                         SEQUENCE LISTING

<110>  UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF 
       HIGHER EDUCATION
       Ross, Ted M.
       Crevar, Corey J.
       Giles, Brendan M.
 
<120>  COMPUTATIONALLY OPTIMIZED BROADLY REACTIVE ANTIGENS FOR HUMAN AND
        AVIAN H5N1 INFLUENZA

<130>  8123-88295-02

<150>  US 61/597,998
<151>  2012-02-13

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  566
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  1

Met Glu Lys Ile Val Leu Leu Leu Ala Ile Val Ser Leu Val Lys Ser 
1               5                   10                  15      


Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val 
            20                  25                  30          


Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile 
        35                  40                  45              


Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys 
    50                  55                  60                  


Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn 
65                  70                  75                  80  


Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val 
                85                  90                  95      


Glu Lys Ala Ser Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asp Phe Asn 
            100                 105                 110         


Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu 
        115                 120                 125             


Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asn His Glu Ala Ser 
    130                 135                 140                 


Ser Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly Lys Ser Ser Phe Phe 
145                 150                 155                 160 


Arg Asn Val Val Trp Leu Ile Lys Lys Asn Ser Ala Tyr Pro Thr Ile 
                165                 170                 175     


Lys Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp 
            180                 185                 190         


Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Lys Leu Tyr Gln 
        195                 200                 205             


Asn Pro Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg 
    210                 215                 220                 


Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly 
225                 230                 235                 240 


Arg Met Glu Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn 
                245                 250                 255     


Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile 
            260                 265                 270         


Val Lys Lys Gly Asp Ser Ala Ile Met Lys Ser Glu Leu Glu Tyr Gly 
        275                 280                 285             


Asn Cys Asn Thr Lys Cys Gln Thr Pro Met Gly Ala Ile Asn Ser Ser 
    290                 295                 300                 


Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys 
305                 310                 315                 320 


Tyr Val Lys Ser Asn Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Ser 
                325                 330                 335     


Pro Gln Arg Glu Arg Arg Arg Lys Lys Arg Gly Leu Phe Gly Ala Ile 
            340                 345                 350         


Ala Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr 
        355                 360                 365             


Gly Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys 
    370                 375                 380                 


Glu Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser 
385                 390                 395                 400 


Ile Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe 
                405                 410                 415     


Asn Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp 
            420                 425                 430         


Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met 
        435                 440                 445             


Glu Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu 
    450                 455                 460                 


Tyr Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly 
465                 470                 475                 480 


Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu 
                485                 490                 495     


Ser Val Arg Asn Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala 
            500                 505                 510         


Arg Leu Lys Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly 
        515                 520                 525             


Thr Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala 
    530                 535                 540                 


Leu Ala Ile Met Val Ala Gly Leu Ser Leu Trp Met Cys Ser Asn Gly 
545                 550                 555                 560 


Ser Leu Gln Cys Arg Ile 
                565     


<210>  2
<211>  1736
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  2
atggaaaaga tcgtgctgct gctggccatc gtgtccctgg tgaagagcga ccagatttgc       60

attggctacc acgccaacaa tagcacagag caggtggaca ccatcatgga gaaaaacgtg      120

accgtgaccc acgcccagga catcctggag aaaacccaca acggcaagct gtgtgacctg      180

gacggcgtga agcccctgat cctgagagac tgctccgtgg ccggctggct gctgggcaac      240

cccatgtgtg acgagttcat caacgtgccc gagtggagct acatcgtgga gaaggccagc      300

cccgccaacg acctgtgcta ccccggcgac ttcaacgact acgaggagct gaagcacctg      360

ctgtcccgga tcaaccactt cgagaagatc cagatcatcc ccaagagcag ctggagcaac      420

cacgaggcca gcagcggcgt gtccagcgcc tgcccctacc agggcaagag cagcttcttc      480

cggaacgtgg tctggctgat caagaagaac tctgcctatc ccaccatcaa gcggagctac      540

aacaacacca accaggagga tctgctggtc ctgtggggca tccaccaccc caacgacgcc      600

gccgagcaga ccaagctgta ccagaacccc accacctaca tcagcgtggg caccagcacc      660

ctgaaccagc ggctggtgcc caagatcgcc acccggtcca aagtgaacgg ccagagcggc      720

cggatggaat tcttctggac catcctgaag cccaacgatg ccatcaactt cgagagcaac      780

ggcaacttca tcgcccccga gtacgcctac aagatcgtga agaagggcga cagcgccatc      840

atgaagagcg agctggaata cggcaactgc aacaccaagt gccagacccc catgggcgcc      900

atcaacagca gcatgccctt ccacaacatc caccccctga ccatcggcga gtgccccaag      960

tacgtgaaga gcaacaggct ggtgctggcc accggcctgc ggaacagccc ccagcgggag     1020

cggcggagga agaagcgggg cctgttcggc gccatcgccg gcttcatcga gggcggctgg     1080

cagggcatgg tggacgggtg gtacggctac caccacagca atgagcaggg cagcggctac     1140

gccgccgaca aagagagcac ccagaaggcc atcgacggcg tcaccaacaa ggtgaacagc     1200

atcatcgaca agatgaacac ccagttcgag gccgtgggcc gggagttcaa caacctggaa     1260

cggcggatcg agaacctgaa caagaaaatg gaagatggct tcctggacgt gtggacctac     1320

aacgccgaac tcctggtgct gatggaaaac gagcggaccc tggacttcca cgacagcaac     1380

gtgaagaacc tgtacgacaa agtgcggctg cagctgcggg acaacgccaa agagctgggc     1440

aacggctgct tcgagttcta ccacaagtgc gacaacgagt gcatggaaag cgtgcggaac     1500

ggcacctacg actaccccca gtacagcgag gaagcccggc tgaagcggga ggaaatcagc     1560

ggcgtgaaac tggaaagcat cggcacctac cagatcctga gcatctacag caccgtggcc     1620

agcagcctcg ctctggccat tatggtggcc ggcctgagcc tgtggatgtg cagcaacggc     1680

agcctgcagt gccggatcgg atccagatct gctagcgtcg actctagatt aattaa         1736


<210>  3
<211>  567
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  3

Met Glu Lys Ile Val Leu Leu Leu Ala Ile Val Ser Leu Val Lys Ser 
1               5                   10                  15      


Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val 
            20                  25                  30          


Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile 
        35                  40                  45              


Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys 
    50                  55                  60                  


Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn 
65                  70                  75                  80  


Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val 
                85                  90                  95      


Glu Lys Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn 
            100                 105                 110         


Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu 
        115                 120                 125             


Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser 
    130                 135                 140                 


Ser Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly Lys Ser Ser Phe Phe 
145                 150                 155                 160 


Arg Asn Val Val Trp Leu Ile Lys Lys Asn Ser Ala Tyr Pro Thr Ile 
                165                 170                 175     


Lys Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp 
            180                 185                 190         


Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Arg Leu Tyr Gln 
        195                 200                 205             


Asn Pro Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg 
    210                 215                 220                 


Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly 
225                 230                 235                 240 


Arg Met Glu Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn 
                245                 250                 255     


Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile 
            260                 265                 270         


Val Lys Lys Gly Asp Ser Ala Ile Met Lys Ser Glu Leu Glu Tyr Gly 
        275                 280                 285             


Asn Cys Asn Thr Lys Cys Gln Thr Pro Met Gly Ala Ile Asn Ser Ser 
    290                 295                 300                 


Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys 
305                 310                 315                 320 


Tyr Val Lys Ser Asn Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Ser 
                325                 330                 335     


Pro Gln Arg Glu Arg Arg Arg Lys Arg Gly Leu Phe Gly Ala Ile Ala 
            340                 345                 350         


Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly 
        355                 360                 365             


Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu 
    370                 375                 380                 


Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser Ile 
385                 390                 395                 400 


Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe Asn 
                405                 410                 415     


Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp Gly 
            420                 425                 430         


Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu 
        435                 440                 445             


Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr 
    450                 455                 460                 


Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly Asn 
465                 470                 475                 480 


Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu Ser 
                485                 490                 495     


Val Arg Asn Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala Arg 
            500                 505                 510         


Leu Lys Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly Thr 
        515                 520                 525             


Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala Leu 
    530                 535                 540                 


Ala Ile Met Val Ala Gly Leu Ser Leu Trp Met Cys Ser Asn Gly Ser 
545                 550                 555                 560 


Leu Gln Cys Arg Ile Cys Ile 
                565         


<210>  4
<211>  1739
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Syntheric polynucleotide

<400>  4
atggagaaga tcgtgctgct gctggccatc gtgtccctgg tgaagagcga ccagatttgc       60

atcggctacc acgccaacaa tagcaccgag caagtggaca ccatcatgga gaaaaacgtg      120

accgtgaccc acgctcagga catcctcgaa aaaacccaca acggcaagct gtgcgatctg      180

gacggcgtga agcccctgat cctgagagac tgcagcgtgg ccggctggct gctgggcaat      240

cccatgtgcg acgagttcat caacgtgccc gagtggagct acatcgtgga gaaggccaac      300

cccgccaacg acctgtgcta ccccggcaac ttcaacgact acgaggagct gaagcacctg      360

ctgagccgga tcaaccactt cgagaagatc cagatcatcc ccaagagcag ctggagcgac      420

catgaggcaa gcagcggcgt gtccagcgcc tgcccctacc agggcaagtc cagcttcttc      480

cgcaacgttg tgtggctgat caagaagaac agcgcctacc ccaccatcaa gcggagctac      540

aacaacacca accaggagga cctgctggtc ctgtggggca tccaccaccc caacgacgcc      600

gccgagcaga cccggctgta ccagaacccc accacctaca tctctgtggg caccagcacc      660

ctgaaccagc ggctggtgcc caagatcgcc acccggagca aggtgaacgg ccagagcggc      720

cggatggagt tcttctggac catcctgaag cccaacgatg ccatcaactt cgagagcaac      780

ggcaacttca tcgcccccga gtacgcctac aagatcgtga agaagggcga cagcgccatc      840

atgaagtccg agctggagta cggcaactgt aacaccaagt gccagacccc catgggcgcc      900

atcaacagca gcatgccctt ccacaacatc caccccctga ccatcggcga gtgccccaag      960

tacgtgaaga gcaacaggct ggtgctggcc accggcctga gaaacagccc ccagagagag     1020

cggagaagaa agagaggcct gttcggcgcc attgccggct tcatcgaggg cggctggcag     1080

ggcatggtgg acgggtggta cggctaccac cactccaacg agcagggcag cggctacgcc     1140

gccgacaaag agagcaccca gaaagctatc gacggcgtga ccaacaaagt gaacagcatc     1200

atcgacaaga tgaataccca gttcgaggcc gtgggcagag agttcaacaa cctggaaaga     1260

agaatcgaga acctgaacaa gaaaatggaa gatggctttc tggatgtgtg gacctacaac     1320

gccgagctgc tggtgctgat ggaaaacgag cggaccctgg acttccacga cagcaacgtg     1380

aagaatctgt acgacaaagt gcggctgcag ctgagagaca acgccaaaga gctgggcaac     1440

ggctgcttcg agttctacca caagtgcgac aatgagtgca tggaaagcgt gcggaacggc     1500

acctacgact acccccagta cagcgaggaa gcccggctga agagagaaga gatttccggc     1560

gtgaagctgg aaagcatcgg cacctaccag atcctgagca tctacagcac cgtggccagc     1620

agcctggccc tggccatcat ggtggccggc ctgagcctgt ggatgtgcag caacggcagc     1680

ctgcagtgcc ggatctgcat cggatccaga tctgctagcg tcgactctag attaattaa      1739


