                         SEQUENCE LISTING

<110>  Hamilton, Stephen R.
 
<120>  METHODS FOR REDUCING MANNOSYLTRANSFERASE ACTIVITY IN YEAST

<130>  23193

<150>  61/599,616
<151>  2012-02-16

<160>  77    

<170>  PatentIn version 3.5

<210>  1
<211>  968
<212>  DNA
<213>  Artificial

<220>
<223>  Pichia pastoris VRG4 5' knock-out region

<400>  1
gcgagctcgt ctacaaacag tatcactgtt cgtcaggatc cagaaatcga acgggagatg       60

gagttgaaaa ggcagcaaga agaacaggaa aagatggaac tgactgatat gatcaagact      120

gctctacgag accaggtaga gcatatgcct gctgccaaaa cgatcgatgt taacaaaatg      180

acgacagaag atttgctaaa ctggcacctg ggagatcagt ccacaaaaaa cagttctatc      240

catcgtgaat ttgatccatc agagcaagaa gagtttaata ggctagcaca aaagattgcc      300

aaagttaaga taaagaacga tctgaaggaa aaatttggca aatcaaaacc gaaaccttct      360

ggaaaagttc tccaattgaa cacttcaaat gacggatcca aatatcaaaa ggctctacaa      420

aaggagttgg cagatctttc cttcaaggag aaattcagcg tagctactga gatcaacgat      480

gatctgagtg agttactcgg cgagaacatt ttcgtttcag actctgtttc aagagatgat      540

gcgaccgaag atattgacgc gttgttgaag gacagctcag ctaaaaagcc cgaaacaata      600

gagagacaaa gcgttgcgcc gacttctcaa aagcttaatt ccatcgatcc cgaggccgat      660

aaatttctcg atgatctact cggctaaatc tcgtacctat tctgctcttt tcgtgtcgtc      720

ttccggttca cccctatctg cattcttatc cataactaat ttatttcatg tatattgcca      780

attacaattg cgcgcaccag cctcgcgttt cattccacag ctgtgcaacc attagggaaa      840

cgtttttcca tcgcgctttc ctcctaatcc tactgaaaaa ctaaaaaaaa acaagttgct      900

tcagtacttt ttctcttttg tggacgtgtt ctaatactta tcatcaaagc aagacatcgg      960

ccggccgc                                                               968


<210>  2
<211>  1135
<212>  DNA
<213>  Artificial

<220>
<223>  Pichia pastoris VRG4 3' knock-out region

<400>  2
ccactagtgc tttgaacaag cttccaattg ccctgtctgg tttgctattt ttcaaggctc       60

ctatcaactt ctattctatc agccctatct ttattggttt tgccgctggt ctagtctatg      120

ccattgccaa gcagaagcaa aagaaggaag acgagttgca gttaccaact gacaagagct      180

agattataag gaaaagaaac actctatata tggtttattt attgattttc agactgaagt      240

ccactatacc gactccctga tggatcgaag aagtactata gatatcaatt tcattgcaca      300

gataatcctt tatattatcc aaagtcaaac ctccactgca ctccaaatag aatttcttgt      360

ttgtgttagc ccacttgttc ttcaaattga tggctgcgac tttcagcccc tcaccggtga      420

aattgtccaa catgataata tcggctcctg ctgctatggc ctcattggct tcagcttcgt      480

cttgcacttc cacctcaatc ttagtgctaa atccaatcac tttttgagca ctttcaatgg      540

ccttggtgat cgaaccagtt gaccagatat ggttgtcttt cagcataatc atagaactta      600

gatcataacg gtgactgtcg catccaccaa cgagcattga gtatttttcc aacaatcgta      660

atcctggagt agtctttctg gttcccgcaa tgattccttt gtatccagct tctctagccc      720

tttttatagt aatatagctt tgagtagcga ccccagagca tcttgctaga atattcagcg      780

ataaccgttc agcgaggagg atgtttcgaa cagggccctt aacgagtgca actttcactt      840

taccctcgtc tccaccacaa atgtaatccc cttcctttag aaaccactcg acctccaaac      900

cgcattgttt gtaaacctct tgtgcaaacg gtactccact aatgactccg ttggacttta      960

tccatagagt agcactctgc aggttttcac ccaccacata tcctccgtaa tcaaaagaag     1020

gggtatcctc gtctagccag ctggtgatat ctttcttcca ttttccgtcc acaggtaaaa     1080

gatgggcaaa ttcggggttg gggttggaga aactcatagt cgtctacgtc gaccc          1135


<210>  3
<211>  2990
<212>  DNA
<213>  Pichia pastoris

<400>  3
aaatatgcca gaaggccgat gatagataat tcatacagat atggtttgga gtctacaaac       60

agtatcactg ttcgtcagga tccagaaatc gaacgggaga tggagttgaa aaggcagcaa      120

gaagaacagg aaaagatgga actgactgat atgatcaaga ctgctctacg agaccaggta      180

gagcatatgc ctgctgccaa aacgatcgat gttaacaaaa tgacgacaga agatttgcta      240

aactggcacc tgggagatca gtccacaaaa aacagttcta tccatcgtga atttgatcca      300

tcagagcaag aagagtttaa taggctagca caaaagattg ccaaagttaa gataaagaac      360

gatctgaagg aaaaatttgg caaatcaaaa ccgaaacctt ctggaaaagt tctccaattg      420

aacacttcaa atgacggatc caaatatcaa aaggctctac aaaaggagtt ggcagatctt      480

tccttcaagg agaaattcag cgtagctact gagatcaacg atgatctgag tgagttactc      540

ggcgagaaca ttttcgtttc agactctgtt tcaagagatg atgcgaccga agatattgac      600

gcgttgttga aggacagctc agctaaaaag cccgaaacaa tagagagaca aagcgttgcg      660

ccgacttctc aaaagcttaa ttccatcgat cccgaggccg ataaatttct cgatgatcta      720

ctcggctaaa tctcgtacct attctgctct tttcgtgtcg tcttccggtt cacccctatc      780

tgcattctta tccataacta atttatttca tgtatattgc caattacaat tgcgcgcacc      840

agcctcgcgt ttcattccac agctgtgcaa ccattaggga aacgtttttc catcgcgctt      900

tcctcctaat cctactgaaa aactaaaaaa aaacaagttg cttcagtact ttttctcttt      960

tgtggacgtg ttctaatact tatcatcaaa gcaagacatc atggctgaca aaggatcggt     1020

agcggctaaa tcgcttacca actctgcacc cttatccatc ttttcttact gtgctgcatc     1080

aattctgatg acagttacca ataagtatgc cgtgtccggt gtcgatttca actttaactt     1140

ctttttgctt gccgttcagg gaatcgtttg tattaccttg attagctcgt tgaagcaatt     1200

gaatgttatc acctttagag agttcaacaa ggttgaagca aagaaatggt tcccaatcgc     1260

cgtgctgtta gttgtcatga tttatacctc ctccaaggct ctacagtatc tgagcattcc     1320

aatttacacg atattcaaaa acttgaccat tatccttatt gcttatggtg aagtcatctg     1380

gttcggaggc cgtgtgacca acttggctct gggctcgttt gtattgatgg tgctctcctc     1440

tgcagtggct tcttatggtg attctaatgt tgacactggt aaactcaatt ttaacattgg     1500

ctatttctgg atgttcacca actgtttctc ctctgccgca tttgtgttgt tcatgagaaa     1560

gagaataaag ttgaccaact tcaaagactt tgacaccatg tattacaaca accttctctc     1620

cattccaatt ttgctctttg catctttgac tactgaagac tggtccgcta aaaacatagc     1680

tcagaacttc cctgaagaca ccaaatacgc tgtcatcgct tccatgatta tttcaggaat     1740

gtctgccgtg ggtatctcat acacatctgc atggtgtgtc cgtgtgacat cttccacgac     1800

atactcgatg gttggtgctt tgaacaagct tccaattgcc ctgtctggtt tgctattttt     1860

caaggctcct atcaacttct attctatcag ctctatcttt attggttttg ccgctggtct     1920

agtctatgcc attgccaagc agaagcaaaa gaaggaagac gagttgcagt taccaactga     1980

caagagctag attataagga aaagaaacac tctatatatg gtttatttat tgattttcag     2040

actgaagtcc actataccga ctccctgatg gatcgaagaa gtactataga tatcaatttc     2100

attgcacaga taatccttta tattatccaa agtcaaacct ccactgcact ccaaatagaa     2160

tttcttgttt gtgttagccc acttgttctt caaattgatg gctgcgactt tcagcccctc     2220

accggtgaaa ttgtccaaca tgataatatc ggctcctgct gctatggcct cattggcttc     2280

agcttcgtct tgcacttcca cctcaatctt agtgctaaat ccaatcactt tttgagcact     2340

ttcaatggcc ttggtgatcg aaccagttga ccagatatgg ttgtctttca gcataatcat     2400

agaacttaga tcataacggt gactgtcgca tccaccaacg agcattgagt atttttccaa     2460

caatcgtaat cctggagtag tctttctggt tcccgcaatg attcctttgt atccagcttc     2520

tctagccctt tttatagtaa tatagctttg agtagcgacc ccagagcatc ttgctagaat     2580

attcagcgat aaccgttcag cgaggaggat gtttcgaaca gggcccttaa cgagtgcaac     2640

tttcacttta ccctcgtctc caccacaaat gtaatcccct tcctttagaa accactcgac     2700

ctccaaaccg cattgtttgt aaacctcttg tgcaaacggt actccactaa tgactccgtt     2760

ggactttatc catagagtag cactctgcag gttttcaccc accacatatc ctccgtaatc     2820

aaaagaaggg gtatcctcgt ctagccagct ggtgatatct ttcttccatt ttccgtccac     2880

aggtaaaaga tgggcaaatt cggggttggg gttggagaaa ctcatagtcg tctacaaatg     2940

tgaaggaaat ggatgatatt gttaggccat tctccgcgag tcattccggg                2990


<210>  4
<211>  484
<212>  PRT
<213>  Artificial

<220>
<223>  HSA-TNFR-Fc double mutein ORF

<400>  4

Met Lys Trp Val Thr Phe Ile Ser Leu Leu Phe Leu Phe Ser Ser Ala 
1               5                   10                  15      


Tyr Ser Leu Pro Ala Gln Val Ala Phe Thr Pro Tyr Ala Pro Glu Pro 
            20                  25                  30          


Gly Ser Thr Cys Arg Leu Arg Glu Tyr Tyr Asp Gln Thr Ala Gln Met 
        35                  40                  45              


Cys Cys Ser Lys Cys Ser Pro Gly Gln His Ala Lys Val Phe Cys Thr 
    50                  55                  60                  


Lys Thr Ser Asp Thr Val Cys Asp Ser Cys Glu Asp Ser Thr Tyr Thr 
65                  70                  75                  80  


Gln Leu Trp Asn Trp Val Pro Glu Cys Leu Ser Cys Gly Ser Arg Cys 
                85                  90                  95      


Ser Ser Asp Gln Val Glu Thr Gln Ala Cys Thr Arg Glu Gln Asn Arg 
            100                 105                 110         


Ile Cys Thr Cys Arg Pro Gly Trp Tyr Cys Ala Leu Ser Lys Gln Glu 
        115                 120                 125             


Gly Cys Arg Leu Cys Ala Pro Leu Arg Lys Cys Arg Pro Gly Phe Gly 
    130                 135                 140                 


Val Ala Arg Pro Gly Thr Glu Thr Ser Asp Val Val Cys Lys Pro Cys 
145                 150                 155                 160 


Ala Pro Gly Thr Phe Ser Asn Thr Thr Ser Ser Thr Asp Ile Cys Arg 
                165                 170                 175     


Pro His Gln Ile Cys Asn Val Val Ala Ile Pro Gly Asn Ala Ser Met 
            180                 185                 190         


Asp Ala Val Cys Thr Ser Thr Ser Pro Thr Arg Ser Met Ala Pro Gly 
        195                 200                 205             


Ala Val His Leu Pro Gln Pro Val Ser Thr Arg Ser Gln His Thr Gln 
    210                 215                 220                 


Pro Thr Pro Glu Pro Ser Thr Ala Pro Ser Thr Ser Phe Leu Leu Pro 
225                 230                 235                 240 


Met Gly Pro Ser Pro Pro Ala Glu Gly Ser Thr Gly Asp Glu Pro Lys 
                245                 250                 255     


Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
            260                 265                 270         


Leu Gly Gly Pro Ser Val Phe Leu Ala Pro Pro Lys Pro Lys Asp Thr 
        275                 280                 285             


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Ala Asp Val 
    290                 295                 300                 


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
305                 310                 315                 320 


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
                325                 330                 335     


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
            340                 345                 350         


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
        355                 360                 365             


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
    370                 375                 380                 


Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln 
385                 390                 395                 400 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
                405                 410                 415     


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
            420                 425                 430         


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
        435                 440                 445             


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
    450                 455                 460                 


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
465                 470                 475                 480 


Leu Ser Pro Gly 
                


<210>  5
<211>  1455
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence encoding HSA-TNFR-Fc double mutein ORF

<400>  5
atgaagtggg ttacctttat ctctttgttg tttcttttct cttctgctta ctctctgcca       60

gctcaagttg cttttactcc atacgctcca gaaccaggtt ctacttgtag attgagagag      120

tactacgacc aaactgctca gatgtgttgt tccaagtgtt ctccaggtca acacgctaag      180

gttttctgta ctaagacttc cgacactgtt tgtgactctt gtgaggactc cacttacact      240

caattgtgga actgggttcc agaatgtttg tcctgtggtt ccagatgttc ttccgaccaa      300

gttgagactc aggcttgtac tagagagcag aacagaatct gtacttgtag acctggttgg      360

tactgtgctt tgtccaagca agagggttgt agattgtgtg ctccattgag aaagtgtaga      420

ccaggtttcg gtgttgctag accaggtaca gaaacttccg acgttgtttg taagccatgt      480

gctccaggaa ctttctccaa cactacttcc tccactgaca tctgtagacc acaccaaatc      540

tgtaacgttg ttgctatccc aggtaacgct tctatggacg ctgtttgtac ttctacttcc      600

ccaactagat ccatggctcc aggtgctgtt catttgccac agccagtttc cactagatcc      660

caacacactc aaccaactcc agaaccatct actgctccat ccacttcctt tttgttgcca      720

atgggaccat ctccacctgc tgaaggttct actggtgacg agccaaagtc ctgtgacaag      780

acacatactt gtccaccatg tccagctcca gaattgttgg gtggtccatc cgttttcttg      840

gccccaccaa agccaaagga cactttgatg atctccagaa ctccagaggt tacatgtgtt      900

gttgctgacg tttctcacga ggacccagag gttaagttca actggtacgt tgacggtgtt      960

gaagttcaca acgctaagac taagccaaga gaagagcagt acaactccac ttacagagtt     1020

gtttccgttt tgactgtttt gcaccaggat tggttgaacg gtaaagaata caagtgtaag     1080

gtttccaaca aggctttgcc agctccaatc gaaaagacaa tctccaaggc taagggtcaa     1140

ccaagagagc cacaggttta cactttgcca ccatccagag aagagatgac taagaaccag     1200

gtttccttga cttgtttggt taaaggattc tacccatccg acattgctgt tgaatgggaa     1260

tctaacggtc aaccagagaa caactacaag actactccac cagttttgga ttctgacggt     1320

tccttcttct tgtactccaa gttgactgtt gacaagtcca gatggcaaca gggtaacgtt     1380

ttctcctgtt ccgttatgca tgaggctttg cacaaccact acactcaaaa gtccttgtct     1440

ttgtccccag gttag                                                      1455


<210>  6
<211>  280
<212>  PRT
<213>  Artificial

<220>
<223>  alpah--mating factor prepro EPO ORF

<400>  6

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
            20                  25                  30          


Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe 
        35                  40                  45              


Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
    50                  55                  60                  


Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
65                  70                  75                  80  


Ser Leu Glu Lys Arg Glu Ala Glu Ala Glu Phe Ala Pro Pro Arg Leu 
                85                  90                  95      


Ile Cys Asp Ser Arg Val Leu Glu Arg Tyr Ile Leu Glu Ala Lys Glu 
            100                 105                 110         


Ala Glu Asn Val Thr Met Gly Cys Ala Glu Gly Pro Arg Leu Ser Glu 
        115                 120                 125             


Asn Ile Thr Val Pro Asp Thr Lys Val Asn Phe Tyr Ala Trp Lys Arg 
    130                 135                 140                 


Met Lys Val Glu Glu Gln Ala Val Glu Val Trp Gln Gly Leu Ser Leu 
145                 150                 155                 160 


Leu Ser Glu Ala Ile Leu Gln Ala Gln Ala Leu Gln Ala Asn Ser Ser 
                165                 170                 175     


Gln Pro Pro Glu Ser Leu Gln Leu His Ile Asp Lys Ala Ile Ser Gly 
            180                 185                 190         


Leu Arg Ser Leu Thr Ser Leu Leu Arg Val Leu Gly Ala Gln Lys Glu 
        195                 200                 205             


Leu Met Ser Pro Pro Asp Ala Thr Gln Ala Ala Pro Leu Arg Thr Leu 
    210                 215                 220                 


Thr Ala Asp Thr Phe Cys Lys Leu Phe Arg Val Tyr Ser Asn Phe Leu 
225                 230                 235                 240 


Arg Gly Lys Leu Lys Leu Tyr Thr Gly Glu Ala Cys Arg Arg Gly Asp 
                245                 250                 255     


Arg Gly Leu Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Asn Ser Ala 
            260                 265                 270         


Val Asp His His His His His His 
        275                 280 


<210>  7
<211>  843
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence encoding alpha-mating factor prepro EPO ORF

<400>  7
atgagatttc cttcaatttt tactgctgtt ttattcgcag catcctccgc attagctgct       60

ccagtcaaca ctacaacaga agatgaaacg gcacaaattc cggctgaagc tgtcatcggt      120

tactcagatt tagaagggga tttcgatgtt gctgttttgc cattttccaa cagcacaaat      180

aacgggttat tgtttataaa tactactatt gccagcattg ctgctaaaga agaaggggta      240

tctctggaga aaagagaggc tgaagctgag ttcgctcccc cacgcctcat ttgcgacagt      300

cgcgttctgg agaggtacat cttggaggcc aaggaggcag aaaatgtcac aatgggctgt      360

gcagaaggtc ccagactgag tgagaatatt accgtcccag ataccaaagt caacttctac      420

gcttggaaaa gaatgaaggt ggaagaacag gctgtagaag tttggcaagg cctgtctctg      480

ctctcagaag ccatcctgca ggcccaggct ctgcaggcca attcctccca gccaccagag      540

agtcttcagc ttcatataga caaagccatc agtgggctac gtagcctcac ttcactgctt      600

cgggtgctgg gagctcagaa ggaattgatg tcgcctccag acgccaccca agccgctcca      660

ctccgaacac tcacagcgga tactttctgc aagctcttcc gggtctactc caacttcctc      720

cgggggaaac tgaagctgta cacgggggag gcctgcagga gaggggacag gggtctggaa      780

caaaaactca tctcagaaga ggatctgaat agcgccgtcg accatcatca tcatcatcat      840

tga                                                                    843


<210>  8
<211>  2250
<212>  DNA
<213>  Pichia pastoris

<400>  8
caatacaagg ttgtcttgga gtttacattg accagatgat ttggcttttt ctctgttcaa       60

ttcacatttt tcagcgagaa tcggattgac ggagaaatgg cggggtgtgg ggtggataga      120

tggcagaaat gctcgcaatc accgcgaaag aaagacttta tggaatagaa ctactgggtg      180

gtgtaaggat tacatagcta gtccaatgga gtccgttgga aaggtaagaa gaagctaaaa      240

ccggctaagt aactagggaa gaatgatcag actttgattt gatgaggtct gaaaatactc      300

tgctgctttt tcagttgctt tttccctgca acctatcatt ttccttttca taagcctgcc      360

ttttctgttt tcacttatat gagttccgcc gagacttccc caaattctct cctggaacat      420

tctctatcgc tctccttcca agttgcgccc cctggcactg cctagtaata ttaccacgcg      480

acttatattc agttccacaa tttccagtgt tcgtagcaaa tatcatcagc catggcgaag      540

gcagatggca gtttgctcta ctataatcct cacaatccac ccagaaggta ttacttctac      600

atggctatat tcgccgtttc tgtcatttgc gttttgtacg gaccctcaca acaattatca      660

tctccaaaaa tagactatga tccattgacg ctccgatcac ttgatttgaa gactttggaa      720

gctccttcac agttgagtcc aggcaccgta gaagataatc ttcgaagaca attggagttt      780

cattttcctt accgcagtta cgaacctttt ccccaacata tttggcaaac gtggaaagtt      840

tctccctctg atagttcctt tccgaaaaac ttcaaagact taggtgaaag ttggctgcaa      900

aggtccccaa attatgatca ttttgtgata cccgatgatg cagcatggga acttattcac      960

catgaatacg aacgtgtacc agaagtcttg gaagctttcc acctgctacc agagcccatt     1020

ctaaaggccg attttttcag gtatttgatt ctttttgccc gtggaggact gtatgctgac     1080

atggacacta tgttattaaa accaatagaa tcgtggctga ctttcaatga aactattggt     1140

ggagtaaaaa acaatgctgg gttggtcatt ggtattgagg ctgatcctga tagacctgat     1200

tggcacgact ggtatgctag aaggatacaa ttttgccaat gggcaattca gtccaaacga     1260

ggacacccag cactgcgtga actgattgta agagttgtca gcacgacttt acggaaagag     1320

aaaagcggtt acttgaacat ggtggaagga aaggatcgtg gaagtgatgt gatggactgg     1380

acgggtccag gaatatttac agacactcta tttgattata tgactaatgt caatacaaca     1440

ggccactcag gccaaggaat tggagctggc tcagcgtatt acaatgcctt atcgttggaa     1500

gaacgtgatg ccctctctgc ccgcccgaac ggagagatgt taaaagagaa agtcccaggt     1560

aaatatgcac agcaggttgt tttatgggaa caatttacca acctgcgctc ccccaaatta     1620

atcgacgata ttcttattct tccgatcacc agcttcagtc cagggattgg ccacagtgga     1680

gctggagatt tgaaccatca ccttgcatat attaggcata catttgaagg aagttggaag     1740

gactaaagaa agctagagta aaatagatat agcgagatta gagaatgaat accttcttct     1800

aagcgatcgt ccgtcatcat agaatatcat ggactgtata gttttttttt tgtacatata     1860

atgattaaac ggtcatccaa catctcgttg acagatctct cagtacgcga aatccctgac     1920

tatcaaagca agaaccgatg aagaaaaaaa caacagtaac ccaaacacca caacaaacac     1980

tttatcttct cccccccaac accaatcatc aaagagatgt cggaaccaaa caccaagaag     2040

caaaaactaa ccccatataa aaacatcctg gtagataatg ctggtaaccc gctctccttc     2100

catattctgg gctacttcac gaagtctgac cggtctcagt tgatcaacat gatcctcgaa     2160

atgggtggca agatcgttcc agacctgcct cctctggtag atggagtgtt gtttttgaca     2220

ggggattaca agtctattga tgaagatacc                                      2250


<210>  9
<211>  3029
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  9
aggcctcgca acaacctata attgagttaa gtgcctttcc aagctaaaaa gtttgaggtt       60

ataggggctt agcatccaca cgtcacaatc tcgggtatcg agtatagtat gtagaattac      120

ggcaggaggt ttcccaatga acaaaggaca ggggcacggt gagctgtcga aggtatccat      180

tttatcatgt ttcgtttgta caagcacgac atactaagac atttaccgta tgggagttgt      240

tgtcctagcg tagttctcgc tcccccagca aagctcaaaa aagtacgtca tttagaatag      300

tttgtgagca aattaccagt cggtatgcta cgttagaaag gcccacagta ttcttctacc      360

aaaggcgtgc ctttgttgaa ctcgatccat tatgagggct tccattattc cccgcatttt      420

tattactctg aacaggaata aaaagaaaaa acccagttta ggaaattatc cgggggcgaa      480

gaaatacgcg tagcgttaat cgaccccacg tccagggttt ttccatggag gtttctggaa      540

aaactgacga ggaatgtgat tataaatccc tttatgtgat gtctaagact tttaaggtac      600

gcccgatgtt tgcctattac catcatagag acgtttcttt tcgaggaatg cttaaacgac      660

tttgtttgac aaaaatgttg cctaagggct ctatagtaaa ccatttggaa gaaagatttg      720

acgacttttt ttttttggat ttcgatccta taatccttcc tcctgaaaag aaacatataa      780

atagatatgt attattcttc aaaacattct cttgttcttg tgcttttttt ttaccatata      840

tcttactttt ttttttctct cagagaaaca agcaaaacaa aaagcttttc ttttcactaa      900

cgtatatgat gcttttgcaa gctttccttt tccttttggc tggttttgca gccaaaatat      960

ctgcatcaat gacaaacgaa actagcgata gacctttggt ccacttcaca cccaacaagg     1020

gctggatgaa tgacccaaat gggttgtggt acgatgaaaa agatgccaaa tggcatctgt     1080

actttcaata caacccaaat gacaccgtat ggggtacgcc attgttttgg ggccatgcta     1140

cttccgatga tttgactaat tgggaagatc aacccattgc tatcgctccc aagcgtaacg     1200

attcaggtgc tttctctggc tccatggtgg ttgattacaa caacacgagt gggtttttca     1260

atgatactat tgatccaaga caaagatgcg ttgcgatttg gacttataac actcctgaaa     1320

gtgaagagca atacattagc tattctcttg atggtggtta cacttttact gaataccaaa     1380

agaaccctgt tttagctgcc aactccactc aattcagaga tccaaaggtg ttctggtatg     1440

aaccttctca aaaatggatt atgacggctg ccaaatcaca agactacaaa attgaaattt     1500

actcctctga tgacttgaag tcctggaagc tagaatctgc atttgccaat gaaggtttct     1560

taggctacca atacgaatgt ccaggtttga ttgaagtccc aactgagcaa gatccttcca     1620

aatcttattg ggtcatgttt atttctatca acccaggtgc acctgctggc ggttccttca     1680

accaatattt tgttggatcc ttcaatggta ctcattttga agcgtttgac aatcaatcta     1740

gagtggtaga ttttggtaag gactactatg ccttgcaaac tttcttcaac actgacccaa     1800

cctacggttc agcattaggt attgcctggg cttcaaactg ggagtacagt gcctttgtcc     1860

caactaaccc atggagatca tccatgtctt tggtccgcaa gttttctttg aacactgaat     1920

atcaagctaa tccagagact gaattgatca atttgaaagc cgaaccaata ttgaacatta     1980

gtaatgctgg tccctggtct cgttttgcta ctaacacaac tctaactaag gccaattctt     2040

acaatgtcga tttgagcaac tcgactggta ccctagagtt tgagttggtt tacgctgtta     2100

acaccacaca aaccatatcc aaatccgtct ttgccgactt atcactttgg ttcaagggtt     2160

tagaagatcc tgaagaatat ttgagaatgg gttttgaagt cagtgcttct tccttctttt     2220

tggaccgtgg taactctaag gtcaagtttg tcaaggagaa cccatatttc acaaacagaa     2280

tgtctgtcaa caaccaacca ttcaagtctg agaacgacct aagttactat aaagtgtacg     2340

gcctactgga tcaaaacatc ttggaattgt acttcaacga tggagatgtg gtttctacaa     2400

atacctactt catgaccacc ggtaacgctc taggatctgt gaacatgacc actggtgtcg     2460

ataatttgtt ctacattgac aagttccaag taagggaagt aaaatagagg ttataaaact     2520

tattgtcttt tttatttttt tcaaaagcca ttctaaaggg ctttagctaa cgagtgacga     2580

atgtaaaact ttatgatttc aaagaatacc tccaaaccat tgaaaatgta tttttatttt     2640

tattttctcc cgaccccagt tacctggaat ttgttcttta tgtactttat ataagtataa     2700

ttctcttaaa aatttttact actttgcaat agacatcatt ttttcacgta ataaacccac     2760

aatcgtaatg tagttgcctt acactactag gatggacctt tttgccttta tctgttttgt     2820

tactgacaca atgaaaccgg gtaaagtatt agttatgtga aaatttaaaa gcattaagta     2880

gaagtatacc atattgtaaa aaaaaaaagc gttgtcttct acgtaaaagt gttctcaaaa     2940

agaagtagtg agggaaatgg ataccaagct atctgtaaca ggagctaaaa aatctcaggg     3000

aaaagcttct ggtttgggaa acggtcgac                                       3029


<210>  10
<211>  898
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the 5'-Region used for knock out of PpURA5

<400>  10
atcggccttt gttgatgcaa gttttacgtg gatcatggac taaggagttt tatttggacc       60

aagttcatcg tcctagacat tacggaaagg gttctgctcc tctttttgga aactttttgg      120

aacctctgag tatgacagct tggtggattg tacccatggt atggcttcct gtgaatttct      180

attttttcta cattggattc accaatcaaa acaaattagt cgccatggct ttttggcttt      240

tgggtctatt tgtttggacc ttcttggaat atgctttgca tagatttttg ttccacttgg      300

actactatct tccagagaat caaattgcat ttaccattca tttcttattg catgggatac      360

accactattt accaatggat aaatacagat tggtgatgcc acctacactt ttcattgtac      420

tttgctaccc aatcaagacg ctcgtctttt ctgttctacc atattacatg gcttgttctg      480

gatttgcagg tggattcctg ggctatatca tgtatgatgt cactcattac gttctgcatc      540

actccaagct gcctcgttat ttccaagagt tgaagaaata tcatttggaa catcactaca      600

agaattacga gttaggcttt ggtgtcactt ccaaattctg ggacaaagtc tttgggactt      660

atctgggtcc agacgatgtg tatcaaaaga caaattagag tatttataaa gttatgtaag      720

caaatagggg ctaataggga aagaaaaatt ttggttcttt atcagagctg gctcgcgcgc      780

agtgtttttc gtgctccttt gtaatagtca tttttgacta ctgttcagat tgaaatcaca      840

ttgaagatgt cactcgaggg gtaccaaaaa aggtttttgg atgctgcagt ggcttcgc        898


<210>  11
<211>  1060
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the 3'-Region used for knock out of PpURA5

<400>  11
ggtcttttca acaaagctcc attagtgagt cagctggctg aatcttatgc acaggccatc       60

attaacagca acctggagat agacgttgta tttggaccag cttataaagg tattcctttg      120

gctgctatta ccgtgttgaa gttgtacgag ctcggcggca aaaaatacga aaatgtcgga      180

tatgcgttca atagaaaaga aaagaaagac cacggagaag gtggaagcat cgttggagaa      240

agtctaaaga ataaaagagt actgattatc gatgatgtga tgactgcagg tactgctatc      300

aacgaagcat ttgctataat tggagctgaa ggtgggagag ttgaaggtag tattattgcc      360

ctagatagaa tggagactac aggagatgac tcaaatacca gtgctaccca ggctgttagt      420

cagagatatg gtacccctgt cttgagtata gtgacattgg accatattgt ggcccatttg      480

ggcgaaactt tcacagcaga cgagaaatct caaatggaaa cgtatagaaa aaagtatttg      540

cccaaataag tatgaatctg cttcgaatga atgaattaat ccaattatct tctcaccatt      600

attttcttct gtttcggagc tttgggcacg gcggcgggtg gtgcgggctc aggttccctt      660

tcataaacag atttagtact tggatgctta atagtgaatg gcgaatgcaa aggaacaatt      720

tcgttcatct ttaacccttt cactcggggt acacgttctg gaatgtaccc gccctgttgc      780

aactcaggtg gaccgggcaa ttcttgaact ttctgtaacg ttgttggatg ttcaaccaga      840

aattgtccta ccaactgtat tagtttcctt ttggtcttat attgttcatc gagatacttc      900

ccactctcct tgatagccac tctcactctt cctggattac caaaatcttg aggatgagtc      960

ttttcaggct ccaggatgca aggtatatcc aagtacctgc aagcatctaa tattgtcttt     1020

gccagggggt tctccacacc atactccttt tggcgcatgc                           1060


<210>  12
<211>  957
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the PpURA5 auxotrophic marker

<400>  12
tctagaggga cttatctggg tccagacgat gtgtatcaaa agacaaatta gagtatttat       60

aaagttatgt aagcaaatag gggctaatag ggaaagaaaa attttggttc tttatcagag      120

ctggctcgcg cgcagtgttt ttcgtgctcc tttgtaatag tcatttttga ctactgttca      180

gattgaaatc acattgaaga tgtcactgga ggggtaccaa aaaaggtttt tggatgctgc      240

agtggcttcg caggccttga agtttggaac tttcaccttg aaaagtggaa gacagtctcc      300

atacttcttt aacatgggtc ttttcaacaa agctccatta gtgagtcagc tggctgaatc      360

ttatgctcag gccatcatta acagcaacct ggagatagac gttgtatttg gaccagctta      420

taaaggtatt cctttggctg ctattaccgt gttgaagttg tacgagctgg gcggcaaaaa      480

atacgaaaat gtcggatatg cgttcaatag aaaagaaaag aaagaccacg gagaaggtgg      540

aagcatcgtt ggagaaagtc taaagaataa aagagtactg attatcgatg atgtgatgac      600

tgcaggtact gctatcaacg aagcatttgc tataattgga gctgaaggtg ggagagttga      660

aggttgtatt attgccctag atagaatgga gactacagga gatgactcaa ataccagtgc      720

tacccaggct gttagtcaga gatatggtac ccctgtcttg agtatagtga cattggacca      780

tattgtggcc catttgggcg aaactttcac agcagacgag aaatctcaaa tggaaacgta      840

tagaaaaaag tatttgccca aataagtatg aatctgcttc gaatgaatga attaatccaa      900

ttatcttctc accattattt tcttctgttt cggagctttg ggcacggcgg cggatcc         957


<210>  13
<211>  709
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the part of the Ec lacZ gene that was used to 
       construct the PpURA5 blaster (recyclable auxotrophic marker)

<400>  13
cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag tgcctctgga       60

tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc cggagagcgc      120

cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc      180

cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg tgacgctccc      240

cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt gcatcgagct      300

gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg      360

cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac cgctggataa      420

cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg aacgctggaa      480

ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag atacacttgc      540

tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa ccttatttat      600

cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg ttgatgttga      660

agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccag                  709


<210>  14
<211>  2875
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the 5'-Region used for knock out of PpOCH1

<400>  14
aaaacctttt ttcctattca aacacaaggc attgcttcaa cacgtgtgcg tatccttaac       60

acagatactc catacttcta ataatgtgat agacgaatac aaagatgttc actctgtgtt      120

gtgtctacaa gcatttctta ttctgattgg ggatattcta gttacagcac taaacaactg      180

gcgatacaaa cttaaattaa ataatccgaa tctagaaaat gaacttttgg atggtccgcc      240

tgttggttgg ataaatcaat accgattaaa tggattctat tccaatgaga gagtaatcca      300

agacactctg atgtcaataa tcatttgctt gcaacaacaa acccgtcatc taatcaaagg      360

gtttgatgag gcttaccttc aattgcagat aaactcattg ctgtccactg ctgtattatg      420

tgagaatatg ggtgatgaat ctggtcttct ccactcagct aacatggctg tttgggcaaa      480

ggtggtacaa ttatacggag atcaggcaat agtgaaattg ttgaatatgg ctactggacg      540

atgcttcaag gatgtacgtc tagtaggagc cgtgggaaga ttgctggcag aaccagttgg      600

cacgtcgcaa caatccccaa gaaatgaaat aagtgaaaac gtaacgtcaa agacagcaat      660

ggagtcaata ttgataacac cactggcaga gcggttcgta cgtcgttttg gagccgatat      720

gaggctcagc gtgctaacag cacgattgac aagaagactc tcgagtgaca gtaggttgag      780

taaagtattc gcttagattc ccaaccttcg ttttattctt tcgtagacaa agaagctgca      840

tgcgaacata gggacaactt ttataaatcc aattgtcaaa ccaacgtaaa accctctggc      900

accattttca acatatattt gtgaagcagt acgcaatatc gataaatact caccgttgtt      960

tgtaacagcc ccaacttgca tacgccttct aatgacctca aatggataag ccgcagcttg     1020

tgctaacata ccagcagcac cgcccgcggt cagctgcgcc cacacatata aaggcaatct     1080

acgatcatgg gaggaattag ttttgaccgt caggtcttca agagttttga actcttcttc     1140

ttgaactgtg taacctttta aatgacggga tctaaatacg tcatggatga gatcatgtgt     1200

gtaaaaactg actccagcat atggaatcat tccaaagatt gtaggagcga acccacgata     1260

aaagtttccc aaccttgcca aagtgtctaa tgctgtgact tgaaatctgg gttcctcgtt     1320

gaagaccctg cgtactatgc ccaaaaactt tcctccacga gccctattaa cttctctatg     1380

agtttcaaat gccaaacgga cacggattag gtccaatggg taagtgaaaa acacagagca     1440

aaccccagct aatgagccgg ccagtaaccg tcttggagct gtttcataag agtcattagg     1500

gatcaataac gttctaatct gttcataaca tacaaatttt atggctgcat agggaaaaat     1560

tctcaacagg gtagccgaat gaccctgata tagacctgcg acaccatcat acccatagat     1620

ctgcctgaca gccttaaaga gcccgctaaa agacccggaa aaccgagaga actctggatt     1680

agcagtctga aaaagaatct tcactctgtc tagtggagca attaatgtct tagcggcact     1740

tcctgctact ccgccagcta ctcctgaata gatcacatac tgcaaagact gcttgtcgat     1800

gaccttgggg ttatttagct tcaagggcaa tttttgggac attttggaca caggagactc     1860

agaaacagac acagagcgtt ctgagtcctg gtgctcctga cgtaggccta gaacaggaat     1920

tattggcttt atttgtttgt ccatttcata ggcttggggt aatagataga tgacagagaa     1980

atagagaaga cctaatattt tttgttcatg gcaaatcgcg ggttcgcggt cgggtcacac     2040

acggagaagt aatgagaaga gctggtaatc tggggtaaaa gggttcaaaa gaaggtcgcc     2100

tggtagggat gcaatacaag gttgtcttgg agtttacatt gaccagatga tttggctttt     2160

tctctgttca attcacattt ttcagcgaga atcggattga cggagaaatg gcggggtgtg     2220

gggtggatag atggcagaaa tgctcgcaat caccgcgaaa gaaagacttt atggaataga     2280

actactgggt ggtgtaagga ttacatagct agtccaatgg agtccgttgg aaaggtaaga     2340

agaagctaaa accggctaag taactaggga agaatgatca gactttgatt tgatgaggtc     2400

tgaaaatact ctgctgcttt ttcagttgct ttttccctgc aacctatcat tttccttttc     2460

ataagcctgc cttttctgtt ttcacttata tgagttccgc cgagacttcc ccaaattctc     2520

tcctggaaca ttctctatcg ctctccttcc aagttgcgcc ccctggcact gcctagtaat     2580

attaccacgc gacttatatt cagttccaca atttccagtg ttcgtagcaa atatcatcag     2640

ccatggcgaa ggcagatggc agtttgctct actataatcc tcacaatcca cccagaaggt     2700

attacttcta catggctata ttcgccgttt ctgtcatttg cgttttgtac ggaccctcac     2760

aacaattatc atctccaaaa atagactatg atccattgac gctccgatca cttgatttga     2820

agactttgga agctccttca cagttgagtc caggcaccgt agaagataat cttcg          2875


<210>  15
<211>  997
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the 3'-Region used for knock out of PpOCH1

<400>  15
aaagctagag taaaatagat atagcgagat tagagaatga ataccttctt ctaagcgatc       60

gtccgtcatc atagaatatc atggactgta tagttttttt tttgtacata taatgattaa      120

acggtcatcc aacatctcgt tgacagatct ctcagtacgc gaaatccctg actatcaaag      180

caagaaccga tgaagaaaaa aacaacagta acccaaacac cacaacaaac actttatctt      240

ctccccccca acaccaatca tcaaagagat gtcggaacca aacaccaaga agcaaaaact      300

aaccccatat aaaaacatcc tggtagataa tgctggtaac ccgctctcct tccatattct      360

gggctacttc acgaagtctg accggtctca gttgatcaac atgatcctcg aaatgggtgg      420

caagatcgtt ccagacctgc ctcctctggt agatggagtg ttgtttttga caggggatta      480

caagtctatt gatgaagata ccctaaagca actgggggac gttccaatat acagagactc      540

cttcatctac cagtgttttg tgcacaagac atctcttccc attgacactt tccgaattga      600

caagaacgtc gacttggctc aagatttgat caatagggcc cttcaagagt ctgtggatca      660

tgtcacttct gccagcacag ctgcagctgc tgctgttgtt gtcgctacca acggcctgtc      720

ttctaaacca gacgctcgta ctagcaaaat acagttcact cccgaagaag atcgttttat      780

tcttgacttt gttaggagaa atcctaaacg aagaaacaca catcaactgt acactgagct      840

cgctcagcac atgaaaaacc atacgaatca ttctatccgc cacagatttc gtcgtaatct      900

ttccgctcaa cttgattggg tttatgatat cgatccattg accaaccaac ctcgaaaaga      960

tgaaaacggg aactacatca aggtacaagg ccttcca                               997


<210>  16
<211>  2159
<212>  DNA
<213>  Artificial

<220>
<223>  K. lactis UDP-GlcNAc transporter gene (KIMNN2-2)

<400>  16
aaacgtaacg cctggcactc tattttctca aacttctggg acggaagagc taaatattgt       60

gttgcttgaa caaacccaaa aaaacaaaaa aatgaacaaa ctaaaactac acctaaataa      120

accgtgtgta aaacgtagta ccatattact agaaaagatc acaagtgtat cacacatgtg      180

catctcatat tacatctttt atccaatcca ttctctctat cccgtctgtt cctgtcagat      240

tctttttcca taaaaagaag aagaccccga atctcaccgg tacaatgcaa aactgctgaa      300

aaaaaaagaa agttcactgg atacgggaac agtgccagta ggcttcacca catggacaaa      360

acaattgacg ataaaataag caggtgagct tctttttcaa gtcacgatcc ctttatgtct      420

cagaaacaat atatacaagc taaacccttt tgaaccagtt ctctcttcat agttatgttc      480

acataaattg cgggaacaag actccgctgg ctgtcaggta cacgttgtaa cgttttcgtc      540

cgcccaatta ttagcacaac attggcaaaa agaaaaactg ctcgttttct ctacaggtaa      600

attacaattt ttttcagtaa ttttcgctga aaaatttaaa gggcaggaaa aaaagacgat      660

ctcgactttg catagatgca agaactgtgg tcaaaacttg aaatagtaat tttgctgtgc      720

gtgaactaat aaatatatat atatatatat atatatattt gtgtattttg tatatgtaat      780

tgtgcacgtc ttggctattg gatataagat tttcgcgggt tgatgacata gagcgtgtac      840

tactgtaata gttgtatatt caaaagctgc tgcgtggaga aagactaaaa tagataaaaa      900

gcacacattt tgacttcggt accgtcaact tagtgggaca gtcttttata tttggtgtaa      960

gctcatttct ggtactattc gaaacagaac agtgttttct gtattaccgt ccaatcgttt     1020

gtcatgagtt ttgtattgat tttgtcgtta gtgttcggag gatgttgttc caatgtgatt     1080

agtttcgagc acatggtgca aggcagcaat ataaatttgg gaaatattgt tacattcact     1140

caattcgtgt ctgtgacgct aattcagttg cccaatgctt tggacttctc tcactttccg     1200

tttaggttgc gacctagaca cattcctctt aagatccata tgttagctgt gtttttgttc     1260

tttaccagtt cagtcgccaa taacagtgtg tttaaatttg acatttccgt tccgattcat     1320

attatcatta gattttcagg taccactttg acgatgataa taggttgggc tgtttgtaat     1380

aagaggtact ccaaacttca ggtgcaatct gccatcatta tgacgcttgg tgcgattgtc     1440

gcatcattat accgtgacaa agaattttca atggacagtt taaagttgaa tacggattca     1500

gtgggtatga cccaaaaatc tatgtttggt atctttgttg tgctagtggc cactgccttg     1560

atgtcattgt tgtcgttgct caacgaatgg acgtataaca agtacgggaa acattggaaa     1620

gaaactttgt tctattcgca tttcttggct ctaccgttgt ttatgttggg gtacacaagg     1680

ctcagagacg aattcagaga cctcttaatt tcctcagact caatggatat tcctattgtt     1740

aaattaccaa ttgctacgaa acttttcatg ctaatagcaa ataacgtgac ccagttcatt     1800

tgtatcaaag gtgttaacat gctagctagt aacacggatg ctttgacact ttctgtcgtg     1860

cttctagtgc gtaaatttgt tagtctttta ctcagtgtct acatctacaa gaacgtccta     1920

tccgtgactg catacctagg gaccatcacc gtgttcctgg gagctggttt gtattcatat     1980

ggttcggtca aaactgcact gcctcgctga aacaatccac gtctgtatga tactcgtttc     2040

agaatttttt tgattttctg ccggatatgg tttctcatct ttacaatcgc attcttaatt     2100

ataccagaac gtaattcaat gatcccagtg actcgtaact cttatatgtc aatttaagc      2159


<210>  17
<211>  870
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the 5'-Region used for knock out of PpBMT2

<400>  17
ggccgagcgg gcctagattt tcactacaaa tttcaaaact acgcggattt attgtctcag       60

agagcaattt ggcatttctg agcgtagcag gaggcttcat aagattgtat aggaccgtac      120

caacaaattg ccgaggcaca acacggtatg ctgtgcactt atgtggctac ttccctacaa      180

cggaatgaaa ccttcctctt tccgcttaaa cgagaaagtg tgtcgcaatt gaatgcaggt      240

gcctgtgcgc cttggtgtat tgtttttgag ggcccaattt atcaggcgcc ttttttcttg      300

gttgttttcc cttagcctca agcaaggttg gtctatttca tctccgcttc tataccgtgc      360

ctgatactgt tggatgagaa cacgactcaa cttcctgctg ctctgtattg ccagtgtttt      420

gtctgtgatt tggatcggag tcctccttac ttggaatgat aataatcttg gcggaatctc      480

cctaaacgga ggcaaggatt ctgcctatga tgatctgcta tcattgggaa gcttcaacga      540

catggaggtc gactcctatg tcaccaacat ctacgacaat gctccagtgc taggatgtac      600

ggatttgtct tatcatggat tgttgaaagt caccccaaag catgacttag cttgcgattt      660

ggagttcata agagctcaga ttttggacat tgacgtttac tccgccataa aagacttaga      720

agataaagcc ttgactgtaa aacaaaaggt tgaaaaacac tggtttacgt tttatggtag      780

ttcagtcttt ctgcccgaac acgatgtgca ttacctggtt agacgagtca tcttttcggc      840

tgaaggaaag gcgaactctc cagtaacatc                                       870


<210>  18
<211>  1733
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the 3'-Region used for knock out of PpBMT2

<400>  18
ccatatgatg ggtgtttgct cactcgtatg gatcaaaatt ccatggtttc ttctgtacaa       60

cttgtacact tatttggact tttctaacgg tttttctggt gatttgagaa gtccttattt      120

tggtgttcgc agcttatccg tgattgaacc atcagaaata ctgcagctcg ttatctagtt      180

tcagaatgtg ttgtagaata caatcaattc tgagtctagt ttgggtgggt cttggcgacg      240

ggaccgttat atgcatctat gcagtgttaa ggtacataga atgaaaatgt aggggttaat      300

cgaaagcatc gttaatttca gtagaacgta gttctattcc ctacccaaat aatttgccaa      360

gaatgcttcg tatccacata cgcagtggac gtagcaaatt tcactttgga ctgtgacctc      420

aagtcgttat cttctacttg gacattgatg gtcattacgt aatccacaaa gaattggata      480

gcctctcgtt ttatctagtg cacagcctaa tagcacttaa gtaagagcaa tggacaaatt      540

tgcatagaca ttgagctaga tacgtaactc agatcttgtt cactcatggt gtactcgaag      600

tactgctgga accgttacct cttatcattt cgctactggc tcgtgaaact actggatgaa      660

aaaaaaaaaa gagctgaaag cgagatcatc ccattttgtc atcatacaaa ttcacgcttg      720

cagttttgct tcgttaacaa gacaagatgt ctttatcaaa gacccgtttt ttcttcttga      780

agaatacttc cctgttgagc acatgcaaac catatttatc tcagatttca ctcaacttgg      840

gtgcttccaa gagaagtaaa attcttccca ctgcatcaac ttccaagaaa cccgtagacc      900

agtttctctt cagccaaaag aagttgctcg ccgatcaccg cggtaacaga ggagtcagaa      960

ggtttcacac ccttccatcc cgatttcaaa gtcaaagtgc tgcgttgaac caaggttttc     1020

aggttgccaa agcccagtct gcaaaaacta gttccaaatg gcctattaat tcccataaaa     1080

gtgttggcta cgtatgtatc ggtacctcca ttctggtatt tgctattgtt gtcgttggtg     1140

ggttgactag actgaccgaa tccggtcttt ccataacgga gtggaaacct atcactggtt     1200

cggttccccc actgactgag gaagactgga agttggaatt tgaaaaatac aaacaaagcc     1260

ctgagtttca ggaactaaat tctcacataa cattggaaga gttcaagttt atattttcca     1320

tggaatgggg acatagattg ttgggaaggg tcatcggcct gtcgtttgtt cttcccacgt     1380

tttacttcat tgcccgtcga aagtgttcca aagatgttgc attgaaactg cttgcaatat     1440

gctctatgat aggattccaa ggtttcatcg gctggtggat ggtgtattcc ggattggaca     1500

aacagcaatt ggctgaacgt aactccaaac caactgtgtc tccatatcgc ttaactaccc     1560

atcttggaac tgcatttgtt atttactgtt acatgattta cacagggctt caagttttga     1620

agaactataa gatcatgaaa cagcctgaag cgtatgttca aattttcaag caaattgcgt     1680

ctccaaaatt gaaaactttc aagagactct cttcagttct attaggcctg gtg            1733


<210>  19
<211>  981
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encodes MmSLC35A3 UDP-GlcNAc transporter;DNA

<400>  19
atgtctgcca acctaaaata tctttccttg ggaattttgg tgtttcagac taccagtctg       60

gttctaacga tgcggtattc taggacttta aaagaggagg ggcctcgtta tctgtcttct      120

acagcagtgg ttgtggctga atttttgaag ataatggcct gcatcttttt agtctacaaa      180

gacagtaagt gtagtgtgag agcactgaat agagtactgc atgatgaaat tcttaataag      240

cccatggaaa ccctgaagct cgctatcccg tcagggatat atactcttca gaacaactta      300

ctctatgtgg cactgtcaaa cctagatgca gccacttacc aggttacata tcagttgaaa      360

atacttacaa cagcattatt ttctgtgtct atgcttggta aaaaattagg tgtgtaccag      420

tggctctccc tagtaattct gatggcagga gttgcttttg tacagtggcc ttcagattct      480

caagagctga actctaagga cctttcaaca ggctcacagt ttgtaggcct catggcagtt      540

ctcacagcct gtttttcaag tggctttgct ggagtttatt ttgagaaaat cttaaaagaa      600

acaaaacagt cagtatggat aaggaacatt caacttggtt tctttggaag tatatttgga      660

ttaatgggtg tatacgttta tgatggagaa ttggtctcaa agaatggatt ttttcaggga      720

tataatcaac tgacgtggat agttgttgct ctgcaggcac ttggaggcct tgtaatagct      780

gctgtcatca aatatgcaga taacatttta aaaggatttg cgacctcctt atccataata      840

ttgtcaacaa taatatctta tttttggttg caagattttg tgccaaccag tgtctttttc      900

cttggagcca tccttgtaat agcagctact ttcttgtatg gttacgatcc caaacctgca      960

ggaaatccca ctaaagcata g                                                981


<210>  20
<211>  486
<212>  DNA
<213>  Artificial

<220>
<223>  PpGAPDH promoter

<400>  20
tttttgtaga aatgtcttgg tgtcctcgtc caatcaggta gccatctctg aaatatctgg       60

ctccgttgca actccgaacg acctgctggc aacgtaaaat tctccggggt aaaacttaaa      120

tgtggagtaa tggaaccaga aacgtctctt cccttctctc tccttccacc gcccgttacc      180

gtccctagga aattttactc tgctggagag cttcttctac ggcccccttg cagcaatgct      240

cttcccagca ttacgttgcg ggtaaaacgg aggtcgtgta cccgacctag cagcccaggg      300

atggaaaagt cccggccgtc gctggcaata atagcgggcg gacgcatgtc atgagattat      360

tggaaaccac cagaatcgaa tataaaaggc gaacaccttt cccaattttg gtttctcctg      420

acccaaagac tttaaattta atttatttgt ccctatttca atcaattgaa caactatcaa      480

aacaca                                                                 486


<210>  21
<211>  293
<212>  DNA
<213>  Artificial

<220>
<223>  ScCYC TT

<400>  21
acaggcccct tttcctttgt cgatatcatg taattagtta tgtcacgctt acattcacgc       60

cctcctccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc      120

cctatttatt ttttttaata gttatgttag tattaagaac gttatttata tttcaaattt      180

ttcttttttt tctgtacaaa cgcgtgtacg catgtaacat tatactgaaa accttgcttg      240

agaaggtttt gggacgctcg aaggctttaa tttgcaagct gccggctctt aag             293


<210>  22
<211>  1128
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the 5'-Region used for knock out of PpMNN4L1

<400>  22
gatctggcca ttgtgaaact tgacactaaa gacaaaactc ttagagtttc caatcactta       60

ggagacgatg tttcctacaa cgagtacgat ccctcattga tcatgagcaa tttgtatgtg      120

aaaaaagtca tcgaccttga caccttggat aaaagggctg gaggaggtgg aaccacctgt      180

gcaggcggtc tgaaagtgtt caagtacgga tctactacca aatatacatc tggtaacctg      240

aacggcgtca ggttagtata ctggaacgaa ggaaagttgc aaagctccaa atttgtggtt      300

cgatcctcta attactctca aaagcttgga ggaaacagca acgccgaatc aattgacaac      360

aatggtgtgg gttttgcctc agctggagac tcaggcgcat ggattctttc caagctacaa      420

gatgttaggg agtaccagtc attcactgaa aagctaggtg aagctacgat gagcattttc      480

gatttccacg gtcttaaaca ggagacttct actacagggc ttggggtagt tggtatgatt      540

cattcttacg acggtgagtt caaacagttt ggtttgttca ctccaatgac atctattcta      600

caaagacttc aacgagtgac caatgtagaa tggtgtgtag cgggttgcga agatggggat      660

gtggacactg aaggagaaca cgaattgagt gatttggaac aactgcatat gcatagtgat      720

tccgactagt caggcaagag agagccctca aatttacctc tctgcccctc ctcactcctt      780

ttggtacgca taattgcagt ataaagaact tgctgccagc cagtaatctt atttcatacg      840

cagttctata tagcacataa tcttgcttgt atgtatgaaa tttaccgcgt tttagttgaa      900

attgtttatg ttgtgtgcct tgcatgaaat ctctcgttag ccctatcctt acatttaact      960

ggtctcaaaa cctctaccaa ttccattgct gtacaacaat atgaggcggc attactgtag     1020

ggttggaaaa aaattgtcat tccagctaga gatcacacga cttcatcacg cttattgctc     1080

ctcattgcta aatcatttac tcttgacttc gacccagaaa agttcgcc                  1128


<210>  23
<211>  1231
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the 3'-Region used for knock out of PpMNN4L1

<400>  23
gcatgtcaaa cttgaacaca acgactagat agttgttttt tctatataaa acgaaacgtt       60

atcatcttta ataatcattg aggtttaccc ttatagttcc gtattttcgt ttccaaactt      120

agtaatcttt tggaaatatc atcaaagctg gtgccaatct tcttgtttga agtttcaaac      180

tgctccacca agctacttag agactgttct aggtctgaag caacttcgaa cacagagaca      240

gctgccgccg attgttcttt tttgtgtttt tcttctggaa gaggggcatc atcttgtatg      300

tccaatgccc gtatcctttc tgagttgtcc gacacattgt ccttcgaaga gtttcctgac      360

attgggcttc ttctatccgt gtattaattt tgggttaagt tcctcgtttg catagcagtg      420

gatacctcga tttttttggc tcctatttac ctgacataat attctactat aatccaactt      480

ggacgcgtca tctatgataa ctaggctctc ctttgttcaa aggggacgtc ttcataatcc      540

actggcacga agtaagtctg caacgaggcg gcttttgcaa cagaacgata gtgtcgtttc      600

gtacttggac tatgctaaac aaaaggatct gtcaaacatt tcaaccgtgt ttcaaggcac      660

tctttacgaa ttatcgacca agaccttcct agacgaacat ttcaacatat ccaggctact      720

gcttcaaggt ggtgcaaatg ataaaggtat agatattaga tgtgtttggg acctaaaaca      780

gttcttgcct gaagattccc ttgagcaaca ggcttcaata gccaagttag agaagcagta      840

ccaaatcggt aacaaaaggg ggaagcatat aaaaccttta ctattgcgac aaaatccatc      900

cttgaaagta aagctgtttg ttcaatgtaa agcatacgaa acgaaggagg tagatcctaa      960

gatggttaga gaacttaacg ggacatactc cagctgcatc ccatattacg atcgctggaa     1020

gacttttttc atgtacgtat cgcccaccaa cctttcaaag caagctaggt atgattttga     1080

cagttctcac aatccattgg ttttcatgca acttgaaaaa acccaactca aacttcatgg     1140

ggatccatac aatgtaaatc attacgagag ggcgaggttg aaaagtttcc attgcaatca     1200

cgtcgcatca tggctactga aaggccttaa c                                    1231


<210>  24
<211>  937
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the 5'-Region used for knock out of PpPNO1 and PpMNN4

<400>  24
tcattctata tgttcaagaa aagggtagtg aaaggaaaga aaaggcatat aggcgaggga       60

gagttagcta gcatacaaga taatgaagga tcaatagcgg tagttaaagt gcacaagaaa      120

agagcacctg ttgaggctga tgataaagct ccaattacat tgccacagag aaacacagta      180

acagaaatag gaggggatgc accacgagaa gagcattcag tgaacaactt tgccaaattc      240

ataaccccaa gcgctaataa gccaatgtca aagtcggcta ctaacattaa tagtacaaca      300

actatcgatt ttcaaccaga tgtttgcaag gactacaaac agacaggtta ctgcggatat      360

ggtgacactt gtaagttttt gcacctgagg gatgatttca aacagggatg gaaattagat      420

agggagtggg aaaatgtcca aaagaagaag cataatactc tcaaaggggt taaggagatc      480

caaatgttta atgaagatga gctcaaagat atcccgttta aatgcattat atgcaaagga      540

gattacaaat cacccgtgaa aacttcttgc aatcattatt tttgcgaaca atgtttcctg      600

caacggtcaa gaagaaaacc aaattgtatt atatgtggca gagacacttt aggagttgct      660

ttaccagcaa agaagttgtc ccaatttctg gctaagatac ataataatga aagtaataaa      720

gtttagtaat tgcattgcgt tgactattga ttgcattgat gtcgtgtgat actttcaccg      780

aaaaaaaaca cgaagcgcaa taggagcggt tgcatattag tccccaaagc tatttaattg      840

tgcctgaaac tgttttttaa gctcatcaag cataattgta tgcattgcga cgtaaccaac      900

gtttaggcgc agtttaatca tagcccactg ctaagcc                               937


<210>  25
<211>  1906
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the 3'-Region used for knock out of PpPNO1 and PpMNN4

<400>  25
cggaggaatg caaataataa tctccttaat tacccactga taagctcaag agacgcggtt       60

tgaaaacgat ataatgaatc atttggattt tataataaac cctgacagtt tttccactgt      120

attgttttaa cactcattgg aagctgtatt gattctaaga agctagaaat caatacggcc      180

atacaaaaga tgacattgaa taagcaccgg cttttttgat tagcatatac cttaaagcat      240

gcattcatgg ctacatagtt gttaaagggc ttcttccatt atcagtataa tgaattacat      300

aatcatgcac ttatatttgc ccatctctgt tctctcactc ttgcctgggt atattctatg      360

aaattgcgta tagcgtgtct ccagttgaac cccaagcttg gcgagtttga agagaatgct      420

aaccttgcgt attccttgct tcaggaaaca ttcaaggaga aacaggtcaa gaagccaaac      480

attttgatcc ttcccgagtt agcattgact ggctacaatt ttcaaagcca gcagcggata      540

gagccttttt tggaggaaac aaccaaggga gctagtaccc aatgggctca aaaagtatcc      600

aagacgtggg attgctttac tttaatagga tacccagaaa aaagtttaga gagccctccc      660

cgtatttaca acagtgcggt acttgtatcg cctcagggaa aagtaatgaa caactacaga      720

aagtccttct tgtatgaagc tgatgaacat tggggatgtt cggaatcttc tgatgggttt      780

caaacagtag atttattaat tgaaggaaag actgtaaaga catcatttgg aatttgcatg      840

gatttgaatc cttataaatt tgaagctcca ttcacagact tcgagttcag tggccattgc      900

ttgaaaaccg gtacaagact cattttgtgc ccaatggcct ggttgtcccc tctatcgcct      960

tccattaaaa aggatcttag tgatatagag aaaagcagac ttcaaaagtt ctaccttgaa     1020

aaaatagata ccccggaatt tgacgttaat tacgaattga aaaaagatga agtattgccc     1080

acccgtatga atgaaacgtt ggaaacaatt gactttgagc cttcaaaacc ggactactct     1140

aatataaatt attggatact aaggtttttt ccctttctga ctcatgtcta taaacgagat     1200

gtgctcaaag agaatgcagt tgcagtctta tgcaaccgag ttggcattga gagtgatgtc     1260

ttgtacggag gatcaaccac gattctaaac ttcaatggta agttagcatc gacacaagag     1320

gagctggagt tgtacgggca gactaatagt ctcaacccca gtgtggaagt attgggggcc     1380

cttggcatgg gtcaacaggg aattctagta cgagacattg aattaacata atatacaata     1440

tacaataaac acaaataaag aatacaagcc tgacaaaaat tcacaaatta ttgcctagac     1500

ttgtcgttat cagcagcgac ctttttccaa tgctcaattt cacgatatgc cttttctagc     1560

tctgctttaa gcttctcatt ggaattggct aactcgttga ctgcttggtc agtgatgagt     1620

ttctccaagg tccatttctc gatgttgttg ttttcgtttt cctttaatct cttgatataa     1680

tcaacagcct tctttaatat ctgagccttg ttcgagtccc ctgttggcaa cagagcggcc     1740

agttccttta ttccgtggtt tatattttct cttctacgcc tttctacttc tttgtgattc     1800

tctttacgca tcttatgcca ttcttcagaa ccagtggctg gcttaaccga atagccagag     1860

cctgaagaag ccgcactaga agaagcagtg gcattgttga ctatgg                    1906


<210>  26
<211>  1224
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encodes human GnTI catalytic domain (NA) Codon-optimized

<400>  26
tcagtcagtg ctcttgatgg tgacccagca agtttgacca gagaagtgat tagattggcc       60

caagacgcag aggtggagtt ggagagacaa cgtggactgc tgcagcaaat cggagatgca      120

ttgtctagtc aaagaggtag ggtgcctacc gcagctcctc cagcacagcc tagagtgcat      180

gtgacccctg caccagctgt gattcctatc ttggtcatcg cctgtgacag atctactgtt      240

agaagatgtc tggacaagct gttgcattac agaccatctg ctgagttgtt ccctatcatc      300

gttagtcaag actgtggtca cgaggagact gcccaagcca tcgcctccta cggatctgct      360

gtcactcaca tcagacagcc tgacctgtca tctattgctg tgccaccaga ccacagaaag      420

ttccaaggtt actacaagat cgctagacac tacagatggg cattgggtca agtcttcaga      480

cagtttagat tccctgctgc tgtggtggtg gaggatgact tggaggtggc tcctgacttc      540

tttgagtact ttagagcaac ctatccattg ctgaaggcag acccatccct gtggtgtgtc      600

tctgcctgga atgacaacgg taaggagcaa atggtggacg cttctaggcc tgagctgttg      660

tacagaaccg acttctttcc tggtctggga tggttgctgt tggctgagtt gtgggctgag      720

ttggagccta agtggccaaa ggcattctgg gacgactgga tgagaagacc tgagcaaaga      780

cagggtagag cctgtatcag acctgagatc tcaagaacca tgacctttgg tagaaaggga      840

gtgtctcacg gtcaattctt tgaccaacac ttgaagttta tcaagctgaa ccagcaattt      900

gtgcacttca cccaactgga cctgtcttac ttgcagagag aggcctatga cagagatttc      960

ctagctagag tctacggagc tcctcaactg caagtggaga aagtgaggac caatgacaga     1020

aaggagttgg gagaggtgag agtgcagtac actggtaggg actcctttaa ggctttcgct     1080

aaggctctgg gtgtcatgga tgaccttaag tctggagttc ctagagctgg ttacagaggt     1140

attgtcacct ttcaattcag aggtagaaga gtccacttgg ctcctccacc tacttgggag     1200

ggttatgatc cttcttggaa ttag                                            1224


<210>  27
<211>  99
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encodes Pp SEC12 (10)

<400>  27
atgcccagaa aaatatttaa ctacttcatt ttgactgtat tcatggcaat tcttgctatt       60

gttttacaat ggtctataga gaatggacat gggcgcgcc                              99


<210>  28
<211>  435
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the PpSEC4 promoter

<400>  28
gaagtaaagt tggcgaaact ttgggaacct ttggttaaaa ctttgtaatt tttgtcgcta       60

cccattaggc agaatctgca tcttgggagg gggatgtggt ggcgttctga gatgtacgcg      120

aagaatgaag agccagtggt aacaacaggc ctagagagat acgggcataa tgggtataac      180

ctacaagtta agaatgtagc agccctggaa accagattga aacgaaaaac gaaatcattt      240

aaactgtagg atgttttggc tcattgtctg gaaggctggc tgtttattgc cctgttcttt      300

gcatgggaat aagctattat atccctcaca taatcccaga aaatagattg aagcaacgcg      360

aaatccttac gtatcgaagt agccttctta cacattcacg ttgtacggat aagaaaacta      420

ctcaaacgaa caatc                                                       435


<210>  29
<211>  404
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the PpOCH1 terminator

<400>  29
aatagatata gcgagattag agaatgaata ccttcttcta agcgatcgtc cgtcatcata       60

gaatatcatg gactgtatag tttttttttt gtacatataa tgattaaacg gtcatccaac      120

atctcgttga cagatctctc agtacgcgaa atccctgact atcaaagcaa gaaccgatga      180

agaaaaaaac aacagtaacc caaacaccac aacaaacact ttatcttctc ccccccaaca      240

ccaatcatca aagagatgtc ggaacacaaa caccaagaag caaaaactaa ccccatataa      300

aaacatcctg gtagataatg ctggtaaccc gctctccttc catattctgg gctacttcac      360

gaagtctgac cggtctcagt tgatcaacat gatcctcgaa atgg                       404


<210>  30
<211>  1407
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encodes Mm ManI catalytic domain (FB)

<400>  30
gagcccgctg acgccaccat ccgtgagaag agggcaaaga tcaaagagat gatgacccat       60

gcttggaata attataaacg ctatgcgtgg ggcttgaacg aactgaaacc tatatcaaaa      120

gaaggccatt caagcagttt gtttggcaac atcaaaggag ctacaatagt agatgccctg      180

gatacccttt tcattatggg catgaagact gaatttcaag aagctaaatc gtggattaaa      240

aaatatttag attttaatgt gaatgctgaa gtttctgttt ttgaagtcaa catacgcttc      300

gtcggtggac tgctgtcagc ctactatttg tccggagagg agatatttcg aaagaaagca      360

gtggaacttg gggtaaaatt gctacctgca tttcatactc cctctggaat accttgggca      420

ttgctgaata tgaaaagtgg gatcgggcgg aactggccct gggcctctgg aggcagcagt      480

atcctggccg aatttggaac tctgcattta gagtttatgc acttgtccca cttatcagga      540

gacccagtct ttgccgaaaa ggttatgaaa attcgaacag tgttgaacaa actggacaaa      600

ccagaaggcc tttatcctaa ctatctgaac cccagtagtg gacagtgggg tcaacatcat      660

gtgtcggttg gaggacttgg agacagcttt tatgaatatt tgcttaaggc gtggttaatg      720

tctgacaaga cagatctcga agccaagaag atgtattttg atgctgttca ggccatcgag      780

actcacttga tccgcaagtc aagtggggga ctaacgtaca tcgcagagtg gaaggggggc      840

ctcctggaac acaagatggg ccacctgacg tgctttgcag gaggcatgtt tgcacttggg      900

gcagatggag ctccggaagc ccgggcccaa cactaccttg aactcggagc tgaaattgcc      960

cgcacttgtc atgaatctta taatcgtaca tatgtgaagt tgggaccgga agcgtttcga     1020

tttgatggcg gtgtggaagc tattgccacg aggcaaaatg aaaagtatta catcttacgg     1080

cccgaggtca tcgagacata catgtacatg tggcgactga ctcacgaccc caagtacagg     1140

acctgggcct gggaagccgt ggaggctcta gaaagtcact gcagagtgaa cggaggctac     1200

tcaggcttac gggatgttta cattgcccgt gagagttatg acgatgtcca gcaaagtttc     1260

ttcctggcag agacactgaa gtatttgtac ttgatatttt ccgatgatga ccttcttcca     1320

ctagaacact ggatcttcaa caccgaggct catcctttcc ctatactccg tgaacagaag     1380

aaggaaattg atggcaaaga gaaatga                                         1407


<210>  31
<211>  318
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encodes ScSEC12 (8)

<400>  31
atgaacacta tccacataat aaaattaccg cttaactacg ccaactacac ctcaatgaaa       60

caaaaaatct ctaaattttt caccaacttc atccttattg tgctgctttc ttacatttta      120

cagttctcct ataagcacaa tttgcattcc atgcttttca attacgcgaa ggacaatttt      180

ctaacgaaaa gagacaccat ctcttcgccc tacgtagttg atgaagactt acatcaaaca      240

actttgtttg gcaaccacgg tacaaaaaca tctgtaccta gcgtagattc cataaaagtg      300

catggcgtgg ggcgcgcc                                                    318


<210>  32
<211>  1250
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the 5'-region that was used to knock into the PpADE1 
       locus

<400>  32
gagtcggcca agagatgata actgttacta agcttctccg taattagtgg tattttgtaa       60

cttttaccaa taatcgttta tgaatacgga tatttttcga ccttatccag tgccaaatca      120

cgtaacttaa tcatggttta aatactccac ttgaacgatt cattattcag aaaaaagtca      180

ggttggcaga aacacttggg cgctttgaag agtataagag tattaagcat taaacatctg      240

aactttcacc gccccaatat actactctag gaaactcgaa aaattccttt ccatgtgtca      300

tcgcttccaa cacactttgc tgtatccttc caagtatgtc cattgtgaac actgatctgg      360

acggaatcct acctttaatc gccaaaggaa aggttagaga catttatgca gtcgatgaga      420

acaacttgct gttcgtcgca actgaccgta tctccgctta cgatgtgatt atgacaaacg      480

gtattcctga taagggaaag attttgactc agctctcagt tttctggttt gattttttgg      540

caccctacat aaagaatcat ttggttgctt ctaatgacaa ggaagtcttt gctttactac      600

catcaaaact gtctgaagaa aaatacaaat ctcaattaga gggacgatcc ttgatagtaa      660

aaaagcacag actgatacct ttggaagcca ttgtcagagg ttacatcact ggaagtgcat      720

ggaaagagta caagaactca aaaactgtcc atggagtcaa ggttgaaaac gagaaccttc      780

aagagagcga cgcctttcca actccgattt tcacaccttc aacgaaagct gaacagggtg      840

aacacgatga aaacatctct attgaacaag ctgctgagat tgtaggtaaa gacatttgtg      900

agaaggtcgc tgtcaaggcg gtcgagttgt attctgctgc aaaaaacctc gcccttttga      960

aggggatcat tattgctgat acgaaattcg aatttggact ggacgaaaac aatgaattgg     1020

tactagtaga tgaagtttta actccagatt cttctagatt ttggaatcaa aagacttacc     1080

aagtgggtaa atcgcaagag agttacgata agcagtttct cagagattgg ttgacggcca     1140

acggattgaa tggcaaagag ggcgtagcca tggatgcaga aattgctatc aagagtaaag     1200

aaaagtatat tgaagcttat gaagcaatta ctggcaagaa atgggcttga                1250


<210>  33
<211>  376
<212>  DNA
<213>  Artificial

<220>
<223>  PpALG3 TT

<400>  33
atttacaatt agtaatatta aggtggtaaa aacattcgta gaattgaaat gaattaatat       60

agtatgacaa tggttcatgt ctataaatct ccggcttcgg taccttctcc ccaattgaat      120

acattgtcaa aatgaatggt tgaactatta ggttcgccag tttcgttatt aagaaaactg      180

ttaaaatcaa attccatatc atcggttcca gtgggaggac cagttccatc gccaaaatcc      240

tgtaagaatc cattgtcaga acctgtaaag tcagtttgag atgaaatttt tccggtcttt      300

gttgacttgg aagcttcgtt aaggttaggt gaaacagttt gatcaaccag cggctcccgt      360

tttcgtcgct tagtag                                                      376


<210>  34
<211>  882
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the 3'-region that was used to knock into the PpADE1 
       locus

<400>  34
atgattagta ccctcctcgc ctttttcaga catctgaaat ttcccttatt cttccaattc       60

catataaaat cctatttagg taattagtaa acaatgatca taaagtgaaa tcattcaagt      120

aaccattccg tttatcgttg atttaaaatc aataacgaat gaatgtcggt ctgagtagtc      180

aatttgttgc cttggagctc attggcaggg ggtcttttgg ctcagtatgg aaggttgaaa      240

ggaaaacaga tggaaagtgg ttcgtcagaa aagaggtatc ctacatgaag atgaatgcca      300

aagagatatc tcaagtgata gctgagttca gaattcttag tgagttaagc catcccaaca      360

ttgtgaagta ccttcatcac gaacatattt ctgagaataa aactgtcaat ttatacatgg      420

aatactgtga tggtggagat ctctccaagc tgattcgaac acatagaagg aacaaagagt      480

acatttcaga agaaaaaata tggagtattt ttacgcaggt tttattagca ttgtatcgtt      540

gtcattatgg aactgatttc acggcttcaa aggagtttga atcgctcaat aaaggtaata      600

gacgaaccca gaatccttcg tgggtagact cgacaagagt tattattcac agggatataa      660

aacccgacaa catctttctg atgaacaatt caaaccttgt caaactggga gattttggat      720

tagcaaaaat tctggaccaa gaaaacgatt ttgccaaaac atacgtcggt acgccgtatt      780

acatgtctcc tgaagtgctg ttggaccaac cctactcacc attatgtgat atatggtctc      840

ttgggtgcgt catgtatgag ctatgtgcat tgaggcctcc tt                         882


<210>  35
<211>  2100
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encodes ScGAL10

<400>  35
atgacagctc agttacaaag tgaaagtact tctaaaattg ttttggttac aggtggtgct       60

ggatacattg gttcacacac tgtggtagag ctaattgaga atggatatga ctgtgttgtt      120

gctgataacc tgtcgaattc aacttatgat tctgtagcca ggttagaggt cttgaccaag      180

catcacattc ccttctatga ggttgatttg tgtgaccgaa aaggtctgga aaaggttttc      240

aaagaatata aaattgattc ggtaattcac tttgctggtt taaaggctgt aggtgaatct      300

acacaaatcc cgctgagata ctatcacaat aacattttgg gaactgtcgt tttattagag      360

ttaatgcaac aatacaacgt ttccaaattt gttttttcat cttctgctac tgtctatggt      420

gatgctacga gattcccaaa tatgattcct atcccagaag aatgtccctt agggcctact      480

aatccgtatg gtcatacgaa atacgccatt gagaatatct tgaatgatct ttacaatagc      540

gacaaaaaaa gttggaagtt tgctatcttg cgttatttta acccaattgg cgcacatccc      600

tctggattaa tcggagaaga tccgctaggt ataccaaaca atttgttgcc atatatggct      660

caagtagctg ttggtaggcg cgagaagctt tacatcttcg gagacgatta tgattccaga      720

gatggtaccc cgatcaggga ttatatccac gtagttgatc tagcaaaagg tcatattgca      780

gccctgcaat acctagaggc ctacaatgaa aatgaaggtt tgtgtcgtga gtggaacttg      840

ggttccggta aaggttctac agtttttgaa gtttatcatg cattctgcaa agcttctggt      900

attgatcttc catacaaagt tacgggcaga agagcaggtg atgttttgaa cttgacggct      960

aaaccagata gggccaaacg cgaactgaaa tggcagaccg agttgcaggt tgaagactcc     1020

tgcaaggatt tatggaaatg gactactgag aatccttttg gttaccagtt aaggggtgtc     1080

gaggccagat tttccgctga agatatgcgt tatgacgcaa gatttgtgac tattggtgcc     1140

ggcaccagat ttcaagccac gtttgccaat ttgggcgcca gcattgttga cctgaaagtg     1200

aacggacaat cagttgttct tggctatgaa aatgaggaag ggtatttgaa tcctgatagt     1260

gcttatatag gcgccacgat cggcaggtat gctaatcgta tttcgaaggg taagtttagt     1320

ttatgcaaca aagactatca gttaaccgtt aataacggcg ttaatgcgaa tcatagtagt     1380

atcggttctt tccacagaaa aagatttttg ggacccatca ttcaaaatcc ttcaaaggat     1440

gtttttaccg ccgagtacat gctgatagat aatgagaagg acaccgaatt tccaggtgat     1500

ctattggtaa ccatacagta tactgtgaac gttgcccaaa aaagtttgga aatggtatat     1560

aaaggtaaat tgactgctgg tgaagcgacg ccaataaatt taacaaatca tagttatttc     1620

aatctgaaca agccatatgg agacactatt gagggtacgg agattatggt gcgttcaaaa     1680

aaatctgttg atgtcgacaa aaacatgatt cctacgggta atatcgtcga tagagaaatt     1740

gctaccttta actctacaaa gccaacggtc ttaggcccca aaaatcccca gtttgattgt     1800

tgttttgtgg tggatgaaaa tgctaagcca agtcaaatca atactctaaa caatgaattg     1860

acgcttattg tcaaggcttt tcatcccgat tccaatatta cattagaagt tttaagtaca     1920

gagccaactt atcaatttta taccggtgat ttcttgtctg ctggttacga agcaagacaa     1980

ggttttgcaa ttgagcctgg tagatacatt gatgctatca atcaagagaa ctggaaagat     2040

tgtgtaacct tgaaaaacgg tgaaacttac gggtccaaga ttgtctacag attttcctga     2100


<210>  36
<211>  1037
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the PpPMA1 promoter

<400>  36
aaatgcgtac ctcttctacg agattcaagc gaatgagaat aatgtaatat gcaagatcag       60

aaagaatgaa aggagttgaa aaaaaaaacc gttgcgtttt gaccttgaat ggggtggagg      120

tttccattca aagtaaagcc tgtgtcttgg tattttcggc ggcacaagaa atcgtaattt      180

tcatcttcta aacgatgaag atcgcagccc aacctgtatg tagttaaccg gtcggaatta      240

taagaaagat tttcgatcaa caaaccctag caaatagaaa gcagggttac aactttaaac      300

cgaagtcaca aacgataaac cactcagctc ccacccaaat tcattcccac tagcagaaag      360

gaattattta atccctcagg aaacctcgat gattctcccg ttcttccatg ggcgggtatc      420

gcaaaatgag gaatttttca aatttctcta ttgtcaagac tgtttattat ctaagaaata      480

gcccaatccg aagctcagtt ttgaaaaaat cacttccgcg tttctttttt acagcccgat      540

gaatatccaa atttggaata tggattactc tatcgggact gcagataata tgacaacaac      600

gcagattaca ttttaggtaa ggcataaaca ccagccagaa atgaaacgcc cactagccat      660

ggtcgaatag tccaatgaat tcagatagct atggtctaaa agctgatgtt ttttattggg      720

taatggcgaa gagtccagta cgacttccag cagagctgag atggccattt ttgggggtat      780

tagtaacttt ttgagctctt ttcacttcga tgaagtgtcc cattcgggat ataatcggat      840

cgcgtcgttt tctcgaaaat acagcttagc gtcgtccgct tgttgtaaaa gcagcaccac      900

attcctaatc tcttatataa acaaaacaac ccaaattatc agtgctgttt tcccaccaga      960

tataagtttc ttttctcttc cgctttttga ttttttatct ctttccttta aaaacttctt     1020

taccttaaag ggcggcc                                                    1037


<210>  37
<211>  512
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the PpPMA1 terminator

<400>  37
taagcttcac gatttgtgtt ccagtttatc ccccctttat ataccgttaa ccctttccct       60

gttgagctga ctgttgttgt attaccgcaa tttttccaag tttgccatgc ttttcgtgtt      120

atttgaccga tgtctttttt cccaaatcaa actatatttg ttaccattta aaccaagtta      180

tcttttgtat taagagtcta agtttgttcc caggcttcat gtgagagtga taaccatcca      240

gactatgatt cttgtttttt attgggtttg tttgtgtgat acatctgagt tgtgattcgt      300

aaagtatgtc agtctatcta gatttttaat agttaattgg taatcaatga cttgtttgtt      360

ttaactttta aattgtgggt cgtatccacg cgtttagtat agctgttcat ggctgttaga      420

ggagggcgat gtttatatac agaggacaag aatgaggagg cggcgtgtat ttttaaaatg      480

gagacgcgac tcctgtacac cttatcggtt gg                                    512


<210>  38
<211>  1068
<212>  DNA
<213>  Artificial

<220>
<223>  hGalT codon optimized (XB)

<400>  38
ggtagagatt tgtctagatt gccacagttg gttggtgttt ccactccatt gcaaggaggt       60

tctaactctg ctgctgctat tggtcaatct tccggtgagt tgagaactgg tggagctaga      120

ccacctccac cattgggagc ttcctctcaa ccaagaccag gtggtgattc ttctccagtt      180

gttgactctg gtccaggtcc agcttctaac ttgacttccg ttccagttcc acacactact      240

gctttgtcct tgccagcttg tccagaagaa tccccattgt tggttggtcc aatgttgatc      300

gagttcaaca tgccagttga cttggagttg gttgctaagc agaacccaaa cgttaagatg      360

ggtggtagat acgctccaag agactgtgtt tccccacaca aagttgctat catcatccca      420

ttcagaaaca gacaggagca cttgaagtac tggttgtact acttgcaccc agttttgcaa      480

agacagcagt tggactacgg tatctacgtt atcaaccagg ctggtgacac tattttcaac      540

agagctaagt tgttgaatgt tggtttccag gaggctttga aggattacga ctacacttgt      600

ttcgttttct ccgacgttga cttgattcca atgaacgacc acaacgctta cagatgtttc      660

tcccagccaa gacacatttc tgttgctatg gacaagttcg gtttctcctt gccatacgtt      720

caatacttcg gtggtgtttc cgctttgtcc aagcagcagt tcttgactat caacggtttc      780

ccaaacaatt actggggatg gggtggtgaa gatgacgaca tctttaacag attggttttc      840

agaggaatgt ccatctctag accaaacgct gttgttggta gatgtagaat gatcagacac      900

tccagagaca agaagaacga gccaaaccca caaagattcg acagaatcgc tcacactaag      960

gaaactatgt tgtccgacgg attgaactcc ttgacttacc aggttttgga cgttcagaga     1020

tacccattgt acactcagat cactgttgac atcggtactc catcctag                  1068


<210>  39
<211>  183
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encodes ScMnt1 (Kre2) (33)

<400>  39
atggccctct ttctcagtaa gagactgttg agatttaccg tcattgcagg tgcggttatt       60

gttctcctcc taacattgaa ttccaacagt agaactcagc aatatattcc gagttccatc      120

tccgctgcat ttgattttac ctcaggatct atatcccctg aacaacaagt catcgggcgc      180

gcc                                                                    183


<210>  40
<211>  1074
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encodes DmUGT

<400>  40
atgaatagca tacacatgaa cgccaatacg ctgaagtaca tcagcctgct gacgctgacc       60

ctgcagaatg ccatcctggg cctcagcatg cgctacgccc gcacccggcc aggcgacatc      120

ttcctcagct ccacggccgt actcatggca gagttcgcca aactgatcac gtgcctgttc      180

ctggtcttca acgaggaggg caaggatgcc cagaagtttg tacgctcgct gcacaagacc      240

atcattgcga atcccatgga cacgctgaag gtgtgcgtcc cctcgctggt ctatatcgtt      300

caaaacaatc tgctgtacgt ctctgcctcc catttggatg cggccaccta ccaggtgacg      360

taccagctga agattctcac cacggccatg ttcgcggttg tcattctgcg ccgcaagctg      420

ctgaacacgc agtggggtgc gctgctgctc ctggtgatgg gcatcgtcct ggtgcagttg      480

gcccaaacgg agggtccgac gagtggctca gccggtggtg ccgcagctgc agccacggcc      540

gcctcctctg gcggtgctcc cgagcagaac aggatgctcg gactgtgggc cgcactgggc      600

gcctgcttcc tctccggatt cgcgggcatc tactttgaga agatcctcaa gggtgccgag      660

atctccgtgt ggatgcggaa tgtgcagttg agtctgctca gcattccctt cggcctgctc      720

acctgtttcg ttaacgacgg cagtaggatc ttcgaccagg gattcttcaa gggctacgat      780

ctgtttgtct ggtacctggt cctgctgcag gccggcggtg gattgatcgt tgccgtggtg      840

gtcaagtacg cggataacat tctcaagggc ttcgccacct cgctggccat catcatctcg      900

tgcgtggcct ccatatacat cttcgacttc aatctcacgc tgcagttcag cttcggagct      960

ggcctggtca tcgcctccat atttctctac ggctacgatc cggccaggtc ggcgccgaag     1020

ccaactatgc atggtcctgg cggcgatgag gagaagctgc tgccgcgcgt ctag           1074


<210>  41
<211>  798
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the PpOCH1 promoter

<400>  41
tggacacagg agactcagaa acagacacag agcgttctga gtcctggtgc tcctgacgta       60

ggcctagaac aggaattatt ggctttattt gtttgtccat ttcataggct tggggtaata      120

gatagatgac agagaaatag agaagaccta atattttttg ttcatggcaa atcgcgggtt      180

cgcggtcggg tcacacacgg agaagtaatg agaagagctg gtaatctggg gtaaaagggt      240

tcaaaagaag gtcgcctggt agggatgcaa tacaaggttg tcttggagtt tacattgacc      300

agatgatttg gctttttctc tgttcaattc acatttttca gcgagaatcg gattgacgga      360

gaaatggcgg ggtgtggggt ggatagatgg cagaaatgct cgcaatcacc gcgaaagaaa      420

gactttatgg aatagaacta ctgggtggtg taaggattac atagctagtc caatggagtc      480

cgttggaaag gtaagaagaa gctaaaaccg gctaagtaac tagggaagaa tgatcagact      540

ttgatttgat gaggtctgaa aatactctgc tgctttttca gttgcttttt ccctgcaacc      600

tatcattttc cttttcataa gcctgccttt tctgttttca cttatatgag ttccgccgag      660

acttccccaa attctctcct ggaacattct ctatcgctct ccttccaagt tgcgccccct      720

ggcactgcct agtaatatta ccacgcgact tatattcagt tccacaattt ccagtgttcg      780

tagcaaatat catcagcc                                                    798


<210>  42
<211>  302
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the PpALG12 terminator

<400>  42
aatatatacc tcatttgttc aatttggtgt aaagagtgtg gcggatagac ttcttgtaaa       60

tcaggaaagc tacaattcca attgctgcaa aaaataccaa tgcccataaa ccagtatgag      120

cggtgccttc gacggattgc ttactttccg accctttgtc gtttgattct tctgcctttg      180

gtgagtcagt ttgtttcgac tttatatctg actcatcaac ttcctttacg gttgcgtttt      240

taatcataat tttagccgtt ggcttattat cccttgagtt ggtaggagtt ttgatgatgc      300

tg                                                                     302


<210>  43
<211>  461
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the 5'-Region used for knock out of PpHIS1

<400>  43
taactggccc tttgacgttt ctgacaatag ttctagagga gtcgtccaaa aactcaactc       60

tgacttgggt gacaccacca cgggatccgg ttcttccgag gaccttgatg accttggcta      120

atgtaactgg agttttagta tccattttaa gatgtgtgtt tctgtaggtt ctgggttgga      180

aaaaaatttt agacaccaga agagaggagt gaactggttt gcgtgggttt agactgtgta      240

aggcactact ctgtcgaagt tttagatagg ggttacccgc tccgatgcat gggaagcgat      300

tagcccggct gttgcccgtt tggtttttga agggtaattt tcaatatctc tgtttgagtc      360

atcaatttca tattcaaaga ttcaaaaaca aaatctggtc caaggagcgc atttaggatt      420

atggagttgg cgaatcactt gaacgataga ctattatttg c                          461


<210>  44
<211>  1841
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the 3'-Region used for knock out of PpHIS1

<400>  44
gtgacattct tgtctttgag atcagtaatt gtagagcata gatagaataa tattcaagac       60

caacggcttc tcttcggaag ctccaagtag cttatagtga tgagtaccgg catatattta      120

taggcttaaa atttcgaggg ttcactatat tcgtttagtg ggaagagttc ctttcactct      180

tgttatctat attgtcagcg tggactgttt ataactgtac caacttagtt tctttcaact      240

ccaggttaag agacataaat gtcctttgat gctgacaata atcagtggaa ttcaaggaag      300

gacaatcccg acctcaatct gttcattaat gaagagttcg aatcgtcctt aaatcaagcg      360

ctagactcaa ttgtcaatga gaaccctttc tttgaccaag aaactataaa tagatcgaat      420

gacaaagttg gaaatgagtc cattagctta catgatattg agcaggcaga ccaaaataaa      480

ccgtcctttg agagcgatat tgatggttcg gcgccgttga taagagacga caaattgcca      540

aagaaacaaa gctgggggct gagcaatttt ttttcaagaa gaaatagcat atgtttacca      600

ctacatgaaa atgattcaag tgttgttaag accgaaagat ctattgcagt gggaacaccc      660

catcttcaat actgcttcaa tggaatctcc aatgccaagt acaatgcatt tacctttttc      720

ccagtcatcc tatacgagca attcaaattt tttttcaatt tatactttac tttagtggct      780

ctctctcaag cgataccgca acttcgcatt ggatatcttt cttcgtatgt cgtcccactt      840

ttgtttgtac tcatagtgac catgtcaaaa gaggcgatgg atgatattca acgccgaaga      900

agggatagag aacagaacaa tgaaccatat gaggttctgt ccagcccatc accagttttg      960

tccaaaaact taaaatgtgg tcacttggtt cgattgcata agggaatgag agtgcccgca     1020

gatatggttc ttgtccagtc aagcgaatcc accggagagt catttatcaa gacagatcag     1080

ctggatggtg agactgattg gaagcttcgg attgtttctc cagttacaca atcgttacca     1140

atgactgaac ttcaaaatgt cgccatcact gcaagcgcac cctcaaaatc aattcactcc     1200

tttcttggaa gattgaccta caatgggcaa tcatatggtc ttacgataga caacacaatg     1260

tggtgtaata ctgtattagc ttctggttca gcaattggtt gtataattta cacaggtaaa     1320

gatactcgac aatcgatgaa cacaactcag cccaaactga aaacgggctt gttagaactg     1380

gaaatcaata gtttgtccaa gatcttatgt gtttgtgtgt ttgcattatc tgtcatctta     1440

gtgctattcc aaggaatagc tgatgattgg tacgtcgata tcatgcggtt tctcattcta     1500

ttctccacta ttatcccagt gtctctgaga gttaaccttg atcttggaaa gtcagtccat     1560

gctcatcaaa tagaaactga tagctcaata cctgaaaccg ttgttagaac tagtacaata     1620

ccggaagacc tgggaagaat tgaataccta ttaagtgaca aaactggaac tcttactcaa     1680

aatgatatgg aaatgaaaaa actacaccta ggaacagtct cttatgctgg tgataccatg     1740

gatattattt ctgatcatgt taaaggtctt aataacgcta aaacatcgag gaaagatctt     1800

ggtatgagaa taagagattt ggttacaact ctggccatct g                         1841


<210>  45
<211>  3105
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encodes Drosophila melanogaster ManII codon-optimized (KD)

<400>  45
agagacgatc caattagacc tccattgaag gttgctagat ccccaagacc aggtcaatgt       60

caagatgttg ttcaggacgt cccaaacgtt gatgtccaga tgttggagtt gtacgataga      120

atgtccttca aggacattga tggtggtgtt tggaagcagg gttggaacat taagtacgat      180

ccattgaagt acaacgctca tcacaagttg aaggtcttcg ttgtcccaca ctcccacaac      240

gatcctggtt ggattcagac cttcgaggaa tactaccagc acgacaccaa gcacatcttg      300

tccaacgctt tgagacattt gcacgacaac ccagagatga agttcatctg ggctgaaatc      360

tcctacttcg ctagattcta ccacgatttg ggtgagaaca agaagttgca gatgaagtcc      420

atcgtcaaga acggtcagtt ggaattcgtc actggtggat gggtcatgcc agacgaggct      480

aactcccact ggagaaacgt tttgttgcag ttgaccgaag gtcaaacttg gttgaagcaa      540

ttcatgaacg tcactccaac tgcttcctgg gctatcgatc cattcggaca ctctccaact      600

atgccataca ttttgcagaa gtctggtttc aagaatatgt tgatccagag aacccactac      660

tccgttaaga aggagttggc tcaacagaga cagttggagt tcttgtggag acagatctgg      720

gacaacaaag gtgacactgc tttgttcacc cacatgatgc cattctactc ttacgacatt      780

cctcatacct gtggtccaga tccaaaggtt tgttgtcagt tcgatttcaa aagaatgggt      840

tccttcggtt tgtcttgtcc atggaaggtt ccacctagaa ctatctctga tcaaaatgtt      900

gctgctagat ccgatttgtt ggttgatcag tggaagaaga aggctgagtt gtacagaacc      960

aacgtcttgt tgattccatt gggtgacgac ttcagattca agcagaacac cgagtgggat     1020

gttcagagag tcaactacga aagattgttc gaacacatca actctcaggc tcacttcaat     1080

gtccaggctc agttcggtac tttgcaggaa tacttcgatg ctgttcacca ggctgaaaga     1140

gctggacaag ctgagttccc aaccttgtct ggtgacttct tcacttacgc tgatagatct     1200

gataactact ggtctggtta ctacacttcc agaccatacc ataagagaat ggacagagtc     1260

ttgatgcact acgttagagc tgctgaaatg ttgtccgctt ggcactcctg ggacggtatg     1320

gctagaatcg aggaaagatt ggagcaggct agaagagagt tgtccttgtt ccagcaccac     1380

gacggtatta ctggtactgc taaaactcac gttgtcgtcg actacgagca aagaatgcag     1440

gaagctttga aagcttgtca aatggtcatg caacagtctg tctacagatt gttgactaag     1500

ccatccatct actctccaga cttctccttc tcctacttca ctttggacga ctccagatgg     1560

ccaggttctg gtgttgagga ctctagaact accatcatct tgggtgagga tatcttgcca     1620

tccaagcatg ttgtcatgca caacaccttg ccacactgga gagagcagtt ggttgacttc     1680

tacgtctcct ctccattcgt ttctgttacc gacttggcta acaatccagt tgaggctcag     1740

gtttctccag tttggtcttg gcaccacgac actttgacta agactatcca cccacaaggt     1800

tccaccacca agtacagaat catcttcaag gctagagttc caccaatggg tttggctacc     1860

tacgttttga ccatctccga ttccaagcca gagcacacct cctacgcttc caatttgttg     1920

cttagaaaga acccaacttc cttgccattg ggtcaatacc cagaggatgt caagttcggt     1980

gatccaagag agatctcctt gagagttggt aacggtccaa ccttggcttt ctctgagcag     2040

ggtttgttga agtccattca gttgactcag gattctccac atgttccagt tcacttcaag     2100

ttcttgaagt acggtgttag atctcatggt gatagatctg gtgcttactt gttcttgcca     2160

aatggtccag cttctccagt cgagttgggt cagccagttg tcttggtcac taagggtaaa     2220

ttggagtctt ccgtttctgt tggtttgcca tctgtcgttc accagaccat catgagaggt     2280

ggtgctccag agattagaaa tttggtcgat attggttctt tggacaacac tgagatcgtc     2340

atgagattgg agactcatat cgactctggt gatatcttct acactgattt gaatggattg     2400

caattcatca agaggagaag attggacaag ttgccattgc aggctaacta ctacccaatt     2460

ccatctggta tgttcattga ggatgctaat accagattga ctttgttgac cggtcaacca     2520

ttgggtggat cttctttggc ttctggtgag ttggagatta tgcaagatag aagattggct     2580

tctgatgatg aaagaggttt gggtcagggt gttttggaca acaagccagt tttgcatatt     2640

tacagattgg tcttggagaa ggttaacaac tgtgtcagac catctaagtt gcatccagct     2700

ggttacttga cttctgctgc tcacaaagct tctcagtctt tgttggatcc attggacaag     2760

ttcatcttcg ctgaaaatga gtggatcggt gctcagggtc aattcggtgg tgatcatcca     2820

tctgctagag aggatttgga tgtctctgtc atgagaagat tgaccaagtc ttctgctaaa     2880

acccagagag ttggttacgt tttgcacaga accaatttga tgcaatgtgg tactccagag     2940

gagcatactc agaagttgga tgtctgtcac ttgttgccaa atgttgctag atgtgagaga     3000

actaccttga ctttcttgca gaatttggag cacttggatg gtatggttgc tccagaagtt     3060

tgtccaatgg aaaccgctgc ttacgtctct tctcactctt cttga                     3105


<210>  46
<211>  108
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encodes Mnn2 leader (53)

<400>  46
atgctgctta ccaaaaggtt ttcaaagctg ttcaagctga cgttcatagt tttgatattg       60

tgcgggctgt tcgtcattac aaacaaatac atggatgaga acacgtcg                   108


<210>  47
<211>  1729
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the PpHIS1 auxotrophic marker

<400>  47
caagttgcgt ccggtatacg taacgtctca cgatgatcaa agataatact taatcttcat       60

ggtctactga ataactcatt taaacaattg actaattgta cattatattg aacttatgca      120

tcctattaac gtaatcttct ggcttctctc tcagactcca tcagacacag aatatcgttc      180

tctctaactg gtcctttgac gtttctgaca atagttctag aggagtcgtc caaaaactca      240

actctgactt gggtgacacc accacgggat ccggttcttc cgaggacctt gatgaccttg      300

gctaatgtaa ctggagtttt agtatccatt ttaagatgtg tgtttctgta ggttctgggt      360

tggaaaaaaa ttttagacac cagaagagag gagtgaactg gtttgcgtgg gtttagactg      420

tgtaaggcac tactctgtcg aagttttaga taggggttac ccgctccgat gcatgggaag      480

cgattagccc ggctgttgcc cgtttggttt ttgaagggta attttcaata tctctgtttg      540

agtcatcaat ttcatattca aagattcaaa aacaaaatct ggtccaagga gcgcatttag      600

gattatggag ttggcgaatc acttgaacga tagactatta tttgctgttc ctaaagaggg      660

cagattgtat gagaaatgcg ttgaattact taggggatca gatattcagt ttcgaagatc      720

cagtagattg gatatagctt tgtgcactaa cctgcccctg gcattggttt tccttccagc      780

tgctgacatt cccacgtttg taggagaggg taaatgtgat ttgggtataa ctggtattga      840

ccaggttcag gaaagtgacg tagatgtcat acctttatta gacttgaatt tcggtaagtg      900

caagttgcag attcaagttc ccgagaatgg tgacttgaaa gaacctaaac agctaattgg      960

taaagaaatt gtttcctcct ttactagctt aaccaccagg tactttgaac aactggaagg     1020

agttaagcct ggtgagccac taaagacaaa aatcaaatat gttggagggt ctgttgaggc     1080

ctcttgtgcc ctaggagttg ccgatgctat tgtggatctt gttgagagtg gagaaaccat     1140

gaaagcggca gggctgatcg atattgaaac tgttctttct acttccgctt acctgatctc     1200

ttcgaagcat cctcaacacc cagaactgat ggatactatc aaggagagaa ttgaaggtgt     1260

actgactgct cagaagtatg tcttgtgtaa ttacaacgca cctagaggta accttcctca     1320

gctgctaaaa ctgactccag gcaagagagc tgctaccgtt tctccattag atgaagaaga     1380

ttgggtggga gtgtcctcga tggtagagaa gaaagatgtt ggaagaatca tggacgaatt     1440

aaagaaacaa ggtgccagtg acattcttgt ctttgagatc agtaattgta gagcatagat     1500

agaataatat tcaagaccaa cggcttctct tcggaagctc caagtagctt atagtgatga     1560

gtaccggcat atatttatag gcttaaaatt tcgagggttc actatattcg tttagtggga     1620

agagttcctt tcactcttgt tatctatatt gtcagcgtgg actgtttata actgtaccaa     1680

cttagtttct ttcaactcca ggttaagaga cataaatgtc ctttgatgc                 1729


<210>  48
<211>  1068
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encodes Rat GnT II  (TC)Codon-optimized

<400>  48
tccttggttt accaattgaa cttcgaccag atgttgagaa acgttgacaa ggacggtact       60

tggtctcctg gtgagttggt tttggttgtt caggttcaca acagaccaga gtacttgaga      120

ttgttgatcg actccttgag aaaggctcaa ggtatcagag aggttttggt tatcttctcc      180

cacgatttct ggtctgctga gatcaactcc ttgatctcct ccgttgactt ctgtccagtt      240

ttgcaggttt tcttcccatt ctccatccaa ttgtacccat ctgagttccc aggttctgat      300

ccaagagact gtccaagaga cttgaagaag aacgctgctt tgaagttggg ttgtatcaac      360

gctgaatacc cagattcttt cggtcactac agagaggcta agttctccca aactaagcat      420

cattggtggt ggaagttgca ctttgtttgg gagagagtta aggttttgca ggactacact      480

ggattgatct tgttcttgga ggaggatcat tacttggctc cagacttcta ccacgttttc      540

aagaagatgt ggaagttgaa gcaacaagag tgtccaggtt gtgacgtttt gtccttggga      600

acttacacta ctatcagatc cttctacggt atcgctgaca aggttgacgt taagacttgg      660

aagtccactg aacacaacat gggattggct ttgactagag atgcttacca gaagttgatc      720

gagtgtactg acactttctg tacttacgac gactacaact gggactggac tttgcagtac      780

ttgactttgg cttgtttgcc aaaagtttgg aaggttttgg ttccacaggc tccaagaatt      840

ttccacgctg gtgactgtgg aatgcaccac aagaaaactt gtagaccatc cactcagtcc      900

gctcaaattg agtccttgtt gaacaacaac aagcagtact tgttcccaga gactttggtt      960

atcggagaga agtttccaat ggctgctatt tccccaccaa gaaagaatgg tggatggggt     1020

gatattagag accacgagtt gtgtaaatcc tacagaagat tgcagtag                  1068


<210>  49
<211>  300
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encodes Mnn2 leader (54)

<400>  49
atgctgctta ccaaaaggtt ttcaaagctg ttcaagctga cgttcatagt tttgatattg       60

tgcgggctgt tcgtcattac aaacaaatac atggatgaga acacgtcggt caaggagtac      120

aaggagtact tagacagata tgtccagagt tactccaata agtattcatc ttcctcagac      180

gccgccagcg ctgacgattc aaccccattg agggacaatg atgaggcagg caatgaaaag      240

ttgaaaagct tctacaacaa cgttttcaac tttctaatgg ttgattcgcc cgggcgcgcc      300


<210>  50
<211>  1373
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the 5'-Region used for knock out of PpARG1

<400>  50
gatctggcct tccctgaatt tttacgtcca gctatacgat ccgttgtgac tgtatttcct       60

gaaatgaagt ttcaacctaa agttttggtt gtacttgctc cacctaccac ggaaactaat      120

atcgaaacca atgaaaaagt agaactggaa tcgtcaatcg aaattcgcaa ccaagtggaa      180

cccaaagact tgaatctttc taaagtctat tctagtgaca ctaatggcaa cagaagattt      240

gagctgactt ttcaaatgaa tctcaataat gcaatatcaa catcagacaa tcaatgggct      300

ttgtctagtg acacaggatc aattatagta gtgtcttctg caggaagaat aacttccccg      360

atcctagaag tcggggcatc cgtctgtgtc ttaagatcgt acaacgaaca ccttttggca      420

ataacttgtg aaggaacatg cttttcatgg aatttaaaga agcaagaatg tgttctaaac      480

agcatttcat tagcacctat agtcaattca cacatgctag ttaagaaagt tggagatgca      540

aggaactatt ctattgtatc tgccgaagga gacaacaatc cgttacccca gattctagac      600

tgcgaacttt ccaaaaatgg cgctccaatt gtggctctta gcacgaaaga catctactct      660

tattcaaaga aaatgaaatg ctggatccat ttgattgatt cgaaatactt tgaattgttg      720

ggtgctgaca atgcactgtt tgagtgtgtg gaagcgctag aaggtccaat tggaatgcta      780

attcatagat tggtagatga gttcttccat gaaaacactg ccggtaaaaa actcaaactt      840

tacaacaagc gagtactgga ggacctttca aattcacttg aagaactagg tgaaaatgcg      900

tctcaattaa gagagaaact tgacaaactc tatggtgatg aggttgaggc ttcttgacct      960

cttctctcta tctgcgtttc tttttttttt tttttttttt tttttttcag ttgagccaga     1020

ccgcgctaaa cgcataccaa ttgccaaatc aggcaattgt gagacagtgg taaaaaagat     1080

gcctgcaaag ttagattcac acagtaagag agatcctact cataaatgag gcgcttattt     1140

agtagctagt gatagccact gcggttctgc tttatgctat ttgttgtatg ccttactatc     1200

tttgtttggc tcctttttct tgacgttttc cgttggaggg actccctatt ctgagtcatg     1260

agccgcacag attatcgccc aaaattgaca aaatcttctg gcgaaaaaag tataaaagga     1320

gaaaaaagct cacccttttc cagcgtagaa agtatatatc agtcattgaa gac            1373


<210>  51
<211>  1470
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the 3'-Region used for knock out of PpARG1

<400>  51
gggactttaa ctcaagtaaa aggatagttg tacaattata tatacgaaga ataaatcatt       60

acaaaaagta ttcgtttctt tgattcttaa caggattcat tttctgggtg tcatcaggta      120

cagcgctgaa tatcttgaag ttaacatcga gctcatcatc gacgttcatc acactagcca      180

cgtttccgca acggtagcaa taattaggag cggaccacac agtgacgaca tctttctctt      240

tgaaatggta tctgaagcct tccatgacca attgatgggc tctagcgatg agttgcaagt      300

tattaatgtg gttgaactca cgtgctactc gagcaccgaa taaccagcca gctccacgag      360

gagaaacagc ccaactgtcg acttcatctg ggtcagacca aaccaagtca caaaatcctc      420

cttcatgagg gacctcttgc gctcggctga gaactctgat ttgatctaac atgcgaatat      480

cgggagagag accaccatgg atacataata ttttaccatc aatgatggca ctaagggtta      540

aaaagtcgaa cacctggcaa cagtacttcc agacagtggt ggaaccatat ttattgagac      600

attcctcata aaatccataa acctgagtga tctgtctgga ttcatgattt ccccttacca      660

atgtgatatg ttgaggaaac ttaattttta aaatcatgag taacgtgaac gtctccaacg      720

agaaatagcc tctatccaca tagtctccta ggaagatata gttctgtttt attccattag      780

aggaggatcc gggaaaccca ccactaatct tgaaaagttc cagtagatcg tgaaattggc      840

cgtgaatatc tccgcatact gtcactggac tctgcactgg ctgtatattg gattcctcca      900

tcagcaaatc cttcacccgt tcgcaaagat gcttcatatc attttcactt aaagccttgc      960

agcttttgac ttcttcaaac cactgatctg gtcctctttc tggcatgatt aaggtctata     1020

atatttctga gctgagatgt aaaaaaaaat aataaaaatg gggagtgaaa aagtgtgtag     1080

cttttaggag tttgggattg ataccccaaa atgatcttta tgagaattaa aaggtagata     1140

cgcttttaat aagaacacct atctatagta ctttgtggtc ttgagtaatt gagatgttca     1200

gcttctgagg tttgccgtta ttctgggata gtagtgcgcg accaaacaac ccgccaggca     1260

aagtgtgttg tgctcgaaga cgattgccag aagagtaagt ccgtcctgcc tcagatgtta     1320

cacactttct tccctagaca gtcgatgcat catcggattt aaacctgaaa ctttgatgcc     1380

atgatacgcc tagtcacgtc gactgagatt ttagataagc cccgatccct ttagtacatt     1440

cctgttatcc atggatggaa tggcctgata                                      1470


<210>  52
<211>  1666
<212>  DNA
<213>  Artificial

<220>
<223>  HYGR resistance cassette

<400>  52
gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga       60

ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt      120

acatttagcc catacatccc catgtataat catttgcatc catacatttt gatggccgca      180

cggcgcgaag caaaaattac ggctcctcgc tgcggacctg cgagcaggga aacgctcccc      240

tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg      300

atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt      360

ctcacatcac atccgaacat aaacaaccat gggtaaaaag cctgaactca ccgcgacgtc      420

tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga      480

gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt      540

aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc      600

cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg      660

catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc      720

tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac      780

gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt      840

catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt      900

cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga      960

agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg     1020

cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc     1080

caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga     1140

gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg     1200

tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca     1260

gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc     1320

ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa     1380

ccgacgcccc agcactcgtc cgagggcaaa ggaataatca gtactgacaa taaaaagatt     1440

cttgttttca agaacttgtc atttgtatag tttttttata ttgtagttgt tctattttaa     1500

tcaaatgtta gcgtgattta tatttttttt cgcctcgaca tcatctgccc agatgcgaag     1560

ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt cgctatactg     1620

ctgtcgattc gatactaacg ccgccatcca gtgtcgaaaa cgagct                    1666


<210>  53
<211>  388
<212>  DNA
<213>  Artificial

<220>
<223>  Ashbya gossypii TEF1 promoter

<400>  53
gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga       60

ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt      120

acatttagcc catacatccc catgtataat catttgcatc catacatttt gatggccgca      180

cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc      240

tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg      300

atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt      360

ctcacatcac atccgaacat aaacaacc                                         388


<210>  54
<211>  247
<212>  DNA
<213>  Artificial

<220>
<223>  Ashbya gossypii TEF1 termination sequence

<400>  54
taatcagtac tgacaataaa aagattcttg ttttcaagaa cttgtcattt gtatagtttt       60

tttatattgt agttgttcta ttttaatcaa atgttagcgt gatttatatt ttttttcgcc      120

tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa agtaatatca tgcgtcaatc      180

gtatgtgaat gctggtcgct atactgctgt cgattcgata ctaacgccgc catccagtgt      240

cgaaaac                                                                247


<210>  55
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  Primer SH512

<400>  55
gagacgatag acggtgagga ttcagaagat cctg                                   34


<210>  56
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Primer SH97

<400>  56
ggggagaagg taccgaagcc ggag                                              24


<210>  57
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  Primer SH515

<400>  57
ccatacacca gatgtatctc aaaaatgtca ac                                     32


<210>  58
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Primer SH379

<400>  58
catgcccctg agctgcgcac gtcaag                                            26


<210>  59
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  Primer SH520

<400>  59
caacttggct ctgggctcgt ttgtattg                                          28


<210>  60
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  Primer SH521

<400>  60
ggtgtcttca gggaagttct gagctatg                                          28


<210>  61
<211>  934
<212>  DNA
<213>  Artificial

<220>
<223>  Pp AOX1 promoter

<400>  61
aacatccaaa gacgaaaggt tgaatgaaac ctttttgcca tccgacatcc acaggtccat       60

tctcacacat aagtgccaaa cgcaacagga ggggatacac tagcagcaga ccgttgcaaa      120

cgcaggacct ccactcctct tctcctcaac acccactttt gccatcgaaa aaccagccca      180

gttattgggc ttgattggag ctcgctcatt ccaattcctt ctattaggct actaacacca      240

tgactttatt agcctgtcta tcctggcccc cctggcgagg ttcatgtttg tttatttccg      300

aatgcaacaa gctccgcatt acacccgaac atcactccag atgagggctt tctgagtgtg      360

gggtcaaata gtttcatgtt ccccaaatgg cccaaaactg acagtttaaa cgctgtcttg      420

gaacctaata tgacaaaagc gtgatctcat ccaagatgaa ctaagtttgg ttcgttgaaa      480

tgctaacggc cagttggtca aaaagaaact tccaaaagtc ggcataccgt ttgtcttgtt      540

tggtattgat tgacgaatgc tcaaaaataa tctcattaat gcttagcgca gtctctctat      600

cgcttctgaa ccccggtgca cctgtgccga aacgcaaatg gggaaacacc cgctttttgg      660

atgattatgc attgtctcca cattgtatgc ttccaagatt ctggtgggaa tactgctgat      720

agcctaacgt tcatgatcaa aatttaactg ttctaacccc tacttgacag caatatataa      780

acagaaggaa gctgccctgt cttaaacctt tttttttatc atcattatta gcttactttc      840

ataattgcga ctggttccaa ttgacaagct tttgatttta acgactttta acgacaactt      900

gagaagatca aaaaacaact aattattcga aacg                                  934


<210>  62
<211>  375
<212>  DNA
<213>  Artificial

<220>
<223>  Sequence of the Sh ble ORF (Zeocin resistance marker)

<400>  62
atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc       60

gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt      120

gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac      180

aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag      240

gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag      300

ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc      360

gaggagcagg actga                                                       375


<210>  63
<211>  427
<212>  DNA
<213>  Artificial

<220>
<223>  ScTEF1 promoter

<400>  63
gatcccccac acaccatagc ttcaaaatgt ttctactcct tttttactct tccagatttt       60

ctcggactcc gcgcatcgcc gtaccacttc aaaacaccca agcacagcat actaaatttc      120

ccctctttct tcctctaggg tgtcgttaat tacccgtact aaaggtttgg aaaagaaaaa      180

agagaccgcc tcgtttcttt ttcttcgtcg aaaaaggcaa taaaaatttt tatcacgttt      240

ctttttcttg aaaatttttt tttttgattt ttttctcttt cgatgacctc ccattgatat      300

ttaagttaat aaacggtctt caatttctca agtttcagtt tcatttttct tgttctatta      360

caactttttt tacttcttgc tcattagaaa gaaagcatag caatctaatc taagttttaa      420

ttacaaa                                                                427


<210>  64
<211>  1936
<212>  DNA
<213>  Pichia pastoris

<400>  64
ggccagccca tcaccatgaa tgcttaaaac gccaactcct tccatctcat tttcgtacca       60

gattatgact cttaggcggg gagaatcccg tccagcatag cgaacatttc tttttttttt      120

ttttttcgtt tcgcatctct ctatcgcatt cagaaaaaaa tacatataat tcttccagtt      180

tccgtcattc attacgttta aaactacgaa agttttagct ctcttttgtt tttgtttcct      240

agattcgaaa tattttcttt attgagttta atttgtgtgg cagacaatgg ttagatcttt      300

caccatcaaa gtgcctgctt cctcagcaaa tataggaccg gggtttgacg ttctgggaat      360

tggtctcaac ctttacttgg aactacaagt caccattgat cccaaaattg atacctcaag      420

cgatccagaa aatgtgttat tgtcgtatga aggtgagggg gctgatgagg tgtcattgaa      480

aagtgacgaa aacttgatta cgcgcacagc tctctatgtt ctacgttgtg acgacgtcag      540

gactttccct aagggaacca agattcacgt cattaaccct attcctctag gaagaggctt      600

gggatcttcg ggtgctgcag ttgtcgccgg tgcattgctc ggaaattcca tcggacagct      660

tggatactcc aaacaacgtt tactggatta ctgtttgatg atagaacgtc atccagataa      720

catcaccgca gctatggtgg gtggtttcgt tggatcttat cttagagatc tttcaccaga      780

agacacccag agaaaagaga ttccattagc agaagtcctg ccagaacctc aaggtggtat      840

taacaccggt ctcaacccac cagtgcctcc aaaaaacatt gggcaccaca tcaaatacgg      900

ctgggcaaaa gagatcaaat gtattgccat tattccagac tttgaagtat caaccgcttc      960

atctagaggc gttcttccaa ccacttacga gagacatgac attattttca acctgcaaag     1020

gatagccgtt cttaccactg ccctgacaca atctccacca gatccaagct tgatataccc     1080

agctatgcag gacaggattc accaacctta caggaaaact ttgatccacg gactgactga     1140

aatactgtct tcattcaccc cagaattaca caaaggtttg ttgggaatct gtctttccgg     1200

tgctgggccc acaatattag ccctcgcaac tgaaaacttc gatcagattg ctaaggacat     1260

cattgccaga tttgctgtcg aagacatcac ctgtagttgg aaactcttga ccccagctct     1320

tgaaggttct gttgttgagg agcttgctta atagaaatta gaacatcctc tttagattat     1380

gataatacgt ttttaacttt tcccctaact gtagtgatgg tatctgaccc tcttagacct     1440

taggttggac cttctcgaat ttcctgcctc tatcaaaaat ccgaccctcg acatcgttta     1500

cgtactttgc aaccaattaa ctagtaccgg cagacgttca gtgatcatgg ctctctatac     1560

aaataccctg ataacgtttg cattcctgac agtcggagga tgtacgtgct tattttcttg     1620

ctagtcccaa atgttttgag attgctccaa tcgttttttc aacaatacta actgccaaca     1680

aatagatctt ttattcaacg gaaatgggga acaattcaac gtgggtgact ttttggagac     1740

tacatctccc tatatgtggg caaatctggg tatagcaagt tgcattggat tctcggtcat     1800

tggtgctgca tggggaattt tcataacagg ttcttcgatc atcggtgcag gtgtcaaagc     1860

tcccagaatc acaacaaaaa atttaatctc catcattttc tgtgaggtgg tggctattta     1920

tgggcttatt atggcc                                                     1936


<210>  65
<211>  672
<212>  DNA
<213>  Artificial

<220>
<223>  PpTRP1 5' region and ORF

<400>  65
gcggaaacgg cagtaaacaa tggagcttca ttagtgggtg ttattatggt ccctggccgg       60

gaacgaacgg tgaaacaaga ggttgcgagg gaaatttcgc agatggtgcg ggaaaagaga      120

atttcaaagg gctcaaaata cttggattcc agacaactga ggaaagagtg ggacgactgt      180

cctctggaag actggtttga gtacaacgtg aaagaaataa acagcagtgg tccattttta      240

gttggagttt ttcgtaatca aagtatagat gaaatccagc aagctatcca cactcatggt      300

ttggatttcg tccaactaca tgggtctgag gattttgatt cgtatatacg caatatccca      360

gttcctgtga ttaccagata cacagataat gccgtcgatg gtcttaccgg agaagacctc      420

gctataaata gggccctggt gctactggac agcgagcaag gaggtgaagg aaaaaccatc      480

gattgggctc gtgcacaaaa atttggagaa cgtagaggaa aatatttact agccggaggt      540

ttgacacctg ataatgttgc tcatgctcga tctcatactg gctgtattgg tgttgacgtc      600

tctggtgggg tagaaacaaa tgcctcaaaa gatatggaca agatcacaca atttatcaga      660

aacgctacat aa                                                          672


<210>  66
<211>  834
<212>  DNA
<213>  Artificial

<220>
<223>  PpTRP1 3' region

<400>  66
aagtcaatta aatacacgct tgaaaggaca ttacatagct ttcgatttaa gcagaaccag       60

aaatgtagaa ccacttgtca atagattggt caatcttagc aggagcggct gggctagcag      120

ttggaacagc agaggttgct gaaggtgaga aggatggagt ggattgcaaa gtggtgttgg      180

ttaagtcaat ctcaccaggg ctggttttgc caaaaatcaa cttctcccag gcttcacggc      240

attcttgaat gacctcttct gcatacttct tgttcttgca ttcaccagag aaagcaaact      300

ggttctcagg ttttccatca gggatcttgt aaattctgaa ccattcgttg gtagctctca      360

acaagcccgg catgtgcttt tcaacatcct cgatgtcatt gagcttagga gccaatgggt      420

cgttgatgtc gatgacgatg accttccagt cagtctctcc ctcatccaac aaagccataa      480

caccgaggac cttgacttgc ttgacctgtc cagtgtaacc tacggcttca ccaatttcgc      540

aaacgtccaa tggatcattg tcacccttgg ccttggtctc tggatgagtg acgttagggt      600

cttcccatgt ctgagggaag gcaccgtagt tgtgaatgta tccgtggtga gggaaacagt      660

tacgaacgaa acgaagtttt cccttctttg tgtcctgaag aattgggttc agtttctcct      720

ccttggaaat ctccaacttg gcgttggtcc aacgggggac ttcaacaacc atgttgagaa      780

ccttcttgga ttcgtcagca taaagtggga tgtcgtggaa aggagatacg actt            834


<210>  67
<211>  1400
<212>  DNA
<213>  Artificial

<220>
<223>  NatR expression cassette

<400>  67
gagttaggtt cacatacgat ttaggtgaca ctatagaacg cggccgccag ctgaagcttc       60

gtacgctgca ggtcgacgga tccccgggtt aattaaggcg cgccagatct gtttagcttg      120

cctcgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc ctccttgaca      180

gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac      240

atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa      300

attacggctc ctcgctgcag acctgcgagc agggaaacgc tcccctcaca gacgcgttga      360

attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg ccactgaggt      420

tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac atcacatccg      480

aacataaaca accatgggta ccactcttga cgacacggct taccggtacc gcaccagtgt      540

cccgggggac gccgaggcca tcgaggcact ggatgggtcc ttcaccaccg acaccgtctt      600

ccgcgtcacc gccaccgggg acggcttcac cctgcgggag gtgccggtgg acccgcccct      660

gaccaaggtg ttccccgacg acgaatcgga cgacgaatcg gacgacgggg aggacggcga      720

cccggactcc cggacgttcg tcgcgtacgg ggacgacggc gacctggcgg gcttcgtggt      780

catctcgtac tcggcgtgga accgccggct gaccgtcgag gacatcgagg tcgccccgga      840

gcaccggggg cacggggtcg ggcgcgcgtt gatggggctc gcgacggagt tcgccggcga      900

gcggggcgcc gggcacctct ggctggaggt caccaacgtc aacgcaccgg cgatccacgc      960

gtaccggcgg atggggttca ccctctgcgg cctggacacc gccctgtacg acggcaccgc     1020

ctcggacggc gagcggcagg cgctctacat gagcatgccc tgcccctaat cagtactgac     1080

aataaaaaga ttcttgtttt caagaacttg tcatttgtat agttttttta tattgtagtt     1140

gttctatttt aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga catcatctgc     1200

ccagatgcga agttaagtgc gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg     1260

gtcgctatac tgctgtcgat tcgatactaa cgccgccatc cagtgtcgaa aacgagctcg     1320

aattcatcga tgatatcaga tccactagtg gcctatgcgg ccgcggatct gccggtctcc     1380

ctatagtgag tcgtattcac                                                 1400


<210>  68
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Primer SH1406

<400>  68
gtttcgcgtt ctcacttaga tggag                                             25


<210>  69
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Primer SH1420

<400>  69
ccatttctcc gtcaatccga ttctcgc                                           27


<210>  70
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  Primer SH1407

<400>  70
ccactcgcca gatcggagct gcaaacactc                                        30


<210>  71
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Primer SH1421

<400>  71
ccgccctgta cgacggcacc gcctc                                             25


<210>  72
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  Primer SH1417

<400>  72
cgaacctttt ccccaacata tttggcaaac g                                      31


<210>  73
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  Primer SH1418

<400>  73
gcaaggtgat ggttcaaatc tccagctcca c                                      31


<210>  74
<211>  2004
<212>  DNA
<213>  Artificial

<220>
<223>  Pichia pastoris ATT1 5' region in pGLY5933

<400>  74
ggccgggact acatgaggcc gattcttcaa gccagggaaa ttaattgctt gaaccggaaa       60

atcattaagg caggcaacga aaaatccaac tccttggttg aattgactca aaagtttatc      120

ttacggagaa aagctaaaga catcaatacg aatttccttc cgccaaaaac tgaactgata      180

ctgatggttc caatgactga attacaacag gagctataca aggatataat tgaaactaac      240

caagccaagc ttggcttgat caacgacaga aacttttttc ttcaaaaaat tttgattctt      300

cgtaaaatat gcaattcacc ctccctgctg aaagacgaac ctgattttgc cagatacaat      360

ctcggcaata gattcaatag cggtaagatc aagctaacag tactgctttt acgaaagctg      420

tttgaaacca ccaatgagaa gtgtgtgatt gtttcaaact tcactaaaac tttggacgta      480

cttcagctaa tcatagagca caacaattgg aaataccacc gactagatgg ttcgagtaaa      540

ggacgggaca aaatcgtacg agattttaac gagtcgcctc aaaaagatcg attcatcatg      600

ttgctttctt ccaaggcagg gggagtgggg ctcaacttaa ttggagcctc acgcttaatt      660

ctttttgata acgactggaa tcccagtgtt gacattcaag caatggctag agtgcatcga      720

gacgggcaga aaaggcacac ctttatctat cgtttgtata cgaaaggcac aattgacgaa      780

aagatcctac aaaggcaatt gatgaaacaa aatctgagcg acaaattcct ggatgataat      840

gatagcagca aggatgatgt gtttaacgac tacgatctca aagatttgtt tactgtagat      900

cttgacacga attgtagtac acacgatttg atggaatgtt tatgtaatgg gcggctgaga      960

gatccgactc ccgtcttgga agcagaagaa tgcaagacaa aaccgttgga ggccgttgac     1020

gacacggatg atggttggat gtcagctctg gatttcaaac agttatcaca aaaagaggag     1080

acaggtgctg tgtcaacaat gcgtcaatgt ctgctcggat atcaacacat tgatccaaag     1140

attttggaac caacagaacc tgtaggggac gatttggtat tggcaaacat cctcgcggag     1200

tcctcaggct tggctaaatc tgcattgtca tctgaaaaga aacccaagaa accagtggtg     1260

aactttatct ttgtgtcagg ccaagactaa gctggaagaa cggaacttta atcgaaggaa     1320

aaattaaatg tcaaagtggg tcgatcagga gataatccat gcttcacgtg atttttctta     1380

ataaacgccg gaaaaacttt cttttttgtg accaaaatta tccgatctga aaaaaaatta     1440

cgcatgcgtg aagtaggatg agagacttac tgttgaactt tgtgagacga ggggaaaagg     1500

aatatcctga tcgtaaacaa aaaagttttc cagcccaatc gggaacatct gcgaagtgtt     1560

ggaattcaac ccctctttcg aaaatgttcc attttaccca aaattattgt tattaaataa     1620

tacatgtgtt actagcaaag tctgcgcttt ccatgtctca gattcggcag ataacaaagt     1680

tgacacgttc ttgcgagata cgcatgaatc ttttggctgc tttttgtgaa agagaaatgg     1740

tgccatatat tgcagacgcc cctgaaagat tagtgtgcgg ctgagtcttt tttttttctc     1800

aaccagcttt ttctttttat tgggtaccat cgcgcacgca ggactcatgc tccattagac     1860

ttctgaacca cctgacttaa tattcatgga cggacgcttt tatccttaaa ttgttcatcc     1920

attcctcaat ttttccgttt gccctccctg tactattaaa ttacaaaagc tgatcttttt     1980

caagtgtttc tctttgaatc gctc                                            2004


<210>  75
<211>  1854
<212>  DNA
<213>  Artificial

<220>
<223>  Pichia pastoris ATT1 3' region in pGLY5933

<400>  75
ggaccctgaa gacgaagaca tgtctgcctt agagtttacc gcagttcgat tccccaactt       60

ttcagctacg acaacagccc cgcctcctac tccagtcaat tgcaacagtc ctgaaaacat      120

caagacctcc actgtggacg attttttgaa agctactcaa gatccaaata acaaagagat      180

actcaacgac atttacagtt tgatttttga tgactccatg gatcctatga gcttcggaag      240

tatggaacca agaaacgatt tggaagttcc ggacactata atggattaat ttgcagcggg      300

cctgtttgta tagtctttga ttgtgtataa tagaattact acgcgtatat cccgatctgg      360

aagtaacatg gaagtttccc attttcgcgc agtctcctac tcgtatcctc cccacccctt      420

accgatgacg caaaaggtca ctagataagc atagcatagt ttcatccctt gctctttcct      480

tgtaccaaca gatcatggct gggaatctca aggatattct atccttgtcg aggaagacag      540

caaggaatct gaagcaggct ctggatgagc ttgcggagca ggtgatcaac caccaacgga      600

gacgaccagc tctggtccga gttcctatca acaacaacct taggcgcaag agccagcagt      660

cctttttgaa tcgcaggtca ttccatcttt ggaccagcaa gtacaaccca tacttttgga      720

ggggaggcag aagcaacgtt ctggaccagc ttaaccgtga agctttaagg tacagatcgt      780

cttttgcgaa acccggattt tatccaagtg ggctgtatca gtcaactttc cctcaaagag      840

gtagtaggat gttttccacc tgcgcctact catgtcagca ggaggcagtc aaaaacttga      900

cttccgctgt tcgtgctttg ttacaaagtg gtgctaattt cggcagtcaa atgaaacaaa      960

tgaaacactg ttcgcaaaag aagaagcact tctctaaatt ttctaagagg cttacttctt     1020

ccactgccgc tgggtctggc aagaatgctg aacaagctcc ttctggtttg gccgaaggat     1080

ccgctgttgt ttttagcctt gaacgtcaaa gtcacaatac tgagttggaa ggaatcttgg     1140

atcaagaaac ttcttccatt ctcgaggaag aaatggttca acatgagcgt cacctggcta     1200

ttattagaga agaaatccag agaattagtg agaatctagg atcattacca ttaatcatgt     1260

ctggtcacaa gattgaggta tttttcccca attgtgacac tgttaaatgt gagcaactga     1320

tgagagattt ggctattacg aaaggggttg tgaggcgtca tgattctact gctgagcatt     1380

caagctccag gtcatttgtt ccagaagatt gcttgtattc ctcagggtca agttcaccga     1440

atcctttatc ctcaacttct tcgaaatcat ttgatagagt ctcattggac tacatttcct     1500

ctcggtctac atctgatcaa accactggtt ctgagtacac atctctgtct caacaatatc     1560

acctggttag caattacaac cctgtactat cctcagcccc gggttcttcg agggtcttgg     1620

agctgaatac tcccgagtcc actatggaag gcagtacaga tctggagtat ttaacgcgag     1680

acgatgtgtt gctgttaaat gtctaatcta gacctatcct tcattctata tagcttagtt     1740

gagttttacg taagccctag tttttgttaa ttcttatcga tttatggtta gtgtaccact     1800

caactcacga tgatatatcc caggagctgt ttgtgcatta taactaccaa tcct           1854


<210>  76
<211>  987
<212>  DNA
<213>  Pichia pastoris

<400>  76
atggctgaca aaggatcggt agcggctaaa tcgcttacca actctgcacc cttatccatc       60

ttttcttact gtgctgcatc aattctgatg acagttacca ataagtatgc cgtgtccggt      120

gtcgatttca actttaactt ctttttgctt gccgttcagg gaatcgtttg tattaccttg      180

attagctcgt tgaagcaatt gaatgttatc acctttagag agttcaacaa ggttgaagca      240

aagaaatggt tcccaatcgc cgtgctgtta gttgtcatga tttatacctc ctccaaggct      300

ctacagtatc tgagcattcc aatttacacg atattcaaaa acttgaccat tatccttatt      360

gcttatggtg aagtcatctg gttcggaggc cgtgtgacca acttggctct gggctcgttt      420

gtattgatgg tgctctcctc tgcagtggct tcttatggtg attctaatgt tgacactggt      480

aaactcaatt ttaacattgg ctatttctgg atgttcacca actgtttctc ctctgccgca      540

tttgtgttgt tcatgagaaa gagaataaag ttgaccaact tcaaagactt tgacaccatg      600

tattacaaca accttctctc cattccaatt ttgctctttg catctttgac tactgaagac      660

tggtccgcta aaaacatagc tcagaacttc cctgaagaca ccaaatacgc tgtcatcgct      720

tccatgatta tttcaggaat gtctgccgtg ggtatctcat acacatctgc atggtgtgtc      780

cgtgtgacat cttccacgac atactcgatg gttggtgctt tgaacaagct tccaattgcc      840

ctgtctggtt tgctattttt caaggctcct atcaacttct attctatcag ctctatcttt      900

attggttttg ccgctggtct agtctatgcc attgccaagc agaagcaaaa gaaggaagac      960

gagttgcagt taccaactga caagagc                                          987


<210>  77
<211>  329
<212>  PRT
<213>  Pichia pastoris

<400>  77

Met Ala Asp Lys Gly Ser Val Ala Ala Lys Ser Leu Thr Asn Ser Ala 
1               5                   10                  15      


Pro Leu Ser Ile Phe Ser Tyr Cys Ala Ala Ser Ile Leu Met Thr Val 
            20                  25                  30          


Thr Asn Lys Tyr Ala Val Ser Gly Val Asp Phe Asn Phe Asn Phe Phe 
        35                  40                  45              


Leu Leu Ala Val Gln Gly Ile Val Cys Ile Thr Leu Ile Ser Ser Leu 
    50                  55                  60                  


Lys Gln Leu Asn Val Ile Thr Phe Arg Glu Phe Asn Lys Val Glu Ala 
65                  70                  75                  80  


Lys Lys Trp Phe Pro Ile Ala Val Leu Leu Val Val Met Ile Tyr Thr 
                85                  90                  95      


Ser Ser Lys Ala Leu Gln Tyr Leu Ser Ile Pro Ile Tyr Thr Ile Phe 
            100                 105                 110         


Lys Asn Leu Thr Ile Ile Leu Ile Ala Tyr Gly Glu Val Ile Trp Phe 
        115                 120                 125             


Gly Gly Arg Val Thr Asn Leu Ala Leu Gly Ser Phe Val Leu Met Val 
    130                 135                 140                 


Leu Ser Ser Ala Val Ala Ser Tyr Gly Asp Ser Asn Val Asp Thr Gly 
145                 150                 155                 160 


Lys Leu Asn Phe Asn Ile Gly Tyr Phe Trp Met Phe Thr Asn Cys Phe 
                165                 170                 175     


Ser Ser Ala Ala Phe Val Leu Phe Met Arg Lys Arg Ile Lys Leu Thr 
            180                 185                 190         


Asn Phe Lys Asp Phe Asp Thr Met Tyr Tyr Asn Asn Leu Leu Ser Ile 
        195                 200                 205             


Pro Ile Leu Leu Phe Ala Ser Leu Thr Thr Glu Asp Trp Ser Ala Lys 
    210                 215                 220                 


Asn Ile Ala Gln Asn Phe Pro Glu Asp Thr Lys Tyr Ala Val Ile Ala 
225                 230                 235                 240 


Ser Met Ile Ile Ser Gly Met Ser Ala Val Gly Ile Ser Tyr Thr Ser 
                245                 250                 255     


Ala Trp Cys Val Arg Val Thr Ser Ser Thr Thr Tyr Ser Met Val Gly 
            260                 265                 270         


Ala Leu Asn Lys Leu Pro Ile Ala Leu Ser Gly Leu Leu Phe Phe Lys 
        275                 280                 285             


Ala Pro Ile Asn Phe Tyr Ser Ile Ser Ser Ile Phe Ile Gly Phe Ala 
    290                 295                 300                 


Ala Gly Leu Val Tyr Ala Ile Ala Lys Gln Lys Gln Lys Lys Glu Asp 
305                 310                 315                 320 


Glu Leu Gln Leu Pro Thr Asp Lys Ser 
                325                 


