Asp Gln Val Leu Ala Tyr Gln Val Asp Gln Ile 
145                 150                 155                 160 
Val Thr Val Glu Pro Asn Asp Phe Glu Pro Val Leu Ile Gln His Gly 
                165                 170                 175     
Glu Asp Tyr Ala Thr Leu Leu Thr Cys Thr Pro Tyr Met Ile Asn Ser 
            180                 185                 190         
His Arg Leu Leu Val Arg Gly Lys Arg Ile Pro Tyr Thr Ala Pro Ile 
        195                 200                 205             
Ala Glu Arg Asn Arg Ala Val Arg Glu Arg Gly Gln Phe Trp Leu Trp 
    210                 215                 220                 
Leu Leu Leu Gly Ala Met Ala Val Ile Leu Leu Leu Leu Tyr Arg Val 
225                 230                 235                 240 
Tyr Arg Asn Arg Arg Ile Val Lys Gly Leu Glu Lys Gln Leu Glu Gly 
                245                 250                 255     
Arg His Val Lys Asp 
            260     

<210> 8
<211> 283
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus Sortase C3

<400> 8
Met Ser Arg Thr Lys Leu Arg Ala Leu Leu Gly Tyr Leu Leu Met Leu 
1               5                   10                  15      
Val Ala Cys Leu Ile Pro Ile Tyr Cys Phe Gly Gln Met Val Leu Gln 
            20                  25                  30          
Ser Leu Gly Gln Val Lys Gly His Ala Thr Phe Val Lys Ser Met Thr 
        35                  40                  45              
Thr Glu Met Tyr Gln Glu Gln Gln Asn His Ser Leu Ala Tyr Asn Gln 
    50                  55                  60                  
Arg Leu Ala Ser Gln Asn Arg Ile Val Asp Pro Phe Leu Ala Glu Gly 
65                  70                  75                  80  
Tyr Glu Val Asn Tyr Gln Val Ser Asp Asp Pro Asp Ala Val Tyr Gly 
                85                  90                  95      
Tyr Leu Ser Ile Pro Ser Leu Glu Ile Met Glu Pro Val Tyr Leu Gly 
            100                 105                 110         
Ala Asp Tyr His His Leu Gly Met Gly Leu Ala His Val Asp Gly Thr 
        115                 120                 125             
Pro Leu Pro Leu Asp Gly Thr Gly Ile Arg Ser Val Ile Ala Gly His 
    130                 135                 140                 
Arg Ala Glu Pro Ser His Val Phe Phe Arg His Leu Asp Gln Leu Lys 
145                 150                 155                 160 
Val Gly Asp Ala Leu Tyr Tyr Asp Asn Gly Gln Glu Ile Val Glu Tyr 
                165                 170                 175     
Gln Met Met Asp Thr Glu Ile Ile Leu Pro Ser Glu Trp Glu Lys Leu 
            180                 185                 190         
Glu Ser Val Ser Ser Lys Asn Ile Met Thr Leu Ile Thr Cys Asp Pro 
        195                 200                 205             
Ile Pro Thr Phe Asn Lys Arg Leu Leu Val Asn Phe Glu Arg Val Ala 
    210                 215                 220                 
Val Tyr Gln Lys Ser Asp Pro Gln Thr Ala Ala Val Ala Arg Val Ala 
225                 230                 235                 240 
Phe Thr Lys Glu Gly Gln Ser Val Ser Arg Val Ala Thr Ser Gln Trp 
                245                 250                 255     
Leu Tyr Arg Gly Leu Val Val Leu Ala Phe Leu Gly Ile Leu Phe Val 
            260                 265                 270         
Leu Trp Lys Leu Ala Arg Leu Leu Arg Gly Lys 
        275                 280             

<210> 9
<211> 251
<212> PRT
<213> Streptococcus pyogenes

<220> 
<223> GAS Sortase C1

<400> 9
Met Glu Cys Tyr Arg Asp Arg Gln Leu Leu Ser Thr Tyr His Lys Gln 
1               5                   10                  15      
Val Thr Gln Lys Lys Pro Ser Glu Met Glu Glu Val Trp Gln Lys Ala 
            20                  25                  30          
Lys Ala Tyr Asn Ala Arg Leu Gly Ile Gln Pro Val Pro Asp Ala Phe 
        35                  40                  45              
Ser Phe Arg Asp Gly Ile His Asp Lys Asn Tyr Glu Ser Leu Leu Gln 
    50                  55                  60                  
Ile Glu Asn Asn Asp Ile Met Gly Tyr Val Glu Val Pro Ser Ile Lys 
65                  70                  75                  80  
Val Thr Leu Pro Ile Tyr His Tyr Thr Thr Asp Glu Val Leu Thr Lys 
                85                  90                  95      
Gly Ala Gly His Leu Phe Gly Ser Ala Leu Pro Val Gly Gly Asp Gly 
            100                 105                 110         
Thr His Thr Val Ile Ser Ala His Arg Gly Leu Pro Ser Ala Glu Met 
        115                 120                 125             
Phe Thr Asn Leu Asn Leu Val Lys Lys Gly Asp Thr Phe Tyr Phe Arg 
    130                 135                 140                 
Val Leu Asn Lys Val Leu Ala Tyr Lys Val Asp Gln Ile Leu Thr Val 
145                 150                 155                 160 
Glu Pro Asp Gln Val Thr Ser Leu Ser Gly Val Met Gly Lys Asp Tyr 
                165                 170                 175     
Ala Thr Leu Val Thr Cys Thr Pro Tyr Gly Val Asn Thr Lys Arg Leu 
            180                 185                 190         
Leu Val Arg Gly His Arg Ile Ala Tyr His Tyr Lys Lys Tyr Gln Gln 
        195                 200                 205             
Ala Lys Lys Ala Met Lys Leu Val Asp Lys Ser Arg Met Trp Ala Glu 
    210                 215                 220                 
Val Val Cys Ala Ala Phe Gly Val Val Ile Ala Ile Ile Leu Val Phe 
225                 230                 235                 240 
Met Tyr Ser Arg Val Ser Ala Lys Lys Ser Lys 
                245                 250     

<210> 10
<211> 256
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS Sortase C1 of PI-1 deletion mutant

<400> 10
Ile Val Ser Gln Val Met Tyr Phe Gln Ala Ser His Ala Asn Ile Asn 
1               5                   10                  15      
Ala Phe Lys Glu Ala Val Thr Lys Ile Asp Arg Val Glu Ile Asn Arg 
            20                  25                  30          
Arg Leu Glu Leu Ala Tyr Ala Tyr Asn Ala Ser Ile Ala Gly Ala Lys 
        35                  40                  45              
Thr Asn Gly Glu Tyr Glu Tyr Ala Arg Met Leu Glu Val Lys Glu Gln 
    50                  55                  60                  
Ile Gly His Val Ile Ile Pro Arg Ile Asn Gln Asp Ile Pro Ile Tyr 
65                  70                  75                  80  
Ala Gly Ser Ala Glu Glu Asn Leu Gln Arg Gly Val Gly His Leu Glu 
                85                  90                  95      
Gly Thr Ser Leu Pro Val Gly Gly Glu Ser Thr His Ala Val Leu Thr 
            100                 105                 110         
Ala His Arg Gly Leu Pro Thr Ala Lys Leu Phe Thr Asn Leu Asp Lys 
        115                 120                 125             
Val Thr Val Gly Asp Arg Phe Tyr Ile Glu His Ile Gly Gly Lys Ile 
    130                 135                 140                 
Ala Tyr Gln Val Asp Gln Ile Lys Val Ile Ala Pro Asp Gln Leu Glu 
145                 150                 155                 160 
Asp Leu Tyr Val Ile Gln Gly Glu Asp His Val Thr Leu Leu Thr Cys 
                165                 170                 175     
Thr Pro Tyr Met Ile Asn Ser His Arg Leu Leu Val Arg Gly Lys Arg 
            180                 185                 190         
Ile Pro Tyr Val Glu Lys Thr Val Gln Lys Asp Ser Lys Thr Phe Arg 
        195                 200                 205             
Gln Gln Gln Tyr Leu Thr Tyr Ala Met Trp Val Val Val Gly Leu Ile 
    210                 215                 220                 
Leu Leu Ser Leu Leu Ile Trp Phe Lys Lys Thr Lys Gln Lys Lys Arg 
225                 230                 235                 240 
Arg Lys Asn Glu Lys Ala Ala Ser Gln Asn Ser His Asn Asn Ser Lys 
                245                 250                 255     



<210> 11
<211> 225
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS Sortase C2 of PI-1 deletion mutant

<400> 11
Ala Ser His Gln Asn Ile Asn Gln Phe Lys Arg Glu Val Ala Lys Ile 
1               5                   10                  15      
Asp Thr Asn Thr Val Glu Arg Arg Ile Ala Leu Ala Asn Ala Tyr Asn 
            20                  25                  30          
Glu Thr Leu Ser Arg Glu Tyr Ala Arg Met Leu Glu Val His Glu Gln 
        35                  40                  45              
Ile Gly His Val Ala Ile Pro Ser Ile Gly Val Asp Ile Pro Ile Tyr 
    50                  55                  60                  
Ala Gly Thr Ser Glu Thr Val Leu Gln Lys Gly Ser Gly His Leu Glu 
65                  70                  75                  80  
Gly Thr Ser Leu Pro Val Gly Gly Leu Ser Thr His Ser Val Leu Thr 
                85                  90                  95      
Ala His Arg Gly Leu Pro Thr Ala Arg Leu Phe Thr Asp Leu Asn Lys 
            100                 105                 110         
Val Lys Lys Gly Gln Ile Phe Tyr Val Thr Asn Ile Lys Glu Thr Leu 
        115                 120                 125             
Ala Tyr Lys Val Val Ser Ile Lys Val Val Asp Pro Thr Ala Leu Ser 
    130                 135                 140                 
Glu Val Lys Ile Val Asn Gly Lys Asp Tyr Ile Thr Leu Leu Thr Cys 
145                 150                 155                 160 
Thr Pro Tyr Met Ile Asn Ser His Arg Leu Leu Val Lys Gly Glu Arg 
                165                 170                 175     
Ile Pro Tyr Asp Ser Thr Glu Ala Glu Lys His Lys Glu Gln Thr Val 
            180                 185                 190         
Gln Asp Tyr Arg Leu Ser Leu Val Leu Lys Ile Leu Leu Val Leu Leu 
        195                 200                 205             
Ile Gly Leu Phe Ile Val Ile Met Met Arg Arg Trp Met Gln His Arg 
    210                 215                 220                 
Gln 
225 

<210> 12
<211> 234
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS Sortase C1 of PI-2a deletion mutant

<400> 12
Glu Ser Asn Asn Gln Thr Gln Asp Phe Glu Arg Ala Ala Lys Lys Leu 
1               5                   10                  15      
Ser Gln Lys Glu Ile Asn Arg Arg Met Ala Leu Ala Gln Ala Tyr Asn 
            20                  25                  30          
Asp Ser Leu Asn Asn Val Glu Tyr Ala Arg Met Leu Glu Val Ser Glu 
        35                  40                  45              
Lys Ile Gly Ile Ile Ser Val Pro Lys Ile Gly Gln Lys Leu Pro Ile 
    50                  55                  60                  
Phe Ala Gly Ser Ser Gln Glu Val Leu Ser Lys Gly Ala Gly His Leu 
65                  70                  75                  80  
Glu Gly Thr Ser Leu Pro Ile Gly Gly Asn Ser Thr His Thr Val Ile 
                85                  90                  95      
Thr Ala His Ser Gly Ile Pro Asp Lys Glu Leu Phe Ser Asn Leu Lys 
            100                 105                 110         
Lys Leu Lys Lys Gly Asp Lys Phe Tyr Ile Gln Asn Ile Lys Glu Thr 
        115                 120                 125             
Ile Ala Tyr Gln Val Asp Gln Ile Lys Val Val Thr Pro Asp Asn Phe 
    130                 135                 140                 
Ser Asp Leu Leu Val Val Pro Gly His Asp Tyr Ala Thr Leu Leu Thr 
145                 150                 155                 160 
Cys Thr Pro Ile Met Val Asn Thr His Arg Leu Leu Val Arg Gly His 
                165                 170                 175     
Arg Ile Pro Tyr Lys Gly Pro Ile Asp Glu Lys Leu Ile Lys Asp Gly 
            180                 185                 190         
His Leu Asn Thr Ile Tyr Arg Tyr Leu Phe Tyr Ile Ser Leu Val Ile 
        195                 200                 205             
Ile Ala Trp Leu Leu Trp Leu Ile Lys Arg Gln Arg Gln Lys Asn Arg 
    210                 215                 220                 
Leu Ser Ser Val Arg Lys Gly Ile Glu Ser 
225                 230                 

<210> 13
<211> 232
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS Sortase C2 of PI-2a deletion mutant

<400> 13
Lys Ala Asn Asn Gln Val Thr Asn Phe Asp Asn Gln Thr Gln Lys Leu 
1               5                   10                  15      
Asn Ala Lys Glu Ile Asn Arg Arg Phe Glu Leu Ala Lys Ala Tyr Asn 
            20                  25                  30          
Arg Thr Leu Asp Pro Glu Tyr Ala His Met Leu Glu Ile Thr Glu Met 
        35                  40                  45              
Ile Gly Tyr Ile Asp Ile Pro Ser Ile Lys Gln Lys Leu Pro Ile Tyr 
    50                  55                  60                  
Ala Gly Thr Thr Ser Ser Val Leu Glu Lys Gly Ser Gly His Leu Glu 
65                  70                  75                  80  
Gly Thr Ser Leu Pro Ile Gly Gly Lys Ser Ser His Thr Val Ile Thr 
                85                  90                  95      
Ala His Arg Gly Leu Pro Lys Ala Lys Leu Phe Thr Asp Leu Asp Lys 
            100                 105                 110         
Leu Lys Lys Gly Lys Ile Phe Tyr Ile His Asn Ile Lys Glu Val Leu 
        115                 120                 125             
Ala Tyr Lys Val Asp Gln Ile Ser Val Val Lys Pro Asp Asn Phe Ser 
    130                 135                 140                 
Lys Leu Leu Val Val Lys Gly Lys Asp Tyr Ala Thr Leu Leu Thr Cys 
145                 150                 155                 160 
Thr Pro Tyr Ser Ile Asn Ser His Arg Leu Leu Val Arg Gly His Arg 
                165                 170                 175     
Ile Lys Tyr Val Pro Pro Val Lys Glu Lys Asn Tyr Leu Met Lys Glu 
            180                 185                 190         
Leu Gln Thr His Tyr Lys Leu Tyr Phe Leu Leu Ser Ile Leu Val Ile 
        195                 200                 205             
Leu Ile Leu Val Ala Leu Leu Leu Tyr Leu Lys Arg Lys Phe Lys Glu 
    210                 215                 220                 
Arg Lys Arg Lys Gly Asn Gln Lys 
225                 230         

<210> 14
<211> 238
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS Sortase C1 of PI-2b deletion mutant

<400> 14
His Gln Ser Arg Ala Ile Met Asp Tyr Gln Asp Arg Val Thr His Met 
1               5                   10                  15      
Asp Glu Asn Asp Tyr Lys Lys Ile Ile Asn Arg Ala Lys Glu Tyr Asn 
            20                  25                  30          
Lys Gln Phe Asn Ser Gln Leu Ala Ile Asp Lys Thr Gly Asn Met Gly 
        35                  40                  45              
Tyr Ile Ser Ile Pro Lys Ile Asn Ile Lys Leu Pro Leu Tyr His Gly 
    50                  55                  60                  
Thr Ser Glu Lys Val Leu Gln Thr Ser Ile Gly His Leu Glu Gly Ser 
65                  70                  75                  80  
Ser Leu Pro Ile Gly Gly Asp Ser Thr His Ser Ile Leu Ser Gly His 
                85                  90                  95      
Arg Gly Leu Pro Ser Ser Arg Leu Phe Ser Asp Leu Asp Lys Leu Lys 
            100                 105                 110         
Val Gly Asp His Trp Thr Val Ser Ile Leu Asn Glu Thr Tyr Thr Tyr 
        115                 120                 125             
Gln Val Asp Gln Ile Arg Thr Val Lys Pro Asp Asp Leu Arg Asp Leu 
    130                 135                 140                 
Gln Ile Val Lys Gly Lys Asp Tyr Gln Thr Leu Val Thr Cys Thr Pro 
145                 150                 155                 160 
Tyr Gly Val Asn Thr His Arg Leu Leu Val Arg Gly His Arg Val Pro 
                165                 170                 175     
Asn Asp Asn Gly Asn Ala Leu Val Val Ala Glu Ala Ile Gln Ile Glu 
            180                 185                 190         
Pro Ile Tyr Ile Ala Pro Phe Ile Ala Ile Phe Leu Thr Leu Ile Leu 
        195                 200                 205             
Leu Leu Ile Ser Leu Glu Val Thr Arg Arg Ala Arg Gln Arg Lys Lys 
    210                 215                 220                 
Ile Leu Lys Gln Ala Met Arg Lys Glu Glu Asn Asn Asp Leu 
225                 230                 235             

<210> 15
<211> 244
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C2 deletion mutant

<400> 15
Glu Ser Asn Gln Gln Ile Ala Asp Phe Asp Lys Glu Lys Ala Thr Leu 
1               5                   10                  15      
Asp Glu Ala Asp Ile Asp Glu Arg Met Lys Leu Ala Gln Ala Phe Asn 
            20                  25                  30          
Asp Ser Leu Glu Tyr Ala Arg Met Leu Glu Ile His Glu Arg Met Gly 
        35                  40                  45              
His Val Glu Ile Pro Val Ile Asp Val Asp Leu Pro Val Tyr Ala Gly 
    50                  55                  60                  
Thr Ala Glu Glu Val Leu Gln Gln Gly Ala Gly His Leu Glu Gly Thr 
65                  70                  75                  80  
Ser Leu Pro Ile Gly Gly Asn Ser Thr His Ala Val Ile Thr Ala His 
                85                  90                  95      
Thr Gly Leu Pro Thr Ala Lys Met Phe Thr Asp Leu Thr Lys Leu Lys 
            100                 105                 110         
Val Gly Asp Lys Phe Tyr Val His Asn Ile Lys Glu Val Met Ala Tyr 
        115                 120                 125             
Gln Val Asp Gln Val Lys Val Ile Glu Pro Thr Asn Phe Asp Asp Leu 
    130                 135                 140                 
Leu Ile Val Pro Gly His Asp Tyr Val Thr Leu Leu Thr Cys Thr Pro 
145                 150                 155                 160 
Tyr Met Ile Asn Thr His Arg Leu Leu Val Arg Gly His Arg Ile Pro 
                165                 170                 175     
Tyr Val Ala Glu Val Glu Glu Glu Phe Ile Ala Ala Asn Lys Leu Ser 
            180                 185                 190         
His Leu Tyr Arg Tyr Leu Phe Tyr Val Ala Val Gly Leu Ile Val Ile 
        195                 200                 205             
Leu Leu Trp Ile Ile Arg Arg Leu Arg Lys Lys Lys Lys Gln Pro Glu 
    210                 215                 220                 
Lys Ala Leu Lys Ala Leu Lys Ala Ala Arg Lys Glu Val Lys Val Glu 
225                 230                 235                 240 
Asp Gly Gln Gln 
                

<210> 16
<211> 235
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C2 deletion mutant

<400> 16
Glu Ser Asn Glu Val Ile Lys Glu Phe Asp Glu Thr Val Ser Gln Met 
1               5                   10                  15      
Asp Lys Ala Glu Leu Glu Glu Arg Trp Arg Leu Ala Gln Ala Phe Asn 
            20                  25                  30          
Ala Thr Leu Lys Glu Tyr Ala Asn Met Leu Lys Val His Glu Arg Ile 
        35                  40                  45              
Gly Tyr Val Glu Ile Pro Ala Ile Asp Gln Glu Ile Pro Met Tyr Val 
    50                  55                  60                  
Gly Thr Ser Glu Asp Ile Leu Gln Lys Gly Ala Gly Leu Leu Glu Gly 
65                  70                  75                  80  
Ala Ser Leu Pro Val Gly Gly Glu Asn Thr His Thr Val Ile Thr Ala 
                85                  90                  95      
His Arg Gly Leu Pro Thr Ala Glu Leu Phe Ser Gln Leu Asp Lys Met 
            100                 105                 110         
Lys Lys Gly Asp Ile Phe Tyr Leu His Val Leu Asp Gln Val Leu Ala 
        115                 120                 125             
Tyr Gln Val Asp Gln Ile Val Thr Val Glu Pro Asn Asp Phe Glu Pro 
    130                 135                 140                 
Val Leu Ile Gln His Gly Glu Asp Tyr Ala Thr Leu Leu Thr Cys Thr 
145                 150                 155                 160 
Pro Tyr Met Ile Asn Ser His Arg Leu Leu Val Arg Gly Lys Arg Ile 
                165                 170                 175     
Pro Tyr Thr Ala Pro Ile Ala Glu Arg Asn Arg Ala Val Arg Glu Arg 
            180                 185                 190         
Gly Gln Phe Trp Leu Trp Leu Leu Leu Gly Ala Met Ala Val Ile Leu 
        195                 200                 205             
Leu Leu Leu Tyr Arg Val Tyr Arg Asn Arg Arg Ile Val Lys Gly Leu 
    210                 215                 220                 
Glu Lys Gln Leu Glu Gly Arg His Val Lys Asp 
225                 230                 235 

<210> 17
<211> 240
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C3 deletion mutant

<400> 17
Gln Ser Leu Gly Gln Val Lys Gly His Ala Thr Phe Val Lys Ser Met 
1               5                   10                  15      
Thr Thr Glu Met Tyr Gln Glu Gln Gln Asn His Ser Leu Ala Tyr Asn 
            20                  25                  30          
Gln Arg Leu Ala Ser Gln Glu Val Asn Tyr Gln Val Ser Asp Asp Pro 
        35                  40                  45              
Asp Ala Val Tyr Gly Tyr Leu Ser Ile Pro Ser Leu Glu Ile Met Glu 
    50                  55                  60                  
Pro Val Tyr Leu Gly Ala Asp Tyr His His Leu Gly Met Gly Leu Ala 
65                  70                  75                  80  
His Val Asp Gly Thr Pro Leu Pro Leu Asp Gly Thr Gly Ile Arg Ser 
                85                  90                  95      
Val Ile Ala Gly His Arg Ala Glu Pro Ser His Val Phe Phe Arg His 
            100                 105                 110         
Leu Asp Gln Leu Lys Val Gly Asp Ala Leu Tyr Tyr Asp Asn Gly Gln 
        115                 120                 125             
Glu Ile Val Glu Tyr Gln Met Met Asp Thr Glu Ile Ile Leu Pro Ser 
    130                 135                 140                 
Glu Trp Glu Lys Leu Glu Ser Val Ser Ser Lys Asn Ile Met Thr Leu 
145                 150                 155                 160 
Ile Thr Cys Asp Pro Ile Pro Thr Phe Asn Lys Arg Leu Leu Val Asn 
                165                 170                 175     
Phe Glu Arg Val Ala Val Tyr Gln Lys Ser Asp Pro Gln Thr Ala Ala 
            180                 185                 190         
Val Ala Arg Val Ala Phe Thr Lys Glu Gly Gln Ser Val Ser Arg Val 
        195                 200                 205             
Ala Thr Ser Gln Trp Leu Tyr Arg Gly Leu Val Val Leu Ala Phe Leu 
    210                 215                 220                 
Gly Ile Leu Phe Val Leu Trp Lys Leu Ala Arg Leu Leu Arg Gly Lys 
225                 230                 235                 240 



<210> 18
<211> 229
<212> PRT
<213> Streptococcus pyogenes

<220> 
<223> GAS Sortase C1 deletion mutant

<400> 18
Arg Asp Arg Gln Leu Leu Ser Thr Tyr His Lys Gln Val Thr Gln Lys 
1               5                   10                  15      
Lys Pro Ser Glu Met Glu Glu Val Trp Gln Lys Ala Lys Ala Tyr Asn 
            20                  25                  30          
Ala Arg Leu Asn Tyr Glu Ser Leu Leu Gln Ile Glu Asn Asn Asp Ile 
        35                  40                  45              
Met Gly Tyr Val Glu Val Pro Ser Ile Lys Val Thr Leu Pro Ile Tyr 
    50                  55                  60                  
His Tyr Thr Thr Asp Glu Val Leu Thr Lys Gly Ala Gly His Leu Phe 
65                  70                  75                  80  
Gly Ser Ala Leu Pro Val Gly Gly Asp Gly Thr His Thr Val Ile Ser 
                85                  90                  95      
Ala His Arg Gly Leu Pro Ser Ala Glu Met Phe Thr Asn Leu Asn Leu 
            100                 105                 110         
Val Lys Lys Gly Asp Thr Phe Tyr Phe Arg Val Leu Asn Lys Val Leu 
        115                 120                 125             
Ala Tyr Lys Val Asp Gln Ile Leu Thr Val Glu Pro Asp Gln Val Thr 
    130                 135                 140                 
Ser Leu Ser Gly Val Met Gly Lys Asp Tyr Ala Thr Leu Val Thr Cys 
145                 150                 155                 160 
Thr Pro Tyr Gly Val Asn Thr Lys Arg Leu Leu Val Arg Gly His Arg 
                165                 170                 175     
Ile Ala Tyr His Tyr Lys Lys Tyr Gln Gln Ala Lys Lys Ala Met Lys 
            180                 185                 190         
Leu Val Asp Lys Ser Arg Met Trp Ala Glu Val Val Cys Ala Ala Phe 
        195                 200                 205             
Gly Val Val Ile Ala Ile Ile Leu Val Phe Met Tyr Ser Arg Val Ser 
    210                 215                 220                 
Ala Lys Lys Ser Lys 
225                 

<210> 19
<211> 203
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS Sortase C1 of PI-1 deletion mutant

<400> 19
Glu Tyr Ala Arg Met Leu Glu Val Lys Glu Gln Ile Gly His Val Ile 
1               5                   10                  15      
Ile Pro Arg Ile Asn Gln Asp Ile Pro Ile Tyr Ala Gly Ser Ala Glu 
            20                  25                  30          
Glu Asn Leu Gln Arg Gly Val Gly His Leu Glu Gly Thr Ser Leu Pro 
        35                  40                  45              
Val Gly Gly Glu Ser Thr His Ala Val Leu Thr Ala His Arg Gly Leu 
    50                  55                  60                  
Pro Thr Ala Lys Leu Phe Thr Asn Leu Asp Lys Val Thr Val Gly Asp 
65                  70                  75                  80  
Arg Phe Tyr Ile Glu His Ile Gly Gly Lys Ile Ala Tyr Gln Val Asp 
                85                  90                  95      
Gln Ile Lys Val Ile Ala Pro Asp Gln Leu Glu Asp Leu Tyr Val Ile 
            100                 105                 110         
Gln Gly Glu Asp His Val Thr Leu Leu Thr Cys Thr Pro Tyr Met Ile 
        115                 120                 125             
Asn Ser His Arg Leu Leu Val Arg Gly Lys Arg Ile Pro Tyr Val Glu 
    130                 135                 140                 
Lys Thr Val Gln Lys Asp Ser Lys Thr Phe Arg Gln Gln Gln Tyr Leu 
145                 150                 155                 160 
Thr Tyr Ala Met Trp Val Val Val Gly Leu Ile Leu Leu Ser Leu Leu 
                165                 170                 175     
Ile Trp Phe Lys Lys Thr Lys Gln Lys Lys Arg Arg Lys Asn Glu Lys 
            180                 185                 190         
Ala Ala Ser Gln Asn Ser His Asn Asn Ser Lys 
        195                 200             

<210> 20
<211> 188
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS sortase C2 of PI-1 deletion mutant

<400> 20
Glu Tyr Ala Arg Met Leu Glu Val His Glu Gln Ile Gly His Val Ala 
1               5                   10                  15      
Ile Pro Ser Ile Gly Val Asp Ile Pro Ile Tyr Ala Gly Thr Ser Glu 
            20                  25                  30          
Thr Val Leu Gln Lys Gly Ser Gly His Leu Glu Gly Thr Ser Leu Pro 
        35                  40                  45              
Val Gly Gly Leu Ser Thr His Ser Val Leu Thr Ala His Arg Gly Leu 
    50                  55                  60                  
Pro Thr Ala Arg Leu Phe Thr Asp Leu Asn Lys Val Lys Lys Gly Gln 
65                  70                  75                  80  
Ile Phe Tyr Val Thr Asn Ile Lys Glu Thr Leu Ala Tyr Lys Val Val 
                85                  90                  95      
Ser Ile Lys Val Val Asp Pro Thr Ala Leu Ser Glu Val Lys Ile Val 
            100                 105                 110         
Asn Gly Lys Asp Tyr Ile Thr Leu Leu Thr Cys Thr Pro Tyr Met Ile 
        115                 120                 125             
Asn Ser His Arg Leu Leu Val Lys Gly Glu Arg Ile Pro Tyr Asp Ser 
    130                 135                 140                 
Thr Glu Ala Glu Lys His Lys Glu Gln Thr Val Gln Asp Tyr Arg Leu 
145                 150                 155                 160 
Ser Leu Val Leu Lys Ile Leu Leu Val Leu Leu Ile Gly Leu Phe Ile 
                165                 170                 175     
Val Ile Met Met Arg Arg Trp Met Gln His Arg Gln 
            180                 185             

<210> 21
<211> 196
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS sortase C1 of PI-2a deletion mutant

<400> 21
Glu Tyr Ala Arg Met Leu Glu Val Ser Glu Lys Ile Gly Ile Ile Ser 
1               5                   10                  15      
Val Pro Lys Ile Gly Gln Lys Leu Pro Ile Phe Ala Gly Ser Ser Gln 
            20                  25                  30          
Glu Val Leu Ser Lys Gly Ala Gly His Leu Glu Gly Thr Ser Leu Pro 
        35                  40                  45              
Ile Gly Gly Asn Ser Thr His Thr Val Ile Thr Ala His Ser Gly Ile 
    50                  55                  60                  
Pro Asp Lys Glu Leu Phe Ser Asn Leu Lys Lys Leu Lys Lys Gly Asp 
65                  70                  75                  80  
Lys Phe Tyr Ile Gln Asn Ile Lys Glu Thr Ile Ala Tyr Gln Val Asp 
                85                  90                  95      
Gln Ile Lys Val Val Thr Pro Asp Asn Phe Ser Asp Leu Leu Val Val 
            100                 105                 110         
Pro Gly His Asp Tyr Ala Thr Leu Leu Thr Cys Thr Pro Ile Met Val 
        115                 120                 125             
Asn Thr His Arg Leu Leu Val Arg Gly His Arg Ile Pro Tyr Lys Gly 
    130                 135                 140                 
Pro Ile Asp Glu Lys Leu Ile Lys Asp Gly His Leu Asn Thr Ile Tyr 
145                 150                 155                 160 
Arg Tyr Leu Phe Tyr Ile Ser Leu Val Ile Ile Ala Trp Leu Leu Trp 
                165                 170                 175     
Leu Ile Lys Arg Gln Arg Gln Lys Asn Arg Leu Ser Ser Val Arg Lys 
            180                 185                 190         
Gly Ile Glu Ser 
        195     

<210> 22
<211> 195
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS sortase C2 of PI-2a deletion mutant

<400> 22
Glu Tyr Ala His Met Leu Glu Ile Thr Glu Met Ile Gly Tyr Ile Asp 
1               5                   10                  15      
Ile Pro Ser Ile Lys Gln Lys Leu Pro Ile Tyr Ala Gly Thr Thr Ser 
            20                  25                  30          
Ser Val Leu Glu Lys Gly Ser Gly His Leu Glu Gly Thr Ser Leu Pro 
        35                  40                  45              
Ile Gly Gly Lys Ser Ser His Thr Val Ile Thr Ala His Arg Gly Leu 
    50                  55                  60                  
Pro Lys Ala Lys Leu Phe Thr Asp Leu Asp Lys Leu Lys Lys Gly Lys 
65                  70                  75                  80  
Ile Phe Tyr Ile His Asn Ile Lys Glu Val Leu Ala Tyr Lys Val Asp 
                85                  90                  95      
Gln Ile Ser Val Val Lys Pro Asp Asn Phe Ser Lys Leu Leu Val Val 
            100                 105                 110         
Lys Gly Lys Asp Tyr Ala Thr Leu Leu Thr Cys Thr Pro Tyr Ser Ile 
        115                 120                 125             
Asn Ser His Arg Leu Leu Val Arg Gly His Arg Ile Lys Tyr Val Pro 
    130                 135                 140                 
Pro Val Lys Glu Lys Asn Tyr Leu Met Lys Glu Leu Gln Thr His Tyr 
145                 150                 155                 160 
Lys Leu Tyr Phe Leu Leu Ser Ile Leu Val Ile Leu Ile Leu Val Ala 
                165                 170                 175     
Leu Leu Leu Tyr Leu Lys Arg Lys Phe Lys Glu Arg Lys Arg Lys Gly 
            180                 185                 190         
Asn Gln Lys 
        195 

<210> 23
<211> 203
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS sortase C1 of PI-2b deletion mutant

<400> 23
Asn Ser Gln Leu Ala Ile Asp Lys Thr Gly Asn Met Gly Tyr Ile Ser 
1               5                   10                  15      
Ile Pro Lys Ile Asn Ile Lys Leu Pro Leu Tyr His Gly Thr Ser Glu 
            20                  25                  30          
Lys Val Leu Gln Thr Ser Ile Gly His Leu Glu Gly Ser Ser Leu Pro 
        35                  40                  45              
Ile Gly Gly Asp Ser Thr His Ser Ile Leu Ser Gly His Arg Gly Leu 
    50                  55                  60                  
Pro Ser Ser Arg Leu Phe Ser Asp Leu Asp Lys Leu Lys Val Gly Asp 
65                  70                  75                  80  
His Trp Thr Val Ser Ile Leu Asn Glu Thr Tyr Thr Tyr Gln Val Asp 
                85                  90                  95      
Gln Ile Arg Thr Val Lys Pro Asp Asp Leu Arg Asp Leu Gln Ile Val 
            100                 105                 110         
Lys Gly Lys Asp Tyr Gln Thr Leu Val Thr Cys Thr Pro Tyr Gly Val 
        115                 120                 125             
Asn Thr His Arg Leu Leu Val Arg Gly His Arg Val Pro Asn Asp Asn 
    130                 135                 140                 
Gly Asn Ala Leu Val Val Ala Glu Ala Ile Gln Ile Glu Pro Ile Tyr 
145                 150                 155                 160 
Ile Ala Pro Phe Ile Ala Ile Phe Leu Thr Leu Ile Leu Leu Leu Ile 
                165                 170                 175     
Ser Leu Glu Val Thr Arg Arg Ala Arg Gln Arg Lys Lys Ile Leu Lys 
            180                 185                 190         
Gln Ala Met Arg Lys Glu Glu Asn Asn Asp Leu 
        195                 200             

<210> 24
<211> 209
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C1 deletion mutant

<400> 24
Glu Tyr Ala Arg Met Leu Glu Ile His Glu Arg Met Gly His Val Glu 
1               5                   10                  15      
Ile Pro Val Ile Asp Val Asp Leu Pro Val Tyr Ala Gly Thr Ala Glu 
            20                  25                  30          
Glu Val Leu Gln Gln Gly Ala Gly His Leu Glu Gly Thr Ser Leu Pro 
        35                  40                  45              
Ile Gly Gly Asn Ser Thr His Ala Val Ile Thr Ala His Thr Gly Leu 
    50                  55                  60                  
Pro Thr Ala Lys Met Phe Thr Asp Leu Thr Lys Leu Lys Val Gly Asp 
65                  70                  75                  80  
Lys Phe Tyr Val His Asn Ile Lys Glu Val Met Ala Tyr Gln Val Asp 
                85                  90                  95      
Gln Val Lys Val Ile Glu Pro Thr Asn Phe Asp Asp Leu Leu Ile Val 
            100                 105                 110         
Pro Gly His Asp Tyr Val Thr Leu Leu Thr Cys Thr Pro Tyr Met Ile 
        115                 120                 125             
Asn Thr His Arg Leu Leu Val Arg Gly His Arg Ile Pro Tyr Val Ala 
    130                 135                 140                 
Glu Val Glu Glu Glu Phe Ile Ala Ala Asn Lys Leu Ser His Leu Tyr 
145                 150                 155                 160 
Arg Tyr Leu Phe Tyr Val Ala Val Gly Leu Ile Val Ile Leu Leu Trp 
                165                 170                 175     
Ile Ile Arg Arg Leu Arg Lys Lys Lys Lys Gln Pro Glu Lys Ala Leu 
            180                 185                 190         
Lys Ala Leu Lys Ala Ala Arg Lys Glu Val Lys Val Glu Asp Gly Gln 
        195                 200                 205             
Gln 
    

<210> 25
<211> 199
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C2 deletion mutant

<400> 25
Glu Tyr Ala Asn Met Leu Lys Val His Glu Arg Ile Gly Tyr Val Glu 
1               5                   10                  15      
Ile Pro Ala Ile Asp Gln Glu Ile Pro Met Tyr Val Gly Thr Ser Glu 
            20                  25                  30          
Asp Ile Leu Gln Lys Gly Ala Gly Leu Leu Glu Gly Ala Ser Leu Pro 
        35                  40                  45              
Val Gly Gly Glu Asn Thr His Thr Val Ile Thr Ala His Arg Gly Leu 
    50                  55                  60                  
Pro Thr Ala Glu Leu Phe Ser Gln Leu Asp Lys Met Lys Lys Gly Asp 
65                  70                  75                  80  
Ile Phe Tyr Leu His Val Leu Asp Gln Val Leu Ala Tyr Gln Val Asp 
                85                  90                  95      
Gln Ile Val Thr Val Glu Pro Asn Asp Phe Glu Pro Val Leu Ile Gln 
            100                 105                 110         
His Gly Glu Asp Tyr Ala Thr Leu Leu Thr Cys Thr Pro Tyr Met Ile 
        115                 120                 125             
Asn Ser His Arg Leu Leu Val Arg Gly Lys Arg Ile Pro Tyr Thr Ala 
    130                 135                 140                 
Pro Ile Ala Glu Arg Asn Arg Ala Val Arg Glu Arg Gly Gln Phe Trp 
145                 150                 155                 160 
Leu Trp Leu Leu Leu Gly Ala Met Ala Val Ile Leu Leu Leu Leu Tyr 
                165                 170                 175     
Arg Val Tyr Arg Asn Arg Arg Ile Val Lys Gly Leu Glu Lys Gln Leu 
            180                 185                 190         
Glu Gly Arg His Val Lys Asp 
        195                 

<210> 26
<211> 202
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C3 deletion mutant

<400> 26
Glu Val Asn Tyr Gln Val Ser Asp Asp Pro Asp Ala Val Tyr Gly Tyr 
1               5                   10                  15      
Leu Ser Ile Pro Ser Leu Glu Ile Met Glu Pro Val Tyr Leu Gly Ala 
            20                  25                  30          
Asp Tyr His His Leu Gly Met Gly Leu Ala His Val Asp Gly Thr Pro 
        35                  40                  45              
Leu Pro Leu Asp Gly Thr Gly Ile Arg Ser Val Ile Ala Gly His Arg 
    50                  55                  60                  
Ala Glu Pro Ser His Val Phe Phe Arg His Leu Asp Gln Leu Lys Val 
65                  70                  75                  80  
Gly Asp Ala Leu Tyr Tyr Asp Asn Gly Gln Glu Ile Val Glu Tyr Gln 
                85                  90                  95      
Met Met Asp Thr Glu Ile Ile Leu Pro Ser Glu Trp Glu Lys Leu Glu 
            100                 105                 110         
Ser Val Ser Ser Lys Asn Ile Met Thr Leu Ile Thr Cys Asp Pro Ile 
        115                 120                 125             
Pro Thr Phe Asn Lys Arg Leu Leu Val Asn Phe Glu Arg Val Ala Val 
    130                 135                 140                 
Tyr Gln Lys Ser Asp Pro Gln Thr Ala Ala Val Ala Arg Val Ala Phe 
145                 150                 155                 160 
Thr Lys Glu Gly Gln Ser Val Ser Arg Val Ala Thr Ser Gln Trp Leu 
                165                 170                 175     
Tyr Arg Gly Leu Val Val Leu Ala Phe Leu Gly Ile Leu Phe Val Leu 
            180                 185                 190         
Trp Lys Leu Ala Arg Leu Leu Arg Gly Lys 
        195                 200         

<210> 27
<211> 197
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GAS sortase C1 deletion mutant

<400> 27
His Asp Lys Asn Tyr Glu Ser Leu Leu Gln Ile Glu Asn Asn Asp Ile 
1               5                   10                  15      
Met Gly Tyr Val Glu Val Pro Ser Ile Lys Val Thr Leu Pro Ile Tyr 
            20                  25                  30          
His Tyr Thr Thr Asp Glu Val Leu Thr Lys Gly Ala Gly His Leu Phe 
        35                  40                  45              
Gly Ser Ala Leu Pro Val Gly Gly Asp Gly Thr His Thr Val Ile Ser 
    50                  55                  60                  
Ala His Arg Gly Leu Pro Ser Ala Glu Met Phe Thr Asn Leu Asn Leu 
65                  70                  75                  80  
Val Lys Lys Gly Asp Thr Phe Tyr Phe Arg Val Leu Asn Lys Val Leu 
                85                  90                  95      
Ala Tyr Lys Val Asp Gln Ile Leu Thr Val Glu Pro Asp Gln Val Thr 
            100                 105                 110         
Ser Leu Ser Gly Val Met Gly Lys Asp Tyr Ala Thr Leu Val Thr Cys 
        115                 120                 125             
Thr Pro Tyr Gly Val Asn Thr Lys Arg Leu Leu Val Arg Gly His Arg 
    130                 135                 140                 
Ile Ala Tyr His Tyr Lys Lys Tyr Gln Gln Ala Lys Lys Ala Met Lys 
145                 150                 155                 160 
Leu Val Asp Lys Ser Arg Met Trp Ala Glu Val Val Cys Ala Ala Phe 
                165                 170                 175     
Gly Val Val Ile Ala Ile Ile Leu Val Phe Met Tyr Ser Arg Val Ser 
            180                 185                 190         
Ala Lys Lys Ser Lys 
        195         

<210> 28
<211> 270
<212> PRT
<213> Streptococcus pyogenes

<220> 
<223> GBS sortase C1 of PI-1 deletion mutant

<400> 28
Ile Val Ser Gln Val Met Tyr Phe Gln Ala Ser His Ala Asn Ile Asn 
1               5                   10                  15      
Ala Phe Lys Glu Ala Val Thr Lys Ile Asp Arg Val Glu Ile Asn Arg 
            20                  25                  30          
Arg Leu Glu Leu Ala Tyr Ala Tyr Asn Ala Ser Ile Ala Gly Ala Lys 
        35                  40                  45              
Thr Asn Gly Glu Tyr Pro Ala Leu Lys Ser Ala Glu Gln Lys Gln Ala 
    50                  55                  60                  
Gly Val Val Glu Tyr Ala Arg Met Leu Glu Val Lys Glu Gln Ile Gly 
65                  70                  75                  80  
His Val Ile Ile Pro Arg Ile Asn Gln Asp Ile Pro Ile Tyr Ala Gly 
                85                  90                  95      
Ser Ala Glu Glu Asn Leu Gln Arg Gly Val Gly His Leu Glu Gly Thr 
            100                 105                 110         
Ser Leu Pro Val Gly Gly Glu Ser Thr His Ala Val Leu Thr Ala His 
        115                 120                 125             
Arg Gly Leu Pro Thr Ala Lys Leu Phe Thr Asn Leu Asp Lys Val Thr 
    130                 135                 140                 
Val Gly Asp Arg Phe Tyr Ile Glu His Ile Gly Gly Lys Ile Ala Tyr 
145                 150                 155                 160 
Gln Val Asp Gln Ile Lys Val Ile Ala Pro Asp Gln Leu Glu Asp Leu 
                165                 170                 175     
Tyr Val Ile Gln Gly Glu Asp His Val Thr Leu Leu Thr Cys Thr Pro 
            180                 185                 190         
Tyr Met Ile Asn Ser His Arg Leu Leu Val Arg Gly Lys Arg Ile Pro 
        195                 200                 205             
Tyr Val Glu Lys Thr Val Gln Lys Asp Ser Lys Thr Phe Arg Gln Gln 
    210                 215                 220                 
Gln Tyr Leu Thr Tyr Ala Met Trp Val Val Val Gly Leu Ile Leu Leu 
225                 230                 235                 240 
Ser Leu Leu Ile Trp Phe Lys Lys Thr Lys Gln Lys Lys Arg Arg Lys 
                245                 250                 255     
Asn Glu Lys Ala Ala Ser Gln Asn Ser His Asn Asn Ser Lys 
            260                 265                 270 

<210> 29
<211> 239
<212> PRT
<213> Streptococcus pyogenes

<220> 
<223> GBS sortase C2 of PI-1

<400> 29
Ala Ser His Gln Asn Ile Asn Gln Phe Lys Arg Glu Val Ala Lys Ile 
1               5                   10                  15      
Asp Thr Asn Thr Val Glu Arg Arg Ile Ala Leu Ala Asn Ala Tyr Asn 
            20                  25                  30          
Glu Thr Leu Ser Arg Asn Pro Leu Leu Ile Thr Ser Lys Gln Lys Glu 
        35                  40                  45              
Gly Leu Arg Glu Tyr Ala Arg Met Leu Glu Val His Glu Gln Ile Gly 
    50                  55                  60                  
His Val Ala Ile Pro Ser Ile Gly Val Asp Ile Pro Ile Tyr Ala Gly 
65                  70                  75                  80  
Thr Ser Glu Thr Val Leu Gln Lys Gly Ser Gly His Leu Glu Gly Thr 
                85                  90                  95      
Ser Leu Pro Val Gly Gly Leu Ser Thr His Ser Val Leu Thr Ala His 
            100                 105                 110         
Arg Gly Leu Pro Thr Ala Arg Leu Phe Thr Asp Leu Asn Lys Val Lys 
        115                 120                 125             
Lys Gly Gln Ile Phe Tyr Val Thr Asn Ile Lys Glu Thr Leu Ala Tyr 
    130                 135                 140                 
Lys Val Val Ser Ile Lys Val Val Asp Pro Thr Ala Leu Ser Glu Val 
145                 150                 155                 160 
Lys Ile Val Asn Gly Lys Asp Tyr Ile Thr Leu Leu Thr Cys Thr Pro 
                165                 170                 175     
Tyr Met Ile Asn Ser His Arg Leu Leu Val Lys Gly Glu Arg Ile Pro 
            180                 185                 190         
Tyr Asp Ser Thr Glu Ala Glu Lys His Lys Glu Gln Thr Val Gln Asp 
        195                 200                 205             
Tyr Arg Leu Ser Leu Val Leu Lys Ile Leu Leu Val Leu Leu Ile Gly 
    210                 215                 220                 
Leu Phe Ile Val Ile Met Met Arg Arg Trp Met Gln His Arg Gln 
225                 230                 235                 

<210> 30
<211> 247
<212> PRT
<213> Streptococcus pyogenes

<220> 
<223> GBS sortase C1 of PI-2a deletion mutant

<400> 30
Glu Ser Asn Asn Gln Thr Gln Asp Phe Glu Arg Ala Ala Lys Lys Leu 
1               5                   10                  15      
Ser Gln Lys Glu Ile Asn Arg Arg Met Ala Leu Ala Gln Ala Tyr Asn 
            20                  25                  30          
Asp Ser Leu Asn Asn Val His Leu Glu Glu Lys Lys Arg Ile Gln Lys 
        35                  40                  45              
Gly Ile Ala Glu Tyr Ala Arg Met Leu Glu Val Ser Glu Lys Ile Gly 
    50                  55                  60                  
Ile Ile Ser Val Pro Lys Ile Gly Gln Lys Leu Pro Ile Phe Ala Gly 
65                  70                  75                  80  
Ser Ser Gln Glu Val Leu Ser Lys Gly Ala Gly His Leu Glu Gly Thr 
                85                  90                  95      
Ser Leu Pro Ile Gly Gly Asn Ser Thr His Thr Val Ile Thr Ala His 
            100                 105                 110         
Ser Gly Ile Pro Asp Lys Glu Leu Phe Ser Asn Leu Lys Lys Leu Lys 
        115                 120                 125             
Lys Gly Asp Lys Phe Tyr Ile Gln Asn Ile Lys Glu Thr Ile Ala Tyr 
    130                 135                 140                 
Gln Val Asp Gln Ile Lys Val Val Thr Pro Asp Asn Phe Ser Asp Leu 
145                 150                 155                 160 
Leu Val Val Pro Gly His Asp Tyr Ala Thr Leu Leu Thr Cys Thr Pro 
                165                 170                 175     
Ile Met Val Asn Thr His Arg Leu Leu Val Arg Gly His Arg Ile Pro 
            180                 185                 190         
Tyr Lys Gly Pro Ile Asp Glu Lys Leu Ile Lys Asp Gly His Leu Asn 
        195                 200                 205             
Thr Ile Tyr Arg Tyr Leu Phe Tyr Ile Ser Leu Val Ile Ile Ala Trp 
    210                 215                 220                 
Leu Leu Trp Leu Ile Lys Arg Gln Arg Gln Lys Asn Arg Leu Ser Ser 
225                 230                 235                 240 
Val Arg Lys Gly Ile Glu Ser 
                245         

<210> 31
<211> 245
<212> PRT
<213> Streptococcus pyogenes

<220> 
<223> GBS sortase C2 of PI-2a deletion mutant

<400> 31
Lys Ala Asn Asn Gln Val Thr Asn Phe Asp Asn Gln Thr Gln Lys Leu 
1               5                   10                  15      
Asn Ala Lys Glu Ile Asn Arg Arg Phe Glu Leu Ala Lys Ala Tyr Asn 
            20                  25                  30          
Arg Thr Leu Asp Pro Ser Arg Leu Ser Thr Glu Lys Glu Lys Lys Gly 
        35                  40                  45              
Ile Ala Glu Tyr Ala His Met Leu Glu Ile Thr Glu Met Ile Gly Tyr 
    50                  55                  60                  
Ile Asp Ile Pro Ser Ile Lys Gln Lys Leu Pro Ile Tyr Ala Gly Thr 
65                  70                  75                  80  
Thr Ser Ser Val Leu Glu Lys Gly Ser Gly His Leu Glu Gly Thr Ser 
                85                  90                  95      
Leu Pro Ile Gly Gly Lys Ser Ser His Thr Val Ile Thr Ala His Arg 
            100                 105                 110         
Gly Leu Pro Lys Ala Lys Leu Phe Thr Asp Leu Asp Lys Leu Lys Lys 
        115                 120                 125             
Gly Lys Ile Phe Tyr Ile His Asn Ile Lys Glu Val Leu Ala Tyr Lys 
    130                 135                 140                 
Val Asp Gln Ile Ser Val Val Lys Pro Asp Asn Phe Ser Lys Leu Leu 
145                 150                 155                 160 
Val Val Lys Gly Lys Asp Tyr Ala Thr Leu Leu Thr Cys Thr Pro Tyr 
                165                 170                 175     
Ser Ile Asn Ser His Arg Leu Leu Val Arg Gly His Arg Ile Lys Tyr 
            180                 185                 190         
Val Pro Pro Val Lys Glu Lys Asn Tyr Leu Met Lys Glu Leu Gln Thr 
        195                 200                 205             
His Tyr Lys Leu Tyr Phe Leu Leu Ser Ile Leu Val Ile Leu Ile Leu 
    210                 215                 220                 
Val Ala Leu Leu Leu Tyr Leu Lys Arg Lys Phe Lys Glu Arg Lys Arg 
225                 230                 235                 240 
Lys Gly Asn Gln Lys 
                245 

<210> 32
<211> 252
<212> PRT
<213> Streptococcus pyogenes

<220> 
<223> GBS sortase C1 of PI-2b deletion mutant

<400> 32
His Gln Ser Arg Ala Ile Met Asp Tyr Gln Asp Arg Val Thr His Met 
1               5                   10                  15      
Asp Glu Asn Asp Tyr Lys Lys Ile Ile Asn Arg Ala Lys Glu Tyr Asn 
            20                  25                  30          
Lys Gln Phe Lys Thr Ser Gly His Met Thr Ser Gln Glu Arg Leu Asp 
        35                  40                  45              
Tyr Asn Ser Gln Leu Ala Ile Asp Lys Thr Gly Asn Met Gly Tyr Ile 
    50                  55                  60                  
Ser Ile Pro Lys Ile Asn Ile Lys Leu Pro Leu Tyr His Gly Thr Ser 
65                  70                  75                  80  
Glu Lys Val Leu Gln Thr Ser Ile Gly His Leu Glu Gly Ser Ser Leu 
                85                  90                  95      
Pro Ile Gly Gly Asp Ser Thr His Ser Ile Leu Ser Gly His Arg Gly 
            100                 105                 110         
Leu Pro Ser Ser Arg Leu Phe Ser Asp Leu Asp Lys Leu Lys Val Gly 
        115                 120                 125             
Asp His Trp Thr Val Ser Ile Leu Asn Glu Thr Tyr Thr Tyr Gln Val 
    130                 135                 140                 
Asp Gln Ile Arg Thr Val Lys Pro Asp Asp Leu Arg Asp Leu Gln Ile 
145                 150                 155                 160 
Val Lys Gly Lys Asp Tyr Gln Thr Leu Val Thr Cys Thr Pro Tyr Gly 
                165                 170                 175     
Val Asn Thr His Arg Leu Leu Val Arg Gly His Arg Val Pro Asn Asp 
            180                 185                 190         
Asn Gly Asn Ala Leu Val Val Ala Glu Ala Ile Gln Ile Glu Pro Ile 
        195                 200                 205             
Tyr Ile Ala Pro Phe Ile Ala Ile Phe Leu Thr Leu Ile Leu Leu Leu 
    210                 215                 220                 
Ile Ser Leu Glu Val Thr Arg Arg Ala Arg Gln Arg Lys Lys Ile Leu 
225                 230                 235                 240 
Lys Gln Ala Met Arg Lys Glu Glu Asn Asn Asp Leu 
                245                 250         

<210> 33
<211> 260
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C1 deletion mutant

<400> 33
Glu Ser Asn Gln Gln Ile Ala Asp Phe Asp Lys Glu Lys Ala Thr Leu 
1               5                   10                  15      
Asp Glu Ala Asp Ile Asp Glu Arg Met Lys Leu Ala Gln Ala Phe Asn 
            20                  25                  30          
Asp Ser Leu Asn Asn Val Val Ser Gly Ser Glu Glu Met Lys Lys Lys 
        35                  40                  45              
Gly Arg Ala Glu Tyr Ala Arg Met Leu Glu Ile His Glu Arg Met Gly 
    50                  55                  60                  
His Val Glu Ile Pro Val Ile Asp Val Asp Leu Pro Val Tyr Ala Gly 
65                  70                  75                  80  
Thr Ala Glu Glu Val Leu Gln Gln Gly Ala Gly His Leu Glu Gly Thr 
                85                  90                  95      
Ser Leu Pro Ile Gly Gly Asn Ser Thr His Ala Val Ile Thr Ala His 
            100                 105                 110         
Thr Gly Leu Pro Thr Ala Lys Met Phe Thr Asp Leu Thr Lys Leu Lys 
        115                 120                 125             
Val Gly Asp Lys Phe Tyr Val His Asn Ile Lys Glu Val Met Ala Tyr 
    130                 135                 140                 
Gln Val Asp Gln Val Lys Val Ile Glu Pro Thr Asn Phe Asp Asp Leu 
145                 150                 155                 160 
Leu Ile Val Pro Gly His Asp Tyr Val Thr Leu Leu Thr Cys Thr Pro 
                165                 170                 175     
Tyr Met Ile Asn Thr His Arg Leu Leu Val Arg Gly His Arg Ile Pro 
            180                 185                 190         
Tyr Val Ala Glu Val Glu Glu Glu Phe Ile Ala Ala Asn Lys Leu Ser 
        195                 200                 205             
His Leu Tyr Arg Tyr Leu Phe Tyr Val Ala Val Gly Leu Ile Val Ile 
    210                 215                 220                 
Leu Leu Trp Ile Ile Arg Arg Leu Arg Lys Lys Lys Lys Gln Pro Glu 
225                 230                 235                 240 
Lys Ala Leu Lys Ala Leu Lys Ala Ala Arg Lys Glu Val Lys Val Glu 
                245                 250                 255     
Asp Gly Gln Gln 
            260 

<210> 34
<211> 250
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C2 deletion mutant

<400> 34
Glu Ser Asn Glu Val Ile Lys Glu Phe Asp Glu Thr Val Ser Gln Met 
1               5                   10                  15      
Asp Lys Ala Glu Leu Glu Glu Arg Trp Arg Leu Ala Gln Ala Phe Asn 
            20                  25                  30          
Ala Thr Leu Lys Pro Ser Glu Ile Leu Thr Glu Gln Glu Lys Lys Lys 
        35                  40                  45              
Gly Val Ser Glu Tyr Ala Asn Met Leu Lys Val His Glu Arg Ile Gly 
    50                  55                  60                  
Tyr Val Glu Ile Pro Ala Ile Asp Gln Glu Ile Pro Met Tyr Val Gly 
65                  70                  75                  80  
Thr Ser Glu Asp Ile Leu Gln Lys Gly Ala Gly Leu Leu Glu Gly Ala 
                85                  90                  95      
Ser Leu Pro Val Gly Gly Glu Asn Thr His Thr Val Ile Thr Ala His 
            100                 105                 110         
Arg Gly Leu Pro Thr Ala Glu Leu Phe Ser Gln Leu Asp Lys Met Lys 
        115                 120                 125             
Lys Gly Asp Ile Phe Tyr Leu His Val Leu Asp Gln Val Leu Ala Tyr 
    130                 135                 140                 
Gln Val Asp Gln Ile Val Thr Val Glu Pro Asn Asp Phe Glu Pro Val 
145                 150                 155                 160 
Leu Ile Gln His Gly Glu Asp Tyr Ala Thr Leu Leu Thr Cys Thr Pro 
                165                 170                 175     
Tyr Met Ile Asn Ser His Arg Leu Leu Val Arg Gly Lys Arg Ile Pro 
            180                 185                 190         
Tyr Thr Ala Pro Ile Ala Glu Arg Asn Arg Ala Val Arg Glu Arg Gly 
        195                 200                 205             
Gln Phe Trp Leu Trp Leu Leu Leu Gly Ala Met Ala Val Ile Leu Leu 
    210                 215                 220                 
Leu Leu Tyr Arg Val Tyr Arg Asn Arg Arg Ile Val Lys Gly Leu Glu 
225                 230                 235                 240 
Lys Gln Leu Glu Gly Arg His Val Lys Asp 
                245                 250 

<210> 35
<211> 249
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C3

<400> 35
Gln Ser Leu Gly Gln Val Lys Gly His Ala Thr Phe Val Lys Ser Met 
1               5                   10                  15      
Thr Thr Glu Met Tyr Gln Glu Gln Gln Asn His Ser Leu Ala Tyr Asn 
            20                  25                  30          
Gln Arg Leu Ala Ser Gln Asn Arg Ile Val Leu Ala Glu Gly Tyr Glu 
        35                  40                  45              
Val Asn Tyr Gln Val Ser Asp Asp Pro Asp Ala Val Tyr Gly Tyr Leu 
    50                  55                  60                  
Ser Ile Pro Ser Leu Glu Ile Met Glu Pro Val Tyr Leu Gly Ala Asp 
65                  70                  75                  80  
Tyr His His Leu Gly Met Gly Leu Ala His Val Asp Gly Thr Pro Leu 
                85                  90                  95      
Pro Leu Asp Gly Thr Gly Ile Arg Ser Val Ile Ala Gly His Arg Ala 
            100                 105                 110         
Glu Pro Ser His Val Phe Phe Arg His Leu Asp Gln Leu Lys Val Gly 
        115                 120                 125             
Asp Ala Leu Tyr Tyr Asp Asn Gly Gln Glu Ile Val Glu Tyr Gln Met 
    130                 135                 140                 
Met Asp Thr Glu Ile Ile Leu Pro Ser Glu Trp Glu Lys Leu Glu Ser 
145                 150                 155                 160 
Val Ser Ser Lys Asn Ile Met Thr Leu Ile Thr Cys Asp Pro Ile Pro 
                165                 170                 175     
Thr Phe Asn Lys Arg Leu Leu Val Asn Phe Glu Arg Val Ala Val Tyr 
            180                 185                 190         
Gln Lys Ser Asp Pro Gln Thr Ala Ala Val Ala Arg Val Ala Phe Thr 
        195                 200                 205             
Lys Glu Gly Gln Ser Val Ser Arg Val Ala Thr Ser Gln Trp Leu Tyr 
    210                 215                 220                 
Arg Gly Leu Val Val Leu Ala Phe Leu Gly Ile Leu Phe Val Leu Trp 
225                 230                 235                 240 
Lys Leu Ala Arg Leu Leu Arg Gly Lys 
                245                 

<210> 36
<211> 244
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GAS sortase C1 deletion mutant

<400> 36
Arg Asp Arg Gln Leu Leu Ser Thr Tyr His Lys Gln Val Thr Gln Lys 
1               5                   10                  15      
Lys Pro Ser Glu Met Glu Glu Val Trp Gln Lys Ala Lys Ala Tyr Asn 
            20                  25                  30          
Ala Arg Leu Gly Ile Gln Pro Val Pro Ser Phe Arg Asp Gly Ile His 
        35                  40                  45              
Asp Lys Asn Tyr Glu Ser Leu Leu Gln Ile Glu Asn Asn Asp Ile Met 
    50                  55                  60                  
Gly Tyr Val Glu Val Pro Ser Ile Lys Val Thr Leu Pro Ile Tyr His 
65                  70                  75                  80  
Tyr Thr Thr Asp Glu Val Leu Thr Lys Gly Ala Gly His Leu Phe Gly 
                85                  90                  95      
Ser Ala Leu Pro Val Gly Gly Asp Gly Thr His Thr Val Ile Ser Ala 
            100                 105                 110         
His Arg Gly Leu Pro Ser Ala Glu Met Phe Thr Asn Leu Asn Leu Val 
        115                 120                 125             
Lys Lys Gly Asp Thr Phe Tyr Phe Arg Val Leu Asn Lys Val Leu Ala 
    130                 135                 140                 
Tyr Lys Val Asp Gln Ile Leu Thr Val Glu Pro Asp Gln Val Thr Ser 
145                 150                 155                 160 
Leu Ser Gly Val Met Gly Lys Asp Tyr Ala Thr Leu Val Thr Cys Thr 
                165                 170                 175     
Pro Tyr Gly Val Asn Thr Lys Arg Leu Leu Val Arg Gly His Arg Ile 
            180                 185                 190         
Ala Tyr His Tyr Lys Lys Tyr Gln Gln Ala Lys Lys Ala Met Lys Leu 
        195                 200                 205             
Val Asp Lys Ser Arg Met Trp Ala Glu Val Val Cys Ala Ala Phe Gly 
    210                 215                 220                 
Val Val Ile Ala Ile Ile Leu Val Phe Met Tyr Ser Arg Val Ser Ala 
225                 230                 235                 240 
Lys Lys Ser Lys 
                

<210> 37
<211> 273
<212> PRT
<213> Streptococcus pyogenes

<220> 
<223> GBS sortase C1 of PI-1 substitution mutant

<400> 37
Ile Val Ser Gln Val Met Tyr Phe Gln Ala Ser His Ala Asn Ile Asn 
1               5                   10                  15      
Ala Phe Lys Glu Ala Val Thr Lys Ile Asp Arg Val Glu Ile Asn Arg 
            20                  25                  30          
Arg Leu Glu Leu Ala Tyr Ala Tyr Asn Ala Ser Ile Ala Gly Ala Lys 
        35                  40                  45              
Thr Asn Gly Glu Tyr Pro Ala Leu Lys Ala Pro Tyr Ser Ala Glu Gln 
    50                  55                  60                  
Lys Gln Ala Gly Val Val Glu Tyr Ala Arg Met Leu Glu Val Lys Glu 
65                  70                  75                  80  
Gln Ile Gly His Val Ile Ile Pro Arg Ile Asn Gln Asp Ile Pro Ile 
                85                  90                  95      
Tyr Ala Gly Ser Ala Glu Glu Asn Leu Gln Arg Gly Val Gly His Leu 
            100                 105                 110         
Glu Gly Thr Ser Leu Pro Val Gly Gly Glu Ser Thr His Ala Val Leu 
        115                 120                 125             
Thr Ala His Arg Gly Leu Pro Thr Ala Lys Leu Phe Thr Asn Leu Asp 
    130                 135                 140                 
Lys Val Thr Val Gly Asp Arg Phe Tyr Ile Glu His Ile Gly Gly Lys 
145                 150                 155                 160 
Ile Ala Tyr Gln Val Asp Gln Ile Lys Val Ile Ala Pro Asp Gln Leu 
                165                 170                 175     
Glu Asp Leu Tyr Val Ile Gln Gly Glu Asp His Val Thr Leu Leu Thr 
            180                 185                 190         
Cys Thr Pro Tyr Met Ile Asn Ser His Arg Leu Leu Val Arg Gly Lys 
        195                 200                 205             
Arg Ile Pro Tyr Val Glu Lys Thr Val Gln Lys Asp Ser Lys Thr Phe 
    210                 215                 220                 
Arg Gln Gln Gln Tyr Leu Thr Tyr Ala Met Trp Val Val Val Gly Leu 
225                 230                 235                 240 
Ile Leu Leu Ser Leu Leu Ile Trp Phe Lys Lys Thr Lys Gln Lys Lys 
                245                 250                 255     
Arg Arg Lys Asn Glu Lys Ala Ala Ser Gln Asn Ser His Asn Asn Ser 
            260                 265                 270         
Lys 
    

<210> 38
<211> 242
<212> PRT
<213> Streptococcus pyogenes

<220> 
<223> GBS sortase C2 of PI-1 substitution mutant

<400> 38
Ala Ser His Gln Asn Ile Asn Gln Phe Lys Arg Glu Val Ala Lys Ile 
1               5                   10                  15      
Asp Thr Asn Thr Val Glu Arg Arg Ile Ala Leu Ala Asn Ala Tyr Asn 
            20                  25                  30          
Glu Thr Leu Ser Arg Asn Pro Leu Leu Ile Ala Pro Phe Thr Ser Lys 
        35                  40                  45              
Gln Lys Glu Gly Leu Arg Glu Tyr Ala Arg Met Leu Glu Val His Glu 
    50                  55                  60                  
Gln Ile Gly His Val Ala Ile Pro Ser Ile Gly Val Asp Ile Pro Ile 
65                  70                  75                  80  
Tyr Ala Gly Thr Ser Glu Thr Val Leu Gln Lys Gly Ser Gly His Leu 
                85                  90                  95      
Glu Gly Thr Ser Leu Pro Val Gly Gly Leu Ser Thr His Ser Val Leu 
            100                 105                 110         
Thr Ala His Arg Gly Leu Pro Thr Ala Arg Leu Phe Thr Asp Leu Asn 
        115                 120                 125             
Lys Val Lys Lys Gly Gln Ile Phe Tyr Val Thr Asn Ile Lys Glu Thr 
    130                 135                 140                 
Leu Ala Tyr Lys Val Val Ser Ile Lys Val Val Asp Pro Thr Ala Leu 
145                 150                 155                 160 
Ser Glu Val Lys Ile Val Asn Gly Lys Asp Tyr Ile Thr Leu Leu Thr 
                165                 170                 175     
Cys Thr Pro Tyr Met Ile Asn Ser His Arg Leu Leu Val Lys Gly Glu 
            180                 185                 190         
Arg Ile Pro Tyr Asp Ser Thr Glu Ala Glu Lys His Lys Glu Gln Thr 
        195                 200                 205             
Val Gln Asp Tyr Arg Leu Ser Leu Val Leu Lys Ile Leu Leu Val Leu 
    210                 215                 220                 
Leu Ile Gly Leu Phe Ile Val Ile Met Met Arg Arg Trp Met Gln His 
225                 230                 235                 240 
Arg Gln 
        

<210> 39
<211> 250
<212> PRT
<213> Streptococcus pyogenes

<220> 
<223> GBS sortase C1 of PI-2a substitution mutant

<400> 39
Glu Ser Asn Asn Gln Thr Gln Asp Phe Glu Arg Ala Ala Lys Lys Leu 
1               5                   10                  15      
Ser Gln Lys Glu Ile Asn Arg Arg Met Ala Leu Ala Gln Ala Tyr Asn 
            20                  25                  30          
Asp Ser Leu Asn Asn Val His Leu Glu Ala Pro Tyr Glu Lys Lys Arg 
        35                  40                  45              
Ile Gln Lys Gly Ile Ala Glu Tyr Ala Arg Met Leu Glu Val Ser Glu 
    50                  55                  60                  
Lys Ile Gly Ile Ile Ser Val Pro Lys Ile Gly Gln Lys Leu Pro Ile 
65                  70                  75                  80  
Phe Ala Gly Ser Ser Gln Glu Val Leu Ser Lys Gly Ala Gly His Leu 
                85                  90                  95      
Glu Gly Thr Ser Leu Pro Ile Gly Gly Asn Ser Thr His Thr Val Ile 
            100                 105                 110         
Thr Ala His Ser Gly Ile Pro Asp Lys Glu Leu Phe Ser Asn Leu Lys 
        115                 120                 125             
Lys Leu Lys Lys Gly Asp Lys Phe Tyr Ile Gln Asn Ile Lys Glu Thr 
    130                 135                 140                 
Ile Ala Tyr Gln Val Asp Gln Ile Lys Val Val Thr Pro Asp Asn Phe 
145                 150                 155                 160 
Ser Asp Leu Leu Val Val Pro Gly His Asp Tyr Ala Thr Leu Leu Thr 
                165                 170                 175     
Cys Thr Pro Ile Met Val Asn Thr His Arg Leu Leu Val Arg Gly His 
            180                 185                 190         
Arg Ile Pro Tyr Lys Gly Pro Ile Asp Glu Lys Leu Ile Lys Asp Gly 
        195                 200                 205             
His Leu Asn Thr Ile Tyr Arg Tyr Leu Phe Tyr Ile Ser Leu Val Ile 
    210                 215                 220                 
Ile Ala Trp Leu Leu Trp Leu Ile Lys Arg Gln Arg Gln Lys Asn Arg 
225                 230                 235                 240 
Leu Ser Ser Val Arg Lys Gly Ile Glu Ser 
                245                 250 

<210> 40
<211> 248
<212> PRT
<213> Streptococcus pyogenes

<220> 
<223> GBS sortase C2 of PI-2a substitution mutant

<400> 40
Lys Ala Asn Asn Gln Val Thr Asn Phe Asp Asn Gln Thr Gln Lys Leu 
1               5                   10                  15      
Asn Ala Lys Glu Ile Asn Arg Arg Phe Glu Leu Ala Lys Ala Tyr Asn 
            20                  25                  30          
Arg Thr Leu Asp Pro Ser Arg Leu Ser Ala Pro Tyr Thr Glu Lys Glu 
        35                  40                  45              
Lys Lys Gly Ile Ala Glu Tyr Ala His Met Leu Glu Ile Thr Glu Met 
    50                  55                  60                  
Ile Gly Tyr Ile Asp Ile Pro Ser Ile Lys Gln Lys Leu Pro Ile Tyr 
65                  70                  75                  80  
Ala Gly Thr Thr Ser Ser Val Leu Glu Lys Gly Ser Gly His Leu Glu 
                85                  90                  95      
Gly Thr Ser Leu Pro Ile Gly Gly Lys Ser Ser His Thr Val Ile Thr 
            100                 105                 110         
Ala His Arg Gly Leu Pro Lys Ala Lys Leu Phe Thr Asp Leu Asp Lys 
        115                 120                 125             
Leu Lys Lys Gly Lys Ile Phe Tyr Ile His Asn Ile Lys Glu Val Leu 
    130                 135                 140                 
Ala Tyr Lys Val Asp Gln Ile Ser Val Val Lys Pro Asp Asn Phe Ser 
145                 150                 155                 160 
Lys Leu Leu Val Val Lys Gly Lys Asp Tyr Ala Thr Leu Leu Thr Cys 
                165                 170                 175     
Thr Pro Tyr Ser Ile Asn Ser His Arg Leu Leu Val Arg Gly His Arg 
            180                 185                 190         
Ile Lys Tyr Val Pro Pro Val Lys Glu Lys Asn Tyr Leu Met Lys Glu 
        195                 200                 205             
Leu Gln Thr His Tyr Lys Leu Tyr Phe Leu Leu Ser Ile Leu Val Ile 
    210                 215                 220                 
Leu Ile Leu Val Ala Leu Leu Leu Tyr Leu Lys Arg Lys Phe Lys Glu 
225                 230                 235                 240 
Arg Lys Arg Lys Gly Asn Gln Lys 
                245             

<210> 41
<211> 255
<212> PRT
<213> Streptococcus pyogenes

<220> 
<223> GBS sortase C1 of PI-2b substitution mutant

<400> 41
His Gln Ser Arg Ala Ile Met Asp Tyr Gln Asp Arg Val Thr His Met 
1               5                   10                  15      
Asp Glu Asn Asp Tyr Lys Lys Ile Ile Asn Arg Ala Lys Glu Tyr Asn 
            20                  25                  30          
Lys Gln Phe Lys Thr Ser Gly Ala Lys Trp His Met Thr Ser Gln Glu 
        35                  40                  45              
Arg Leu Asp Tyr Asn Ser Gln Leu Ala Ile Asp Lys Thr Gly Asn Met 
    50                  55                  60                  
Gly Tyr Ile Ser Ile Pro Lys Ile Asn Ile Lys Leu Pro Leu Tyr His 
65                  70                  75                  80  
Gly Thr Ser Glu Lys Val Leu Gln Thr Ser Ile Gly His Leu Glu Gly 
                85                  90                  95      
Ser Ser Leu Pro Ile Gly Gly Asp Ser Thr His Ser Ile Leu Ser Gly 
            100                 105                 110         
His Arg Gly Leu Pro Ser Ser Arg Leu Phe Ser Asp Leu Asp Lys Leu 
        115                 120                 125             
Lys Val Gly Asp His Trp Thr Val Ser Ile Leu Asn Glu Thr Tyr Thr 
    130                 135                 140                 
Tyr Gln Val Asp Gln Ile Arg Thr Val Lys Pro Asp Asp Leu Arg Asp 
145                 150                 155                 160 
Leu Gln Ile Val Lys Gly Lys Asp Tyr Gln Thr Leu Val Thr Cys Thr 
                165                 170                 175     
Pro Tyr Gly Val Asn Thr His Arg Leu Leu Val Arg Gly His Arg Val 
            180                 185                 190         
Pro Asn Asp Asn Gly Asn Ala Leu Val Val Ala Glu Ala Ile Gln Ile 
        195                 200                 205             
Glu Pro Ile Tyr Ile Ala Pro Phe Ile Ala Ile Phe Leu Thr Leu Ile 
    210                 215                 220                 
Leu Leu Leu Ile Ser Leu Glu Val Thr Arg Arg Ala Arg Gln Arg Lys 
225                 230                 235                 240 
Lys Ile Leu Lys Gln Ala Met Arg Lys Glu Glu Asn Asn Asp Leu 
                245                 250                 255 

<210> 42
<211> 263
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C1 substitution mutant

<400> 42
Glu Ser Asn Gln Gln Ile Ala Asp Phe Asp Lys Glu Lys Ala Thr Leu 
1               5                   10                  15      
Asp Glu Ala Asp Ile Asp Glu Arg Met Lys Leu Ala Gln Ala Phe Asn 
            20                  25                  30          
Asp Ser Leu Asn Asn Val Val Ser Gly Ala Pro Trp Ser Glu Glu Met 
        35                  40                  45              
Lys Lys Lys Gly Arg Ala Glu Tyr Ala Arg Met Leu Glu Ile His Glu 
    50                  55                  60                  
Arg Met Gly His Val Glu Ile Pro Val Ile Asp Val Asp Leu Pro Val 
65                  70                  75                  80  
Tyr Ala Gly Thr Ala Glu Glu Val Leu Gln Gln Gly Ala Gly His Leu 
                85                  90                  95      
Glu Gly Thr Ser Leu Pro Ile Gly Gly Asn Ser Thr His Ala Val Ile 
            100                 105                 110         
Thr Ala His Thr Gly Leu Pro Thr Ala Lys Met Phe Thr Asp Leu Thr 
        115                 120                 125             
Lys Leu Lys Val Gly Asp Lys Phe Tyr Val His Asn Ile Lys Glu Val 
    130                 135                 140                 
Met Ala Tyr Gln Val Asp Gln Val Lys Val Ile Glu Pro Thr Asn Phe 
145                 150                 155                 160 
Asp Asp Leu Leu Ile Val Pro Gly His Asp Tyr Val Thr Leu Leu Thr 
                165                 170                 175     
Cys Thr Pro Tyr Met Ile Asn Thr His Arg Leu Leu Val Arg Gly His 
            180                 185                 190         
Arg Ile Pro Tyr Val Ala Glu Val Glu Glu Glu Phe Ile Ala Ala Asn 
        195                 200                 205             
Lys Leu Ser His Leu Tyr Arg Tyr Leu Phe Tyr Val Ala Val Gly Leu 
    210                 215                 220                 
Ile Val Ile Leu Leu Trp Ile Ile Arg Arg Leu Arg Lys Lys Lys Lys 
225                 230                 235                 240 
Gln Pro Glu Lys Ala Leu Lys Ala Leu Lys Ala Ala Arg Lys Glu Val 
                245                 250                 255     
Lys Val Glu Asp Gly Gln Gln 
            260             

<210> 43
<211> 253
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C2 substitution mutant

<400> 43
Glu Ser Asn Glu Val Ile Lys Glu Phe Asp Glu Thr Val Ser Gln Met 
1               5                   10                  15      
Asp Lys Ala Glu Leu Glu Glu Arg Trp Arg Leu Ala Gln Ala Phe Asn 
            20                  25                  30          
Ala Thr Leu Lys Pro Ser Glu Ile Leu Ala Pro Phe Thr Glu Gln Glu 
        35                  40                  45              
Lys Lys Lys Gly Val Ser Glu Tyr Ala Asn Met Leu Lys Val His Glu 
    50                  55                  60                  
Arg Ile Gly Tyr Val Glu Ile Pro Ala Ile Asp Gln Glu Ile Pro Met 
65                  70                  75                  80  
Tyr Val Gly Thr Ser Glu Asp Ile Leu Gln Lys Gly Ala Gly Leu Leu 
                85                  90                  95      
Glu Gly Ala Ser Leu Pro Val Gly Gly Glu Asn Thr His Thr Val Ile 
            100                 105                 110         
Thr Ala His Arg Gly Leu Pro Thr Ala Glu Leu Phe Ser Gln Leu Asp 
        115                 120                 125             
Lys Met Lys Lys Gly Asp Ile Phe Tyr Leu His Val Leu Asp Gln Val 
    130                 135                 140                 
Leu Ala Tyr Gln Val Asp Gln Ile Val Thr Val Glu Pro Asn Asp Phe 
145                 150                 155                 160 
Glu Pro Val Leu Ile Gln His Gly Glu Asp Tyr Ala Thr Leu Leu Thr 
                165                 170                 175     
Cys Thr Pro Tyr Met Ile Asn Ser His Arg Leu Leu Val Arg Gly Lys 
            180                 185                 190         
Arg Ile Pro Tyr Thr Ala Pro Ile Ala Glu Arg Asn Arg Ala Val Arg 
        195                 200                 205             
Glu Arg Gly Gln Phe Trp Leu Trp Leu Leu Leu Gly Ala Met Ala Val 
    210                 215                 220                 
Ile Leu Leu Leu Leu Tyr Arg Val Tyr Arg Asn Arg Arg Ile Val Lys 
225                 230                 235                 240 
Gly Leu Glu Lys Gln Leu Glu Gly Arg His Val Lys Asp 
                245                 250             

<210> 44
<211> 252
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C3 substitution mutant

<400> 44
Gln Ser Leu Gly Gln Val Lys Gly His Ala Thr Phe Val Lys Ser Met 
1               5                   10                  15      
Thr Thr Glu Met Tyr Gln Glu Gln Gln Asn His Ser Leu Ala Tyr Asn 
            20                  25                  30          
Gln Arg Leu Ala Ser Gln Asn Arg Ile Val Ala Pro Phe Leu Ala Glu 
        35                  40                  45              
Gly Tyr Glu Val Asn Tyr Gln Val Ser Asp Asp Pro Asp Ala Val Tyr 
    50                  55                  60                  
Gly Tyr Leu Ser Ile Pro Ser Leu Glu Ile Met Glu Pro Val Tyr Leu 
65                  70                  75                  80  
Gly Ala Asp Tyr His His Leu Gly Met Gly Leu Ala His Val Asp Gly 
                85                  90                  95      
Thr Pro Leu Pro Leu Asp Gly Thr Gly Ile Arg Ser Val Ile Ala Gly 
            100                 105                 110         
His Arg Ala Glu Pro Ser His Val Phe Phe Arg His Leu Asp Gln Leu 
        115                 120                 125             
Lys Val Gly Asp Ala Leu Tyr Tyr Asp Asn Gly Gln Glu Ile Val Glu 
    130                 135                 140                 
Tyr Gln Met Met Asp Thr Glu Ile Ile Leu Pro Ser Glu Trp Glu Lys 
145                 150                 155                 160 
Leu Glu Ser Val Ser Ser Lys Asn Ile Met Thr Leu Ile Thr Cys Asp 
                165                 170                 175     
Pro Ile Pro Thr Phe Asn Lys Arg Leu Leu Val Asn Phe Glu Arg Val 
            180                 185                 190         
Ala Val Tyr Gln Lys Ser Asp Pro Gln Thr Ala Ala Val Ala Arg Val 
        195                 200                 205             
Ala Phe Thr Lys Glu Gly Gln Ser Val Ser Arg Val Ala Thr Ser Gln 
    210                 215                 220                 
Trp Leu Tyr Arg Gly Leu Val Val Leu Ala Phe Leu Gly Ile Leu Phe 
225                 230                 235                 240 
Val Leu Trp Lys Leu Ala Arg Leu Leu Arg Gly Lys 
                245                 250         

<210> 45
<211> 247
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GAS sortase C1 substitution mutant

<400> 45
Arg Asp Arg Gln Leu Leu Ser Thr Tyr His Lys Gln Val Thr Gln Lys 
1               5                   10                  15      
Lys Pro Ser Glu Met Glu Glu Val Trp Gln Lys Ala Lys Ala Tyr Asn 
            20                  25                  30          
Ala Arg Leu Gly Ile Gln Pro Val Pro Ala Ala Phe Ser Phe Arg Asp 
        35                  40                  45              
Gly Ile His Asp Lys Asn Tyr Glu Ser Leu Leu Gln Ile Glu Asn Asn 
    50                  55                  60                  
Asp Ile Met Gly Tyr Val Glu Val Pro Ser Ile Lys Val Thr Leu Pro 
65                  70                  75                  80  
Ile Tyr His Tyr Thr Thr Asp Glu Val Leu Thr Lys Gly Ala Gly His 
                85                  90                  95      
Leu Phe Gly Ser Ala Leu Pro Val Gly Gly Asp Gly Thr His Thr Val 
            100                 105                 110         
Ile Ser Ala His Arg Gly Leu Pro Ser Ala Glu Met Phe Thr Asn Leu 
        115                 120                 125             
Asn Leu Val Lys Lys Gly Asp Thr Phe Tyr Phe Arg Val Leu Asn Lys 
    130                 135                 140                 
Val Leu Ala Tyr Lys Val Asp Gln Ile Leu Thr Val Glu Pro Asp Gln 
145                 150                 155                 160 
Val Thr Ser Leu Ser Gly Val Met Gly Lys Asp Tyr Ala Thr Leu Val 
                165                 170                 175     
Thr Cys Thr Pro Tyr Gly Val Asn Thr Lys Arg Leu Leu Val Arg Gly 
            180                 185                 190         
His Arg Ile Ala Tyr His Tyr Lys Lys Tyr Gln Gln Ala Lys Lys Ala 
        195                 200                 205             
Met Lys Leu Val Asp Lys Ser Arg Met Trp Ala Glu Val Val Cys Ala 
    210                 215                 220                 
Ala Phe Gly Val Val Ile Ala Ile Ile Leu Val Phe Met Tyr Ser Arg 
225                 230                 235                 240 
Val Ser Ala Lys Lys Ser Lys 
                245         

<210> 46
<211> 273
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS sortase C1 of PI-1 substitution mutant

<400> 46
Ile Val Ser Gln Val Met Tyr Phe Gln Ala Ser His Ala Asn Ile Asn 
1               5                   10                  15      
Ala Phe Lys Glu Ala Val Thr Lys Ile Asp Arg Val Glu Ile Asn Arg 
            20                  25                  30          
Arg Leu Glu Leu Ala Tyr Ala Tyr Asn Ala Ser Ile Ala Gly Ala Lys 
        35                  40                  45              
Thr Asn Gly Glu Tyr Pro Ala Leu Lys Asp Pro Ala Ser Ala Glu Gln 
    50                  55                  60                  
Lys Gln Ala Gly Val Val Glu Tyr Ala Arg Met Leu Glu Val Lys Glu 
65                  70                  75                  80  
Gln Ile Gly His Val Ile Ile Pro Arg Ile Asn Gln Asp Ile Pro Ile 
                85                  90                  95      
Tyr Ala Gly Ser Ala Glu Glu Asn Leu Gln Arg Gly Val Gly His Leu 
            100                 105                 110         
Glu Gly Thr Ser Leu Pro Val Gly Gly Glu Ser Thr His Ala Val Leu 
        115                 120                 125             
Thr Ala His Arg Gly Leu Pro Thr Ala Lys Leu Phe Thr Asn Leu Asp 
    130                 135                 140                 
Lys Val Thr Val Gly Asp Arg Phe Tyr Ile Glu His Ile Gly Gly Lys 
145                 150                 155                 160 
Ile Ala Tyr Gln Val Asp Gln Ile Lys Val Ile Ala Pro Asp Gln Leu 
                165                 170                 175     
Glu Asp Leu Tyr Val Ile Gln Gly Glu Asp His Val Thr Leu Leu Thr 
            180                 185                 190         
Cys Thr Pro Tyr Met Ile Asn Ser His Arg Leu Leu Val Arg Gly Lys 
        195                 200                 205             
Arg Ile Pro Tyr Val Glu Lys Thr Val Gln Lys Asp Ser Lys Thr Phe 
    210                 215                 220                 
Arg Gln Gln Gln Tyr Leu Thr Tyr Ala Met Trp Val Val Val Gly Leu 
225                 230                 235                 240 
Ile Leu Leu Ser Leu Leu Ile Trp Phe Lys Lys Thr Lys Gln Lys Lys 
                245                 250                 255     
Arg Arg Lys Asn Glu Lys Ala Ala Ser Gln Asn Ser His Asn Asn Ser 
            260                 265                 270         
Lys 
    

<210> 47
<211> 242
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS sortase C2 of PI-1 substitution mutant

<400> 47
Ala Ser His Gln Asn Ile Asn Gln Phe Lys Arg Glu Val Ala Lys Ile 
1               5                   10                  15      
Asp Thr Asn Thr Val Glu Arg Arg Ile Ala Leu Ala Asn Ala Tyr Asn 
            20                  25                  30          
Glu Thr Leu Ser Arg Asn Pro Leu Leu Ile Asp Pro Ala Thr Ser Lys 
        35                  40                  45              
Gln Lys Glu Gly Leu Arg Glu Tyr Ala Arg Met Leu Glu Val His Glu 
    50                  55                  60                  
Gln Ile Gly His Val Ala Ile Pro Ser Ile Gly Val Asp Ile Pro Ile 
65                  70                  75                  80  
Tyr Ala Gly Thr Ser Glu Thr Val Leu Gln Lys Gly Ser Gly His Leu 
                85                  90                  95      
Glu Gly Thr Ser Leu Pro Val Gly Gly Leu Ser Thr His Ser Val Leu 
            100                 105                 110         
Thr Ala His Arg Gly Leu Pro Thr Ala Arg Leu Phe Thr Asp Leu Asn 
        115                 120                 125             
Lys Val Lys Lys Gly Gln Ile Phe Tyr Val Thr Asn Ile Lys Glu Thr 
    130                 135                 140                 
Leu Ala Tyr Lys Val Val Ser Ile Lys Val Val Asp Pro Thr Ala Leu 
145                 150                 155                 160 
Ser Glu Val Lys Ile Val Asn Gly Lys Asp Tyr Ile Thr Leu Leu Thr 
                165                 170                 175     
Cys Thr Pro Tyr Met Ile Asn Ser His Arg Leu Leu Val Lys Gly Glu 
            180                 185                 190         
Arg Ile Pro Tyr Asp Ser Thr Glu Ala Glu Lys His Lys Glu Gln Thr 
        195                 200                 205             
Val Gln Asp Tyr Arg Leu Ser Leu Val Leu Lys Ile Leu Leu Val Leu 
    210                 215                 220                 
Leu Ile Gly Leu Phe Ile Val Ile Met Met Arg Arg Trp Met Gln His 
225                 230                 235                 240 
Arg Gln 
        

<210> 48
<211> 250
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS sortase C1 of PI-2a substitution mutant

<400> 48
Glu Ser Asn Asn Gln Thr Gln Asp Phe Glu Arg Ala Ala Lys Lys Leu 
1               5                   10                  15      
Ser Gln Lys Glu Ile Asn Arg Arg Met Ala Leu Ala Gln Ala Tyr Asn 
            20                  25                  30          
Asp Ser Leu Asn Asn Val His Leu Glu Asp Pro Ala Glu Lys Lys Arg 
        35                  40                  45              
Ile Gln Lys Gly Ile Ala Glu Tyr Ala Arg Met Leu Glu Val Ser Glu 
    50                  55                  60                  
Lys Ile Gly Ile Ile Ser Val Pro Lys Ile Gly Gln Lys Leu Pro Ile 
65                  70                  75                  80  
Phe Ala Gly Ser Ser Gln Glu Val Leu Ser Lys Gly Ala Gly His Leu 
                85                  90                  95      
Glu Gly Thr Ser Leu Pro Ile Gly Gly Asn Ser Thr His Thr Val Ile 
            100                 105                 110         
Thr Ala His Ser Gly Ile Pro Asp Lys Glu Leu Phe Ser Asn Leu Lys 
        115                 120                 125             
Lys Leu Lys Lys Gly Asp Lys Phe Tyr Ile Gln Asn Ile Lys Glu Thr 
    130                 135                 140                 
Ile Ala Tyr Gln Val Asp Gln Ile Lys Val Val Thr Pro Asp Asn Phe 
145                 150                 155                 160 
Ser Asp Leu Leu Val Val Pro Gly His Asp Tyr Ala Thr Leu Leu Thr 
                165                 170                 175     
Cys Thr Pro Ile Met Val Asn Thr His Arg Leu Leu Val Arg Gly His 
            180                 185                 190         
Arg Ile Pro Tyr Lys Gly Pro Ile Asp Glu Lys Leu Ile Lys Asp Gly 
        195                 200                 205             
His Leu Asn Thr Ile Tyr Arg Tyr Leu Phe Tyr Ile Ser Leu Val Ile 
    210                 215                 220                 
Ile Ala Trp Leu Leu Trp Leu Ile Lys Arg Gln Arg Gln Lys Asn Arg 
225                 230                 235                 240 
Leu Ser Ser Val Arg Lys Gly Ile Glu Ser 
                245                 250 

<210> 49
<211> 248
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS sortase C2 of PI-2a substitution mutant

<400> 49
Lys Ala Asn Asn Gln Val Thr Asn Phe Asp Asn Gln Thr Gln Lys Leu 
1               5                   10                  15      
Asn Ala Lys Glu Ile Asn Arg Arg Phe Glu Leu Ala Lys Ala Tyr Asn 
            20                  25                  30          
Arg Thr Leu Asp Pro Ser Arg Leu Ser Asp Pro Ala Thr Glu Lys Glu 
        35                  40                  45              
Lys Lys Gly Ile Ala Glu Tyr Ala His Met Leu Glu Ile Thr Glu Met 
    50                  55                  60                  
Ile Gly Tyr Ile Asp Ile Pro Ser Ile Lys Gln Lys Leu Pro Ile Tyr 
65                  70                  75                  80  
Ala Gly Thr Thr Ser Ser Val Leu Glu Lys Gly Ser Gly His Leu Glu 
                85                  90                  95      
Gly Thr Ser Leu Pro Ile Gly Gly Lys Ser Ser His Thr Val Ile Thr 
            100                 105                 110         
Ala His Arg Gly Leu Pro Lys Ala Lys Leu Phe Thr Asp Leu Asp Lys 
        115                 120                 125             
Leu Lys Lys Gly Lys Ile Phe Tyr Ile His Asn Ile Lys Glu Val Leu 
    130                 135                 140                 
Ala Tyr Lys Val Asp Gln Ile Ser Val Val Lys Pro Asp Asn Phe Ser 
145                 150                 155                 160 
Lys Leu Leu Val Val Lys Gly Lys Asp Tyr Ala Thr Leu Leu Thr Cys 
                165                 170                 175     
Thr Pro Tyr Ser Ile Asn Ser His Arg Leu Leu Val Arg Gly His Arg 
            180                 185                 190         
Ile Lys Tyr Val Pro Pro Val Lys Glu Lys Asn Tyr Leu Met Lys Glu 
        195                 200                 205             
Leu Gln Thr His Tyr Lys Leu Tyr Phe Leu Leu Ser Ile Leu Val Ile 
    210                 215                 220                 
Leu Ile Leu Val Ala Leu Leu Leu Tyr Leu Lys Arg Lys Phe Lys Glu 
225                 230                 235                 240 
Arg Lys Arg Lys Gly Asn Gln Lys 
                245             

<210> 50
<211> 255
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS sortase C1 of PI-2b substitution mutant

<400> 50
His Gln Ser Arg Ala Ile Met Asp Tyr Gln Asp Arg Val Thr His Met 
1               5                   10                  15      
Asp Glu Asn Asp Tyr Lys Lys Ile Ile Asn Arg Ala Lys Glu Tyr Asn 
            20                  25                  30          
Lys Gln Phe Lys Thr Ser Gly Met Lys Ala His Met Thr Ser Gln Glu 
        35                  40                  45              
Arg Leu Asp Tyr Asn Ser Gln Leu Ala Ile Asp Lys Thr Gly Asn Met 
    50                  55                  60                  
Gly Tyr Ile Ser Ile Pro Lys Ile Asn Ile Lys Leu Pro Leu Tyr His 
65                  70                  75                  80  
Gly Thr Ser Glu Lys Val Leu Gln Thr Ser Ile Gly His Leu Glu Gly 
                85                  90                  95      
Ser Ser Leu Pro Ile Gly Gly Asp Ser Thr His Ser Ile Leu Ser Gly 
            100                 105                 110         
His Arg Gly Leu Pro Ser Ser Arg Leu Phe Ser Asp Leu Asp Lys Leu 
        115                 120                 125             
Lys Val Gly Asp His Trp Thr Val Ser Ile Leu Asn Glu Thr Tyr Thr 
    130                 135                 140                 
Tyr Gln Val Asp Gln Ile Arg Thr Val Lys Pro Asp Asp Leu Arg Asp 
145                 150                 155                 160 
Leu Gln Ile Val Lys Gly Lys Asp Tyr Gln Thr Leu Val Thr Cys Thr 
                165                 170                 175     
Pro Tyr Gly Val Asn Thr His Arg Leu Leu Val Arg Gly His Arg Val 
            180                 185                 190         
Pro Asn Asp Asn Gly Asn Ala Leu Val Val Ala Glu Ala Ile Gln Ile 
        195                 200                 205             
Glu Pro Ile Tyr Ile Ala Pro Phe Ile Ala Ile Phe Leu Thr Leu Ile 
    210                 215                 220                 
Leu Leu Leu Ile Ser Leu Glu Val Thr Arg Arg Ala Arg Gln Arg Lys 
225                 230                 235                 240 
Lys Ile Leu Lys Gln Ala Met Arg Lys Glu Glu Asn Asn Asp Leu 
                245                 250                 255 

<210> 51
<211> 263
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C1 substitution mutant

<400> 51
Glu Ser Asn Gln Gln Ile Ala Asp Phe Asp Lys Glu Lys Ala Thr Leu 
1               5                   10                  15      
Asp Glu Ala Asp Ile Asp Glu Arg Met Lys Leu Ala Gln Ala Phe Asn 
            20                  25                  30          
Asp Ser Leu Asn Asn Val Val Ser Gly Asp Pro Ala Ser Glu Glu Met 
        35                  40                  45              
Lys Lys Lys Gly Arg Ala Glu Tyr Ala Arg Met Leu Glu Ile His Glu 
    50                  55                  60                  
Arg Met Gly His Val Glu Ile Pro Val Ile Asp Val Asp Leu Pro Val 
65                  70                  75                  80  
Tyr Ala Gly Thr Ala Glu Glu Val Leu Gln Gln Gly Ala Gly His Leu 
                85                  90                  95      
Glu Gly Thr Ser Leu Pro Ile Gly Gly Asn Ser Thr His Ala Val Ile 
            100                 105                 110         
Thr Ala His Thr Gly Leu Pro Thr Ala Lys Met Phe Thr Asp Leu Thr 
        115                 120                 125             
Lys Leu Lys Val Gly Asp Lys Phe Tyr Val His Asn Ile Lys Glu Val 
    130                 135                 140                 
Met Ala Tyr Gln Val Asp Gln Val Lys Val Ile Glu Pro Thr Asn Phe 
145                 150                 155                 160 
Asp Asp Leu Leu Ile Val Pro Gly His Asp Tyr Val Thr Leu Leu Thr 
                165                 170                 175     
Cys Thr Pro Tyr Met Ile Asn Thr His Arg Leu Leu Val Arg Gly His 
            180                 185                 190         
Arg Ile Pro Tyr Val Ala Glu Val Glu Glu Glu Phe Ile Ala Ala Asn 
        195                 200                 205             
Lys Leu Ser His Leu Tyr Arg Tyr Leu Phe Tyr Val Ala Val Gly Leu 
    210                 215                 220                 
Ile Val Ile Leu Leu Trp Ile Ile Arg Arg Leu Arg Lys Lys Lys Lys 
225                 230                 235                 240 
Gln Pro Glu Lys Ala Leu Lys Ala Leu Lys Ala Ala Arg Lys Glu Val 
                245                 250                 255     
Lys Val Glu Asp Gly Gln Gln 
            260             

<210> 52
<211> 253
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C2 substitution mutant

<400> 52
Glu Ser Asn Glu Val Ile Lys Glu Phe Asp Glu Thr Val Ser Gln Met 
1               5                   10                  15      
Asp Lys Ala Glu Leu Glu Glu Arg Trp Arg Leu Ala Gln Ala Phe Asn 
            20                  25                  30          
Ala Thr Leu Lys Pro Ser Glu Ile Leu Asp Pro Ala Thr Glu Gln Glu 
        35                  40                  45              
Lys Lys Lys Gly Val Ser Glu Tyr Ala Asn Met Leu Lys Val His Glu 
    50                  55                  60                  
Arg Ile Gly Tyr Val Glu Ile Pro Ala Ile Asp Gln Glu Ile Pro Met 
65                  70                  75                  80  
Tyr Val Gly Thr Ser Glu Asp Ile Leu Gln Lys Gly Ala Gly Leu Leu 
                85                  90                  95      
Glu Gly Ala Ser Leu Pro Val Gly Gly Glu Asn Thr His Thr Val Ile 
            100                 105                 110         
Thr Ala His Arg Gly Leu Pro Thr Ala Glu Leu Phe Ser Gln Leu Asp 
        115                 120                 125             
Lys Met Lys Lys Gly Asp Ile Phe Tyr Leu His Val Leu Asp Gln Val 
    130                 135                 140                 
Leu Ala Tyr Gln Val Asp Gln Ile Val Thr Val Glu Pro Asn Asp Phe 
145                 150                 155                 160 
Glu Pro Val Leu Ile Gln His Gly Glu Asp Tyr Ala Thr Leu Leu Thr 
                165                 170                 175     
Cys Thr Pro Tyr Met Ile Asn Ser His Arg Leu Leu Val Arg Gly Lys 
            180                 185                 190         
Arg Ile Pro Tyr Thr Ala Pro Ile Ala Glu Arg Asn Arg Ala Val Arg 
        195                 200                 205             
Glu Arg Gly Gln Phe Trp Leu Trp Leu Leu Leu Gly Ala Met Ala Val 
    210                 215                 220                 
Ile Leu Leu Leu Leu Tyr Arg Val Tyr Arg Asn Arg Arg Ile Val Lys 
225                 230                 235                 240 
Gly Leu Glu Lys Gln Leu Glu Gly Arg His Val Lys Asp 
                245                 250             

<210> 53
<211> 252
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C3 substitution mutant

<400> 53
Gln Ser Leu Gly Gln Val Lys Gly His Ala Thr Phe Val Lys Ser Met 
1               5                   10                  15      
Thr Thr Glu Met Tyr Gln Glu Gln Gln Asn His Ser Leu Ala Tyr Asn 
            20                  25                  30          
Gln Arg Leu Ala Ser Gln Asn Arg Ile Val Asp Pro Ala Leu Ala Glu 
        35                  40                  45              
Gly Tyr Glu Val Asn Tyr Gln Val Ser Asp Asp Pro Asp Ala Val Tyr 
    50                  55                  60                  
Gly Tyr Leu Ser Ile Pro Ser Leu Glu Ile Met Glu Pro Val Tyr Leu 
65                  70                  75                  80  
Gly Ala Asp Tyr His His Leu Gly Met Gly Leu Ala His Val Asp Gly 
                85                  90                  95      
Thr Pro Leu Pro Leu Asp Gly Thr Gly Ile Arg Ser Val Ile Ala Gly 
            100                 105                 110         
His Arg Ala Glu Pro Ser His Val Phe Phe Arg His Leu Asp Gln Leu 
        115                 120                 125             
Lys Val Gly Asp Ala Leu Tyr Tyr Asp Asn Gly Gln Glu Ile Val Glu 
    130                 135                 140                 
Tyr Gln Met Met Asp Thr Glu Ile Ile Leu Pro Ser Glu Trp Glu Lys 
145                 150                 155                 160 
Leu Glu Ser Val Ser Ser Lys Asn Ile Met Thr Leu Ile Thr Cys Asp 
                165                 170                 175     
Pro Ile Pro Thr Phe Asn Lys Arg Leu Leu Val Asn Phe Glu Arg Val 
            180                 185                 190         
Ala Val Tyr Gln Lys Ser Asp Pro Gln Thr Ala Ala Val Ala Arg Val 
        195                 200                 205             
Ala Phe Thr Lys Glu Gly Gln Ser Val Ser Arg Val Ala Thr Ser Gln 
    210                 215                 220                 
Trp Leu Tyr Arg Gly Leu Val Val Leu Ala Phe Leu Gly Ile Leu Phe 
225                 230                 235                 240 
Val Leu Trp Lys Leu Ala Arg Leu Leu Arg Gly Lys 
                245                 250         

<210> 54
<211> 247
<212> PRT
<213> Streptococcus pyogenes

<220> 
<223> GAS sortase C1 substitution mutant

<400> 54
Arg Asp Arg Gln Leu Leu Ser Thr Tyr His Lys Gln Val Thr Gln Lys 
1               5                   10                  15      
Lys Pro Ser Glu Met Glu Glu Val Trp Gln Lys Ala Lys Ala Tyr Asn 
            20                  25                  30          
Ala Arg Leu Gly Ile Gln Pro Val Pro Asp Ala Ala Ser Phe Arg Asp 
        35                  40                  45              
Gly Ile His Asp Lys Asn Tyr Glu Ser Leu Leu Gln Ile Glu Asn Asn 
    50                  55                  60                  
Asp Ile Met Gly Tyr Val Glu Val Pro Ser Ile Lys Val Thr Leu Pro 
65                  70                  75                  80  
Ile Tyr His Tyr Thr Thr Asp Glu Val Leu Thr Lys Gly Ala Gly His 
                85                  90                  95      
Leu Phe Gly Ser Ala Leu Pro Val Gly Gly Asp Gly Thr His Thr Val 
            100                 105                 110         
Ile Ser Ala His Arg Gly Leu Pro Ser Ala Glu Met Phe Thr Asn Leu 
        115                 120                 125             
Asn Leu Val Lys Lys Gly Asp Thr Phe Tyr Phe Arg Val Leu Asn Lys 
    130                 135                 140                 
Val Leu Ala Tyr Lys Val Asp Gln Ile Leu Thr Val Glu Pro Asp Gln 
145                 150                 155                 160 
Val Thr Ser Leu Ser Gly Val Met Gly Lys Asp Tyr Ala Thr Leu Val 
                165                 170                 175     
Thr Cys Thr Pro Tyr Gly Val Asn Thr Lys Arg Leu Leu Val Arg Gly 
            180                 185                 190         
His Arg Ile Ala Tyr His Tyr Lys Lys Tyr Gln Gln Ala Lys Lys Ala 
        195                 200                 205             
Met Lys Leu Val Asp Lys Ser Arg Met Trp Ala Glu Val Val Cys Ala 
    210                 215                 220                 
Ala Phe Gly Val Val Ile Ala Ile Ile Leu Val Phe Met Tyr Ser Arg 
225                 230                 235                 240 
Val Ser Ala Lys Lys Ser Lys 
                245         

<210> 55
<211> 273
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS sortase C1 of PI-1 substitution mutant

<400> 55
Ile Val Ser Gln Val Met Tyr Phe Gln Ala Ser His Ala Asn Ile Asn 
1               5                   10                  15      
Ala Phe Lys Glu Ala Val Thr Lys Ile Asp Arg Val Glu Ile Asn Arg 
            20                  25                  30          
Arg Leu Glu Leu Ala Tyr Ala Tyr Asn Ala Ser Ile Ala Gly Ala Lys 
        35                  40                  45              
Thr Asn Gly Glu Tyr Pro Ala Leu Lys Ala Pro Ala Ser Ala Glu Gln 
    50                  55                  60                  
Lys Gln Ala Gly Val Val Glu Tyr Ala Arg Met Leu Glu Val Lys Glu 
65                  70                  75                  80  
Gln Ile Gly His Val Ile Ile Pro Arg Ile Asn Gln Asp Ile Pro Ile 
                85                  90                  95      
Tyr Ala Gly Ser Ala Glu Glu Asn Leu Gln Arg Gly Val Gly His Leu 
            100                 105                 110         
Glu Gly Thr Ser Leu Pro Val Gly Gly Glu Ser Thr His Ala Val Leu 
        115                 120                 125             
Thr Ala His Arg Gly Leu Pro Thr Ala Lys Leu Phe Thr Asn Leu Asp 
    130                 135                 140                 
Lys Val Thr Val Gly Asp Arg Phe Tyr Ile Glu His Ile Gly Gly Lys 
145                 150                 155                 160 
Ile Ala Tyr Gln Val Asp Gln Ile Lys Val Ile Ala Pro Asp Gln Leu 
                165                 170                 175     
Glu Asp Leu Tyr Val Ile Gln Gly Glu Asp His Val Thr Leu Leu Thr 
            180                 185                 190         
Cys Thr Pro Tyr Met Ile Asn Ser His Arg Leu Leu Val Arg Gly Lys 
        195                 200                 205             
Arg Ile Pro Tyr Val Glu Lys Thr Val Gln Lys Asp Ser Lys Thr Phe 
    210                 215                 220                 
Arg Gln Gln Gln Tyr Leu Thr Tyr Ala Met Trp Val Val Val Gly Leu 
225                 230                 235                 240 
Ile Leu Leu Ser Leu Leu Ile Trp Phe Lys Lys Thr Lys Gln Lys Lys 
                245                 250                 255     
Arg Arg Lys Asn Glu Lys Ala Ala Ser Gln Asn Ser His Asn Asn Ser 
            260                 265                 270         
Lys 
    

<210> 56
<211> 242
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS sortase C2 of PI-1 substitution mutant

<400> 56
Ala Ser His Gln Asn Ile Asn Gln Phe Lys Arg Glu Val Ala Lys Ile 
1               5                   10                  15      
Asp Thr Asn Thr Val Glu Arg Arg Ile Ala Leu Ala Asn Ala Tyr Asn 
            20                  25                  30          
Glu Thr Leu Ser Arg Asn Pro Leu Leu Ile Ala Pro Ala Thr Ser Lys 
        35                  40                  45              
Gln Lys Glu Gly Leu Arg Glu Tyr Ala Arg Met Leu Glu Val His Glu 
    50                  55                  60                  
Gln Ile Gly His Val Ala Ile Pro Ser Ile Gly Val Asp Ile Pro Ile 
65                  70                  75                  80  
Tyr Ala Gly Thr Ser Glu Thr Val Leu Gln Lys Gly Ser Gly His Leu 
                85                  90                  95      
Glu Gly Thr Ser Leu Pro Val Gly Gly Leu Ser Thr His Ser Val Leu 
            100                 105                 110         
Thr Ala His Arg Gly Leu Pro Thr Ala Arg Leu Phe Thr Asp Leu Asn 
        115                 120                 125             
Lys Val Lys Lys Gly Gln Ile Phe Tyr Val Thr Asn Ile Lys Glu Thr 
    130                 135                 140                 
Leu Ala Tyr Lys Val Val Ser Ile Lys Val Val Asp Pro Thr Ala Leu 
145                 150                 155                 160 
Ser Glu Val Lys Ile Val Asn Gly Lys Asp Tyr Ile Thr Leu Leu Thr 
                165                 170                 175     
Cys Thr Pro Tyr Met Ile Asn Ser His Arg Leu Leu Val Lys Gly Glu 
            180                 185                 190         
Arg Ile Pro Tyr Asp Ser Thr Glu Ala Glu Lys His Lys Glu Gln Thr 
        195                 200                 205             
Val Gln Asp Tyr Arg Leu Ser Leu Val Leu Lys Ile Leu Leu Val Leu 
    210                 215                 220                 
Leu Ile Gly Leu Phe Ile Val Ile Met Met Arg Arg Trp Met Gln His 
225                 230                 235                 240 
Arg Gln 
        

<210> 57
<211> 250
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS sortase C1 of PI-2a substitution mutant

<400> 57
Glu Ser Asn Asn Gln Thr Gln Asp Phe Glu Arg Ala Ala Lys Lys Leu 
1               5                   10                  15      
Ser Gln Lys Glu Ile Asn Arg Arg Met Ala Leu Ala Gln Ala Tyr Asn 
            20                  25                  30          
Asp Ser Leu Asn Asn Val His Leu Glu Ala Pro Ala Glu Lys Lys Arg 
        35                  40                  45              
Ile Gln Lys Gly Ile Ala Glu Tyr Ala Arg Met Leu Glu Val Ser Glu 
    50                  55                  60                  
Lys Ile Gly Ile Ile Ser Val Pro Lys Ile Gly Gln Lys Leu Pro Ile 
65                  70                  75                  80  
Phe Ala Gly Ser Ser Gln Glu Val Leu Ser Lys Gly Ala Gly His Leu 
                85                  90                  95      
Glu Gly Thr Ser Leu Pro Ile Gly Gly Asn Ser Thr His Thr Val Ile 
            100                 105                 110         
Thr Ala His Ser Gly Ile Pro Asp Lys Glu Leu Phe Ser Asn Leu Lys 
        115                 120                 125             
Lys Leu Lys Lys Gly Asp Lys Phe Tyr Ile Gln Asn Ile Lys Glu Thr 
    130                 135                 140                 
Ile Ala Tyr Gln Val Asp Gln Ile Lys Val Val Thr Pro Asp Asn Phe 
145                 150                 155                 160 
Ser Asp Leu Leu Val Val Pro Gly His Asp Tyr Ala Thr Leu Leu Thr 
                165                 170                 175     
Cys Thr Pro Ile Met Val Asn Thr His Arg Leu Leu Val Arg Gly His 
            180                 185                 190         
Arg Ile Pro Tyr Lys Gly Pro Ile Asp Glu Lys Leu Ile Lys Asp Gly 
        195                 200                 205             
His Leu Asn Thr Ile Tyr Arg Tyr Leu Phe Tyr Ile Ser Leu Val Ile 
    210                 215                 220                 
Ile Ala Trp Leu Leu Trp Leu Ile Lys Arg Gln Arg Gln Lys Asn Arg 
225                 230                 235                 240 
Leu Ser Ser Val Arg Lys Gly Ile Glu Ser 
                245                 250 

<210> 58
<211> 248
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS sortase C2 of PI-2a substitution mutant

<400> 58
Lys Ala Asn Asn Gln Val Thr Asn Phe Asp Asn Gln Thr Gln Lys Leu 
1               5                   10                  15      
Asn Ala Lys Glu Ile Asn Arg Arg Phe Glu Leu Ala Lys Ala Tyr Asn 
            20                  25                  30          
Arg Thr Leu Asp Pro Ser Arg Leu Ser Ala Pro Ala Thr Glu Lys Glu 
        35                  40                  45              
Lys Lys Gly Ile Ala Glu Tyr Ala His Met Leu Glu Ile Thr Glu Met 
    50                  55                  60                  
Ile Gly Tyr Ile Asp Ile Pro Ser Ile Lys Gln Lys Leu Pro Ile Tyr 
65                  70                  75                  80  
Ala Gly Thr Thr Ser Ser Val Leu Glu Lys Gly Ser Gly His Leu Glu 
                85                  90                  95      
Gly Thr Ser Leu Pro Ile Gly Gly Lys Ser Ser His Thr Val Ile Thr 
            100                 105                 110         
Ala His Arg Gly Leu Pro Lys Ala Lys Leu Phe Thr Asp Leu Asp Lys 
        115                 120                 125             
Leu Lys Lys Gly Lys Ile Phe Tyr Ile His Asn Ile Lys Glu Val Leu 
    130                 135                 140                 
Ala Tyr Lys Val Asp Gln Ile Ser Val Val Lys Pro Asp Asn Phe Ser 
145                 150                 155                 160 
Lys Leu Leu Val Val Lys Gly Lys Asp Tyr Ala Thr Leu Leu Thr Cys 
                165                 170                 175     
Thr Pro Tyr Ser Ile Asn Ser His Arg Leu Leu Val Arg Gly His Arg 
            180                 185                 190         
Ile Lys Tyr Val Pro Pro Val Lys Glu Lys Asn Tyr Leu Met Lys Glu 
        195                 200                 205             
Leu Gln Thr His Tyr Lys Leu Tyr Phe Leu Leu Ser Ile Leu Val Ile 
    210                 215                 220                 
Leu Ile Leu Val Ala Leu Leu Leu Tyr Leu Lys Arg Lys Phe Lys Glu 
225                 230                 235                 240 
Arg Lys Arg Lys Gly Asn Gln Lys 
                245             

<210> 59
<211> 255
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS sortase C1 of PI-2b substitution mutant

<400> 59
His Gln Ser Arg Ala Ile Met Asp Tyr Gln Asp Arg Val Thr His Met 
1               5                   10                  15      
Asp Glu Asn Asp Tyr Lys Lys Ile Ile Asn Arg Ala Lys Glu Tyr Asn 
            20                  25                  30          
Lys Gln Phe Lys Thr Ser Gly Ala Lys Ala His Met Thr Ser Gln Glu 
        35                  40                  45              
Arg Leu Asp Tyr Asn Ser Gln Leu Ala Ile Asp Lys Thr Gly Asn Met 
    50                  55                  60                  
Gly Tyr Ile Ser Ile Pro Lys Ile Asn Ile Lys Leu Pro Leu Tyr His 
65                  70                  75                  80  
Gly Thr Ser Glu Lys Val Leu Gln Thr Ser Ile Gly His Leu Glu Gly 
                85                  90                  95      
Ser Ser Leu Pro Ile Gly Gly Asp Ser Thr His Ser Ile Leu Ser Gly 
            100                 105                 110         
His Arg Gly Leu Pro Ser Ser Arg Leu Phe Ser Asp Leu Asp Lys Leu 
        115                 120                 125             
Lys Val Gly Asp His Trp Thr Val Ser Ile Leu Asn Glu Thr Tyr Thr 
    130                 135                 140                 
Tyr Gln Val Asp Gln Ile Arg Thr Val Lys Pro Asp Asp Leu Arg Asp 
145                 150                 155                 160 
Leu Gln Ile Val Lys Gly Lys Asp Tyr Gln Thr Leu Val Thr Cys Thr 
                165                 170                 175     
Pro Tyr Gly Val Asn Thr His Arg Leu Leu Val Arg Gly His Arg Val 
            180                 185                 190         
Pro Asn Asp Asn Gly Asn Ala Leu Val Val Ala Glu Ala Ile Gln Ile 
        195                 200                 205             
Glu Pro Ile Tyr Ile Ala Pro Phe Ile Ala Ile Phe Leu Thr Leu Ile 
    210                 215                 220                 
Leu Leu Leu Ile Ser Leu Glu Val Thr Arg Arg Ala Arg Gln Arg Lys 
225                 230                 235                 240 
Lys Ile Leu Lys Gln Ala Met Arg Lys Glu Glu Asn Asn Asp Leu 
                245                 250                 255 

<210> 60
<211> 263
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C1 substitution mutant

<400> 60
Glu Ser Asn Gln Gln Ile Ala Asp Phe Asp Lys Glu Lys Ala Thr Leu 
1               5                   10                  15      
Asp Glu Ala Asp Ile Asp Glu Arg Met Lys Leu Ala Gln Ala Phe Asn 
            20                  25                  30          
Asp Ser Leu Asn Asn Val Val Ser Gly Ala Pro Ala Ser Glu Glu Met 
        35                  40                  45              
Lys Lys Lys Gly Arg Ala Glu Tyr Ala Arg Met Leu Glu Ile His Glu 
    50                  55                  60                  
Arg Met Gly His Val Glu Ile Pro Val Ile Asp Val Asp Leu Pro Val 
65                  70                  75                  80  
Tyr Ala Gly Thr Ala Glu Glu Val Leu Gln Gln Gly Ala Gly His Leu 
                85                  90                  95      
Glu Gly Thr Ser Leu Pro Ile Gly Gly Asn Ser Thr His Ala Val Ile 
            100                 105                 110         
Thr Ala His Thr Gly Leu Pro Thr Ala Lys Met Phe Thr Asp Leu Thr 
        115                 120                 125             
Lys Leu Lys Val Gly Asp Lys Phe Tyr Val His Asn Ile Lys Glu Val 
    130                 135                 140                 
Met Ala Tyr Gln Val Asp Gln Val Lys Val Ile Glu Pro Thr Asn Phe 
145                 150                 155                 160 
Asp Asp Leu Leu Ile Val Pro Gly His Asp Tyr Val Thr Leu Leu Thr 
                165                 170                 175     
Cys Thr Pro Tyr Met Ile Asn Thr His Arg Leu Leu Val Arg Gly His 
            180                 185                 190         
Arg Ile Pro Tyr Val Ala Glu Val Glu Glu Glu Phe Ile Ala Ala Asn 
        195                 200                 205             
Lys Leu Ser His Leu Tyr Arg Tyr Leu Phe Tyr Val Ala Val Gly Leu 
    210                 215                 220                 
Ile Val Ile Leu Leu Trp Ile Ile Arg Arg Leu Arg Lys Lys Lys Lys 
225                 230                 235                 240 
Gln Pro Glu Lys Ala Leu Lys Ala Leu Lys Ala Ala Arg Lys Glu Val 
                245                 250                 255     
Lys Val Glu Asp Gly Gln Gln 
            260             

<210> 61
<211> 253
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C2 substitution mutant

<400> 61
Glu Ser Asn Glu Val Ile Lys Glu Phe Asp Glu Thr Val Ser Gln Met 
1               5                   10                  15      
Asp Lys Ala Glu Leu Glu Glu Arg Trp Arg Leu Ala Gln Ala Phe Asn 
            20                  25                  30          
Ala Thr Leu Lys Pro Ser Glu Ile Leu Ala Pro Ala Thr Glu Gln Glu 
        35                  40                  45              
Lys Lys Lys Gly Val Ser Glu Tyr Ala Asn Met Leu Lys Val His Glu 
    50                  55                  60                  
Arg Ile Gly Tyr Val Glu Ile Pro Ala Ile Asp Gln Glu Ile Pro Met 
65                  70                  75                  80  
Tyr Val Gly Thr Ser Glu Asp Ile Leu Gln Lys Gly Ala Gly Leu Leu 
                85                  90                  95      
Glu Gly Ala Ser Leu Pro Val Gly Gly Glu Asn Thr His Thr Val Ile 
            100                 105                 110         
Thr Ala His Arg Gly Leu Pro Thr Ala Glu Leu Phe Ser Gln Leu Asp 
        115                 120                 125             
Lys Met Lys Lys Gly Asp Ile Phe Tyr Leu His Val Leu Asp Gln Val 
    130                 135                 140                 
Leu Ala Tyr Gln Val Asp Gln Ile Val Thr Val Glu Pro Asn Asp Phe 
145                 150                 155                 160 
Glu Pro Val Leu Ile Gln His Gly Glu Asp Tyr Ala Thr Leu Leu Thr 
                165                 170                 175     
Cys Thr Pro Tyr Met Ile Asn Ser His Arg Leu Leu Val Arg Gly Lys 
            180                 185                 190         
Arg Ile Pro Tyr Thr Ala Pro Ile Ala Glu Arg Asn Arg Ala Val Arg 
        195                 200                 205             
Glu Arg Gly Gln Phe Trp Leu Trp Leu Leu Leu Gly Ala Met Ala Val 
    210                 215                 220                 
Ile Leu Leu Leu Leu Tyr Arg Val Tyr Arg Asn Arg Arg Ile Val Lys 
225                 230                 235                 240 
Gly Leu Glu Lys Gln Leu Glu Gly Arg His Val Lys Asp 
                245                 250             

<210> 62
<211> 252
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C3 substitution mutant

<400> 62
Gln Ser Leu Gly Gln Val Lys Gly His Ala Thr Phe Val Lys Ser Met 
1               5                   10                  15      
Thr Thr Glu Met Tyr Gln Glu Gln Gln Asn His Ser Leu Ala Tyr Asn 
            20                  25                  30          
Gln Arg Leu Ala Ser Gln Asn Arg Ile Val Ala Pro Ala Leu Ala Glu 
        35                  40                  45              
Gly Tyr Glu Val Asn Tyr Gln Val Ser Asp Asp Pro Asp Ala Val Tyr 
    50                  55                  60                  
Gly Tyr Leu Ser Ile Pro Ser Leu Glu Ile Met Glu Pro Val Tyr Leu 
65                  70                  75                  80  
Gly Ala Asp Tyr His His Leu Gly Met Gly Leu Ala His Val Asp Gly 
                85                  90                  95      
Thr Pro Leu Pro Leu Asp Gly Thr Gly Ile Arg Ser Val Ile Ala Gly 
            100                 105                 110         
His Arg Ala Glu Pro Ser His Val Phe Phe Arg His Leu Asp Gln Leu 
        115                 120                 125             
Lys Val Gly Asp Ala Leu Tyr Tyr Asp Asn Gly Gln Glu Ile Val Glu 
    130                 135                 140                 
Tyr Gln Met Met Asp Thr Glu Ile Ile Leu Pro Ser Glu Trp Glu Lys 
145                 150                 155                 160 
Leu Glu Ser Val Ser Ser Lys Asn Ile Met Thr Leu Ile Thr Cys Asp 
                165                 170                 175     
Pro Ile Pro Thr Phe Asn Lys Arg Leu Leu Val Asn Phe Glu Arg Val 
            180                 185                 190         
Ala Val Tyr Gln Lys Ser Asp Pro Gln Thr Ala Ala Val Ala Arg Val 
        195                 200                 205             
Ala Phe Thr Lys Glu Gly Gln Ser Val Ser Arg Val Ala Thr Ser Gln 
    210                 215                 220                 
Trp Leu Tyr Arg Gly Leu Val Val Leu Ala Phe Leu Gly Ile Leu Phe 
225                 230                 235                 240 
Val Leu Trp Lys Leu Ala Arg Leu Leu Arg Gly Lys 
                245                 250         

<210> 63
<211> 247
<212> PRT
<213> Streptococcus pyogenes

<220> 
<223> GAS sortase C1 substitution mutant

<400> 63
Arg Asp Arg Gln Leu Leu Ser Thr Tyr His Lys Gln Val Thr Gln Lys 
1               5                   10                  15      
Lys Pro Ser Glu Met Glu Glu Val Trp Gln Lys Ala Lys Ala Tyr Asn 
            20                  25                  30          
Ala Arg Leu Gly Ile Gln Pro Val Pro Ala Ala Ala Ser Phe Arg Asp 
        35                  40                  45              
Gly Ile His Asp Lys Asn Tyr Glu Ser Leu Leu Gln Ile Glu Asn Asn 
    50                  55                  60                  
Asp Ile Met Gly Tyr Val Glu Val Pro Ser Ile Lys Val Thr Leu Pro 
65                  70                  75                  80  
Ile Tyr His Tyr Thr Thr Asp Glu Val Leu Thr Lys Gly Ala Gly His 
                85                  90                  95      
Leu Phe Gly Ser Ala Leu Pro Val Gly Gly Asp Gly Thr His Thr Val 
            100                 105                 110         
Ile Ser Ala His Arg Gly Leu Pro Ser Ala Glu Met Phe Thr Asn Leu 
        115                 120                 125             
Asn Leu Val Lys Lys Gly Asp Thr Phe Tyr Phe Arg Val Leu Asn Lys 
    130                 135                 140                 
Val Leu Ala Tyr Lys Val Asp Gln Ile Leu Thr Val Glu Pro Asp Gln 
145                 150                 155                 160 
Val Thr Ser Leu Ser Gly Val Met Gly Lys Asp Tyr Ala Thr Leu Val 
                165                 170                 175     
Thr Cys Thr Pro Tyr Gly Val Asn Thr Lys Arg Leu Leu Val Arg Gly 
            180                 185                 190         
His Arg Ile Ala Tyr His Tyr Lys Lys Tyr Gln Gln Ala Lys Lys Ala 
        195                 200                 205             
Met Lys Leu Val Asp Lys Ser Arg Met Trp Ala Glu Val Val Cys Ala 
    210                 215                 220                 
Ala Phe Gly Val Val Ile Ala Ile Ile Leu Val Phe Met Tyr Ser Arg 
225                 230                 235                 240 
Val Ser Ala Lys Lys Ser Lys 
                245         

<210> 64
<211> 273
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS sortase C1 of PI-1 substitution mutant

<400> 64
Ile Val Ser Gln Val Met Tyr Phe Gln Ala Ser His Ala Asn Ile Asn 
1               5                   10                  15      
Ala Phe Lys Glu Ala Val Thr Lys Ile Asp Arg Val Glu Ile Asn Arg 
            20                  25                  30          
Arg Leu Glu Leu Ala Tyr Ala Tyr Asn Ala Ser Ile Ala Gly Ala Lys 
        35                  40                  45              
Thr Asn Gly Glu Tyr Pro Ala Leu Lys Ala Ala Ala Ser Ala Glu Gln 
    50                  55                  60                  
Lys Gln Ala Gly Val Val Glu Tyr Ala Arg Met Leu Glu Val Lys Glu 
65                  70                  75                  80  
Gln Ile Gly His Val Ile Ile Pro Arg Ile Asn Gln Asp Ile Pro Ile 
                85                  90                  95      
Tyr Ala Gly Ser Ala Glu Glu Asn Leu Gln Arg Gly Val Gly His Leu 
            100                 105                 110         
Glu Gly Thr Ser Leu Pro Val Gly Gly Glu Ser Thr His Ala Val Leu 
        115                 120                 125             
Thr Ala His Arg Gly Leu Pro Thr Ala Lys Leu Phe Thr Asn Leu Asp 
    130                 135                 140                 
Lys Val Thr Val Gly Asp Arg Phe Tyr Ile Glu His Ile Gly Gly Lys 
145                 150                 155                 160 
Ile Ala Tyr Gln Val Asp Gln Ile Lys Val Ile Ala Pro Asp Gln Leu 
                165                 170                 175     
Glu Asp Leu Tyr Val Ile Gln Gly Glu Asp His Val Thr Leu Leu Thr 
            180                 185                 190         
Cys Thr Pro Tyr Met Ile Asn Ser His Arg Leu Leu Val Arg Gly Lys 
        195                 200                 205             
Arg Ile Pro Tyr Val Glu Lys Thr Val Gln Lys Asp Ser Lys Thr Phe 
    210                 215                 220                 
Arg Gln Gln Gln Tyr Leu Thr Tyr Ala Met Trp Val Val Val Gly Leu 
225                 230                 235                 240 
Ile Leu Leu Ser Leu Leu Ile Trp Phe Lys Lys Thr Lys Gln Lys Lys 
                245                 250                 255     
Arg Arg Lys Asn Glu Lys Ala Ala Ser Gln Asn Ser His Asn Asn Ser 
            260                 265                 270         
Lys 
    

<210> 65
<211> 242
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS sortase C2 of PI-1 substitution mutant

<400> 65
Ala Ser His Gln Asn Ile Asn Gln Phe Lys Arg Glu Val Ala Lys Ile 
1               5                   10                  15      
Asp Thr Asn Thr Val Glu Arg Arg Ile Ala Leu Ala Asn Ala Tyr Asn 
            20                  25                  30          
Glu Thr Leu Ser Arg Asn Pro Leu Leu Ile Ala Ala Ala Thr Ser Lys 
        35                  40                  45              
Gln Lys Glu Gly Leu Arg Glu Tyr Ala Arg Met Leu Glu Val His Glu 
    50                  55                  60                  
Gln Ile Gly His Val Ala Ile Pro Ser Ile Gly Val Asp Ile Pro Ile 
65                  70                  75                  80  
Tyr Ala Gly Thr Ser Glu Thr Val Leu Gln Lys Gly Ser Gly His Leu 
                85                  90                  95      
Glu Gly Thr Ser Leu Pro Val Gly Gly Leu Ser Thr His Ser Val Leu 
            100                 105                 110         
Thr Ala His Arg Gly Leu Pro Thr Ala Arg Leu Phe Thr Asp Leu Asn 
        115                 120                 125             
Lys Val Lys Lys Gly Gln Ile Phe Tyr Val Thr Asn Ile Lys Glu Thr 
    130                 135                 140                 
Leu Ala Tyr Lys Val Val Ser Ile Lys Val Val Asp Pro Thr Ala Leu 
145                 150                 155                 160 
Ser Glu Val Lys Ile Val Asn Gly Lys Asp Tyr Ile Thr Leu Leu Thr 
                165                 170                 175     
Cys Thr Pro Tyr Met Ile Asn Ser His Arg Leu Leu Val Lys Gly Glu 
            180                 185                 190         
Arg Ile Pro Tyr Asp Ser Thr Glu Ala Glu Lys His Lys Glu Gln Thr 
        195                 200                 205             
Val Gln Asp Tyr Arg Leu Ser Leu Val Leu Lys Ile Leu Leu Val Leu 
    210                 215                 220                 
Leu Ile Gly Leu Phe Ile Val Ile Met Met Arg Arg Trp Met Gln His 
225                 230                 235                 240 
Arg Gln 
        

<210> 66
<211> 250
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS sortase C1 of PI-2a substitution mutant

<400> 66
Glu Ser Asn Asn Gln Thr Gln Asp Phe Glu Arg Ala Ala Lys Lys Leu 
1               5                   10                  15      
Ser Gln Lys Glu Ile Asn Arg Arg Met Ala Leu Ala Gln Ala Tyr Asn 
            20                  25                  30          
Asp Ser Leu Asn Asn Val His Leu Glu Ala Ala Ala Glu Lys Lys Arg 
        35                  40                  45              
Ile Gln Lys Gly Ile Ala Glu Tyr Ala Arg Met Leu Glu Val Ser Glu 
    50                  55                  60                  
Lys Ile Gly Ile Ile Ser Val Pro Lys Ile Gly Gln Lys Leu Pro Ile 
65                  70                  75                  80  
Phe Ala Gly Ser Ser Gln Glu Val Leu Ser Lys Gly Ala Gly His Leu 
                85                  90                  95      
Glu Gly Thr Ser Leu Pro Ile Gly Gly Asn Ser Thr His Thr Val Ile 
            100                 105                 110         
Thr Ala His Ser Gly Ile Pro Asp Lys Glu Leu Phe Ser Asn Leu Lys 
        115                 120                 125             
Lys Leu Lys Lys Gly Asp Lys Phe Tyr Ile Gln Asn Ile Lys Glu Thr 
    130                 135                 140                 
Ile Ala Tyr Gln Val Asp Gln Ile Lys Val Val Thr Pro Asp Asn Phe 
145                 150                 155                 160 
Ser Asp Leu Leu Val Val Pro Gly His Asp Tyr Ala Thr Leu Leu Thr 
                165                 170                 175     
Cys Thr Pro Ile Met Val Asn Thr His Arg Leu Leu Val Arg Gly His 
            180                 185                 190         
Arg Ile Pro Tyr Lys Gly Pro Ile Asp Glu Lys Leu Ile Lys Asp Gly 
        195                 200                 205             
His Leu Asn Thr Ile Tyr Arg Tyr Leu Phe Tyr Ile Ser Leu Val Ile 
    210                 215                 220                 
Ile Ala Trp Leu Leu Trp Leu Ile Lys Arg Gln Arg Gln Lys Asn Arg 
225                 230                 235                 240 
Leu Ser Ser Val Arg Lys Gly Ile Glu Ser 
                245                 250 

<210> 67
<211> 248
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS sortase C2 of PI-2a substitution mutant

<400> 67
Lys Ala Asn Asn Gln Val Thr Asn Phe Asp Asn Gln Thr Gln Lys Leu 
1               5                   10                  15      
Asn Ala Lys Glu Ile Asn Arg Arg Phe Glu Leu Ala Lys Ala Tyr Asn 
            20                  25                  30          
Arg Thr Leu Asp Pro Ser Arg Leu Ser Ala Ala Ala Thr Glu Lys Glu 
        35                  40                  45              
Lys Lys Gly Ile Ala Glu Tyr Ala His Met Leu Glu Ile Thr Glu Met 
    50                  55                  60                  
Ile Gly Tyr Ile Asp Ile Pro Ser Ile Lys Gln Lys Leu Pro Ile Tyr 
65                  70                  75                  80  
Ala Gly Thr Thr Ser Ser Val Leu Glu Lys Gly Ser Gly His Leu Glu 
                85                  90                  95      
Gly Thr Ser Leu Pro Ile Gly Gly Lys Ser Ser His Thr Val Ile Thr 
            100                 105                 110         
Ala His Arg Gly Leu Pro Lys Ala Lys Leu Phe Thr Asp Leu Asp Lys 
        115                 120                 125             
Leu Lys Lys Gly Lys Ile Phe Tyr Ile His Asn Ile Lys Glu Val Leu 
    130                 135                 140                 
Ala Tyr Lys Val Asp Gln Ile Ser Val Val Lys Pro Asp Asn Phe Ser 
145                 150                 155                 160 
Lys Leu Leu Val Val Lys Gly Lys Asp Tyr Ala Thr Leu Leu Thr Cys 
                165                 170                 175     
Thr Pro Tyr Ser Ile Asn Ser His Arg Leu Leu Val Arg Gly His Arg 
            180                 185                 190         
Ile Lys Tyr Val Pro Pro Val Lys Glu Lys Asn Tyr Leu Met Lys Glu 
        195                 200                 205             
Leu Gln Thr His Tyr Lys Leu Tyr Phe Leu Leu Ser Ile Leu Val Ile 
    210                 215                 220                 
Leu Ile Leu Val Ala Leu Leu Leu Tyr Leu Lys Arg Lys Phe Lys Glu 
225                 230                 235                 240 
Arg Lys Arg Lys Gly Asn Gln Lys 
                245             

<210> 68
<211> 255
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS sortase C1 of PI-2b substitution mutant

<400> 68
His Gln Ser Arg Ala Ile Met Asp Tyr Gln Asp Arg Val Thr His Met 
1               5                   10                  15      
Asp Glu Asn Asp Tyr Lys Lys Ile Ile Asn Arg Ala Lys Glu Tyr Asn 
            20                  25                  30          
Lys Gln Phe Lys Thr Ser Gly Ala Ala Ala His Met Thr Ser Gln Glu 
        35                  40                  45              
Arg Leu Asp Tyr Asn Ser Gln Leu Ala Ile Asp Lys Thr Gly Asn Met 
    50                  55                  60                  
Gly Tyr Ile Ser Ile Pro Lys Ile Asn Ile Lys Leu Pro Leu Tyr His 
65                  70                  75                  80  
Gly Thr Ser Glu Lys Val Leu Gln Thr Ser Ile Gly His Leu Glu Gly 
                85                  90                  95      
Ser Ser Leu Pro Ile Gly Gly Asp Ser Thr His Ser Ile Leu Ser Gly 
            100                 105                 110         
His Arg Gly Leu Pro Ser Ser Arg Leu Phe Ser Asp Leu Asp Lys Leu 
        115                 120                 125             
Lys Val Gly Asp His Trp Thr Val Ser Ile Leu Asn Glu Thr Tyr Thr 
    130                 135                 140                 
Tyr Gln Val Asp Gln Ile Arg Thr Val Lys Pro Asp Asp Leu Arg Asp 
145                 150                 155                 160 
Leu Gln Ile Val Lys Gly Lys Asp Tyr Gln Thr Leu Val Thr Cys Thr 
                165                 170                 175     
Pro Tyr Gly Val Asn Thr His Arg Leu Leu Val Arg Gly His Arg Val 
            180                 185                 190         
Pro Asn Asp Asn Gly Asn Ala Leu Val Val Ala Glu Ala Ile Gln Ile 
        195                 200                 205             
Glu Pro Ile Tyr Ile Ala Pro Phe Ile Ala Ile Phe Leu Thr Leu Ile 
    210                 215                 220                 
Leu Leu Leu Ile Ser Leu Glu Val Thr Arg Arg Ala Arg Gln Arg Lys 
225                 230                 235                 240 
Lys Ile Leu Lys Gln Ala Met Arg Lys Glu Glu Asn Asn Asp Leu 
                245                 250                 255 

<210> 69
<211> 263
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C1 substitution mutant

<400> 69
Glu Ser Asn Gln Gln Ile Ala Asp Phe Asp Lys Glu Lys Ala Thr Leu 
1               5                   10                  15      
Asp Glu Ala Asp Ile Asp Glu Arg Met Lys Leu Ala Gln Ala Phe Asn 
            20                  25                  30          
Asp Ser Leu Asn Asn Val Val Ser Gly Ala Ala Ala Ser Glu Glu Met 
        35                  40                  45              
Lys Lys Lys Gly Arg Ala Glu Tyr Ala Arg Met Leu Glu Ile His Glu 
    50                  55                  60                  
Arg Met Gly His Val Glu Ile Pro Val Ile Asp Val Asp Leu Pro Val 
65                  70                  75                  80  
Tyr Ala Gly Thr Ala Glu Glu Val Leu Gln Gln Gly Ala Gly His Leu 
                85                  90                  95      
Glu Gly Thr Ser Leu Pro Ile Gly Gly Asn Ser Thr His Ala Val Ile 
            100                 105                 110         
Thr Ala His Thr Gly Leu Pro Thr Ala Lys Met Phe Thr Asp Leu Thr 
        115                 120                 125             
Lys Leu Lys Val Gly Asp Lys Phe Tyr Val His Asn Ile Lys Glu Val 
    130                 135                 140                 
Met Ala Tyr Gln Val Asp Gln Val Lys Val Ile Glu Pro Thr Asn Phe 
145                 150                 155                 160 
Asp Asp Leu Leu Ile Val Pro Gly His Asp Tyr Val Thr Leu Leu Thr 
                165                 170                 175     
Cys Thr Pro Tyr Met Ile Asn Thr His Arg Leu Leu Val Arg Gly His 
            180                 185                 190         
Arg Ile Pro Tyr Val Ala Glu Val Glu Glu Glu Phe Ile Ala Ala Asn 
        195                 200                 205             
Lys Leu Ser His Leu Tyr Arg Tyr Leu Phe Tyr Val Ala Val Gly Leu 
    210                 215                 220                 
Ile Val Ile Leu Leu Trp Ile Ile Arg Arg Leu Arg Lys Lys Lys Lys 
225                 230                 235                 240 
Gln Pro Glu Lys Ala Leu Lys Ala Leu Lys Ala Ala Arg Lys Glu Val 
                245                 250                 255     
Lys Val Glu Asp Gly Gln Gln 
            260             

<210> 70
<211> 253
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C2 substitution mutant

<400> 70
Glu Ser Asn Glu Val Ile Lys Glu Phe Asp Glu Thr Val Ser Gln Met 
1               5                   10                  15      
Asp Lys Ala Glu Leu Glu Glu Arg Trp Arg Leu Ala Gln Ala Phe Asn 
            20                  25                  30          
Ala Thr Leu Lys Pro Ser Glu Ile Leu Ala Ala Ala Thr Glu Gln Glu 
        35                  40                  45              
Lys Lys Lys Gly Val Ser Glu Tyr Ala Asn Met Leu Lys Val His Glu 
    50                  55                  60                  
Arg Ile Gly Tyr Val Glu Ile Pro Ala Ile Asp Gln Glu Ile Pro Met 
65                  70                  75                  80  
Tyr Val Gly Thr Ser Glu Asp Ile Leu Gln Lys Gly Ala Gly Leu Leu 
                85                  90                  95      
Glu Gly Ala Ser Leu Pro Val Gly Gly Glu Asn Thr His Thr Val Ile 
            100                 105                 110         
Thr Ala His Arg Gly Leu Pro Thr Ala Glu Leu Phe Ser Gln Leu Asp 
        115                 120                 125             
Lys Met Lys Lys Gly Asp Ile Phe Tyr Leu His Val Leu Asp Gln Val 
    130                 135                 140                 
Leu Ala Tyr Gln Val Asp Gln Ile Val Thr Val Glu Pro Asn Asp Phe 
145                 150                 155                 160 
Glu Pro Val Leu Ile Gln His Gly Glu Asp Tyr Ala Thr Leu Leu Thr 
                165                 170                 175     
Cys Thr Pro Tyr Met Ile Asn Ser His Arg Leu Leu Val Arg Gly Lys 
            180                 185                 190         
Arg Ile Pro Tyr Thr Ala Pro Ile Ala Glu Arg Asn Arg Ala Val Arg 
        195                 200                 205             
Glu Arg Gly Gln Phe Trp Leu Trp Leu Leu Leu Gly Ala Met Ala Val 
    210                 215                 220                 
Ile Leu Leu Leu Leu Tyr Arg Val Tyr Arg Asn Arg Arg Ile Val Lys 
225                 230                 235                 240 
Gly Leu Glu Lys Gln Leu Glu Gly Arg His Val Lys Asp 
                245                 250             

<210> 71
<211> 252
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> Pneumococcus sortase C3 substitution mutant

<400> 71
Gln Ser Leu Gly Gln Val Lys Gly His Ala Thr Phe Val Lys Ser Met 
1               5                   10                  15      
Thr Thr Glu Met Tyr Gln Glu Gln Gln Asn His Ser Leu Ala Tyr Asn 
            20                  25                  30          
Gln Arg Leu Ala Ser Gln Asn Arg Ile Val Ala Ala Ala Leu Ala Glu 
        35                  40                  45              
Gly Tyr Glu Val Asn Tyr Gln Val Ser Asp Asp Pro Asp Ala Val Tyr 
    50                  55                  60                  
Gly Tyr Leu Ser Ile Pro Ser Leu Glu Ile Met Glu Pro Val Tyr Leu 
65                  70                  75                  80  
Gly Ala Asp Tyr His His Leu Gly Met Gly Leu Ala His Val Asp Gly 
                85                  90                  95      
Thr Pro Leu Pro Leu Asp Gly Thr Gly Ile Arg Ser Val Ile Ala Gly 
            100                 105                 110         
His Arg Ala Glu Pro Ser His Val Phe Phe Arg His Leu Asp Gln Leu 
        115                 120                 125             
Lys Val Gly Asp Ala Leu Tyr Tyr Asp Asn Gly Gln Glu Ile Val Glu 
    130                 135                 140                 
Tyr Gln Met Met Asp Thr Glu Ile Ile Leu Pro Ser Glu Trp Glu Lys 
145                 150                 155                 160 
Leu Glu Ser Val Ser Ser Lys Asn Ile Met Thr Leu Ile Thr Cys Asp 
                165                 170                 175     
Pro Ile Pro Thr Phe Asn Lys Arg Leu Leu Val Asn Phe Glu Arg Val 
            180                 185                 190         
Ala Val Tyr Gln Lys Ser Asp Pro Gln Thr Ala Ala Val Ala Arg Val 
        195                 200                 205             
Ala Phe Thr Lys Glu Gly Gln Ser Val Ser Arg Val Ala Thr Ser Gln 
    210                 215                 220                 
Trp Leu Tyr Arg Gly Leu Val Val Leu Ala Phe Leu Gly Ile Leu Phe 
225                 230                 235                 240 
Val Leu Trp Lys Leu Ala Arg Leu Leu Arg Gly Lys 
                245                 250         

<210> 72
<211> 554
<212> PRT
<213> Streptococcus agalactiae 2603V/R

<220> 
<223> GBS80 full length

<400> 72
Met Lys Leu Ser Lys Lys Leu Leu Phe Ser Ala Ala Val Leu Thr Met 
1               5                   10                  15      
Val Ala Gly Ser Thr Val Glu Pro Val Ala Gln Phe Ala Thr Gly Met 
            20                  25                  30          
Ser Ile Val Arg Ala Ala Glu Val Ser Gln Glu Arg Pro Ala Lys Thr 
        35                  40                  45              
Thr Val Asn Ile Tyr Lys Leu Gln Ala Asp Ser Tyr Lys Ser Glu Ile 
    50                  55                  60                  
Thr Ser Asn Gly Gly Ile Glu Asn Lys Asp Gly Glu Val Ile Ser Asn 
65                  70                  75                  80  
Tyr Ala Lys Leu Gly Asp Asn Val Lys Gly Leu Gln Gly Val Gln Phe 
                85                  90                  95      
Lys Arg Tyr Lys Val Lys Thr Asp Ile Ser Val Asp Glu Leu Lys Lys 
            100                 105                 110         
Leu Thr Thr Val Glu Ala Ala Asp Ala Lys Val Gly Thr Ile Leu Glu 
        115                 120                 125             
Glu Gly Val Ser Leu Pro Gln Lys Thr Asn Ala Gln Gly Leu Val Val 
    130                 135                 140                 
Asp Ala Leu Asp Ser Lys Ser Asn Val Arg Tyr Leu Tyr Val Glu Asp 
145                 150                 155                 160 
Leu Lys Asn Ser Pro Ser Asn Ile Thr Lys Ala Tyr Ala Val Pro Phe 
                165                 170                 175     
Val Leu Glu Leu Pro Val Ala Asn Ser Thr Gly Thr Gly Phe Leu Ser 
            180                 185                 190         
Glu Ile Asn Ile Tyr Pro Lys Asn Val Val Thr Asp Glu Pro Lys Thr 
        195                 200                 205             
Asp Lys Asp Val Lys Lys Leu Gly Gln Asp Asp Ala Gly Tyr Thr Ile 
    210                 215                 220                 
Gly Glu Glu Phe Lys Trp Phe Leu Lys Ser Thr Ile Pro Ala Asn Leu 
225                 230                 235                 240 
Gly Asp Tyr Glu Lys Phe Glu Ile Thr Asp Lys Phe Ala Asp Gly Leu 
                245                 250                 255     
Thr Tyr Lys Ser Val Gly Lys Ile Lys Ile Gly Ser Lys Thr Leu Asn 
            260                 265                 270         
Arg Asp Glu His Tyr Thr Ile Asp Glu Pro Thr Val Asp Asn Gln Asn 
        275                 280                 285             
Thr Leu Lys Ile Thr Phe Lys Pro Glu Lys Phe Lys Glu Ile Ala Glu 
    290                 295                 300                 
Leu Leu Lys Gly Met Thr Leu Val Lys Asn Gln Asp Ala Leu Asp Lys 
305                 310                 315                 320 
Ala Thr Ala Asn Thr Asp Asp Ala Ala Phe Leu Glu Ile Pro Val Ala 
                325                 330                 335     
Ser Thr Ile Asn Glu Lys Ala Val Leu Gly Lys Ala Ile Glu Asn Thr 
            340                 345                 350         
Phe Glu Leu Gln Tyr Asp His Thr Pro Asp Lys Ala Asp Asn Pro Lys 
        355                 360                 365             
Pro Ser Asn Pro Pro Arg Lys Pro Glu Val His Thr Gly Gly Lys Arg 
    370                 375                 380                 
Phe Val Lys Lys Asp Ser Thr Glu Thr Gln Thr Leu Gly Gly Ala Glu 
385                 390                 395                 400 
Phe Asp Leu Leu Ala Ser Asp Gly Thr Ala Val Lys Trp Thr Asp Ala 
                405                 410                 415     
Leu Ile Lys Ala Asn Thr Asn Lys Asn Tyr Ile Ala Gly Glu Ala Val 
            420                 425                 430         
Thr Gly Gln Pro Ile Lys Leu Lys Ser His Thr Asp Gly Thr Phe Glu 
        435                 440                 445             
Ile Lys Gly Leu Ala Tyr Ala Val Asp Ala Asn Ala Glu Gly Thr Ala 
    450                 455                 460                 
Val Thr Tyr Lys Leu Lys Glu Thr Lys Ala Pro Glu Gly Tyr Val Ile 
465                 470                 475                 480 
Pro Asp Lys Glu Ile Glu Phe Thr Val Ser Gln Thr Ser Tyr Asn Thr 
                485                 490                 495     
Lys Pro Thr Asp Ile Thr Val Asp Ser Ala Asp Ala Thr Pro Asp Thr 
            500                 505                 510         
Ile Lys Asn Asn Lys Arg Pro Ser Ile Pro Asn Thr Gly Gly Ile Gly 
        515                 520                 525             
Thr Ala Ile Phe Val Ala Ile Gly Ala Ala Val Met Ala Phe Ala Val 
    530                 535                 540                 
Lys Gly Met Lys Arg Arg Thr Lys Asp Asn 
545                 550                 

<210> 73
<211> 517
<212> PRT
<213> Streptococcus agalactiae 2603V/R

<220> 
<223> GBS80 Leader/Signal sequence removed

<400> 73
Ala Glu Val Ser Gln Glu Arg Pro Ala Lys Thr Thr Val Asn Ile Tyr 
1               5                   10                  15      
Lys Leu Gln Ala Asp Ser Tyr Lys Ser Glu Ile Thr Ser Asn Gly Gly 
            20                  25                  30          
Ile Glu Asn Lys Asp Gly Glu Val Ile Ser Asn Tyr Ala Lys Leu Gly 
        35                  40                  45              
Asp Asn Val Lys Gly Leu Gln Gly Val Gln Phe Lys Arg Tyr Lys Val 
    50                  55                  60                  
Lys Thr Asp Ile Ser Val Asp Glu Leu Lys Lys Leu Thr Thr Val Glu 
65                  70                  75                  80  
Ala Ala Asp Ala Lys Val Gly Thr Ile Leu Glu Glu Gly Val Ser Leu 
                85                  90                  95      
Pro Gln Lys Thr Asn Ala Gln Gly Leu Val Val Asp Ala Leu Asp Ser 
            100                 105                 110         
Lys Ser Asn Val Arg Tyr Leu Tyr Val Glu Asp Leu Lys Asn Ser Pro 
        115                 120                 125             
Ser Asn Ile Thr Lys Ala Tyr Ala Val Pro Phe Val Leu Glu Leu Pro 
    130                 135                 140                 
Val Ala Asn Ser Thr Gly Thr Gly Phe Leu Ser Glu Ile Asn Ile Tyr 
145                 150                 155                 160 
Pro Lys Asn Val Val Thr Asp Glu Pro Lys Thr Asp Lys Asp Val Lys 
                165                 170                 175     
Lys Leu Gly Gln Asp Asp Ala Gly Tyr Thr Ile Gly Glu Glu Phe Lys 
            180                 185                 190         
Trp Phe Leu Lys Ser Thr Ile Pro Ala Asn Leu Gly Asp Tyr Glu Lys 
        195                 200                 205             
Phe Glu Ile Thr Asp Lys Phe Ala Asp Gly Leu Thr Tyr Lys Ser Val 
    210                 215                 220                 
Gly Lys Ile Lys Ile Gly Ser Lys Thr Leu Asn Arg Asp Glu His Tyr 
225                 230                 235                 240 
Thr Ile Asp Glu Pro Thr Val Asp Asn Gln Asn Thr Leu Lys Ile Thr 
                245                 250                 255     
Phe Lys Pro Glu Lys Phe Lys Glu Ile Ala Glu Leu Leu Lys Gly Met 
            260                 265                 270         
Thr Leu Val Lys Asn Gln Asp Ala Leu Asp Lys Ala Thr Ala Asn Thr 
        275                 280                 285             
Asp Asp Ala Ala Phe Leu Glu Ile Pro Val Ala Ser Thr Ile Asn Glu 
    290                 295                 300                 
Lys Ala Val Leu Gly Lys Ala Ile Glu Asn Thr Phe Glu Leu Gln Tyr 
305                 310                 315                 320 
Asp His Thr Pro Asp Lys Ala Asp Asn Pro Lys Pro Ser Asn Pro Pro 
                325                 330                 335     
Arg Lys Pro Glu Val His Thr Gly Gly Lys Arg Phe Val Lys Lys Asp 
            340                 345                 350         
Ser Thr Glu Thr Gln Thr Leu Gly Gly Ala Glu Phe Asp Leu Leu Ala 
        355                 360                 365             
Ser Asp Gly Thr Ala Val Lys Trp Thr Asp Ala Leu Ile Lys Ala Asn 
    370                 375                 380                 
Thr Asn Lys Asn Tyr Ile Ala Gly Glu Ala Val Thr Gly Gln Pro Ile 
385                 390                 395                 400 
Lys Leu Lys Ser His Thr Asp Gly Thr Phe Glu Ile Lys Gly Leu Ala 
                405                 410                 415     
Tyr Ala Val Asp Ala Asn Ala Glu Gly Thr Ala Val Thr Tyr Lys Leu 
            420                 425                 430         
Lys Glu Thr Lys Ala Pro Glu Gly Tyr Val Ile Pro Asp Lys Glu Ile 
        435                 440                 445             
Glu Phe Thr Val Ser Gln Thr Ser Tyr Asn Thr Lys Pro Thr Asp Ile 
    450                 455                 460                 
Thr Val Asp Ser Ala Asp Ala Thr Pro Asp Thr Ile Lys Asn Asn Lys 
465                 470                 475                 480 
Arg Pro Ser Ile Pro Asn Thr Gly Gly Ile Gly Thr Ala Ile Phe Val 
                485                 490                 495     
Ala Ile Gly Ala Ala Val Met Ala Phe Ala Val Lys Gly Met Lys Arg 
            500                 505                 510         
Arg Thr Lys Asp Asn 
        515         

<210> 74
<211> 705
<212> PRT
<213> Streptococcus agalactiae 2603V/R

<220> 
<223> GBS59 (BP from GBS PI-2a) full length

<400> 74
Met Lys Arg Ile Asn Lys Tyr Phe Ala Met Phe Ser Ala Leu Leu Leu 
1               5                   10                  15      
Thr Leu Thr Ser Leu Leu Ser Val Ala Pro Ala Phe Ala Asp Glu Ala 
            20                  25                  30          
Thr Thr Asn Thr Val Thr Leu His Lys Ile Leu Gln Thr Glu Ser Asn 
        35                  40                  45              
Leu Asn Lys Ser Asn Phe Pro Gly Thr Thr Gly Leu Asn Gly Lys Asp 
    50                  55                  60                  
Tyr Lys Gly Gly Ala Ile Ser Asp Leu Ala Gly Tyr Phe Gly Glu Gly 
65                  70                  75                  80  
Ser Lys Glu Ile Glu Gly Ala Phe Phe Ala Leu Ala Leu Lys Glu Asp 
                85                  90                  95      
Lys Ser Gly Lys Val Gln Tyr Val Lys Ala Lys Glu Gly Asn Lys Leu 
            100                 105                 110         
Thr Pro Ala Leu Ile Asn Lys Asp Gly Thr Pro Glu Ile Thr Val Asn 
        115                 120                 125             
Ile Asp Glu Ala Val Ser Gly Leu Thr Pro Glu Gly Asp Thr Gly Leu 
    130                 135                 140                 
Val Phe Asn Thr Lys Gly Leu Lys Gly Glu Phe Lys Ile Val Glu Val 
145                 150                 155                 160 
Lys Ser Lys Ser Thr Tyr Asn Asn Asn Gly Ser Leu Leu Ala Ala Ser 
                165                 170                 175     
Lys Ala Val Pro Val Asn Ile Thr Leu Pro Leu Val Asn Glu Asp Gly 
            180                 185                 190         
Val Val Ala Asp Ala His Val Tyr Pro Lys Asn Thr Glu Glu Lys Pro 
        195                 200                 205             
Glu Ile Asp Lys Asn Phe Ala Lys Thr Asn Asp Leu Thr Ala Leu Thr 
    210                 215                 220                 
Asp Val Asn Arg Leu Leu Thr Ala Gly Ala Asn Tyr Gly Asn Tyr Ala 
225                 230                 235                 240 
Arg Asp Lys Ala Thr Ala Thr Ala Glu Ile Gly Lys Val Val Pro Tyr 
                245                 250                 255     
Glu Val Lys Thr Lys Ile His Lys Gly Ser Lys Tyr Glu Asn Leu Val 
            260                 265                 270         
Trp Thr Asp Ile Met Ser Asn Gly Leu Thr Met Gly Ser Thr Val Ser 
        275                 280                 285             
Leu Lys Ala Ser Gly Thr Thr Glu Thr Phe Ala Lys Asp Thr Asp Tyr 
    290                 295                 300                 
Glu Leu Ser Ile Asp Ala Arg Gly Phe Thr Leu Lys Phe Thr Ala Asp 
305                 310                 315                 320 
Gly Leu Gly Lys Leu Glu Lys Ala Ala Lys Thr Ala Asp Ile Glu Phe 
                325                 330                 335     
Thr Leu Thr Tyr Ser Ala Thr Val Asn Gly Gln Ala Ile Ile Asp Asn 
            340                 345                 350         
Pro Glu Ser Asn Asp Ile Lys Leu Ser Tyr Gly Asn Lys Pro Gly Lys 
        355                 360                 365             
Asp Leu Thr Glu Leu Pro Val Thr Pro Ser Lys Gly Glu Val Thr Val 
    370                 375                 380                 
Ala Lys Thr Trp Ser Asp Gly Ile Ala Pro Asp Gly Val Asn Val Val 
385                 390                 395                 400 
Tyr Thr Leu Lys Asp Lys Asp Lys Thr Val Ala Ser Val Ser Leu Thr 
                405                 410                 415     
Lys Thr Ser Lys Gly Thr Ile Asp Leu Gly Asn Gly Ile Lys Phe Glu 
            420                 425                 430         
Val Ser Gly Asn Phe Ser Gly Lys Phe Thr Gly Leu Glu Asn Lys Ser 
        435                 440                 445             
Tyr Met Ile Ser Glu Arg Val Ser Gly Tyr Gly Ser Ala Ile Asn Leu 
    450                 455                 460                 
Glu Asn Gly Lys Val Thr Ile Thr Asn Thr Lys Asp Ser Asp Asn Pro 
465                 470                 475                 480 
Thr Pro Leu Asn Pro Thr Glu Pro Lys Val Glu Thr His Gly Lys Lys 
                485                 490                 495     
Phe Val Lys Thr Asn Glu Gln Gly Asp Arg Leu Ala Gly Ala Gln Phe 
            500                 505                 510         
Val Val Lys Asn Ser Ala Gly Lys Tyr Leu Ala Leu Lys Ala Asp Gln 
        515                 520                 525             
Ser Glu Gly Gln Lys Thr Leu Ala Ala Lys Lys Ile Ala Leu Asp Glu 
    530                 535                 540                 
Ala Ile Ala Ala Tyr Asn Lys Leu Ser Ala Thr Asp Gln Lys Gly Glu 
545                 550                 555                 560 
Lys Gly Ile Thr Ala Lys Glu Leu Ile Lys Thr Lys Gln Ala Asp Tyr 
                565                 570                 575     
Asp Ala Ala Phe Ile Glu Ala Arg Thr Ala Tyr Glu Trp Ile Thr Asp 
            580                 585                 590         
Lys Ala Arg Ala Ile Thr Tyr Thr Ser Asn Asp Gln Gly Gln Phe Glu 
        595                 600                 605             
Val Thr Gly Leu Ala Asp Gly Thr Tyr Asn Leu Glu Glu Thr Leu Ala 
    610                 615                 620                 
Pro Ala Gly Phe Ala Lys Leu Ala Gly Asn Ile Lys Phe Val Val Asn 
625                 630                 635                 640 
Gln Gly Ser Tyr Ile Thr Gly Gly Asn Ile Asp Tyr Val Ala Asn Ser 
                645                 650                 655     
Asn Gln Lys Asp Ala Thr Arg Val Glu Asn Lys Lys Val Thr Ile Pro 
            660                 665                 670         
Gln Thr Gly Gly Ile Gly Thr Ile Leu Phe Thr Ile Ile Gly Leu Ser 
        675                 680                 685             
Ile Met Leu Gly Ala Val Val Ile Met Lys Arg Arg Gln Ser Lys Glu 
    690                 695                 700                 
Ala 
705 

<210> 75
<211> 675
<212> PRT
<213> Streptococcus agalactiae H36B

<220> 
<223> GBS59

<400> 75
Met Lys Lys Ile Asn Lys Tyr Phe Ala Val Phe Ser Ala Leu Leu Leu 
1               5                   10                  15      
Thr Val Thr Ser Leu Phe Ser Val Ala Pro Val Phe Ala Glu Glu Ala 
            20                  25                  30          
Lys Thr Thr Asp Thr Val Thr Leu His Lys Ile Val Met Pro Arg Thr 
        35                  40                  45              
Ala Phe Asp Gly Phe Thr Ala Gly Thr Lys Gly Lys Asp Asn Thr Asp 
    50                  55                  60                  
Tyr Val Gly Lys Gln Ile Glu Asp Leu Lys Thr Tyr Phe Gly Ser Gly 
65                  70                  75                  80  
Glu Ala Lys Glu Ile Ala Gly Ala Tyr Phe Ala Phe Lys Asn Glu Ala 
                85                  90                  95      
Gly Thr Lys Tyr Ile Thr Glu Asn Gly Glu Glu Val Asp Thr Leu Asp 
            100                 105                 110         
Thr Thr Asp Ala Lys Gly Cys Ala Val Leu Lys Gly Leu Thr Thr Asp 
        115                 120                 125             
Asn Gly Phe Lys Phe Asn Thr Ser Lys Leu Thr Gly Thr Tyr Gln Ile 
    130                 135                 140                 
Val Glu Leu Lys Glu Lys Ser Thr Tyr Asn Asn Asp Gly Ser Ile Leu 
145                 150                 155                 160 
Ala Asp Ser Lys Ala Val Pro Val Lys Ile Thr Leu Pro Leu Val Asn 
                165                 170                 175     
Asp Asn Gly Val Val Lys Asp Ala His Val Tyr Pro Lys Asn Thr Glu 
            180                 185                 190         
Thr Lys Pro Gln Val Asp Lys Asn Phe Ala Asp Lys Glu Leu Asp Tyr 
        195                 200                 205             
Ala Asn Asn Lys Lys Asp Lys Gly Thr Val Ser Ala Ser Val Gly Asp 
    210                 215                 220                 
Val Lys Lys Tyr His Val Gly Thr Lys Ile Leu Lys Gly Ser Asp Tyr 
225                 230                 235                 240 
Lys Lys Leu Ile Trp Thr Asp Ser Met Thr Lys Gly Leu Thr Phe Asn 
                245                 250                 255     
Asn Asp Ile Ala Val Thr Leu Asp Gly Ala Thr Leu Asp Ala Thr Asn 
            260                 265                 270         
Tyr Lys Leu Val Ala Asp Asp Gln Gly Phe Arg Leu Val Leu Thr Asp 
        275                 280                 285             
Lys Gly Leu Glu Ala Val Ala Lys Ala Ala Lys Thr Lys Asp Val Glu 
    290                 295                 300                 
Ile Lys Ile Thr Tyr Ser Ala Thr Leu Asn Gly Ser Ala Val Val Glu 
305                 310                 315                 320 
Val Leu Glu Thr Asn Asp Val Lys Leu Asp Tyr Gly Asn Asn Pro Thr 
                325                 330                 335     
Ile Glu Asn Glu Pro Lys Glu Gly Ile Pro Val Asp Lys Lys Ile Thr 
            340                 345                 350         
Val Asn Lys Thr Trp Ala Val Asp Gly Asn Glu Val Asn Lys Ala Asp 
        355                 360                 365             
Glu Thr Val Asp Ala Val Phe Thr Leu Gln Val Lys Asp Gly Asp Lys 
    370                 375                 380                 
Trp Val Asn Val Asp Ser Ala Lys Ala Thr Ala Ala Thr Ser Phe Lys 
385                 390                 395                 400 
His Thr Phe Glu Asn Leu Asp Asn Ala Lys Thr Tyr Arg Val Ile Glu 
                405                 410                 415     
Arg Val Ser Gly Tyr Ala Pro Glu Tyr Val Ser Phe Val Asn Gly Val 
            420                 425                 430         
Val Thr Ile Lys Asn Asn Lys Asp Ser Asn Glu Pro Thr Pro Ile Asn 
        435                 440                 445             
Pro Ser Glu Pro Lys Val Val Thr Tyr Gly Arg Lys Phe Val Lys Thr 
    450                 455                 460                 
Asn Lys Asp Gly Lys Glu Arg Leu Ala Gly Ala Thr Phe Leu Val Lys 
465                 470                 475                 480 
Lys Asp Gly Lys Tyr Leu Ala Arg Lys Ser Gly Val Ala Thr Asp Ala 
                485                 490                 495     
Glu Lys Ala Ala Val Asp Ser Thr Lys Ser Ala Leu Asp Ala Ala Val 
            500                 505                 510         
Lys Ala Tyr Asn Asp Leu Thr Lys Glu Lys Gln Glu Gly Gln Asp Gly 
        515                 520                 525             
Lys Ser Ala Leu Ala Thr Val Ser Glu Lys Gln Lys Ala Tyr Asn Asp 
    530                 535                 540                 
Ala Phe Val Lys Ala Asn Tyr Ser Tyr Glu Trp Val Glu Asp Lys Asn 
545                 550                 555                 560 
Ala Lys Asn Val Val Lys Leu Ile Ser Asn Asp Lys Gly Gln Phe Glu 
                565                 570                 575     
Ile Thr Gly Leu Thr Glu Gly Gln Tyr Ser Leu Glu Glu Thr Gln Ala 
            580                 585                 590         
Pro Thr Gly Tyr Ala Lys Leu Ser Gly Asp Val Ser Phe Asn Val Asn 
        595                 600                 605             
Ala Thr Ser Tyr Ser Lys Gly Ser Ala Gln Asp Ile Glu Tyr Thr Gln 
    610                 615                 620                 
Gly Ser Lys Thr Lys Asp Ala Gln Gln Val Ile Asn Lys Lys Val Thr 
625                 630                 635                 640 
Ile Pro Gln Thr Gly Gly Ile Gly Thr Ile Phe Phe Thr Ile Ile Gly 
                645                 650                 655     
Leu Ser Ile Met Leu Gly Ala Val Val Ile Met Lys Arg Arg Gln Ser 
            660                 665                 670         
Glu Glu Val 
        675 

<210> 76
<211> 674
<212> PRT
<213> Streptococcus agalactiae 515

<220> 
<223> GBS59

<400> 76
Met Lys Lys Ile Asn Lys Cys Leu Thr Met Phe Ser Thr Leu Leu Leu 
1               5                   10                  15      
Ile Leu Thr Ser Leu Phe Ser Val Ala Pro Ala Phe Ala Asp Asp Ala 
            20                  25                  30          
Thr Thr Asp Thr Val Thr Leu His Lys Ile Val Met Pro Gln Ala Ala 
        35                  40                  45              
Phe Asp Asn Phe Thr Glu Gly Thr Lys Gly Lys Asn Asp Ser Asp Tyr 
    50                  55                  60                  
Val Gly Lys Gln Ile Asn Asp Leu Lys Ser Tyr Phe Gly Ser Thr Asp 
65                  70                  75                  80  
Ala Lys Glu Ile Lys Gly Ala Phe Phe Val Phe Lys Asn Glu Thr Gly 
                85                  90                  95      
Thr Lys Phe Ile Thr Glu Asn Gly Lys Glu Val Asp Thr Leu Glu Ala 
            100                 105                 110         
Lys Asp Ala Glu Gly Gly Ala Val Leu Ser Gly Leu Thr Lys Asp Asn 
        115                 120                 125             
Gly Phe Val Phe Asn Thr Ala Lys Leu Lys Gly Ile Tyr Gln Ile Val 
    130                 135                 140                 
Glu Leu Lys Glu Lys Ser Asn Tyr Asp Asn Asn Gly Ser Ile Leu Ala 
145                 150                 155                 160 
Asp Ser Lys Ala Val Pro Val Lys Ile Thr Leu Pro Leu Val Asn Asn 
                165                 170                 175     
Gln Gly Val Val Lys Asp Ala His Ile Tyr Pro Lys Asn Thr Glu Thr 
            180                 185                 190         
Lys Pro Gln Val Asp Lys Asn Phe Ala Asp Lys Asp Leu Asp Tyr Thr 
        195                 200                 205             
Asp Asn Arg Lys Asp Lys Gly Val Val Ser Ala Thr Val Gly Asp Lys 
    210                 215                 220                 
Lys Glu Tyr Ile Val Gly Thr Lys Ile Leu Lys Gly Ser Asp Tyr Lys 
225                 230                 235                 240 
Lys Leu Val Trp Thr Asp Ser Met Thr Lys Gly Leu Thr Phe Asn Asn 
                245                 250                 255     
Asn Val Lys Val Thr Leu Asp Gly Glu Asp Phe Pro Val Leu Asn Tyr 
            260                 265                 270         
Lys Leu Val Thr Asp Asp Gln Gly Phe Arg Leu Ala Leu Asn Ala Thr 
        275                 280                 285             
Gly Leu Ala Ala Val Ala Ala Ala Ala Lys Asp Lys Asp Val Glu Ile 
    290                 295                 300                 
Lys Ile Thr Tyr Ser Ala Thr Val Asn Gly Ser Thr Thr Val Glu Ile 
305                 310                 315                 320 
Pro Glu Thr Asn Asp Val Lys Leu Asp Tyr Gly Asn Asn Pro Thr Glu 
                325                 330                 335     
Glu Ser Glu Pro Gln Glu Gly Thr Pro Ala Asn Gln Glu Ile Lys Val 
            340                 345                 350         
Ile Lys Asp Trp Ala Val Asp Gly Thr Ile Thr Asp Ala Asn Val Ala 
        355                 360                 365             
Val Lys Ala Ile Phe Thr Leu Gln Glu Lys Gln Thr Asp Gly Thr Trp 
    370                 375                 380                 
Val Asn Val Ala Ser His Glu Ala Thr Lys Pro Ser Arg Phe Glu His 
385                 390                 395                 400 
Thr Phe Thr Gly Leu Asp Asn Ala Lys Thr Tyr Arg Val Val Glu Arg 
                405                 410                 415     
Val Ser Gly Tyr Thr Pro Glu Tyr Val Ser Phe Lys Asn Gly Val Val 
            420                 425                 430         
Thr Ile Lys Asn Asn Lys Asn Ser Asn Asp Pro Thr Pro Ile Asn Pro 
        435                 440                 445             
Ser Glu Pro Lys Val Val Thr Tyr Gly Arg Lys Phe Val Lys Thr Asn 
    450                 455                 460                 
Gln Ala Asn Thr Glu Arg Leu Ala Gly Ala Thr Phe Leu Val Lys Lys 
465                 470                 475                 480 
Glu Gly Lys Tyr Leu Ala Arg Lys Ala Gly Ala Ala Thr Ala Glu Ala 
                485                 490                 495     
Lys Ala Ala Val Lys Thr Ala Lys Leu Ala Leu Asp Glu Ala Val Lys 
            500                 505                 510         
Ala Tyr Asn Asp Leu Thr Lys Glu Lys Gln Glu Gly Gln Glu Gly Lys 
        515                 520                 525             
Thr Ala Leu Ala Thr Val Asp Gln Lys Gln Lys Ala Tyr Asn Asp Ala 
    530                 535                 540                 
Phe Val Lys Ala Asn Tyr Ser Tyr Glu Trp Val Ala Asp Lys Lys Ala 
545                 550                 555                 560 
Asp Asn Val Val Lys Leu Ile Ser Asn Ala Gly Gly Gln Phe Glu Ile 
                565                 570                 575     
Thr Gly Leu Asp Lys Gly Thr Tyr Gly Leu Glu Glu Thr Gln Ala Pro 
            580                 585                 590         
Ala Gly Tyr Ala Thr Leu Ser Gly Asp Val Asn Phe Glu Val Thr Ala 
        595                 600                 605             
Thr Ser Tyr Ser Lys Gly Ala Thr Thr Asp Ile Ala Tyr Asp Lys Gly 
    610                 615                 620                 
Ser Val Lys Lys Asp Ala Gln Gln Val Gln Asn Lys Lys Val Thr Ile 
625                 630                 635                 640 
Pro Gln Thr Gly Gly Ile Gly Thr Ile Leu Phe Thr Ile Ile Gly Leu 
                645                 650                 655     
Ser Ile Met Leu Gly Ala Val Val Ile Met Lys Lys Arg Gln Ser Glu 
            660                 665                 670         
Glu Ala 
        

<210> 77
<211> 693
<212> PRT
<213> Streptococcus agalactiae CJB111

<220> 
<223> GBS59

<400> 77
Met Lys Arg Ile Asn Lys Tyr Phe Ala Met Phe Ser Ala Leu Leu Leu 
1               5                   10                  15      
Ile Leu Thr Ser Leu Leu Ser Val Ala Pro Val Phe Ala Ala Glu Met 
            20                  25                  30          
Gly Asn Ile Thr Lys Thr Val Thr Leu His Lys Ile Val Gln Thr Ser 
        35                  40                  45              
Asp Asn Leu Ala Lys Pro Asn Phe Pro Gly Ile Asn Gly Leu Asn Gly 
    50                  55                  60                  
Thr Lys Tyr Met Gly Gln Lys Leu Thr Asp Ile Ser Gly Tyr Phe Gly 
65                  70                  75                  80  
Gln Gly Ser Lys Glu Ile Ala Gly Ala Phe Phe Ala Val Met Asn Glu 
                85                  90                  95      
Ser Gln Thr Lys Tyr Ile Thr Glu Ser Gly Thr Glu Val Glu Ser Ile 
            100                 105                 110         
Asp Ala Ala Gly Val Leu Lys Gly Leu Thr Thr Glu Asn Gly Ile Thr 
        115                 120                 125             
Phe Asn Thr Ala Asn Leu Lys Gly Thr Tyr Gln Ile Val Glu Leu Leu 
    130                 135                 140                 
Asp Lys Ser Asn Tyr Lys Asn Gly Asp Lys Val Leu Ala Asp Ser Lys 
145                 150                 155                 160 
Ala Val Pro Val Lys Ile Thr Leu Pro Leu Tyr Asn Glu Glu Gly Ile 
                165                 170                 175     
Val Val Asp Ala Glu Val Tyr Pro Lys Asn Thr Glu Glu Ala Pro Gln 
            180                 185                 190         
Ile Asp Lys Asn Phe Ala Lys Ala Asn Lys Leu Leu Asn Asp Ser Asp 
        195                 200                 205             
Asn Ser Ala Ile Ala Gly Gly Ala Asp Tyr Asp Lys Tyr Gln Ala Glu 
    210                 215                 220                 
Lys Ala Lys Ala Thr Ala Glu Ile Gly Gln Glu Ile Pro Tyr Glu Val 
225                 230                 235                 240 
Lys Thr Lys Ile Gln Lys Gly Ser Lys Tyr Lys Asn Leu Ala Trp Val 
                245                 250                 255     
Asp Thr Met Ser Asn Gly Leu Thr Met Gly Asn Thr Val Asn Leu Glu 
            260                 265                 270         
Ala Ser Ser Gly Ser Phe Val Glu Gly Thr Asp Tyr Asn Val Glu Arg 
        275                 280                 285             
Asp Asp Arg Gly Phe Thr Leu Lys Phe Thr Asp Thr Gly Leu Thr Lys 
    290                 295                 300                 
Leu Gln Lys Glu Ala Glu Thr Gln Ala Val Glu Phe Thr Leu Thr Tyr 
305                 310                 315                 320 
Ser Ala Thr Val Asn Gly Ala Ala Ile Asp Asp Lys Pro Glu Ser Asn 
                325                 330                 335     
Asp Ile Lys Leu Gln Tyr Gly Asn Lys Pro Gly Lys Lys Val Lys Glu 
            340                 345                 350         
Ile Pro Val Thr Pro Ser Asn Gly Glu Ile Thr Val Ser Lys Thr Trp 
        355                 360                 365             
Asp Lys Gly Ser Asp Leu Glu Asn Ala Asn Val Val Tyr Thr Leu Lys 
    370                 375                 380                 
Asp Gly Gly Thr Ala Val Ala Ser Val Ser Leu Thr Lys Thr Thr Pro 
385                 390                 395                 400 
Asn Gly Glu Ile Asn Leu Gly Asn Gly Ile Lys Phe Thr Val Thr Gly 
                405                 410                 415     
Ala Phe Ala Gly Lys Phe Ser Gly Leu Thr Asp Ser Lys Thr Tyr Met 
            420                 425                 430         
Ile Ser Glu Arg Ile Ala Gly Tyr Gly Asn Thr Ile Thr Thr Gly Ala 
        435                 440                 445             
Gly Ser Ala Ala Ile Thr Asn Thr Pro Asp Ser Asp Asn Pro Thr Pro 
    450                 455                 460                 
Leu Asn Pro Thr Glu Pro Lys Val Val Thr His Gly Lys Lys Phe Val 
465                 470                 475                 480 
Lys Thr Ser Ser Thr Glu Thr Glu Arg Leu Gln Gly Ala Gln Phe Val 
                485                 490                 495     
Val Lys Asp Ser Ala Gly Lys Tyr Leu Ala Leu Lys Ser Ser Ala Thr 
            500                 505                 510         
Ile Ser Ala Gln Thr Thr Ala Tyr Thr Asn Ala Lys Thr Ala Leu Asp 
        515                 520                 525             
Ala Lys Ile Ala Ala Tyr Asn Lys Leu Ser Ala Asp Asp Gln Lys Gly 
    530                 535                 540                 
Thr Lys Gly Glu Thr Ala Lys Ala Glu Ile Lys Thr Ala Gln Asp Ala 
545                 550                 555                 560 
Tyr Asn Ala Ala Phe Ile Val Ala Arg Thr Ala Tyr Glu Trp Val Thr 
                565                 570                 575     
Asn Lys Glu Asp Ala Asn Val Val Lys Val Thr Ser Asn Ala Asp Gly 
            580                 585                 590         
Gln Phe Glu Val Ser Gly Leu Ala Thr Gly Asp Tyr Lys Leu Glu Glu 
        595                 600                 605             
Thr Gln Ala Pro Ala Gly Tyr Ala Lys Leu Ala Gly Asp Val Asp Phe 
    610                 615                 620                 
Lys Val Gly Asn Ser Ser Lys Ala Asp Asp Ser Gly Asn Ile Asp Tyr 
625                 630                 635                 640 
Thr Ala Ser Ser Asn Lys Lys Asp Ala Gln Arg Ile Glu Asn Lys Lys 
                645                 650                 655     
Val Thr Ile Pro Gln Thr Gly Gly Ile Gly Thr Ile Leu Phe Thr Ile 
            660                 665                 670         
Ile Gly Leu Ser Ile Met Leu Gly Ala Val Ile Ile Met Lys Arg Arg 
        675                 680                 685             
Gln Ser Glu Glu Ala 
    690             

<210> 78
<211> 704
<212> PRT
<213> Streptococcus agalactiae CJB110

<220> 
<223> GBS59

<400> 78
Met Lys Lys Ile Asn Lys Tyr Phe Ala Val Phe Ser Ala Leu Leu Leu 
1               5                   10                  15      
Thr Val Thr Ser Leu Leu Ser Val Ala Pro Ala Phe Ala Asp Glu Ala 
            20                  25                  30          
Thr Thr Asn Thr Val Thr Leu His Lys Ile Leu Gln Thr Glu Ser Asn 
        35                  40                  45              
Leu Asn Lys Ser Asn Phe Pro Gly Thr Thr Gly Leu Asn Gly Asp Asp 
    50                  55                  60                  
Tyr Lys Gly Glu Ser Ile Ser Asp Leu Ala Glu Tyr Phe Gly Ser Gly 
65                  70                  75                  80  
Ser Lys Glu Ile Asp Gly Ala Phe Phe Ala Leu Ala Leu Glu Glu Glu 
                85                  90                  95      
Lys Asp Gly Val Val Gln Tyr Val Lys Ala Lys Ala Asn Asp Lys Leu 
            100                 105                 110         
Thr Pro Asp Leu Ile Thr Lys Gly Thr Pro Ala Thr Thr Thr Lys Val 
        115                 120                 125             
Glu Glu Ala Val Gly Gly Leu Thr Thr Gly Thr Gly Ile Val Phe Asn 
    130                 135                 140                 
Thr Ala Gly Leu Lys Gly Asn Phe Lys Ile Ile Glu Leu Lys Asp Lys 
145                 150                 155                 160 
Ser Thr Tyr Asn Asn Asn Gly Ser Leu Leu Ala Ala Ser Lys Ala Val 
                165                 170                 175     
Pro Val Lys Ile Thr Leu Pro Leu Val Ser Lys Asp Gly Val Val Lys 
            180                 185                 190         
Asp Ala His Val Tyr Pro Lys Asn Thr Glu Thr Lys Pro Glu Val Asp 
        195                 200                 205             
Lys Asn Phe Ala Lys Thr Asn Asp Leu Thr Ala Leu Lys Asp Ala Thr 
    210                 215                 220                 
Leu Leu Lys Ala Gly Ala Asp Tyr Lys Asn Tyr Ser Ala Thr Lys Ala 
225                 230                 235                 240 
Thr Val Thr Ala Glu Ile Gly Lys Val Ile Pro Tyr Glu Val Lys Thr 
                245                 250                 255     
Lys Val Leu Lys Gly Ser Lys Tyr Glu Lys Leu Val Trp Thr Asp Thr 
            260                 265                 270         
Met Ser Asn Gly Leu Thr Met Gly Asp Asp Val Asn Leu Ala Val Ser 
        275                 280                 285             
Gly Thr Thr Thr Thr Phe Ile Lys Asp Ile Asp Tyr Thr Leu Ser Ile 
    290                 295                 300                 
Asp Asp Arg Gly Phe Thr Leu Lys Phe Lys Ala Thr Gly Leu Asp Lys 
305                 310                 315                 320 
Leu Glu Glu Ala Ala Lys Ala Ser Asp Val Glu Phe Thr Leu Thr Tyr 
                325                 330                 335     
Lys Ala Thr Val Asn Gly Gln Ala Ile Ile Asp Asn Pro Glu Val Asn 
            340                 345                 350         
Asp Ile Lys Leu Asp Tyr Gly Asn Lys Pro Gly Thr Asp Leu Ser Glu 
        355                 360                 365             
Gln Pro Val Thr Pro Glu Asp Gly Glu Val Lys Val Thr Lys Thr Trp 
    370                 375                 380                 
Ala Ala Gly Ala Asn Lys Ala Asp Ala Lys Val Val Tyr Thr Leu Lys 
385                 390                 395                 400 
Asn Ala Thr Lys Gln Val Val Ala Ser Val Ala Leu Thr Ala Ala Asp 
                405                 410                 415     
Thr Lys Gly Thr Ile Asn Leu Gly Lys Gly Met Thr Phe Glu Ile Thr 
            420                 425                 430         
Gly Ala Phe Ser Gly Thr Phe Lys Gly Leu Gln Asn Lys Ala Tyr Thr 
        435                 440                 445             
Val Ser Glu Arg Val Ala Gly Tyr Thr Asn Ala Ile Asn Val Thr Gly 
    450                 455                 460                 
Asn Ala Val Ala Ile Thr Asn Thr Pro Asp Ser Asp Asn Pro Thr Pro 
465                 470                 475                 480 
Leu Asn Pro Thr Gln Pro Lys Val Glu Thr His Gly Lys Lys Phe Val 
                485                 490                 495     
Lys Val Gly Asp Ala Asp Ala Arg Leu Ala Gly Ala Gln Phe Val Val 
            500                 505                 510         
Lys Asn Ser Ala Gly Lys Phe Leu Ala Leu Lys Glu Asp Ala Ala Val 
        515                 520                 525             
Ser Gly Ala Gln Thr Glu Leu Ala Thr Ala Lys Thr Asp Leu Asp Asn 
    530                 535                 540                 
Ala Ile Lys Ala Tyr Asn Gly Leu Thr Lys Ala Gln Gln Glu Gly Ala 
545                 550                 555                 560 
Asp Gly Thr Ser Ala Lys Glu Leu Ile Asn Thr Lys Gln Ser Ala Tyr 
                565                 570                 575     
Asp Ala Ala Phe Ile Lys Ala Arg Thr Ala Tyr Thr Trp Val Asp Glu 
            580                 585                 590         
Lys Thr Lys Ala Ile Thr Phe Thr Ser Asn Asn Gln Gly Gln Phe Glu 
        595                 600                 605             
Val Thr Gly Leu Glu Val Gly Ser Tyr Lys Leu Glu Glu Thr Leu Ala 
    610                 615                 620                 
Pro Ala Gly Tyr Ala Lys Leu Ser Gly Asp Ile Glu Phe Thr Val Gly 
625                 630                 635                 640 
His Asp Ser Tyr Thr Ser Gly Asp Ile Lys Tyr Lys Thr Asp Asp Ala 
                645                 650                 655     
Ser Asn Asn Ala Gln Lys Val Phe Asn Lys Lys Val Thr Ile Pro Gln 
            660                 665                 670         
Thr Gly Gly Ile Gly Thr Ile Leu Phe Thr Ile Ile Gly Leu Ser Ile 
        675                 680                 685             
Met Leu Gly Ala Val Val Ile Met Lys Arg Arg Gln Ser Glu Glu Ala 
    690                 695                 700                 



<210> 79
<211> 682
<212> PRT
<213> Streptococcus agalactiae DK21

<220> 
<223> GBS59

<400> 79
Met Lys Lys Ile Asn Lys Phe Phe Val Ala Phe Ser Ala Leu Leu Leu 
1               5                   10                  15      
Ile Leu Thr Ser Leu Leu Ser Val Ala Pro Ala Phe Ala Glu Glu Glu 
            20                  25                  30          
Arg Thr Thr Glu Thr Val Thr Leu His Lys Ile Leu Gln Thr Glu Thr 
        35                  40                  45              
Asn Leu Lys Asn Ser Ala Phe Pro Gly Thr Lys Gly Leu Asp Gly Thr 
    50                  55                  60                  
Glu Tyr Asp Gly Lys Ala Ile Asp Lys Leu Asp Ser Tyr Phe Gly Asn 
65                  70                  75                  80  
Asp Ser Lys Asp Ile Gly Gly Ala Tyr Phe Ile Leu Ala Asn Ser Lys 
                85                  90                  95      
Gly Glu Tyr Ile Lys Ala Asn Asp Lys Asn Lys Leu Lys Pro Glu Phe 
            100                 105                 110         
Ser Gly Asn Thr Pro Lys Thr Thr Leu Asn Ile Ser Glu Ala Val Gly 
        115                 120                 125             
Gly Leu Thr Glu Glu Asn Ala Gly Ile Lys Phe Glu Thr Thr Gly Leu 
    130                 135                 140                 
Arg Gly Asp Phe Gln Ile Ile Glu Leu Lys Asp Lys Ser Thr Tyr Asn 
145                 150                 155                 160 
Asn Gly Gly Ala Ile Leu Ala Asp Ser Lys Ala Val Pro Val Lys Ile 
                165                 170                 175     
Thr Leu Pro Leu Ile Asn Lys Asp Gly Val Val Lys Asp Ala His Val 
            180                 185                 190         
Tyr Pro Lys Asn Thr Glu Thr Lys Pro Gln Ile Asp Lys Asn Phe Ala 
        195                 200                 205             
Asp Lys Asn Leu Asp Tyr Ile Asn Asn Gln Lys Asp Lys Gly Thr Ile 
    210                 215                 220                 
Ser Ala Thr Val Gly Asp Val Lys Lys Tyr Thr Val Gly Thr Lys Ile 
225                 230                 235                 240 
Leu Lys Gly Ser Asp Tyr Lys Lys Leu Val Trp Thr Asp Ser Met Thr 
                245                 250                 255     
Lys Gly Leu Thr Phe Asn Asn Asp Val Thr Val Thr Leu Asp Gly Ala 
            260                 265                 270         
Asn Phe Glu Gln Ser Asn Tyr Thr Leu Val Ala Asp Asp Gln Gly Phe 
        275                 280                 285             
Arg Leu Val Leu Asn Ala Thr Gly Leu Ser Lys Val Ala Glu Ala Ala 
    290                 295                 300                 
Lys Thr Lys Asp Val Glu Ile Lys Ile Asn Tyr Ser Ala Thr Val Asn 
305                 310                 315                 320 
Gly Ser Thr Val Val Glu Lys Ser Glu Asn Asn Asp Val Lys Leu Asp 
                325                 330                 335     
Tyr Gly Asn Asn Pro Thr Thr Glu Asn Glu Pro Gln Thr Gly Asn Pro 
            340                 345                 350         
Val Asn Lys Glu Ile Thr Val Arg Lys Thr Trp Ala Val Asp Gly Asn 
        355                 360                 365             
Glu Val Asn Lys Gly Asp Glu Lys Val Asp Ala Val Phe Thr Leu Gln 
    370                 375                 380                 
Val Lys Asp Ser Asp Lys Trp Val Asn Val Asp Ser Ala Thr Ala Thr 
385                 390                 395                 400 
Ala Ala Thr Asp Phe Lys Tyr Thr Phe Lys Asn Leu Asp Asn Ala Lys 
                405                 410                 415     
Thr Tyr Arg Val Val Glu Arg Val Ser Gly Tyr Ala Pro Ala Tyr Val 
            420                 425                 430         
Ser Phe Val Gly Gly Val Val Thr Ile Lys Asn Asn Lys Asn Ser Asn 
        435                 440                 445             
Asp Pro Thr Pro Ile Asn Pro Ser Glu Pro Lys Val Val Thr Tyr Gly 
    450                 455                 460                 
Arg Lys Phe Val Lys Thr Asn Gln Asp Gly Ser Glu Arg Leu Ala Gly 
465                 470                 475                 480 
Ala Thr Phe Leu Val Lys Asn Ser Gln Ser Gln Tyr Leu Ala Arg Lys 
                485                 490                 495     
Ser Gly Val Ala Thr Asn Glu Ala His Lys Ala Val Thr Asp Ala Lys 
            500                 505                 510         
Val Gln Leu Asp Glu Ala Val Lys Ala Tyr Asn Lys Leu Thr Lys Glu 
        515                 520                 525             
Gln Gln Glu Ser Gln Asp Gly Lys Ala Ala Leu Asn Leu Ile Asp Glu 
    530                 535                 540                 
Lys Gln Thr Ala Tyr Asn Glu Ala Phe Ala Lys Ala Asn Tyr Ser Tyr 
545                 550                 555                 560 
Glu Trp Val Val Asp Lys Asn Ala Ala Asn Val Val Lys Leu Ile Ser 
                565                 570                 575     
Asn Thr Ala Gly Lys Phe Glu Ile Thr Gly Leu Asn Ala Gly Glu Tyr 
            580                 585                 590         
Ser Leu Glu Glu Thr Gln Ala Pro Thr Gly Tyr Ala Lys Leu Ser Ser 
        595                 600                 605             
Asp Val Ser Phe Lys Val Asn Asp Thr Ser Tyr Ser Glu Gly Ala Ser 
    610                 615                 620                 
Asn Asp Ile Ala Tyr Asp Lys Asp Ser Gly Lys Thr Asp Ala Gln Lys 
625                 630                 635                 640 
Val Val Asn Lys Lys Val Thr Ile Pro Gln Thr Gly Gly Ile Gly Thr 
                645                 650                 655     
Ile Leu Phe Thr Ile Ile Gly Leu Ser Ile Met Leu Gly Ala Val Val 
            660                 665                 670         
Ile Met Lys Arg Arg Gln Ser Glu Glu Ala 
        675                 680         

<210> 80
<211> 502
<212> PRT
<213> Streptococcus agalactiae COH1

<220> 
<223> spb1

<400> 80
Met Lys Lys Lys Met Ile Gln Ser Leu Leu Val Ala Ser Leu Ala Phe 
1               5                   10                  15      
Gly Met Ala Val Ser Pro Val Thr Pro Ile Ala Phe Ala Ala Glu Thr 
            20                  25                  30          
Gly Thr Ile Thr Val Gln Asp Thr Gln Lys Gly Ala Thr Tyr Lys Ala 
        35                  40                  45              
Tyr Lys Val Phe Asp Ala Glu Ile Asp Asn Ala Asn Val Ser Asp Ser 
    50                  55                  60                  
Asn Lys Asp Gly Ala Ser Tyr Leu Ile Pro Gln Gly Lys Glu Ala Glu 
65                  70                  75                  80  
Tyr Lys Ala Ser Thr Asp Phe Asn Ser Leu Phe Thr Thr Thr Thr Asn 
                85                  90                  95      
Gly Gly Arg Thr Tyr Val Thr Lys Lys Asp Thr Ala Ser Ala Asn Glu 
            100                 105                 110         
Ile Ala Thr Trp Ala Lys Ser Ile Ser Ala Asn Thr Thr Pro Val Ser 
        115                 120                 125             
Thr Val Thr Glu Ser Asn Asn Asp Gly Thr Glu Val Ile Asn Val Ser 
    130                 135                 140                 
Gln Tyr Gly Tyr Tyr Tyr Val Ser Ser Thr Val Asn Asn Gly Ala Val 
145                 150                 155                 160 
Ile Met Val Thr Ser Val Thr Pro Asn Ala Thr Ile His Glu Lys Asn 
                165                 170                 175     
Thr Asp Ala Thr Trp Gly Asp Gly Gly Gly Lys Thr Val Asp Gln Lys 
            180                 185                 190         
Thr Tyr Ser Val Gly Asp Thr Val Lys Tyr Thr Ile Thr Tyr Lys Asn 
        195                 200                 205             
Ala Val Asn Tyr His Gly Thr Glu Lys Val Tyr Gln Tyr Val Ile Lys 
    210                 215                 220                 
Asp Thr Met Pro Ser Ala Ser Val Val Asp Leu Asn Glu Gly Ser Tyr 
225                 230                 235                 240 
Glu Val Thr Ile Thr Asp Gly Ser Gly Asn Ile Thr Thr Leu Thr Gln 
                245                 250                 255     
Gly Ser Glu Lys Ala Thr Gly Lys Tyr Asn Leu Leu Glu Glu Asn Asn 
            260                 265                 270         
Asn Phe Thr Ile Thr Ile Pro Trp Ala Ala Thr Asn Thr Pro Thr Gly 
        275                 280                 285             
Asn Thr Gln Asn Gly Ala Asn Asp Asp Phe Phe Tyr Lys Gly Ile Asn 
    290                 295                 300                 
Thr Ile Thr Val Thr Tyr Thr Gly Val Leu Lys Ser Gly Ala Lys Pro 
305                 310                 315                 320 
Gly Ser Ala Asp Leu Pro Glu Asn Thr Asn Ile Ala Thr Ile Asn Pro 
                325                 330                 335     
Asn Thr Ser Asn Asp Asp Pro Gly Gln Lys Val Thr Val Arg Asp Gly 
            340                 345                 350         
Gln Ile Thr Ile Lys Lys Ile Asp Gly Ser Thr Lys Ala Ser Leu Gln 
        355                 360                 365             
Gly Ala Ile Phe Val Leu Lys Asn Ala Thr Gly Gln Phe Leu Asn Phe 
    370                 375                 380                 
Asn Asp Thr Asn Asn Val Glu Trp Gly Thr Glu Ala Asn Ala Thr Glu 
385                 390                 395                 400 
Tyr Thr Thr Gly Ala Asp Gly Ile Ile Thr Ile Thr Gly Leu Lys Glu 
                405                 410                 415     
Gly Thr Tyr Tyr Leu Val Glu Lys Lys Ala Pro Leu Gly Tyr Asn Leu 
            420                 425                 430         
Leu Asp Asn Ser Gln Lys Val Ile Leu Gly Asp Gly Ala Thr Asp Thr 
        435                 440                 445             
Thr Asn Ser Asp Asn Leu Leu Val Asn Pro Thr Val Glu Asn Asn Lys 
    450                 455                 460                 
Gly Thr Glu Leu Pro Ser Thr Gly Gly Ile Gly Thr Thr Ile Phe Tyr 
465                 470                 475                 480 
Ile Ile Gly Ala Ile Leu Val Ile Gly Ala Gly Ile Val Leu Val Ala 
                485                 490                 495     
Arg Arg Arg Leu Arg Ser 
            500         

<210> 81
<211> 438
<212> PRT
<213> Streptococcus agalactiae COH1

<220> 
<223> Spb1 Leader/Signal sequence removed

<400> 81
Ala Glu Thr Gly Thr Ile Thr Val Gln Asp Thr Gln Lys Gly Ala Thr 
1               5                   10                  15      
Tyr Lys Ala Tyr Lys Val Phe Asp Ala Glu Ile Asp Asn Ala Asn Val 
            20                  25                  30          
Ser Asp Ser Asn Lys Asp Gly Ala Ser Tyr Leu Ile Pro Gln Gly Lys 
        35                  40                  45              
Glu Ala Glu Tyr Lys Ala Ser Thr Asp Phe Asn Ser Leu Phe Thr Thr 
    50                  55                  60                  
Thr Thr Asn Gly Gly Arg Thr Tyr Val Thr Lys Lys Asp Thr Ala Ser 
65                  70                  75                  80  
Ala Asn Glu Ile Ala Thr Trp Ala Lys Ser Ile Ser Ala Asn Thr Thr 
                85                  90                  95      
Pro Val Ser Thr Val Thr Glu Ser Asn Asn Asp Gly Thr Glu Val Ile 
            100                 105                 110         
Asn Val Ser Gln Tyr Gly Tyr Tyr Tyr Val Ser Ser Thr Val Asn Asn 
        115                 120                 125             
Gly Ala Val Ile Met Val Thr Ser Val Thr Pro Asn Ala Thr Ile His 
    130                 135                 140                 
Glu Lys Asn Thr Asp Ala Thr Trp Gly Asp Gly Gly Gly Lys Thr Val 
145                 150                 155                 160 
Asp Gln Lys Thr Tyr Ser Val Gly Asp Thr Val Lys Tyr Thr Ile Thr 
                165                 170                 175     
Tyr Lys Asn Ala Val Asn Tyr His Gly Thr Glu Lys Val Tyr Gln Tyr 
            180                 185                 190         
Val Ile Lys Asp Thr Met Pro Ser Ala Ser Val Val Asp Leu Asn Glu 
        195                 200                 205             
Gly Ser Tyr Glu Val Thr Ile Thr Asp Gly Ser Gly Asn Ile Thr Thr 
    210                 215                 220                 
Leu Thr Gln Gly Ser Glu Lys Ala Thr Gly Lys Tyr Asn Leu Leu Glu 
225                 230                 235                 240 
Glu Asn Asn Asn Phe Thr Ile Thr Ile Pro Trp Ala Ala Thr Asn Thr 
                245                 250                 255     
Pro Thr Gly Asn Thr Gln Asn Gly Ala Asn Asp Asp Phe Phe Tyr Lys 
            260                 265                 270         
Gly Ile Asn Thr Ile Thr Val Thr Tyr Thr Gly Val Leu Lys Ser Gly 
        275                 280                 285             
Ala Lys Pro Gly Ser Ala Asp Leu Pro Glu Asn Thr Asn Ile Ala Thr 
    290                 295                 300                 
Ile Asn Pro Asn Thr Ser Asn Asp Asp Pro Gly Gln Lys Val Thr Val 
305                 310                 315                 320 
Arg Asp Gly Gln Ile Thr Ile Lys Lys Ile Asp Gly Ser Thr Lys Ala 
                325                 330                 335     
Ser Leu Gln Gly Ala Ile Phe Val Leu Lys Asn Ala Thr Gly Gln Phe 
            340                 345                 350         
Leu Asn Phe Asn Asp Thr Asn Asn Val Glu Trp Gly Thr Glu Ala Asn 
        355                 360                 365             
Ala Thr Glu Tyr Thr Thr Gly Ala Asp Gly Ile Ile Thr Ile Thr Gly 
    370                 375                 380                 
Leu Lys Glu Gly Thr Tyr Tyr Leu Val Glu Lys Lys Ala Pro Leu Gly 
385                 390                 395                 400 
Tyr Asn Leu Leu Asp Asn Ser Gln Lys Val Ile Leu Gly Asp Gly Ala 
                405                 410                 415     
Thr Asp Thr Thr Asn Ser Asp Asn Leu Leu Val Asn Pro Thr Val Glu 
            420                 425                 430         
Asn Asn Lys Gly Thr Glu 
        435             

<210> 82
<211> 665
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> RrgB

<400> 82
Met Lys Ser Ile Asn Lys Phe Leu Thr Met Leu Ala Ala Leu Leu Leu 
1               5                   10                  15      
Thr Ala Ser Ser Leu Phe Ser Ala Ala Thr Val Phe Ala Ala Gly Thr 
            20                  25                  30          
Thr Thr Thr Ser Val Thr Val His Lys Leu Leu Ala Thr Asp Gly Asp 
        35                  40                  45              
Met Asp Lys Ile Ala Asn Glu Leu Glu Thr Gly Asn Tyr Ala Gly Asn 
    50                  55                  60                  
Lys Val Gly Val Leu Pro Ala Asn Ala Lys Glu Ile Ala Gly Val Met 
65                  70                  75                  80  
Phe Val Trp Thr Asn Thr Asn Asn Glu Ile Ile Asp Glu Asn Gly Gln 
                85                  90                  95      
Thr Leu Gly Val Asn Ile Asp Pro Gln Thr Phe Lys Leu Ser Gly Ala 
            100                 105                 110         
Met Pro Ala Thr Ala Met Lys Lys Leu Thr Glu Ala Glu Gly Ala Lys 
        115                 120                 125             
Phe Asn Thr Ala Asn Leu Pro Ala Ala Lys Tyr Lys Ile Tyr Glu Ile 
    130                 135                 140                 
His Ser Leu Ser Thr Tyr Val Gly Glu Asp Gly Ala Thr Leu Thr Gly 
145                 150                 155                 160 
Ser Lys Ala Val Pro Ile Glu Ile Glu Leu Pro Leu Asn Asp Val Val 
                165                 170                 175     
Asp Ala His Val Tyr Pro Lys Asn Thr Glu Ala Lys Pro Lys Ile Asp 
            180                 185                 190         
Lys Asp Phe Lys Gly Lys Ala Asn Pro Asp Thr Pro Arg Val Asp Lys 
        195                 200                 205             
Asp Thr Pro Val Asn His Gln Val Gly Asp Val Val Glu Tyr Glu Ile 
    210                 215                 220                 
Val Thr Lys Ile Pro Ala Leu Ala Asn Tyr Ala Thr Ala Asn Trp Ser 
225                 230                 235                 240 
Asp Arg Met Thr Glu Gly Leu Ala Phe Asn Lys Gly Thr Val Lys Val 
                245                 250                 255     
Thr Val Asp Asp Val Ala Leu Glu Ala Gly Asp Tyr Ala Leu Thr Glu 
            260                 265                 270         
Val Ala Thr Gly Phe Asp Leu Lys Leu Thr Asp Ala Gly Leu Ala Lys 
        275                 280                 285             
Val Asn Asp Gln Asn Ala Glu Lys Thr Val Lys Ile Thr Tyr Ser Ala 
    290                 295                 300                 
Thr Leu Asn Asp Lys Ala Ile Val Glu Val Pro Glu Ser Asn Asp Val 
305                 310                 315                 320 
Thr Phe Asn Tyr Gly Asn Asn Pro Asp His Gly Asn Thr Pro Lys Pro 
                325                 330                 335     
Asn Lys Pro Asn Glu Asn Gly Asp Leu Thr Leu Thr Lys Thr Trp Val 
            340                 345                 350         
Asp Ala Thr Gly Ala Pro Ile Pro Ala Gly Ala Glu Ala Thr Phe Asp 
        355                 360                 365             
Leu Val Asn Ala Gln Thr Gly Lys Val Val Gln Thr Val Thr Leu Thr 
    370                 375                 380                 
Thr Asp Lys Asn Thr Val Thr Val Asn Gly Leu Asp Lys Asn Thr Glu 
385                 390                 395                 400 
Tyr Lys Phe Val Glu Arg Ser Ile Lys Gly Tyr Ser Ala Asp Tyr Gln 
                405                 410                 415     
Glu Ile Thr Thr Ala Gly Glu Ile Ala Val Lys Asn Trp Lys Asp Glu 
            420                 425                 430         
Asn Pro Lys Pro Leu Asp Pro Thr Glu Pro Lys Val Val Thr Tyr Gly 
        435                 440                 445             
Lys Lys Phe Val Lys Val Asn Asp Lys Asp Asn Arg Leu Ala Gly Ala 
    450                 455                 460                 
Glu Phe Val Ile Ala Asn Ala Asp Asn Ala Gly Gln Tyr Leu Ala Arg 
465                 470                 475                 480 
Lys Ala Asp Lys Val Ser Gln Glu Glu Lys Gln Leu Val Val Thr Thr 
                485                 490                 495     
Lys Asp Ala Leu Asp Arg Ala Val Ala Ala Tyr Asn Ala Leu Thr Ala 
            500                 505                 510         
Gln Gln Gln Thr Gln Gln Glu Lys Glu Lys Val Asp Lys Ala Gln Ala 
        515                 520                 525             
Ala Tyr Asn Ala Ala Val Ile Ala Ala Asn Asn Ala Phe Glu Trp Val 
    530                 535                 540                 
Ala Asp Lys Asp Asn Glu Asn Val Val Lys Leu Val Ser Asp Ala Gln 
545                 550                 555                 560 
Gly Arg Phe Glu Ile Thr Gly Leu Leu Ala Gly Thr Tyr Tyr Leu Glu 
                565                 570                 575     
Glu Thr Lys Gln Pro Ala Gly Tyr Ala Leu Leu Thr Ser Arg Gln Lys 
            580                 585                 590         
Phe Glu Val Thr Ala Thr Ser Tyr Ser Ala Thr Gly Gln Gly Ile Glu 
        595                 600                 605             
Tyr Thr Ala Gly Ser Gly Lys Asp Asp Ala Thr Lys Val Val Asn Lys 
    610                 615                 620                 
Lys Ile Thr Ile Pro Gln Thr Gly Gly Ile Gly Thr Ile Ile Phe Ala 
625                 630                 635                 640 
Val Ala Gly Ala Ala Ile Met Gly Ile Ala Val Tyr Ala Tyr Val Lys 
                645                 650                 655     
Asn Asn Lys Asp Glu Asp Gln Leu Ala 
            660                 665 

<210> 83
<211> 644
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> RrgB

<400> 83
Met Lys Ser Ile Asn Lys Phe Leu Thr Met Leu Ala Ala Leu Leu Leu 
1               5                   10                  15      
Thr Ala Ser Ser Leu Phe Ser Ala Ala Thr Val Phe Ala Ala Asp Asn 
            20                  25                  30          
Val Ser Thr Ala Pro Asp Ala Val Thr Lys Thr Leu Thr Ile His Lys 
        35                  40                  45              
Leu Leu Leu Ser Glu Asp Asp Leu Lys Thr Trp Asp Thr Asn Gly Pro 
    50                  55                  60                  
Lys Gly Tyr Asp Gly Thr Gln Ser Ser Leu Lys Asp Leu Thr Gly Val 
65                  70                  75                  80  
Val Ala Glu Glu Ile Pro Asn Val Tyr Phe Glu Leu Gln Lys Tyr Asn 
                85                  90                  95      
Leu Thr Asp Gly Lys Glu Lys Glu Asn Leu Lys Asp Asp Ser Lys Trp 
            100                 105                 110         
Thr Thr Val His Gly Gly Leu Thr Thr Lys Asp Gly Leu Lys Ile Glu 
        115                 120                 125             
Thr Ser Thr Leu Lys Gly Val Tyr Arg Ile Arg Glu Asp Arg Thr Lys 
    130                 135                 140                 
Thr Thr Tyr Val Gly Pro Asn Gly Gln Val Leu Thr Gly Ser Lys Ala 
145                 150                 155                 160 
Val Pro Ala Leu Val Thr Leu Pro Leu Val Asn Asn Asn Gly Thr Val 
                165                 170                 175     
Ile Asp Ala His Val Phe Pro Lys Asn Ser Tyr Asn Lys Pro Val Val 
            180                 185                 190         
Asp Lys Arg Ile Ala Asp Thr Leu Asn Tyr Asn Asp Gln Asn Gly Leu 
        195                 200                 205             
Ser Ile Gly Thr Lys Ile Pro Tyr Val Val Asn Thr Thr Ile Pro Ser 
    210                 215                 220                 
Asn Ala Thr Phe Ala Thr Ser Phe Trp Ser Asp Glu Met Thr Glu Gly 
225                 230                 235                 240 
Leu Thr Tyr Asn Glu Asp Val Thr Ile Thr Leu Asn Asn Val Ala Met 
                245                 250                 255     
Asp Gln Ala Asp Tyr Glu Val Thr Lys Gly Asn Asn Gly Phe Asn Leu 
            260                 265                 270         
Lys Leu Thr Glu Ala Gly Leu Ala Lys Ile Asn Gly Lys Asp Ala Asp 
        275                 280                 285             
Gln Lys Ile Gln Ile Thr Tyr Ser Ala Thr Leu Asn Ser Leu Ala Val 
    290                 295                 300                 
Ala Asp Ile Pro Glu Ser Asn Asp Ile Thr Tyr His Tyr Gly Asn His 
305                 310                 315                 320 
Gln Asp His Gly Asn Thr Pro Lys Pro Thr Lys Pro Asn Asn Gly Gln 
                325                 330                 335     
Ile Thr Val Thr Lys Thr Trp Asp Ser Gln Pro Ala Pro Glu Gly Val 
            340                 345                 350         
Lys Ala Thr Val Gln Leu Val Asn Ala Lys Thr Gly Glu Lys Val Gly 
        355                 360                 365             
Ala Pro Val Glu Leu Ser Glu Asn Asn Trp Thr Tyr Thr Trp Ser Gly 
    370                 375                 380                 
Leu Asp Asn Ser Ile Glu Tyr Lys Val Glu Glu Glu Tyr Asn Gly Tyr 
385                 390                 395                 400 
Ser Ala Glu Tyr Thr Val Glu Ser Lys Gly Lys Leu Gly Val Lys Asn 
                405                 410                 415     
Trp Lys Asp Asn Asn Pro Ala Pro Ile Asn Pro Glu Glu Pro Arg Val 
            420                 425                 430         
Lys Thr Tyr Gly Lys Lys Phe Val Lys Val Asp Gln Lys Asp Thr Arg 
        435                 440                 445             
Leu Glu Asn Ala Gln Phe Val Val Lys Lys Ala Asp Ser Asn Lys Tyr 
    450                 455                 460                 
Ile Ala Phe Lys Ser Thr Ala Gln Gln Ala Ala Asp Glu Lys Ala Ala 
465                 470                 475                 480 
Ala Thr Ala Lys Gln Lys Leu Asp Ala Ala Val Ala Ala Tyr Thr Asn 
                485                 490                 495     
Ala Ala Asp Lys Gln Ala Ala Gln Ala Leu Val Asp Gln Ala Gln Gln 
            500                 505                 510         
Glu Tyr Asn Val Ala Tyr Lys Glu Ala Lys Phe Gly Tyr Val Glu Val 
        515                 520                 525             
Ala Gly Lys Asp Glu Ala Met Val Leu Thr Ser Asn Thr Asp Gly Gln 
    530                 535                 540                 
Phe Gln Ile Ser Gly Leu Ala Ala Gly Thr Tyr Lys Leu Glu Glu Ile 
545                 550                 555                 560 
Lys Ala Pro Glu Gly Phe Ala Lys Ile Asp Asp Val Glu Phe Val Val 
                565                 570                 575     
Gly Ala Gly Ser Trp Asn Gln Gly Glu Phe Asn Tyr Leu Lys Asp Val 
            580                 585                 590         
Gln Lys Asn Asp Ala Thr Lys Val Val Asn Lys Lys Ile Thr Ile Pro 
        595                 600                 605             
Gln Thr Gly Gly Ile Gly Thr Ile Ile Phe Ala Val Ala Gly Ala Ala 
    610                 615                 620                 
Ile Met Gly Ile Ala Val Tyr Ala Tyr Val Lys Asn Asn Lys Asp Glu 
625                 630                 635                 640 
Asp Gln Leu Ala 
                

<210> 84
<211> 654
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> RrgB

<400> 84
Met Lys Ser Ile Asn Lys Phe Leu Thr Ile Leu Ala Ala Leu Leu Leu 
1               5                   10                  15      
Thr Val Ser Ser Leu Phe Ser Ala Ala Thr Val Phe Ala Ala Glu Gln 
            20                  25                  30          
Lys Thr Lys Thr Leu Thr Val His Lys Leu Leu Met Thr Asp Gln Glu 
        35                  40                  45              
Leu Asp Ala Trp Asn Ser Asp Ala Ile Thr Thr Ala Gly Tyr Asp Gly 
    50                  55                  60                  
Ser Gln Asn Phe Glu Gln Phe Lys Gln Leu Gln Gly Val Pro Gln Gly 
65                  70                  75                  80  
Val Thr Glu Ile Ser Gly Val Ala Phe Glu Leu Gln Ser Tyr Thr Gly 
                85                  90                  95      
Pro Gln Gly Lys Glu Gln Glu Asn Leu Thr Asn Asp Ala Val Trp Thr 
            100                 105                 110         
Ala Val Asn Lys Gly Val Thr Thr Glu Thr Gly Val Lys Phe Asp Thr 
        115                 120                 125             
Glu Val Leu Gln Gly Thr Tyr Arg Leu Val Glu Val Arg Lys Glu Ser 
    130                 135                 140                 
Thr Tyr Val Gly Pro Asn Gly Lys Val Leu Thr Gly Met Lys Ala Val 
145                 150                 155                 160 
Pro Ala Leu Ile Thr Leu Pro Leu Val Asn Gln Asn Gly Val Val Glu 
                165                 170                 175     
Asn Ala His Val Tyr Pro Lys Asn Ser Glu Asp Lys Pro Thr Ala Thr 
            180                 185                 190         
Lys Thr Phe Asp Thr Ala Ala Gly Phe Val Asp Pro Gly Glu Lys Gly 
        195                 200                 205             
Leu Ala Ile Gly Thr Lys Val Pro Tyr Ile Val Thr Thr Thr Ile Pro 
    210                 215                 220                 
Lys Asn Ser Thr Leu Ala Thr Ala Phe Trp Ser Asp Glu Met Thr Glu 
225                 230                 235                 240 
Gly Leu Asp Tyr Asn Gly Asp Val Val Val Asn Tyr Asn Gly Gln Pro 
                245                 250                 255     
Leu Asp Asn Ser His Tyr Thr Leu Glu Ala Gly His Asn Gly Phe Ile 
            260                 265                 270         
Leu Lys Leu Asn Glu Lys Gly Leu Glu Ala Ile Asn Gly Lys Asp Ala 
        275                 280                 285             
Glu Ala Thr Ile Thr Leu Lys Tyr Thr Ala Thr Leu Asn Ala Leu Ala 
    290                 295                 300                 
Val Ala Asp Val Pro Glu Ala Asn Asp Val Thr Phe His Tyr Gly Asn 
305                 310                 315                 320 
Asn Pro Gly His Gly Asn Thr Pro Lys Pro Asn Lys Pro Lys Asn Gly 
                325                 330                 335     
Glu Leu Thr Ile Thr Lys Thr Trp Ala Asp Ala Lys Asp Ala Pro Ile 
            340                 345                 350         
Ala Gly Val Glu Val Thr Phe Asp Leu Val Asn Ala Gln Thr Gly Glu 
        355                 360                 365             
Val Val Lys Val Pro Gly His Glu Thr Gly Ile Val Leu Asn Gln Thr 
    370                 375                 380                 
Asn Asn Trp Thr Phe Thr Ala Thr Gly Leu Asp Asn Asn Thr Glu Tyr 
385                 390                 395                 400 
Lys Phe Val Glu Arg Thr Ile Lys Gly Tyr Ser Ala Asp Tyr Gln Thr 
                405                 410                 415     
Ile Thr Glu Thr Gly Lys Ile Ala Val Lys Asn Trp Lys Asp Glu Asn 
            420                 425                 430         
Pro Glu Pro Ile Asn Pro Glu Glu Pro Arg Val Lys Thr Tyr Gly Lys 
        435                 440                 445             
Lys Phe Val Lys Val Asp Gln Lys Asp Glu Arg Leu Lys Glu Ala Gln 
    450                 455                 460                 
Phe Val Val Lys Asn Glu Gln Gly Lys Tyr Leu Ala Leu Lys Ser Ala 
465                 470                 475                 480 
Ala Gln Gln Ala Val Asn Glu Lys Ala Ala Ala Glu Ala Lys Gln Ala 
                485                 490                 495     
Leu Asp Ala Ala Ile Ala Ala Tyr Thr Asn Ala Ala Asp Lys Asn Ala 
            500                 505                 510         
Ala Gln Ala Val Val Asp Ala Ala Gln Lys Thr Tyr Asn Asp Asn Tyr 
        515                 520                 525             
Arg Ala Ala Arg Phe Gly Tyr Val Glu Val Glu Arg Lys Glu Asp Ala 
    530                 535                 540                 
Leu Val Leu Thr Ser Asn Thr Asp Gly Gln Phe Gln Ile Ser Gly Leu 
545                 550                 555                 560 
Ala Ala Gly Ser Tyr Thr Leu Glu Glu Thr Lys Ala Pro Glu Gly Phe 
                565                 570                 575     
Ala Lys Leu Gly Asp Val Lys Phe Glu Val Gly Ala Gly Ser Trp Asn 
            580                 585                 590         
Gln Gly Asp Phe Asn Tyr Leu Lys Asp Val Gln Lys Asn Asp Ala Thr 
        595                 600                 605             
Lys Val Val Asn Lys Lys Ile Thr Ile Pro Gln Thr Gly Gly Ile Gly 
    610                 615                 620                 
Thr Ile Ile Phe Ala Val Ala Gly Ala Val Ile Met Gly Ile Ala Val 
625                 630                 635                 640 
Tyr Ala Tyr Val Lys Asn Asn Lys Asp Glu Asp Gln Leu Ala 
                645                 650                 

<210> 85
<211> 890
<212> PRT
<213> Streptococcus agalactiae 2603V/R

<220> 
<223> GBS104/SAG0649

<400> 85
Met Lys Lys Arg Gln Lys Ile Trp Arg Gly Leu Ser Val Thr Leu Leu 
1               5                   10                  15      
Ile Leu Ser Gln Ile Pro Phe Gly Ile Leu Val Gln Gly Glu Thr Gln 
            20                  25                  30          
Asp Thr Asn Gln Ala Leu Gly Lys Val Ile Val Lys Lys Thr Gly Asp 
        35                  40                  45              
Asn Ala Thr Pro Leu Gly Lys Ala Thr Phe Val Leu Lys Asn Asp Asn 
    50                  55                  60                  
Asp Lys Ser Glu Thr Ser His Glu Thr Val Glu Gly Ser Gly Glu Ala 
65                  70                  75                  80  
Thr Phe Glu Asn Ile Lys Pro Gly Asp Tyr Thr Leu Arg Glu Glu Thr 
                85                  90                  95      
Ala Pro Ile Gly Tyr Lys Lys Thr Asp Lys Thr Trp Lys Val Lys Val 
            100                 105                 110         
Ala Asp Asn Gly Ala Thr Ile Ile Glu Gly Met Asp Ala Asp Lys Ala 
        115                 120                 125             
Glu Lys Arg Lys Glu Val Leu Asn Ala Gln Tyr Pro Lys Ser Ala Ile 
    130                 135                 140                 
Tyr Glu Asp Thr Lys Glu Asn Tyr Pro Leu Val Asn Val Glu Gly Ser 
145                 150                 155                 160 
Lys Val Gly Glu Gln Tyr Lys Ala Leu Asn Pro Ile Asn Gly Lys Asp 
                165                 170                 175     
Gly Arg Arg Glu Ile Ala Glu Gly Trp Leu Ser Lys Lys Ile Thr Gly 
            180                 185                 190         
Val Asn Asp Leu Asp Lys Asn Lys Tyr Lys Ile Glu Leu Thr Val Glu 
        195                 200                 205             
Gly Lys Thr Thr Val Glu Thr Lys Glu Leu Asn Gln Pro Leu Asp Val 
    210                 215                 220                 
Val Val Leu Leu Asp Asn Ser Asn Ser Met Asn Asn Glu Arg Ala Asn 
225                 230                 235                 240 
Asn Ser Gln Arg Ala Leu Lys Ala Gly Glu Ala Val Glu Lys Leu Ile 
                245                 250                 255     
Asp Lys Ile Thr Ser Asn Lys Asp Asn Arg Val Ala Leu Val Thr Tyr 
            260                 265                 270         
Ala Ser Thr Ile Phe Asp Gly Thr Glu Ala Thr Val Ser Lys Gly Val 
        275                 280                 285             
Ala Asp Gln Asn Gly Lys Ala Leu Asn Asp Ser Val Ser Trp Asp Tyr 
    290                 295                 300                 
His Lys Thr Thr Phe Thr Ala Thr Thr His Asn Tyr Ser Tyr Leu Asn 
305                 310                 315                 320 
Leu Thr Asn Asp Ala Asn Glu Val Asn Ile Leu Lys Ser Arg Ile Pro 
                325                 330                 335     
Lys Glu Ala Glu His Ile Asn Gly Asp Arg Thr Leu Tyr Gln Phe Gly 
            340                 345                 350         
Ala Thr Phe Thr Gln Lys Ala Leu Met Lys Ala Asn Glu Ile Leu Glu 
        355                 360                 365             
Thr Gln Ser Ser Asn Ala Arg Lys Lys Leu Ile Phe His Val Thr Asp 
    370                 375                 380                 
Gly Val Pro Thr Met Ser Tyr Ala Ile Asn Phe Asn Pro Tyr Ile Ser 
385                 390                 395                 400 
Thr Ser Tyr Gln Asn Gln Phe Asn Ser Phe Leu Asn Lys Ile Pro Asp 
                405                 410                 415     
Arg Ser Gly Ile Leu Gln Glu Asp Phe Ile Ile Asn Gly Asp Asp Tyr 
            420                 425                 430         
Gln Ile Val Lys Gly Asp Gly Glu Ser Phe Lys Leu Phe Ser Asp Arg 
        435                 440                 445             
Lys Val Pro Val Thr Gly Gly Thr Thr Gln Ala Ala Tyr Arg Val Pro 
    450                 455                 460                 
Gln Asn Gln Leu Ser Val Met Ser Asn Glu Gly Tyr Ala Ile Asn Ser 
465                 470                 475                 480 
Gly Tyr Ile Tyr Leu Tyr Trp Arg Asp Tyr Asn Trp Val Tyr Pro Phe 
                485                 490                 495     
Asp Pro Lys Thr Lys Lys Val Ser Ala Thr Lys Gln Ile Lys Thr His 
            500                 505                 510         
Gly Glu Pro Thr Thr Leu Tyr Phe Asn Gly Asn Ile Arg Pro Lys Gly 
        515                 520                 525             
Tyr Asp Ile Phe Thr Val Gly Ile Gly Val Asn Gly Asp Pro Gly Ala 
    530                 535                 540                 
Thr Pro Leu Glu Ala Glu Lys Phe Met Gln Ser Ile Ser Ser Lys Thr 
545                 550                 555                 560 
Glu Asn Tyr Thr Asn Val Asp Asp Thr Asn Lys Ile Tyr Asp Glu Leu 
                565                 570                 575     
Asn Lys Tyr Phe Lys Thr Ile Val Glu Glu Lys His Ser Ile Val Asp 
            580                 585                 590         
Gly Asn Val Thr Asp Pro Met Gly Glu Met Ile Glu Phe Gln Leu Lys 
        595                 600                 605             
Asn Gly Gln Ser Phe Thr His Asp Asp Tyr Val Leu Val Gly Asn Asp 
    610                 615                 620                 
Gly Ser Gln Leu Lys Asn Gly Val Ala Leu Gly Gly Pro Asn Ser Asp 
625                 630                 635                 640 
Gly Gly Ile Leu Lys Asp Val Thr Val Thr Tyr Asp Lys Thr Ser Gln 
                645                 650                 655     
Thr Ile Lys Ile Asn His Leu Asn Leu Gly Ser Gly Gln Lys Val Val 
            660                 665                 670         
Leu Thr Tyr Asp Val Arg Leu Lys Asp Asn Tyr Ile Ser Asn Lys Phe 
        675                 680                 685             
Tyr Asn Thr Asn Asn Arg Thr Thr Leu Ser Pro Lys Ser Glu Lys Glu 
    690                 695                 700                 
Pro Asn Thr Ile Arg Asp Phe Pro Ile Pro Lys Ile Arg Asp Val Arg 
705                 710                 715                 720 
Glu Phe Pro Val Leu Thr Ile Ser Asn Gln Lys Lys Met Gly Glu Val 
                725                 730                 735     
Glu Phe Ile Lys Val Asn Lys Asp Lys His Ser Glu Ser Leu Leu Gly 
            740                 745                 750         
Ala Lys Phe Gln Leu Gln Ile Glu Lys Asp Phe Ser Gly Tyr Lys Gln 
        755                 760                 765             
Phe Val Pro Glu Gly Ser Asp Val Thr Thr Lys Asn Asp Gly Lys Ile 
    770                 775                 780                 
Tyr Phe Lys Ala Leu Gln Asp Gly Asn Tyr Lys Leu Tyr Glu Ile Ser 
785                 790                 795                 800 
Ser Pro Asp Gly Tyr Ile Glu Val Lys Thr Lys Pro Val Val Thr Phe 
                805                 810                 815     
Thr Ile Gln Asn Gly Glu Val Thr Asn Leu Lys Ala Asp Pro Asn Ala 
            820                 825                 830         
Asn Lys Asn Gln Ile Gly Tyr Leu Glu Gly Asn Gly Lys His Leu Ile 
        835                 840                 845             
Thr Asn Thr Pro Lys Arg Pro Pro Gly Val Phe Pro Lys Thr Gly Gly 
    850                 855                 860                 
Ile Gly Thr Ile Val Tyr Ile Leu Val Gly Ser Thr Phe Met Ile Leu 
865                 870                 875                 880 
Thr Ile Cys Ser Phe Arg Arg Lys Gln Leu 
                885                 890 

<210> 86
<211> 901
<212> PRT
<213> Streptococcus agalactiae 2603V/R

<220> 
<223> GBS67

<400> 86
Met Arg Lys Tyr Gln Lys Phe Ser Lys Ile Leu Thr Leu Ser Leu Phe 
1               5                   10                  15      
Cys Leu Ser Gln Ile Pro Leu Asn Thr Asn Val Leu Gly Glu Ser Thr 
            20                  25                  30          
Val Pro Glu Asn Gly Ala Lys Gly Lys Leu Val Val Lys Lys Thr Asp 
        35                  40                  45              
Asp Gln Asn Lys Pro Leu Ser Lys Ala Thr Phe Val Leu Lys Thr Thr 
    50                  55                  60                  
Ala His Pro Glu Ser Lys Ile Glu Lys Val Thr Ala Glu Leu Thr Gly 
65                  70                  75                  80  
Glu Ala Thr Phe Asp Asn Leu Ile Pro Gly Asp Tyr Thr Leu Ser Glu 
                85                  90                  95      
Glu Thr Ala Pro Glu Gly Tyr Lys Lys Thr Asn Gln Thr Trp Gln Val 
            100                 105                 110         
Lys Val Glu Ser Asn Gly Lys Thr Thr Ile Gln Asn Ser Gly Asp Lys 
        115                 120                 125             
Asn Ser Thr Ile Gly Gln Asn Gln Glu Glu Leu Asp Lys Gln Tyr Pro 
    130                 135                 140                 
Pro Thr Gly Ile Tyr Glu Asp Thr Lys Glu Ser Tyr Lys Leu Glu His 
145                 150                 155                 160 
Val Lys Gly Ser Val Pro Asn Gly Lys Ser Glu Ala Lys Ala Val Asn 
                165                 170                 175     
Pro Tyr Ser Ser Glu Gly Glu His Ile Arg Glu Ile Pro Glu Gly Thr 
            180                 185                 190         
Leu Ser Lys Arg Ile Ser Glu Val Gly Asp Leu Ala His Asn Lys Tyr 
        195                 200                 205             
Lys Ile Glu Leu Thr Val Ser Gly Lys Thr Ile Val Lys Pro Val Asp 
    210                 215                 220                 
Lys Gln Lys Pro Leu Asp Val Val Phe Val Leu Asp Asn Ser Asn Ser 
225                 230                 235                 240 
Met Asn Asn Asp Gly Pro Asn Phe Gln Arg His Asn Lys Ala Lys Lys 
                245                 250                 255     
Ala Ala Glu Ala Leu Gly Thr Ala Val Lys Asp Ile Leu Gly Ala Asn 
            260                 265                 270         
Ser Asp Asn Arg Val Ala Leu Val Thr Tyr Gly Ser Asp Ile Phe Asp 
        275                 280                 285             
Gly Arg Ser Val Asp Val Val Lys Gly Phe Lys Glu Asp Asp Lys Tyr 
    290                 295                 300                 
Tyr Gly Leu Gln Thr Lys Phe Thr Ile Gln Thr Glu Asn Tyr Ser His 
305                 310                 315                 320 
Lys Gln Leu Thr Asn Asn Ala Glu Glu Ile Ile Lys Arg Ile Pro Thr 
                325                 330                 335     
Glu Ala Pro Lys Ala Lys Trp Gly Ser Thr Thr Asn Gly Leu Thr Pro 
            340                 345                 350         
Glu Gln Gln Lys Glu Tyr Tyr Leu Ser Lys Val Gly Glu Thr Phe Thr 
        355                 360                 365             
Met Lys Ala Phe Met Glu Ala Asp Asp Ile Leu Ser Gln Val Asn Arg 
    370                 375                 380                 
Asn Ser Gln Lys Ile Ile Val His Val Thr Asp Gly Val Pro Thr Arg 
385                 390                 395                 400 
Ser Tyr Ala Ile Asn Asn Phe Lys Leu Gly Ala Ser Tyr Glu Ser Gln 
                405                 410                 415     
Phe Glu Gln Met Lys Lys Asn Gly Tyr Leu Asn Lys Ser Asn Phe Leu 
            420                 425                 430         
Leu Thr Asp Lys Pro Glu Asp Ile Lys Gly Asn Gly Glu Ser Tyr Phe 
        435                 440                 445             
Leu Phe Pro Leu Asp Ser Tyr Gln Thr Gln Ile Ile Ser Gly Asn Leu 
    450                 455                 460                 
Gln Lys Leu His Tyr Leu Asp Leu Asn Leu Asn Tyr Pro Lys Gly Thr 
465                 470                 475                 480 
Ile Tyr Arg Asn Gly Pro Val Lys Glu His Gly Thr Pro Thr Lys Leu 
                485                 490                 495     
Tyr Ile Asn Ser Leu Lys Gln Lys Asn Tyr Asp Ile Phe Asn Phe Gly 
            500                 505                 510         
Ile Asp Ile Ser Gly Phe Arg Gln Val Tyr Asn Glu Glu Tyr Lys Lys 
        515                 520                 525             
Asn Gln Asp Gly Thr Phe Gln Lys Leu Lys Glu Glu Ala Phe Lys Leu 
    530                 535                 540                 
Ser Asp Gly Glu Ile Thr Glu Leu Met Arg Ser Phe Ser Ser Lys Pro 
545                 550                 555                 560 
Glu Tyr Tyr Thr Pro Ile Val Thr Ser Ala Asp Thr Ser Asn Asn Glu 
                565                 570                 575     
Ile Leu Ser Lys Ile Gln Gln Gln Phe Glu Thr Ile Leu Thr Lys Glu 
            580                 585                 590         
Asn Ser Ile Val Asn Gly Thr Ile Glu Asp Pro Met Gly Asp Lys Ile 
        595                 600                 605             
Asn Leu Gln Leu Gly Asn Gly Gln Thr Leu Gln Pro Ser Asp Tyr Thr 
    610                 615                 620                 
Leu Gln Gly Asn Asp Gly Ser Val Met Lys Asp Gly Ile Ala Thr Gly 
625                 630                 635                 640 
Gly Pro Asn Asn Asp Gly Gly Ile Leu Lys Gly Val Lys Leu Glu Tyr 
                645                 650                 655     
Ile Gly Asn Lys Leu Tyr Val Arg Gly Leu Asn Leu Gly Glu Gly Gln 
            660                 665                 670         
Lys Val Thr Leu Thr Tyr Asp Val Lys Leu Asp Asp Ser Phe Ile Ser 
        675                 680                 685             
Asn Lys Phe Tyr Asp Thr Asn Gly Arg Thr Thr Leu Asn Pro Lys Ser 
    690                 695                 700                 
Glu Asp Pro Asn Thr Leu Arg Asp Phe Pro Ile Pro Lys Ile Arg Asp 
705                 710                 715                 720 
Val Arg Glu Tyr Pro Thr Ile Thr Ile Lys Asn Glu Lys Lys Leu Gly 
                725                 730                 735     
Glu Ile Glu Phe Ile Lys Val Asp Lys Asp Asn Asn Lys Leu Leu Leu 
            740                 745                 750         
Lys Gly Ala Thr Phe Glu Leu Gln Glu Phe Asn Glu Asp Tyr Lys Leu 
        755                 760                 765             
Tyr Leu Pro Ile Lys Asn Asn Asn Ser Lys Val Val Thr Gly Glu Asn 
    770                 775                 780                 
Gly Lys Ile Ser Tyr Lys Asp Leu Lys Asp Gly Lys Tyr Gln Leu Ile 
785                 790                 795                 800 
Glu Ala Val Ser Pro Glu Asp Tyr Gln Lys Ile Thr Asn Lys Pro Ile 
                805                 810                 815     
Leu Thr Phe Glu Val Val Lys Gly Ser Ile Lys Asn Ile Ile Ala Val 
            820                 825                 830         
Asn Lys Gln Ile Ser Glu Tyr His Glu Glu Gly Asp Lys His Leu Ile 
        835                 840                 845             
Thr Asn Thr His Ile Pro Pro Lys Gly Ile Ile Pro Met Thr Gly Gly 
    850                 855                 860                 
Lys Gly Ile Leu Ser Phe Ile Leu Ile Gly Gly Ala Met Met Ser Ile 
865                 870                 875                 880 
Ala Gly Gly Ile Tyr Ile Trp Lys Arg Tyr Lys Lys Ser Ser Asp Met 
                885                 890                 895     
Ser Ile Lys Lys Asp 
            900     

<210> 87
<211> 896
<212> PRT
<213> Streptococcus agalactiae H36B

<220> 
<223> GBS67

<400> 87
Met Arg Lys Tyr Gln Lys Phe Ser Lys Ile Leu Thr Leu Ser Leu Phe 
1               5                   10                  15      
Cys Leu Ser Gln Ile Pro Leu Asn Thr Asn Val Leu Gly Glu Ser Thr 
            20                  25                  30          
Val Pro Glu Asn Gly Ala Lys Gly Lys Leu Val Val Lys Lys Thr Asp 
        35                  40                  45              
Asp Gln Asn Lys Pro Leu Ser Lys Ala Thr Phe Val Leu Lys Pro Thr 
    50                  55                  60                  
Ser His Ser Glu Ser Lys Val Glu Lys Val Thr Thr Glu Val Thr Gly 
65                  70                  75                  80  
Glu Ala Thr Phe Asp Asn Leu Thr Pro Gly Asp Tyr Thr Leu Ser Glu 
                85                  90                  95      
Glu Thr Ala Pro Glu Gly Tyr Lys Lys Thr Thr Gln Thr Trp Gln Val 
            100                 105                 110         
Lys Val Glu Ser Asn Gly Lys Thr Thr Ile Gln Asn Ser Asp Asp Lys 
        115                 120                 125             
Lys Ser Ile Ile Glu Gln Arg Gln Glu Glu Leu Asp Lys Gln Tyr Pro 
    130                 135                 140                 
Leu Thr Gly Ala Tyr Glu Asp Thr Lys Glu Ser Tyr Asn Leu Glu His 
145                 150                 155                 160 
Val Lys Asn Ser Ile Pro Asn Gly Lys Leu Glu Ala Lys Ala Val Asn 
                165                 170                 175     
Pro Tyr Ser Ser Glu Gly Glu His Ile Arg Glu Ile Gln Glu Gly Thr 
            180                 185                 190         
Leu Ser Lys Arg Ile Ser Glu Val Asn Asp Leu Asp His Asn Lys Tyr 
        195                 200                 205             
Lys Ile Glu Leu Thr Val Ser Gly Lys Ser Ile Ile Lys Thr Ile Asn 
    210                 215                 220                 
Lys Asp Glu Pro Leu Asp Val Val Phe Val Leu Asp Asn Ser Asn Ser 
225                 230                 235                 240 
Met Lys Asn Asn Gly Lys Asn Asn Lys Ala Lys Lys Ala Gly Glu Ala 
                245                 250                 255     
Val Glu Thr Ile Ile Lys Asp Val Leu Gly Ala Asn Val Glu Asn Arg 
            260                 265                 270         
Ala Ala Leu Val Thr Tyr Gly Ser Asp Ile Phe Asp Gly Arg Thr Val 
        275                 280                 285             
Lys Val Ile Lys Gly Phe Lys Glu Asp Pro Tyr Tyr Gly Leu Glu Thr 
    290                 295                 300                 
Ser Phe Thr Val Gln Thr Asn Asp Tyr Ser Tyr Lys Lys Phe Thr Asn 
305                 310                 315                 320 
Ile Ala Ala Asp Ile Ile Lys Lys Ile Pro Lys Glu Ala Pro Glu Ala 
                325                 330                 335     
Lys Trp Gly Gly Thr Ser Leu Gly Leu Thr Pro Glu Lys Lys Arg Glu 
            340                 345                 350         
Tyr Asp Leu Ser Lys Val Gly Glu Thr Phe Thr Met Lys Ala Phe Met 
        355                 360                 365             
Glu Ala Asp Thr Leu Leu Ser Ser Ile Gln Arg Lys Ser Arg Lys Ile 
    370                 375                 380                 
Ile Val His Leu Thr Asp Gly Val Pro Thr Arg Ser Tyr Ala Ile Asn 
385                 390                 395                 400 
Ser Phe Val Lys Gly Ser Thr Tyr Ala Asn Gln Phe Glu Arg Ile Lys 
                405                 410                 415     
Glu Lys Gly Tyr Leu Asp Lys Asn Asn Tyr Phe Ile Thr Asp Asp Pro 
            420                 425                 430         
Glu Lys Ile Lys Gly Asn Gly Glu Ser Tyr Phe Leu Phe Pro Leu Asp 
        435                 440                 445             
Ser Tyr Gln Thr Gln Ile Ile Ser Gly Asn Leu Gln Lys Leu His Tyr 
    450                 455                 460                 
Leu Asp Leu Asn Leu Asn Tyr Pro Lys Gly Thr Ile Tyr Arg Asn Gly 
465                 470                 475                 480 
Pro Val Arg Glu His Gly Thr Pro Thr Lys Leu Tyr Ile Asn Ser Leu 
                485                 490                 495     
Lys Gln Lys Asn Tyr Asp Ile Phe Asn Phe Gly Ile Asp Ile Ser Gly 
            500                 505                 510         
Phe Arg Gln Val Tyr Asn Glu Asp Tyr Lys Lys Asn Gln Asp Gly Thr 
        515                 520                 525             
Phe Gln Lys Leu Lys Glu Glu Ala Phe Glu Leu Ser Asp Gly Glu Ile 
    530                 535                 540                 
Thr Glu Leu Met Asn Ser Phe Ser Ser Lys Pro Glu Tyr Tyr Thr Pro 
545                 550                 555                 560 
Ile Val Thr Ser Ala Asp Val Ser Asn Asn Glu Ile Leu Ser Lys Ile 
                565                 570                 575     
Gln Gln Gln Phe Glu Lys Ile Leu Thr Lys Glu Asn Ser Ile Val Asn 
            580                 585                 590         
Gly Thr Ile Glu Asp Pro Met Gly Asp Lys Ile Asn Leu His Leu Gly 
        595                 600                 605             
Asn Gly Gln Thr Leu Gln Pro Ser Asp Tyr Thr Leu Gln Gly Asn Asp 
    610                 615                 620                 
Gly Ser Ile Met Lys Asp Ser Ile Ala Thr Gly Gly Pro Asn Asn Asp 
625                 630                 635                 640 
Gly Gly Ile Leu Lys Gly Val Lys Leu Glu Tyr Ile Lys Asn Lys Leu 
                645                 650                 655     
Tyr Val Arg Gly Leu Asn Leu Gly Glu Gly Gln Lys Val Thr Leu Thr 
            660                 665                 670         
Tyr Asp Val Lys Leu Asp Asp Ser Phe Ile Ser Asn Lys Phe Tyr Asp 
        675                 680                 685             
Thr Asn Gly Arg Thr Thr Leu Asn Pro Lys Ser Glu Glu Pro Asp Thr 
    690                 695                 700                 
Leu Arg Asp Phe Pro Ile Pro Lys Ile Arg Asp Val Arg Glu Tyr Pro 
705                 710                 715                 720 
Thr Ile Thr Ile Lys Asn Glu Lys Lys Leu Gly Glu Ile Glu Phe Thr 
                725                 730                 735     
Lys Val Asp Lys Asp Asn Asn Lys Leu Leu Leu Lys Gly Ala Thr Phe 
            740                 745                 750         
Glu Leu Gln Glu Phe Asn Glu Asp Tyr Lys Leu Tyr Leu Pro Ile Lys 
        755                 760                 765             
Asn Asn Asn Ser Lys Val Val Thr Gly Glu Asn Gly Lys Ile Ser Tyr 
    770                 775                 780                 
Lys Asp Leu Lys Asp Gly Lys Tyr Gln Leu Ile Glu Ala Val Ser Pro 
785                 790                 795                 800 
Lys Asp Tyr Gln Lys Ile Thr Asn Lys Pro Ile Leu Thr Phe Glu Val 
                805                 810                 815     
Val Lys Gly Ser Ile Gln Asn Ile Ile Ala Val Asn Lys Gln Ile Ser 
            820                 825                 830         
Glu Tyr His Glu Glu Gly Asp Lys His Leu Ile Thr Asn Thr His Ile 
        835                 840                 845             
Pro Pro Lys Gly Ile Ile Pro Met Thr Gly Gly Lys Gly Ile Leu Ser 
    850                 855                 860                 
Phe Ile Leu Ile Gly Gly Ala Met Met Ser Ile Ala Gly Gly Ile Tyr 
865                 870                 875                 880 
Ile Trp Lys Arg His Lys Lys Ser Ser Asp Ala Ser Ile Glu Lys Asp 
                885                 890                 895     



<210> 88
<211> 901
<212> PRT
<213> Streptococcus agalactiae CJB111

<220> 
<223> GBS67

<400> 88
Met Arg Lys Tyr Gln Lys Phe Ser Lys Ile Leu Thr Leu Ser Leu Phe 
1               5                   10                  15      
Cys Leu Ser Gln Ile Pro Leu Asn Thr Asn Val Leu Gly Glu Ser Thr 
            20                  25                  30          
Val Pro Glu Asn Gly Ala Lys Gly Lys Leu Val Val Lys Lys Thr Asp 
        35                  40                  45              
Asp Gln Asn Lys Pro Leu Ser Lys Ala Thr Phe Val Leu Lys Thr Thr 
    50                  55                  60                  
Ala His Pro Glu Ser Lys Ile Glu Lys Val Thr Ala Glu Leu Thr Gly 
65                  70                  75                  80  
Glu Ala Thr Phe Asp Asn Leu Ile Pro Gly Asp Tyr Thr Leu Ser Glu 
                85                  90                  95      
Glu Thr Ala Pro Glu Gly Tyr Lys Lys Thr Asn Gln Thr Trp Gln Val 
            100                 105                 110         
Lys Val Glu Ser Asn Gly Lys Thr Thr Ile Gln Asn Ser Gly Asp Lys 
        115                 120                 125             
Asn Ser Thr Ile Gly Gln Asn Gln Glu Glu Leu Asp Lys Gln Tyr Pro 
    130                 135                 140                 
Pro Thr Gly Ile Tyr Glu Asp Thr Lys Glu Ser Tyr Lys Leu Glu His 
145                 150                 155                 160 
Val Lys Gly Ser Val Pro Asn Gly Lys Ser Glu Ala Lys Ala Val Asn 
                165                 170                 175     
Pro Tyr Ser Ser Glu Gly Glu His Ile Arg Glu Ile Pro Glu Gly Thr 
            180                 185                 190         
Leu Ser Lys Arg Ile Ser Glu Val Gly Asp Leu Ala His Asn Lys Tyr 
        195                 200                 205             
Lys Ile Glu Leu Thr Val Ser Gly Lys Thr Ile Val Lys Pro Val Asp 
    210                 215                 220                 
Lys Gln Lys Pro Leu Asp Val Val Phe Val Leu Asp Asn Ser Asn Ser 
225                 230                 235                 240 
Met Asn Asn Asp Gly Pro Asn Phe Gln Arg His Asn Lys Ala Lys Lys 
                245                 250                 255     
Ala Ala Glu Ala Leu Gly Thr Ala Val Lys Asp Ile Leu Gly Ala Asn 
            260                 265                 270         
Ser Asp Asn Arg Val Ala Leu Val Thr Tyr Gly Ser Asp Ile Phe Asp 
        275                 280                 285             
Gly Arg Ser Val Asp Val Val Lys Gly Phe Lys Glu Asp Asp Lys Tyr 
    290                 295                 300                 
Tyr Gly Leu Gln Thr Lys Phe Thr Ile Gln Thr Glu Asn Tyr Ser His 
305                 310                 315                 320 
Lys Gln Leu Thr Asn Asn Ala Glu Glu Ile Ile Lys Arg Ile Pro Thr 
                325                 330                 335     
Glu Ala Pro Lys Ala Lys Trp Gly Ser Thr Thr Asn Gly Leu Thr Pro 
            340                 345                 350         
Glu Gln Gln Lys Glu Tyr Tyr Leu Ser Lys Val Gly Glu Thr Phe Thr 
        355                 360                 365             
Met Lys Ala Phe Met Glu Ala Asp Asp Ile Leu Ser Gln Val Asn Arg 
    370                 375                 380                 
Asn Ser Gln Lys Ile Ile Val His Val Thr Asp Gly Val Pro Thr Arg 
385                 390                 395                 400 
Ser Tyr Ala Ile Asn Asn Phe Lys Leu Gly Ala Ser Tyr Glu Ser Gln 
                405                 410                 415     
Phe Glu Gln Met Lys Lys Asn Gly Tyr Leu Asn Lys Ser Asn Phe Leu 
            420                 425                 430         
Leu Thr Asp Lys Pro Glu Asp Ile Lys Gly Asn Gly Glu Ser Tyr Phe 
        435                 440                 445             
Leu Phe Pro Leu Asp Ser Tyr Gln Thr Gln Ile Ile Ser Gly Asn Leu 
    450                 455                 460                 
Gln Lys Leu His Tyr Leu Asp Leu Asn Leu Asn Tyr Pro Lys Gly Thr 
465                 470                 475                 480 
Phe Tyr Arg Asn Gly Pro Val Arg Glu His Gly Thr Pro Thr Lys Leu 
                485                 490                 495     
Tyr Ile Asn Ser Leu Lys Gln Lys Asn Tyr Asp Ile Phe Asn Phe Gly 
            500                 505                 510         
Ile Asp Ile Ser Gly Phe Arg Gln Val Tyr Asn Glu Asp Tyr Lys Lys 
        515                 520                 525             
Asn Gln Asp Gly Thr Phe Gln Lys Leu Lys Glu Glu Ala Phe Glu Leu 
    530                 535                 540                 
Ser Asp Gly Glu Ile Thr Glu Leu Met Lys Ser Phe Ser Ser Lys Pro 
545                 550                 555                 560 
Glu Tyr Tyr Thr Pro Ile Val Thr Ser Ser Asp Ala Ser Asn Asn Glu 
                565                 570                 575     
Ile Leu Ser Lys Ile Gln Gln Gln Phe Glu Lys Ile Leu Thr Lys Glu 
            580                 585                 590         
Asn Ser Ile Val Asn Gly Thr Ile Glu Asp Pro Met Gly Asp Lys Ile 
        595                 600                 605             
Asn Leu Gln Leu Gly Asn Gly Gln Thr Leu Gln Pro Ser Asp Tyr Thr 
    610                 615                 620                 
Leu Gln Gly Asn Asp Gly Ser Ile Met Lys Asp Ser Ile Ala Thr Gly 
625                 630                 635                 640 
Gly Pro Asn Asn Asp Gly Gly Ile Leu Lys Gly Val Lys Leu Glu Tyr 
                645                 650                 655     
Ile Lys Asn Lys Leu Tyr Val Arg Gly Leu Asn Leu Gly Glu Gly Gln 
            660                 665                 670         
Lys Val Thr Leu Thr Tyr Asp Val Lys Leu Asp Asp Ser Phe Ile Ser 
        675                 680                 685             
Asn Lys Phe Tyr Asp Thr Asn Gly Arg Thr Thr Leu Asn Pro Lys Ser 
    690                 695                 700                 
Glu Asp Pro Asn Thr Leu Arg Asp Phe Pro Ile Pro Lys Ile Arg Asp 
705                 710                 715                 720 
Val Arg Glu Tyr Pro Thr Ile Thr Ile Lys Asn Glu Lys Lys Leu Gly 
                725                 730                 735     
Glu Ile Glu Phe Thr Lys Val Asp Lys Asp Asn Asn Lys Leu Leu Leu 
            740                 745                 750         
Lys Gly Ala Thr Phe Glu Leu Gln Glu Phe Asn Glu Asp Tyr Lys Leu 
        755                 760                 765             
Tyr Leu Pro Ile Lys Asn Asn Asn Ser Lys Val Val Thr Gly Glu Asn 
    770                 775                 780                 
Gly Lys Ile Ser Tyr Lys Asp Leu Lys Asp Gly Lys Tyr Gln Leu Ile 
785                 790                 795                 800 
Glu Ala Val Ser Pro Lys Asp Tyr Gln Lys Ile Thr Asn Lys Pro Ile 
                805                 810                 815     
Leu Thr Phe Glu Val Val Lys Gly Ser Ile Gln Asn Ile Ile Ala Val 
            820                 825                 830         
Asn Lys Gln Ile Ser Glu Tyr His Glu Glu Gly Asp Lys His Leu Ile 
        835                 840                 845             
Thr Asn Thr His Ile Pro Pro Lys Gly Ile Ile Pro Met Thr Gly Gly 
    850                 855                 860                 
Lys Gly Ile Leu Ser Phe Ile Leu Ile Gly Gly Ser Met Met Ser Ile 
865                 870                 875                 880 
Ala Gly Gly Ile Tyr Ile Trp Lys Arg Tyr Lys Lys Ser Ser Asp Ile 
                885                 890                 895     
Ser Arg Glu Lys Asp 
            900     

<210> 89
<211> 901
<212> PRT
<213> Streptococcus agalactiae 515

<220> 
<223> GBS67

<400> 89
Met Arg Lys Tyr Gln Lys Phe Ser Lys Ile Leu Thr Leu Ser Leu Phe 
1               5                   10                  15      
Cys Leu Ser Gln Ile Pro Leu Asn Thr Asn Val Leu Gly Glu Ser Thr 
            20                  25                  30          
Val Pro Glu Asn Gly Ala Lys Gly Lys Leu Val Val Lys Lys Thr Asp 
        35                  40                  45              
Asp Gln Asn Lys Pro Leu Ser Lys Ala Thr Phe Val Leu Lys Thr Thr 
    50                  55                  60                  
Ala His Pro Glu Ser Lys Ile Glu Lys Val Thr Ala Glu Leu Thr Gly 
65                  70                  75                  80  
Glu Ala Thr Phe Asp Asn Leu Ile Pro Gly Asp Tyr Thr Leu Ser Glu 
                85                  90                  95      
Glu Thr Ala Pro Glu Gly Tyr Lys Lys Thr Asn Gln Thr Trp Gln Val 
            100                 105                 110         
Lys Val Glu Ser Asn Gly Lys Thr Thr Ile Gln Asn Ser Gly Asp Lys 
        115                 120                 125             
Asn Ser Thr Ile Gly Gln Asn Gln Glu Glu Leu Asp Lys Gln Tyr Pro 
    130                 135                 140                 
Pro Thr Gly Ile Tyr Glu Asp Thr Lys Glu Ser Tyr Lys Leu Glu His 
145                 150                 155                 160 
Val Lys Gly Ser Val Pro Asn Gly Lys Ser Glu Ala Lys Ala Val Asn 
                165                 170                 175     
Pro Tyr Ser Ser Glu Gly Glu His Ile Arg Glu Ile Pro Glu Gly Thr 
            180                 185                 190         
Leu Ser Lys Arg Ile Ser Glu Val Gly Asp Leu Ala His Asn Lys Tyr 
        195                 200                 205             
Lys Ile Glu Leu Thr Val Ser Gly Lys Thr Ile Val Lys Pro Val Asp 
    210                 215                 220                 
Lys Gln Lys Pro Leu Asp Val Val Phe Val Leu Asp Asn Ser Asn Ser 
225                 230                 235                 240 
Met Asn Asn Asp Gly Pro Asn Phe Gln Arg His Asn Lys Ala Lys Lys 
                245                 250                 255     
Ala Ala Glu Ala Leu Gly Thr Ala Val Lys Asp Ile Leu Gly Ala Asn 
            260                 265                 270         
Ser Asp Asn Arg Val Ala Leu Val Thr Tyr Gly Ser Asp Ile Phe Asp 
        275                 280                 285             
Gly Arg Ser Val Asp Val Val Lys Gly Phe Lys Glu Asp Asp Lys Tyr 
    290                 295                 300                 
Tyr Gly Leu Gln Thr Lys Phe Thr Ile Gln Thr Glu Asn Tyr Ser His 
305                 310                 315                 320 
Lys Gln Leu Thr Asn Asn Ala Glu Glu Ile Ile Lys Arg Ile Pro Thr 
                325                 330                 335     
Glu Ala Pro Lys Ala Lys Trp Gly Ser Thr Thr Asn Gly Leu Thr Pro 
            340                 345                 350         
Glu Gln Gln Lys Glu Tyr Tyr Leu Ser Lys Val Gly Glu Thr Phe Thr 
        355                 360                 365             
Met Lys Ala Phe Met Glu Ala Asp Asp Ile Leu Ser Gln Val Asn Arg 
    370                 375                 380                 
Asn Ser Gln Lys Ile Ile Val His Val Thr Asp Gly Val Pro Thr Arg 
385                 390                 395                 400 
Ser Tyr Ala Ile Asn Asn Phe Lys Leu Gly Ala Ser Tyr Glu Ser Gln 
                405                 410                 415     
Phe Glu Gln Met Lys Lys Asn Gly Tyr Leu Asn Lys Ser Asn Phe Leu 
            420                 425                 430         
Leu Thr Asp Lys Pro Asp Asp Ile Lys Gly Asn Gly Glu Ser Tyr Phe 
        435                 440                 445             
Leu Phe Pro Leu Asp Ser Tyr Gln Thr Gln Ile Ile Ser Gly Asn Leu 
    450                 455                 460                 
Gln Lys Leu His Tyr Leu Asp Leu Asn Leu Asn Tyr Pro Lys Gly Thr 
465                 470                 475                 480 
Ile Tyr Arg Asn Gly Pro Val Lys Glu His Gly Thr Pro Thr Lys Leu 
                485                 490                 495     
Tyr Ile Asn Ser Leu Lys Gln Lys Asn Tyr Asp Ile Phe Asn Phe Gly 
            500                 505                 510         
Ile Asp Ile Ser Gly Phe Arg Gln Val Tyr Asn Glu Glu Tyr Lys Lys 
        515                 520                 525             
Asn Gln Asp Gly Thr Phe Gln Lys Leu Lys Glu Glu Ala Phe Lys Leu 
    530                 535                 540                 
Ser Asp Gly Glu Ile Thr Glu Leu Met Arg Ser Phe Ser Ser Lys Pro 
545                 550                 555                 560 
Glu Tyr Tyr Thr Pro Ile Val Thr Ser Ala Asp Thr Ser Asn Asn Glu 
                565                 570                 575     
Ile Leu Ser Lys Ile Gln Gln Gln Phe Glu Thr Ile Leu Thr Lys Glu 
            580                 585                 590         
Asn Ser Ile Val Asn Gly Thr Ile Glu Asp Pro Met Gly Asp Lys Ile 
        595                 600                 605             
Asn Leu Gln Leu Gly Asn Gly Gln Ile Leu Gln Pro Ser Asp Tyr Thr 
    610                 615                 620                 
Leu Gln Gly Asn Asp Gly Ser Val Met Lys Asp Gly Ile Ala Thr Gly 
625                 630                 635                 640 
Gly Pro Asn Asn Asp Gly Gly Ile Leu Lys Gly Val Lys Leu Glu Tyr 
                645                 650                 655     
Ile Gly Asn Lys Leu Tyr Val Arg Gly Leu Asn Leu Gly Glu Gly Gln 
            660                 665                 670         
Lys Val Thr Leu Thr Tyr Asp Val Lys Leu Asp Asp Ser Phe Ile Ser 
        675                 680                 685             
Asn Lys Phe Tyr Asp Thr Asn Gly Arg Thr Thr Leu Asn Pro Lys Ser 
    690                 695                 700                 
Glu Asp Pro Asn Thr Leu Arg Asp Phe Pro Ile Pro Lys Ile Arg Asp 
705                 710                 715                 720 
Val Arg Glu Tyr Pro Thr Ile Thr Ile Lys Asn Glu Lys Lys Leu Gly 
                725                 730                 735     
Glu Ile Glu Phe Ile Lys Val Asp Lys Asp Asn Asn Lys Leu Leu Leu 
            740                 745                 750         
Lys Gly Ala Thr Phe Glu Leu Gln Glu Phe Asn Glu Asp Tyr Lys Leu 
        755                 760                 765             
Tyr Leu Pro Ile Lys Asn Asn Asn Ser Lys Val Val Thr Gly Glu Asn 
    770                 775                 780                 
Gly Lys Ile Ser Tyr Lys Asp Leu Lys Asp Gly Lys Tyr Gln Leu Ile 
785                 790                 795                 800 
Glu Ala Val Ser Pro Glu Asp Tyr Gln Lys Ile Thr Asn Lys Pro Ile 
                805                 810                 815     
Leu Thr Phe Glu Val Val Lys Gly Ser Ile Lys Asn Ile Ile Ala Val 
            820                 825                 830         
Asn Lys Gln Ile Ser Glu Tyr His Glu Glu Gly Asp Lys His Leu Ile 
        835                 840                 845             
Thr Asn Thr His Ile Pro Pro Lys Gly Ile Ile Pro Lys Thr Gly Gly 
    850                 855                 860                 
Lys Gly Ile Leu Ser Phe Ile Leu Ile Gly Gly Ala Met Met Ser Ile 
865                 870                 875                 880 
Ala Gly Gly Ile Tyr Ile Trp Lys Arg Tyr Lys Lys Ser Ser Asp Met 
                885                 890                 895     
Ser Ile Lys Lys Asp 
            900     

<210> 90
<211> 901
<212> PRT
<213> Streptococcus agalactiae NEM316

<220> 
<223> GBS67

<400> 90
Met Arg Lys Tyr Gln Lys Phe Ser Lys Ile Leu Thr Leu Ser Leu Phe 
1               5                   10                  15      
Cys Leu Ser Gln Ile Pro Leu Asn Thr Asn Val Leu Gly Glu Ser Thr 
            20                  25                  30          
Val Pro Glu Asn Gly Ala Lys Gly Lys Leu Val Val Lys Lys Thr Asp 
        35                  40                  45              
Asp Gln Asn Lys Pro Leu Ser Lys Ala Thr Phe Val Leu Lys Thr Thr 
    50                  55                  60                  
Ala His Pro Glu Ser Lys Ile Glu Lys Val Thr Ala Glu Leu Thr Gly 
65                  70                  75                  80  
Glu Ala Thr Phe Asp Asn Leu Ile Pro Gly Asp Tyr Thr Leu Ser Glu 
                85                  90                  95      
Glu Thr Ala Pro Glu Gly Tyr Lys Lys Thr Asn Gln Thr Trp Gln Val 
            100                 105                 110         
Lys Val Glu Ser Asn Gly Lys Thr Thr Ile Gln Asn Ser Gly Asp Lys 
        115                 120                 125             
Asn Ser Thr Ile Gly Gln Asn His Glu Glu Leu Asp Lys Gln Tyr Pro 
    130                 135                 140                 
Pro Thr Gly Ile Tyr Glu Asp Thr Lys Glu Ser Tyr Lys Leu Glu His 
145                 150                 155                 160 
Val Lys Gly Ser Val Pro Asn Gly Lys Ser Glu Ala Lys Ala Val Asn 
                165                 170                 175     
Pro Tyr Ser Ser Glu Gly Glu His Ile Arg Glu Ile Pro Glu Gly Thr 
            180                 185                 190         
Leu Ser Lys Arg Ile Ser Glu Val Gly Asp Leu Ala His Asn Lys Tyr 
        195                 200                 205             
Lys Ile Glu Leu Thr Val Ser Gly Lys Thr Ile Val Lys Pro Val Asp 
    210                 215                 220                 
Lys Gln Lys Pro Leu Asp Val Val Phe Val Leu Asp Asn Ser Asn Ser 
225                 230                 235                 240 
Met Asn Asn Asp Gly Pro Asn Phe Gln Arg His Asn Lys Ala Lys Lys 
                245                 250                 255     
Ala Ala Glu Ala Leu Gly Thr Ala Val Lys Asp Ile Leu Gly Ala Asn 
            260                 265                 270         
Ser Asp Asn Arg Val Ala Leu Val Thr Tyr Gly Ser Asp Ile Phe Asp 
        275                 280                 285             
Gly Arg Ser Val Asp Val Val Lys Gly Phe Lys Glu Asp Asp Lys Tyr 
    290                 295                 300                 
Tyr Gly Leu Gln Thr Lys Phe Thr Ile Gln Thr Glu Asn Tyr Ser His 
305                 310                 315                 320 
Lys Gln Leu Thr Asn Asn Ala Glu Glu Ile Ile Lys Arg Ile Pro Thr 
                325                 330                 335     
Glu Ala Pro Arg Ala Lys Trp Gly Ser Thr Thr Asn Gly Leu Thr Pro 
            340                 345                 350         
Glu Gln Gln Lys Gln Tyr Tyr Leu Ser Lys Val Gly Glu Thr Phe Thr 
        355                 360                 365             
Met Lys Ala Phe Met Glu Ala Asp Asp Ile Leu Ser Gln Val Asp Arg 
    370                 375                 380                 
Asn Ser Gln Lys Ile Ile Val His Ile Thr Asp Gly Val Pro Thr Arg 
385                 390                 395                 400 
Ser Tyr Ala Ile Asn Asn Phe Lys Leu Gly Ala Ser Tyr Glu Ser Gln 
                405                 410                 415     
Phe Glu Gln Met Lys Lys Asn Gly Tyr Leu Asn Lys Ser Asn Phe Leu 
            420                 425                 430         
Leu Thr Asp Lys Pro Glu Asp Ile Lys Gly Asn Gly Glu Ser Tyr Phe 
        435                 440                 445             
Leu Phe Pro Leu Asp Ser Tyr Gln Thr Gln Ile Ile Ser Gly Asn Leu 
    450                 455                 460                 
Gln Lys Leu His Tyr Leu Asp Leu Asn Leu Asn Tyr Pro Lys Gly Thr 
465                 470                 475                 480 
Ile Tyr Arg Asn Gly Pro Val Arg Glu His Gly Thr Pro Thr Lys Leu 
                485                 490                 495     
Tyr Ile Asn Ser Leu Lys Gln Lys Asn Tyr Asp Ile Phe Asn Phe Gly 
            500                 505                 510         
Ile Asp Ile Ser Ala Phe Arg Gln Val Tyr Asn Glu Asp Tyr Lys Lys 
        515                 520                 525             
Asn Gln Asp Gly Thr Phe Gln Lys Leu Lys Glu Glu Ala Phe Glu Leu 
    530                 535                 540                 
Ser Asp Gly Glu Ile Thr Glu Leu Met Lys Ser Phe Ser Ser Lys Pro 
545                 550                 555                 560 
Glu Tyr Tyr Thr Pro Ile Val Thr Ser Ser Asp Ala Ser Asn Asn Glu 
                565                 570                 575     
Ile Leu Ser Lys Ile Gln Gln Gln Phe Glu Lys Val Leu Thr Lys Glu 
            580                 585                 590         
Asn Ser Ile Val Asn Gly Thr Ile Glu Asp Pro Met Gly Asp Lys Ile 
        595                 600                 605             
Asn Leu Gln Leu Gly Asn Gly Gln Thr Leu Gln Pro Ser Asp Tyr Thr 
    610                 615                 620                 
Leu Gln Gly Asn Asp Gly Ser Ile Met Lys Asp Ser Ile Ala Thr Gly 
625                 630                 635                 640 
Gly Pro Asn Asn Asp Gly Gly Ile Leu Lys Gly Val Lys Leu Glu Tyr 
                645                 650                 655     
Ile Lys Asn Lys Leu Tyr Val Arg Gly Leu Asn Leu Gly Glu Gly Gln 
            660                 665                 670         
Lys Val Thr Leu Thr Tyr Asp Val Lys Leu Asp Asp Ser Phe Ile Ser 
        675                 680                 685             
Asn Lys Phe Tyr Asp Thr Asn Gly Arg Thr Thr Leu Asn Pro Lys Ser 
    690                 695                 700                 
Glu Asp Pro Asn Thr Leu Arg Asp Phe Pro Ile Pro Lys Ile Arg Asp 
705                 710                 715                 720 
Val Arg Glu Tyr Pro Thr Ile Thr Ile Lys Asn Glu Lys Lys Leu Gly 
                725                 730                 735     
Glu Ile Glu Phe Thr Lys Val Asp Lys Asp Asn Asn Lys Leu Leu Leu 
            740                 745                 750         
Lys Gly Ala Thr Phe Glu Leu Gln Glu Phe Asn Glu Asp Tyr Lys Leu 
        755                 760                 765             
Tyr Leu Pro Ile Lys Asn Asn Asn Ser Lys Val Val Thr Gly Glu Asn 
    770                 775                 780                 
Gly Lys Ile Ser Tyr Lys Asp Leu Lys Asp Gly Lys Tyr Gln Leu Ile 
785                 790                 795                 800 
Glu Ala Val Ser Pro Lys Asp Tyr Gln Lys Ile Thr Asn Lys Pro Ile 
                805                 810                 815     
Leu Thr Phe Glu Val Val Lys Gly Ser Ile Gln Asn Ile Ile Ala Val 
            820                 825                 830         
Asn Lys Gln Ile Ser Glu Tyr His Glu Glu Gly Asp Lys His Leu Ile 
        835                 840                 845             
Thr Asn Thr His Ile Pro Pro Lys Gly Ile Ile Pro Met Thr Gly Gly 
    850                 855                 860                 
Lys Gly Ile Leu Ser Phe Ile Leu Ile Gly Gly Ser Met Met Ser Ile 
865                 870                 875                 880 
Ala Gly Gly Ile Tyr Ile Trp Lys Arg Tyr Lys Lys Ser Ser Asp Ile 
                885                 890                 895     
Ser Arg Glu Lys Asp 
            900     

<210> 91
<211> 896
<212> PRT
<213> Streptococcus agalactiae DK21

<220> 
<223> GBS67

<400> 91
Met Arg Lys Tyr Gln Lys Phe Ser Lys Ile Leu Thr Leu Ser Leu Phe 
1               5                   10                  15      
Cys Leu Ser Gln Ile Pro Leu Asn Thr Asn Val Leu Gly Glu Ser Thr 
            20                  25                  30          
Val Pro Glu Asn Gly Ala Lys Gly Lys Leu Val Val Lys Lys Thr Asp 
        35                  40                  45              
Asp Gln Asn Lys Pro Leu Ser Lys Ala Thr Phe Val Leu Lys Pro Thr 
    50                  55                  60                  
Ser His Ser Glu Ser Lys Val Glu Lys Val Thr Thr Glu Val Thr Gly 
65                  70                  75                  80  
Glu Ala Thr Phe Asp Asn Leu Thr Pro Gly Asp Tyr Thr Leu Ser Glu 
                85                  90                  95      
Glu Thr Ala Pro Glu Gly Tyr Lys Lys Thr Thr Gln Thr Trp Gln Val 
            100                 105                 110         
Lys Val Glu Ser Asn Gly Lys Thr Thr Ile Gln Asn Ser Asp Asp Lys 
        115                 120                 125             
Lys Ser Ile Ile Glu Gln Arg Gln Glu Glu Leu Asp Lys Gln Tyr Pro 
    130                 135                 140                 
Leu Thr Gly Ala Tyr Glu Asp Thr Lys Glu Ser Tyr Asn Leu Glu His 
145                 150                 155                 160 
Val Lys Asn Ser Ile Pro Asn Gly Lys Leu Glu Ala Lys Ala Val Asn 
                165                 170                 175     
Pro Tyr Ser Ser Glu Gly Glu His Ile Arg Glu Ile Gln Glu Gly Thr 
            180                 185                 190         
Leu Ser Lys Arg Ile Ser Glu Val Asn Asp Leu Asp His Asn Lys Tyr 
        195                 200                 205             
Lys Ile Glu Leu Thr Val Ser Gly Lys Ser Ile Ile Lys Thr Ile Asn 
    210                 215                 220                 
Lys Asp Glu Pro Leu Asp Val Val Phe Val Leu Asp Asn Ser Asn Ser 
225                 230                 235                 240 
Met Lys Asn Asn Gly Lys Asn Asn Lys Ala Lys Lys Ala Gly Glu Ala 
                245                 250                 255     
Val Glu Thr Ile Ile Lys Asp Val Leu Gly Ala Asn Val Glu Asn Arg 
            260                 265                 270         
Ala Ala Leu Val Thr Tyr Gly Ser Asp Ile Phe Asp Gly Arg Thr Val 
        275                 280                 285             
Lys Val Ile Lys Gly Phe Lys Glu Asp Pro Tyr Tyr Gly Leu Glu Thr 
    290                 295                 300                 
Ser Phe Thr Val Gln Thr Asn Asp Tyr Ser Tyr Lys Lys Phe Thr Asn 
305                 310                 315                 320 
Ile Ala Ala Asp Ile Ile Lys Lys Ile Pro Lys Glu Ala Pro Glu Ala 
                325                 330                 335     
Lys Trp Gly Gly Thr Ser Leu Gly Leu Thr Pro Glu Lys Lys Arg Glu 
            340                 345                 350         
Tyr Asp Leu Ser Lys Val Gly Glu Thr Phe Thr Met Lys Ala Phe Met 
        355                 360                 365             
Glu Ala Asp Thr Leu Leu Ser Ser Ile Gln Arg Lys Ser Arg Lys Ile 
    370                 375                 380                 
Ile Val His Leu Thr Asp Gly Val Pro Thr Arg Ser Tyr Ala Ile Asn 
385                 390                 395                 400 
Ser Phe Val Thr Gly Ser Thr Tyr Ala Asn Gln Phe Glu Arg Ile Lys 
                405                 410                 415     
Glu Lys Gly Tyr Leu Asp Lys Asn Asn Tyr Phe Ile Thr Asp Asp Pro 
            420                 425                 430         
Glu Lys Ile Lys Gly Asn Gly Glu Ser Tyr Phe Leu Phe Pro Leu Asp 
        435                 440                 445             
Ser Tyr Gln Thr Gln Ile Ile Ser Gly Asn Leu Gln Lys Leu His Tyr 
    450                 455                 460                 
Leu Asp Leu Asn Leu Asn Tyr Pro Lys Gly Thr Ile Tyr Arg Asn Gly 
465                 470                 475                 480 
Pro Val Arg Glu His Gly Thr Pro Thr Lys Leu Tyr Ile Asn Ser Leu 
                485                 490                 495     
Lys Gln Lys Asn Tyr Asp Ile Phe Asn Phe Gly Ile Asp Ile Ser Gly 
            500                 505                 510         
Phe Arg Gln Val Tyr Asn Glu Asp Tyr Lys Lys Asn Gln Asp Gly Thr 
        515                 520                 525             
Phe Gln Lys Leu Lys Glu Glu Ala Phe Glu Leu Ser Asp Gly Glu Ile 
    530                 535                 540                 
Thr Glu Leu Met Asn Ser Phe Ser Ser Lys Pro Glu Tyr Tyr Thr Pro 
545                 550                 555                 560 
Ile Val Thr Ser Ala Asp Val Ser Asn Asn Glu Ile Leu Ser Lys Ile 
                565                 570                 575     
Gln Gln Gln Phe Glu Lys Ile Leu Thr Lys Glu Asn Ser Ile Val Asn 
            580                 585                 590         
Gly Thr Ile Glu Asp Pro Met Gly Asp Lys Ile Asn Leu Gln Leu Gly 
        595                 600                 605             
Asn Gly Gln Thr Leu Gln Pro Ser Asp Tyr Thr Leu Gln Gly Asn Asp 
    610                 615                 620                 
Gly Ser Ile Met Lys Asp Ser Ile Ala Thr Gly Gly Pro Asn Asn Asp 
625                 630                 635                 640 
Gly Gly Ile Leu Lys Gly Val Lys Leu Glu Tyr Ile Lys Asn Lys Leu 
                645                 650                 655     
Tyr Val Arg Gly Leu Asn Leu Gly Glu Gly Gln Lys Val Thr Leu Thr 
            660                 665                 670         
Tyr Asp Val Lys Leu Asp Asp Ser Phe Ile Ser Asn Lys Phe Tyr Asp 
        675                 680                 685             
Thr Asn Gly Arg Thr Thr Leu Asn Pro Lys Ser Glu Glu Pro Asp Thr 
    690                 695                 700                 
Leu Arg Asp Phe Pro Ile Pro Lys Ile Arg Asp Val Arg Glu Tyr Pro 
705                 710                 715                 720 
Thr Ile Thr Ile Lys Asn Glu Lys Lys Leu Gly Glu Ile Glu Phe Thr 
                725                 730                 735     
Lys Val Asp Lys Asp Asn Asn Lys Leu Leu Leu Lys Gly Ala Thr Phe 
            740                 745                 750         
Glu Leu Gln Glu Phe Asn Glu Asp Tyr Lys Leu Tyr Leu Pro Ile Lys 
        755                 760                 765             
Asn Asn Asn Ser Lys Val Val Thr Gly Glu Asn Gly Lys Ile Ser Tyr 
    770                 775                 780                 
Lys Asp Leu Lys Asp Gly Lys Tyr Gln Leu Ile Glu Ala Val Ser Pro 
785                 790                 795                 800 
Lys Asp Tyr Gln Lys Ile Thr Asn Lys Pro Ile Leu Thr Phe Glu Val 
                805                 810                 815     
Val Lys Gly Ser Ile Gln Asn Ile Ile Ala Val Asn Lys Gln Ile Ser 
            820                 825                 830         
Glu Tyr His Glu Glu Gly Asp Lys His Leu Ile Thr Asn Thr His Ile 
        835                 840                 845             
Pro Pro Lys Gly Ile Ile Pro Met Thr Gly Gly Lys Gly Ile Leu Ser 
    850                 855                 860                 
Phe Ile Leu Ile Gly Gly Ala Met Met Ser Ile Ala Gly Gly Ile Tyr 
865                 870                 875                 880 
Ile Trp Lys Arg His Lys Lys Ser Ser Asp Ala Ser Ile Glu Lys Asp 
                885                 890                 895     



<210> 92
<211> 896
<212> PRT
<213> Streptococcus agalactiae CJB110

<220> 
<223> GBS67

<400> 92
Met Arg Lys Tyr Gln Lys Phe Ser Lys Ile Leu Thr Leu Ser Leu Phe 
1               5                   10                  15      
Cys Leu Ser Gln Ile Pro Leu Asn Thr Asn Val Leu Gly Glu Ser Thr 
            20                  25                  30          
Val Pro Glu Asn Gly Ala Lys Gly Lys Leu Val Val Lys Lys Thr Asp 
        35                  40                  45              
Asp Gln Asn Lys Pro Leu Ser Lys Ala Thr Phe Val Leu Lys Thr Thr 
    50                  55                  60                  
Ala His Pro Glu Ser Lys Ile Glu Lys Val Thr Ala Glu Val Thr Gly 
65                  70                  75                  80  
Glu Ala Thr Phe Asp Asn Leu Thr Pro Gly Asp Tyr Thr Leu Ser Glu 
                85                  90                  95      
Glu Thr Ala Pro Glu Gly Tyr Lys Lys Thr Thr Gln Thr Trp Gln Val 
            100                 105                 110         
Lys Val Glu Ser Asn Gly Lys Thr Thr Ile Gln Asn Ser Asp Asp Lys 
        115                 120                 125             
Lys Ser Ile Ile Glu Gln Arg Gln Glu Glu Leu Asp Lys Gln Tyr Pro 
    130                 135                 140                 
Leu Thr Gly Ala Tyr Glu Asp Thr Lys Glu Ser Tyr Asn Leu Glu His 
145                 150                 155                 160 
Val Lys Asn Ser Ile Pro Asn Gly Lys Leu Glu Ala Lys Ala Val Asn 
                165                 170                 175     
Pro Tyr Ser Ser Glu Gly Glu His Ile Arg Glu Ile Gln Glu Gly Thr 
            180                 185                 190         
Leu Ser Lys Arg Ile Ser Glu Val Asn Asp Leu Asp His Asn Lys Tyr 
        195                 200                 205             
Lys Ile Glu Leu Thr Val Ser Gly Lys Ser Ile Ile Lys Thr Ile Asn 
    210                 215                 220                 
Lys Asp Glu Pro Leu Asp Val Val Phe Val Leu Asp Asn Ser Asn Ser 
225                 230                 235                 240 
Met Lys Asn Asn Gly Lys Asn Asn Lys Ala Lys Lys Ala Gly Glu Ala 
                245                 250                 255     
Val Glu Thr Ile Ile Lys Asp Val Leu Gly Ala Asn Val Glu Asn Arg 
            260                 265                 270         
Ala Ala Leu Val Thr Tyr Gly Ser Asp Ile Phe Asp Gly Arg Thr Val 
        275                 280                 285             
Lys Val Ile Lys Gly Phe Lys Glu Asp Pro Tyr His Gly Leu Glu Thr 
    290                 295                 300                 
Ser Phe Thr Val Gln Thr Asn Asp Tyr Ser Tyr Lys Lys Phe Thr Asn 
305                 310                 315                 320 
Ile Ala Ala Asp Ile Ile Lys Lys Ile Pro Lys Glu Ala Pro Glu Ala 
                325                 330                 335     
Lys Trp Gly Gly Thr Ser Leu Gly Leu Thr Pro Glu Lys Lys Arg Glu 
            340                 345                 350         
Tyr Asp Leu Ser Lys Val Gly Glu Thr Phe Thr Met Lys Ala Phe Met 
        355                 360                 365             
Glu Ala Asp Thr Leu Leu Ser Ser Ile Gln Arg Lys Ser Arg Lys Ile 
    370                 375                 380                 
Ile Val His Leu Thr Asp Gly Val Pro Thr Arg Ser Tyr Ala Ile Asn 
385                 390                 395                 400 
Ser Phe Val Thr Gly Ser Thr Tyr Ala Asn Gln Phe Glu Arg Ile Lys 
                405                 410                 415     
Glu Lys Gly Tyr Leu Asp Lys Asn Asn Tyr Phe Ile Thr Asp Asp Pro 
            420                 425                 430         
Glu Lys Ile Lys Gly Asn Gly Glu Ser Tyr Phe Leu Phe Pro Leu Asp 
        435                 440                 445             
Ser Tyr Gln Thr Gln Ile Ile Ser Gly Asn Leu Gln Lys Leu His Tyr 
    450                 455                 460                 
Leu Asp Leu Asn Leu Asn Tyr Pro Lys Gly Thr Ile Tyr Arg Asn Gly 
465                 470                 475                 480 
Pro Val Arg Glu His Gly Thr Pro Thr Lys Leu Tyr Ile Asn Ser Leu 
                485                 490                 495     
Lys Gln Lys Asn Tyr Asp Ile Phe Asn Phe Gly Ile Asp Ile Ser Gly 
            500                 505                 510         
Phe Arg Gln Val Tyr Asn Glu Asp Tyr Lys Lys Asn Gln Asp Gly Thr 
        515                 520                 525             
Phe Gln Lys Leu Lys Glu Glu Ala Phe Glu Leu Ser Gly Gly Glu Ile 
    530                 535                 540                 
Thr Glu Leu Met Lys Ser Phe Ser Ser Lys Pro Glu Tyr Tyr Thr Pro 
545                 550                 555                 560 
Ile Val Thr Ser Ala Asp Val Ser Asn Asn Glu Ile Leu Ser Lys Ile 
                565                 570                 575     
Gln Gln Gln Phe Glu Lys Ile Leu Thr Lys Glu Asn Ser Ile Val Asn 
            580                 585                 590         
Gly Thr Ile Glu Asp Pro Met Gly Asp Lys Ile Asn Leu Gln Leu Gly 
        595                 600                 605             
Asn Gly Gln Thr Leu Gln Pro Ser Asp Tyr Thr Leu Gln Gly Asn Asp 
    610                 615                 620                 
Gly Ser Ile Met Lys Asp Ser Ile Ala Thr Gly Gly Pro Asn Asn Asp 
625                 630                 635                 640 
Gly Gly Ile Leu Lys Gly Val Lys Leu Glu Tyr Ile Lys Asn Lys Leu 
                645                 650                 655     
Tyr Val Arg Gly Leu Asn Leu Gly Glu Gly Gln Lys Val Thr Leu Thr 
            660                 665                 670         
Tyr Asp Val Lys Leu Asp Asp Ser Phe Ile Ser Asn Lys Phe Tyr Asp 
        675                 680                 685             
Thr Asn Gly Arg Thr Thr Leu Asn Pro Lys Ser Glu Glu Pro Asp Thr 
    690                 695                 700                 
Leu Arg Asp Phe Pro Ile Pro Lys Ile Arg Asp Val Arg Glu Tyr Pro 
705                 710                 715                 720 
Thr Ile Thr Ile Lys Asn Glu Lys Lys Leu Gly Glu Ile Glu Phe Thr 
                725                 730                 735     
Lys Val Asp Lys Asp Asn Asn Lys Leu Leu Leu Lys Gly Ala Thr Phe 
            740                 745                 750         
Glu Leu Gln Glu Phe Asn Glu Asp Tyr Lys Leu Tyr Leu Pro Ile Lys 
        755                 760                 765             
Asn Asn Asn Ser Lys Val Val Thr Gly Glu Asn Gly Lys Ile Ser Tyr 
    770                 775                 780                 
Lys Asp Leu Lys Asp Gly Lys Tyr Gln Leu Ile Glu Ala Val Ser Pro 
785                 790                 795                 800 
Lys Asp Tyr Gln Lys Ile Thr Asn Lys Pro Ile Leu Thr Phe Glu Val 
                805                 810                 815     
Val Lys Gly Ser Ile Gln Asn Ile Ile Ala Val Asn Lys Gln Ile Ser 
            820                 825                 830         
Glu Tyr His Glu Glu Gly Asp Lys His Leu Ile Thr Asn Thr His Ile 
        835                 840                 845             
Pro Pro Lys Gly Ile Ile Pro Met Thr Gly Gly Lys Gly Ile Leu Ser 
    850                 855                 860                 
Phe Ile Leu Ile Gly Gly Ala Met Met Ser Ile Ala Gly Gly Ile Tyr 
865                 870                 875                 880 
Ile Trp Lys Lys His Lys Lys Ser Ser Asp Ala Ser Ile Glu Lys Asp 
                885                 890                 895     



<210> 93
<211> 1434
<212> PRT
<213> Streptococcus agalactiae COH1

<220> 
<223> GBS1524/SAN1519

<400> 93
Met Leu Lys Lys Cys Gln Thr Phe Ile Ile Glu Ser Leu Lys Lys Lys 
1               5                   10                  15      
Lys His Pro Lys Glu Trp Lys Ile Ile Met Trp Ser Leu Met Ile Leu 
            20                  25                  30          
Thr Thr Phe Leu Thr Thr Tyr Phe Leu Ile Leu Pro Ala Ile Thr Val 
        35                  40                  45              
Glu Glu Thr Lys Thr Asp Asp Val Gly Ile Thr Leu Glu Asn Lys Asn 
    50                  55                  60                  
Ser Ser Gln Val Thr Ser Ser Thr Ser Ser Ser Gln Ser Ser Val Glu 
65                  70                  75                  80  
Gln Ser Lys Pro Gln Thr Pro Ala Ser Ser Val Thr Glu Thr Ser Ser 
                85                  90                  95      
Ser Glu Glu Ala Ala Tyr Arg Glu Glu Pro Leu Met Phe Arg Gly Ala 
            100                 105                 110         
Asp Tyr Thr Val Thr Val Thr Leu Thr Lys Glu Ala Lys Ile Pro Lys 
        115                 120                 125             
Asn Ala Asp Leu Lys Val Thr Glu Leu Lys Asp Asn Ser Ala Thr Phe 
    130                 135                 140                 
Lys Asp Tyr Lys Lys Lys Ala Leu Thr Glu Val Ala Lys Gln Asp Ser 
145                 150                 155                 160 
Glu Ile Lys Asn Phe Lys Leu Tyr Asp Ile Thr Ile Glu Ser Asn Gly 
                165                 170                 175     
Lys Glu Ala Glu Pro Gln Ala Pro Val Lys Val Glu Val Asn Tyr Asp 
            180                 185                 190         
Lys Pro Leu Glu Ala Ser Asp Glu Asn Leu Lys Val Val His Phe Lys 
        195                 200                 205             
Asp Asp Gly Gln Thr Glu Val Leu Lys Ser Lys Asp Thr Ala Glu Thr 
    210                 215                 220                 
Lys Asn Thr Ser Ser Asp Val Ala Phe Lys Thr Asp Ser Phe Ser Ile 
225                 230                 235                 240 
Tyr Ala Ile Val Gln Glu Asp Asn Thr Glu Val Pro Arg Leu Thr Tyr 
                245                 250                 255     
His Phe Gln Asn Asn Asp Gly Thr Asp Tyr Asp Phe Leu Thr Ala Ser 
            260                 265                 270         
Gly Met Gln Val His His Gln Ile Ile Lys Asp Gly Glu Ser Leu Gly 
        275                 280                 285             
Glu Val Gly Ile Pro Thr Ile Lys Ala Gly Glu His Phe Asn Gly Trp 
    290                 295                 300                 
Tyr Thr Tyr Asp Pro Thr Thr Gly Lys Tyr Gly Asp Pro Val Lys Phe 
305                 310                 315                 320 
Gly Glu Pro Ile Thr Val Thr Glu Thr Lys Glu Ile Cys Val Arg Pro 
                325                 330                 335     
Phe Met Ser Lys Val Ala Thr Val Thr Leu Tyr Asp Asp Ser Ala Gly 
            340                 345                 350         
Lys Ser Ile Leu Glu Arg Tyr Gln Val Pro Leu Asp Ser Ser Gly Asn 
        355                 360                 365             
Gly Thr Ala Asp Leu Ser Ser Phe Lys Val Ser Pro Pro Thr Ser Thr 
    370                 375                 380                 
Leu Leu Phe Val Gly Trp Ser Lys Thr Gln Asn Gly Ala Pro Leu Ser 
385                 390                 395                 400 
Glu Ser Glu Ile Gln Ala Leu Pro Val Ser Ser Asp Ile Ser Leu Tyr 
                405                 410                 415     
Pro Val Phe Lys Glu Ser Tyr Gly Val Glu Phe Asn Thr Gly Asp Leu 
            420                 425                 430         
Ser Thr Gly Val Thr Tyr Ile Ala Pro Arg Arg Val Leu Thr Gly Gln 
        435                 440                 445             
Pro Ala Ser Thr Ile Lys Pro Asn Asp Pro Thr Arg Pro Gly Tyr Thr 
    450                 455                 460                 
Phe Ala Gly Trp Tyr Thr Ala Ala Ser Gly Gly Ala Ala Phe Asp Phe 
465                 470                 475                 480 
Asn Gln Val Leu Thr Lys Asp Thr Thr Leu Tyr Ala His Trp Ser Pro 
                485                 490                 495     
Ala Gln Thr Thr Tyr Thr Ile Asn Tyr Trp Gln Gln Ser Ala Thr Asp 
            500                 505                 510         
Asn Lys Asn Ala Thr Asp Ala Gln Lys Thr Tyr Glu Tyr Ala Gly Gln 
        515                 520                 525             
Val Thr Arg Ser Gly Leu Ser Leu Ser Asn Gln Thr Leu Thr Gln Gln 
    530                 535                 540                 
Asp Ile Asn Asp Lys Leu Pro Thr Gly Phe Lys Val Asn Asn Thr Arg 
545                 550                 555                 560 
Thr Glu Thr Ser Val Met Ile Lys Asp Asp Gly Ser Ser Val Val Asn 
                565                 570                 575     
Val Tyr Tyr Asp Arg Lys Leu Ile Thr Ile Lys Phe Ala Lys Tyr Gly 
            580                 585                 590         
Gly Tyr Ser Leu Pro Glu Tyr Tyr Tyr Ser Tyr Asn Trp Ser Ser Asp 
        595                 600                 605             
Ala Asp Thr Tyr Thr Gly Leu Tyr Gly Thr Thr Leu Ala Ala Asn Gly 
    610                 615                 620                 
Tyr Gln Trp Lys Thr Gly Ala Trp Gly Tyr Leu Ala Asn Val Gly Asn 
625                 630                 635                 640 
Asn Gln Val Gly Thr Tyr Gly Met Ser Tyr Leu Gly Glu Phe Ile Leu 
                645                 650                 655     
Pro Asn Asp Thr Val Asp Ser Asp Val Ile Lys Leu Phe Pro Lys Gly 
            660                 665                 670         
Asn Ile Val Gln Thr Tyr Arg Phe Phe Lys Gln Gly Leu Asp Gly Thr 
        675                 680                 685             
Tyr Ser Leu Ala Asp Thr Gly Gly Gly Ala Gly Ala Asp Glu Phe Thr 
    690                 695                 700                 
Phe Thr Glu Lys Tyr Leu Gly Phe Asn Val Lys Tyr Tyr Gln Arg Leu 
705                 710                 715                 720 
Tyr Pro Asp Asn Tyr Leu Phe Asp Gln Tyr Ala Ser Gln Thr Ser Ala 
                725                 730                 735     
Gly Val Lys Val Pro Ile Ser Asp Glu Tyr Tyr Asp Arg Tyr Gly Ala 
            740                 745                 750         
Tyr His Lys Asp Tyr Leu Asn Leu Val Val Trp Tyr Glu Arg Asn Ser 
        755                 760                 765             
Tyr Lys Ile Lys Tyr Leu Asp Pro Leu Asp Asn Thr Glu Leu Pro Asn 
    770                 775                 780                 
Phe Pro Val Lys Asp Val Leu Tyr Glu Gln Asn Leu Ser Ser Tyr Ala 
785                 790                 795                 800 
Pro Asp Thr Thr Thr Val Gln Pro Lys Pro Ser Arg Pro Gly Tyr Val 
                805                 810                 815     
Trp Asp Gly Lys Trp Tyr Lys Asp Gln Ala Gln Thr Gln Val Phe Asp 
            820                 825                 830         
Phe Asn Thr Thr Met Pro Pro His Asp Val Lys Val Tyr Ala Gly Trp 
        835                 840                 845             
Gln Lys Val Thr Tyr Arg Val Asn Ile Asp Pro Asn Gly Gly Arg Leu 
    850                 855                 860                 
Ser Lys Thr Asp Asp Thr Tyr Leu Asp Leu His Tyr Gly Asp Arg Ile 
865                 870                 875                 880 
Pro Asp Tyr Thr Asp Ile Thr Arg Asp Tyr Ile Gln Asp Pro Ser Gly 
                885                 890                 895     
Thr Tyr Tyr Tyr Lys Tyr Asp Ser Arg Asp Lys Asp Pro Asp Ser Thr 
            900                 905                 910         
Lys Asp Ala Tyr Tyr Thr Thr Asp Thr Ser Leu Ser Asn Val Asp Thr 
        915                 920                 925             
Thr Thr Lys Tyr Lys Tyr Val Lys Asp Ala Tyr Lys Leu Val Gly Trp 
    930                 935                 940                 
Tyr Tyr Val Asn Pro Asp Gly Ser Ile Arg Pro Tyr Asn Phe Ser Gly 
945                 950                 955                 960 
Ala Val Thr Gln Asp Ile Asn Leu Arg Ala Ile Trp Arg Lys Ala Gly 
                965                 970                 975     
Asp Tyr His Ile Ile Tyr Ser Asn Asp Ala Val Gly Thr Asp Gly Lys 
            980                 985                 990         
Pro Ala Leu Asp Ala Ser Gly Gln Gln Leu Gln Thr Ser Asn Glu Pro 
        995                 1000                1005            
Thr Asp Pro Asp Ser Tyr Asp Asp Gly Ser His Ser Ala Leu Leu Arg 
    1010                1015                1020                
Arg Pro Thr Met Pro Asp Gly Tyr Arg Phe Arg Gly Trp Trp Tyr Asn 
1025                1030                1035                1040
Gly Lys Ile Tyr Asn Pro Tyr Asp Ser Ile Asp Ile Asp Ala His Leu 
                1045                1050                1055    
Ala Asp Ala Asn Lys Asn Ile Thr Ile Lys Pro Val Ile Ile Pro Val 
            1060                1065                1070        
Gly Asp Ile Lys Leu Glu Asp Thr Ser Ile Lys Tyr Asn Gly Asn Gly 
        1075                1080                1085            
Gly Thr Arg Val Glu Asn Gly Asn Val Val Thr Gln Val Glu Thr Pro 
    1090                1095                1100                
Arg Met Glu Leu Asn Ser Thr Thr Thr Ile Pro Glu Asn Gln Tyr Phe 
1105                1110                1115                1120
Thr Arg Thr Gly Tyr Asn Leu Ile Gly Trp His His Asp Lys Asp Leu 
                1125                1130                1135    
Ala Asp Thr Gly Arg Val Glu Phe Thr Ala Gly Gln Ser Ile Gly Ile 
            1140                1145                1150        
Asp Asn Asn Pro Asp Ala Thr Asn Thr Leu Tyr Ala Val Trp Gln Pro 
        1155                1160                1165            
Lys Glu Tyr Thr Val Arg Val Ser Lys Thr Val Val Gly Leu Asp Glu 
    1170                1175                1180                
Asp Lys Thr Lys Asp Phe Leu Phe Asn Pro Ser Glu Thr Leu Gln Gln 
1185                1190                1195                1200
Glu Asn Phe Pro Leu Arg Asp Gly Gln Thr Lys Glu Phe Lys Val Pro 
                1205                1210                1215    
Tyr Gly Thr Ser Ile Ser Ile Asp Glu Gln Ala Tyr Asp Glu Phe Lys 
            1220                1225                1230        
Val Ser Glu Ser Ile Thr Glu Lys Asn Leu Ala Thr Gly Glu Ala Asp 
        1235                1240                1245            
Lys Thr Tyr Asp Ala Thr Gly Leu Gln Ser Leu Thr Val Ser Gly Asp 
    1250                1255                1260                
Val Asp Ile Ser Phe Thr Asn Thr Arg Ile Lys Gln Lys Val Arg Leu 
1265                1270                1275                1280
Gln Lys Val Asn Val Glu Asn Asp Asn Asn Phe Leu Ala Gly Ala Val 
                1285                1290                1295    
Phe Asp Ile Tyr Glu Ser Asp Ala Asn Gly Asn Lys Ala Ser His Pro 
            1300                1305                1310        
Met Tyr Ser Gly Leu Val Thr Asn Asp Lys Gly Leu Leu Leu Val Asp 
        1315                1320                1325            
Ala Asn Asn Tyr Leu Ser Leu Pro Val Gly Lys Tyr Tyr Leu Thr Glu 
    1330                1335                1340                
Thr Lys Ala Pro Pro Gly Tyr Leu Leu Pro Lys Asn Asp Ile Ser Val 
1345                1350                1355                1360
Leu Val Ile Ser Thr Gly Val Thr Phe Glu Gln Asn Gly Asn Asn Ala 
                1365                1370                1375    
Thr Pro Ile Lys Glu Asn Leu Val Asp Gly Ser Thr Val Tyr Thr Phe 
            1380                1385                1390        
Lys Ile Thr Asn Ser Lys Gly Thr Glu Leu Pro Ser Thr Gly Gly Ile 
        1395                1400                1405            
Gly Thr His Ile Tyr Ile Leu Val Gly Leu Ala Leu Ala Leu Pro Ser 
    1410                1415                1420                
Gly Leu Ile Leu Tyr Tyr Arg Lys Lys Ile 
1425                1430                

<210> 94
<211> 886
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> RrgA

<400> 94
Met Lys Lys Val Arg Lys Ile Phe Gln Lys Ala Val Ala Gly Leu Cys 
1               5                   10                  15      
Cys Ile Ser Gln Leu Thr Ala Phe Ser Ser Ile Val Ala Leu Ala Glu 
            20                  25                  30          
Thr Pro Glu Thr Ser Pro Ala Ile Gly Lys Val Val Ile Lys Glu Thr 
        35                  40                  45              
Gly Glu Gly Gly Ala Leu Leu Gly Asp Ala Val Phe Glu Leu Lys Asn 
    50                  55                  60                  
Asn Thr Asp Gly Thr Thr Val Ser Gln Arg Thr Glu Ala Gln Thr Gly 
65                  70                  75                  80  
Glu Ala Ile Phe Ser Asn Ile Lys Pro Gly Thr Tyr Thr Leu Thr Glu 
                85                  90                  95      
Ala Gln Pro Pro Val Gly Tyr Lys Pro Ser Thr Lys Gln Trp Thr Val 
            100                 105                 110         
Glu Val Glu Lys Asn Gly Arg Thr Thr Val Gln Gly Glu Gln Val Glu 
        115                 120                 125             
Asn Arg Glu Glu Ala Leu Ser Asp Gln Tyr Pro Gln Thr Gly Thr Tyr 
    130                 135                 140                 
Pro Asp Val Gln Thr Pro Tyr Gln Ile Ile Lys Val Asp Gly Ser Glu 
145                 150                 155                 160 
Lys Asn Gly Gln His Lys Ala Leu Asn Pro Asn Pro Tyr Glu Arg Val 
                165                 170                 175     
Ile Pro Glu Gly Thr Leu Ser Lys Arg Ile Tyr Gln Val Asn Asn Leu 
            180                 185                 190         
Asp Asp Asn Gln Tyr Gly Ile Glu Leu Thr Val Ser Gly Lys Thr Val 
        195                 200                 205             
Tyr Glu Gln Lys Asp Lys Ser Val Pro Leu Asp Val Val Ile Leu Leu 
    210                 215                 220                 
Asp Asn Ser Asn Ser Met Ser Asn Ile Arg Asn Lys Asn Ala Arg Arg 
225                 230                 235                 240 
Ala Glu Arg Ala Gly Glu Ala Thr Arg Ser Leu Ile Asp Lys Ile Thr 
                245                 250                 255     
Ser Asp Pro Glu Asn Arg Val Ala Leu Val Thr Tyr Ala Ser Thr Ile 
            260                 265                 270         
Phe Asp Gly Thr Glu Phe Thr Val Glu Lys Gly Val Ala Asp Lys Asn 
        275                 280                 285             
Gly Lys Arg Leu Asn Asp Ser Leu Phe Trp Asn Tyr Asp Gln Thr Ser 
    290                 295                 300                 
Phe Thr Thr Asn Thr Lys Asp Tyr Ser Tyr Leu Lys Leu Thr Asn Asp 
305                 310                 315                 320 
Lys Asn Asp Ile Val Glu Leu Lys Asn Lys Val Pro Thr Glu Ala Glu 
                325                 330                 335     
Asp His Asp Gly Asn Arg Leu Met Tyr Gln Phe Gly Ala Thr Phe Thr 
            340                 345                 350         
Gln Lys Ala Leu Met Lys Ala Asp Glu Ile Leu Thr Gln Gln Ala Arg 
        355                 360                 365             
Gln Asn Ser Gln Lys Val Ile Phe His Ile Thr Asp Gly Val Pro Thr 
    370                 375                 380                 
Met Ser Tyr Pro Ile Asn Phe Asn His Ala Thr Phe Ala Pro Ser Tyr 
385                 390                 395                 400 
Gln Asn Gln Leu Asn Ala Phe Phe Ser Lys Ser Pro Asn Lys Asp Gly 
                405                 410                 415     
Ile Leu Leu Ser Asp Phe Ile Thr Gln Ala Thr Ser Gly Glu His Thr 
            420                 425                 430         
Ile Val Arg Gly Asp Gly Gln Ser Tyr Gln Met Phe Thr Asp Lys Thr 
        435                 440                 445             
Val Tyr Glu Lys Gly Ala Pro Ala Ala Phe Pro Val Lys Pro Glu Lys 
    450                 455                 460                 
Tyr Ser Glu Met Lys Ala Ala Gly Tyr Ala Val Ile Gly Asp Pro Ile 
465                 470                 475                 480 
Asn Gly Gly Tyr Ile Trp Leu Asn Trp Arg Glu Ser Ile Leu Ala Tyr 
                485                 490                 495     
Pro Phe Asn Ser Asn Thr Ala Lys Ile Thr Asn His Gly Asp Pro Thr 
            500                 505                 510         
Arg Trp Tyr Tyr Asn Gly Asn Ile Ala Pro Asp Gly Tyr Asp Val Phe 
        515                 520                 525             
Thr Val Gly Ile Gly Ile Asn Gly Asp Pro Gly Thr Asp Glu Ala Thr 
    530                 535                 540                 
Ala Thr Ser Phe Met Gln Ser Ile Ser Ser Lys Pro Glu Asn Tyr Thr 
545                 550                 555                 560 
Asn Val Thr Asp Thr Thr Lys Ile Leu Glu Gln Leu Asn Arg Tyr Phe 
                565                 570                 575     
His Thr Ile Val Thr Glu Lys Lys Ser Ile Glu Asn Gly Thr Ile Thr 
            580                 585                 590         
Asp Pro Met Gly Glu Leu Ile Asp Leu Gln Leu Gly Thr Asp Gly Arg 
        595                 600                 605             
Phe Asp Pro Ala Asp Tyr Thr Leu Thr Ala Asn Asp Gly Ser Arg Leu 
    610                 615                 620                 
Glu Asn Gly Gln Ala Val Gly Gly Pro Gln Asn Asp Gly Gly Leu Leu 
625                 630                 635                 640 
Lys Asn Ala Lys Val Leu Tyr Asp Thr Thr Glu Lys Arg Ile Arg Val 
                645                 650                 655     
Thr Gly Leu Tyr Leu Gly Thr Asp Glu Lys Val Thr Leu Thr Tyr Asn 
            660                 665                 670         
Val Arg Leu Asn Asp Glu Phe Val Ser Asn Lys Phe Tyr Asp Thr Asn 
        675                 680                 685             
Gly Arg Thr Thr Leu His Pro Lys Glu Val Glu Gln Asn Thr Val Arg 
    690                 695                 700                 
Asp Phe Pro Ile Pro Lys Ile Arg Asp Val Arg Lys Tyr Pro Glu Ile 
705                 710                 715                 720 
Thr Ile Ser Lys Glu Lys Lys Leu Gly Asp Ile Glu Phe Ile Lys Val 
                725                 730                 735     
Asn Lys Asn Asp Lys Lys Pro Leu Arg Asp Ala Val Phe Ser Leu Gln 
            740                 745                 750         
Lys Gln His Pro Asp Tyr Pro Asp Ile Tyr Gly Ala Ile Asp Gln Asn 
        755                 760                 765             
Gly Thr Tyr Gln Asn Val Arg Thr Gly Glu Asp Gly Lys Leu Thr Phe 
    770                 775                 780                 
Lys Asn Leu Ser Asp Gly Lys Tyr Arg Leu Phe Glu Asn Ser Glu Pro 
785                 790                 795                 800 
Ala Gly Tyr Lys Pro Val Gln Asn Lys Pro Ile Val Ala Phe Gln Ile 
                805                 810                 815     
Val Asn Gly Glu Val Arg Asp Val Thr Ser Ile Val Pro Gln Asp Ile 
            820                 825                 830         
Pro Ala Gly Tyr Glu Phe Thr Asn Asp Lys His Tyr Ile Thr Asn Glu 
        835                 840                 845             
Pro Ile Pro Pro Lys Arg Glu Tyr Pro Arg Thr Gly Gly Ile Gly Met 
    850                 855                 860                 
Leu Pro Phe Tyr Leu Ile Gly Cys Met Met Met Gly Gly Val Leu Leu 
865                 870                 875                 880 
Tyr Thr Arg Lys His Pro 
                885     

<210> 95
<211> 307
<212> PRT
<213> Streptococcus agalactiae 2603V/R

<220> 
<223> GBS052/SAG0646

<400> 95
Met Lys Gln Thr Leu Lys Leu Met Phe Ser Phe Leu Leu Met Leu Gly 
1               5                   10                  15      
Thr Met Phe Gly Ile Ser Gln Thr Val Leu Ala Gln Glu Thr His Gln 
            20                  25                  30          
Leu Thr Ile Val His Leu Glu Ala Arg Asp Ile Asp Arg Pro Asn Pro 
        35                  40                  45              
Gln Leu Glu Ile Ala Pro Lys Glu Gly Thr Pro Ile Glu Gly Val Leu 
    50                  55                  60                  
Tyr Gln Leu Tyr Gln Leu Lys Ser Thr Glu Asp Gly Asp Leu Leu Ala 
65                  70                  75                  80  
His Trp Asn Ser Leu Thr Ile Thr Glu Leu Lys Lys Gln Ala Gln Gln 
                85                  90                  95      
Val Phe Glu Ala Thr Thr Asn Gln Gln Gly Lys Ala Thr Phe Asn Gln 
            100                 105                 110         
Leu Pro Asp Gly Ile Tyr Tyr Gly Leu Ala Val Lys Ala Gly Glu Lys 
        115                 120                 125             
Asn Arg Asn Val Ser Ala Phe Leu Val Asp Leu Ser Glu Asp Lys Val 
    130                 135                 140                 
Ile Tyr Pro Lys Ile Ile Trp Ser Thr Gly Glu Leu Asp Leu Leu Lys 
145                 150                 155                 160 
Val Gly Val Asp Gly Asp Thr Lys Lys Pro Leu Ala Gly Val Val Phe 
                165                 170                 175     
Glu Leu Tyr Glu Lys Asn Gly Arg Thr Pro Ile Arg Val Lys Asn Gly 
            180                 185                 190         
Val His Ser Gln Asp Ile Asp Ala Ala Lys His Leu Glu Thr Asp Ser 
        195                 200                 205             
Ser Gly His Ile Arg Ile Ser Gly Leu Ile His Gly Asp Tyr Val Leu 
    210                 215                 220                 
Lys Glu Ile Glu Thr Gln Ser Gly Tyr Gln Ile Gly Gln Ala Glu Thr 
225                 230                 235                 240 
Ala Val Thr Ile Glu Lys Ser Lys Thr Val Thr Val Thr Ile Glu Asn 
                245                 250                 255     
Lys Lys Val Pro Thr Pro Lys Val Pro Ser Arg Gly Gly Leu Ile Pro 
            260                 265                 270         
Lys Thr Gly Glu Gln Gln Ala Met Ala Leu Val Ile Ile Gly Gly Ile 
        275                 280                 285             
Leu Ile Ala Leu Ala Leu Arg Leu Leu Ser Lys His Arg Lys His Gln 
    290                 295                 300                 
Asn Lys Asp 
305         

<210> 96
<211> 308
<212> PRT
<213> Streptococcus agalactiae 2603V/R

<220> 
<223> GBS150/SAG1404

<400> 96
Met Lys Lys Ile Arg Lys Ser Leu Gly Leu Leu Leu Cys Cys Phe Leu 
1               5                   10                  15      
Gly Leu Val Gln Leu Ala Phe Phe Ser Val Ala Ser Val Asn Ala Asp 
            20                  25                  30          
Thr Pro Asn Gln Leu Thr Ile Thr Gln Ile Gly Leu Gln Pro Asn Thr 
        35                  40                  45              
Thr Glu Glu Gly Ile Ser Tyr Arg Leu Trp Thr Val Thr Asp Asn Leu 
    50                  55                  60                  
Lys Val Asp Leu Leu Ser Gln Met Thr Asp Ser Glu Leu Asn Gln Lys 
65                  70                  75                  80  
Tyr Lys Ser Ile Leu Thr Ser Pro Thr Asp Thr Asn Gly Gln Thr Lys 
                85                  90                  95      
Ile Ala Leu Pro Asn Gly Ser Tyr Phe Gly Arg Ala Tyr Lys Ala Asp 
            100                 105                 110         
Gln Ser Val Ser Thr Ile Val Pro Phe Tyr Ile Glu Leu Pro Asp Asp 
        115                 120                 125             
Lys Leu Ser Asn Gln Leu Gln Ile Asn Pro Lys Arg Lys Val Glu Thr 
    130                 135                 140                 
Gly Arg Leu Lys Leu Ile Lys Tyr Thr Lys Glu Gly Lys Ile Lys Lys 
145                 150                 155                 160 
Arg Leu Ser Gly Val Ile Phe Val Leu Tyr Asp Asn Gln Asn Gln Pro 
                165                 170                 175     
Val Arg Phe Lys Asn Gly Arg Phe Thr Thr Asp Gln Asp Gly Ile Thr 
            180                 185                 190         
Ser Leu Val Thr Asp Asp Lys Gly Glu Ile Glu Val Glu Gly Leu Leu 
        195                 200                 205             
Pro Gly Lys Tyr Ile Phe Arg Glu Ala Lys Ala Leu Thr Gly Tyr Arg 
    210                 215                 220                 
Ile Ser Met Lys Asp Ala Val Val Ala Val Val Ala Asn Lys Thr Gln 
225                 230                 235                 240 
Glu Val Glu Val Glu Asn Glu Lys Glu Thr Pro Pro Pro Thr Asn Pro 
                245                 250                 255     
Lys Pro Ser Gln Pro Leu Phe Pro Gln Ser Phe Leu Pro Lys Thr Gly 
            260                 265                 270         
Met Ile Ile Gly Gly Gly Leu Thr Ile Leu Gly Cys Ile Ile Leu Gly 
        275                 280                 285             
Ile Leu Phe Ile Phe Leu Arg Lys Thr Lys Asn Ser Lys Ser Glu Arg 
    290                 295                 300                 
Asn Asp Thr Val 
305             

<210> 97
<211> 401
<212> PRT
<213> Streptococcus pneumoniae

<220> 
<223> RrgC

<400> 97
Met Thr Met Gln Lys Met Gln Lys Met Ile Ser Arg Ile Phe Phe Val 
1               5                   10                  15      
Met Ala Leu Cys Phe Ser Leu Val Trp Gly Ala His Ala Val Gln Ala 
            20                  25                  30          
Gln Glu Asp His Thr Leu Val Leu Gln Leu Glu Asn Tyr Gln Glu Val 
        35                  40                  45              
Val Ser Gln Leu Pro Ser Arg Asp Gly His Arg Leu Gln Val Trp Lys 
    50                  55                  60                  
Leu Asp Asp Ser Tyr Ser Tyr Asp Asp Arg Val Gln Ile Val Arg Asp 
65                  70                  75                  80  
Leu His Ser Trp Asp Glu Asn Lys Leu Ser Ser Phe Lys Lys Thr Ser 
                85                  90                  95      
Phe Glu Met Thr Phe Leu Glu Asn Gln Ile Glu Val Ser His Ile Pro 
            100                 105                 110         
Asn Gly Leu Tyr Tyr Val Arg Ser Ile Ile Gln Thr Asp Ala Val Ser 
        115                 120                 125             
Tyr Pro Ala Glu Phe Leu Phe Glu Met Thr Asp Gln Thr Val Glu Pro 
    130                 135                 140                 
Leu Val Ile Val Ala Lys Lys Thr Asp Thr Met Thr Thr Lys Val Lys 
145                 150                 155                 160 
Leu Ile Lys Val Asp Gln Asp His Asn Arg Leu Glu Gly Val Gly Phe 
                165                 170                 175     
Lys Leu Val Ser Val Ala Arg Asp Gly Ser Glu Lys Glu Val Pro Leu 
            180                 185                 190         
Ile Gly Glu Tyr Arg Tyr Ser Ser Ser Gly Gln Val Gly Arg Thr Leu 
        195                 200                 205             
Tyr Thr Asp Lys Asn Gly Glu Ile Phe Val Thr Asn Leu Pro Leu Gly 
    210                 215                 220                 
Asn Tyr Arg Phe Lys Glu Val Glu Pro Leu Ala Gly Tyr Ala Val Thr 
225                 230                 235                 240 
Thr Leu Asp Thr Asp Val Gln Leu Val Asp His Gln Leu Val Thr Ile 
                245                 250                 255     
Thr Val Val Asn Gln Lys Leu Pro Arg Gly Asn Val Asp Phe Met Lys 
            260                 265                 270         
Val Asp Gly Arg Thr Asn Thr Ser Leu Gln Gly Ala Met Phe Lys Val 
        275                 280                 285             
Met Lys Glu Glu Ser Gly His Tyr Thr Pro Val Leu Gln Asn Gly Lys 
    290                 295                 300                 
Glu Val Val Val Thr Ser Gly Lys Asp Gly Arg Phe Arg Val Glu Gly 
305                 310                 315                 320 
Leu Glu Tyr Gly Thr Tyr Tyr Leu Trp Glu Leu Gln Ala Pro Thr Gly 
                325                 330                 335     
Tyr Val Gln Leu Thr Ser Pro Val Ser Phe Thr Ile Gly Lys Asp Thr 
            340                 345                 350         
Arg Lys Glu Leu Val Thr Val Val Lys Asn Asn Lys Arg Pro Arg Ile 
        355                 360                 365             
Asp Val Pro Asp Thr Gly Glu Glu Thr Leu Tyr Ile Leu Met Leu Val 
    370                 375                 380                 
Ala Ile Leu Leu Phe Gly Ser Gly Tyr Tyr Leu Thr Lys Lys Pro Asn 
385                 390                 395                 400 
Asn 
    

<210> 98
<211> 255
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> SrtC1 lacking N-terminal transmembrane domain

<400> 98
His Gln Ser Arg Ala Ile Met Asp Tyr Gln Asp Arg Val Thr His Met 
1               5                   10                  15      
Asp Glu Asn Asp Tyr Lys Lys Ile Ile Asn Arg Ala Lys Glu Tyr Asn 
            20                  25                  30          
Lys Gln Phe Lys Thr Ser Gly Met Lys Trp His Met Thr Ser Gln Glu 
        35                  40                  45              
Arg Leu Asp Tyr Asn Ser Gln Leu Ala Ile Asp Lys Thr Gly Asn Met 
    50                  55                  60                  
Gly Tyr Ile Ser Ile Pro Lys Ile Asn Ile Lys Leu Pro Leu Tyr His 
65                  70                  75                  80  
Gly Thr Ser Glu Lys Val Leu Gln Thr Ser Ile Gly His Leu Glu Gly 
                85                  90                  95      
Ser Ser Leu Pro Ile Gly Gly Asp Ser Thr His Ser Ile Leu Ser Gly 
            100                 105                 110         
His Arg Gly Leu Pro Ser Ser Arg Leu Phe Ser Asp Leu Asp Lys Leu 
        115                 120                 125             
Lys Val Gly Asp His Trp Thr Val Ser Ile Leu Asn Glu Thr Tyr Thr 
    130                 135                 140                 
Tyr Gln Val Asp Gln Ile Arg Thr Val Lys Pro Asp Asp Leu Arg Asp 
145                 150                 155                 160 
Leu Gln Ile Val Lys Gly Lys Asp Tyr Gln Thr Leu Val Thr Cys Thr 
                165                 170                 175     
Pro Tyr Gly Val Asn Thr His Arg Leu Leu Val Arg Gly His Arg Val 
            180                 185                 190         
Pro Asn Asp Asn Gly Asn Ala Leu Val Val Ala Glu Ala Ile Gln Ile 
        195                 200                 205             
Glu Pro Ile Tyr Ile Ala Pro Phe Ile Ala Ile Phe Leu Thr Leu Ile 
    210                 215                 220                 
Leu Leu Leu Ile Ser Leu Glu Val Thr Arg Arg Ala Arg Gln Arg Lys 
225                 230                 235                 240 
Lys Ile Leu Lys Gln Ala Met Arg Lys Glu Glu Asn Asn Asp Leu 
                245                 250                 255 

<210> 99
<211> 203
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> SrtC1

<400> 99
Asn Ser Gln Leu Ala Ile Asp Lys Thr Gly Asn Met Gly Tyr Ile Ser 
1               5                   10                  15      
Ile Pro Lys Ile Asn Ile Lys Leu Pro Leu Tyr His Gly Thr Ser Glu 
            20                  25                  30          
Lys Val Leu Gln Thr Ser Ile Gly His Leu Glu Gly Ser Ser Leu Pro 
        35                  40                  45              
Ile Gly Gly Asp Ser Thr His Ser Ile Leu Ser Gly His Arg Gly Leu 
    50                  55                  60                  
Pro Ser Ser Arg Leu Phe Ser Asp Leu Asp Lys Leu Lys Val Gly Asp 
65                  70                  75                  80  
His Trp Thr Val Ser Ile Leu Asn Glu Thr Tyr Thr Tyr Gln Val Asp 
                85                  90                  95      
Gln Ile Arg Thr Val Lys Pro Asp Asp Leu Arg Asp Leu Gln Ile Val 
            100                 105                 110         
Lys Gly Lys Asp Tyr Gln Thr Leu Val Thr Cys Thr Pro Tyr Gly Val 
        115                 120                 125             
Asn Thr His Arg Leu Leu Val Arg Gly His Arg Val Pro Asn Asp Asn 
    130                 135                 140                 
Gly Asn Ala Leu Val Val Ala Glu Ala Ile Gln Ile Glu Pro Ile Tyr 
145                 150                 155                 160 
Ile Ala Pro Phe Ile Ala Ile Phe Leu Thr Leu Ile Leu Leu Leu Ile 
                165                 170                 175     
Ser Leu Glu Val Thr Arg Arg Ala Arg Gln Arg Lys Lys Ile Leu Lys 
            180                 185                 190         
Gln Ala Met Arg Lys Glu Glu Asn Asn Asp Leu 
        195                 200             

<210> 100
<211> 254
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> srtC1

<400> 100
Gln Ser Arg Ala Ile Met Asp Tyr Gln Asp Arg Val Thr His Met Asp 
1               5                   10                  15      
Glu Asn Asp Tyr Lys Lys Ile Ile Asn Arg Ala Lys Glu Tyr Asn Lys 
            20                  25                  30          
Gln Phe Lys Thr Ser Gly Met Lys Trp His Met Thr Ser Gln Glu Arg 
        35                  40                  45              
Leu Asp Tyr Asn Ser Gln Leu Ala Ile Asp Lys Thr Gly Asn Met Gly 
    50                  55                  60                  
Tyr Ile Ser Ile Pro Lys Ile Asn Ile Lys Leu Pro Leu Tyr His Gly 
65                  70                  75                  80  
Thr Ser Glu Lys Val Leu Gln Thr Ser Ile Gly His Leu Glu Gly Ser 
                85                  90                  95      
Ser Leu Pro Ile Gly Gly Asp Ser Thr His Ser Ile Leu Ser Gly His 
            100                 105                 110         
Arg Gly Leu Pro Ser Ser Arg Leu Phe Ser Asp Leu Asp Lys Leu Lys 
        115                 120                 125             
Val Gly Asp His Trp Thr Val Ser Ile Leu Asn Glu Thr Tyr Thr Tyr 
    130                 135                 140                 
Gln Val Asp Gln Ile Arg Thr Val Lys Pro Asp Asp Leu Arg Asp Leu 
145                 150                 155                 160 
Gln Ile Val Lys Gly Lys Asp Tyr Gln Thr Leu Val Thr Cys Thr Pro 
                165                 170                 175     
Tyr Gly Val Asn Thr His Arg Leu Leu Val Arg Gly His Arg Val Pro 
            180                 185                 190         
Asn Asp Asn Gly Asn Ala Leu Val Val Ala Glu Ala Ile Gln Ile Glu 
        195                 200                 205             
Pro Ile Tyr Ile Ala Pro Phe Ile Ala Ile Phe Leu Thr Leu Ile Leu 
    210                 215                 220                 
Leu Leu Ile Ser Leu Glu Val Thr Arg Arg Ala Arg Gln Arg Lys Lys 
225                 230                 235                 240 
Ile Leu Lys Gln Ala Met Arg Lys Glu Glu Asn Asn Asp Leu 
                245                 250                 

<210> 101
<211> 197
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> SrtC1

<400> 101
Gln Ser Arg Ala Ile Met Asp Tyr Gln Asp Arg Val Thr His Met Asp 
1               5                   10                  15      
Glu Asn Asp Tyr Lys Lys Ile Ile Asn Arg Ala Lys Glu Tyr Asn Lys 
            20                  25                  30          
Gln Phe Lys Thr Ser Gly Met Lys Trp His Met Thr Ser Gln Glu Arg 
        35                  40                  45              
Leu Asp Tyr Asn Ser Gln Leu Ala Ile Asp Lys Thr Gly Asn Met Gly 
    50                  55                  60                  
Tyr Ile Ser Ile Pro Lys Ile Asn Ile Lys Leu Pro Leu Tyr His Gly 
65                  70                  75                  80  
Thr Ser Glu Lys Val Leu Gln Thr Ser Ile Gly His Leu Glu Gly Ser 
                85                  90                  95      
Ser Leu Pro Ile Gly Gly Asp Ser Thr His Ser Ile Leu Ser Gly His 
            100                 105                 110         
Arg Gly Leu Pro Ser Ser Arg Leu Phe Ser Asp Leu Asp Lys Leu Lys 
        115                 120                 125             
Val Gly Asp His Trp Thr Val Ser Ile Leu Asn Glu Thr Tyr Thr Tyr 
    130                 135                 140                 
Gln Val Asp Gln Ile Arg Thr Val Lys Pro Asp Asp Leu Arg Asp Leu 
145                 150                 155                 160 
Gln Ile Val Lys Gly Lys Asp Tyr Gln Thr Leu Val Thr Cys Thr Pro 
                165                 170                 175     
Tyr Gly Val Asn Thr His Arg Leu Leu Val Arg Gly His Arg Val Pro 
            180                 185                 190         
Asn Asp Asn Gly Asn 
        195         

<210> 102
<211> 254
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> SrtC1

<400> 102
Gln Ser Arg Ala Ile Met Asp Tyr Gln Asp Arg Val Thr His Met Asp 
1               5                   10                  15      
Glu Asn Asp Tyr Lys Lys Ile Ile Asn Arg Ala Lys Glu Tyr Asn Lys 
            20                  25                  30          
Gln Phe Lys Thr Ser Gly Met Lys Ala His Met Thr Ser Gln Glu Arg 
        35                  40                  45              
Leu Asp Tyr Asn Ser Gln Leu Ala Ile Asp Lys Thr Gly Asn Met Gly 
    50                  55                  60                  
Tyr Ile Ser Ile Pro Lys Ile Asn Ile Lys Leu Pro Leu Tyr His Gly 
65                  70                  75                  80  
Thr Ser Glu Lys Val Leu Gln Thr Ser Ile Gly His Leu Glu Gly Ser 
                85                  90                  95      
Ser Leu Pro Ile Gly Gly Asp Ser Thr His Ser Ile Leu Ser Gly His 
            100                 105                 110         
Arg Gly Leu Pro Ser Ser Arg Leu Phe Ser Asp Leu Asp Lys Leu Lys 
        115                 120                 125             
Val Gly Asp His Trp Thr Val Ser Ile Leu Asn Glu Thr Tyr Thr Tyr 
    130                 135                 140                 
Gln Val Asp Gln Ile Arg Thr Val Lys Pro Asp Asp Leu Arg Asp Leu 
145                 150                 155                 160 
Gln Ile Val Lys Gly Lys Asp Tyr Gln Thr Leu Val Thr Cys Thr Pro 
                165                 170                 175     
Tyr Gly Val Asn Thr His Arg Leu Leu Val Arg Gly His Arg Val Pro 
            180                 185                 190         
Asn Asp Asn Gly Asn Ala Leu Val Val Ala Glu Ala Ile Gln Ile Glu 
        195                 200                 205             
Pro Ile Tyr Ile Ala Pro Phe Ile Ala Ile Phe Leu Thr Leu Ile Leu 
    210                 215                 220                 
Leu Leu Ile Ser Leu Glu Val Thr Arg Arg Ala Arg Gln Arg Lys Lys 
225                 230                 235                 240 
Ile Leu Lys Gln Ala Met Arg Lys Glu Glu Asn Asn Asp Leu 
                245                 250                 

<210> 103
<211> 254
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> SrtC1 C188A mutant

<400> 103
Gln Ser Arg Ala Ile Met Asp Tyr Gln Asp Arg Val Thr His Met Asp 
1               5                   10                  15      
Glu Asn Asp Tyr Lys Lys Ile Ile Asn Arg Ala Lys Glu Tyr Asn Lys 
            20                  25                  30          
Gln Phe Lys Thr Ser Gly Met Lys Trp His Met Thr Ser Gln Glu Arg 
        35                  40                  45              
Leu Asp Tyr Asn Ser Gln Leu Ala Ile Asp Lys Thr Gly Asn Met Gly 
    50                  55                  60                  
Tyr Ile Ser Ile Pro Lys Ile Asn Ile Lys Leu Pro Leu Tyr His Gly 
65                  70                  75                  80  
Thr Ser Glu Lys Val Leu Gln Thr Ser Ile Gly His Leu Glu Gly Ser 
                85                  90                  95      
Ser Leu Pro Ile Gly Gly Asp Ser Thr His Ser Ile Leu Ser Gly His 
            100                 105                 110         
Arg Gly Leu Pro Ser Ser Arg Leu Phe Ser Asp Leu Asp Lys Leu Lys 
        115                 120                 125             
Val Gly Asp His Trp Thr Val Ser Ile Leu Asn Glu Thr Tyr Thr Tyr 
    130                 135                 140                 
Gln Val Asp Gln Ile Arg Thr Val Lys Pro Asp Asp Leu Arg Asp Leu 
145                 150                 155                 160 
Gln Ile Val Lys Gly Lys Asp Tyr Gln Thr Leu Val Thr Ala Thr Pro 
                165                 170                 175     
Tyr Gly Val Asn Thr His Arg Leu Leu Val Arg Gly His Arg Val Pro 
            180                 185                 190         
Asn Asp Asn Gly Asn Ala Leu Val Val Ala Glu Ala Ile Gln Ile Glu 
        195                 200                 205             
Pro Ile Tyr Ile Ala Pro Phe Ile Ala Ile Phe Leu Thr Leu Ile Leu 
    210                 215                 220                 
Leu Leu Ile Ser Leu Glu Val Thr Arg Arg Ala Arg Gln Arg Lys Lys 
225                 230                 235                 240 
Ile Leu Lys Gln Ala Met Arg Lys Glu Glu Asn Asn Asp Leu 
                245                 250                 

<210> 104
<211> 807
<212> DNA
<213> Streptococcus agalactiae

<220> 
<221> source
<222> 1..807
<223> /organism="Streptococcus agalactiae"
      /note="SrtC1"
      /mol_type="unassigned DNA"

<400> 104
atggcttatc cttcacttgc taattattgg aattcatttc accaatctcg agcgattatg      60

gattaccaag accgcgtaac gcatatggat gaaaacgatt ataaaaaaat tattaaccga     120

gccaaagaat ataataagca atttaaaact tcaggaatga agtggcacat gactagccaa     180

gagcgtttgg attataattc acaactggct atcgataaaa cgggtaatat gggttatatt     240

tcaattccaa agataaacat aaaattacca ctttatcatg gtacaagtga aaaagtgctt     300

caaacttcta ttggtcattt agaaggaagt agtcttccaa ttggaggaga ctcaactcat     360

tctattttat caggacatag aggtttaccc tcttcaaggc ttttttctga tttggataag     420

ttaaaagttg gagaccactg gacagtcagt atcttaaatg aaacatatac ttatcaagtg     480

gatcaaatca gaacagttaa accggatgat ttgagggatt tacaaattgt taaaggtaaa     540

gactaccaaa ctttggtgac gtgtacacca tatggcgtta atacccatcg gttactagtg     600

agaggacatc gtgtaccaaa cgataatggt aacgctttgg tagtagcaga ggcaatacaa     660

atagagccta tttatatcgc accatttatc gctattttcc ttactttgat tttactttta     720

atctctttag aagtaactag gagagcacgt caacgtaaga aaattttaaa acaagcaatg     780

agaaaggaag agaacaatga tttataa                                         807


<210> 105
<211> 199
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> SrtC2

<400> 105
Met Ile Arg Arg Tyr Ser Ala Asn Phe Leu Ala Ile Leu Gly Ile Ile 
1               5                   10                  15      
Leu Val Ser Ser Gly Ile Tyr Trp Gly Trp Tyr Asn Ile Asn Gln Ala 
            20                  25                  30          
His Gln Ala Asp Leu Thr Ser Gln His Ile Val Lys Val Leu Asp Lys 
        35                  40                  45              
Ser Ile Thr His Gln Val Lys Gly Ser Glu Asn Gly Glu Leu Pro Val 
    50                  55                  60                  
Lys Lys Leu Asp Lys Thr Asp Tyr Leu Gly Thr Leu Asp Ile Pro Asn 
65                  70                  75                  80  
Leu Lys Leu His Leu Pro Val Ala Ala Asn Tyr Ser Phe Glu Gln Leu 
                85                  90                  95      
Ser Lys Thr Pro Thr Arg Tyr Tyr Gly Ser Tyr Leu Thr Asn Asn Met 
            100                 105                 110         
Val Ile Cys Ala His Asn Phe Pro Tyr His Phe Asp Ala Leu Lys Asn 
        115                 120                 125             
Val Asp Met Gly Thr Asp Val Tyr Phe Thr Thr Thr Thr Gly Gln Ile 
    130                 135                 140                 
Tyr His Tyr Lys Ile Ser Asn Arg Glu Ile Ile Glu Pro Thr Ala Ile 
145                 150                 155                 160 
Glu Lys Val Tyr Lys Thr Ala Thr Ser Asp Asn Asp Trp Asp Leu Ser 
                165                 170                 175     
Leu Phe Thr Cys Thr Lys Ala Gly Val Ala Arg Val Leu Val Arg Cys 
            180                 185                 190         
Gln Leu Ile Asp Val Lys Asn 
        195                 

<210> 106
<211> 199
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> SrtC2 Cys substituted with Ala

<400> 106
Met Ile Arg Arg Tyr Ser Ala Asn Phe Leu Ala Ile Leu Gly Ile Ile 
1               5                   10                  15      
Leu Val Ser Ser Gly Ile Tyr Trp Gly Trp Tyr Asn Ile Asn Gln Ala 
            20                  25                  30          
His Gln Ala Asp Leu Thr Ser Gln His Ile Val Lys Val Leu Asp Lys 
        35                  40                  45              
Ser Ile Thr His Gln Val Lys Gly Ser Glu Asn Gly Glu Leu Pro Val 
    50                  55                  60                  
Lys Lys Leu Asp Lys Thr Asp Tyr Leu Gly Thr Leu Asp Ile Pro Asn 
65                  70                  75                  80  
Leu Lys Leu His Leu Pro Val Ala Ala Asn Tyr Ser Phe Glu Gln Leu 
                85                  90                  95      
Ser Lys Thr Pro Thr Arg Tyr Tyr Gly Ser Tyr Leu Thr Asn Asn Met 
            100                 105                 110         
Val Ile Ala Ala His Asn Phe Pro Tyr His Phe Asp Ala Leu Lys Asn 
        115                 120                 125             
Val Asp Met Gly Thr Asp Val Tyr Phe Thr Thr Thr Thr Gly Gln Ile 
    130                 135                 140                 
Tyr His Tyr Lys Ile Ser Asn Arg Glu Ile Ile Glu Pro Thr Ala Ile 
145                 150                 155                 160 
Glu Lys Val Tyr Lys Thr Ala Thr Ser Asp Asn Asp Trp Asp Leu Ser 
                165                 170                 175     
Leu Phe Thr Ala Thr Lys Ala Gly Val Ala Arg Val Leu Val Arg Ala 
            180                 185                 190         
Gln Leu Ile Asp Val Lys Asn 
        195                 

<210> 107
<211> 600
<212> DNA
<213> Streptococcus agalactiae

<220> 
<221> source
<222> 1..600
<223> /organism="Streptococcus agalactiae"
      /note="SrtC2"
      /mol_type="unassigned DNA"

<400> 107
gtgattagaa gatattcagc aaatttttta gctatactcg gaattattct ggtaagttct      60

ggaatctatt ggggttggta taatattaat caggcgcatc aagctgattt aacttctcag     120

catattgtca aggtgcttga taaatctatt acgcatcaag taaagggttc agaaaatgga     180

gaattacctg taaaaaagtt ggataaaaca gattacttgg gaactctgga tattccgaac     240

ttaaaactgc atttaccggt agctgctaat tatagttttg aacaactgtc taagacgcct     300

acaaggtatt atggttctta tttaactaat aacatggtga tttgtgcgca taattttcct     360

tatcattttg atgctttaaa aaatgtagat atgggaacgg atgtttattt tacaactaca     420

acagggcaaa tctatcacta caaaatcagt aatagagaaa ttattgaacc aacagcgatt     480

gaaaaagttt ataaaactgc cacatcagac aatgattggg acttaagctt gtttacttgt     540

acaaaggcag gagtagctag agtattagtg cgctgtcaat taattgatgt taaaaattaa     600


<210> 108
<211> 169
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> SrtC2 No signal peptide

<400> 108
Gln Ala His Gln Ala Asp Leu Thr Ser Gln His Ile Val Lys Val Leu 
1               5                   10                  15      
Asp Lys Ser Ile Thr His Gln Val Lys Gly Ser Glu Asn Gly Glu Leu 
            20                  25                  30          
Pro Val Lys Lys Leu Asp Lys Thr Asp Tyr Leu Gly Thr Leu Asp Ile 
        35                  40                  45              
Pro Asn Leu Lys Leu His Leu Pro Val Ala Ala Asn Tyr Ser Phe Glu 
    50                  55                  60                  
Gln Leu Ser Lys Thr Pro Thr Arg Tyr Tyr Gly Ser Tyr Leu Thr Asn 
65                  70                  75                  80  
Asn Met Val Ile Cys Ala His Asn Phe Pro Tyr His Phe Asp Ala Leu 
                85                  90                  95      
Lys Asn Val Asp Met Gly Thr Asp Val Tyr Phe Thr Thr Thr Thr Gly 
            100                 105                 110         
Gln Ile Tyr His Tyr Lys Ile Ser Asn Arg Glu Ile Ile Glu Pro Thr 
        115                 120                 125             
Ala Ile Glu Lys Val Tyr Lys Thr Ala Thr Ser Asp Asn Asp Trp Asp 
    130                 135                 140                 
Leu Ser Leu Phe Thr Cys Thr Lys Ala Gly Val Ala Arg Val Leu Val 
145                 150                 155                 160 
Arg Cys Gln Leu Ile Asp Val Lys Asn 
                165                 

<210> 109
<211> 190
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> SrtC2 no N-terminal domain

<400> 109
Leu Ala Ile Leu Gly Ile Ile Leu Val Ser Ser Gly Ile Tyr Trp Gly 
1               5                   10                  15      
Trp Tyr Asn Ile Asn Gln Ala His Gln Ala Asp Leu Thr Ser Gln His 
            20                  25                  30          
Ile Val Lys Val Leu Asp Lys Ser Ile Thr His Gln Val Lys Gly Ser 
        35                  40                  45              
Glu Asn Gly Glu Leu Pro Val Lys Lys Leu Asp Lys Thr Asp Tyr Leu 
    50                  55                  60                  
Gly Thr Leu Asp Ile Pro Asn Leu Lys Leu His Leu Pro Val Ala Ala 
65                  70                  75                  80  
Asn Tyr Ser Phe Glu Gln Leu Ser Lys Thr Pro Thr Arg Tyr Tyr Gly 
                85                  90                  95      
Ser Tyr Leu Thr Asn Asn Met Val Ile Cys Ala His Asn Phe Pro Tyr 
            100                 105                 110         
His Phe Asp Ala Leu Lys Asn Val Asp Met Gly Thr Asp Val Tyr Phe 
        115                 120                 125             
Thr Thr Thr Thr Gly Gln Ile Tyr His Tyr Lys Ile Ser Asn Arg Glu 
    130                 135                 140                 
Ile Ile Glu Pro Thr Ala Ile Glu Lys Val Tyr Lys Thr Ala Thr Ser 
145                 150                 155                 160 
Asp Asn Asp Trp Asp Leu Ser Leu Phe Thr Cys Thr Lys Ala Gly Val 
                165                 170                 175     
Ala Arg Val Leu Val Arg Cys Gln Leu Ile Asp Val Lys Asn 
            180                 185                 190 

<210> 110
<211> 502
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS1523/SAN1518 Full length

<400> 110
Met Lys Lys Lys Met Ile Gln Ser Leu Leu Val Ala Ser Leu Ala Phe 
1               5                   10                  15      
Gly Met Ala Val Ser Pro Val Thr Pro Ile Ala Phe Ala Ala Glu Thr 
            20                  25                  30          
Gly Thr Ile Thr Val Gln Asp Thr Gln Lys Gly Ala Thr Tyr Lys Ala 
        35                  40                  45              
Tyr Lys Val Phe Asp Ala Glu Ile Asp Asn Ala Asn Val Ser Asp Ser 
    50                  55                  60                  
Asn Lys Asp Gly Ala Ser Tyr Leu Ile Pro Gln Gly Lys Glu Ala Glu 
65                  70                  75                  80  
Tyr Lys Ala Ser Thr Asp Phe Asn Ser Leu Phe Thr Thr Thr Thr Asn 
                85                  90                  95      
Gly Gly Arg Thr Tyr Val Thr Lys Lys Asp Thr Ala Ser Ala Asn Glu 
            100                 105                 110         
Ile Ala Thr Trp Ala Lys Ser Ile Ser Ala Asn Thr Thr Pro Val Ser 
        115                 120                 125             
Thr Val Thr Glu Ser Asn Asn Asp Gly Thr Glu Val Ile Asn Val Ser 
    130                 135                 140                 
Gln Tyr Gly Tyr Tyr Tyr Val Ser Ser Thr Val Asn Asn Gly Ala Val 
145                 150                 155                 160 
Ile Met Val Thr Ser Val Thr Pro Asn Ala Thr Ile His Glu Lys Asn 
                165                 170                 175     
Thr Asp Ala Thr Trp Gly Asp Gly Gly Gly Lys Thr Val Asp Gln Lys 
            180                 185                 190         
Thr Tyr Ser Val Gly Asp Thr Val Lys Tyr Thr Ile Thr Tyr Lys Asn 
        195                 200                 205             
Ala Val Asn Tyr His Gly Thr Glu Lys Val Tyr Gln Tyr Val Ile Lys 
    210                 215                 220                 
Asp Thr Met Pro Ser Ala Ser Val Val Asp Leu Asn Glu Gly Ser Tyr 
225                 230                 235                 240 
Glu Val Thr Ile Thr Asp Gly Ser Gly Asn Ile Thr Thr Leu Thr Gln 
                245                 250                 255     
Gly Ser Glu Lys Ala Thr Gly Lys Tyr Asn Leu Leu Glu Glu Asn Asn 
            260                 265                 270         
Asn Phe Thr Ile Thr Ile Pro Trp Ala Ala Thr Asn Thr Pro Thr Gly 
        275                 280                 285             
Asn Thr Gln Asn Gly Ala Asn Asp Asp Phe Phe Tyr Lys Gly Ile Asn 
    290                 295                 300                 
Thr Ile Thr Val Thr Tyr Thr Gly Val Leu Lys Ser Gly Ala Lys Pro 
305                 310                 315                 320 
Gly Ser Ala Asp Leu Pro Glu Asn Thr Asn Ile Ala Thr Ile Asn Pro 
                325                 330                 335     
Asn Thr Ser Asn Asp Asp Pro Gly Gln Lys Val Thr Val Arg Asp Gly 
            340                 345                 350         
Gln Ile Thr Ile Lys Lys Ile Asp Gly Ser Thr Lys Ala Ser Leu Gln 
        355                 360                 365             
Gly Ala Ile Phe Val Leu Lys Asn Ala Thr Gly Gln Phe Leu Asn Phe 
    370                 375                 380                 
Asn Asp Thr Asn Asn Val Glu Trp Gly Thr Glu Ala Asn Ala Thr Glu 
385                 390                 395                 400 
Tyr Thr Thr Gly Ala Asp Gly Ile Ile Thr Ile Thr Gly Leu Lys Glu 
                405                 410                 415     
Gly Thr Tyr Tyr Leu Val Glu Lys Lys Ala Pro Leu Gly Tyr Asn Leu 
            420                 425                 430         
Leu Asp Asn Ser Gln Lys Val Ile Leu Gly Asp Gly Ala Thr Asp Thr 
        435                 440                 445             
Thr Asn Ser Asp Asn Leu Leu Val Asn Pro Thr Val Glu Asn Asn Lys 
    450                 455                 460                 
Gly Thr Glu Leu Pro Ser Thr Gly Gly Ile Gly Thr Thr Ile Phe Tyr 
465                 470                 475                 480 
Ile Ile Gly Ala Ile Leu Val Ile Gly Ala Gly Ile Val Leu Val Ala 
                485                 490                 495     
Arg Arg Arg Leu Arg Ser 
            500         

<210> 111
<211> 438
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS1523/SAN1518 Q41K mutation

<400> 111
Ala Glu Thr Gly Thr Ile Thr Val Gln Asp Thr Gln Lys Gly Ala Thr 
1               5                   10                  15      
Tyr Lys Ala Tyr Lys Val Phe Asp Ala Glu Ile Asp Asn Ala Asn Val 
            20                  25                  30          
Ser Asp Ser Asn Lys Asp Gly Ala Ser Tyr Leu Ile Pro Gln Gly Lys 
        35                  40                  45              
Glu Ala Glu Tyr Lys Ala Ser Thr Asp Phe Asn Ser Leu Phe Thr Thr 
    50                  55                  60                  
Thr Thr Asn Gly Gly Arg Thr Tyr Val Thr Lys Lys Asp Thr Ala Ser 
65                  70                  75                  80  
Ala Asn Glu Ile Ala Thr Trp Ala Lys Ser Ile Ser Ala Asn Thr Thr 
                85                  90                  95      
Pro Val Ser Thr Val Thr Glu Ser Asn Asn Asp Gly Thr Glu Val Ile 
            100                 105                 110         
Asn Val Ser Gln Tyr Gly Tyr Tyr Tyr Val Ser Ser Thr Val Asn Asn 
        115                 120                 125             
Gly Ala Val Ile Met Val Thr Ser Val Thr Pro Asn Ala Thr Ile His 
    130                 135                 140                 
Glu Lys Asn Thr Asp Ala Thr Trp Gly Asp Gly Gly Gly Lys Thr Val 
145                 150                 155                 160 
Asp Gln Lys Thr Tyr Ser Val Gly Asp Thr Val Lys Tyr Thr Ile Thr 
                165                 170                 175     
Tyr Lys Asn Ala Val Asn Tyr His Gly Thr Glu Lys Val Tyr Gln Tyr 
            180                 185                 190         
Val Ile Lys Asp Thr Met Pro Ser Ala Ser Val Val Asp Leu Asn Glu 
        195                 200                 205             
Gly Ser Tyr Glu Val Thr Ile Thr Asp Gly Ser Gly Asn Ile Thr Thr 
    210                 215                 220                 
Leu Thr Gln Gly Ser Glu Lys Ala Thr Gly Lys Tyr Asn Leu Leu Glu 
225                 230                 235                 240 
Glu Asn Asn Asn Phe Thr Ile Thr Ile Pro Trp Ala Ala Thr Asn Thr 
                245                 250                 255     
Pro Thr Gly Asn Thr Gln Asn Gly Ala Asn Asp Asp Phe Phe Tyr Lys 
            260                 265                 270         
Gly Ile Asn Thr Ile Thr Val Thr Tyr Thr Gly Val Leu Lys Ser Gly 
        275                 280                 285             
Ala Lys Pro Gly Ser Ala Asp Leu Pro Glu Asn Thr Asn Ile Ala Thr 
    290                 295                 300                 
Ile Asn Pro Asn Thr Ser Asn Asp Asp Pro Gly Gln Lys Val Thr Val 
305                 310                 315                 320 
Arg Asp Gly Gln Ile Thr Ile Lys Lys Ile Asp Gly Ser Thr Lys Ala 
                325                 330                 335     
Ser Leu Gln Gly Ala Ile Phe Val Leu Lys Asn Ala Thr Gly Gln Phe 
            340                 345                 350         
Leu Asn Phe Asn Asp Thr Asn Asn Val Glu Trp Gly Thr Glu Ala Asn 
        355                 360                 365             
Ala Thr Glu Tyr Thr Thr Gly Ala Asp Gly Ile Ile Thr Ile Thr Gly 
    370                 375                 380                 
Leu Lys Glu Gly Thr Tyr Tyr Leu Val Glu Lys Lys Ala Pro Leu Gly 
385                 390                 395                 400 
Tyr Asn Leu Leu Asp Asn Ser Gln Lys Val Ile Leu Gly Asp Gly Ala 
                405                 410                 415     
Thr Asp Thr Thr Asn Ser Asp Asn Leu Leu Val Asn Pro Thr Val Glu 
            420                 425                 430         
Asn Asn Lys Gly Thr Glu 
        435             

<210> 112
<211> 438
<212> PRT
<213> Streptococcus agalactiae

<220> 
<223> GBS1523/SAN1518 No signal sequence Q41K mutation

<400> 112
Ala Glu Thr Gly Thr Ile Thr Val Gln Asp Thr Lys Lys Gly Ala Thr 
1               5                   10                  15      
Tyr Lys Ala Tyr Lys Val Phe Asp Ala Glu Ile Asp Asn Ala Asn Val 
            20                  25                  30          
Ser Asp Ser Asn Lys Asp Gly Ala Ser Tyr Leu Ile Pro Gln Gly Lys 
        35                  40                  45              
Glu Ala Glu Tyr Lys Ala Ser Thr Asp Phe Asn Ser Leu Phe Thr Thr 
    50                  55                  60                  
Thr Thr Asn Gly Gly Arg Thr Tyr Val Thr Lys Lys Asp Thr Ala Ser 
65                  70                  75                  80  
Ala Asn Glu Ile Ala Thr Trp Ala Lys Ser Ile Ser Ala Asn Thr Thr 
                85                  90                  95      
Pro Val Ser Thr Val Thr Glu Ser Asn Asn Asp Gly Thr Glu Val Ile 
            100                 105                 110         
Asn Val Ser Gln Tyr Gly Tyr Tyr Tyr Val Ser Ser Thr Val Asn Asn 
        115                 120                 125             
Gly Ala Val Ile Met Val Thr Ser Val Thr Pro Asn Ala Thr Ile His 
    130                 135                 140                 
Glu Lys Asn Thr Asp Ala Thr Trp Gly Asp Gly Gly Gly Lys Thr Val 
145                 150                 155                 160 
Asp Gln Lys Thr Tyr Ser Val Gly Asp Thr Val Lys Tyr Thr Ile Thr 
                165                 170                 175     
Tyr Lys Asn Ala Val Asn Tyr His Gly Thr Glu Lys Val Tyr Gln Tyr 
            180                 185                 190         
Val Ile Lys Asp Thr Met Pro Ser Ala Ser Val Val Asp Leu Asn Glu 
        195                 200                 205             
Gly Ser Tyr Glu Val Thr Ile Thr Asp Gly Ser Gly Asn Ile Thr Thr 
    210                 215                 220                 
Leu Thr Gln Gly Ser Glu Lys Ala Thr Gly Lys Tyr Asn Leu Leu Glu 
225                 230                 235                 240 
Glu Asn Asn Asn Phe Thr Ile Thr Ile Pro Trp Ala Ala Thr Asn Thr 
                245                 250                 255     
Pro Thr Gly Asn Thr Gln Asn Gly Ala Asn Asp Asp Phe Phe Tyr Lys 
            260                 265                 270         
Gly Ile Asn Thr Ile Thr Val Thr Tyr Thr Gly Val Leu Lys Ser Gly 
        275                 280                 285             
Ala Lys Pro Gly Ser Ala Asp Leu Pro Glu Asn Thr Asn Ile Ala Thr 
    290                 295                 300                 
Ile Asn Pro Asn Thr Ser Asn Asp Asp Pro Gly Gln Lys Val Thr Val 
305                 310                 315                 320 
Arg Asp Gly Gln Ile Thr Ile Lys Lys Ile Asp Gly Ser Thr Lys Ala 
                325                 330                 335     
Ser Leu Gln Gly Ala Ile Phe Val Leu Lys Asn Ala Thr Gly Gln Phe 
            340                 345                 350         
Leu Asn Phe Asn Asp Thr Asn Asn Val Glu Trp Gly Thr Glu Ala Asn 
        355                 360                 365             
Ala Thr Glu Tyr Thr Thr Gly Ala Asp Gly Ile Ile Thr Ile Thr Gly 
    370                 375                 380                 
Leu Lys Glu Gly Thr Tyr Tyr Leu Val Glu Lys Lys Ala Pro Leu Gly 
385                 390                 395                 400 
Tyr Asn Leu Leu Asp Asn Ser Gln Lys Val Ile Leu Gly Asp Gly Ala 
                405                 410                 415     
Thr Asp Thr Thr Asn Ser Asp Asn Leu Leu Val Asn Pro Thr Val Glu 
            420                 425                 430         
Asn Asn Lys Gly Thr Glu 
        435             

<210> 113
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..48
<223> /organism="Artificial Sequence"
      /note="GFP-LPxTG Forward PCR Primer"
      /mol_type="unassigned DNA"

<400> 113
attccacaaa caggtggtat tggtacataa cgcgacttaa ttaaacgg                  48


<210> 114
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..48
<223> /organism="Artificial Sequence"
      /note="GFP-LPxTG Reverse PCR Primer"
      /mol_type="unassigned DNA"

<400> 114
tgtaccaata ccacctgttt gtggaatctt gtacagctcg tccatgcc                  48


<210> 115
<211> 880
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..880
<223> /organism="Artificial Sequence"
      /note="EGFP DNA sequence"
      /mol_type="unassigned DNA"

<400> 115
ctttaagaag gagatataca tacccatggg atctgataaa attcatcatc atcatcatca      60

cgaaaacctg tacttccagg gcatggtgag caagggcgag gagctgttca ccggggtggt     120

gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga     180

gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa     240

gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag     300

ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta     360

cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt     420

gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga     480

ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat     540

catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga     600

ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc     660

cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa     720

cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg     780

catggacgag ctgtacaagt aacgcgactt aattaaacgg tctccagctt ggctgttttg     840

gcggatgaga gaagattttc agcctgatac agattaaatc                           880


<210> 116
<211> 258
<212> PRT
<213> Artificial Sequence

<220> 
<223> EGFP Amino Acid Sequence

<400> 116
Met Gly Ser Asp Lys Ile His His His His His His Glu Asn Leu Tyr 
1               5                   10                  15      
Phe Gln Gly Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val 
            20                  25                  30          
Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser 
        35                  40                  45              
Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu 
    50                  55                  60                  
Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu 
65                  70                  75                  80  
Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp 
                85                  90                  95      
His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr 
            100                 105                 110         
Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr 
        115                 120                 125             
Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu 
    130                 135                 140                 
Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys 
145                 150                 155                 160 
Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys 
                165                 170                 175     
Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu 
            180                 185                 190         
Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile 
        195                 200                 205             
Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln 
    210                 215                 220                 
Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu 
225                 230                 235                 240 
Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 
                245                 250                 255     
Tyr Lys 
        

<210> 117
<211> 907
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..907
<223> /organism="Artificial Sequence"
      /note="GFP comprising LPxTG tag"
      /mol_type="unassigned DNA"

<400> 117
ctttaagaag gagatataca tacccatggg atctgataaa attcatcatc atcatcatca      60

cgaaaacctg tacttccagg gcatggtgag caagggcgag gagctgttca ccggggtggt     120

gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga     180

gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa     240

gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag     300

ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta     360

cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt     420

gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga     480

ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat     540

catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga     600

ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc     660

cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa     720

cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg     780

catggacgag ctgtacaaga ttccacaaac aggtggtatt ggtacataac gcgacttaat     840

taaacggtct ccagcttggc tgttttggcg gatgagagaa gattttcagc ctgatacaga     900

ttaaatc                                                               907


<210> 118
<211> 267
<212> PRT
<213> Artificial Sequence

<220> 
<223> GFP comprising LPxTG sequence tag

<400> 118
Met Gly Ser Asp Lys Ile His His His His His His Glu Asn Leu Tyr 
1               5                   10                  15      
Phe Gln Gly Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val 
            20                  25                  30          
Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser 
        35                  40                  45              
Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu 
    50                  55                  60                  
Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu 
65                  70                  75                  80  
Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp 
                85                  90                  95      
His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr 
            100                 105                 110         
Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr 
        115                 120                 125             
Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu 
    130                 135                 140                 
Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys 
145                 150                 155                 160 
Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys 
                165                 170                 175     
Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu 
            180                 185                 190         
Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile 
        195                 200                 205             
Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln 
    210                 215                 220                 
Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu 
225                 230                 235                 240 
Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 
                245                 250                 255     
Tyr Lys Ile Pro Gln Thr Gly Gly Ile Gly Thr 
            260                 265         

