                         SEQUENCE LISTING

<110>  INSERM
 
<120>  METHOD OF PRODUCING HIGH-AFFINITY ANTIBODIES

<130>  BET13P0222

<150>  EP12305172.4
<151>  2012-02-15

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  2109
<212>  DNA
<213>  Mus musculus

<400>  1
gcagtcagtg ctcttaacca ctgagccatc tctccagctc gcccactggc ttccttgctt       60

gctttttctt aagttttatt tatttattta tttatttatt tatttattta gttatttagt      120

tatttattta gttatgtata ttggtatttt ttcgaagagg acatcagatt tcatcttaga      180

cggttgtgag ccaccatgtg aatgcagaga attgaaccca ggtcctctga aagaacagcc      240

agtgctctta aatactgaac catctctcca gcccctgctg ctccctgtcc ctctcccttt      300

taaagaaatg gtgtcagtca gaacaggcaa gatggttcca tggataaatg tccttgctgc      360

aaagcctgac tacttgagtt caagcctcag gatctacatg gtggacagag aggaccaagt      420

cttgtaaatt gtcttctgac atctacacat aagctctggc cctcgtgcct catatacccc      480

tccactgcca agcacagcaa tatatatata attttttaaa atgtaaagaa atcacaacat      540

ctctgccaat atccatcaag tcggcccttt gggaggctgt gtacgtgtgt ctcagtatgt      600

cattccctgg acaattggcc aaagtagggc aaaggtccgg gcctcatcct gtgagacaag      660

ttagagggac ttgtccaccc accacctggg ttcccttaac cctgtaatgt cacggctggt      720

gctggttact cccggtgccc tgaaattttt ttcccaggaa ttcattaatt cactagtgag      780

ggaaattgtg tctctgatag tgatgtgata atgcagagga aattaattag aggaagaagg      840

aggatggggg ctcattaaca tttcagatat gatatccagg gaaggctaaa ctgccaggga      900

gtaagccaag tcctgaacta tgagactttg cacagagaga tttcacagca acaaaatagg      960

ggcaggggca tgtgctgtgt gcatgcaacg ggatccagtc tctagctcaa gactggtctg     1020

gtctatatag aaagttccag accagccaga ggagctacat aatgacaccc tatctaaaaa     1080

aaggaaggga aggaaggaag gaaggaagga aggaagaaag gaaggaagga gggagggagg     1140

gagggaggga gggagggagg gaggaaagaa ggaaggaagg aaggaaggaa ggaaggaagg     1200

aaggaaggaa ggaaggaagg aaggaagagt ataagaaagg aaggaaggaa gaaagcaaag     1260

gatgttcttc cagatgatca gggttcagat cccagcaacc acacatggtg gctttcaacc     1320

gcctgctggt cctctgcaat agaaataagt gctcttaatc actgggccag ctctccaggc     1380

ctccagtaag gtatttttaa tgaggaaaaa gagttctttt ttaaaaaaaa aatacttttt     1440

gacacacaca cacacacaaa attaaaataa atcacttttt ggtgcaagca actagtcttt     1500

ctagctatct tataatgtca ttttaaaaaa agaaaaatat attagagaat taggaggcta     1560

aagttcactc tctggatgct gtggtggtca acccccatct ctactgaggc ataaaactga     1620

gtgtaacaaa cggaaggaac agatactgta agttcaagaa gcacaagatg catttaaggc     1680

cactttaagt cactatgact gctatcattc ttgttatcac aattttaaaa ttaggaagca     1740

tgcacagacc ttaggtgtga tacctgggac ccccccacac acacacacac acacactcac     1800

agagctcatt atcatgatac caatgtgaaa agtgtccagt gctattgtct cctgatcttt     1860

gttacctgtg gtacctgggc tggcttttta gaggaacagc ctcgaaggaa gttggacatt     1920

aagcatgagc agaactgccc cccgcccccc aatcatttaa tccgtgtggc tctgcccacc     1980

acagccccgc ccatctttac tggacccaac ccaggaggca gatgttggat acctggtggt     2040

agtgatgctg tcgtggggga ggagcccaca agagcaagct cagatttgaa tgccaggggc     2100

cagtgctct                                                             2109


<210>  2
<211>  347
<212>  PRT
<213>  Mus musculus

<400>  2

Met Ser Asn Leu Leu Thr Val His Gln Asn Leu Pro Ala Leu Pro Val 
1               5                   10                  15      


Asp Ala Thr Ser Asp Glu Val Arg Lys Asn Leu Met Asp Met Phe Arg 
            20                  25                  30          


Asp Arg Gln Ala Phe Ser Glu His Thr Trp Lys Met Leu Leu Ser Val 
        35                  40                  45              


Cys Arg Ser Trp Ala Ala Trp Cys Lys Leu Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  


Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu Tyr Leu Gln Ala 
65                  70                  75                  80  


Arg Gly Leu Ala Val Lys Thr Ile Gln Gln His Leu Gly Gln Leu Asn 
                85                  90                  95      


Met Leu His Arg Arg Ser Gly Leu Pro Arg Pro Ser Asp Ser Asn Ala 
            100                 105                 110         


Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly 
        115                 120                 125             


Glu Arg Ala Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 


Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 


Leu Ala Phe Leu Gly Ile Ala Tyr Asn Thr Leu Leu Arg Ile Ala Glu 
                165                 170                 175     


Ile Ala Arg Ile Arg Val Lys Asp Ile Ser Arg Thr Asp Gly Gly Arg 
            180                 185                 190         


Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Thr Ala Gly 
        195                 200                 205             


Val Glu Lys Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 


Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 


Arg Val Arg Lys Asn Gly Val Ala Ala Pro Ser Ala Thr Ser Gln Leu 
                245                 250                 255     


Ser Thr Arg Ala Leu Glu Gly Ile Phe Glu Ala Thr His Arg Leu Ile 
            260                 265                 270         


Tyr Gly Ala Lys Asp Asp Ser Gly Gln Arg Tyr Leu Ala Trp Ser Gly 
        275                 280                 285             


His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 


Ser Ile Pro Glu Ile Met Gln Ala Gly Gly Trp Thr Asn Val Asn Ile 
305                 310                 315                 320 


Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     


Arg Leu Leu Glu Asp Gly Asp Leu Glu Pro Ser 
            340                 345         


<210>  3
<211>  660
<212>  PRT
<213>  Mus musculus

<400>  3

Met Ser Asn Leu Leu Thr Val His Gln Asn Leu Pro Ala Leu Pro Val 
1               5                   10                  15      


Asp Ala Thr Ser Asp Glu Val Arg Lys Asn Leu Met Asp Met Phe Arg 
            20                  25                  30          


Asp Arg Gln Ala Phe Ser Glu His Thr Trp Lys Met Leu Leu Ser Val 
        35                  40                  45              


Cys Arg Ser Trp Ala Ala Trp Cys Lys Leu Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  


Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu Tyr Leu Gln Ala 
65                  70                  75                  80  


Arg Gly Leu Ala Val Lys Thr Ile Gln Gln His Leu Gly Gln Leu Asn 
                85                  90                  95      


Met Leu His Arg Arg Ser Gly Leu Pro Arg Pro Ser Asp Ser Asn Ala 
            100                 105                 110         


Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly 
        115                 120                 125             


Glu Arg Ala Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 


Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 


Leu Ala Phe Leu Gly Ile Ala Tyr Asn Thr Leu Leu Arg Ile Ala Glu 
                165                 170                 175     


Ile Ala Arg Ile Arg Val Lys Asp Ile Ser Arg Thr Asp Gly Gly Arg 
            180                 185                 190         


Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Thr Ala Gly 
        195                 200                 205             


Val Glu Lys Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 


Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 


Arg Val Arg Lys Asn Gly Val Ala Ala Pro Ser Ala Thr Ser Gln Leu 
                245                 250                 255     


Ser Thr Arg Ala Leu Glu Gly Ile Phe Glu Ala Thr His Arg Leu Ile 
            260                 265                 270         


Tyr Gly Ala Lys Asp Asp Ser Gly Gln Arg Tyr Leu Ala Trp Ser Gly 
        275                 280                 285             


His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 


Ser Ile Pro Glu Ile Met Gln Ala Gly Gly Trp Thr Asn Val Asn Ile 
305                 310                 315                 320 


Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     


Arg Leu Leu Glu Asp Gly Asp Leu Glu Pro Ser Ala Gly Asp Met Arg 
            340                 345                 350         


Ala Ala Asn Leu Trp Pro Ser Pro Leu Met Ile Lys Arg Ser Lys Lys 
        355                 360                 365             


Asn Ser Leu Ala Leu Ser Leu Thr Ala Asp Gln Met Val Ser Ala Leu 
    370                 375                 380                 


Leu Asp Ala Glu Pro Pro Ile Leu Tyr Ser Glu Tyr Asp Pro Thr Arg 
385                 390                 395                 400 


Pro Phe Ser Glu Ala Ser Met Met Gly Leu Leu Thr Asn Leu Ala Asp 
                405                 410                 415     


Arg Glu Leu Val His Met Ile Asn Trp Ala Lys Arg Val Pro Gly Phe 
            420                 425                 430         


Val Asp Leu Thr Leu His Asp Gln Val His Leu Leu Glu Cys Ala Trp 
        435                 440                 445             


Leu Glu Ile Leu Met Ile Gly Leu Val Trp Arg Ser Met Glu His Pro 
    450                 455                 460                 


Val Lys Leu Leu Phe Ala Pro Asn Leu Leu Leu Asp Arg Asn Gln Gly 
465                 470                 475                 480 


Lys Cys Val Glu Gly Met Val Glu Ile Phe Asp Met Leu Leu Ala Thr 
                485                 490                 495     


Ser Ser Arg Phe Arg Met Met Asn Leu Gln Gly Glu Glu Phe Val Cys 
            500                 505                 510         


Leu Lys Ser Ile Ile Leu Leu Asn Ser Gly Val Tyr Thr Phe Leu Ser 
        515                 520                 525             


Ser Thr Leu Lys Ser Leu Glu Glu Lys Asp His Ile His Arg Val Leu 
    530                 535                 540                 


Asp Lys Ile Thr Asp Thr Leu Ile His Leu Met Ala Lys Ala Gly Leu 
545                 550                 555                 560 


Thr Leu Gln Gln Gln His Gln Arg Leu Ala Gln Leu Leu Leu Ile Leu 
                565                 570                 575     


Ser His Ile Arg His Met Ser Asn Lys Gly Met Glu His Leu Tyr Ser 
            580                 585                 590         


Met Lys Cys Lys Asn Val Val Pro Leu Tyr Asp Leu Leu Leu Glu Ala 
        595                 600                 605             


Ala Asp Ala His Arg Leu His Ala Pro Thr Ser Arg Gly Gly Ala Ser 
    610                 615                 620                 


Val Glu Glu Thr Asp Gln Ser His Leu Ala Thr Ala Gly Ser Thr Ser 
625                 630                 635                 640 


Ser His Ser Leu Gln Lys Tyr Tyr Ile Thr Gly Glu Ala Glu Gly Phe 
                645                 650                 655     


Pro Ala Thr Ala 
            660 


<210>  4
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-ext-primer

<400>  4
tctccatgaa agtcaggctg gcttcc                                            26


<210>  5
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'-Cre-primer

<400>  5
ctcatcactc gttgcatcga ccgg                                              24


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Neo-S primer

<400>  6
catagcgttg gctacccgtg                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Neo-As primer

<400>  7
cacgggtagc caacgctatg                                                   20


<210>  8
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'-ext-primer

<400>  8
ccattgcacc agagtatgaa atagcttccg                                        30


<210>  9
<211>  914
<212>  DNA
<213>  Mus musculus

<400>  9
ttttaaagca aaattttgga ctgtgaagca aggcactggg tgagtaaatc aaatcaaatc       60

aaattatatg ctctaaattt tgtaggtgat tttttttctc atgaatttta cccgtgtgtg      120

tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg aagtttgcca tcttagactt      180

gatgaacaaa ggatattgaa ttctctccat acaaatctca ggtttcaccc ctctggccct      240

tcactaactg acttcatacc gttgtatgta tggtattatt tgtccccgta ctctttctca      300

gttcaggcta acctggttgc ctacagtgcc aggattataa gagtgctaag tactcaaact      360

attgaacgaa aaaactcaca atctcagagc atgggatgaa tagtgaaatg acattccgat      420

aaaaatagct acaccttttt ttttttaaga acttattatg gtgtaggcac atatttctaa      480

ttttaaaaac cacataggta ttgctgctcc tattttggaa aatgaaagct cagaaacagt      540

accagcacac tccaagtaac agagctggtg aaaggaaaag cagcctgggt aactctccaa      600

gagtggtttt ctccattgca acacactcag ggcctctcaa tgcttggcag agtgtttagt      660

atgcggaaaa gcactttaca agcagccctt cccttcccct ttcccccaaa gagctttcta      720

cactggcttt cattctcccc atttgacagc tatgcaaagc aaagtccaga aatgggctat      780

gaattgtcca ctatatacct gagttgcatg cgatagctta tcaggctaag tcatctgctt      840

ggtcctcccg gtaggcaggg actaggttct caaggagtaa taggagtttt aacgtctgtt      900

caggcaaaca caac                                                        914


