                         SEQUENCE LISTING

<110>  The Regents of University of California
       Nivala, Jeffrey
       Marks, Douglas
       Akeson, Mark
 
<120>  Nanopore Sensor for Enzyme-Mediated Protein Translocation

<130>  482.34-1 PCT

<150>  US 61/599,754
<151>  2012-02-16

<150>  US 61/713,163
<151>  2012-10-12

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  76
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  1

Gly Gly Ser Ser Gly Gly Ser Gly Gly Ser Gly Ser Ser Gly Asp Gly 
1               5                   10                  15      


Gly Ser Ser Gly Gly Ser Gly Gly Ser Gly Ser Ser Gly Asp Gly Gly 
            20                  25                  30          


Ser Ser Gly Gly Ser Gly Gly Asp Gly Ser Ser Gly Asp Gly Gly Ser 
        35                  40                  45              


Asp Gly Asp Ser Asp Gly Ser Asp Gly Asp Gly Asp Ser Asp Gly Asp 
    50                  55                  60                  


Asp Ala Ala Asn Asp Glu Asn Tyr Ala Leu Ala Ala 
65                  70                  75      


<210>  2
<211>  35
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  2

Gly Gly Ser Gly Ser Gly Gly Ser Gly Ser Gly Gly Ser Gly Ser Gln 
1               5                   10                  15      


Asn Glu Tyr Arg Ser Gly Gly Ser Gly Ser Gly Gly Ser Gly Ser Gly 
            20                  25                  30          


Gly Ser Gly 
        35  


<210>  3
<211>  120
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  3

Gly Gly Ser Gly Ser Ala Gly Ser Gly Ala Ser Gly Ser Ser Gly Ser 
1               5                   10                  15      


Glu Gly Ser Gly Ala Ser Gly Ser Ala Gly Ser Gly Ser Ala Gly Ser 
            20                  25                  30          


Arg Gly Ser Gly Ala Ser Gly Ser Ala Gly Ser Gly Ser Ala Gly Ser 
        35                  40                  45              


Gly Gly Ala Glu Ala Ala Lys Glu Ala Ala Lys Glu Ala Ala Lys Glu 
    50                  55                  60                  


Ala Ala Lys Glu Ala Ala Lys Ala Gly Gly Ser Gly Ser Ala Gly Ser 
65                  70                  75                  80  


Ala Gly Ser Ala Ser Ser Gly Ser Asp Gly Ser Gly Ala Ser Gly Ser 
                85                  90                  95      


Ala Gly Ser Gly Ser Ala Gly Ser Lys Gly Ser Gly Ala Ser Gly Ser 
            100                 105                 110         


Ala Gly Ser Gly Ser Ser Gly Ser 
        115                 120 


<210>  4
<211>  195
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  4

Met Gly Ser Ser His His His His His His Gly Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser Ala Ser Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys 
            20                  25                  30          


Pro Glu Val Lys Pro Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys 
        35                  40                  45              


Val Ser Asp Gly Ser Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr 
    50                  55                  60                  


Pro Leu Arg Arg Leu Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu 
65                  70                  75                  80  


Met Asp Ser Leu Arg Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp 
                85                  90                  95      


Gln Thr Pro Glu Asp Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala 
            100                 105                 110         


His Arg Glu Gln Ile Gly Gly Gly Gly Ser Ser Gly Gly Ser Gly Gly 
        115                 120                 125             


Ser Gly Ser Ser Gly Asp Gly Gly Ser Ser Gly Gly Ser Gly Gly Ser 
    130                 135                 140                 


Gly Ser Ser Gly Asp Gly Gly Ser Ser Gly Gly Ser Gly Gly Asp Gly 
145                 150                 155                 160 


Ser Ser Gly Asp Gly Gly Ser Asp Gly Asp Ser Asp Gly Ser Asp Gly 
                165                 170                 175     


Asp Gly Asp Ser Asp Gly Asp Asp Ala Ala Asn Asp Glu Asn Tyr Ala 
            180                 185                 190         


Leu Ala Ala 
        195 


<210>  5
<211>  338
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  5

Met Gly His His His His His His Gly Ser Leu Gln Asp Ser Glu Val 
1               5                   10                  15      


Asn Gln Glu Ala Lys Pro Glu Val Lys Pro Glu Val Lys Pro Glu Thr 
            20                  25                  30          


His Ile Asn Leu Lys Val Ser Asp Gly Ser Ser Glu Ile Phe Phe Lys 
        35                  40                  45              


Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu Met Glu Ala Phe Ala Lys 
    50                  55                  60                  


Arg Gln Gly Lys Glu Met Asp Ser Leu Arg Phe Leu Tyr Asp Gly Ile 
65                  70                  75                  80  


Arg Ile Gln Ala Asp Gln Ala Pro Glu Asp Leu Asp Met Glu Asp Asn 
                85                  90                  95      


Asp Ile Ile Glu Ala His Arg Glu Gln Ile Gly Gly Gly Gly Ser Gly 
            100                 105                 110         


Ser Gly Gly Ser Gly Ser Gly Gly Ser Gly Ser Gln Asn Glu Tyr Arg 
        115                 120                 125             


Ser Gly Gly Ser Gly Ser Gly Gly Ser Gly Ser Gly Gly Ser Gly Met 
    130                 135                 140                 


Gly Ser Ser His His His His His His Gly Ser Gly Leu Val Pro Arg 
145                 150                 155                 160 


Gly Ser Ala Ser Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys Pro 
                165                 170                 175     


Glu Val Lys Pro Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys Val 
            180                 185                 190         


Ser Asp Gly Ser Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro 
        195                 200                 205             


Leu Arg Arg Leu Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu Met 
    210                 215                 220                 


Asp Ser Leu Arg Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln 
225                 230                 235                 240 


Thr Pro Glu Asp Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala His 
                245                 250                 255     


Arg Glu Gln Ile Gly Gly Gly Gly Ser Ser Gly Gly Ser Gly Gly Ser 
            260                 265                 270         


Gly Ser Ser Gly Asp Gly Gly Ser Ser Gly Gly Ser Gly Gly Ser Gly 
        275                 280                 285             


Ser Ser Gly Asp Gly Gly Ser Ser Gly Gly Ser Gly Gly Asp Gly Ser 
    290                 295                 300                 


Ser Gly Asp Gly Gly Ser Asp Gly Asp Ser Asp Gly Ser Asp Gly Asp 
305                 310                 315                 320 


Gly Asp Ser Asp Gly Asp Asp Ala Ala Asn Asp Glu Asn Tyr Ala Leu 
                325                 330                 335     


Ala Ala 
        


<210>  6
<211>  451
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  6

Met Gly His His His His His His Gly Ser Leu Gln Asp Ser Glu Val 
1               5                   10                  15      


Asn Gln Glu Ala Lys Pro Glu Val Lys Pro Glu Val Lys Pro Glu Thr 
            20                  25                  30          


His Ile Asn Leu Lys Val Ser Asp Gly Ser Ser Glu Ile Phe Phe Lys 
        35                  40                  45              


Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu Met Glu Ala Phe Ala Lys 
    50                  55                  60                  


Arg Gln Gly Lys Glu Met Asp Ser Leu Arg Phe Leu Tyr Asp Gly Ile 
65                  70                  75                  80  


Arg Ile Gln Ala Asp Gln Ala Pro Glu Asp Leu Asp Met Glu Asp Asn 
                85                  90                  95      


Asp Ile Ile Glu Ala His Arg Glu Gln Ile Gly Gly Gly Gly Ser Gly 
            100                 105                 110         


Ser Gly Gly Ser Gly Ser Gly Gly Ser Gly Ser Gln Asn Glu Tyr Arg 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Ser Ala Gly Ser Gly Ala Ser Gly Ser 
    130                 135                 140                 


Ser Gly Ser Glu Gly Ser Gly Ala Ser Gly Ser Ala Gly Ser Gly Ser 
145                 150                 155                 160 


Ala Gly Ser Arg Gly Ser Gly Ala Ser Gly Ser Ala Gly Ser Gly Ser 
                165                 170                 175     


Ala Gly Ser Gly Gly Ala Glu Ala Ala Lys Glu Ala Ala Lys Glu Ala 
            180                 185                 190         


Ala Lys Glu Ala Ala Lys Glu Ala Ala Lys Ala Gly Gly Ser Gly Ser 
        195                 200                 205             


Ala Gly Ser Ala Gly Ser Ala Ser Ser Gly Ser Asp Gly Ser Gly Ala 
    210                 215                 220                 


Ser Gly Ser Ala Gly Ser Gly Ser Ala Gly Ser Lys Gly Ser Gly Ala 
225                 230                 235                 240 


Ser Gly Ser Ala Gly Ser Gly Ser Ser Gly Ser Ser Gly Gly Ser Gly 
                245                 250                 255     


Met Gly Ser Ser His His His His His His Gly Ser Gly Leu Val Pro 
            260                 265                 270         


Arg Gly Ser Ala Ser Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys 
        275                 280                 285             


Pro Glu Val Lys Pro Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys 
    290                 295                 300                 


Val Ser Asp Gly Ser Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr 
305                 310                 315                 320 


Pro Leu Arg Arg Leu Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu 
                325                 330                 335     


Met Asp Ser Leu Arg Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp 
            340                 345                 350         


Gln Thr Pro Glu Asp Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala 
        355                 360                 365             


His Arg Glu Gln Ile Gly Gly Gly Gly Ser Ser Gly Gly Ser Gly Gly 
    370                 375                 380                 


Ser Gly Ser Ser Gly Asp Gly Gly Ser Ser Gly Gly Ser Gly Gly Ser 
385                 390                 395                 400 


Gly Ser Ser Gly Asp Gly Gly Ser Ser Gly Gly Ser Gly Gly Asp Gly 
                405                 410                 415     


Ser Ser Gly Asp Gly Gly Ser Asp Gly Asp Ser Asp Gly Ser Asp Gly 
            420                 425                 430         


Asp Gly Asp Ser Asp Gly Asp Asp Ala Ala Asn Asp Glu Asn Tyr Ala 
        435                 440                 445             


Leu Ala Ala 
    450     


<210>  7
<211>  198
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic

<400>  7

Met Gly Ser Ser His His His His His His Gly Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser Ala Ser Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys 
            20                  25                  30          


Pro Glu Val Lys Pro Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys 
        35                  40                  45              


Val Ser Asp Gly Ser Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr 
    50                  55                  60                  


Pro Leu Arg Arg Leu Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu 
65                  70                  75                  80  


Met Asp Ser Leu Arg Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp 
                85                  90                  95      


Gln Thr Pro Glu Asp Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala 
            100                 105                 110         


His Arg Glu Gln Ile Gly Gly Gly Gly Ser Ser Gly Gly Ser Gly Gly 
        115                 120                 125             


Ser Gly Ser Ser Gly Asp Gly Gly Ser Ser Gly Gly Ser Gly Gly Ser 
    130                 135                 140                 


Gly Ser Ser Gly Asp Gly Gly Ser Ser Gly Gly Ser Gly Gly Asp Gly 
145                 150                 155                 160 


Ser Ser Gly Asp Gly Gly Ser Asp Gly Asp Ser Asp Gly Ser Asp Gly 
                165                 170                 175     


Asp Gly Asp Ser Asp Gly Asp Asp Ala Ala Asn Asp Glu Asn Tyr Ala 
            180                 185                 190         


Leu Ala Ala Arg Gln Ala 
        195             


<210>  8
<211>  1145
<212>  PRT
<213>  artificial

<220>
<223>  synthetic

<400>  8

Met Gly Ser Ser His His His His His His Ser Ser His Met Ser Ala 
1               5                   10                  15      


Leu Pro Thr Pro His Glu Ile Arg Asn His Leu Asp Asp Tyr Val Ile 
            20                  25                  30          


Gly Gln Glu Gln Ala Lys Lys Val Leu Ala Val Ala Val Tyr Asn His 
        35                  40                  45              


Tyr Lys Arg Leu Arg Asn Gly Asp Thr Ser Asn Gly Val Glu Leu Gly 
    50                  55                  60                  


Lys Ser Asn Ile Leu Leu Ile Gly Pro Thr Gly Ser Gly Lys Thr Leu 
65                  70                  75                  80  


Leu Ala Glu Thr Leu Ala Arg Leu Leu Asp Val Pro Phe Thr Met Ala 
                85                  90                  95      


Asp Ala Thr Thr Leu Thr Glu Ala Gly Tyr Val Gly Glu Asp Val Glu 
            100                 105                 110         


Asn Ile Ile Gln Lys Leu Leu Gln Lys Cys Asp Tyr Asp Val Gln Lys 
        115                 120                 125             


Ala Gln Arg Gly Ile Val Tyr Ile Asp Glu Ile Asp Lys Ile Ser Arg 
    130                 135                 140                 


Lys Ser Asp Asn Pro Ser Ile Thr Arg Asp Val Ser Gly Glu Gly Val 
145                 150                 155                 160 


Gln Gln Ala Leu Leu Lys Leu Ile Glu Gly Thr Val Ala Ala Val Pro 
                165                 170                 175     


Pro Gln Gly Gly Arg Lys His Pro Gln Gln Glu Phe Leu Gln Val Asp 
            180                 185                 190         


Thr Ser Lys Ile Leu Phe Ile Cys Gly Gly Ala Phe Ala Gly Leu Asp 
        195                 200                 205             


Lys Val Ile Ser His Arg Val Glu Thr Gly Ser Gly Ile Gly Phe Gly 
    210                 215                 220                 


Ala Thr Val Lys Ala Lys Ser Asp Lys Ala Ser Glu Gly Glu Leu Leu 
225                 230                 235                 240 


Ala Gln Val Glu Pro Glu Asp Leu Ile Lys Phe Gly Leu Ile Pro Glu 
                245                 250                 255     


Phe Ile Gly Arg Leu Pro Val Val Ala Thr Leu Asn Glu Leu Ser Glu 
            260                 265                 270         


Glu Ala Leu Ile Gln Ile Leu Lys Glu Pro Lys Asn Ala Leu Thr Lys 
        275                 280                 285             


Gln Tyr Gln Ala Leu Phe Asn Leu Glu Gly Val Asp Leu Glu Phe Arg 
    290                 295                 300                 


Asp Glu Ala Leu Asp Ala Ile Ala Lys Lys Ala Met Ala Arg Lys Thr 
305                 310                 315                 320 


Gly Ala Arg Gly Leu Arg Ser Ile Val Glu Ala Ala Leu Leu Asp Thr 
                325                 330                 335     


Met Tyr Asp Leu Pro Ser Met Glu Asp Val Glu Lys Val Val Ile Asp 
            340                 345                 350         


Glu Ser Val Ile Asp Gly Gln Ser Lys Pro Leu Leu Ile Tyr Gly Lys 
        355                 360                 365             


Pro Glu Ala Gln Gln Ala Ser Gly Glu Ala Ser Gly Ala Gly Gly Ser 
    370                 375                 380                 


Glu Gly Gly Gly Ser Glu Gly Gly Thr Ser Gly Ala Thr Met Ser Ala 
385                 390                 395                 400 


Leu Pro Thr Pro His Glu Ile Arg Asn His Leu Asp Asp Tyr Val Ile 
                405                 410                 415     


Gly Gln Glu Gln Ala Lys Lys Val Leu Ala Val Ala Val Tyr Asn His 
            420                 425                 430         


Tyr Lys Arg Leu Arg Asn Gly Asp Thr Ser Asn Gly Val Glu Leu Gly 
        435                 440                 445             


Lys Ser Asn Ile Leu Leu Ile Gly Pro Thr Gly Ser Gly Lys Thr Leu 
    450                 455                 460                 


Leu Ala Glu Thr Leu Ala Arg Leu Leu Asp Val Pro Phe Thr Met Ala 
465                 470                 475                 480 


Asp Ala Thr Thr Leu Thr Glu Ala Gly Tyr Val Gly Glu Asp Val Glu 
                485                 490                 495     


Asn Ile Ile Gln Lys Leu Leu Gln Lys Cys Asp Tyr Asp Val Gln Lys 
            500                 505                 510         


Ala Gln Arg Gly Ile Val Tyr Ile Asp Glu Ile Asp Lys Ile Ser Arg 
        515                 520                 525             


Lys Ser Asp Asn Pro Ser Ile Thr Arg Asp Val Ser Gly Glu Gly Val 
    530                 535                 540                 


Gln Gln Ala Leu Leu Lys Leu Ile Glu Gly Thr Val Ala Ala Val Pro 
545                 550                 555                 560 


Pro Gln Gly Gly Arg Lys His Pro Gln Gln Glu Phe Leu Gln Val Asp 
                565                 570                 575     


Thr Ser Lys Ile Leu Phe Ile Cys Gly Gly Ala Phe Ala Gly Leu Asp 
            580                 585                 590         


Lys Val Ile Ser His Arg Val Glu Thr Gly Ser Gly Ile Gly Phe Gly 
        595                 600                 605             


Ala Thr Val Lys Ala Lys Ser Asp Lys Ala Ser Glu Gly Glu Leu Leu 
    610                 615                 620                 


Ala Gln Val Glu Pro Glu Asp Leu Ile Lys Phe Gly Leu Ile Pro Glu 
625                 630                 635                 640 


Phe Ile Gly Arg Leu Pro Val Val Ala Thr Leu Asn Glu Leu Ser Glu 
                645                 650                 655     


Glu Ala Leu Ile Gln Ile Leu Lys Glu Pro Lys Asn Ala Leu Thr Lys 
            660                 665                 670         


Gln Tyr Gln Ala Leu Phe Asn Leu Glu Gly Val Asp Leu Glu Phe Arg 
        675                 680                 685             


Asp Glu Ala Leu Asp Ala Ile Ala Lys Lys Ala Met Ala Arg Lys Thr 
    690                 695                 700                 


Gly Ala Arg Gly Leu Arg Ser Ile Val Glu Ala Ala Leu Leu Asp Thr 
705                 710                 715                 720 


Met Tyr Asp Leu Pro Ser Met Glu Asp Val Glu Lys Val Val Ile Asp 
                725                 730                 735     


Glu Ser Val Ile Asp Gly Gln Ser Lys Pro Leu Leu Ile Tyr Gly Lys 
            740                 745                 750         


Pro Glu Ala Gln Gln Ala Ser Gly Glu Ala Ser Gly Ala Gly Gly Ser 
        755                 760                 765             


Glu Gly Gly Gly Ser Glu Gly Gly Ser Ser Gly Ala Thr Met Ser Ala 
    770                 775                 780                 


Leu Pro Thr Pro His Glu Ile Arg Asn His Leu Asp Asp Tyr Val Ile 
785                 790                 795                 800 


Gly Gln Glu Gln Ala Lys Lys Val Leu Ala Val Ala Val Tyr Asn His 
                805                 810                 815     


Tyr Lys Arg Leu Arg Asn Gly Asp Thr Ser Asn Gly Val Glu Leu Gly 
            820                 825                 830         


Lys Ser Asn Ile Leu Leu Ile Gly Pro Thr Gly Ser Gly Lys Thr Leu 
        835                 840                 845             


Leu Ala Glu Thr Leu Ala Arg Leu Leu Asp Val Pro Phe Thr Met Ala 
    850                 855                 860                 


Asp Ala Thr Thr Leu Thr Glu Ala Gly Tyr Val Gly Glu Asp Val Glu 
865                 870                 875                 880 


Asn Ile Ile Gln Lys Leu Leu Gln Lys Cys Asp Tyr Asp Val Gln Lys 
                885                 890                 895     


Ala Gln Arg Gly Ile Val Tyr Ile Asp Glu Ile Asp Lys Ile Ser Arg 
            900                 905                 910         


Lys Ser Asp Asn Pro Ser Ile Thr Arg Asp Val Ser Gly Glu Gly Val 
        915                 920                 925             


Gln Gln Ala Leu Leu Lys Leu Ile Glu Gly Thr Val Ala Ala Val Pro 
    930                 935                 940                 


Pro Gln Gly Gly Arg Lys His Pro Gln Gln Glu Phe Leu Gln Val Asp 
945                 950                 955                 960 


Thr Ser Lys Ile Leu Phe Ile Cys Gly Gly Ala Phe Ala Gly Leu Asp 
                965                 970                 975     


Lys Val Ile Ser His Arg Val Glu Thr Gly Ser Gly Ile Gly Phe Gly 
            980                 985                 990         


Ala Thr Val Lys Ala Lys Ser Asp  Lys Ala Ser Glu Gly  Glu Leu Leu 
        995                 1000                 1005             


Ala Gln  Val Glu Pro Glu Asp  Leu Ile Lys Phe Gly  Leu Ile Pro 
    1010                 1015                 1020             


Glu Phe  Ile Gly Arg Leu Pro  Val Val Ala Thr Leu  Asn Glu Leu 
    1025                 1030                 1035             


Ser Glu  Glu Ala Leu Ile Gln  Ile Leu Lys Glu Pro  Lys Asn Ala 
    1040                 1045                 1050             


Leu Thr  Lys Gln Tyr Gln Ala  Leu Phe Asn Leu Glu  Gly Val Asp 
    1055                 1060                 1065             


Leu Glu  Phe Arg Asp Glu Ala  Leu Asp Ala Ile Ala  Lys Lys Ala 
    1070                 1075                 1080             


Met Ala  Arg Lys Thr Gly Ala  Arg Gly Leu Arg Ser  Ile Val Glu 
    1085                 1090                 1095             


Ala Ala  Leu Leu Asp Thr Met  Tyr Asp Leu Pro Ser  Met Glu Asp 
    1100                 1105                 1110             


Val Glu  Lys Val Val Ile Asp  Glu Ser Val Ile Asp  Gly Gln Ser 
    1115                 1120                 1125             


Lys Pro  Leu Leu Ile Tyr Gly  Lys Pro Glu Ala Gln  Gln Ala Ser 
    1130                 1135                 1140             


Gly Glu  
    1145 


