                               SEQUENCE LISTING

<110> DISCOVERX CORPORATION

<120> DETECTION OF INTRACELLULAR BINDING EVENTS BY MEASURING PROTEIN 
      ABUNDANCE

<130> 3817.41-1 PCT

<140>
<141>

<150> 61/595,525
<151> 2012-02-06

<160> 20    

<170> PatentIn version 3.5

<210> 1
<211> 52
<212> PRT
<213> Escherichia coli

<400> 1
Met Gly Val Ile Thr Asp Ser Leu Ala Val Val Leu Gln Arg Arg Asp 
1               5                   10                  15      


Trp Glu Asn Pro Gly Val Thr Gln Leu Asn Arg Leu Ala Ala His Pro 
            20                  25                  30          


Pro Phe Ala Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg Pro 
        35                  40                  45              


Ser Gln Gln Leu 
    50          


<210> 2
<211> 42
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 2
Asn Ser Leu Ala Val Val Leu Gln Arg Arg Asp Trp Glu Asn Pro Gly 
1               5                   10                  15      


Val Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp 
            20                  25                  30          


Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg 
        35                  40          


<210> 3
<211> 52
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 3
Met Gly Val Ile Thr Asp Ser Leu Ala Val Val Leu Gln Arg Arg Asp 
1               5                   10                  15      


Trp Glu Asn Pro Gly Val Thr Gln Leu Asn Arg Leu Ala Ala His Pro 
            20                  25                  30          


Pro Phe Ala Ser Tyr Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg Pro 
        35                  40                  45              


Ser Gln Gln Leu 
    50          


<210> 4
<211> 42
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 4
Asn Ser Leu Ala Val Val Leu Gln Arg Arg Asp Trp Glu Asn Pro Gly 
1               5                   10                  15      


Val Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp 
            20                  25                  30          


Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg 
        35                  40          


<210> 5
<211> 991
<212> PRT
<213> Escherichia coli

<400> 5
Met Gly Val Ile Thr Asp Ser Leu Ala Val Val Ala Arg Thr Asp Arg 
1               5                   10                  15      


Pro Ser Gln Gln Leu Arg Ser Leu Asn Gly Glu Trp Arg Phe Ala Trp 
            20                  25                  30          


Phe Pro Ala Pro Glu Ala Val Pro Glu Ser Trp Leu Glu Cys Asp Leu 
        35                  40                  45              


Pro Glu Ala Asp Thr Val Val Val Pro Ser Asn Trp Gln Met His Gly 
    50                  55                  60                  


Tyr Asp Ala Pro Ile Tyr Thr Asn Val Thr Tyr Pro Ile Thr Val Asn 
65                  70                  75                  80  


Pro Pro Phe Val Pro Thr Glu Asn Pro Thr Gly Cys Tyr Ser Leu Thr 
                85                  90                  95      


Phe Asn Val Asp Glu Ser Trp Leu Gln Glu Gly Gln Thr Arg Ile Ile 
            100                 105                 110         


Phe Asp Gly Val Asn Ser Ala Phe His Leu Trp Cys Asn Gly Arg Trp 
        115                 120                 125             


Val Gly Tyr Gly Gln Asp Ser Arg Leu Pro Ser Glu Phe Asp Leu Ser 
    130                 135                 140                 


Ala Phe Leu Arg Ala Gly Glu Asn Arg Leu Ala Val Met Val Leu Arg 
145                 150                 155                 160 


Trp Ser Asp Gly Ser Tyr Leu Glu Asp Gln Asp Met Trp Arg Met Ser 
                165                 170                 175     


Gly Ile Phe Arg Asp Val Ser Leu Leu His Lys Pro Thr Thr Gln Ile 
            180                 185                 190         


Ser Asp Phe His Val Ala Thr Arg Phe Asn Asp Asp Phe Ser Arg Ala 
        195                 200                 205             


Val Leu Glu Ala Glu Val Gln Met Cys Gly Glu Leu Arg Asp Tyr Leu 
    210                 215                 220                 


Arg Val Thr Val Ser Leu Trp Gln Gly Glu Thr Gln Val Ala Ser Gly 
225                 230                 235                 240 


Thr Ala Pro Phe Gly Gly Glu Ile Ile Asp Glu Arg Gly Gly Tyr Ala 
                245                 250                 255     


Asp Arg Val Thr Leu Arg Leu Asn Val Glu Asn Pro Lys Leu Trp Ser 
            260                 265                 270         


Ala Glu Ile Pro Asn Leu Tyr Arg Ala Val Val Glu Leu His Thr Ala 
        275                 280                 285             


Asp Gly Thr Leu Ile Glu Ala Glu Cys Asp Val Gly Phe Arg Glu Val 
    290                 295                 300                 


Arg Ile Glu Asn Gly Leu Leu Leu Leu Asn Gly Lys Pro Leu Leu Ile 
305                 310                 315                 320 


Arg Gly Val Asn Arg His Glu His His Pro Leu His Gly Gln Val Met 
                325                 330                 335     


Asp Glu Gln Thr Met Val Gln Asp Ile Leu Leu Met Lys Gln Asn Asn 
            340                 345                 350         


Phe Asn Ala Val Arg Cys Ser His Tyr Pro Asn His Pro Leu Trp Tyr 
        355                 360                 365             


Thr Leu Cys Asp Arg Tyr Gly Leu Tyr Val Val Asp Glu Ala Asn Ile 
    370                 375                 380                 


Glu Thr His Gly Met Val Pro Met Asn Arg Leu Thr Asp Asp Pro Arg 
385                 390                 395                 400 


Trp Leu Pro Ala Met Ser Glu Arg Val Thr Arg Met Val Gln Arg Asp 
                405                 410                 415     


Arg Asn His Pro Ser Val Ile Ile Trp Ser Leu Gly Asn Glu Ser Gly 
            420                 425                 430         


His Gly Ala Asn His Asp Ala Leu Tyr Arg Trp Ile Lys Ser Val Asp 
        435                 440                 445             


Pro Ser Arg Pro Val Gln Tyr Glu Gly Gly Gly Ala Asp Thr Thr Ala 
    450                 455                 460                 


Thr Asp Ile Ile Cys Pro Met Tyr Ala Arg Val Asp Glu Asp Gln Pro 
465                 470                 475                 480 


Phe Pro Ala Val Pro Lys Trp Ser Ile Lys Lys Trp Leu Ser Leu Pro 
                485                 490                 495     


Gly Glu Thr Arg Pro Leu Ile Leu Cys Glu Tyr Ala His Ala Met Gly 
            500                 505                 510         


Asn Ser Leu Gly Gly Phe Ala Lys Tyr Trp Gln Ala Phe Arg Gln Tyr 
        515                 520                 525             


Pro Arg Leu Gln Gly Gly Phe Val Trp Asp Trp Val Asp Gln Ser Leu 
    530                 535                 540                 


Ile Lys Tyr Asp Glu Asn Asn Pro Trp Ser Ala Tyr Gly Gly Asp Phe 
545                 550                 555                 560 


Gly Asp Thr Pro Asn Asp Arg Gln Phe Cys Met Asn Gly Leu Val Phe 
                565                 570                 575     


Ala Asp Arg Thr Pro His Pro Ala Leu Thr Glu Ala Lys His Gln Gln 
            580                 585                 590         


Gln Phe Phe Gln Phe Arg Leu Ser Gly Gln Thr Ile Glu Val Thr Ser 
        595                 600                 605             


Glu Tyr Leu Phe Arg His Ser Asp Asn Glu Leu Leu His Trp Met Val 
    610                 615                 620                 


Ala Leu Asp Gly Lys Pro Leu Ala Ser Gly Glu Val Pro Leu Asp Val 
625                 630                 635                 640 


Ala Pro Gln Gly Lys Gln Leu Ile Glu Leu Pro Glu Leu Pro Gln Pro 
                645                 650                 655     


Glu Ser Ala Gly Gln Leu Trp Leu Thr Val Arg Val Val Gln Pro Asn 
            660                 665                 670         


Ala Thr Ala Trp Ser Glu Ala Gly His Ile Ser Ala Trp Gln Gln Trp 
        675                 680                 685             


Arg Leu Ala Glu Asn Leu Ser Val Thr Leu Pro Ala Ala Ser His Ala 
    690                 695                 700                 


Ile Pro His Leu Thr Thr Ser Glu Met Asp Phe Cys Ile Glu Leu Gly 
705                 710                 715                 720 


Asn Lys Arg Trp Gln Phe Asn Arg Gln Ser Gly Phe Leu Ser Gln Met 
                725                 730                 735     


Trp Ile Gly Asp Lys Lys Gln Leu Leu Thr Pro Leu Arg Asp Gln Phe 
            740                 745                 750         


Thr Arg Ala Pro Leu Asp Asn Asp Ile Gly Val Ser Glu Ala Thr Arg 
        755                 760                 765             


Ile Asp Pro Asn Ala Trp Val Glu Arg Trp Lys Ala Ala Gly His Tyr 
    770                 775                 780                 


Gln Ala Glu Ala Ala Leu Leu Gln Cys Thr Ala Asp Thr Leu Ala Asp 
785                 790                 795                 800 


Ala Val Leu Ile Thr Thr Ala His Ala Trp Gln His Gln Gly Lys Thr 
                805                 810                 815     


Leu Phe Ile Ser Arg Lys Thr Tyr Arg Ile Asp Gly Ser Gly Gln Met 
            820                 825                 830         


Ala Ile Thr Val Asp Val Glu Val Ala Ser Asp Thr Pro His Pro Ala 
        835                 840                 845             


Arg Ile Gly Leu Asn Cys Gln Leu Ala Gln Val Ala Glu Arg Val Asn 
    850                 855                 860                 


Trp Leu Gly Leu Gly Pro Gln Glu Asn Tyr Pro Asp Arg Leu Thr Ala 
865                 870                 875                 880 


Ala Cys Phe Asp Arg Trp Asp Leu Pro Leu Ser Asp Met Tyr Thr Pro 
                885                 890                 895     


Tyr Val Phe Pro Ser Glu Asn Gly Leu Arg Cys Gly Thr Arg Glu Leu 
            900                 905                 910         


Asn Tyr Gly Pro His Gln Trp Arg Gly Asp Phe Gln Phe Asn Ile Ser 
        915                 920                 925             


Arg Tyr Ser Gln Gln Gln Leu Met Glu Thr Ser His Arg His Leu Leu 
    930                 935                 940                 


His Ala Glu Glu Gly Thr Trp Leu Asn Ile Asp Gly Phe His Met Gly 
945                 950                 955                 960 


Ile Gly Gly Asp Asp Ser Trp Ser Pro Ser Val Ser Ala Glu Phe Gln 
                965                 970                 975     


Leu Ser Ala Gly Arg Tyr His Tyr Gln Leu Val Trp Cys Gln Lys 
            980                 985                 990     


<210> 6
<211> 4187
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 6
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg      660

gatccaccgg ccggtcgcca ccatgagctc caattcactg gccgtcgttt tacaacgtcg      720

tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc      780

cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct      840

gaatggcgaa taggcggccg cgactctaga tcataatcag ccataccaca tttgtagagg      900

ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg      960

caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca     1020

tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac     1080

tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta     1140

aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat     1200

aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca     1260

ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc     1320

ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta     1380

aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg     1440

gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg     1500

gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca     1560

ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat     1620

tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa     1680

aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa     1740

agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa     1800

ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca     1860

attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca     1920

gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg     1980

ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct     2040

tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga     2100

ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa     2160

cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt     2220

ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg     2280

ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa     2340

gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac     2400

cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt     2460

gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact     2520

cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg     2580

ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg     2640

acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc     2700

atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt     2760

gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc     2820

gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg     2880

ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg     2940

attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct     3000

ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc     3060

taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa     3120

gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg     3180

gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt     3240

ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg     3300

gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac     3360

ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa     3420

tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat     3480

cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc     3540

taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg     3600

gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc     3660

acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg     3720

ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg     3780

ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa     3840

cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg     3900

aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga     3960

gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct     4020

gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca     4080

gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc     4140

ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat                   4187


<210> 7
<211> 302
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 7
Met Leu Arg Leu Val Arg Lys Leu Gly Ser Gly Gln Phe Gly Glu Val 
1               5                   10                  15      


Trp Met Gly Tyr Tyr Lys Asn Asn Met Lys Val Ala Ile Lys Thr Leu 
            20                  25                  30          


Lys Glu Gly Thr Met Ser Pro Glu Ala Phe Leu Gly Glu Ala Asn Val 
        35                  40                  45              


Met Lys Ala Leu Gln His Glu Arg Leu Val Arg Leu Tyr Ala Val Val 
    50                  55                  60                  


Thr Lys Glu Pro Ile Tyr Ile Val Thr Glu Tyr Met Ala Arg Gly Cys 
65                  70                  75                  80  


Leu Leu Asp Phe Leu Lys Thr Asp Glu Gly Ser Arg Leu Ser Leu Pro 
                85                  90                  95      


Arg Leu Ile Asp Met Ser Ala Gln Ile Ala Glu Gly Met Ala Tyr Ile 
            100                 105                 110         


Glu Arg Met Asn Ser Ile His Arg Asp Leu Arg Ala Ala Asn Ile Leu 
        115                 120                 125             


Val Ser Glu Ala Leu Cys Cys Lys Ile Ala Asp Phe Gly Leu Ala Arg 
    130                 135                 140                 


Ile Ile Asp Ser Glu Tyr Thr Ala Gln Glu Gly Ala Lys Phe Pro Ile 
145                 150                 155                 160 


Lys Trp Thr Ala Pro Glu Ala Ile His Phe Gly Val Phe Thr Ile Lys 
                165                 170                 175     


Ala Asp Val Trp Ser Phe Gly Val Leu Leu Met Glu Val Val Thr Tyr 
            180                 185                 190         


Gly Arg Val Pro Tyr Pro Gly Met Ser Asn Pro Glu Val Ile Arg Asn 
        195                 200                 205             


Leu Glu Arg Gly Tyr Arg Met Pro Arg Pro Asp Thr Cys Pro Pro Glu 
    210                 215                 220                 


Leu Tyr Arg Gly Val Ile Ala Glu Cys Trp Arg Ser Arg Pro Glu Glu 
225                 230                 235                 240 


Arg Pro Thr Phe Glu Phe Leu Gln Ser Val Leu Ser Ser Phe Glu Leu 
                245                 250                 255     


Leu Glu Ser Ser Asn Ser Leu Ala Val Val Leu Gln Arg Arg Asp Trp 
            260                 265                 270         


Glu Asn Pro Gly Val Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro 
        275                 280                 285             


Phe Ala Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg 
    290                 295                 300         


<210> 8
<211> 20
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: Truncated BIM peptide

<400> 8
Met Arg Pro Glu Ile Trp Ile Ala Gln Glu Leu Arg Arg Ile Gly Asp 
1               5                   10                  15      


Glu Phe Asn Ala 
            20  


<210> 9
<211> 16
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: Truncated BAK peptide

<400> 9
Gly Gln Val Gly Arg Gln Leu Ala Ile Ile Gly Asp Asp Ile Asn Arg 
1               5                   10                  15      


<210> 10
<211> 21
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: Truncated BAX peptide

<400> 10
Gln Asp Ala Ser Thr Lys Lys Leu Ser Glu Cys Leu Lys Arg Ile Gly 
1               5                   10                  15      


Asp Glu Leu Asp Ser 
            20      


<210> 11
<211> 71
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 11
Met Arg Pro Glu Ile Trp Ile Ala Gln Glu Leu Arg Arg Ile Gly Asp 
1               5                   10                  15      


Glu Phe Asn Ala Ser Ser Phe Glu Leu Leu Glu Ser Ser Asn Ser Leu 
            20                  25                  30          


Ala Val Val Leu Gln Arg Arg Asp Trp Glu Asn Pro Gly Val Thr Gln 
        35                  40                  45              


Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp Arg Asn Ser 
    50                  55                  60                  


Glu Glu Ala Arg Thr Asp Arg 
65                  70      


<210> 12
<211> 366
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 12
Met Gln Lys Val Gly Glu Leu Lys Asp Asp Asp Phe Glu Lys Ile Ser 
1               5                   10                  15      


Glu Leu Gly Ala Gly Asn Gly Gly Val Val Phe Lys Val Ser His Lys 
            20                  25                  30          


Pro Ser Gly Leu Val Met Ala Arg Lys Leu Ile His Leu Glu Ile Lys 
        35                  40                  45              


Pro Ala Ile Arg Asn Gln Ile Ile Arg Glu Leu Gln Val Leu His Glu 
    50                  55                  60                  


Cys Asn Ser Pro Tyr Ile Val Gly Phe Tyr Gly Ala Phe Tyr Ser Asp 
65                  70                  75                  80  


Gly Glu Ile Ser Ile Cys Met Glu His Met Asp Gly Gly Ser Leu Asp 
                85                  90                  95      


Gln Val Leu Lys Lys Ala Gly Arg Ile Pro Glu Gln Ile Leu Gly Lys 
            100                 105                 110         


Val Ser Ile Ala Val Ile Lys Gly Leu Thr Tyr Leu Arg Glu Lys His 
        115                 120                 125             


Lys Ile Met His Arg Asp Val Lys Pro Ser Asn Ile Leu Val Asn Ser 
    130                 135                 140                 


Arg Gly Glu Ile Lys Leu Cys Asp Phe Gly Val Ser Gly Gln Leu Ile 
145                 150                 155                 160 


Asp Ser Met Ala Asn Ser Phe Val Gly Thr Arg Ser Tyr Met Ser Pro 
                165                 170                 175     


Glu Arg Leu Gln Gly Thr His Tyr Ser Val Gln Ser Asp Ile Trp Ser 
            180                 185                 190         


Met Gly Leu Ser Leu Val Glu Met Ala Val Gly Arg Tyr Pro Ile Pro 
        195                 200                 205             


Pro Pro Asp Ala Lys Glu Leu Glu Leu Met Phe Gly Cys Gln Val Glu 
    210                 215                 220                 


Gly Asp Ala Ala Glu Thr Pro Pro Arg Pro Arg Thr Pro Gly Arg Pro 
225                 230                 235                 240 


Leu Ser Ser Tyr Gly Met Asp Ser Arg Pro Pro Met Ala Ile Phe Glu 
                245                 250                 255     


Leu Leu Asp Tyr Ile Val Asn Glu Pro Pro Pro Lys Leu Pro Ser Gly 
            260                 265                 270         


Val Phe Ser Leu Glu Phe Gln Asp Phe Val Asn Lys Cys Leu Ile Lys 
        275                 280                 285             


Asn Pro Ala Glu Arg Ala Asp Leu Lys Gln Leu Met Val His Ala Phe 
    290                 295                 300                 


Ile Lys Arg Ser Asp Ala Glu Glu Val Asp Phe Ser Ser Phe Glu Leu 
305                 310                 315                 320 


Leu Glu Ser Ser Asn Ser Leu Ala Val Val Leu Gln Arg Arg Asp Trp 
                325                 330                 335     


Glu Asn Pro Gly Val Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro 
            340                 345                 350         


Phe Ala Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg 
        355                 360                 365     


<210> 13
<211> 162
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 13
Met Lys Val Ser Glu Gln Leu Lys Cys Cys Ser Gly Ile Leu Lys Glu 
1               5                   10                  15      


Met Phe Ala Lys Lys His Ala Ala Tyr Ala Trp Pro Phe Tyr Lys Pro 
            20                  25                  30          


Val Asp Val Glu Ala Leu Gly Leu His Asp Tyr Cys Asp Ile Ile Lys 
        35                  40                  45              


His Pro Met Asp Met Ser Thr Ile Lys Ser Lys Leu Glu Ala Arg Glu 
    50                  55                  60                  


Tyr Arg Asp Ala Gln Glu Phe Gly Ala Asp Val Arg Leu Met Phe Ser 
65                  70                  75                  80  


Asn Cys Tyr Lys Tyr Asn Pro Pro Asp His Glu Val Val Ala Met Ala 
                85                  90                  95      


Arg Lys Leu Gln Asp Val Phe Glu Met Arg Phe Ala Lys Met Pro Ser 
            100                 105                 110         


Ser Phe Glu Leu Leu Glu Ser Ser Asn Ser Leu Ala Val Val Leu Gln 
        115                 120                 125             


Arg Arg Asp Trp Glu Asn Pro Gly Val Thr Gln Leu Asn Arg Leu Ala 
    130                 135                 140                 


Ala His Pro Pro Phe Ala Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr 
145                 150                 155                 160 


Asp Arg 
        


<210> 14
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 14
Met Arg Val Thr Asn Gln Leu Gln Tyr Leu His Lys Val Val Met Lys 
1               5                   10                  15      


Ala Leu Trp Lys His Gln Phe Ala Trp Pro Phe Arg Gln Pro Val Asp 
            20                  25                  30          


Ala Val Lys Leu Gly Leu Pro Asp Tyr His Lys Ile Ile Lys Gln Pro 
        35                  40                  45              


Met Asp Met Gly Thr Ile Lys Arg Arg Leu Glu Asn Asn Tyr Tyr Trp 
    50                  55                  60                  


Ala Ala Ser Glu Cys Met Gln Asp Phe Asn Thr Met Phe Thr Asn Cys 
65                  70                  75                  80  


Tyr Ile Tyr Asn Lys Pro Thr Asp Asp Ile Val Leu Met Ala Gln Thr 
                85                  90                  95      


Leu Glu Lys Ile Phe Leu Gln Lys Val Ala Ser Met Pro Gln Glu Glu 
            100                 105                 110         


Gln Glu Leu Val Ser Ser Phe Glu Leu Leu Glu Ser Ser Asn Ser Leu 
        115                 120                 125             


Ala Val Val Leu Gln Arg Arg Asp Trp Glu Asn Pro Gly Val Thr Gln 
    130                 135                 140                 


Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp Arg Asn Ser 
145                 150                 155                 160 


Glu Glu Ala Arg Thr Asp Arg 
                165         


<210> 15
<211> 155
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 15
Met Leu Thr Asn Gln Leu Gln Tyr Leu Gln Lys Val Val Leu Lys Asp 
1               5                   10                  15      


Leu Trp Lys His Ser Phe Ser Trp Pro Phe Gln Arg Pro Val Asp Ala 
            20                  25                  30          


Val Lys Leu Gln Leu Pro Asp Tyr Tyr Thr Ile Ile Lys Asn Pro Met 
        35                  40                  45              


Asp Leu Asn Thr Ile Lys Lys Arg Leu Glu Asn Lys Tyr Tyr Ala Lys 
    50                  55                  60                  


Ala Ser Glu Cys Ile Glu Asp Phe Asn Thr Met Phe Ser Asn Cys Tyr 
65                  70                  75                  80  


Leu Tyr Asn Lys Pro Gly Asp Asp Ile Val Leu Met Ala Gln Ala Leu 
                85                  90                  95      


Glu Lys Leu Phe Met Gln Lys Leu Ser Ser Phe Glu Leu Leu Glu Ser 
            100                 105                 110         


Ser Asn Ser Leu Ala Val Val Leu Gln Arg Arg Asp Trp Glu Asn Pro 
        115                 120                 125             


Gly Val Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser 
    130                 135                 140                 


Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg 
145                 150                 155 


<210> 16
<211> 458
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 16
Met Ser Thr Ala Thr Thr Val Ala Pro Ala Gly Ile Pro Ala Thr Pro 
1               5                   10                  15      


Gly Pro Val Asn Pro Pro Pro Pro Glu Val Ser Asn Pro Ser Lys Pro 
            20                  25                  30          


Gly Arg Lys Thr Asn Gln Leu Gln Tyr Met Gln Asn Val Val Val Lys 
        35                  40                  45              


Thr Leu Trp Lys His Gln Phe Ala Trp Pro Phe Tyr Gln Pro Val Asp 
    50                  55                  60                  


Ala Ile Lys Leu Asn Leu Pro Asp Tyr His Lys Ile Ile Lys Asn Pro 
65                  70                  75                  80  


Met Asp Met Gly Thr Ile Lys Lys Arg Leu Glu Asn Asn Tyr Tyr Trp 
                85                  90                  95      


Ser Ala Ser Glu Cys Met Gln Asp Phe Asn Thr Met Phe Thr Asn Cys 
            100                 105                 110         


Tyr Ile Tyr Asn Lys Pro Thr Asp Asp Ile Val Leu Met Ala Gln Ala 
        115                 120                 125             


Leu Glu Lys Ile Phe Leu Gln Lys Val Ala Gln Met Pro Gln Glu Glu 
    130                 135                 140                 


Val Glu Leu Leu Pro Pro Ala Pro Lys Gly Lys Gly Arg Lys Pro Ala 
145                 150                 155                 160 


Ala Gly Ala Gln Ser Ala Gly Thr Gln Gln Val Ala Ala Val Ser Ser 
                165                 170                 175     


Val Ser Pro Ala Thr Pro Phe Gln Ser Val Pro Pro Thr Val Ser Gln 
            180                 185                 190         


Thr Pro Val Ile Ala Ala Thr Pro Val Pro Thr Ile Thr Ala Asn Val 
        195                 200                 205             


Thr Ser Val Pro Val Pro Pro Ala Ala Ala Pro Pro Pro Pro Ala Thr 
    210                 215                 220                 


Pro Ile Val Pro Val Val Pro Pro Thr Pro Pro Val Val Lys Lys Lys 
225                 230                 235                 240 


Gly Val Lys Arg Lys Ala Asp Thr Thr Thr Pro Thr Thr Ser Ala Ile 
                245                 250                 255     


Thr Ala Ser Arg Ser Glu Ser Pro Pro Pro Leu Ser Asp Pro Lys Gln 
            260                 265                 270         


Ala Lys Val Val Ala Arg Arg Glu Ser Gly Gly Arg Pro Ile Lys Pro 
        275                 280                 285             


Pro Lys Lys Asp Leu Glu Asp Gly Glu Val Pro Gln His Ala Gly Lys 
    290                 295                 300                 


Lys Gly Lys Leu Ser Glu His Leu Arg Tyr Cys Asp Ser Ile Leu Arg 
305                 310                 315                 320 


Glu Met Leu Ser Lys Lys His Ala Ala Tyr Ala Trp Pro Phe Tyr Lys 
                325                 330                 335     


Pro Val Asp Ala Glu Ala Leu Glu Leu His Asp Tyr His Asp Ile Ile 
            340                 345                 350         


Lys His Pro Met Asp Leu Ser Thr Val Lys Arg Lys Met Asp Gly Arg 
        355                 360                 365             


Glu Tyr Pro Asp Ala Gln Gly Phe Ala Ala Asp Val Arg Leu Met Phe 
    370                 375                 380                 


Ser Asn Cys Tyr Lys Tyr Asn Pro Pro Asp His Glu Val Val Ala Met 
385                 390                 395                 400 


Ala Arg Lys Ser Asn Cys Cys Ile Ser Phe Glu Leu Leu Glu Ser Ser 
                405                 410                 415     


Asn Ser Leu Ala Val Val Leu Gln Arg Arg Asp Trp Glu Asn Pro Gly 
            420                 425                 430         


Val Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp 
        435                 440                 445             


Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg 
    450                 455             


<210> 17
<211> 328
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 17
Met Thr Glu Lys Ile Ile Cys Arg Asp Val Ala Arg Gly Tyr Glu Asn 
1               5                   10                  15      


Val Pro Ile Pro Cys Val Asn Gly Val Asp Gly Glu Pro Cys Pro Glu 
            20                  25                  30          


Asp Tyr Lys Tyr Ile Ser Glu Asn Cys Glu Thr Ser Thr Met Asn Ile 
        35                  40                  45              


Asp Arg Asn Ile Thr His Leu Gln His Cys Thr Cys Val Asp Asp Cys 
    50                  55                  60                  


Ser Ser Ser Asn Cys Leu Cys Gly Gln Leu Ser Ile Arg Cys Trp Tyr 
65                  70                  75                  80  


Asp Lys Asp Gly Arg Leu Leu Gln Glu Phe Asn Lys Ile Glu Pro Pro 
                85                  90                  95      


Leu Ile Phe Glu Cys Asn Gln Ala Cys Ser Cys Trp Arg Asn Cys Lys 
            100                 105                 110         


Asn Arg Val Val Gln Ser Gly Ile Lys Val Arg Leu Gln Leu Tyr Arg 
        115                 120                 125             


Thr Ala Lys Met Gly Trp Gly Val Arg Ala Leu Gln Thr Ile Pro Gln 
    130                 135                 140                 


Gly Thr Phe Ile Cys Glu Tyr Val Gly Glu Leu Ile Ser Asp Ala Glu 
145                 150                 155                 160 


Ala Asp Val Arg Glu Asp Asp Ser Tyr Leu Phe Asp Leu Asp Asn Lys 
                165                 170                 175     


Asp Gly Glu Val Tyr Cys Ile Asp Ala Arg Tyr Tyr Gly Asn Ile Ser 
            180                 185                 190         


Arg Phe Ile Asn His Leu Cys Asp Pro Asn Ile Ile Pro Val Arg Val 
        195                 200                 205             


Phe Met Leu His Gln Asp Leu Arg Phe Pro Arg Ile Ala Phe Phe Ser 
    210                 215                 220                 


Ser Arg Asp Ile Arg Thr Gly Glu Glu Leu Gly Phe Asp Tyr Gly Asp 
225                 230                 235                 240 


Arg Phe Trp Asp Ile Lys Ser Lys Tyr Phe Thr Cys Gln Cys Gly Ser 
                245                 250                 255     


Glu Lys Cys Lys His Ser Ala Glu Ala Ile Ala Leu Glu Gln Ser Arg 
            260                 265                 270         


Leu Ala Arg Leu Asp Leu Ser Phe Glu Leu Leu Glu Ser Ser Asn Ser 
        275                 280                 285             


Leu Ala Val Val Leu Gln Arg Arg Asp Trp Glu Asn Pro Gly Val Thr 
    290                 295                 300                 


Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp Arg Asn 
305                 310                 315                 320 


Ser Glu Glu Ala Arg Thr Asp Arg 
                325             


<210> 18
<211> 559
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 18
Met Trp Glu Leu Val Ala Leu Leu Leu Leu Thr Leu Ala Tyr Leu Phe 
1               5                   10                  15      


Trp Pro Lys Arg Arg Cys Pro Gly Ala Lys Tyr Pro Lys Ser Leu Leu 
            20                  25                  30          


Ser Leu Pro Leu Val Gly Ser Leu Pro Phe Leu Pro Arg His Gly His 
        35                  40                  45              


Met His Asn Asn Phe Phe Lys Leu Gln Lys Lys Tyr Gly Pro Ile Tyr 
    50                  55                  60                  


Ser Val Arg Met Gly Thr Lys Thr Thr Val Ile Val Gly His His Gln 
65                  70                  75                  80  


Leu Ala Lys Glu Val Leu Ile Lys Lys Gly Lys Asp Phe Ser Gly Arg 
                85                  90                  95      


Pro Gln Met Ala Thr Leu Asp Ile Ala Ser Asn Asn Arg Lys Gly Ile 
            100                 105                 110         


Ala Phe Ala Asp Ser Gly Ala His Trp Gln Leu His Arg Arg Leu Ala 
        115                 120                 125             


Met Ala Thr Phe Ala Leu Phe Lys Asp Gly Asp Gln Lys Leu Glu Lys 
    130                 135                 140                 


Ile Ile Cys Gln Glu Ile Ser Thr Leu Cys Asp Met Leu Ala Thr His 
145                 150                 155                 160 


Asn Gly Gln Ser Ile Asp Ile Ser Phe Pro Val Phe Val Ala Val Thr 
                165                 170                 175     


Asn Val Ile Ser Leu Ile Cys Phe Asn Thr Ser Tyr Lys Asn Gly Asp 
            180                 185                 190         


Pro Glu Leu Asn Val Ile Gln Asn Tyr Asn Glu Gly Ile Ile Asp Asn 
        195                 200                 205             


Leu Ser Lys Asp Ser Leu Val Asp Leu Val Pro Trp Leu Lys Ile Phe 
    210                 215                 220                 


Pro Asn Lys Thr Leu Glu Lys Leu Lys Ser His Val Lys Ile Arg Asn 
225                 230                 235                 240 


Asp Leu Leu Asn Lys Ile Leu Glu Asn Tyr Lys Glu Lys Phe Arg Ser 
                245                 250                 255     


Asp Ser Ile Thr Asn Met Leu Asp Thr Leu Met Gln Ala Lys Met Asn 
            260                 265                 270         


Ser Asp Asn Gly Asn Ala Gly Pro Asp Gln Asp Ser Glu Leu Leu Ser 
        275                 280                 285             


Asp Asn His Ile Leu Thr Thr Ile Gly Asp Ile Phe Gly Ala Gly Val 
    290                 295                 300                 


Glu Thr Thr Thr Ser Val Val Lys Trp Thr Leu Ala Phe Leu Leu His 
305                 310                 315                 320 


Asn Pro Gln Val Lys Lys Lys Leu Tyr Glu Glu Ile Asp Gln Asn Val 
                325                 330                 335     


Gly Phe Ser Arg Thr Pro Thr Ile Ser Asp Arg Asn Arg Leu Leu Leu 
            340                 345                 350         


Leu Glu Ala Thr Ile Arg Glu Val Leu Arg Leu Arg Pro Val Ala Pro 
        355                 360                 365             


Met Leu Ile Pro His Lys Ala Asn Val Asp Ser Ser Ile Gly Glu Phe 
    370                 375                 380                 


Ala Val Asp Lys Gly Thr Glu Val Ile Ile Asn Leu Trp Ala Leu His 
385                 390                 395                 400 


His Asn Glu Lys Glu Trp His Gln Pro Asp Gln Phe Met Pro Glu Arg 
                405                 410                 415     


Phe Leu Asn Pro Ala Gly Thr Gln Leu Ile Ser Pro Ser Val Ser Tyr 
            420                 425                 430         


Leu Pro Phe Gly Ala Gly Pro Arg Ser Cys Ile Gly Glu Ile Leu Ala 
        435                 440                 445             


Arg Gln Glu Leu Phe Leu Ile Met Ala Trp Leu Leu Gln Arg Phe Asp 
    450                 455                 460                 


Leu Glu Val Pro Asp Asp Gly Gln Leu Pro Ser Leu Glu Gly Ile Pro 
465                 470                 475                 480 


Lys Val Val Phe Leu Ile Asp Ser Phe Lys Val Lys Ile Lys Val Arg 
                485                 490                 495     


Gln Ala Trp Arg Glu Ala Gln Ala Glu Gly Ser Thr Ser Ser Phe Glu 
            500                 505                 510         


Leu Leu Glu Ser Ser Asn Ser Leu Ala Val Val Leu Gln Arg Arg Asp 
        515                 520                 525             


Trp Glu Asn Pro Gly Val Thr Gln Leu Asn Arg Leu Ala Ala His Pro 
    530                 535                 540                 


Pro Phe Ala Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg 
545                 550                 555                 


<210> 19
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 19
His His His His His His 
1               5       


<210> 20
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polyhistidine tag


<220>
<221> misc_feature
<222> (1)..(10)
<223> This sequence may encompass 5-10 residues

<400> 20
His His His His His His His His His His 
1               5                   10  



