                         SEQUENCE LISTING

<110>  Lanzatech New Zealand Limited
       Koepke, Michael
       Chen, Wendy  Y
       Nagaraju, Shilpa
 
<120>  Recombinant Microorganisms and Methods of Use Thereof

<130>  LT74PCT

<150>  US 61/593269
<151>  2012-01-31

<160>  156   

<170>  PatentIn version 3.5

<210>  1
<211>  719
<212>  DNA
<213>  Clostridia autoethanogenum

<400>  1
atggatgatg aggtgaaagt cccaaaccat atatatcaaa tgtctacaat aaatgcactt       60

gtttcggggc tgtatgatgg ctgtgtttca ttatctaaac ttcttaaaaa aggaaacttt      120

ggtataggta cttttaaagg tctagatggt gaactaactc ttttaaatgg aactttttat      180

aggactaaac ctgatggcag cgtatacgta tgttccaaaa acgtatccgt tccttttgct      240

gtagtcactg aactggaaaa ttataatact tataatattc aaaatcgtac ttcttatgaa      300

gatataagaa aagaattgga cagctttata gaaagcaaaa atatatttta tgctttctat      360

atggaaggta aatttaatta tgtaaaaaca cgtactgttg taaaacagaa tatgccttat      420

aagcctatgg ctgaagttgt taaagatcag cctatgtttg aatataacgg tgttgatgga      480

tatgtggttg gatttaggtg tcctgattat gttgaaggcc ttaatgtccc tggatatcat      540

tttcatttca taaataaaga taagaaattt ggtggacata taagtgaatt ttccattgaa      600

aatgcgaagg tttatgtaca gaactgttct tgctttagga tggaacttcc taaaaagaaa      660

gtttttataa tatggaagta caagatagaa acgatgagat aacaagtgtt gaaaaataa       719


<210>  2
<211>  239
<212>  PRT
<213>  Clostridium autoethanogenum

<400>  2

Met Asp Asp Glu Val Lys Val Pro Asn His Ile Tyr Gln Met Ser Thr 
1               5                   10                  15      


Ile Asn Ala Leu Val Ser Gly Leu Tyr Asp Gly Cys Val Ser Leu Ser 
            20                  25                  30          


Lys Leu Leu Lys Lys Gly Asn Phe Gly Ile Gly Thr Phe Lys Gly Leu 
        35                  40                  45              


Asp Gly Glu Leu Thr Leu Leu Asn Gly Thr Phe Tyr Arg Thr Lys Pro 
    50                  55                  60                  


Asp Gly Ser Val Tyr Val Cys Ser Lys Asn Val Ser Val Pro Phe Ala 
65                  70                  75                  80  


Val Val Thr Glu Leu Glu Asn Tyr Asn Thr Tyr Asn Ile Gln Asn Arg 
                85                  90                  95      


Thr Ser Tyr Glu Asp Ile Arg Lys Glu Leu Asp Ser Phe Ile Glu Ser 
            100                 105                 110         


Lys Asn Ile Phe Tyr Ala Phe Tyr Met Glu Gly Lys Phe Asn Tyr Val 
        115                 120                 125             


Lys Thr Arg Thr Val Val Lys Gln Asn Met Pro Tyr Lys Pro Met Ala 
    130                 135                 140                 


Glu Val Val Lys Asp Gln Pro Met Phe Glu Tyr Asn Gly Val Asp Gly 
145                 150                 155                 160 


Tyr Val Val Gly Phe Arg Cys Pro Asp Tyr Val Glu Gly Leu Asn Val 
                165                 170                 175     


Pro Gly Tyr His Phe His Phe Ile Asn Lys Asp Lys Lys Phe Gly Gly 
            180                 185                 190         


His Ile Ser Glu Phe Ser Ile Glu Asn Ala Lys Val Tyr Val Gln Asn 
        195                 200                 205             


Cys Ser Cys Phe Arg Met Glu Leu Pro Lys Asn Glu Ser Phe Tyr Asn 
    210                 215                 220                 


Met Glu Val Gln Asp Arg Asn Asp Glu Ile Thr Ser Val Glu Lys 
225                 230                 235                 


<210>  3
<211>  964
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  3
tttcttcaca ggaaaatata cttcagtaac aagatcttta ggaatggtga cttggtgggg       60

gtcagttaca tatacttcat atggtgggtt tgtaagttta tatccttcat tttctaccca      120

ttccctcaac ttagcatata cagagatgtt aattctgaat atgagcccct taaaacagac      180

ttcgcacaaa ggactccagg caagtatctt gttcccttta caatctcctt tatcggaatg      240

gcaagttctg tatcattgcc agaaggattg tattcagcgc tgtgataaat agttattggc      300

ttaccaagaa agtcaattac aaaaatatat ataaagaaag caaagctaca tatattaaag      360

catttaaggt aaaactaaaa atattataaa aatgaaatta ttttttctca tagctaaagt      420

tacataatac gaggaggatt tataatgaaa aaagtaatag gaattataag tattgtacta      480

tttgtactcg tagcacttca atcctgtgct gcaggagtag gaaatgcatt aagtaataac      540

aaagaagcta gtggatctgc tggattattt ttatctgtat gtatgcttat tgctggaata      600

atagcaataa tatcaaaata tagtaaaggt atgactataa cagctatagt attttatttg      660

ttagcttttg ttgtagggat tgctaatgtt gggcattttt cagatttgca aatttggtca      720

atcattaact tgatatttgc tggactattg atatttcatt tgcttaaaaa taagcaatta      780

tataatagca gtgggaaaaa gtagaatcat atattgtaat tatttttaat tatgttggca      840

aaattgaaat tgtcactgaa acacctctaa atgttttaaa tacatatgtt taattattgt      900

gacagattct aatagtagaa agtagaaatt tgctatgtta taatgacata gaggtgaatg      960

taat                                                                   964


<210>  4
<211>  977
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  4
actagacagt gctaataaca atgtctagtg ctttttatct tgctcaattt tttcattgag       60

ttcatttaag taagtccacc tgtccatctt ttcgtctagc tctttttcca gtgaattctt      120

ttcggataag agatcttcaa gaagtgcata atcagatgaa gcagcttcca tttctatttt      180

cttttcagat atagattttt ctagatgttc aattacctca tctattttgt caaactccat      240

ttgttctgca taggtaaatt ttagaggctt ttctttttgc aacttatagt tgtttttagc      300

tgtatttttc ttagagctta ttttttcctc tgatattttt gcagttttgt gaaaatagga      360

atagtttcct gtatattgag tgattttacc gtttccttca aaagaaaata ttttatcaac      420

tgttttgtca aggaagtacc tgtcatgaga tacagctata acagctcctt caaaatcgtt      480

aatataatct tctaggattg taagtgtttc tatatccaga tcatttgttg gttcgtccag      540

caaaagtaca ttagggtaat tcatcagtat ttttagaaga tataatcttc ttcgttctcc      600

tcctgaaagt tttccaaggg gagtccattg aactgaaggt tcaaataaaa aattttcaag      660

tacagcagaa gcacttattt tttcacccga tgaagttgac gcatattctg atgtcccacg      720

tatgtattca attacccttt cgttcatatc catatcagaa attccctgag aatagtatcc      780

tatctttact gtttcaccta tatctatagt gccgctgtcc ggcagaattt tttgaactaa      840

aatattcata agagtggatt taccacttcc attaggtcca ataataccta ttctgtcatt      900

atttagtatg ttataagtga aatttttaat taatgtcttt tcaccaaaac ttttgcttat      960

gttatccagg tttatga                                                     977


<210>  5
<211>  40
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  5
attcatcctg caggtttctt cacaggaaaa tatacttcag                             40


<210>  6
<211>  40
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  6
gactgcggcc gcattacatt cacctctatg tcattataac                             40


<210>  7
<211>  38
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  7
atttgctagc actagacagt gctaataaca atgtctag                               38


<210>  8
<211>  35
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  8
atatggcgcg cctcataaac ctggataaca taagc                                  35


<210>  9
<211>  2963
<212>  DNA
<213>  Escherichia coli

<400>  9
cctgcaggat aaaaaaattg tagataaatt ttataaaata gttttatcta caattttttt       60

atcaggaaac agctatgacc gcggccgctg tatccatatg accatgatta cgaattcgag      120

ctcggtaccc ggggatcctc tagagtcgac gtcacgcgtc catggagatc tcgaggcctg      180

cagacatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg      240

cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga      300

agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcta      360

gcataaaaat aagaagcctg catttgcagg cttcttattt ttatggcgcg ccgcattcac      420

ttcttttcta tataaatatg agcgaagcga ataagcgtcg gaaaagcagc aaaaagtttc      480

ctttttgctg ttggagcatg ggggttcagg gggtgcagta tctgacgtca atgccgagcg      540

aaagcgagcc gaagggtagc atttacgtta gataaccccc tgatatgctc cgacgcttta      600

tatagaaaag aagattcaac taggtaaaat cttaatatag gttgagatga taaggtttat      660

aaggaatttg tttgttctaa tttttcactc attttgttct aatttctttt aacaaatgtt      720

cttttttttt tagaacagtt atgatatagt tagaatagtt taaaataagg agtgagaaaa      780

agatgaaaga aagatatgga acagtctata aaggctctca gaggctcata gacgaagaaa      840

gtggagaagt catagaggta gacaagttat accgtaaaca aacgtctggt aacttcgtaa      900

aggcatatat agtgcaatta ataagtatgt tagatatgat tggcggaaaa aaacttaaaa      960

tcgttaacta tatcctagat aatgtccact taagtaacaa tacaatgata gctacaacaa     1020

gagaaatagc aaaagctaca ggaacaagtc tacaaacagt aataacaaca cttaaaatct     1080

tagaagaagg aaatattata aaaagaaaaa ctggagtatt aatgttaaac cctgaactac     1140

taatgagagg cgacgaccaa aaacaaaaat acctcttact cgaatttggg aactttgagc     1200

aagaggcaaa tgaaatagat tgacctccca ataacaccac gtagttattg ggaggtcaat     1260

ctatgaaatg cgattaaggg ccggccagtg ggcaagttga aaaattcaca aaaatgtggt     1320

ataatatctt tgttcattag agcgataaac ttgaatttga gagggaactt agatggtatt     1380

tgaaaaaatt gataaaaata gttggaacag aaaagagtat tttgaccact actttgcaag     1440

tgtaccttgt acctacagca tgaccgttaa agtggatatc acacaaataa aggaaaaggg     1500

aatgaaacta tatcctgcaa tgctttatta tattgcaatg attgtaaacc gccattcaga     1560

gtttaggacg gcaatcaatc aagatggtga attggggata tatgatgaga tgataccaag     1620

ctatacaata tttcacaatg atactgaaac attttccagc ctttggactg agtgtaagtc     1680

tgactttaaa tcatttttag cagattatga aagtgatacg caacggtatg gaaacaatca     1740

tagaatggaa ggaaagccaa atgctccgga aaacattttt aatgtatcta tgataccgtg     1800

gtcaaccttc gatggcttta atctgaattt gcagaaagga tatgattatt tgattcctat     1860

ttttactatg gggaaatatt ataaagaaga taacaaaatt atacttcctt tggcaattca     1920

agttcatcac gcagtatgtg acggatttca catttgccgt tttgtaaacg aattgcagga     1980

attgataaat agttaacttc aggtttgtct gtaactaaaa acaagtattt aagcaaaaac     2040

atcgtagaaa tacggtgttt tttgttaccc taagtttaaa ctcctttttg ataatctcat     2100

gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat     2160

caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa     2220

accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa     2280

ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt     2340

aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt     2400

accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata     2460

gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt     2520

ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac     2580

gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga     2640

gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg     2700

ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa     2760

aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat     2820

gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc     2880

tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga     2940

agagcgccca atacgcaggg ccc                                             2963


<210>  10
<211>  16
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  10
gtaaaacgac ggccag                                                       16


<210>  11
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  11
caggaaacag ctatgacc                                                     18


<210>  12
<211>  4819
<212>  DNA
<213>  Artificial sequence

<220>
<223>  budA gene knockout plasmid pMTL85141-budA-ko

<400>  12
cctgcaggtt tcttcacagg aaaatatact tcagtaacaa gatctttagg aatggtgact       60

tggtgggggt cagttacata tacttcatat ggtgggtttg taagtttata tccttcattt      120

tctacccatt ccctcaactt agcatataca gagatgttaa ttctgaatat gagcccctta      180

aaacagactt cgcacaaagg actccaggca agtatcttgt tccctttaca atctccttta      240

tcggaatggc aagttctgta tcattgccag aaggattgta ttcagcgctg tgataaatag      300

ttattggctt accaagaaag tcaattacaa aaatatatat aaagaaagca aagctacata      360

tattaaagca tttaaggtaa aactaaaaat attataaaaa tgaaattatt ttttctcata      420

gctaaagtta cataatacga ggaggattta taatgaaaaa agtaatagga attataagta      480

ttgtactatt tgtactcgta gcacttcaat cctgtgctgc aggagtagga aatgcattaa      540

gtaataacaa agaagctagt ggatctgctg gattattttt atctgtatgt atgcttattg      600

ctggaataat agcaataata tcaaaatata gtaaaggtat gactataaca gctatagtat      660

tttatttgtt agcttttgtt gtagggattg ctaatgttgg gcatttttca gatttgcaaa      720

tttggtcaat cattaacttg atatttgctg gactattgat atttcatttg cttaaaaata      780

agcaattata taatagcagt gggaaaaagt agaatcatat attgtaatta tttttaatta      840

tgttggcaaa attgaaattg tcactgaaac acctctaaat gttttaaata catatgttta      900

attattgtga cagattctaa tagtagaaag tagaaatttg ctatgttata atgacataga      960

ggtgaatgta atgcggccgc tgtatccata tgaccatgat tacgaattcg agctcggtac     1020

ccggggatcc tctagagtcg acgtcacgcg tccatggaga tctcgaggcc tgcagacatg     1080

caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc     1140

aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc     1200

gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tagcagtatt     1260

gatagaaaaa aacactagac agtgctaata acaatgtcta gtgcttttta tcttgctcaa     1320

ttttttcatt gagttcattt aagtaagtcc acctgtccat cttttcgtct agctcttttt     1380

ccagtgaatt cttttcggat aagagatctt caagaagtgc ataatcagat gaagcagctt     1440

ccatttctat tttcttttca gatatagatt tttctagatg ttcaattacc tcatctattt     1500

tgtcaaactc catttgttct gcataggtaa attttagagg cttttctttt tgcaacttat     1560

agttgttttt agctgtattt ttcttagagc ttattttttc ctctgatatt tttgcagttt     1620

tgtgaaaata ggaatagttt cctgtatatt gagtgatttt accgtttcct tcaaaagaaa     1680

atattttatc aactgttttg tcaaggaagt acctgtcatg agatacagct ataacagctc     1740

cttcaaaatc gttaatataa tcttctagga ttgtaagtgt ttctatatcc agatcatttg     1800

ttggttcgtc cagcaaaagt acattagggt aattcatcag tatttttaga agatataatc     1860

ttcttcgttc tcctcctgaa agttttccaa ggggagtcca ttgaactgaa ggttcaaata     1920

aaaaattttc aagtacagca gaagcactta ttttttcacc cgatgaagtt gacgcatatt     1980

ctgatgtccc acgtatgtat tcaattaccc tttcgttcat atccatatca gaaattccct     2040

gagaatagta tcctatcttt actgtttcac ctatatctat agtgccgctg tccggcagaa     2100

ttttttgaac taaaatattc ataagagtgg atttaccact tccattaggt ccaataatac     2160

ctattctgtc attatttagt atgttataag tgaaattttt aattaatgtc ttttcaccaa     2220

aacttttgct tatgttatcc aggtttatga cttttttacc ggcgcgccgc attcacttct     2280

tttctatata aatatgagcg aagcgaataa gcgtcggaaa agcagcaaaa agtttccttt     2340

ttgctgttgg agcatggggg ttcagggggt gcagtatctg acgtcaatgc cgagcgaaag     2400

cgagccgaag ggtagcattt acgttagata accccctgat atgctccgac gctttatata     2460

gaaaagaaga ttcaactagg taaaatctta atataggttg agatgataag gtttataagg     2520

aatttgtttg ttctaatttt tcactcattt tgttctaatt tcttttaaca aatgttcttt     2580

tttttttaga acagttatga tatagttaga atagtttaaa ataaggagtg agaaaaagat     2640

gaaagaaaga tatggaacag tctataaagg ctctcagagg ctcatagacg aagaaagtgg     2700

agaagtcata gaggtagaca agttataccg taaacaaacg tctggtaact tcgtaaaggc     2760

atatatagtg caattaataa gtatgttaga tatgattggc ggaaaaaaac ttaaaatcgt     2820

taactatatc ctagataatg tccacttaag taacaataca atgatagcta caacaagaga     2880

aatagcaaaa gctacaggaa caagtctaca aacagtaata acaacactta aaatcttaga     2940

agaaggaaat attataaaaa gaaaaactgg agtattaatg ttaaaccctg aactactaat     3000

gagaggcgac gaccaaaaac aaaaatacct cttactcgaa tttgggaact ttgagcaaga     3060

ggcaaatgaa atagattgac ctcccaataa caccacgtag ttattgggag gtcaatctat     3120

gaaatgcgat taagggccgg ccagtgggca agttgaaaaa ttcacaaaaa tgtggtataa     3180

tatctttgtt cattagagcg ataaacttga atttgagagg gaacttagat ggtatttgaa     3240

aaaattgata aaaatagttg gaacagaaaa gagtattttg accactactt tgcaagtgta     3300

ccttgtacct acagcatgac cgttaaagtg gatatcacac aaataaagga aaagggaatg     3360

aaactatatc ctgcaatgct ttattatatt gcaatgattg taaaccgcca ttcagagttt     3420

aggacggcaa tcaatcaaga tggtgaattg gggatatatg atgagatgat accaagctat     3480

acaatatttc acaatgatac tgaaacattt tccagccttt ggactgagtg taagtctgac     3540

tttaaatcat ttttagcaga ttatgaaagt gatacgcaac ggtatggaaa caatcataga     3600

atggaaggaa agccaaatgc tccggaaaac atttttaatg tatctatgat accgtggtca     3660

accttcgatg gctttaatct gaatttgcag aaaggatatg attatttgat tcctattttt     3720

actatgggga aatattataa agaagataac aaaattatac ttcctttggc aattcaagtt     3780

catcacgcag tatgtgacgg atttcacatt tgccgttttg taaacgaatt gcaggaattg     3840

ataaatagtt aacttcaggt ttgtctgtaa ctaaaaacaa gtatttaagc aaaaacatcg     3900

tagaaatacg gtgttttttg ttaccctaag tttaaactcc tttttgataa tctcatgacc     3960

aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa     4020

ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca     4080

ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta     4140

actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc     4200

caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca     4260

gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta     4320

ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag     4380

cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt     4440

cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc     4500

acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac     4560

ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac     4620

gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc     4680

tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat     4740

accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag     4800

cgcccaatac gcagggccc                                                  4819


<210>  13
<211>  1460
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  13
ggctcaggac gaacgctggc ggcgtgctta acacatgcaa gtcgagcgat gaagctcctt       60

cgggagtgga ttagcggcgg acgggtgagt aacacgtggg taacctacct caaagagggg      120

gatagcctcc cgaaagggag attaataccg cataataatc agttttcaca tggagactga      180

tttaaaggag taatccgctt tgagatggac ccgcggcgca ttagctagtt ggtagggtaa      240

cggcctacca aggcgacgat gcgtagccga cctgagaggg tgatcggcca cattggaact      300

gagagacggt ccagactcct acgggaggca gcagtgggga atattgcaca atgggcgaaa      360

gcctgatgca gcaacgccgc gtgagtgaag aaggttttcg gattgtaaag ctctgtcttt      420

ggggacgata atgacggtac ccaaggagga agccacggct aactacgtgc cagcagccgc      480

ggtaatacgt aggtggcgag cgttgtccgg aattactggg cgtaaagagt gcgtaggcgg      540

atatttaagt gagatgtgaa atacccgggc ttaacccggg cactgcattt caaactggat      600

atctagagtg cgggagagga gaatggaatt cctagtgtag cggtgaaatg cgtagagatt      660

aggaagaaca ccagtggcga aggcgattct ctggaccgta actgacgctg aggcacgaaa      720

gcgtgggtag caaacaggat tagataccct ggtagtccac gccgtaaacg atgagtacta      780

ggtgtaggag gtatcgaccc cttctgtgcc gcagtaaaca caataagtac tccgcctggg      840

aagtacgatc gcaagattaa aactcaaagg aattgacggg ggcccgcaca agcagcggag      900

catgtggttt aattcgaagc aacgcgaaga accttacctg gacttgacat accctgaata      960

tcttagagat aagagaagcc cttcggggca gggatacagg tggtgcatgg ttgtcgtcag     1020

ctcgtgtcgt gagatgttag gttaagtcct gcaacgagcg caacccctgt tgttagttgc     1080

taacatttag ttgagcactc tagcaagact gccgcggtta acgcggagga aggtggggat     1140

gacgtcaaat catcatgccc cttatgtcca gggcaacaca cgtgctacaa tgggcagtac     1200

agagagaagc aagaccgcaa ggtggagcaa acctcaaaaa ctgcccccag ttcggattgc     1260

aggctgaaac tcgcctacat gaagttggag ttgctagtaa tcgcgaatca gaatgtcgcg     1320

gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccatgagagc tggcaacacc     1380

cgaagtccgt agtctaactt aggaggacgc ggccgaaggt ggggttagta attggggtga     1440

agtcgtaaca aggtagccgt                                                 1460


<210>  14
<211>  677
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  14
aagaacmttg saaaktccst racsatggwg asycstwmgc kkagkrrmyy mgcrrysgac       60

gggtgagtma cacgtgggta acctaccycr rrgaggggga tagcctcccs aaagggagat      120

taataccgca taataatcag ttttcacatg gagaytgwtt taaaggagta atccgctttg      180

agatggaccc gcggcgcatt agctagttgg tagggtaacg gcctaccaag gcgackatgc      240

gtagccgacc tgagagggtg atcggccaca ttggaactga sagacggtcc asactcctac      300

gggaggcagc agtggggaat attgcacaat gggcgaaagc ctgatgcagc aacgccgcgt      360

gagtgaagaa ggttttcgga ttgtaaagct ctgtctttgg ggacgataat gacggtaccc      420

aaggaggaag ccacggstaa ctacgkgcca scakccgcgg taatacgtas gtggcgagcg      480

ttgtccggaa ttactgggcg taaakastgc gtakgcggat atttaaktga satgtgaaat      540

asccgggctt aaccygggyw ctgywtttca mactggatat ctakagtgcg ggagaggasa      600

atgkaattcy taktgtascg gtgaartgcs takasattak gaasaacacc mktggcgaak      660

gcgattckct ggaccgt                                                     677


<210>  15
<211>  720
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  15
trarakkgak cyysgrtccc kkgrmswcst ggyarggtaa csgymwrcyw rgrysacgak       60

gcgtmgycra cctgaraggg tgatcggcca cmttggaact gagagacggt ccaractcct      120

acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc      180

gtgagtgaag aaggttttcg gattgtaaag ctctgtcttt ggggacgata atgacggtmc      240

ccaaggagga agccacggct aactacgtgc cascagccgc ggtaatacgt aggtggcrag      300

cgttgtccgg aattactggg cgtaaagagt gcgtaggcgg atatttaagt gagatgtgaa      360

atacccgggc ttaacccggg cactgcwttt caaactggat atctakagtg cgggagagga      420

gaatgkaatt cctagtgtag cggtgaaatg cgtakagatt aggaagaaca ccmgtggcga      480

akgcgattct ctggaccgta actgayrctg akgcacgaag cgtggggtak cawacakgat      540

tagatacyct ggtrstccac rccgtaaacg atgagtayta kgtgtakgag kwtcsacccc      600

cttctgtgcc ssmmtaraca ymmyaaktac tcccgcckcr aagtmsawcg cmagatkaaa      660

amtcrwmgsa rtkrwggggg ggcsgcmcta acatcgsast wrkwkkttsr attawarcaa      720


<210>  16
<211>  960
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  16
taaaggagta atccgytttg agatggaccc gcggcgcatt agctwgttgg tagggtaacg       60

gcctaccmwg gcgackatgc gtagccgacc tgagagggtg atcggccaca ttggaactga      120

gagacggtcc aractcctac gggaggcagc agtggggaat attgcacaat gggcgaaagc      180

ctgatgcagc aacgccgcgt gagtgaagaa ggttttcgga ttgtaaagct ctgtctttgg      240

ggacgataat gacggtaccc aaggaggaag ccacggstaa ctacgtgcca scagccgcgg      300

taatacgtag gtggcgagcg ttgtccggaa ttactgggcg taaagagtgc gtaggcggat      360

atttaagtga gatgtgaaat acccgggctt aacccgggyw ctgcatttca aactggatat      420

ctagagtgcg ggagaggaga atggaattcc tagtgtagcg gtgaartgcg takagattak      480

gaagaacacc agtggcgaag gcgattctct ggaccgtrac tgacgctgag gcacgaaagc      540

gtgggtagca aacaggatta gataccctgg tagtccacrc cgtaaacgat gagtactakg      600

tgtaggaggt atcgacccct tctgtgccgc agtaaacaca ataagtacty ckcctgggaa      660

gtacgatcgc aagattaaaa ctcaasgaak tgacaggsgc ccgcacwagc akcgasyatg      720

tggtttattc gaagcacgcg aagaacctta cctggacttg acataccctg mwatctwtas      780

ataagagagc scttcgggtc aggatrcagt cgtgcatggt gtcgtcwgct cgtgtcrtga      840

gatgtagtar tctgcaacsa kcgyacyctg tggyagtgct acatgmtsag cmtctagcag      900

actgcgmgta sccgsagagy ggggatgacg tcgakcatca tgycctyagt cmcgyctacr      960


<210>  17
<211>  676
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  17
atttkggsar aktccgkakg caaggtgasc cgtargcttg gatccyggga akksryrgsw       60

grgtamcysk kgkstwrswt mccssgraga rggggawags ctcccgaaag ggagattamt      120

accgcataat aatcagtttt cacatggaga ctgatttaaa ggagtaatcc gctttgagat      180

ggacccgcgg cgcattagct agttggtagg gtaacggcct accaaggcga ckatgcgtag      240

ccgacctgag agggtgatcg gccacwttgg aactgagaga cggtccasac tcctacggga      300

ggcagcagtg gggaatattg cacaatgggc gaaagcctga tgcagcaacg ccgcgtgagt      360

gaagaaggtt ttcggattgt aaagctctgt ctttggggac gataatgacg gtacccmasg      420

aggargccmc ggsyaactac gkgccwscmk ccgcggtaat acrtaggtgg cragcgttgt      480

ccggaattac tgggcgtaaa kagtgcgtak gcggatattt aaktgagatg tgaaryascc      540

gggcttaacc cgggcwctgy atttcwmayt ggatatctmk agtgcgggrg aggagaatgg      600

awgtyctakk gtamcsgtga artgcstaka satwmkgmas aacaycwstg gcgwarrcgr      660

ytcgswggac cgtawc                                                      676


<210>  18
<211>  1040
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  18
tagsaaaktc ytakrcaatg gtarycstwa gcttgkatrm krmwmgrsgg acgggtgagt       60

aacmckkggg taamctacyt crragarggg gatagcctcc csaaagggag attaataccg      120

cataataatc agttttcaca tggagactga tttaaaggag taatccgctt tgagatggac      180

ccgcggcgca ttagctagtt ggtagggtaa cggcctacca aggcgackat gcgtagccga      240

cctgagaggg tgatcggcca cattggaact gagagacggt ccagactcct acgggaggca      300

gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc gtgagtgaag      360

aaggttttcg gattgtaaag ctctgtcttt ggggacgata atgacggtac ccaaggagga      420

rgccacggst aactacgkgc cascmkccgc ggtaatacgt asgtggcgag cgttgtccgg      480

aattactggg cgtaaagagt gcgtakgcgg atatttaagt gagatgtgaa atasccggsc      540

ttaacccggg cactgcattt camactggat atctakagtg cgggagagga saatgkratt      600

cctakkgtas cggtgaaatg cstatasatt akgaasaaca ccmktgkcga akgcgawtck      660

ctggaccrtr rctgacrcts akgcaygywa gcstsgstwk cwwrcmksat yatatacccy      720

ggkrgtcmcr wcrymwmcat sagtactakg tgtmkkaggt atckmcmcct yctytgcssc      780

mkwaraamaa yawkmwcytc csccysssgr rkwacaawcr mwakatkaat agwmatggsa      840

kkkamggssg gccsccswma catcysmkct rwtrktkwat ttcaykcamk ymmsmaamka      900

acctgkmytg rsmtasccyg cycysswwtw awctaagmam agcmtcscss tamgrgwkmr      960

gwsrygsstk ygytsrtggc tmtcgtcayy tmgsrymgar aratratwst awacsmwsms     1020

aamccmykyc ywycctkstk                                                 1040


<210>  19
<211>  674
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  19
ggggrtakcc tcccsaawgg garaytaatw ccgcataata atcagttttc acatggagac       60

tgatttaaag gagtaatccg ctttgagatg gacccgcggc gcattagcta gttggtaggg      120

taacggccta ccaaggcgac gatgcgtagc cgacctgaga gggtgatcgg scacattgga      180

actgagagac ggtccaract cctacgggag gcagcagtgg ggaatattgc acaatgggcg      240

aaagcctgat gcagcaacgc cgcgtgagtg aagaaggttt tcggattgta aagctctgtc      300

tttggggacg atratgacgg tacccaagga ggaagccacg gstaactacg tgccascakc      360

cgcggtaata cgtaggtggc gagcgttgtc cggaattact gggcgtaaak agtgcgtarg      420

cggatattta agtgagatgt gaaatasccg gscttaaccc gggcwctgca tttcwaactg      480

gatatctaka gtgcgggaga ggagwatkta wttcctagtg trscggtgaa atgsgkasam      540

atyakgmaga acmccagtgk cgaaggcgay tckstggacc ryractgamg ctsawgcwcg      600

maagcgwgss tagcaaasat gattagatay mcyggtagwc mcamcrmmaa csatgagkac      660

trkgtgtmsk asgt                                                        674


<210>  20
<211>  1226
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  20
crgrwycttt mggatkgkwg acttgskggg ggtcagtwmc awatactkcm wawggwgggt       60

ttgtargttt atatccttca ttttctaccc attccctcra mtwakywwat wcaragatgt      120

taayyckraa tatgarcccc ttaaaacrga sttcsmacaa aggactccwg gyaakywtct      180

kgttcccttt acawtctcct twaysrraat ggmaakttct gyatcmttgc casawggatt      240

gwwttcascg ctkygwtaaa tagttattgg cttaccwmka aagtcmwtta caaaaatata      300

tataaagaaa gcaaagctac wkatwtyaaa ksattwaagg taaarmtaaa aatatwataa      360

aawwgaamtt attttttctc wtakstaawg ttacwtaata cgaggaggat ttataatgaa      420

aaaagtaata ggawttataa ryattgwmct atttgkactm ktagcacttc aatcctgtgc      480

kgcmkgakwa ggaartgymt yaagwaatra cmwwsawksw mgwgratctg cwggatwatt      540

tttatytkka tgkatgctka ttgctggaat aatakmmatr awaycawamy wwwktamagg      600

tatgacyata acagctatag katttwattt gttakctttt gttgyaggga twgctaaygw      660

tgggcatttt wcagatttgc awatttgrtc aaycwttaac twgatatttg ctggactatw      720

gatatttcat ttrctkaama wtaagmaatt atatwatakc agtggraaaa agwakaatca      780

tatrttgtaa ttatttttaa ttatgtkrrc aamwytgawa ttgwcacwga waacayctct      840

aaatgtttwr aatacatatg tttmaktakt gtgacakatw ctaatastak aaagwagaar      900

wtygctatrw watratgaca tagwggtgaa tgtaatgcsg mckctgwryc catatsacca      960

tgatrcgaat tmsagctsgg tacscsggrk atcctctrga stcgwcgtya ckcgtccatg     1020

kagatmwcga gcctggmgac atgcagctta gcwckggtcg tcatkttacw cgtcgtsact     1080

rsgtaaaacc atgacgtmcc rctgtcgcat gcwgcacrtc yccrtatcgt cagctrcgta     1140

wcgcgacyag ccgatcgatc gcctgccwcg atgccractg atgcatgcct gmcakacggc     1200

aywacaagtc taggcattac tggcca                                          1226


<210>  21
<211>  1236
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  21
twwttwwacm ttactaaata atgacagaat aggtattatt ggacctaatg gaagtggtaa       60

atccactctt atgaatattt tarttcaaaa aattctgccg gacagcggca ctatagatat      120

aggtgaaaca gtaaagatag gatactattc tcagggaatt tctgatatgg atatgaacga      180

aagggtaatt gaatacatac gtgggacatc rraatatgcg tcaacttcat cgggtgaaaa      240

aakaaktgct tctgctgtac ttgaaaattt tttatttgaa ccttcagttc aatggactcc      300

ccttggaaaa ctktcaggag gagaacgaar aagattatat cttctaaaaa tactgatgaa      360

ttaccctaat gtacttttgc tggacgaacc aacaaatgat ctggatatag aaacacttac      420

aatcctagaa gattatatta acgattttga aggagctgtt atagctgtat ctcatgacag      480

gtacttcctt gacaaaacag ttgataaaat attttctttt gaaggaaacg gtaaaatcac      540

tcaatataca ggaaactatt cctattttca caaaactgca aaaatatcag aggaaaaaat      600

aagctctaag aaaaatacag ctaaaaacaa ctatragttg caaaaagaaa agcctctaaa      660

atttacctat gcagaacaaa tggagtttga caaaatagat gaggtaattg aacatctaga      720

aaaatctata tctgaaaaga aaatagaaat ggaagctgct tcatctgatt atgcacttct      780

tgaagatctc ttatccgaaa agaattcact ggaaaaagag ctagacgaaa agatggacag      840

gtggacttac ttaatgaact caatgaaaaa attgagcaag ataaaagcac tagacattgt      900

tattagcact gtctagtgct agcgccattc gccattcatg ctgcgcaact gtgggaaggg      960

cgatcggtgc ggcctcttcg ctaytacgcc agctggcgaa gggatgtgct gcaagscgat     1020

aagttggtac gccaggtttc cagtcacgac gtagwaaacg acgtcagtgc tagctgcatg     1080

tctgcagctc gagattctca tggascgtka cgtcgacytr asgatcctgg tactrrctcg     1140

attcgtatcm tggwcawtgg atmgcggcgc atamctcccc tatgcattaa catgcaattc     1200

acgtctacta tagagtctgt tccaaaatra acgcgt                               1236


<210>  22
<211>  1224
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  22
gawtttttcm acttttataa catactaaat aatgacagaa taggtattat tggacctaat       60

ggaagtggta aatccactct tatgaatatt ttarttcaaa aaattctgcc ggacagcggc      120

actatagata taggtgaaac agtaaagata ggatactatt ctcagggaat ttctgatatg      180

gatatgaacg aaagggtaat tgaatacata cgtgggacat caraatatgc gtcaacttca      240

tcgggtgaaa aaataagtgc ttctgctgta cttgaaaatt ttttatttga accttcagtt      300

caatggactc cccttggaaa actttcagga ggagaacgaa raagattata tcttctaaaa      360

atactgatga attaccctaa tgtacttttg ctggacgaac caacaaatga tctggatata      420

gaaacactta caatcctaga agattatatt aacgattttg aaggagctgt tatagctgta      480

tctcatgaca ggtacttcct tgacaaaaca gttgataaaa tattttcttt tgaaggaaac      540

ggtaaaatca ctcaatatac aggaaactat tcctattttc acaaaactgc aaaaatatca      600

gaggaaaaaa taagctctaa gaaaaataca gctaaaaaca actataagtt gcaaaaagaa      660

aagcctctaa aatttaccta tgcagaacaa atggagtttg acaaaataga tgaggtaatt      720

gaacatctag aaaaatctat atctgaaaag aaaatagaaa tggaagctgc ttcatctgat      780

tatgcacttc ttgaagatct cttatccgaa aagaattcac tggaaaaaga gctagacgaa      840

aagatggaca ggtggactta cttaaatgaa ctcaatgaaa aaattgagca agataaaagc      900

actagacatt gttattagca ctgtctagtg ctagcgccat tcgccattca ggctgmgcaa      960

ctgtgggagg cgatcggtgc gggcctyttc gctattacgc cagctgcgaa aggggatgtg     1020

ctgcaagcga ttagttgggt aacsccaggc tttcccagtc mcgacgtgta aacgacgcag     1080

tgcagctgca tgtctgcagc tcgagatctc atgacgckac gtcgactcta rgatccctgt     1140

wcgagctcga ttcgaatcat gcawtggatc msggccgatc tgmccctakg cataacatgc     1200

aattcmcttt ctcattagaa aygt                                            1224


<210>  23
<211>  1120
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  23
grrammttkw aawttaaraa attkcactta tracatacta aataatgaca gartaggkat       60

tattggacct aatggaagtg gtaaatccac tcttatgaat attttarttc aaaaaattct      120

gccsgacagc ggcactmtwk atataggtga aacagtaaag ataggatact attctcaggg      180

aatttctgat atggatatga acgaaagggk aattgaatac atacktggga catcrraata      240

tgcgtcaact tcrtcgggtg aaaaaakaag tgcttctgct gtacttgaaa attttttatt      300

tgaaccttca gttcaatgga ctycccttgg aaaamtktca ggaggagaac raaraagatt      360

atatcttcta aaaatactga tgaattaccc taatgtactt ttgctggacg aaccaacaaa      420

tgatctggat atagaaacac ttacmatcct agaagattat attwacgatt ttgaaggagc      480

tgttatagct gtrtctcatg acaggtactt ccwtgacaar acagttgatr aaatattttc      540

ttttgaagga aacggtaaaa tcactcaata tacasgaaac tattcctatt ttcacrraac      600

tgcawaaata tcagaggaaa aaatwagctc taagaaaaat acagctaaaa caactatrag      660

ttgcaaaaag aawagcctct aaatttacct atgcagaaca aatggagttt gacaaaatag      720

atgaggtaay tgaacatcta gaaaatctat atctgaaaga aaatagaatg gaagctgctt      780

catctgatta tgcacttctt garatctctt atccgaaaas rattcmctgg aaaagagcta      840

gacgaaagat ggwcagkkga cttactwaat gactcatgaa aatgakcara tawagcmcta      900

gamttgttat tagcactgtc trkgstagcg ccatcgcatt cagctgmgca actgtgggac      960

ggcgatcgtk cggctcytcg ctattacgcc agctggcaag ggaktgcctg caggcatagt     1020

gttacscwgc ttccagtccm srtkaamgac gcakgccagc tgcatgtygc agcctggatc     1080

catggacgka gctcaacyta agaatccggt ccgrcactgt                           1120


<210>  24
<211>  1210
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  24
traaaawttk tcactkkata acatactaaa taatgmcaga rtaggtatta ttggacctaa       60

tggaagtggt aawtccactc ttatgaatat tttarttcaa aaaattctgc cggacagcgg      120

cacymtagat ataggtgaaa cagyaaagat aggatactat tctcagggaa tttctgatat      180

ggatatgaac saaagggkaa ttgaatacat mcktgggaca tcrraakatg cstcaacttc      240

rtcgggtgaa aaaakaaktg cttctgctgt acttgaaaat tttttatttg aaccttcagt      300

tcaatggact ccccttggaa aactktcarg aggagaacga araarattat atcttctaaa      360

aatactgatg aattacccta atgtactttt gctggacgaa ccaacaaatg atctggatat      420

agaaacactt acaatcctag aagattatat taacgatttt gaaggagctg ttatagctgt      480

rtctcatgac aggtacttcc ttgacaarac agttgataaa atattttctt ttgaaggaaa      540

cggtaaratc actcaatata caggaaacta ttcctatttt cacaaaactg cawaaatatc      600

agaggaaaaa ataagctcta agaaaaatac agctaaaaac aackatragt tgcaaaaaga      660

aaagcctcta aratttacct atgcagaaca aatggagttt gacaaaatag atgaggtaat      720

tgaacatcta gaaaaatcta tatctgaaaa gaaaatagaa atggaagctg cttcatctga      780

ttatgcactt cttgaagatc tcttatccga aaagaattca ctggaaaaag agctagacka      840

aaagatggac aggtggactt acttaaatga actcaatgaa raaattgagc aagataaaag      900

cactagacat tgttattagc actgtctagt gctagcgcca ttcgccattm agctgmgcac      960

tgtgggaagg cgatcggtgc gggctctcgc tatacgcagc tggcgaaggg gatgtgctgc     1020

agcgatagtg gtacgcakgt ttccagtcac gacgwgaaaa cgacgtcagt gcwagctgca     1080

kkctgcagct cgratctcat ggacgctkac gtcgayctra cgatcccwgt wckrctcgat     1140

cgtatcctgt cawtgatacc gggcgcatac atgccctagt cagttaacat gcaagtckac     1200

cttctcagtg                                                            1210


<210>  25
<211>  1206
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  25
aamywaatwr ggwggggttt sycaakktta tatcsttcat kttctaccca ytccctcrac       60

twagcwwatw cararatgtt arttckraat atgagcccct taaaacrgac ttcscacaaa      120

ggactccwgg yaakywyctk gwtcccttta cawtctcctt waycgraakg gmaakktctg      180

yatcyttgcc agaaggattg wwttcagcgc tgygwtaaat agttattggc ttrccwmkaa      240

agtcaattac aaaaatatat ataaagaaag caaagctacw tatwtyamwk srttwaaggt      300

aaarmtaaaa atattataaa awtgaamttw ttttttctcw takctaaagt tacwtaatac      360

gaggaggatt wataatgaaa aaagtaatwg gaattataar yattgwmcww tttgtactmk      420

tagcacttca atcytgtgck gcakgakwwg gaartgymty awgwaatrac mwwsawkswa      480

gwgratctgc wggatwattt ttatytgkat gkatgctkat tgctggaata atagcaatra      540

waycawamyw wwktamaggt atswctataa cagctatagk atwtwatttg ttakcttttg      600

ttgyagggat wgctaaygwt gggcattttw cagattwgcm wmtttgrtca aycwttrmct      660

tgatatttgc tggactatwg atattkmatt trctkaamaw yaagcaatta tatwatakca      720

gwggraaaaa gtagaatcat atrttgtaat tatttttaat tatgttgrca amtytgawmy      780

trwcacwgaw acmcctmtaa atgttttram tacatrtgtt waaktwtkgt gacakatwct      840

aatagtakra agwagaarwt ygctatgtwa tratgacata gwggtgaatg taatgcggms      900

gctgwrtcca tatsaccatg atrcgamtyc gagctcggta csssggrgat sctctrgast      960

ckacgtcack cgtccatgka gatcwcgagg ctgcmgwcwg cagmtrcwct ggtmcgtcga     1020

tktaywcgtc gtgactrsga aaaccatgac gtmctrctay ggcatgcwgc artcyccgwt     1080

tckcagstag gtawagcgac akgcgatcsm aygcccttgc cacrttgcca tctgaatgyg     1140

akgcctgaca kmcgkccmta cagctagcat gactgaattt catgackcyt agaagacmct     1200

gtgaca                                                                1206


<210>  26
<211>  1232
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  26
accymwaaat aattgmcaga ataggtatta ttggacctaa tggaagtggt aaatccactc       60

ttatgaatat tttarttcaa aaaattctgc cggacagcgg cactmtagat ataggtgaaa      120

cagtaaagat aggatactat tctcagggaa tttctgatat ggatatgaac gaaagggtaa      180

ttgaatacat acktgggaca tcrraakatg cstcaacttc atcgggtgaa aaaataaktg      240

cttctgctgt acttgaaaat tttttatttg aaccttcagt tcaatggact ccccttggaa      300

aactktcarg aggagaacra araarattat atcttctaaa aatactgatg aattacccta      360

atgtactttt gctggacgaa ccaacaaatg atctggatat agaaacactt acaatcctag      420

aagattatat taacgatttt gaaggagctg ttatagctgt atctcatgac aggtacttcc      480

ttgacaaaac asttgataaa atattttctt ttgaaggaaa cggtwaaatc actcaatata      540

caggaaacta ttcctatttt cacaaaactg caaaaatatc agaggaaaaa ataagctcta      600

agaaaaatac agctaaaaac aactatragt tgcwaaaaga aaagcctcta aaatttacct      660

atgcagaaca aatggagttt gacaaartag atgaggtaat tgaacatcta gaaaaatcta      720

tatctgaaaa gaaaatagaa atggaagctg cttcatctga ttatgcactt cttgaagatc      780

tcttatccga aaasaattca ctggaaaaag agctagacga aaagatggac aggtggactt      840

acttawatga actcatgaaa aaatwgagca gataaaagca ctagacattg ttattagcac      900

tgtstagtgc tagcgccatt cgmcattcag ctgmgcacwg ytgggaaggg cgatmgygck      960

ggcctcttcg ctwytacgyc akctggcraa ggggatgtgc wgcaagmcga tagttgggta     1020

acgcaggwtw tcccagtcac kacgtagtam aygacgtcmg trctagctgc atgtctgcag     1080

cytsrgatct catgtacgct gacgtcgacy trrmggatcc cwggtacyga gctcgattcg     1140

tatcatgayr atggatgmgc ggcgcataac tcccttatgc mttaacrttc aattsacgtc     1200

ttcwtataga tckctcatag ttgarcmccg gg                                   1232


<210>  27
<211>  37
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  27
ccgaattcgt cgacaacaga gtttgatcct ggctcag                                37


<210>  28
<211>  37
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  28
cccgggatcc aagcttacgg ctaccttgtt acgactt                                37


<210>  29
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  29
ttgctgtagt cactgaactg gaaaa                                             25


<210>  30
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  30
aatcaggaca cctaaatcca accac                                             25


<210>  31
<211>  1940
<212>  DNA
<213>  artificial sequence

<220>
<223>  novel methyltransferase gene fused with an inducible lac Promoter

<400>  31
gcggccgcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca       60

ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg      120

aaacacatat gtttccgtgc aatgcctata tcgaatatgg tgataaaaat atgaacagct      180

ttatcgaaga tgtggaacag atctacaact tcattaaaaa gaacattgat gtggaagaaa      240

agatgcattt cattgaaacc tataaacaga aaagcaacat gaagaaagag attagcttta      300

gcgaagaata ctataaacag aagattatga acggcaaaaa tggcgttgtg tacaccccgc      360

cggaaatggc ggcctttatg gttaaaaatc tgatcaacgt taacgatgtt attggcaatc      420

cgtttattaa aatcattgac ccgagctgcg gtagcggcaa tctgatttgc aaatgttttc      480

tgtatctgaa tcgcatcttt attaagaaca ttgaggtgat taacagcaaa aataacctga      540

atctgaaact ggaagacatc agctaccaca tcgttcgcaa caatctgttt ggcttcgata      600

ttgacgaaac cgcgatcaaa gtgctgaaaa ttgatctgtt tctgatcagc aaccaattta      660

gcgagaaaaa tttccaggtt aaagactttc tggtggaaaa tattgatcgc aaatatgacg      720

tgttcattgg taatccgccg tatatcggtc acaaaagcgt ggacagcagc tacagctacg      780

tgctgcgcaa aatctacggc agcatctacc gcgacaaagg cgatatcagc tattgtttct      840

ttcagaagag cctgaaatgt ctgaaggaag gtggcaaact ggtgtttgtg accagccgct      900

acttctgcga gagctgcagc ggtaaagaac tgcgtaaatt cctgatcgaa aacacgagca      960

tttacaagat cattgatttt tacggcatcc gcccgttcaa acgcgtgggt atcgatccga     1020

tgattatttt tctggttcgt acgaagaact ggaacaataa cattgaaatt attcgcccga     1080

acaagattga aaagaacgaa aagaacaaat tcctggatag cctgttcctg gacaaaagcg     1140

aaaagtgtaa aaagtttagc attagccaga aaagcattaa taacgatggc tgggttttcg     1200

tggacgaagt ggagaaaaac attatcgaca aaatcaaaga gaaaagcaag ttcattctga     1260

aagatatttg ccatagctgt caaggcatta tcaccggttg tgatcgcgcc tttattgtgg     1320

accgtgatat catcaatagc cgtaagatcg aactgcgtct gattaaaccg tggattaaaa     1380

gcagccatat ccgtaagaat gaagttatta agggcgaaaa attcatcatc tatagcaacc     1440

tgattgagaa tgaaaccgag tgtccgaatg cgattaaata tatcgaacag tacaagaaac     1500

gtctgatgga gcgccgcgaa tgcaaaaagg gcacgcgtaa gtggtatgaa ctgcaatggg     1560

gccgtaaacc ggaaatcttc gaagaaaaga aaattgtttt cccgtataaa agctgtgaca     1620

atcgttttgc actggataag ggtagctatt ttagcgcaga catttatagc ctggttctga     1680

agaaaaatgt gccgttcacc tatgagatcc tgctgaatat cctgaatagc ccgctgtacg     1740

agttttactt taagaccttc gcgaaaaagc tgggcgagaa tctgtacgag tactatccga     1800

acaacctgat gaagctgtgc atcccgagca tcgatttcgg cggtgagaac aatattgaga     1860

aaaagctgta tgatttcttt ggtctgacgg ataaagaaat tgagattgtg gagaagatca     1920

aagataactg ctaagaattc                                                 1940


<210>  32
<211>  601
<212>  PRT
<213>  Clostridium autoethanogenum

<400>  32

Met Phe Pro Cys Asn Ala Tyr Ile Glu Tyr Gly Asp Lys Asn Met Asn 
1               5                   10                  15      


Ser Phe Ile Glu Asp Val Glu Gln Ile Tyr Asn Phe Ile Lys Lys Asn 
            20                  25                  30          


Ile Asp Val Glu Glu Lys Met His Phe Ile Glu Thr Tyr Lys Gln Lys 
        35                  40                  45              


Ser Asn Met Lys Lys Glu Ile Ser Phe Ser Glu Glu Tyr Tyr Lys Gln 
    50                  55                  60                  


Lys Ile Met Asn Gly Lys Asn Gly Val Val Tyr Thr Pro Pro Glu Met 
65                  70                  75                  80  


Ala Ala Phe Met Val Lys Asn Leu Ile Asn Val Asn Asp Val Ile Gly 
                85                  90                  95      


Asn Pro Phe Ile Lys Ile Ile Asp Pro Ser Cys Gly Ser Gly Asn Leu 
            100                 105                 110         


Ile Cys Lys Cys Phe Leu Tyr Leu Asn Arg Ile Phe Ile Lys Asn Ile 
        115                 120                 125             


Glu Val Ile Asn Ser Lys Asn Asn Leu Asn Leu Lys Leu Glu Asp Ile 
    130                 135                 140                 


Ser Tyr His Ile Val Arg Asn Asn Leu Phe Gly Phe Asp Ile Asp Glu 
145                 150                 155                 160 


Thr Ala Ile Lys Val Leu Lys Ile Asp Leu Phe Leu Ile Ser Asn Gln 
                165                 170                 175     


Phe Ser Glu Lys Asn Phe Gln Val Lys Asp Phe Leu Val Glu Asn Ile 
            180                 185                 190         


Asp Arg Lys Tyr Asp Val Phe Ile Gly Asn Pro Pro Tyr Ile Gly His 
        195                 200                 205             


Lys Ser Val Asp Ser Ser Tyr Ser Tyr Val Leu Arg Lys Ile Tyr Gly 
    210                 215                 220                 


Ser Ile Tyr Arg Asp Lys Gly Asp Ile Ser Tyr Cys Phe Phe Gln Lys 
225                 230                 235                 240 


Ser Leu Lys Cys Leu Lys Glu Gly Gly Lys Leu Val Phe Val Thr Ser 
                245                 250                 255     


Arg Tyr Phe Cys Glu Ser Cys Ser Gly Lys Glu Leu Arg Lys Phe Leu 
            260                 265                 270         


Ile Glu Asn Thr Ser Ile Tyr Lys Ile Ile Asp Phe Tyr Gly Ile Arg 
        275                 280                 285             


Pro Phe Lys Arg Val Gly Ile Asp Pro Met Ile Ile Phe Leu Val Arg 
    290                 295                 300                 


Thr Lys Asn Trp Asn Asn Asn Ile Glu Ile Ile Arg Pro Asn Lys Ile 
305                 310                 315                 320 


Glu Lys Asn Glu Lys Asn Lys Phe Leu Asp Ser Leu Phe Leu Asp Lys 
                325                 330                 335     


Ser Glu Lys Cys Lys Lys Phe Ser Ile Ser Gln Lys Ser Ile Asn Asn 
            340                 345                 350         


Asp Gly Trp Val Phe Val Asp Glu Val Glu Lys Asn Ile Ile Asp Lys 
        355                 360                 365             


Ile Lys Glu Lys Ser Lys Phe Ile Leu Lys Asp Ile Cys His Ser Cys 
    370                 375                 380                 


Gln Gly Ile Ile Thr Gly Cys Asp Arg Ala Phe Ile Val Asp Arg Asp 
385                 390                 395                 400 


Ile Ile Asn Ser Arg Lys Ile Glu Leu Arg Leu Ile Lys Pro Trp Ile 
                405                 410                 415     


Lys Ser Ser His Ile Arg Lys Asn Glu Val Ile Lys Gly Glu Lys Phe 
            420                 425                 430         


Ile Ile Tyr Ser Asn Leu Ile Glu Asn Glu Thr Glu Cys Pro Asn Ala 
        435                 440                 445             


Ile Lys Tyr Ile Glu Gln Tyr Lys Lys Arg Leu Met Glu Arg Arg Glu 
    450                 455                 460                 


Cys Lys Lys Gly Thr Arg Lys Trp Tyr Glu Leu Gln Trp Gly Arg Lys 
465                 470                 475                 480 


Pro Glu Ile Phe Glu Glu Lys Lys Ile Val Phe Pro Tyr Lys Ser Cys 
                485                 490                 495     


Asp Asn Arg Phe Ala Leu Asp Lys Gly Ser Tyr Phe Ser Ala Asp Ile 
            500                 505                 510         


Tyr Ser Leu Val Leu Lys Lys Asn Val Pro Phe Thr Tyr Glu Ile Leu 
        515                 520                 525             


Leu Asn Ile Leu Asn Ser Pro Leu Tyr Glu Phe Tyr Phe Lys Thr Phe 
    530                 535                 540                 


Ala Lys Lys Leu Gly Glu Asn Leu Tyr Glu Tyr Tyr Pro Asn Asn Leu 
545                 550                 555                 560 


Met Lys Leu Cys Ile Pro Ser Ile Asp Phe Gly Gly Glu Asn Asn Ile 
                565                 570                 575     


Glu Lys Lys Leu Tyr Asp Phe Phe Gly Leu Thr Asp Lys Glu Ile Glu 
            580                 585                 590         


Ile Val Glu Lys Ile Lys Asp Asn Cys 
        595                 600     


<210>  33
<211>  2781
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic plasmid

<400>  33
tttgccacct gacgtctaag aaaaggaata ttcagcaatt tgcccgtgcc gaagaaaggc       60

ccacccgtga aggtgagcca gtgagttgat tgctacgtaa ttagttagtt agcccttagt      120

gactcgtaat acgactcact atagggctcg agtctagaga attcgatatc acccgggaac      180

tagtctgcag ccctttagtg agggttaatt ggagtcacta agggttagtt agttagatta      240

gcagaaagtc aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca      300

ttggggctca ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg      360

atttctgcta gagatggaat agactggatg gaggcggata aagttgcagg accacttctg      420

cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg      480

tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc      540

tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt      600

gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt      660

gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc      720

atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cttaataaga      780

tgatcttctt gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc      840

gccttgcagg gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac      900

tggcttggag gagcgcagtc accaaaactt gtcctttcag tttagcctta accggcgcat      960

gacttcaaga ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc     1020

atgtctttcc gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg     1080

aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt     1140

caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg     1200

caggaacagg agagcgcacg agggagccgc caggggaaac gcctggtatc tttatagtcc     1260

tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg     1320

gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg     1380

gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg     1440

accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc     1500

tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg acaccctcat     1560

cagtgccaac atagtaagcc agtatacact ccgctagcgc tgaggtctgc ctcgtgaaga     1620

aggtgttgct gactcatacc aggcctgaat cgccccatca tccagccaga aagtgaggga     1680

gccacggttg atgagagctt tgttgtaggt ggaccagttg gtgattttga acttttgctt     1740

tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca actcagcaaa     1800

agttcgattt attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca     1860

agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag     1920

gggtgtttac tagaggttga tcgggcacgt aagaggttcc aactttcacc ataatgaaat     1980

aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa     2040

aatggagaaa aaaatcacgg gatataccac cgttgatata tcccaatggc atcgtaaaga     2100

acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga     2160

tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat     2220

tcacattctt gcccgcctga tgaacgctca cccggagttt cgtatggcca tgaaagacgg     2280

tgagctggtg atctgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga     2340

aacgttttcg tccctctgga gtgaatacca cgacgatttc cggcagtttc tccacatata     2400

ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga     2460

gaatatgttt tttgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt     2520

ggccaatatg gacaacttct tcgcccccgt tttcacgatg ggcaaatatt atacgcaagg     2580

cgacaaggtg ctgatgccgc tggcgatcca ggttcatcat gccgtttgtg atggcttcca     2640

tgtcggccgc atgcttaatg aattacaaca gtactgtgat gagtggcagg gcggggcgta     2700

ataatactag ctccggcaaa aaaacgggca aggtgtcacc accctgccct ttttctttaa     2760

aaccgaaaag attacttcgc g                                               2781


<210>  34
<211>  351
<212>  PRT
<213>  Clostridium autoethanogenum

<400>  34

Met Lys Gly Phe Ala Met Leu Gly Ile Asn Lys Leu Gly Trp Ile Glu 
1               5                   10                  15      


Lys Lys Asn Pro Val Pro Gly Pro Tyr Asp Ala Ile Val His Pro Leu 
            20                  25                  30          


Ala Val Ser Pro Cys Thr Ser Asp Ile His Thr Val Phe Glu Gly Ala 
        35                  40                  45              


Leu Gly Asn Arg Glu Asn Met Ile Leu Gly His Glu Ala Val Gly Glu 
    50                  55                  60                  


Ile Ala Glu Val Gly Ser Glu Val Lys Asp Phe Lys Val Gly Asp Arg 
65                  70                  75                  80  


Val Ile Val Pro Cys Thr Thr Pro Asp Trp Arg Ser Leu Glu Val Gln 
                85                  90                  95      


Ala Gly Phe Gln Gln His Ser Asn Gly Met Leu Ala Gly Trp Lys Phe 
            100                 105                 110         


Ser Asn Phe Lys Asp Gly Val Phe Ala Asp Tyr Phe His Val Asn Asp 
        115                 120                 125             


Ala Asp Met Asn Leu Ala Ile Leu Pro Asp Glu Ile Pro Leu Glu Ser 
    130                 135                 140                 


Ala Val Met Met Thr Asp Met Met Thr Thr Gly Phe His Gly Ala Glu 
145                 150                 155                 160 


Leu Ala Asp Ile Lys Met Gly Ser Ser Val Val Val Ile Gly Ile Gly 
                165                 170                 175     


Ala Val Gly Leu Met Gly Ile Ala Gly Ser Lys Leu Arg Gly Ala Gly 
            180                 185                 190         


Arg Ile Ile Gly Val Gly Ser Arg Pro Val Cys Val Glu Thr Ala Lys 
        195                 200                 205             


Phe Tyr Gly Ala Thr Asp Ile Val Asn Tyr Lys Asn Gly Asp Ile Val 
    210                 215                 220                 


Glu Gln Ile Met Asp Leu Thr His Gly Lys Gly Val Asp Arg Val Ile 
225                 230                 235                 240 


Met Ala Gly Gly Gly Ala Glu Thr Leu Ala Gln Ala Val Thr Met Val 
                245                 250                 255     


Lys Pro Gly Gly Val Ile Ser Asn Ile Asn Tyr His Gly Ser Gly Asp 
            260                 265                 270         


Thr Leu Pro Ile Pro Arg Val Gln Trp Gly Cys Gly Met Ala His Lys 
        275                 280                 285             


Thr Ile Arg Gly Gly Leu Cys Pro Gly Gly Arg Leu Arg Met Glu Met 
    290                 295                 300                 


Leu Arg Asp Leu Val Leu Tyr Lys Arg Val Asp Leu Ser Lys Leu Val 
305                 310                 315                 320 


Thr His Val Phe Asp Gly Ala Glu Asn Ile Glu Lys Ala Leu Leu Leu 
                325                 330                 335     


Met Lys Asn Lys Pro Lys Asp Leu Ile Lys Ser Val Val Thr Phe 
            340                 345                 350     


<210>  35
<211>  1056
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  35
atgaaaggtt ttgcaatgtt aggtattaac aaattaggat ggattgaaaa gaaaaaccca       60

gtgccaggtc cttatgatgc gattgtacat cctctagctg tatccccatg tacatcagat      120

atacatacgg tttttgaagg agcacttggt aatagggaaa atatgatttt aggccatgaa      180

gctgtaggtg aaatagccga agttggcagc gaagttaaag attttaaagt tggcgataga      240

gttatcgtac catgcacaac acctgactgg agatctttag aagtccaagc tggttttcag      300

cagcattcaa acggtatgct tgcaggatgg aagttttcca attttaaaga cggtgtattt      360

gcagattact ttcatgtaaa cgatgcagat atgaatcttg ccatactccc agatgaaata      420

cctttagaaa gtgcagttat gatgacagac atgatgacta ctggttttca tggagcagaa      480

cttgcagaca taaaaatggg ctccagcgtt gtagtaattg gtataggagc tgttggatta      540

atgggaatag ccggttccaa acttcgagga gcaggcagaa ttatcggtgt tggaagcaga      600

cctgtttgtg ttgaaacagc taaattttat ggagcaactg atattgtaaa ttataaaaat      660

ggtgatatag ttgaacaaat catggactta actcatggta aaggtgtaga ccgtgtaatc      720

atggcaggcg gtggtgctga aacactagca caagcagtaa ctatggttaa acctggcggc      780

gtaatttcta acatcaacta ccatggaagc ggtgatactt taccaatacc tcgtgttcaa      840

tggggctgcg gcatggctca caaaactata agaggaggat tatgccccgg cggacgtctt      900

agaatggaaa tgctaagaga tcttgttcta tataaacgtg ttgatttgag taaacttgtt      960

actcatgtat ttgatggtgc agaaaatatt gaaaaggccc ttttgcttat gaaaaataag     1020

ccaaaagatt taattaaatc agtagttaca ttctaa                               1056


<210>  36
<211>  1056
<212>  DNA
<213>  Clostridium ljungdahlii

<400>  36
atgaaaggtt ttgcaatgtt aggtattaac aaattaggat ggattgaaaa gaaaaaccca       60

gtgccaggtc cttatgatgc gattgtacat cctctagctg tatccccatg tacatcagat      120

atacatacgg tttttgaagg agcacttggt aatagggaaa atatgatttt aggccatgaa      180

gctgtaggtg aaatagccga agttggcagc gaagttaaag attttaaagt tggcgataga      240

gttatcgtac catgcacaac acctgactgg agatctttag aagtccaagc tggttttcag      300

cagcattcaa acggtatgct tgcaggatgg aagttttcca attttaaaga tggtgtattt      360

gcagattact ttcatgtaaa cgatgcagat atgaatcttg ccatactccc agatgaaata      420

cctttagaaa gtgcagttat gatgacagac atgatgacta ctggttttca tggagcagaa      480

cttgcagaca taaaaatggg ctccagcgtt gtagtaattg gtataggagc tgttggatta      540

atgggaatag ccggttccaa acttcgagga gcaggcagaa ttatcggtgt tggaagcaga      600

cctgtttgtg ttgaaacagc taaattttat ggagcaactg atattgtaaa ttataaaaat      660

ggtgatatag ttgaacaaat catggactta actcatggta aaggtgtaga ccgtgtaatc      720

atggcaggcg gtggtgctga aacactagca caagcagtaa ctatggttaa acctggcggc      780

gtaatttcta acatcaacta ccatggaagc ggtgatactt taccaatacc tcgtgttcaa      840

tggggctgcg gcatggctca caaaactata agaggaggat tatgccccgg cggacgtctt      900

agaatggaaa tgctaagaga tcttgttcta tataaacgtg ttgatttgag taaacttgtt      960

actcatgtat ttgatggtgc agaaaatatt gaaaaggccc ttttgcttat gaaaaataag     1020

ccaaaagatt taattaaatc agtagttaca ttctaa                               1056


<210>  37
<211>  1056
<212>  DNA
<213>  Clostridium ragsdalei

<400>  37
atgaaaggtt ttgcaatgtt aggtattaac aagttaggat ggattgaaaa gaaaaaccca       60

gtaccaggtc cttatgatgc gattgtacat cctctagctg tatccccatg tacatcagat      120

atacatacgg tttttgaagg agcacttggt aatagggaaa atatgatttt aggtcacgaa      180

gctgtaggtg aaatagctga agttggcagt gaagttaaag attttaaagt tggcgataga      240

gttatcgtac catgcacaac acctgactgg agatccttag aagtccaagc tggttttcaa      300

cagcattcaa acggtatgct tgcaggatgg aagttttcca attttaaaga cggtgtattt      360

gcagattact ttcatgtaaa cgatgcagat atgaatcttg caatacttcc agatgaaata      420

cctttagaaa gtgcagttat gatgacagac atgatgacta ctggttttca tggggcagaa      480

cttgctgaca taaaaatggg ttccagtgtt gtcgtaattg gtataggagc tgttggatta      540

atgggaatag ccggttccaa acttcgagga gcaggtagaa ttatcggtgt tggaagcaga      600

cccgtttgtg ttgaaacagc taaattttat ggagcaactg atattgtaaa ttataaaaat      660

ggtgatatag ttgaacaaat aatggactta actcatggta aaggtgtaga ccgtgtaatc      720

atggcaggcg gtggtgctga aacactagca caagcagtaa ctatggttaa acctggcggc      780

gtaatttcta acatcaacta ccatggaagc ggtgatactt tgccaatacc tcgtgttcaa      840

tggggctgcg gcatggctca caaaactata agaggagggt tatgtcccgg cggacgtctt      900

agaatggaaa tgctaagaga ccttgttcta tataaacgtg ttgatttgag caaacttgtt      960

actcatgtat ttgatggtgc agaaaatatt gaaaaggccc ttttgcttat gaaaaataag     1020

ccaaaagatt taattaaatc agtagttaca ttctaa                               1056


<210>  38
<211>  1230
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  38
atgaacaatt taaaggaaga agcattaaag tttcataaag aacatgaagg taaaatagca       60

cttaaaagta aagtatctgt taaaactaga gaggacctag gcttagcata tactccaggt      120

gttgctgaac catgtcttga aatcaacagg gactataata cgttatacga ttatacttct      180

aagggaaatt atgtagcagt agtaactaac ggcagtgcag ttttgggact tggaaatata      240

ggtgctgcag ctggcttacc tgtaatggaa ggtaaatcta ttctatttaa gacttttgca      300

ggagtagacg cttttcctat ttgtgttgac agcaaagatc ctgacaagat tgtagaaaca      360

gtaaaattaa tagaatccac atttggagga ataaacctag aagatataaa agcacctgag      420

tgctttgaaa tagaagataa attaaaaaag gtctgcaata taccagtttt tcatgacgac      480

cagcacggaa cagcagtagt aactttagct gctatgataa atgcacttaa aatagtaaac      540

aaaaaatttg aagacttaaa agtaataata aatggtgcag gagctgcagg tacagcaatt      600

gcaaaactgc ttgtaagtag aggagttaaa aacattattg tatgcgatag aaaaggtgct      660

atatcaaaag atagagaaaa tttaagtgct gcaaaaaaag acctagcaga agttacaaat      720

cctagtatga taaaaggtgc acttaaagat gtactaaaag aagctgatgt attcataggt      780

gtatctgctc ctggagtaat tactcctgaa atgataaaaa caatggctaa agatcccctc      840

atttttgcta tggccaatcc taagcctgaa atctaccctg atgaagcaaa agctgcaggt      900

gccagagtag ttggtacggg aagatcagat ttcccaaatc aaataaataa tgttcttgca      960

tttcctggaa tatttagagg agcacttgat gtaagggcat caaaaataaa tgaagaaatg     1020

aaaatagctg ctgcatgtgc tatagcagac ataataactg aaaaagaact taatgaagat     1080

tatgttatac cagatgcttt tgactcaaga atagcaccaa aggtagctta ttatgtagca     1140

aaggctgcca tagaaagtgg agttgcaaga agaactgaca tcactcctga aatggtagaa     1200

gaacatacta aaaagcttgt acaagcataa                                      1230


<210>  39
<211>  409
<212>  PRT
<213>  Clostridium autoethanogenum

<400>  39

Met Asn Asn Leu Lys Glu Glu Ala Leu Lys Phe His Lys Glu His Glu 
1               5                   10                  15      


Gly Lys Ile Ala Leu Lys Ser Lys Val Ser Val Lys Thr Arg Glu Asp 
            20                  25                  30          


Leu Gly Leu Ala Tyr Thr Pro Gly Val Ala Glu Pro Cys Leu Glu Ile 
        35                  40                  45              


Asn Arg Asp Tyr Asn Thr Leu Tyr Asp Tyr Thr Ser Lys Gly Asn Tyr 
    50                  55                  60                  


Val Ala Val Val Thr Asn Gly Ser Ala Val Leu Gly Leu Gly Asn Ile 
65                  70                  75                  80  


Gly Ala Ala Ala Gly Leu Pro Val Met Glu Gly Lys Ser Ile Leu Phe 
                85                  90                  95      


Lys Thr Phe Ala Gly Val Asp Ala Phe Pro Ile Cys Val Asp Ser Lys 
            100                 105                 110         


Asp Pro Asp Lys Ile Val Glu Thr Val Lys Leu Ile Glu Ser Thr Phe 
        115                 120                 125             


Gly Gly Ile Asn Leu Glu Asp Ile Lys Ala Pro Glu Cys Phe Glu Ile 
    130                 135                 140                 


Glu Asp Lys Leu Lys Lys Val Cys Asn Ile Pro Val Phe His Asp Asp 
145                 150                 155                 160 


Gln His Gly Thr Ala Val Val Thr Leu Ala Ala Met Ile Asn Ala Leu 
                165                 170                 175     


Lys Ile Val Asn Lys Lys Phe Glu Asp Leu Lys Val Ile Ile Asn Gly 
            180                 185                 190         


Ala Gly Ala Ala Gly Thr Ala Ile Ala Lys Leu Leu Val Ser Arg Gly 
        195                 200                 205             


Val Lys Asn Ile Ile Val Cys Asp Arg Lys Gly Ala Ile Ser Lys Asp 
    210                 215                 220                 


Arg Glu Asn Leu Ser Ala Ala Lys Lys Asp Leu Ala Glu Val Thr Asn 
225                 230                 235                 240 


Pro Ser Met Ile Lys Gly Ala Leu Lys Asp Val Leu Lys Glu Ala Asp 
                245                 250                 255     


Val Phe Ile Gly Val Ser Ala Pro Gly Val Ile Thr Pro Glu Met Ile 
            260                 265                 270         


Lys Thr Met Ala Lys Asp Pro Leu Ile Phe Ala Met Ala Asn Pro Lys 
        275                 280                 285             


Pro Glu Ile Tyr Pro Asp Glu Ala Lys Ala Ala Gly Ala Arg Val Val 
    290                 295                 300                 


Gly Thr Gly Arg Ser Asp Phe Pro Asn Gln Ile Asn Asn Val Leu Ala 
305                 310                 315                 320 


Phe Pro Gly Ile Phe Arg Gly Ala Leu Asp Val Arg Ala Ser Lys Ile 
                325                 330                 335     


Asn Glu Glu Met Lys Ile Ala Ala Ala Cys Ala Ile Ala Asp Ile Ile 
            340                 345                 350         


Thr Glu Lys Glu Leu Asn Glu Asp Tyr Val Ile Pro Asp Ala Phe Asp 
        355                 360                 365             


Ser Arg Ile Ala Pro Lys Val Ala Tyr Tyr Val Ala Lys Ala Ala Ile 
    370                 375                 380                 


Glu Ser Gly Val Ala Arg Arg Thr Asp Ile Thr Pro Glu Met Val Glu 
385                 390                 395                 400 


Glu His Thr Lys Lys Leu Val Gln Ala 
                405                 


<210>  40
<211>  1230
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  40
atgaatctaa gagaaactgc attaaaattt cacaaagaca acgaaggtaa aattgcacta       60

aaatgcaagg tgccggttaa aaacaaagaa gacctaacgt tagcatatac acctggagtt      120

gcagaacctt gcttagaaat taataaaaat ccagaatgca tttatgacta tacatcaaaa      180

gggaattggg tagctgttgt aacaaatggt acagctgttc ttggccttgg aaatataggt      240

gcaggtgcag gacttcctgt tatggaggga aaatccgttt tatttaaaac ttttgctgga      300

gtagatgcat ttccaatatg tctggaaagc aaggatatca atgaaattgt agcagctgta      360

aaacttatgg aaccaacttt tggaggaata aatctagagg acatcaaagc tccagaatgc      420

tttgaaattg aatcaaagct taaagaagtt tgtaacatcc ctgtatttca tgacgatcaa      480

catggaacgg cagttgtttc atcagcctgt cttataaatg cattaaagat agtaaataaa      540

aaatttgaag acttaaaaat tgttgtaaat ggagcaggag cagcaggaac tgccattaca      600

aaacttttaa taaagatggg aacaaaaaat gtaatacttt gcgacactaa aggtgctata      660

tacaagagaa gaccaattgg aatgaataag tttaaggatg aaatggcaga aataacaaat      720

cctaatcttc agaaaggaac tcttgctgat gttttaaaag gtgcagatgt atttttagga      780

gtatctgcag ctaattgtgt aactgaagaa atggtaaagt ccatgaataa agattcaata      840

attatggcaa tggcaaatcc aaatccagaa atacttcctg atttagctat aaaagctgga      900

gctaaagtgg tatgtacagg aaggtcggat tttccaaatc aggttaacaa tgtacttgct      960

tttccaggaa tatttagggg agctttagat gtaagggcaa gtgaaataaa tgatgagatg     1020

aaaatagctg cagcatatgc aatagcagaa cttgtaagtg aagaagaatt gaaaccagac     1080

tatataatac ctaatgcttt tgacttgaga atagccccaa aagtagccgc atacgtagca     1140

aaagctgcta ttgatacagg tgttgcgagg aaaaaggatg tcactccaga gatggttgaa     1200

aaacatacta agactttgct tggaatctaa                                      1230


<210>  41
<211>  409
<212>  PRT
<213>  Clostridium autoethanogenum

<400>  41

Met Asn Leu Arg Glu Thr Ala Leu Lys Phe His Lys Asp Asn Glu Gly 
1               5                   10                  15      


Lys Ile Ala Leu Lys Cys Lys Val Pro Val Lys Asn Lys Glu Asp Leu 
            20                  25                  30          


Thr Leu Ala Tyr Thr Pro Gly Val Ala Glu Pro Cys Leu Glu Ile Asn 
        35                  40                  45              


Lys Asn Pro Glu Cys Ile Tyr Asp Tyr Thr Ser Lys Gly Asn Trp Val 
    50                  55                  60                  


Ala Val Val Thr Asn Gly Thr Ala Val Leu Gly Leu Gly Asn Ile Gly 
65                  70                  75                  80  


Ala Gly Ala Gly Leu Pro Val Met Glu Gly Lys Ser Val Leu Phe Lys 
                85                  90                  95      


Thr Phe Ala Gly Val Asp Ala Phe Pro Ile Cys Leu Glu Ser Lys Asp 
            100                 105                 110         


Ile Asn Glu Ile Val Ala Ala Val Lys Leu Met Glu Pro Thr Phe Gly 
        115                 120                 125             


Gly Ile Asn Leu Glu Asp Ile Lys Ala Pro Glu Cys Phe Glu Ile Glu 
    130                 135                 140                 


Ser Lys Leu Lys Glu Val Cys Asn Ile Pro Val Phe His Asp Asp Gln 
145                 150                 155                 160 


His Gly Thr Ala Val Val Ser Ser Ala Cys Leu Ile Asn Ala Leu Lys 
                165                 170                 175     


Ile Val Asn Lys Lys Phe Glu Asp Leu Lys Ile Val Val Asn Gly Ala 
            180                 185                 190         


Gly Ala Ala Gly Thr Ala Ile Thr Lys Leu Leu Ile Lys Met Gly Thr 
        195                 200                 205             


Lys Asn Val Ile Leu Cys Asp Thr Lys Gly Ala Ile Tyr Lys Arg Arg 
    210                 215                 220                 


Pro Ile Gly Met Asn Lys Phe Lys Asp Glu Met Ala Glu Ile Thr Asn 
225                 230                 235                 240 


Pro Asn Leu Gln Lys Gly Thr Leu Ala Asp Val Leu Lys Gly Ala Asp 
                245                 250                 255     


Val Phe Leu Gly Val Ser Ala Ala Asn Cys Val Thr Glu Glu Met Val 
            260                 265                 270         


Lys Ser Met Asn Lys Asp Ser Ile Ile Met Ala Met Ala Asn Pro Asn 
        275                 280                 285             


Pro Glu Ile Leu Pro Asp Leu Ala Ile Lys Ala Gly Ala Lys Val Val 
    290                 295                 300                 


Cys Thr Gly Arg Ser Asp Phe Pro Asn Gln Val Asn Asn Val Leu Ala 
305                 310                 315                 320 


Phe Pro Gly Ile Phe Arg Gly Ala Leu Asp Val Arg Ala Ser Glu Ile 
                325                 330                 335     


Asn Asp Glu Met Lys Ile Ala Ala Ala Tyr Ala Ile Ala Glu Leu Val 
            340                 345                 350         


Ser Glu Glu Glu Leu Lys Pro Asp Tyr Ile Ile Pro Asn Ala Phe Asp 
        355                 360                 365             


Leu Arg Ile Ala Pro Lys Val Ala Ala Tyr Val Ala Lys Ala Ala Ile 
    370                 375                 380                 


Asp Thr Gly Val Ala Arg Lys Lys Asp Val Thr Pro Glu Met Val Glu 
385                 390                 395                 400 


Lys His Thr Lys Thr Leu Leu Gly Ile 
                405                 


<210>  42
<211>  1098
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  42
atgtcataca ccaaagttaa atatgaagat ataaaaaagc tgtgtaattt ggtctttgag       60

aaatttggat tcaaccggga agatagtgaa accataacta gcgttttgct tttatcagat      120

ctatatggaa ttgaatccca tggtattcaa aggctggtaa agtactacag tgaaataaaa      180

agtggtctta taaatatcaa ttctaaaata aaaatagtaa aggaaacacc tgtatctgca      240

acaatagatg gcatgggcgg tatgggacag ctaattggta aaaaagctat gaatctggca      300

attaaaaaag ctaaaacttc aggaatgagt atggtagtgg ttagaaattc aaatcactat      360

ggtattgcag gctactatgc caaaatggct gaggaggaag gacttcttgg aatttcaatg      420

accaactctc cagctgtaat ggtaccaacc tttggaaaag atgctatgct tggcacaaat      480

cctattgcca tatcttttcc agctaaaccc tacccatttt taatggatat ggctactagc      540

gtagttacta ggggaaaaat tgaagtttat aacaaaaggc atgaacctct tccccttggt      600

ctagctttaa atagtgatgg tgaagatact acagatccct tagatgtact tcttaatgta      660

cgaaaaaatt ctggaggagg actgcttcct cttggaggat caaaagaatc aactggagga      720

cataaaggtt atggatttgc acttgcagtt gaaatgttta cagcaatttt atctggagga      780

tttactgcaa ataaagttag cttagatagg gaaaatggat ctggaacatg tcattatttc      840

tttgcagtgg attatggtat atttggggat aaacaatcca ttgaagagaa cttttccagc      900

tacctaaatg aacttagaaa ttcaaagaaa gcaaaaggcg ccacaagaat atatactcat      960

ggtgagaaag aagtagaatc ctataaggat aaaatgaaaa atggaattcc agtaaacgac     1020

actactctta aagaaatata cgacatatgt gactacttta gcataaaagc tagtgactat     1080

gtaactaaag tagtataa                                                   1098


<210>  43
<211>  365
<212>  PRT
<213>  Clostridium autoethanogenum

<400>  43

Met Ser Tyr Thr Lys Val Lys Tyr Glu Asp Ile Lys Lys Leu Cys Asn 
1               5                   10                  15      


Leu Val Phe Glu Lys Phe Gly Phe Asn Arg Glu Asp Ser Glu Thr Ile 
            20                  25                  30          


Thr Ser Val Leu Leu Leu Ser Asp Leu Tyr Gly Ile Glu Ser His Gly 
        35                  40                  45              


Ile Gln Arg Leu Val Lys Tyr Tyr Ser Glu Ile Lys Ser Gly Leu Ile 
    50                  55                  60                  


Asn Ile Asn Ser Lys Ile Lys Ile Val Lys Glu Thr Pro Val Ser Ala 
65                  70                  75                  80  


Thr Ile Asp Gly Met Gly Gly Met Gly Gln Leu Ile Gly Lys Lys Ala 
                85                  90                  95      


Met Asn Leu Ala Ile Lys Lys Ala Lys Thr Ser Gly Met Ser Met Val 
            100                 105                 110         


Val Val Arg Asn Ser Asn His Tyr Gly Ile Ala Gly Tyr Tyr Ala Lys 
        115                 120                 125             


Met Ala Glu Glu Glu Gly Leu Leu Gly Ile Ser Met Thr Asn Ser Pro 
    130                 135                 140                 


Ala Val Met Val Pro Thr Phe Gly Lys Asp Ala Met Leu Gly Thr Asn 
145                 150                 155                 160 


Pro Ile Ala Ile Ser Phe Pro Ala Lys Pro Tyr Pro Phe Leu Met Asp 
                165                 170                 175     


Met Ala Thr Ser Val Val Thr Arg Gly Lys Ile Glu Val Tyr Asn Lys 
            180                 185                 190         


Arg His Glu Pro Leu Pro Leu Gly Leu Ala Leu Asn Ser Asp Gly Glu 
        195                 200                 205             


Asp Thr Thr Asp Pro Leu Asp Val Leu Leu Asn Val Arg Lys Asn Ser 
    210                 215                 220                 


Gly Gly Gly Leu Leu Pro Leu Gly Gly Ser Lys Glu Ser Thr Gly Gly 
225                 230                 235                 240 


His Lys Gly Tyr Gly Phe Ala Leu Ala Val Glu Met Phe Thr Ala Ile 
                245                 250                 255     


Leu Ser Gly Gly Phe Thr Ala Asn Lys Val Ser Leu Asp Arg Glu Asn 
            260                 265                 270         


Gly Ser Gly Thr Cys His Tyr Phe Phe Ala Val Asp Tyr Gly Ile Phe 
        275                 280                 285             


Gly Asp Lys Gln Ser Ile Glu Glu Asn Phe Ser Ser Tyr Leu Asn Glu 
    290                 295                 300                 


Leu Arg Asn Ser Lys Lys Ala Lys Gly Ala Thr Arg Ile Tyr Thr His 
305                 310                 315                 320 


Gly Glu Lys Glu Val Glu Ser Tyr Lys Asp Lys Met Lys Asn Gly Ile 
                325                 330                 335     


Pro Val Asn Asp Thr Thr Leu Lys Glu Ile Tyr Asp Ile Cys Asp Tyr 
            340                 345                 350         


Phe Ser Ile Lys Ala Ser Asp Tyr Val Thr Lys Val Val 
        355                 360                 365 


<210>  44
<211>  2640
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  44
atgaatggta agaagtacgt ttatcttttc aatgaaggaa atgctggcat gagaaattta       60

cttggaggca agggagctaa tcttgcagaa atgaccaatc ttggcatacc cgttcccggt      120

ggatttacta tatccacaga ggcatgtacc aaatattatg aagatggtaa atctatatcg      180

cagcaagtta tagatcaaat ttatgatgca cttaaaaatg tggaagagac aacaggaaaa      240

aaatttggaa gcatagaaaa tccattgtta gtttcagtaa gatcaggagc cagagtttct      300

atgccaggaa tgatggatac tatattaaat ttaggattaa atgatgatac tgtaatagga      360

cttaaaaagt tgacaggaaa tgaaagattt gcgtatgatt cttatagaag atttattcaa      420

atgttttcag atgtagttat gggaattgaa aagagagaat ttgaagatgt attggatgat      480

gtaaaaaatg ctaaaggagt aaaatacgat acagatttag atgagtccga tttaaagaat      540

ataatccaga gatttaagga tatttataaa aaagaagtaa aggaagactt tcctcaagat      600

cctaaagaac aattaattca gtcagttact gcagtattca gatcttggga aaatcctaga      660

gcaataattt atagaaggtt aaacgacata tcaggtgatt ggggaactgc agtaaatgtt      720

caatcaatgg tatttggaaa tatgggagaa acttcaggaa ctggagttgc atttactaga      780

aatccatcta caggagaaaa gtccatattt ggtgaatatc tcataaatgc tcaaggagag      840

gatgtagttg caggaataag aacacctcaa cctataacaa agctaaaaga agaccttcca      900

gaatgttatt ctcaatttat gagtatagca aataagcttg aaaatcatta taaagatatg      960

caggatatgg agttcactat agaacaggga aaattgtatt tccttcagac aagaaacggt     1020

aagagaacag ctcaagctgc acttagaata gcagtaaata tggtagatga aggtctcatc     1080

actaaagaag aggccatact taaagttgag cctaaacagc tcgatacact attgcatcca     1140

aactttgaca gtgatgaatt gaaacgggca gttgtaatag caaatggact tcctgcatca     1200

ccaggagcag cttgtggtaa gatatatttt acagcagatg atgctaagaa acatcatgat     1260

caaggtgaaa aggtaatact tgtaaggcta gagacttctc cagaagatat agaaggaatg     1320

gcagcttctg aaggaatact tacagttaga ggaggcatga catctcatgc agctgttgta     1380

gcaagaggta tgggaacatg ctgtgtagct ggatgtggtg atcttatcgt aagtgaaaag     1440

gaaaagcttt tcaaaagatt agataaggtt tacaaagaag gggattacat atctttagat     1500

ggaagtactg gaaatgtata tggagagcct ataaagactg tagcaccaga aatatcagga     1560

gattttggaa tcttcatggg atgggctgac aatataagaa aattgggagt tagaacaaat     1620

gcagatacac caagagatgc aaaccaggct attagctttg gtgccgaagg aataggactt     1680

tgtagaacag agcatatgtt ctttgatgaa gatagaatac cagaaatgag ggaaatgata     1740

gtttcaaaaa cggaagagca gaggagaaaa gctttagata aattactacc aagacaaaag     1800

aaagatttta ttggaatata tgaggcaatg gaaggaaaac ctgtcacaat tagatttttg     1860

gatccaccac ttcatgaatt cttacctact gaaactgagg atatagagtc tttagccaag     1920

gaaatgggag taagttttca agaactaaaa gatactatag attctctaca tgaatttaat     1980

cctatgatgg ggcatagagg atgcaggctt actgtttcat atccagaaat agctgaaatg     2040

caaacaaggg ctattataga agcagctata gatgttaaaa agagaaaagg gtatgatata     2100

gttccagaaa ttatgatacc tcttgtagga gaaataaaag aattaaaata tgttaaagac     2160

gtagttgtga gggtagcaga tgaaataata caaaaagagg gaatcaattt aaaatatgaa     2220

gtaggaacta tgatagaaat tccaagagca gctattacag ctgatgaaat agctaaagaa     2280

gctgagttct tctcatttgg aactaatgat ttaactcaaa tgacttttgg attttcaaga     2340

gatgatgcag gtaaattttt gaatgattat tatgataaaa aagtatatga gtttgatcca     2400

ttccaaaggt tagatcaagt tggagtagga aaacttgtag agactgctgt aaaattaggt     2460

aaaaagacta gacctgacat tcatcttgga atatgtggag aacatggagg agatccatct     2520

tctgtagagt ttttccacaa tgtaggactt gactatgtat cttgttcacc atttagggta     2580

cctgtggcaa gacttgctgc agctcaggct cagataaaga atccaagacc aatcaaataa     2640


<210>  45
<211>  879
<212>  PRT
<213>  Clostridium autoethanogenum

<400>  45

Met Asn Gly Lys Lys Tyr Val Tyr Leu Phe Asn Glu Gly Asn Ala Gly 
1               5                   10                  15      


Met Arg Asn Leu Leu Gly Gly Lys Gly Ala Asn Leu Ala Glu Met Thr 
            20                  25                  30          


Asn Leu Gly Ile Pro Val Pro Gly Gly Phe Thr Ile Ser Thr Glu Ala 
        35                  40                  45              


Cys Thr Lys Tyr Tyr Glu Asp Gly Lys Ser Ile Ser Gln Gln Val Ile 
    50                  55                  60                  


Asp Gln Ile Tyr Asp Ala Leu Lys Asn Val Glu Glu Thr Thr Gly Lys 
65                  70                  75                  80  


Lys Phe Gly Ser Ile Glu Asn Pro Leu Leu Val Ser Val Arg Ser Gly 
                85                  90                  95      


Ala Arg Val Ser Met Pro Gly Met Met Asp Thr Ile Leu Asn Leu Gly 
            100                 105                 110         


Leu Asn Asp Asp Thr Val Ile Gly Leu Lys Lys Leu Thr Gly Asn Glu 
        115                 120                 125             


Arg Phe Ala Tyr Asp Ser Tyr Arg Arg Phe Ile Gln Met Phe Ser Asp 
    130                 135                 140                 


Val Val Met Gly Ile Glu Lys Arg Glu Phe Glu Asp Val Leu Asp Asp 
145                 150                 155                 160 


Val Lys Asn Ala Lys Gly Val Lys Tyr Asp Thr Asp Leu Asp Glu Ser 
                165                 170                 175     


Asp Leu Lys Asn Ile Ile Gln Arg Phe Lys Asp Ile Tyr Lys Lys Glu 
            180                 185                 190         


Val Lys Glu Asp Phe Pro Gln Asp Pro Lys Glu Gln Leu Ile Gln Ser 
        195                 200                 205             


Val Thr Ala Val Phe Arg Ser Trp Glu Asn Pro Arg Ala Ile Ile Tyr 
    210                 215                 220                 


Arg Arg Leu Asn Asp Ile Ser Gly Asp Trp Gly Thr Ala Val Asn Val 
225                 230                 235                 240 


Gln Ser Met Val Phe Gly Asn Met Gly Glu Thr Ser Gly Thr Gly Val 
                245                 250                 255     


Ala Phe Thr Arg Asn Pro Ser Thr Gly Glu Lys Ser Ile Phe Gly Glu 
            260                 265                 270         


Tyr Leu Ile Asn Ala Gln Gly Glu Asp Val Val Ala Gly Ile Arg Thr 
        275                 280                 285             


Pro Gln Pro Ile Thr Lys Leu Lys Glu Asp Leu Pro Glu Cys Tyr Ser 
    290                 295                 300                 


Gln Phe Met Ser Ile Ala Asn Lys Leu Glu Asn His Tyr Lys Asp Met 
305                 310                 315                 320 


Gln Asp Met Glu Phe Thr Ile Glu Gln Gly Lys Leu Tyr Phe Leu Gln 
                325                 330                 335     


Thr Arg Asn Gly Lys Arg Thr Ala Gln Ala Ala Leu Arg Ile Ala Val 
            340                 345                 350         


Asn Met Val Asp Glu Gly Leu Ile Thr Lys Glu Glu Ala Ile Leu Lys 
        355                 360                 365             


Val Glu Pro Lys Gln Leu Asp Thr Leu Leu His Pro Asn Phe Asp Ser 
    370                 375                 380                 


Asp Glu Leu Lys Arg Ala Val Val Ile Ala Asn Gly Leu Pro Ala Ser 
385                 390                 395                 400 


Pro Gly Ala Ala Cys Gly Lys Ile Tyr Phe Thr Ala Asp Asp Ala Lys 
                405                 410                 415     


Lys His His Asp Gln Gly Glu Lys Val Ile Leu Val Arg Leu Glu Thr 
            420                 425                 430         


Ser Pro Glu Asp Ile Glu Gly Met Ala Ala Ser Glu Gly Ile Leu Thr 
        435                 440                 445             


Val Arg Gly Gly Met Thr Ser His Ala Ala Val Val Ala Arg Gly Met 
    450                 455                 460                 


Gly Thr Cys Cys Val Ala Gly Cys Gly Asp Leu Ile Val Ser Glu Lys 
465                 470                 475                 480 


Glu Lys Leu Phe Lys Arg Leu Asp Lys Val Tyr Lys Glu Gly Asp Tyr 
                485                 490                 495     


Ile Ser Leu Asp Gly Ser Thr Gly Asn Val Tyr Gly Glu Pro Ile Lys 
            500                 505                 510         


Thr Val Ala Pro Glu Ile Ser Gly Asp Phe Gly Ile Phe Met Gly Trp 
        515                 520                 525             


Ala Asp Asn Ile Arg Lys Leu Gly Val Arg Thr Asn Ala Asp Thr Pro 
    530                 535                 540                 


Arg Asp Ala Asn Gln Ala Ile Ser Phe Gly Ala Glu Gly Ile Gly Leu 
545                 550                 555                 560 


Cys Arg Thr Glu His Met Phe Phe Asp Glu Asp Arg Ile Pro Glu Met 
                565                 570                 575     


Arg Glu Met Ile Val Ser Lys Thr Glu Glu Gln Arg Arg Lys Ala Leu 
            580                 585                 590         


Asp Lys Leu Leu Pro Arg Gln Lys Lys Asp Phe Ile Gly Ile Tyr Glu 
        595                 600                 605             


Ala Met Glu Gly Lys Pro Val Thr Ile Arg Phe Leu Asp Pro Pro Leu 
    610                 615                 620                 


His Glu Phe Leu Pro Thr Glu Thr Glu Asp Ile Glu Ser Leu Ala Lys 
625                 630                 635                 640 


Glu Met Gly Val Ser Phe Gln Glu Leu Lys Asp Thr Ile Asp Ser Leu 
                645                 650                 655     


His Glu Phe Asn Pro Met Met Gly His Arg Gly Cys Arg Leu Thr Val 
            660                 665                 670         


Ser Tyr Pro Glu Ile Ala Glu Met Gln Thr Arg Ala Ile Ile Glu Ala 
        675                 680                 685             


Ala Ile Asp Val Lys Lys Arg Lys Gly Tyr Asp Ile Val Pro Glu Ile 
    690                 695                 700                 


Met Ile Pro Leu Val Gly Glu Ile Lys Glu Leu Lys Tyr Val Lys Asp 
705                 710                 715                 720 


Val Val Val Arg Val Ala Asp Glu Ile Ile Gln Lys Glu Gly Ile Asn 
                725                 730                 735     


Leu Lys Tyr Glu Val Gly Thr Met Ile Glu Ile Pro Arg Ala Ala Ile 
            740                 745                 750         


Thr Ala Asp Glu Ile Ala Lys Glu Ala Glu Phe Phe Ser Phe Gly Thr 
        755                 760                 765             


Asn Asp Leu Thr Gln Met Thr Phe Gly Phe Ser Arg Asp Asp Ala Gly 
    770                 775                 780                 


Lys Phe Leu Asn Asp Tyr Tyr Asp Lys Lys Val Tyr Glu Phe Asp Pro 
785                 790                 795                 800 


Phe Gln Arg Leu Asp Gln Val Gly Val Gly Lys Leu Val Glu Thr Ala 
                805                 810                 815     


Val Lys Leu Gly Lys Lys Thr Arg Pro Asp Ile His Leu Gly Ile Cys 
            820                 825                 830         


Gly Glu His Gly Gly Asp Pro Ser Ser Val Glu Phe Phe His Asn Val 
        835                 840                 845             


Gly Leu Asp Tyr Val Ser Cys Ser Pro Phe Arg Val Pro Val Ala Arg 
    850                 855                 860                 


Leu Ala Ala Ala Gln Ala Gln Ile Lys Asn Pro Arg Pro Ile Lys 
865                 870                 875                 


<210>  46
<211>  3447
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  46
atggattact tgttaaagag gtttaagagg gttcttgtag cgaatagagg agaaatagcc       60

ataagaatat tcagagcatg caaagaattg ggaataacta ctgtagcaat atattcaaat      120

gaggataaga gatctctttt cagaactaaa gctgatgaat cctatatgat agggaaaaat      180

aagggacctg tagaagcata tttggatatt gatgaaataa tagatatagc tttgaagaaa      240

aatgtagatg caatacatcc gggttatgga ttcctatcag aaaatcctga attggcaaaa      300

aagtgtaaag aagcaggtat tgaatttata ggacctacat cagatatgat ggagatgctt      360

ggtgataaga taaaatctaa aattgttgca caaaaggctg gagtaccaac aataccagga      420

gttcaagaag ctataaagac agaagaagaa gctttaaaat ttgctaactt ctgtggatat      480

cctgttatga ttaaagcagc tgacggtggc ggcggcagag gaatgagaat agtaagggaa      540

gaaaaagatc tcatagaatc ctataacagt gctaaaaatg aatctagaaa agcttttggt      600

tcagaaaaaa tatacattga aaaatatatt gaaaatccaa aacacataga ggtgcaggta      660

cttggagata agtacggcaa tattgttcat ctatatgaaa gagattgttc catacagaga      720

agacatcaaa aggttataga atttacacca tctttagccc tctcagaaga aaaaagacaa      780

caaatatgtg aagatgcttt aaaaattgcg agaactgtag gatatacaag tgcaggtacc      840

ttggagtttt tagttgacaa aaatgaaaat cactatttta tagagatgaa tactagaatt      900

caggtagaac acactgtaac tgaaatggtt acaggaatag atatagttca agatcaaata      960

cttattgcag aagggcattc acttgattct aaggaaatag gaataaaatc tcaagatgat     1020

atagagttaa aaggatatgc aatacaatgc agaattacta cagaagatcc tttaaataat     1080

tttgcgccag atacaggaag aatagatatg tatagaactg gttctggatt tggtataaga     1140

cttgatggag gaaatggatt tacaggtgca gtaataagtc cttattatga tagcttactg     1200

gtaaaaactg tatcttggtc aagaactttt gaagatgcta taagaaaggc aataaggtct     1260

ataaatgaga ctgttatatc aggggtaaag acaaatgcag actttataat aaaagtgtta     1320

agtcatgaaa agtttataaa aggtgaatgt gatactaatt ttattgaaga taatccagat     1380

ttatttgata taaaaccaaa attagataaa gaaatgagtg tacttaaatt tataggaaat     1440

aaagtagtaa atgagactcg tggaaagaag aagaaattta atatacctat tgtaccaaaa     1500

gtagaagaaa atattaaatt gagtggaaca aagcaaatac ttgatacaaa aggagcagat     1560

ggattagttg attggataaa atcacaagat aagcttctta ttacagatac tactatgaga     1620

gacgctcatc agtcacttat ggcaactagg gtgagaacta gagatttgct taagatagca     1680

aaagcccaat cagctttggc aaacgatctt ttctccatgg aaatgtgggg aggagcaact     1740

tttgatgtag cgtatagatt tttaaatgaa tctccttggg aaagacttga aaaacttaga     1800

gaaaaggttc ctaatatact attccagatg ctcataagag gagctaatgc agtaggatat     1860

aagaactatc ctgataatgt tattagagaa tttataaaac aatcttcaac ttcaggcatt     1920

gatgtattta gaatatttga ttcactaaac tggcttaaag gaatgaaagt tgctatagat     1980

cagacattaa aagaaggaaa gatagctgaa gcatgcatgt gctatacagg agatgtatta     2040

gatgacaagg aggataaata tacgcttcag tactatataa acttagctaa agaaatagag     2100

aaaactggag cacagattct tggaataaag gatatgtctg ctttgttaaa gccatattct     2160

gcttataaac ttgtaaaagc acttaagaat gaaatctcta ttccaataca tcttcatact     2220

catgatacta caggtaatgg tgtggcaaca gtacttatgg ctgctgatgc aggacttgat     2280

atagctgata ctgcattcaa tagtatgtct gggcttacta gccagccagc tttgaattca     2340

atagcagcag cacttaaaaa tacaaataga gatactaagt tagatgcaga taatcttcaa     2400

aaaatatcta actactggga agatgtaaga cctatatata gtcagtttga gtcgggactt     2460

aagtcaagta ctgcagaaat atacaagtat gagataccag gaggccagta ttcaaactta     2520

aaacctcagg ttgaaagttt tgggttggga gatcgttttg aagatgtaaa agaaatgtat     2580

aagagagtta ataaaatgct tggaaatata attaaagtaa ctccttcttc aaaaatggta     2640

ggagatctgg ctatatttat gatacaaaat gatttggatg aaaagaatat ttatgaaaaa     2700

ggtaagaatt taactttccc agattctaca atttctttct tcaagggaat gatgggtcag     2760

cctatgggag gatttccaaa agaacttcaa aaaatagttc taaagggaga ggaaccttta     2820

aagggaagac caggagaatt tttgccacca gaagattttg gcaagataga agatcattta     2880

actaaaaaat ataagagaaa atttgaaaat aaagaactct tgagttatgc tatgtatcct     2940

gatgtatttg aaaattacct taaattcata gatgaatatg gtgatctcag cagaatggaa     3000

agtgaaacat tcttctatgg acttgcagaa ggagaacttt gtgaagttga aataggagaa     3060

ggaaaaagtt tatttgtaca attactagag attacaaaag ttgacgatga aggatataga     3120

ttcttagtgt ttgaggttaa tggtattaag agagacataa ggataaaaga taacttggct     3180

ttctctggat caggaataaa agaaaattca tgtgttatgg cagatgaaga taatgaaaaa     3240

gaaataggat caagtatacc tggaaacatt gttaaagtac ttgtaaaacc aggagataaa     3300

gtagaagagg gccagagctt aattgtaata gaagctatga aaatggaaac aaatgtttca     3360

gctgctgaag caggagtaat tgatggagta tttgtaaaag aaggccagag agttaaaact     3420

ggagaacttt taattaaatt aaagtaa                                         3447


<210>  47
<211>  1148
<212>  PRT
<213>  Clostridium autoethanogenum

<400>  47

Met Asp Tyr Leu Leu Lys Arg Phe Lys Arg Val Leu Val Ala Asn Arg 
1               5                   10                  15      


Gly Glu Ile Ala Ile Arg Ile Phe Arg Ala Cys Lys Glu Leu Gly Ile 
            20                  25                  30          


Thr Thr Val Ala Ile Tyr Ser Asn Glu Asp Lys Arg Ser Leu Phe Arg 
        35                  40                  45              


Thr Lys Ala Asp Glu Ser Tyr Met Ile Gly Lys Asn Lys Gly Pro Val 
    50                  55                  60                  


Glu Ala Tyr Leu Asp Ile Asp Glu Ile Ile Asp Ile Ala Leu Lys Lys 
65                  70                  75                  80  


Asn Val Asp Ala Ile His Pro Gly Tyr Gly Phe Leu Ser Glu Asn Pro 
                85                  90                  95      


Glu Leu Ala Lys Lys Cys Lys Glu Ala Gly Ile Glu Phe Ile Gly Pro 
            100                 105                 110         


Thr Ser Asp Met Met Glu Met Leu Gly Asp Lys Ile Lys Ser Lys Ile 
        115                 120                 125             


Val Ala Gln Lys Ala Gly Val Pro Thr Ile Pro Gly Val Gln Glu Ala 
    130                 135                 140                 


Ile Lys Thr Glu Glu Glu Ala Leu Lys Phe Ala Asn Phe Cys Gly Tyr 
145                 150                 155                 160 


Pro Val Met Ile Lys Ala Ala Asp Gly Gly Gly Gly Arg Gly Met Arg 
                165                 170                 175     


Ile Val Arg Glu Glu Lys Asp Leu Ile Glu Ser Tyr Asn Ser Ala Lys 
            180                 185                 190         


Asn Glu Ser Arg Lys Ala Phe Gly Ser Glu Lys Ile Tyr Ile Glu Lys 
        195                 200                 205             


Tyr Ile Glu Asn Pro Lys His Ile Glu Val Gln Val Leu Gly Asp Lys 
    210                 215                 220                 


Tyr Gly Asn Ile Val His Leu Tyr Glu Arg Asp Cys Ser Ile Gln Arg 
225                 230                 235                 240 


Arg His Gln Lys Val Ile Glu Phe Thr Pro Ser Leu Ala Leu Ser Glu 
                245                 250                 255     


Glu Lys Arg Gln Gln Ile Cys Glu Asp Ala Leu Lys Ile Ala Arg Thr 
            260                 265                 270         


Val Gly Tyr Thr Ser Ala Gly Thr Leu Glu Phe Leu Val Asp Lys Asn 
        275                 280                 285             


Glu Asn His Tyr Phe Ile Glu Met Asn Thr Arg Ile Gln Val Glu His 
    290                 295                 300                 


Thr Val Thr Glu Met Val Thr Gly Ile Asp Ile Val Gln Asp Gln Ile 
305                 310                 315                 320 


Leu Ile Ala Glu Gly His Ser Leu Asp Ser Lys Glu Ile Gly Ile Lys 
                325                 330                 335     


Ser Gln Asp Asp Ile Glu Leu Lys Gly Tyr Ala Ile Gln Cys Arg Ile 
            340                 345                 350         


Thr Thr Glu Asp Pro Leu Asn Asn Phe Ala Pro Asp Thr Gly Arg Ile 
        355                 360                 365             


Asp Met Tyr Arg Thr Gly Ser Gly Phe Gly Ile Arg Leu Asp Gly Gly 
    370                 375                 380                 


Asn Gly Phe Thr Gly Ala Val Ile Ser Pro Tyr Tyr Asp Ser Leu Leu 
385                 390                 395                 400 


Val Lys Thr Val Ser Trp Ser Arg Thr Phe Glu Asp Ala Ile Arg Lys 
                405                 410                 415     


Ala Ile Arg Ser Ile Asn Glu Thr Val Ile Ser Gly Val Lys Thr Asn 
            420                 425                 430         


Ala Asp Phe Ile Ile Lys Val Leu Ser His Glu Lys Phe Ile Lys Gly 
        435                 440                 445             


Glu Cys Asp Thr Asn Phe Ile Glu Asp Asn Pro Asp Leu Phe Asp Ile 
    450                 455                 460                 


Lys Pro Lys Leu Asp Lys Glu Met Ser Val Leu Lys Phe Ile Gly Asn 
465                 470                 475                 480 


Lys Val Val Asn Glu Thr Arg Gly Lys Lys Lys Lys Phe Asn Ile Pro 
                485                 490                 495     


Ile Val Pro Lys Val Glu Glu Asn Ile Lys Leu Ser Gly Thr Lys Gln 
            500                 505                 510         


Ile Leu Asp Thr Lys Gly Ala Asp Gly Leu Val Asp Trp Ile Lys Ser 
        515                 520                 525             


Gln Asp Lys Leu Leu Ile Thr Asp Thr Thr Met Arg Asp Ala His Gln 
    530                 535                 540                 


Ser Leu Met Ala Thr Arg Val Arg Thr Arg Asp Leu Leu Lys Ile Ala 
545                 550                 555                 560 


Lys Ala Gln Ser Ala Leu Ala Asn Asp Leu Phe Ser Met Glu Met Trp 
                565                 570                 575     


Gly Gly Ala Thr Phe Asp Val Ala Tyr Arg Phe Leu Asn Glu Ser Pro 
            580                 585                 590         


Trp Glu Arg Leu Glu Lys Leu Arg Glu Lys Val Pro Asn Ile Leu Phe 
        595                 600                 605             


Gln Met Leu Ile Arg Gly Ala Asn Ala Val Gly Tyr Lys Asn Tyr Pro 
    610                 615                 620                 


Asp Asn Val Ile Arg Glu Phe Ile Lys Gln Ser Ser Thr Ser Gly Ile 
625                 630                 635                 640 


Asp Val Phe Arg Ile Phe Asp Ser Leu Asn Trp Leu Lys Gly Met Lys 
                645                 650                 655     


Val Ala Ile Asp Gln Thr Leu Lys Glu Gly Lys Ile Ala Glu Ala Cys 
            660                 665                 670         


Met Cys Tyr Thr Gly Asp Val Leu Asp Asp Lys Glu Asp Lys Tyr Thr 
        675                 680                 685             


Leu Gln Tyr Tyr Ile Asn Leu Ala Lys Glu Ile Glu Lys Thr Gly Ala 
    690                 695                 700                 


Gln Ile Leu Gly Ile Lys Asp Met Ser Ala Leu Leu Lys Pro Tyr Ser 
705                 710                 715                 720 


Ala Tyr Lys Leu Val Lys Ala Leu Lys Asn Glu Ile Ser Ile Pro Ile 
                725                 730                 735     


His Leu His Thr His Asp Thr Thr Gly Asn Gly Val Ala Thr Val Leu 
            740                 745                 750         


Met Ala Ala Asp Ala Gly Leu Asp Ile Ala Asp Thr Ala Phe Asn Ser 
        755                 760                 765             


Met Ser Gly Leu Thr Ser Gln Pro Ala Leu Asn Ser Ile Ala Ala Ala 
    770                 775                 780                 


Leu Lys Asn Thr Asn Arg Asp Thr Lys Leu Asp Ala Asp Asn Leu Gln 
785                 790                 795                 800 


Lys Ile Ser Asn Tyr Trp Glu Asp Val Arg Pro Ile Tyr Ser Gln Phe 
                805                 810                 815     


Glu Ser Gly Leu Lys Ser Ser Thr Ala Glu Ile Tyr Lys Tyr Glu Ile 
            820                 825                 830         


Pro Gly Gly Gln Tyr Ser Asn Leu Lys Pro Gln Val Glu Ser Phe Gly 
        835                 840                 845             


Leu Gly Asp Arg Phe Glu Asp Val Lys Glu Met Tyr Lys Arg Val Asn 
    850                 855                 860                 


Lys Met Leu Gly Asn Ile Ile Lys Val Thr Pro Ser Ser Lys Met Val 
865                 870                 875                 880 


Gly Asp Leu Ala Ile Phe Met Ile Gln Asn Asp Leu Asp Glu Lys Asn 
                885                 890                 895     


Ile Tyr Glu Lys Gly Lys Asn Leu Thr Phe Pro Asp Ser Thr Ile Ser 
            900                 905                 910         


Phe Phe Lys Gly Met Met Gly Gln Pro Met Gly Gly Phe Pro Lys Glu 
        915                 920                 925             


Leu Gln Lys Ile Val Leu Lys Gly Glu Glu Pro Leu Lys Gly Arg Pro 
    930                 935                 940                 


Gly Glu Phe Leu Pro Pro Glu Asp Phe Gly Lys Ile Glu Asp His Leu 
945                 950                 955                 960 


Thr Lys Lys Tyr Lys Arg Lys Phe Glu Asn Lys Glu Leu Leu Ser Tyr 
                965                 970                 975     


Ala Met Tyr Pro Asp Val Phe Glu Asn Tyr Leu Lys Phe Ile Asp Glu 
            980                 985                 990         


Tyr Gly Asp Leu Ser Arg Met Glu  Ser Glu Thr Phe Phe  Tyr Gly Leu 
        995                 1000                 1005             


Ala Glu  Gly Glu Leu Cys Glu  Val Glu Ile Gly Glu  Gly Lys Ser 
    1010                 1015                 1020             


Leu Phe  Val Gln Leu Leu Glu  Ile Thr Lys Val Asp  Asp Glu Gly 
    1025                 1030                 1035             


Tyr Arg  Phe Leu Val Phe Glu  Val Asn Gly Ile Lys  Arg Asp Ile 
    1040                 1045                 1050             


Arg Ile  Lys Asp Asn Leu Ala  Phe Ser Gly Ser Gly  Ile Lys Glu 
    1055                 1060                 1065             


Asn Ser  Cys Val Met Ala Asp  Glu Asp Asn Glu Lys  Glu Ile Gly 
    1070                 1075                 1080             


Ser Ser  Ile Pro Gly Asn Ile  Val Lys Val Leu Val  Lys Pro Gly 
    1085                 1090                 1095             


Asp Lys  Val Glu Glu Gly Gln  Ser Leu Ile Val Ile  Glu Ala Met 
    1100                 1105                 1110             


Lys Met  Glu Thr Asn Val Ser  Ala Ala Glu Ala Gly  Val Ile Asp 
    1115                 1120                 1125             


Gly Val  Phe Val Lys Glu Gly  Gln Arg Val Lys Thr  Gly Glu Leu 
    1130                 1135                 1140             


Leu Ile  Lys Leu Lys 
    1145             


<210>  48
<211>  1524
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  48
atgtataaaa atttatcacc atcagaatta acggaatttt caattaaaag aggagaagga       60

tttttatcaa ataagggagc tcttatgatt aatactggaa agtacacagg aagatctcct      120

aaagatagat ttatagttaa tcaagaaagc attaggaaca aaataaactg gggaaatgta      180

aatctttcta tagaagaaga tatttttaat aaaatgtatg ataagatttt aaattatata      240

agtgataaag atatttttgt gtttgatgga tttgttggag ctttaaaaaa atataccctt      300

cctataagag taatatgcga aagggcatcc caggcgttgt ttgcaaatca attgtttaga      360

agaccaacgg aggaggattt aaagtgtttt actcctgaat ttaatattat atcggcacct      420

ggatttaagg ctaagggcaa agaagacggt ttaaattcag atgcctttat tttagtaaat      480

ttcgataaaa aaattatatt aataggtgga accagttact cgggagaaat aaaaaaatca      540

gtattttcag taatgaactt cttgcttcca caaaaaggag tcatgcctat gcactgttct      600

gctaatatag gacaagataa taaaacttgc ttattttttg gattgtcagg aacaggaaaa      660

actactttat cagcagatgg tgaaagaaga ctgattggtg atgacgaaca tggatggtct      720

aatgaaggtg tatttaattt tgagggtgga tgttatgcta aaactataag acttgataag      780

gaaaaggaaa gtcagatata caatgccata aaatttggaa ctgtagttga aaatgtagtg      840

gcagatggga atagagtacc tgattataat gatgctagat atactgaaaa tacaagggca      900

gcatatccta taaattatat agataatata gaagaaagtg gtgtaggagg aaatccagag      960

actataatat ttttaaccgc agatgctttt ggtgtaatgc cacctatatc aaggctttct     1020

aaagaagcag caatgtatca ctttatgtct ggatatacta gcaagatagc tggaactgaa     1080

agaggaataa ttgaacctca agctactttt tcctcttgct ttggtgaacc gtttatgtta     1140

atgaatcctg ctgtctatgc aaaattgtta ggcgaaagaa tagacaagta taacactcag     1200

gtatatttag tgaatactgg atggctatct ggaggatatg gaaatggaga tagaataaaa     1260

ctttcctata caagggctat gattagagaa gctttgaaag ggaagttcaa ggatgttgaa     1320

tttgtggaac atcctgtatt taaagtaatg atgcctaaaa gatgtccagg tgtacctgat     1380

gaaatattaa atcctagaaa tatatgggaa gataaagaag catatgatga gacagcgaga     1440

aagctggcgc tgaagtttag taaaaacttt gagaagttta aagatgtttc cgaagatata     1500

gcaaaagctg gacctgaagc ttaa                                            1524


<210>  49
<211>  507
<212>  PRT
<213>  Clostridium autoethanogenum

<400>  49

Met Tyr Lys Asn Leu Ser Pro Ser Glu Leu Thr Glu Phe Ser Ile Lys 
1               5                   10                  15      


Arg Gly Glu Gly Phe Leu Ser Asn Lys Gly Ala Leu Met Ile Asn Thr 
            20                  25                  30          


Gly Lys Tyr Thr Gly Arg Ser Pro Lys Asp Arg Phe Ile Val Asn Gln 
        35                  40                  45              


Glu Ser Ile Arg Asn Lys Ile Asn Trp Gly Asn Val Asn Leu Ser Ile 
    50                  55                  60                  


Glu Glu Asp Ile Phe Asn Lys Met Tyr Asp Lys Ile Leu Asn Tyr Ile 
65                  70                  75                  80  


Ser Asp Lys Asp Ile Phe Val Phe Asp Gly Phe Val Gly Ala Leu Lys 
                85                  90                  95      


Lys Tyr Thr Leu Pro Ile Arg Val Ile Cys Glu Arg Ala Ser Gln Ala 
            100                 105                 110         


Leu Phe Ala Asn Gln Leu Phe Arg Arg Pro Thr Glu Glu Asp Leu Lys 
        115                 120                 125             


Cys Phe Thr Pro Glu Phe Asn Ile Ile Ser Ala Pro Gly Phe Lys Ala 
    130                 135                 140                 


Lys Gly Lys Glu Asp Gly Leu Asn Ser Asp Ala Phe Ile Leu Val Asn 
145                 150                 155                 160 


Phe Asp Lys Lys Ile Ile Leu Ile Gly Gly Thr Ser Tyr Ser Gly Glu 
                165                 170                 175     


Ile Lys Lys Ser Val Phe Ser Val Met Asn Phe Leu Leu Pro Gln Lys 
            180                 185                 190         


Gly Val Met Pro Met His Cys Ser Ala Asn Ile Gly Gln Asp Asn Lys 
        195                 200                 205             


Thr Cys Leu Phe Phe Gly Leu Ser Gly Thr Gly Lys Thr Thr Leu Ser 
    210                 215                 220                 


Ala Asp Gly Glu Arg Arg Leu Ile Gly Asp Asp Glu His Gly Trp Ser 
225                 230                 235                 240 


Asn Glu Gly Val Phe Asn Phe Glu Gly Gly Cys Tyr Ala Lys Thr Ile 
                245                 250                 255     


Arg Leu Asp Lys Glu Lys Glu Ser Gln Ile Tyr Asn Ala Ile Lys Phe 
            260                 265                 270         


Gly Thr Val Val Glu Asn Val Val Ala Asp Gly Asn Arg Val Pro Asp 
        275                 280                 285             


Tyr Asn Asp Ala Arg Tyr Thr Glu Asn Thr Arg Ala Ala Tyr Pro Ile 
    290                 295                 300                 


Asn Tyr Ile Asp Asn Ile Glu Glu Ser Gly Val Gly Gly Asn Pro Glu 
305                 310                 315                 320 


Thr Ile Ile Phe Leu Thr Ala Asp Ala Phe Gly Val Met Pro Pro Ile 
                325                 330                 335     


Ser Arg Leu Ser Lys Glu Ala Ala Met Tyr His Phe Met Ser Gly Tyr 
            340                 345                 350         


Thr Ser Lys Ile Ala Gly Thr Glu Arg Gly Ile Ile Glu Pro Gln Ala 
        355                 360                 365             


Thr Phe Ser Ser Cys Phe Gly Glu Pro Phe Met Leu Met Asn Pro Ala 
    370                 375                 380                 


Val Tyr Ala Lys Leu Leu Gly Glu Arg Ile Asp Lys Tyr Asn Thr Gln 
385                 390                 395                 400 


Val Tyr Leu Val Asn Thr Gly Trp Leu Ser Gly Gly Tyr Gly Asn Gly 
                405                 410                 415     


Asp Arg Ile Lys Leu Ser Tyr Thr Arg Ala Met Ile Arg Glu Ala Leu 
            420                 425                 430         


Lys Gly Lys Phe Lys Asp Val Glu Phe Val Glu His Pro Val Phe Lys 
        435                 440                 445             


Val Met Met Pro Lys Arg Cys Pro Gly Val Pro Asp Glu Ile Leu Asn 
    450                 455                 460                 


Pro Arg Asn Ile Trp Glu Asp Lys Glu Ala Tyr Asp Glu Thr Ala Arg 
465                 470                 475                 480 


Lys Leu Ala Leu Lys Phe Ser Lys Asn Phe Glu Lys Phe Lys Asp Val 
                485                 490                 495     


Ser Glu Asp Ile Ala Lys Ala Gly Pro Glu Ala 
            500                 505         


<210>  50
<211>  843
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  50
atgagagaag tagatgtatc cactataact aaagctgtta gaaatctctg tatagatgcc       60

aattattatc tttcggagga tgttaagaaa aagataaaag aatgtgaaga ggacgaaaaa      120

tggcctactg caaaagacat tttaggtaaa atacttgaaa atatagatat atctaaaaat      180

gaagatgtgc ctatgtgtca agatacagga atggcttgtg tatttataac aattggccag      240

gatgttcata tagtaggagg aagtttagaa gacgcaataa ataagggagt aggccagggg      300

tatgtagaag ggtatttaag aaaatctgta gtctctgatc ctataaatag agttaatact      360

aaggacaata ctcctgcagt aatatattat gaaatagttc caggagataa acttaacata      420

aaagtggctc ctaaaggatt tggatcagaa aatatgagtc agataaaaat gcttaaacca      480

gcagatggtc ttaagggtgt taaagatttt gtaataaaag tagtaaagga cgcaggacca      540

aatccatgtc ctcctatggt tgtaggagta ggtataggag gaacttttga caaggctgca      600

aatcttgcaa agaaagctct tgtaagacca ttatctgaaa gaaataaaaa taagttttat      660

tcagatttag aaaatgaact tttagacaaa ataaatctcc ttggtatagg acctcaagga      720

ctagggggaa agactacagc tcttgcagta aatatagaaa cttatcctac ccatatagca      780

ggattacctg tagccgtaaa tataaattgt cacgttacaa gacataagga aatagaattg      840

taa                                                                    843


<210>  51
<211>  280
<212>  PRT
<213>  Clostridium autoethanogenum

<400>  51

Met Arg Glu Val Asp Val Ser Thr Ile Thr Lys Ala Val Arg Asn Leu 
1               5                   10                  15      


Cys Ile Asp Ala Asn Tyr Tyr Leu Ser Glu Asp Val Lys Lys Lys Ile 
            20                  25                  30          


Lys Glu Cys Glu Glu Asp Glu Lys Trp Pro Thr Ala Lys Asp Ile Leu 
        35                  40                  45              


Gly Lys Ile Leu Glu Asn Ile Asp Ile Ser Lys Asn Glu Asp Val Pro 
    50                  55                  60                  


Met Cys Gln Asp Thr Gly Met Ala Cys Val Phe Ile Thr Ile Gly Gln 
65                  70                  75                  80  


Asp Val His Ile Val Gly Gly Ser Leu Glu Asp Ala Ile Asn Lys Gly 
                85                  90                  95      


Val Gly Gln Gly Tyr Val Glu Gly Tyr Leu Arg Lys Ser Val Val Ser 
            100                 105                 110         


Asp Pro Ile Asn Arg Val Asn Thr Lys Asp Asn Thr Pro Ala Val Ile 
        115                 120                 125             


Tyr Tyr Glu Ile Val Pro Gly Asp Lys Leu Asn Ile Lys Val Ala Pro 
    130                 135                 140                 


Lys Gly Phe Gly Ser Glu Asn Met Ser Gln Ile Lys Met Leu Lys Pro 
145                 150                 155                 160 


Ala Asp Gly Leu Lys Gly Val Lys Asp Phe Val Ile Lys Val Val Lys 
                165                 170                 175     


Asp Ala Gly Pro Asn Pro Cys Pro Pro Met Val Val Gly Val Gly Ile 
            180                 185                 190         


Gly Gly Thr Phe Asp Lys Ala Ala Asn Leu Ala Lys Lys Ala Leu Val 
        195                 200                 205             


Arg Pro Leu Ser Glu Arg Asn Lys Asn Lys Phe Tyr Ser Asp Leu Glu 
    210                 215                 220                 


Asn Glu Leu Leu Asp Lys Ile Asn Leu Leu Gly Ile Gly Pro Gln Gly 
225                 230                 235                 240 


Leu Gly Gly Lys Thr Thr Ala Leu Ala Val Asn Ile Glu Thr Tyr Pro 
                245                 250                 255     


Thr His Ile Ala Gly Leu Pro Val Ala Val Asn Ile Asn Cys His Val 
            260                 265                 270         


Thr Arg His Lys Glu Ile Glu Leu 
        275                 280 


<210>  52
<211>  573
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  52
atgtatatgg aaaaaaagat aactactccg ttaacggaag aaaaggttaa aactttaaaa       60

gcaggggata gtgttttaat atcagggaca atatatactg ctagagatgc tgctcataag      120

agattagttg aattattaga tgaaggtaaa tcacttccta taaatgtaaa agatgaaata      180

atatattacg caggaccaag tcctgcaaaa ccaggccatg taataggttc agcaggacca      240

acaagtagtt atagaatgga tccatttgca ccaagactgc ttgatatagg tttaaaggga      300

atgataggaa aaggccttcg ttcaaaagaa gttatagaat ccatgaagaa aaataaagct      360

gtttactttg ctgcaatagg cggggctgca gcacttgtag caaaatccat aaagaaagca      420

gaagtagtag cttatgaaga tttggattct gaagctataa gaaaattaga agtaaaagac      480

ttacctgtaa ttgtagtaat agattcagag ggcaataatt tatatgaatc aggacgaaaa      540

gagtacttgg actctgtgga ccagtctaag tag                                   573


<210>  53
<211>  190
<212>  PRT
<213>  Clostridium autoethanogenum

<400>  53

Met Tyr Met Glu Lys Lys Ile Thr Thr Pro Leu Thr Glu Glu Lys Val 
1               5                   10                  15      


Lys Thr Leu Lys Ala Gly Asp Ser Val Leu Ile Ser Gly Thr Ile Tyr 
            20                  25                  30          


Thr Ala Arg Asp Ala Ala His Lys Arg Leu Val Glu Leu Leu Asp Glu 
        35                  40                  45              


Gly Lys Ser Leu Pro Ile Asn Val Lys Asp Glu Ile Ile Tyr Tyr Ala 
    50                  55                  60                  


Gly Pro Ser Pro Ala Lys Pro Gly His Val Ile Gly Ser Ala Gly Pro 
65                  70                  75                  80  


Thr Ser Ser Tyr Arg Met Asp Pro Phe Ala Pro Arg Leu Leu Asp Ile 
                85                  90                  95      


Gly Leu Lys Gly Met Ile Gly Lys Gly Leu Arg Ser Lys Glu Val Ile 
            100                 105                 110         


Glu Ser Met Lys Lys Asn Lys Ala Val Tyr Phe Ala Ala Ile Gly Gly 
        115                 120                 125             


Ala Ala Ala Leu Val Ala Lys Ser Ile Lys Lys Ala Glu Val Val Ala 
    130                 135                 140                 


Tyr Glu Asp Leu Asp Ser Glu Ala Ile Arg Lys Leu Glu Val Lys Asp 
145                 150                 155                 160 


Leu Pro Val Ile Val Val Ile Asp Ser Glu Gly Asn Asn Leu Tyr Glu 
                165                 170                 175     


Ser Gly Arg Lys Glu Tyr Leu Asp Ser Val Asp Gln Ser Lys 
            180                 185                 190 


<210>  54
<211>  1797
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  54
atgcaaatag ataagataat tgatactgac atattagttg ttggaggttc tggagcagga       60

tcaatggcag ctgtaacagc tgcgagaaaa ggagcaaaag tactacttgc agtaaaaggg      120

aagcttggaa aaagcggaaa tgccattatg gcaggggcag gattttctat ggatggagaa      180

acagcatatt ataaatatgg actcaaggaa gcagatccta aaaatacaaa gggaaaatta      240

tttgaacaaa ttgtaaaaca gtctttttat ttaagtgatc aaaatatggt tgagcagttt      300

gtaagtgatt gtgatgagtg ctgctgggaa cttaaacagt ggattgaaaa agcaggacac      360

aaggttgtat tttttggaga agaaggatat ataagctcgg gtaaagctgt gggagttggc      420

tgccgatatg gagtttcaaa agcaggtggt attgatgtta tagaagattt tatagcagtg      480

gatattttaa tggaagataa aaaagctgta ggtgcagtgg gcatagaagt atatacagga      540

aaaattattg aaatcagatc aaagtcagtt attttagcta ctggcggata tcagccttat      600

tcctttaaat gtactgtttc cgatatgact ggcgatggaa tggctatggc ataccgtgca      660

ggagccaagc ttgcagatat ggaattttta ttatatatac cagcagttgc cctttcacca      720

tcagtatata aaggttcaat ttatcccttc ttacactcca gtatgttaat gcctattgtt      780

aaaaatggta agggagaatc aattttagat aatatacctg aaaatttact taaaatggcc      840

aaggaaagtg aaatgggaaa gcttatattt acgtattatt atggagatca aattgcaaga      900

ggaaaaggaa ctccaaatgg aggagtatat tttgattatt ccaatgtacc ttttgatatt      960

tatgaaaaag ctttaaaaaa agctgaacca ttaatgaaca tatggtatag aaaaggattc     1020

tatcaaggaa acaacttgga tacttttgtt gaaaatatta gaaagggcat tccatgggaa     1080

gtaggtattg gcagtgaata cagcatgggc ggcattgaag tagacgaaaa tatgtacact     1140

ggagtaccag gactttatgc agctggtgag actacaagtg gtgtatttgg agctatgagg     1200

gttgcagatg gacttattga aatgcttgta cagggttata gagcagcatt gtccgcttgc     1260

aaatatatac aaaatgtaaa tgagccaagt atgaaaaata ccaatattga tagtataatt     1320

aaagatattt tttcacctct tgaaagaaaa gaaggagtta gtcctataaa aatacacaga     1380

aatatagaaa aaacggctga tgttggattc aactttagaa gaaatgaaga gggacttaca     1440

aaagctttag atgaaatttt aaaaatacac aaatatgaca taagcgcaat gagtactaaa     1500

agtaaaaata gagtttataa ctatgaatgg atagaatcag tacaggctcg aaatctttta     1560

acttgtacag aagcaggtgt aagagctgcc cttatgagaa aggaaagtag gggtacacac     1620

atacgtgatg attatgaatt tgtagataat gataactggc ttttaaggat tatgagttcg     1680

aaaggtgaag acgaaaccat gaaattatca accagaaagc ctaaagtaac aacaatggaa     1740

cttccaggtg gtaaaaataa gaatattcct gattatatgc tttcaatgtt aaagtaa        1797


<210>  55
<211>  598
<212>  PRT
<213>  Clostridium autoethanogenum

<400>  55

Met Gln Ile Asp Lys Ile Ile Asp Thr Asp Ile Leu Val Val Gly Gly 
1               5                   10                  15      


Ser Gly Ala Gly Ser Met Ala Ala Val Thr Ala Ala Arg Lys Gly Ala 
            20                  25                  30          


Lys Val Leu Leu Ala Val Lys Gly Lys Leu Gly Lys Ser Gly Asn Ala 
        35                  40                  45              


Ile Met Ala Gly Ala Gly Phe Ser Met Asp Gly Glu Thr Ala Tyr Tyr 
    50                  55                  60                  


Lys Tyr Gly Leu Lys Glu Ala Asp Pro Lys Asn Thr Lys Gly Lys Leu 
65                  70                  75                  80  


Phe Glu Gln Ile Val Lys Gln Ser Phe Tyr Leu Ser Asp Gln Asn Met 
                85                  90                  95      


Val Glu Gln Phe Val Ser Asp Cys Asp Glu Cys Cys Trp Glu Leu Lys 
            100                 105                 110         


Gln Trp Ile Glu Lys Ala Gly His Lys Val Val Phe Phe Gly Glu Glu 
        115                 120                 125             


Gly Tyr Ile Ser Ser Gly Lys Ala Val Gly Val Gly Cys Arg Tyr Gly 
    130                 135                 140                 


Val Ser Lys Ala Gly Gly Ile Asp Val Ile Glu Asp Phe Ile Ala Val 
145                 150                 155                 160 


Asp Ile Leu Met Glu Asp Lys Lys Ala Val Gly Ala Val Gly Ile Glu 
                165                 170                 175     


Val Tyr Thr Gly Lys Ile Ile Glu Ile Arg Ser Lys Ser Val Ile Leu 
            180                 185                 190         


Ala Thr Gly Gly Tyr Gln Pro Tyr Ser Phe Lys Cys Thr Val Ser Asp 
        195                 200                 205             


Met Thr Gly Asp Gly Met Ala Met Ala Tyr Arg Ala Gly Ala Lys Leu 
    210                 215                 220                 


Ala Asp Met Glu Phe Leu Leu Tyr Ile Pro Ala Val Ala Leu Ser Pro 
225                 230                 235                 240 


Ser Val Tyr Lys Gly Ser Ile Tyr Pro Phe Leu His Ser Ser Met Leu 
                245                 250                 255     


Met Pro Ile Val Lys Asn Gly Lys Gly Glu Ser Ile Leu Asp Asn Ile 
            260                 265                 270         


Pro Glu Asn Leu Leu Lys Met Ala Lys Glu Ser Glu Met Gly Lys Leu 
        275                 280                 285             


Ile Phe Thr Tyr Tyr Tyr Gly Asp Gln Ile Ala Arg Gly Lys Gly Thr 
    290                 295                 300                 


Pro Asn Gly Gly Val Tyr Phe Asp Tyr Ser Asn Val Pro Phe Asp Ile 
305                 310                 315                 320 


Tyr Glu Lys Ala Leu Lys Lys Ala Glu Pro Leu Met Asn Ile Trp Tyr 
                325                 330                 335     


Arg Lys Gly Phe Tyr Gln Gly Asn Asn Leu Asp Thr Phe Val Glu Asn 
            340                 345                 350         


Ile Arg Lys Gly Ile Pro Trp Glu Val Gly Ile Gly Ser Glu Tyr Ser 
        355                 360                 365             


Met Gly Gly Ile Glu Val Asp Glu Asn Met Tyr Thr Gly Val Pro Gly 
    370                 375                 380                 


Leu Tyr Ala Ala Gly Glu Thr Thr Ser Gly Val Phe Gly Ala Met Arg 
385                 390                 395                 400 


Val Ala Asp Gly Leu Ile Glu Met Leu Val Gln Gly Tyr Arg Ala Ala 
                405                 410                 415     


Leu Ser Ala Cys Lys Tyr Ile Gln Asn Val Asn Glu Pro Ser Met Lys 
            420                 425                 430         


Asn Thr Asn Ile Asp Ser Ile Ile Lys Asp Ile Phe Ser Pro Leu Glu 
        435                 440                 445             


Arg Lys Glu Gly Val Ser Pro Ile Lys Ile His Arg Asn Ile Glu Lys 
    450                 455                 460                 


Thr Ala Asp Val Gly Phe Asn Phe Arg Arg Asn Glu Glu Gly Leu Thr 
465                 470                 475                 480 


Lys Ala Leu Asp Glu Ile Leu Lys Ile His Lys Tyr Asp Ile Ser Ala 
                485                 490                 495     


Met Ser Thr Lys Ser Lys Asn Arg Val Tyr Asn Tyr Glu Trp Ile Glu 
            500                 505                 510         


Ser Val Gln Ala Arg Asn Leu Leu Thr Cys Thr Glu Ala Gly Val Arg 
        515                 520                 525             


Ala Ala Leu Met Arg Lys Glu Ser Arg Gly Thr His Ile Arg Asp Asp 
    530                 535                 540                 


Tyr Glu Phe Val Asp Asn Asp Asn Trp Leu Leu Arg Ile Met Ser Ser 
545                 550                 555                 560 


Lys Gly Glu Asp Glu Thr Met Lys Leu Ser Thr Arg Lys Pro Lys Val 
                565                 570                 575     


Thr Thr Met Glu Leu Pro Gly Gly Lys Asn Lys Asn Ile Pro Asp Tyr 
            580                 585                 590         


Met Leu Ser Met Leu Lys 
        595             


<210>  56
<211>  2781
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  56
atgctaacta ataatactga ggcacttata ttggcagaaa agattggaaa agaagctgca       60

ctatcatgtt tacagggaat gtatgattat ggcacaagtc ctatgaaagt tttagatatg      120

ataaaggaaa aacctaattg taaagttata gttggagcac taaacaattc ggatacagat      180

ggaatgctta atattttggc aaaaacaaat ccagctggac ttgcagcagg tctttcagta      240

ttagcaaagg catcaggagc agaagaggcc ctattagaac ttcgtaatac ggataatgaa      300

gctgaattat tagccagtgc aaaaacagca ggagtaaaac ttagagtaga agtaggtgaa      360

ctagttgatg taagggcaca taaagagcat attattttta atttagaaac tttagccgga      420

attgccgata aaattacggg cacagcacca ggaattatta tagcagtaga tgaagatgta      480

cctaaggaag ttaaattcgg tacaaaactt gtggattttt tagacacagc taaggttaaa      540

tcggttatga ttaaccacca tttttataga ttagatgtat taaataatgg cattataaag      600

gaaaactcct atggaagtgg tgttattcat ataatttacg aaaatgattg catagtagaa      660

aaaacaaaaa aagaactaga aaatcttaga aagcaaagct gtggaaaatg tactttttgc      720

cgtgaaggat tatatcagct tgatgttata tttgatgaca tgataaaagg cagatcagag      780

aaagaggatc ttgctatggt agaagagtta acaagtgcaa tgaaattttc atgtaattgc      840

tcattaggaa aatgctcagg agaaccagca gcaagtgcaa taacagaatt taaattagaa      900

gtagagcagc atataaagaa gaggggatgt cctgctggcc agtgtttagc ctttacaaat      960

atatacgtag atcctagaaa atgcaaaggt tgtggaaaat gcttggaagt ttgtccagag     1020

gattgtattg aagcaaaaaa gggctatatt tccatgattg acgaatttga ttgcactaaa     1080

tgcggcatat gtatagatga atgtcccaat aatgcaattg ttaaggtaaa tggcaaaacc     1140

cctaaacttc ctacaaaact aacaagagta aaaggaagta aaaatataac agaagaggac     1200

acagagaaga aaaaacggca caatttaaaa agacacagaa ctaagcttgt tattccactt     1260

aaaaaagata ataataaagc atctgagaaa atatcagaga ttaaaaagtc aaaggagggt     1320

actattatga aaaagatgga aacagatatt ataatcgcag caggaggccc agcaggactg     1380

gcagcagcta ttacagctgg agagaacaat ttaaaatcta ttctttttga aaaatctagt     1440

acaacaggtg gagcagcaaa catgggtatg ggaccacttg gaatagatac taaaattcag     1500

aaagataact ttaacaatat aagtgtagca gaagcccttg acatgcatat gaaatatact     1560

cattatcgtg tagatgagga tttagttcag acatacttta ataagagtgc agaaacaatt     1620

gaatggttac aggatatggg agtagaattt gcaggagcat ttcgctattt taaagaatca     1680

gcggcaactt ggcatatagt taagccggaa aatggagtta ttggaccacg tgcagctagt     1740

ggaatggcaa aaataatgac agaacgtgca aaagaacttg gaacaaaaat cctattggaa     1800

acaccagtgg tttctttaat aaaggaaaat ggaagaatat gtggtgttaa agcacaagat     1860

agtgaaggta atattattga agtcagggca aaagctgtta ttgttgcaac tggtggtttt     1920

ggcaataaca agaatatgat aaaatctgaa tttggtttga caattggaga agattatttc     1980

ccatttatgg ttcctggaat aacaggtgat ggcttgaaaa tgatgtggga agcaggtgca     2040

atgaaatatg gagaaaatat tgaggcaatt tatcagcttc ctgataattt aaactggttt     2100

ttactagatg cagtgctgcg tcagccaaat cttttaatta atcaacttgg tgatcgtttt     2160

atgaatgaag gagatatggg aaatactaca tttactggaa atgccattgc aatgcagccg     2220

ggcaattatg cttactgcat tatggatgaa ggaattttaa aacattataa aaagaatggt     2280

ccagatattt ttgatattgt tcatccagca gatgctttcc ttgcagttga tggagagatt     2340

gctaaggcag tagaacaagg ttatgaatca tattttgaag cacgaacagt agaagagctt     2400

gctaaaaaac ttaatattga tgctgaaaaa ttacaagata ctattgatga atataatgaa     2460

gcctgtgaaa cgggagtaga cactaaattc cataaaaaac aggcatatct ccatcctatc     2520

actggaaagg gaaaatattt agttggaaaa ttctaccttg gagcttatgg aacaattggt     2580

ggtgttcgta tcaataaata ttgtgaagtt ttagatgaaa gctttaatcc aattgaggga     2640

ctttatagcg ctggtactga tgctaataca atttatggag acagctataa ttttactctt     2700

cctggtaaca gcatgggatt tgcaattaat tcaggacgta tggctggaga aagtgccgca     2760

gagtatattg aagaagtata a                                               2781


<210>  57
<211>  926
<212>  PRT
<213>  Clostridium autoethanogenum

<400>  57

Met Leu Thr Asn Asn Thr Glu Ala Leu Ile Leu Ala Glu Lys Ile Gly 
1               5                   10                  15      


Lys Glu Ala Ala Leu Ser Cys Leu Gln Gly Met Tyr Asp Tyr Gly Thr 
            20                  25                  30          


Ser Pro Met Lys Val Leu Asp Met Ile Lys Glu Lys Pro Asn Cys Lys 
        35                  40                  45              


Val Ile Val Gly Ala Leu Asn Asn Ser Asp Thr Asp Gly Met Leu Asn 
    50                  55                  60                  


Ile Leu Ala Lys Thr Asn Pro Ala Gly Leu Ala Ala Gly Leu Ser Val 
65                  70                  75                  80  


Leu Ala Lys Ala Ser Gly Ala Glu Glu Ala Leu Leu Glu Leu Arg Asn 
                85                  90                  95      


Thr Asp Asn Glu Ala Glu Leu Leu Ala Ser Ala Lys Thr Ala Gly Val 
            100                 105                 110         


Lys Leu Arg Val Glu Val Gly Glu Leu Val Asp Val Arg Ala His Lys 
        115                 120                 125             


Glu His Ile Ile Phe Asn Leu Glu Thr Leu Ala Gly Ile Ala Asp Lys 
    130                 135                 140                 


Ile Thr Gly Thr Ala Pro Gly Ile Ile Ile Ala Val Asp Glu Asp Val 
145                 150                 155                 160 


Pro Lys Glu Val Lys Phe Gly Thr Lys Leu Val Asp Phe Leu Asp Thr 
                165                 170                 175     


Ala Lys Val Lys Ser Val Met Ile Asn His His Phe Tyr Arg Leu Asp 
            180                 185                 190         


Val Leu Asn Asn Gly Ile Ile Lys Glu Asn Ser Tyr Gly Ser Gly Val 
        195                 200                 205             


Ile His Ile Ile Tyr Glu Asn Asp Cys Ile Val Glu Lys Thr Lys Lys 
    210                 215                 220                 


Glu Leu Glu Asn Leu Arg Lys Gln Ser Cys Gly Lys Cys Thr Phe Cys 
225                 230                 235                 240 


Arg Glu Gly Leu Tyr Gln Leu Asp Val Ile Phe Asp Asp Met Ile Lys 
                245                 250                 255     


Gly Arg Ser Glu Lys Glu Asp Leu Ala Met Val Glu Glu Leu Thr Ser 
            260                 265                 270         


Ala Met Lys Phe Ser Cys Asn Cys Ser Leu Gly Lys Cys Ser Gly Glu 
        275                 280                 285             


Pro Ala Ala Ser Ala Ile Thr Glu Phe Lys Leu Glu Val Glu Gln His 
    290                 295                 300                 


Ile Lys Lys Arg Gly Cys Pro Ala Gly Gln Cys Leu Ala Phe Thr Asn 
305                 310                 315                 320 


Ile Tyr Val Asp Pro Arg Lys Cys Lys Gly Cys Gly Lys Cys Leu Glu 
                325                 330                 335     


Val Cys Pro Glu Asp Cys Ile Glu Ala Lys Lys Gly Tyr Ile Ser Met 
            340                 345                 350         


Ile Asp Glu Phe Asp Cys Thr Lys Cys Gly Ile Cys Ile Asp Glu Cys 
        355                 360                 365             


Pro Asn Asn Ala Ile Val Lys Val Asn Gly Lys Thr Pro Lys Leu Pro 
    370                 375                 380                 


Thr Lys Leu Thr Arg Val Lys Gly Ser Lys Asn Ile Thr Glu Glu Asp 
385                 390                 395                 400 


Thr Glu Lys Lys Lys Arg His Asn Leu Lys Arg His Arg Thr Lys Leu 
                405                 410                 415     


Val Ile Pro Leu Lys Lys Asp Asn Asn Lys Ala Ser Glu Lys Ile Ser 
            420                 425                 430         


Glu Ile Lys Lys Ser Lys Glu Gly Thr Ile Met Lys Lys Met Glu Thr 
        435                 440                 445             


Asp Ile Ile Ile Ala Ala Gly Gly Pro Ala Gly Leu Ala Ala Ala Ile 
    450                 455                 460                 


Thr Ala Gly Glu Asn Asn Leu Lys Ser Ile Leu Phe Glu Lys Ser Ser 
465                 470                 475                 480 


Thr Thr Gly Gly Ala Ala Asn Met Gly Met Gly Pro Leu Gly Ile Asp 
                485                 490                 495     


Thr Lys Ile Gln Lys Asp Asn Phe Asn Asn Ile Ser Val Ala Glu Ala 
            500                 505                 510         


Leu Asp Met His Met Lys Tyr Thr His Tyr Arg Val Asp Glu Asp Leu 
        515                 520                 525             


Val Gln Thr Tyr Phe Asn Lys Ser Ala Glu Thr Ile Glu Trp Leu Gln 
    530                 535                 540                 


Asp Met Gly Val Glu Phe Ala Gly Ala Phe Arg Tyr Phe Lys Glu Ser 
545                 550                 555                 560 


Ala Ala Thr Trp His Ile Val Lys Pro Glu Asn Gly Val Ile Gly Pro 
                565                 570                 575     


Arg Ala Ala Ser Gly Met Ala Lys Ile Met Thr Glu Arg Ala Lys Glu 
            580                 585                 590         


Leu Gly Thr Lys Ile Leu Leu Glu Thr Pro Val Val Ser Leu Ile Lys 
        595                 600                 605             


Glu Asn Gly Arg Ile Cys Gly Val Lys Ala Gln Asp Ser Glu Gly Asn 
    610                 615                 620                 


Ile Ile Glu Val Arg Ala Lys Ala Val Ile Val Ala Thr Gly Gly Phe 
625                 630                 635                 640 


Gly Asn Asn Lys Asn Met Ile Lys Ser Glu Phe Gly Leu Thr Ile Gly 
                645                 650                 655     


Glu Asp Tyr Phe Pro Phe Met Val Pro Gly Ile Thr Gly Asp Gly Leu 
            660                 665                 670         


Lys Met Met Trp Glu Ala Gly Ala Met Lys Tyr Gly Glu Asn Ile Glu 
        675                 680                 685             


Ala Ile Tyr Gln Leu Pro Asp Asn Leu Asn Trp Phe Leu Leu Asp Ala 
    690                 695                 700                 


Val Leu Arg Gln Pro Asn Leu Leu Ile Asn Gln Leu Gly Asp Arg Phe 
705                 710                 715                 720 


Met Asn Glu Gly Asp Met Gly Asn Thr Thr Phe Thr Gly Asn Ala Ile 
                725                 730                 735     


Ala Met Gln Pro Gly Asn Tyr Ala Tyr Cys Ile Met Asp Glu Gly Ile 
            740                 745                 750         


Leu Lys His Tyr Lys Lys Asn Gly Pro Asp Ile Phe Asp Ile Val His 
        755                 760                 765             


Pro Ala Asp Ala Phe Leu Ala Val Asp Gly Glu Ile Ala Lys Ala Val 
    770                 775                 780                 


Glu Gln Gly Tyr Glu Ser Tyr Phe Glu Ala Arg Thr Val Glu Glu Leu 
785                 790                 795                 800 


Ala Lys Lys Leu Asn Ile Asp Ala Glu Lys Leu Gln Asp Thr Ile Asp 
                805                 810                 815     


Glu Tyr Asn Glu Ala Cys Glu Thr Gly Val Asp Thr Lys Phe His Lys 
            820                 825                 830         


Lys Gln Ala Tyr Leu His Pro Ile Thr Gly Lys Gly Lys Tyr Leu Val 
        835                 840                 845             


Gly Lys Phe Tyr Leu Gly Ala Tyr Gly Thr Ile Gly Gly Val Arg Ile 
    850                 855                 860                 


Asn Lys Tyr Cys Glu Val Leu Asp Glu Ser Phe Asn Pro Ile Glu Gly 
865                 870                 875                 880 


Leu Tyr Ser Ala Gly Thr Asp Ala Asn Thr Ile Tyr Gly Asp Ser Tyr 
                885                 890                 895     


Asn Phe Thr Leu Pro Gly Asn Ser Met Gly Phe Ala Ile Asn Ser Gly 
            900                 905                 910         


Arg Met Ala Gly Glu Ser Ala Ala Glu Tyr Ile Glu Glu Val 
        915                 920                 925     


<210>  58
<211>  1461
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  58
atgagagaat ttgaaacaga tgtagttgtt gttggtggag gagcatcagg gctagctgca       60

gcagttactg ctgcagaaaa tggtgcaaaa gtaatggtgc ttgaaaaagc taatactaca      120

ggtggatgtg ctaatatggc aatggggcct ctaggtgttg aaacaagaat gcaaagagaa      180

agacttatag atatctctgt agatagagct tttaataagt tcatggaata ttctcactgg      240

agatcagatg caagattgat aagaagatat ttggagcagt cagcaggaac tattgaatgg      300

ttagaaaata tgggagtaga attcgcatta ccttcaaagt attttccagc ttcagaagca      360

acctggcata ttgttaaacc taaaacaggg aaaccgggac ttcgtgcagc tgctactatg      420

attaaaatta tgacagaaag agcagaagaa ttaggcgtta aaatattatt agaaacacct      480

gtaaaaagta ttattaaaga tcaaggagaa gtaattggcg taacagctag tgataaagat      540

ggtgaattag aagtatatgc tggagcagtt atcatcggta caggtggatt tggtgataat      600

ccagacttta ttaagaagta tgttggactt gaatggggaa aagatttgtt ctcatataga      660

attcctggat taactggaga tggaatccag atggcttggg atgctggtgc ttcaaaagat      720

tttatgacta tggaaatggt attttttgct cctaacactg gtggatatgc tcctatagag      780

ttacctttcc gtcaacctaa tctcttagtt aacctggacg gtgaaagatt tataaatgaa      840

gaagttatag aaaatcctgt atttaccgca aatgctattg aaaaacaaaa aagaaaaatt      900

gcatattcta taatagatga agaactaatc aagcattatg aagaaaaggg cttagatctt      960

ataaatgtgg ttacttctag tatggatatg agttatttta gacaagaaga agaagaagct     1020

aagaaaaatg gaagtgatgt attatttatt gctgattcta tagaagagtt agctgaaaaa     1080

actggcattg atgcagaaaa cttaaaaaat accattgata cttataattc ttattgtgat     1140

tcaaaagatg agttattcca taaaaatcct aaatacttat taccaattaa aggctctaaa     1200

tattacgcat taaaacttgg tttaagtgca tatggaagtg ctggcggtat aaaaataaac     1260

tataatactg aagttcttaa tgatgattta aatgttataa agggacttta tgctgctgga     1320

actgatgcta attcactata taatcctgat tatgcttttg tactacctgg aaattcccta     1380

ggttttgctt tgaacagcgg aagaatagca gggtctagcg ctgttgaata tattaaagca     1440

aatcttatgg aagaacaata a                                               1461


<210>  59
<211>  486
<212>  PRT
<213>  Clostridium autoethanogenum

<400>  59

Met Arg Glu Phe Glu Thr Asp Val Val Val Val Gly Gly Gly Ala Ser 
1               5                   10                  15      


Gly Leu Ala Ala Ala Val Thr Ala Ala Glu Asn Gly Ala Lys Val Met 
            20                  25                  30          


Val Leu Glu Lys Ala Asn Thr Thr Gly Gly Cys Ala Asn Met Ala Met 
        35                  40                  45              


Gly Pro Leu Gly Val Glu Thr Arg Met Gln Arg Glu Arg Leu Ile Asp 
    50                  55                  60                  


Ile Ser Val Asp Arg Ala Phe Asn Lys Phe Met Glu Tyr Ser His Trp 
65                  70                  75                  80  


Arg Ser Asp Ala Arg Leu Ile Arg Arg Tyr Leu Glu Gln Ser Ala Gly 
                85                  90                  95      


Thr Ile Glu Trp Leu Glu Asn Met Gly Val Glu Phe Ala Leu Pro Ser 
            100                 105                 110         


Lys Tyr Phe Pro Ala Ser Glu Ala Thr Trp His Ile Val Lys Pro Lys 
        115                 120                 125             


Thr Gly Lys Pro Gly Leu Arg Ala Ala Ala Thr Met Ile Lys Ile Met 
    130                 135                 140                 


Thr Glu Arg Ala Glu Glu Leu Gly Val Lys Ile Leu Leu Glu Thr Pro 
145                 150                 155                 160 


Val Lys Ser Ile Ile Lys Asp Gln Gly Glu Val Ile Gly Val Thr Ala 
                165                 170                 175     


Ser Asp Lys Asp Gly Glu Leu Glu Val Tyr Ala Gly Ala Val Ile Ile 
            180                 185                 190         


Gly Thr Gly Gly Phe Gly Asp Asn Pro Asp Phe Ile Lys Lys Tyr Val 
        195                 200                 205             


Gly Leu Glu Trp Gly Lys Asp Leu Phe Ser Tyr Arg Ile Pro Gly Leu 
    210                 215                 220                 


Thr Gly Asp Gly Ile Gln Met Ala Trp Asp Ala Gly Ala Ser Lys Asp 
225                 230                 235                 240 


Phe Met Thr Met Glu Met Val Phe Phe Ala Pro Asn Thr Gly Gly Tyr 
                245                 250                 255     


Ala Pro Ile Glu Leu Pro Phe Arg Gln Pro Asn Leu Leu Val Asn Leu 
            260                 265                 270         


Asp Gly Glu Arg Phe Ile Asn Glu Glu Val Ile Glu Asn Pro Val Phe 
        275                 280                 285             


Thr Ala Asn Ala Ile Glu Lys Gln Lys Arg Lys Ile Ala Tyr Ser Ile 
    290                 295                 300                 


Ile Asp Glu Glu Leu Ile Lys His Tyr Glu Glu Lys Gly Leu Asp Leu 
305                 310                 315                 320 


Ile Asn Val Val Thr Ser Ser Met Asp Met Ser Tyr Phe Arg Gln Glu 
                325                 330                 335     


Glu Glu Glu Ala Lys Lys Asn Gly Ser Asp Val Leu Phe Ile Ala Asp 
            340                 345                 350         


Ser Ile Glu Glu Leu Ala Glu Lys Thr Gly Ile Asp Ala Glu Asn Leu 
        355                 360                 365             


Lys Asn Thr Ile Asp Thr Tyr Asn Ser Tyr Cys Asp Ser Lys Asp Glu 
    370                 375                 380                 


Leu Phe His Lys Asn Pro Lys Tyr Leu Leu Pro Ile Lys Gly Ser Lys 
385                 390                 395                 400 


Tyr Tyr Ala Leu Lys Leu Gly Leu Ser Ala Tyr Gly Ser Ala Gly Gly 
                405                 410                 415     


Ile Lys Ile Asn Tyr Asn Thr Glu Val Leu Asn Asp Asp Leu Asn Val 
            420                 425                 430         


Ile Lys Gly Leu Tyr Ala Ala Gly Thr Asp Ala Asn Ser Leu Tyr Asn 
        435                 440                 445             


Pro Asp Tyr Ala Phe Val Leu Pro Gly Asn Ser Leu Gly Phe Ala Leu 
    450                 455                 460                 


Asn Ser Gly Arg Ile Ala Gly Ser Ser Ala Val Glu Tyr Ile Lys Ala 
465                 470                 475                 480 


Asn Leu Met Glu Glu Gln 
                485     


<210>  60
<211>  1230
<212>  DNA
<213>  Clostridium ragsdalei

<400>  60
atgaacaatt taaaagaaga agcattaaag tttcataaag aacatgaagg taaaatagaa       60

cttaaaagta aagtagctgt taaaacaaga gaggatctgg gtttagcata tactccaggt      120

gttgctgaac catgtcttga aatcaacaaa aactataatg ccctatacga ttatacttct      180

aagggaaatt atgtagcagt agtaactaac ggcagtgcag ttttgggact tggaaatata      240

ggtgctgcag ctggcttgcc tgtaatggaa ggtaaatcta ttctatttaa gacttttgca      300

ggagtagacg cttttcctat ttgtgttgac agcaaagatc ctgacaagat tgtagaaaca      360

gtaaaattaa tagaatccac atttggagga ataaacctag aagatataaa agcccctgaa      420

tgctttgaaa tagaagataa attaaaaaag gtctgcaata taccagtttt tcatgacgac      480

caacacggaa cagcagtagt aactttagct gctatgataa acgcgcttaa aatagtaaac      540

aaaaaatttg aagacttaaa agtaataata aatggtgcag gagctgcagg tacagcaatc      600

gcaaagctgc ttgtaagtag aggagttaaa aacattattg tatgcgatag aaaaggtgct      660

atatcaaaag atagagaaaa tttaagtgct gcaaaaaaag acctggcaga aattacaaat      720

cctagcatgg taaaaggtgc gcttaaagat gtactaaaag aagctgatgt atttataggt      780

gtatctgctc ctggagtaat tactcctgaa atgataaaaa caatggataa agatcccctc      840

atttttgcta tggccaatcc taaacctgaa atctaccctg atgaagcaaa agctgcaggt      900

gccagagtag ttggtacagg aagatcggat tttccaaatc aaataaataa tgttcttgca      960

ttccctggaa tatttagagg agcacttgat gtaagagcat caaaaataaa cgaagaaatg     1020

aaaatagctg ctgcatgtgc tatagcagat ataataactg aaaaagaact taatgaagat     1080

tatgttatac cagatgcttt cgactcaaga atagcaccaa aggtagctta ctatgtagca     1140

aaagctgcca tagaaagtgg agttgcaaga agaactgaca tcactcctga aatggtagaa     1200

gaacatacta aaaagcttgt acaagcataa                                      1230


<210>  61
<211>  409
<212>  PRT
<213>  Clostridium ragsdalei

<400>  61

Met Asn Asn Leu Lys Glu Glu Ala Leu Lys Phe His Lys Glu His Glu 
1               5                   10                  15      


Gly Lys Ile Glu Leu Lys Ser Lys Val Ala Val Lys Thr Arg Glu Asp 
            20                  25                  30          


Leu Gly Leu Ala Tyr Thr Pro Gly Val Ala Glu Pro Cys Leu Glu Ile 
        35                  40                  45              


Asn Lys Asn Tyr Asn Ala Leu Tyr Asp Tyr Thr Ser Lys Gly Asn Tyr 
    50                  55                  60                  


Val Ala Val Val Thr Asn Gly Ser Ala Val Leu Gly Leu Gly Asn Ile 
65                  70                  75                  80  


Gly Ala Ala Ala Gly Leu Pro Val Met Glu Gly Lys Ser Ile Leu Phe 
                85                  90                  95      


Lys Thr Phe Ala Gly Val Asp Ala Phe Pro Ile Cys Val Asp Ser Lys 
            100                 105                 110         


Asp Pro Asp Lys Ile Val Glu Thr Val Lys Leu Ile Glu Ser Thr Phe 
        115                 120                 125             


Gly Gly Ile Asn Leu Glu Asp Ile Lys Ala Pro Glu Cys Phe Glu Ile 
    130                 135                 140                 


Glu Asp Lys Leu Lys Lys Val Cys Asn Ile Pro Val Phe His Asp Asp 
145                 150                 155                 160 


Gln His Gly Thr Ala Val Val Thr Leu Ala Ala Met Ile Asn Ala Leu 
                165                 170                 175     


Lys Ile Val Asn Lys Lys Phe Glu Asp Leu Lys Val Ile Ile Asn Gly 
            180                 185                 190         


Ala Gly Ala Ala Gly Thr Ala Ile Ala Lys Leu Leu Val Ser Arg Gly 
        195                 200                 205             


Val Lys Asn Ile Ile Val Cys Asp Arg Lys Gly Ala Ile Ser Lys Asp 
    210                 215                 220                 


Arg Glu Asn Leu Ser Ala Ala Lys Lys Asp Leu Ala Glu Ile Thr Asn 
225                 230                 235                 240 


Pro Ser Met Val Lys Gly Ala Leu Lys Asp Val Leu Lys Glu Ala Asp 
                245                 250                 255     


Val Phe Ile Gly Val Ser Ala Pro Gly Val Ile Thr Pro Glu Met Ile 
            260                 265                 270         


Lys Thr Met Asp Lys Asp Pro Leu Ile Phe Ala Met Ala Asn Pro Lys 
        275                 280                 285             


Pro Glu Ile Tyr Pro Asp Glu Ala Lys Ala Ala Gly Ala Arg Val Val 
    290                 295                 300                 


Gly Thr Gly Arg Ser Asp Phe Pro Asn Gln Ile Asn Asn Val Leu Ala 
305                 310                 315                 320 


Phe Pro Gly Ile Phe Arg Gly Ala Leu Asp Val Arg Ala Ser Lys Ile 
                325                 330                 335     


Asn Glu Glu Met Lys Ile Ala Ala Ala Cys Ala Ile Ala Asp Ile Ile 
            340                 345                 350         


Thr Glu Lys Glu Leu Asn Glu Asp Tyr Val Ile Pro Asp Ala Phe Asp 
        355                 360                 365             


Ser Arg Ile Ala Pro Lys Val Ala Tyr Tyr Val Ala Lys Ala Ala Ile 
    370                 375                 380                 


Glu Ser Gly Val Ala Arg Arg Thr Asp Ile Thr Pro Glu Met Val Glu 
385                 390                 395                 400 


Glu His Thr Lys Lys Leu Val Gln Ala 
                405                 


<210>  62
<211>  1098
<212>  DNA
<213>  Clostridium ragsdalei

<400>  62
atgtcataca ccaaggttaa gtatgaagat ataaaaaagt tgtgtaattt ggtttttgag       60

aaatttggat tcaaccagga agatagtaaa accataacta gcgttttgct tttatcagat      120

ctatatggaa ttgaatcaca tggtatccaa aggctagtca aatactacag tgaaataaaa      180

agtggcctta taaatatcaa ttctaaaatg aaaatagtaa aggaaacacc tgtatctgca      240

acaatagatg gcatgggagg tatgggacaa ctaattggta aaaaagccat gaatctggca      300

attaaaaaag ctaaaacttc aggaatgggt atggtagtag ttagaaattc aaatcactat      360

ggtattgcag gctactatgc caaaatggct gaggaggaag gacttcttgg aatttcaatg      420

accaattctc cagctgtaat ggtaccaacc tttggaaaag atgctatgct tggtacaaat      480

cctattgcca tatcttttcc agctaaaccc tacccatttt taatggatat ggctactagc      540

gtagttacca gaggaaaaat tgaagtttat aacaaaaggc atgaacctct tccacttggt      600

ctcgctttaa atagtgacgg tgaagatact acagatcccc tagatgtact tcttaatgta      660

cgaaaaaatt ctggaggagg cctgcttcct cttggaggat caaaagaatc aactggagga      720

cataaaggtt atggatttgc acttgcagtt gaaatgttta cagcaatttt gtctggagga      780

tttactgcaa ataaagttag cttagataga gaaaatggat ctggaacatg tcattatttc      840

tttgcagtgg attatggtat atttggggat aaacaatcca ttgaagagaa cttttccagt      900

tacctaaatg aacttagaaa ttcaaaaaaa gcaaaaggcg ccacaagaat atatactcat      960

ggtgagaaag aagtagaatc ctataaggat aaaatggaaa atggaattcc agtaaatgag     1020

actactctta aagaaatata cgacatatgt gactacttta atataaaagc tagtgattat     1080

gtaactaaag taatataa                                                   1098


<210>  63
<211>  365
<212>  PRT
<213>  Clostridium ragsdalei

<400>  63

Met Ser Tyr Thr Lys Val Lys Tyr Glu Asp Ile Lys Lys Leu Cys Asn 
1               5                   10                  15      


Leu Val Phe Glu Lys Phe Gly Phe Asn Gln Glu Asp Ser Lys Thr Ile 
            20                  25                  30          


Thr Ser Val Leu Leu Leu Ser Asp Leu Tyr Gly Ile Glu Ser His Gly 
        35                  40                  45              


Ile Gln Arg Leu Val Lys Tyr Tyr Ser Glu Ile Lys Ser Gly Leu Ile 
    50                  55                  60                  


Asn Ile Asn Ser Lys Met Lys Ile Val Lys Glu Thr Pro Val Ser Ala 
65                  70                  75                  80  


Thr Ile Asp Gly Met Gly Gly Met Gly Gln Leu Ile Gly Lys Lys Ala 
                85                  90                  95      


Met Asn Leu Ala Ile Lys Lys Ala Lys Thr Ser Gly Met Gly Met Val 
            100                 105                 110         


Val Val Arg Asn Ser Asn His Tyr Gly Ile Ala Gly Tyr Tyr Ala Lys 
        115                 120                 125             


Met Ala Glu Glu Glu Gly Leu Leu Gly Ile Ser Met Thr Asn Ser Pro 
    130                 135                 140                 


Ala Val Met Val Pro Thr Phe Gly Lys Asp Ala Met Leu Gly Thr Asn 
145                 150                 155                 160 


Pro Ile Ala Ile Ser Phe Pro Ala Lys Pro Tyr Pro Phe Leu Met Asp 
                165                 170                 175     


Met Ala Thr Ser Val Val Thr Arg Gly Lys Ile Glu Val Tyr Asn Lys 
            180                 185                 190         


Arg His Glu Pro Leu Pro Leu Gly Leu Ala Leu Asn Ser Asp Gly Glu 
        195                 200                 205             


Asp Thr Thr Asp Pro Leu Asp Val Leu Leu Asn Val Arg Lys Asn Ser 
    210                 215                 220                 


Gly Gly Gly Leu Leu Pro Leu Gly Gly Ser Lys Glu Ser Thr Gly Gly 
225                 230                 235                 240 


His Lys Gly Tyr Gly Phe Ala Leu Ala Val Glu Met Phe Thr Ala Ile 
                245                 250                 255     


Leu Ser Gly Gly Phe Thr Ala Asn Lys Val Ser Leu Asp Arg Glu Asn 
            260                 265                 270         


Gly Ser Gly Thr Cys His Tyr Phe Phe Ala Val Asp Tyr Gly Ile Phe 
        275                 280                 285             


Gly Asp Lys Gln Ser Ile Glu Glu Asn Phe Ser Ser Tyr Leu Asn Glu 
    290                 295                 300                 


Leu Arg Asn Ser Lys Lys Ala Lys Gly Ala Thr Arg Ile Tyr Thr His 
305                 310                 315                 320 


Gly Glu Lys Glu Val Glu Ser Tyr Lys Asp Lys Met Glu Asn Gly Ile 
                325                 330                 335     


Pro Val Asn Glu Thr Thr Leu Lys Glu Ile Tyr Asp Ile Cys Asp Tyr 
            340                 345                 350         


Phe Asn Ile Lys Ala Ser Asp Tyr Val Thr Lys Val Ile 
        355                 360                 365 


<210>  64
<211>  2634
<212>  DNA
<213>  Clostridium ragsdalei

<400>  64
atgaatggta agaagtacgt ttatcttttc aatgaaggaa atgctggcat gagaaattta       60

cttggaggca agggagctaa tcttgcagaa atgaccaatc ttggcatacc cgttcctggt      120

ggatttacta tatccacaga ggcatgtacc aaatattatg aagatggtaa atccatatca      180

cagcaagtta tagatcaaat ttatgatgca cttaaaaatg tggaagagac aacaggaaaa      240

aagtttggaa gtatagagaa tccactgtta gtttcagtaa ggtcaggagc cagagtttct      300

atgccaggaa tgatggatac tatattaaat ttgggattaa atgatgatac tgtaatagga      360

cttaaaaagc taacaggaaa tgaaagattt gcgtatgatt cttatagaag atttattcaa      420

atgttttcag atgtagttat gggaattgaa aagagagaat ttgaagatgt gctggatgac      480

gtaaaaaatg ctaaaggagt aaaatacgat acagatttag atgaatccga tttaaaggat      540

ataatccaga aatttaaaga tatttacaaa aaagaagtaa aggaagactt tcctcaagat      600

cctaaggaac agttaattca gtcagttact gcagtgttta gatcttggga aaaccctaga      660

gcaataattt acagaaggtt aaacgatata tcaggtgatt ggggaactgc agtaaatgtt      720

caatcaatgg tatttggaaa tatgggagag acttcaggaa caggagttgc atttactaga      780

aacccatcta caggggaaaa gtccatattt ggtgaatatc tcataaacgc tcaaggagag      840

gatgtagttg cagggataag aacacctcaa cctataacaa agctaaaaga agaccttcca      900

aaatgttatt ctcaatttat gagtatagcg aataaactcg aaaatcatta taaagatatg      960

caggatatgg agtttactat agagcagggg aaattatatt tccttcagac gagaaacggt     1020

aagagaacag ctcaggctgc acttagaata gcagtaaata tggtagatga aggtctcatc     1080

actaaagaag aggccatact taaagttgag cctaaacagc ttgacacact attgcatcca     1140

aactttgaca gtgatgaatt gaaacgggca gctgtaatag caaatggact tcctgcatca     1200

ccaggagcag cttgtggtaa gatatatttt acagcagatg atgctaagaa acatcatgat     1260

caaggtgaaa aggtaatact tgtaaggtta gagacttccc cagaagatat agaaggaatg     1320

gcagcttctg aaggaatact tacagttaga ggaggtatga catctcatgc agctgttgta     1380

gcaagaggta tgggaacatg ctgtgtagct ggatgtggtg atcttatagt aagtgaaaag     1440

gaaaagcttt tcaaaagatt agataaggtt tataaagaag gagattacat atctttagat     1500

ggaagtactg gaaatgttta tggagagcct ataaagactg tagcaccaga aatatcggga     1560

gattttggaa tcttcatggg atgggctgat aatataagaa aattaggagt tagaaccaat     1620

gcagatacac caagagatgc aaaccaggct attagttttg gtgccgaagg aataggactt     1680

tgtagaacag agcatatgtt cttcgatgaa gatagaatac cagaaatgag agaaatgata     1740

gtttcaaaaa cggaagagca gagaagaaaa gctttagata aattgctacc aagacaaaag     1800

aaagatttta ttggaatata tgaggcaatg gaaggaaaac ctgtcacaat tagatttttg     1860

gatccaccac ttcatgaatt cttacctact gaaactgagg atatagaggc tttagcaaag     1920

gaagtgggag taagttttca agaattgaaa gatactatag attctctaca tgaatttaat     1980

cctatgatgg gacatagagg atgcaggctt actgtttcat atccagaaat agctgaaatg     2040

caaacaaggg ctattataga agcagctata gatgttaaga agagaaaagg gtatgatata     2100

gttccagaaa ttatgatacc tcttgtagga gaagtaaaag aattaaaata tgttaaagat     2160

gtagttgtaa aggtagcaga tgaaataata caaaaagagg gagtcaattt aaaatatgaa     2220

gtaggaacta tgatagaaat tccaagagcg gctattacag ctgatgaaat agcaaaagaa     2280

gctgagttct tctcatttgg aactaatgat ttaactcaaa tgacttttgg attttcaaga     2340

gatgatgcag gtaaattttt aaatgattat tatgataaaa aagtatatga gtttgatcca     2400

ttccaaaagt tagatcagat tggagtagga aaacttgtag agactgctgt aaaattaggt     2460

aaaaagacta gacctgacat tcatcttgga atatgtggag aacatggagg ggatccatct     2520

tctgtagaat ttttccacaa tgtaggactt gactatgtat cttgttcacc atttagggta     2580

cctgtggcaa gacttgctgc agctcaagct caaataaaga atccaagaaa gtaa           2634


<210>  65
<211>  877
<212>  PRT
<213>  Clostridium ragsdalei

<400>  65

Met Asn Gly Lys Lys Tyr Val Tyr Leu Phe Asn Glu Gly Asn Ala Gly 
1               5                   10                  15      


Met Arg Asn Leu Leu Gly Gly Lys Gly Ala Asn Leu Ala Glu Met Thr 
            20                  25                  30          


Asn Leu Gly Ile Pro Val Pro Gly Gly Phe Thr Ile Ser Thr Glu Ala 
        35                  40                  45              


Cys Thr Lys Tyr Tyr Glu Asp Gly Lys Ser Ile Ser Gln Gln Val Ile 
    50                  55                  60                  


Asp Gln Ile Tyr Asp Ala Leu Lys Asn Val Glu Glu Thr Thr Gly Lys 
65                  70                  75                  80  


Lys Phe Gly Ser Ile Glu Asn Pro Leu Leu Val Ser Val Arg Ser Gly 
                85                  90                  95      


Ala Arg Val Ser Met Pro Gly Met Met Asp Thr Ile Leu Asn Leu Gly 
            100                 105                 110         


Leu Asn Asp Asp Thr Val Ile Gly Leu Lys Lys Leu Thr Gly Asn Glu 
        115                 120                 125             


Arg Phe Ala Tyr Asp Ser Tyr Arg Arg Phe Ile Gln Met Phe Ser Asp 
    130                 135                 140                 


Val Val Met Gly Ile Glu Lys Arg Glu Phe Glu Asp Val Leu Asp Asp 
145                 150                 155                 160 


Val Lys Asn Ala Lys Gly Val Lys Tyr Asp Thr Asp Leu Asp Glu Ser 
                165                 170                 175     


Asp Leu Lys Asp Ile Ile Gln Lys Phe Lys Asp Ile Tyr Lys Lys Glu 
            180                 185                 190         


Val Lys Glu Asp Phe Pro Gln Asp Pro Lys Glu Gln Leu Ile Gln Ser 
        195                 200                 205             


Val Thr Ala Val Phe Arg Ser Trp Glu Asn Pro Arg Ala Ile Ile Tyr 
    210                 215                 220                 


Arg Arg Leu Asn Asp Ile Ser Gly Asp Trp Gly Thr Ala Val Asn Val 
225                 230                 235                 240 


Gln Ser Met Val Phe Gly Asn Met Gly Glu Thr Ser Gly Thr Gly Val 
                245                 250                 255     


Ala Phe Thr Arg Asn Pro Ser Thr Gly Glu Lys Ser Ile Phe Gly Glu 
            260                 265                 270         


Tyr Leu Ile Asn Ala Gln Gly Glu Asp Val Val Ala Gly Ile Arg Thr 
        275                 280                 285             


Pro Gln Pro Ile Thr Lys Leu Lys Glu Asp Leu Pro Lys Cys Tyr Ser 
    290                 295                 300                 


Gln Phe Met Ser Ile Ala Asn Lys Leu Glu Asn His Tyr Lys Asp Met 
305                 310                 315                 320 


Gln Asp Met Glu Phe Thr Ile Glu Gln Gly Lys Leu Tyr Phe Leu Gln 
                325                 330                 335     


Thr Arg Asn Gly Lys Arg Thr Ala Gln Ala Ala Leu Arg Ile Ala Val 
            340                 345                 350         


Asn Met Val Asp Glu Gly Leu Ile Thr Lys Glu Glu Ala Ile Leu Lys 
        355                 360                 365             


Val Glu Pro Lys Gln Leu Asp Thr Leu Leu His Pro Asn Phe Asp Ser 
    370                 375                 380                 


Asp Glu Leu Lys Arg Ala Ala Val Ile Ala Asn Gly Leu Pro Ala Ser 
385                 390                 395                 400 


Pro Gly Ala Ala Cys Gly Lys Ile Tyr Phe Thr Ala Asp Asp Ala Lys 
                405                 410                 415     


Lys His His Asp Gln Gly Glu Lys Val Ile Leu Val Arg Leu Glu Thr 
            420                 425                 430         


Ser Pro Glu Asp Ile Glu Gly Met Ala Ala Ser Glu Gly Ile Leu Thr 
        435                 440                 445             


Val Arg Gly Gly Met Thr Ser His Ala Ala Val Val Ala Arg Gly Met 
    450                 455                 460                 


Gly Thr Cys Cys Val Ala Gly Cys Gly Asp Leu Ile Val Ser Glu Lys 
465                 470                 475                 480 


Glu Lys Leu Phe Lys Arg Leu Asp Lys Val Tyr Lys Glu Gly Asp Tyr 
                485                 490                 495     


Ile Ser Leu Asp Gly Ser Thr Gly Asn Val Tyr Gly Glu Pro Ile Lys 
            500                 505                 510         


Thr Val Ala Pro Glu Ile Ser Gly Asp Phe Gly Ile Phe Met Gly Trp 
        515                 520                 525             


Ala Asp Asn Ile Arg Lys Leu Gly Val Arg Thr Asn Ala Asp Thr Pro 
    530                 535                 540                 


Arg Asp Ala Asn Gln Ala Ile Ser Phe Gly Ala Glu Gly Ile Gly Leu 
545                 550                 555                 560 


Cys Arg Thr Glu His Met Phe Phe Asp Glu Asp Arg Ile Pro Glu Met 
                565                 570                 575     


Arg Glu Met Ile Val Ser Lys Thr Glu Glu Gln Arg Arg Lys Ala Leu 
            580                 585                 590         


Asp Lys Leu Leu Pro Arg Gln Lys Lys Asp Phe Ile Gly Ile Tyr Glu 
        595                 600                 605             


Ala Met Glu Gly Lys Pro Val Thr Ile Arg Phe Leu Asp Pro Pro Leu 
    610                 615                 620                 


His Glu Phe Leu Pro Thr Glu Thr Glu Asp Ile Glu Ala Leu Ala Lys 
625                 630                 635                 640 


Glu Val Gly Val Ser Phe Gln Glu Leu Lys Asp Thr Ile Asp Ser Leu 
                645                 650                 655     


His Glu Phe Asn Pro Met Met Gly His Arg Gly Cys Arg Leu Thr Val 
            660                 665                 670         


Ser Tyr Pro Glu Ile Ala Glu Met Gln Thr Arg Ala Ile Ile Glu Ala 
        675                 680                 685             


Ala Ile Asp Val Lys Lys Arg Lys Gly Tyr Asp Ile Val Pro Glu Ile 
    690                 695                 700                 


Met Ile Pro Leu Val Gly Glu Val Lys Glu Leu Lys Tyr Val Lys Asp 
705                 710                 715                 720 


Val Val Val Lys Val Ala Asp Glu Ile Ile Gln Lys Glu Gly Val Asn 
                725                 730                 735     


Leu Lys Tyr Glu Val Gly Thr Met Ile Glu Ile Pro Arg Ala Ala Ile 
            740                 745                 750         


Thr Ala Asp Glu Ile Ala Lys Glu Ala Glu Phe Phe Ser Phe Gly Thr 
        755                 760                 765             


Asn Asp Leu Thr Gln Met Thr Phe Gly Phe Ser Arg Asp Asp Ala Gly 
    770                 775                 780                 


Lys Phe Leu Asn Asp Tyr Tyr Asp Lys Lys Val Tyr Glu Phe Asp Pro 
785                 790                 795                 800 


Phe Gln Lys Leu Asp Gln Ile Gly Val Gly Lys Leu Val Glu Thr Ala 
                805                 810                 815     


Val Lys Leu Gly Lys Lys Thr Arg Pro Asp Ile His Leu Gly Ile Cys 
            820                 825                 830         


Gly Glu His Gly Gly Asp Pro Ser Ser Val Glu Phe Phe His Asn Val 
        835                 840                 845             


Gly Leu Asp Tyr Val Ser Cys Ser Pro Phe Arg Val Pro Val Ala Arg 
    850                 855                 860                 


Leu Ala Ala Ala Gln Ala Gln Ile Lys Asn Pro Arg Lys 
865                 870                 875         


<210>  66
<211>  3447
<212>  DNA
<213>  Clostridium ragsdalei

<400>  66
atggcgtact tgttaaagag gtttaagagg gttcttgtag cgaatagagg agaaatagcc       60

ataagaatat tcagagcatg taaagaattg ggaataacta ctgtagcagt atattcaaat      120

gaggataaga gatctctttt cagaactaaa gctgatgaat cctatatgat agggaaaaat      180

aagggacctg tagaagcata tttagatatt gatgaaataa tagatatagc tttgaagaaa      240

aatgtagatg caatacatcc gggttatgga tttctatcag aaaatcctga attggcaaaa      300

aagtgtaaag aagcaggtat tgaatttata ggacctacat cagatatgat ggagatgctt      360

ggtgataaga taaaatctaa gattgttgca caaaaggctg gggttccaac aataccagga      420

gttcaagagg ctataaagac agaagaagaa gctttaaaat ttgctaagtt ctgtggatat      480

cctgtcatga ttaaagcagc tgatggcggt ggcggcagag gaatgagaat agtaagggaa      540

gaaaaagatc tcgtagaatc ctacaacagt gctaaaaacg aatccagaaa agcttttggt      600

tcagaaaaaa tatatattga aaaatatatt gaaagtccaa aacacataga ggtgcaggta      660

ctcggagata agtacggcaa tattgtccat ctgtatgaaa gagattgttc tatacagagg      720

agacatcaaa aggtgataga atttacacca tctttagccc tctcagaaga aaaaagacaa      780

caaatatgtg aagatgcttt aaaaattgca agaactgtag gatatacaag tgcaggtacc      840

ttggagtttt tggttgataa aaacggaaat cactatttca tagagatgaa tactagaatt      900

caggtagaac atactgtaac tgaaatggtt acaggaatag atatagttca agatcaaata      960

cttattgcag aagggcattc acttgattct aaggaaatag gaataaaatc tcaagatgat     1020

atagagttaa aaggatatgc aatacaatgc agaattacga cagaagatcc tttaaataat     1080

tttgcaccag atacaggaag aatagatatg tatagaactg gttctggatt tggtataaga     1140

cttgatggag ggaatggatt tacaggcgca gtaataagtc ctcattatga tagtttgcta     1200

gtaaaaactg tatcttggtc aagaactttt gaagatgcca taagaaaggc aataaggtct     1260

ataaatgaga ctgttatatc aggagtaaag acaaatgcag actttataat aaaagtgtta     1320

agtcatgaaa agtttataaa aggtgaatgt gatactaatt ttattgaaga taatccagat     1380

ttatttgata taaaaccaaa actagataaa gagatgagtg tacttaaatt tataggaaat     1440

aaagtagtaa atgagactcg tggaaagaag aagaaattta atatacctat tgtaccaaaa     1500

gtagaagaaa atattaaatt gagtggaacg aagcagatac ttgataccaa aggagcagat     1560

ggattagttg attggataaa atcacaagat aagcttctta ttacagatac tactatgaga     1620

gatgcccatc agtcgcttat ggcaactagg gtgagaacta gagatttgct taagatagca     1680

aaagcacaat cagtattgac aaacgatctt ttctccatgg aaatgtgggg aggagcaact     1740

tttgatgtag cttatagatt tttaaatgaa tctccttggg aaagactaga aaaacttaga     1800

gaaaaggttc ctaatatact attccagatg ctcataagag gagctaatgc agtaggatat     1860

aagaactatc ctgataatgt tattagagaa tttataaaac aatctgcagc ttcaggtatt     1920

gatgtattta gagtatttga tgctttaaac tggcttaaag gaatggaagt ttctatagat     1980

cagacattaa aagaaggaaa aatagctgaa gcatgtatgt gctatacagg agatgtatta     2040

gatgacaagg aagataaata tacacttcag tactatgtaa acttagctaa agaaatagag     2100

aaaactggag cacagattct tggaataaag gatatgtctg ccctattaaa gccatattct     2160

gcttataaac ttgtaaaagc acttaagaat gaggtatcta ttccaataca tcttcatact     2220

catgatacta caggtaatgg tgtggcaaca gtactcatgg ctgccgatgc aggacttgat     2280

atagctgata ctgcattcaa tagtatgtct gggcttacta gccagccagc tttgaattca     2340

atagcagcag cacttaaaaa tacacctaga gatactaagt tagatgcaga taatcttcaa     2400

aaaatatcta actattggga agatgtaagg cctatataca gtcagtttga gtcaggactt     2460

aagtcgagta ctgcagaaat atacaagtat gagataccag gaggtcaata ttcaaactta     2520

aaacctcagg ttgaaagttt tgggttgggg gatcgttttg aagatgtaaa ggaaatgtat     2580

aagagagtta ataaaatgct tggcaacata attaaagtaa ctccttcttc aaaaatggta     2640

ggagacctgg ctatattcat gatacaaaat gatttagatg aaaagaatat ttatgaaaaa     2700

ggtaagagct taactttccc agattctaca atttcttact ttaagggaat gatgggtcag     2760

cctatgggag gattcccaaa ggaacttcaa aaagtagttt taaagggaga agaacctttt     2820

acggtaagac caggagaact tttaccacca gaagattttg ccaaaataaa agagtattta     2880

actaaaaaat ataagagaga atttaataat aaagaactta taagctatgc tatgtatcct     2940

gatgtatatg aaggttatct taaattctta agtgaatatg gtgatcttag cagaatggaa     3000

agtgaaacat tcttctatgg acttgcggaa ggagaacttt gtgaagttga aataggagaa     3060

ggaaagagtt tatttgtaca gttattagag attacaaaag ttgatgatga aggatacaga     3120

ttcttagtat ttgaggtaaa tggtattaag agagacataa ggataaaaga taacttggct     3180

ttctctggat caggaataaa agaaaattca tgtgttatgg cagatgaaga tgatgaaaaa     3240

gaaataggat caagtatacc tggaaacatt gttaaagtac ttgtaaaacc aggagataaa     3300

gtagaagagg gccagagctt aattgtaata gaagctatga aaatggaaac aaatgtttca     3360

gctgctgaag caggagtaat tgatggagta tttgtaaaag aaggccagag agttaaaact     3420

ggagaacttt taattagatt aaaatag                                         3447


<210>  67
<211>  1148
<212>  PRT
<213>  Clostridium ragsdalei

<400>  67

Met Ala Tyr Leu Leu Lys Arg Phe Lys Arg Val Leu Val Ala Asn Arg 
1               5                   10                  15      


Gly Glu Ile Ala Ile Arg Ile Phe Arg Ala Cys Lys Glu Leu Gly Ile 
            20                  25                  30          


Thr Thr Val Ala Val Tyr Ser Asn Glu Asp Lys Arg Ser Leu Phe Arg 
        35                  40                  45              


Thr Lys Ala Asp Glu Ser Tyr Met Ile Gly Lys Asn Lys Gly Pro Val 
    50                  55                  60                  


Glu Ala Tyr Leu Asp Ile Asp Glu Ile Ile Asp Ile Ala Leu Lys Lys 
65                  70                  75                  80  


Asn Val Asp Ala Ile His Pro Gly Tyr Gly Phe Leu Ser Glu Asn Pro 
                85                  90                  95      


Glu Leu Ala Lys Lys Cys Lys Glu Ala Gly Ile Glu Phe Ile Gly Pro 
            100                 105                 110         


Thr Ser Asp Met Met Glu Met Leu Gly Asp Lys Ile Lys Ser Lys Ile 
        115                 120                 125             


Val Ala Gln Lys Ala Gly Val Pro Thr Ile Pro Gly Val Gln Glu Ala 
    130                 135                 140                 


Ile Lys Thr Glu Glu Glu Ala Leu Lys Phe Ala Lys Phe Cys Gly Tyr 
145                 150                 155                 160 


Pro Val Met Ile Lys Ala Ala Asp Gly Gly Gly Gly Arg Gly Met Arg 
                165                 170                 175     


Ile Val Arg Glu Glu Lys Asp Leu Val Glu Ser Tyr Asn Ser Ala Lys 
            180                 185                 190         


Asn Glu Ser Arg Lys Ala Phe Gly Ser Glu Lys Ile Tyr Ile Glu Lys 
        195                 200                 205             


Tyr Ile Glu Ser Pro Lys His Ile Glu Val Gln Val Leu Gly Asp Lys 
    210                 215                 220                 


Tyr Gly Asn Ile Val His Leu Tyr Glu Arg Asp Cys Ser Ile Gln Arg 
225                 230                 235                 240 


Arg His Gln Lys Val Ile Glu Phe Thr Pro Ser Leu Ala Leu Ser Glu 
                245                 250                 255     


Glu Lys Arg Gln Gln Ile Cys Glu Asp Ala Leu Lys Ile Ala Arg Thr 
            260                 265                 270         


Val Gly Tyr Thr Ser Ala Gly Thr Leu Glu Phe Leu Val Asp Lys Asn 
        275                 280                 285             


Gly Asn His Tyr Phe Ile Glu Met Asn Thr Arg Ile Gln Val Glu His 
    290                 295                 300                 


Thr Val Thr Glu Met Val Thr Gly Ile Asp Ile Val Gln Asp Gln Ile 
305                 310                 315                 320 


Leu Ile Ala Glu Gly His Ser Leu Asp Ser Lys Glu Ile Gly Ile Lys 
                325                 330                 335     


Ser Gln Asp Asp Ile Glu Leu Lys Gly Tyr Ala Ile Gln Cys Arg Ile 
            340                 345                 350         


Thr Thr Glu Asp Pro Leu Asn Asn Phe Ala Pro Asp Thr Gly Arg Ile 
        355                 360                 365             


Asp Met Tyr Arg Thr Gly Ser Gly Phe Gly Ile Arg Leu Asp Gly Gly 
    370                 375                 380                 


Asn Gly Phe Thr Gly Ala Val Ile Ser Pro His Tyr Asp Ser Leu Leu 
385                 390                 395                 400 


Val Lys Thr Val Ser Trp Ser Arg Thr Phe Glu Asp Ala Ile Arg Lys 
                405                 410                 415     


Ala Ile Arg Ser Ile Asn Glu Thr Val Ile Ser Gly Val Lys Thr Asn 
            420                 425                 430         


Ala Asp Phe Ile Ile Lys Val Leu Ser His Glu Lys Phe Ile Lys Gly 
        435                 440                 445             


Glu Cys Asp Thr Asn Phe Ile Glu Asp Asn Pro Asp Leu Phe Asp Ile 
    450                 455                 460                 


Lys Pro Lys Leu Asp Lys Glu Met Ser Val Leu Lys Phe Ile Gly Asn 
465                 470                 475                 480 


Lys Val Val Asn Glu Thr Arg Gly Lys Lys Lys Lys Phe Asn Ile Pro 
                485                 490                 495     


Ile Val Pro Lys Val Glu Glu Asn Ile Lys Leu Ser Gly Thr Lys Gln 
            500                 505                 510         


Ile Leu Asp Thr Lys Gly Ala Asp Gly Leu Val Asp Trp Ile Lys Ser 
        515                 520                 525             


Gln Asp Lys Leu Leu Ile Thr Asp Thr Thr Met Arg Asp Ala His Gln 
    530                 535                 540                 


Ser Leu Met Ala Thr Arg Val Arg Thr Arg Asp Leu Leu Lys Ile Ala 
545                 550                 555                 560 


Lys Ala Gln Ser Val Leu Thr Asn Asp Leu Phe Ser Met Glu Met Trp 
                565                 570                 575     


Gly Gly Ala Thr Phe Asp Val Ala Tyr Arg Phe Leu Asn Glu Ser Pro 
            580                 585                 590         


Trp Glu Arg Leu Glu Lys Leu Arg Glu Lys Val Pro Asn Ile Leu Phe 
        595                 600                 605             


Gln Met Leu Ile Arg Gly Ala Asn Ala Val Gly Tyr Lys Asn Tyr Pro 
    610                 615                 620                 


Asp Asn Val Ile Arg Glu Phe Ile Lys Gln Ser Ala Ala Ser Gly Ile 
625                 630                 635                 640 


Asp Val Phe Arg Val Phe Asp Ala Leu Asn Trp Leu Lys Gly Met Glu 
                645                 650                 655     


Val Ser Ile Asp Gln Thr Leu Lys Glu Gly Lys Ile Ala Glu Ala Cys 
            660                 665                 670         


Met Cys Tyr Thr Gly Asp Val Leu Asp Asp Lys Glu Asp Lys Tyr Thr 
        675                 680                 685             


Leu Gln Tyr Tyr Val Asn Leu Ala Lys Glu Ile Glu Lys Thr Gly Ala 
    690                 695                 700                 


Gln Ile Leu Gly Ile Lys Asp Met Ser Ala Leu Leu Lys Pro Tyr Ser 
705                 710                 715                 720 


Ala Tyr Lys Leu Val Lys Ala Leu Lys Asn Glu Val Ser Ile Pro Ile 
                725                 730                 735     


His Leu His Thr His Asp Thr Thr Gly Asn Gly Val Ala Thr Val Leu 
            740                 745                 750         


Met Ala Ala Asp Ala Gly Leu Asp Ile Ala Asp Thr Ala Phe Asn Ser 
        755                 760                 765             


Met Ser Gly Leu Thr Ser Gln Pro Ala Leu Asn Ser Ile Ala Ala Ala 
    770                 775                 780                 


Leu Lys Asn Thr Pro Arg Asp Thr Lys Leu Asp Ala Asp Asn Leu Gln 
785                 790                 795                 800 


Lys Ile Ser Asn Tyr Trp Glu Asp Val Arg Pro Ile Tyr Ser Gln Phe 
                805                 810                 815     


Glu Ser Gly Leu Lys Ser Ser Thr Ala Glu Ile Tyr Lys Tyr Glu Ile 
            820                 825                 830         


Pro Gly Gly Gln Tyr Ser Asn Leu Lys Pro Gln Val Glu Ser Phe Gly 
        835                 840                 845             


Leu Gly Asp Arg Phe Glu Asp Val Lys Glu Met Tyr Lys Arg Val Asn 
    850                 855                 860                 


Lys Met Leu Gly Asn Ile Ile Lys Val Thr Pro Ser Ser Lys Met Val 
865                 870                 875                 880 


Gly Asp Leu Ala Ile Phe Met Ile Gln Asn Asp Leu Asp Glu Lys Asn 
                885                 890                 895     


Ile Tyr Glu Lys Gly Lys Ser Leu Thr Phe Pro Asp Ser Thr Ile Ser 
            900                 905                 910         


Tyr Phe Lys Gly Met Met Gly Gln Pro Met Gly Gly Phe Pro Lys Glu 
        915                 920                 925             


Leu Gln Lys Val Val Leu Lys Gly Glu Glu Pro Phe Thr Val Arg Pro 
    930                 935                 940                 


Gly Glu Leu Leu Pro Pro Glu Asp Phe Ala Lys Ile Lys Glu Tyr Leu 
945                 950                 955                 960 


Thr Lys Lys Tyr Lys Arg Glu Phe Asn Asn Lys Glu Leu Ile Ser Tyr 
                965                 970                 975     


Ala Met Tyr Pro Asp Val Tyr Glu Gly Tyr Leu Lys Phe Leu Ser Glu 
            980                 985                 990         


Tyr Gly Asp Leu Ser Arg Met Glu  Ser Glu Thr Phe Phe  Tyr Gly Leu 
        995                 1000                 1005             


Ala Glu  Gly Glu Leu Cys Glu  Val Glu Ile Gly Glu  Gly Lys Ser 
    1010                 1015                 1020             


Leu Phe  Val Gln Leu Leu Glu  Ile Thr Lys Val Asp  Asp Glu Gly 
    1025                 1030                 1035             


Tyr Arg  Phe Leu Val Phe Glu  Val Asn Gly Ile Lys  Arg Asp Ile 
    1040                 1045                 1050             


Arg Ile  Lys Asp Asn Leu Ala  Phe Ser Gly Ser Gly  Ile Lys Glu 
    1055                 1060                 1065             


Asn Ser  Cys Val Met Ala Asp  Glu Asp Asp Glu Lys  Glu Ile Gly 
    1070                 1075                 1080             


Ser Ser  Ile Pro Gly Asn Ile  Val Lys Val Leu Val  Lys Pro Gly 
    1085                 1090                 1095             


Asp Lys  Val Glu Glu Gly Gln  Ser Leu Ile Val Ile  Glu Ala Met 
    1100                 1105                 1110             


Lys Met  Glu Thr Asn Val Ser  Ala Ala Glu Ala Gly  Val Ile Asp 
    1115                 1120                 1125             


Gly Val  Phe Val Lys Glu Gly  Gln Arg Val Lys Thr  Gly Glu Leu 
    1130                 1135                 1140             


Leu Ile  Arg Leu Lys 
    1145             


<210>  68
<211>  1506
<212>  DNA
<213>  Clostridium ragsdalei

<400>  68
ttgaatatta ataaatatag aaatatgtat aaaaatttat caccatcgga attaacggaa       60

ttttcaatta aaaggggaga aggattttta tcaaataagg gagctcttat gattaatact      120

ggaaagtata caggaagatc tcctaaagat agatttatag ttaatcaaga aagcattagg      180

aacaaaataa actggggaaa tgtaaatctt tctatagaag aagatatttt taataaaatg      240

tatgataaga ttttaaatta tataagtgat aaagacattt ttgtgtttga tggatttgtt      300

ggagctttaa aaaaatatac ccttcctata agagtaatat gcgaaagagc atcccaggcg      360

ttgtttgcaa atcaattgtt tagaaggcca acggaggagg atttaaagtg ttttactcct      420

gaatttaata ttatatcggt acctggattt aaagctaagg ggaaagagga cggtttaaat      480

tcagatgcct ttattttagt aaattttgat aaaaaaatta tattaatagg gggaaccagt      540

tactcgggag aaataaaaaa atcagtattt tcagtaatga actttttgct tccacaaaaa      600

ggggtcatgc ctatgcactg ttctgctaat ataggacaag acaataaaac ttgcttattt      660

tttgggttgt caggaacagg aaaaaccact ttatcagcag atggtgaaag aagattaatt      720

ggtgatgacg aacatggatg gtctaatgaa ggtgtattta attttgaggg tggatgttat      780

gctaaaacta taaggcttga taaggaaaag gaaagccaga tatacaatgc cataaaattt      840

ggaactgtag ttgaaaatgt agtggcagat gagaataggg tacctgatta taatgatggt      900

aggtatactg aaaatacaag ggcagcatat cctataaatt atatagataa tatagaagaa      960

agcggtgtag gaggaaatcc agagactata atatttttaa ctgcagatgc ttttggtgta     1020

atgccaccta tatcaagact ttctaaagaa gcagcaatgt atcactttat gtctggatat     1080

accagcaaga tagctggaac tgaaagagga ataattgaac ctcaagctac tttttcctct     1140

tgctttggtg aaccttttat gttaatgaat cctgctgtct atgcaaagct gttaggcgaa     1200

agaatagaca agtataacac ccaggtatat ttagtgaata ctggatggct atctggagga     1260

tatggaaatg gagatagaat aaaactttcc tatacaagaa ctatgattag agaagctttg     1320

aaaggaaaat tcaaagatgt tgattttgtg gaacatcctg tgtttaaagt aatgatgcct     1380

acaagatgtc caggtgtacc tgatgaaata ttaaacccta gaaatacatg gcaagataaa     1440

gaagcgtatg atgagacagc gagaaagctg gcaatgaagt ttagtaaaaa ctttgagaag     1500

ttttaa                                                                1506


<210>  69
<211>  501
<212>  PRT
<213>  Clostridium ragsdalei

<400>  69

Met Asn Ile Asn Lys Tyr Arg Asn Met Tyr Lys Asn Leu Ser Pro Ser 
1               5                   10                  15      


Glu Leu Thr Glu Phe Ser Ile Lys Arg Gly Glu Gly Phe Leu Ser Asn 
            20                  25                  30          


Lys Gly Ala Leu Met Ile Asn Thr Gly Lys Tyr Thr Gly Arg Ser Pro 
        35                  40                  45              


Lys Asp Arg Phe Ile Val Asn Gln Glu Ser Ile Arg Asn Lys Ile Asn 
    50                  55                  60                  


Trp Gly Asn Val Asn Leu Ser Ile Glu Glu Asp Ile Phe Asn Lys Met 
65                  70                  75                  80  


Tyr Asp Lys Ile Leu Asn Tyr Ile Ser Asp Lys Asp Ile Phe Val Phe 
                85                  90                  95      


Asp Gly Phe Val Gly Ala Leu Lys Lys Tyr Thr Leu Pro Ile Arg Val 
            100                 105                 110         


Ile Cys Glu Arg Ala Ser Gln Ala Leu Phe Ala Asn Gln Leu Phe Arg 
        115                 120                 125             


Arg Pro Thr Glu Glu Asp Leu Lys Cys Phe Thr Pro Glu Phe Asn Ile 
    130                 135                 140                 


Ile Ser Val Pro Gly Phe Lys Ala Lys Gly Lys Glu Asp Gly Leu Asn 
145                 150                 155                 160 


Ser Asp Ala Phe Ile Leu Val Asn Phe Asp Lys Lys Ile Ile Leu Ile 
                165                 170                 175     


Gly Gly Thr Ser Tyr Ser Gly Glu Ile Lys Lys Ser Val Phe Ser Val 
            180                 185                 190         


Met Asn Phe Leu Leu Pro Gln Lys Gly Val Met Pro Met His Cys Ser 
        195                 200                 205             


Ala Asn Ile Gly Gln Asp Asn Lys Thr Cys Leu Phe Phe Gly Leu Ser 
    210                 215                 220                 


Gly Thr Gly Lys Thr Thr Leu Ser Ala Asp Gly Glu Arg Arg Leu Ile 
225                 230                 235                 240 


Gly Asp Asp Glu His Gly Trp Ser Asn Glu Gly Val Phe Asn Phe Glu 
                245                 250                 255     


Gly Gly Cys Tyr Ala Lys Thr Ile Arg Leu Asp Lys Glu Lys Glu Ser 
            260                 265                 270         


Gln Ile Tyr Asn Ala Ile Lys Phe Gly Thr Val Val Glu Asn Val Val 
        275                 280                 285             


Ala Asp Glu Asn Arg Val Pro Asp Tyr Asn Asp Gly Arg Tyr Thr Glu 
    290                 295                 300                 


Asn Thr Arg Ala Ala Tyr Pro Ile Asn Tyr Ile Asp Asn Ile Glu Glu 
305                 310                 315                 320 


Ser Gly Val Gly Gly Asn Pro Glu Thr Ile Ile Phe Leu Thr Ala Asp 
                325                 330                 335     


Ala Phe Gly Val Met Pro Pro Ile Ser Arg Leu Ser Lys Glu Ala Ala 
            340                 345                 350         


Met Tyr His Phe Met Ser Gly Tyr Thr Ser Lys Ile Ala Gly Thr Glu 
        355                 360                 365             


Arg Gly Ile Ile Glu Pro Gln Ala Thr Phe Ser Ser Cys Phe Gly Glu 
    370                 375                 380                 


Pro Phe Met Leu Met Asn Pro Ala Val Tyr Ala Lys Leu Leu Gly Glu 
385                 390                 395                 400 


Arg Ile Asp Lys Tyr Asn Thr Gln Val Tyr Leu Val Asn Thr Gly Trp 
                405                 410                 415     


Leu Ser Gly Gly Tyr Gly Asn Gly Asp Arg Ile Lys Leu Ser Tyr Thr 
            420                 425                 430         


Arg Thr Met Ile Arg Glu Ala Leu Lys Gly Lys Phe Lys Asp Val Asp 
        435                 440                 445             


Phe Val Glu His Pro Val Phe Lys Val Met Met Pro Thr Arg Cys Pro 
    450                 455                 460                 


Gly Val Pro Asp Glu Ile Leu Asn Pro Arg Asn Thr Trp Gln Asp Lys 
465                 470                 475                 480 


Glu Ala Tyr Asp Glu Thr Ala Arg Lys Leu Ala Met Lys Phe Ser Lys 
                485                 490                 495     


Asn Phe Glu Lys Phe 
            500     


<210>  70
<211>  843
<212>  DNA
<213>  Clostridium ragsdalei

<400>  70
atgagagaag tagatgtatc cactataaca aaagctgtta gaaatctctg tatagatgcc       60

aattattatc tttcggagga tgttaagaaa aagataaaag aatgtgaaga ggacgaaaaa      120

tggcctactg caaaagacat tttaggtaaa atacttgaaa atatagatat atctaaaaat      180

gaagatgtgc ctatgtgtca ggatacagga atggcttgtg tatttgtaac aattggccag      240

gatgttcata tagtaggagg aagtttagaa gacgcaataa ataagggagt aagtcaggga      300

tatgtagaag ggtatttaag aaagtctgta gtctctgatc ctataaatag agttaatact      360

aaggataata ctcctgcagt aatatattat gaaatagttc caggagataa acttaacata      420

aaagtggctc ctaaaggatt tggatcagaa aatatgagcc agataaaaat gcttaaacca      480

gcagatggac ttaagggtgt taaagatttc gtaataaaag tagtaaagga cgcaggacca      540

aatccatgtc ctcctatggt tgtaggagta ggtataggag gaacttttga caaggctgca      600

aatcttgcaa agaaagctct tgtaagacca ttatctgaaa gaaataaaaa taagttttat      660

tcagatttag aaaatgaact tttagacaaa ataaatttcc taggtatagg acctcaagga      720

ctagggggaa agactacagc tcttgcagta aatatagaaa cttatcctac gcatatagca      780

ggattacctg tagccgtaaa tataaattgc catgttacaa gacataagga aatagaattg      840

taa                                                                    843


<210>  71
<211>  280
<212>  PRT
<213>  Clostridium ragsdalei

<400>  71

Met Arg Glu Val Asp Val Ser Thr Ile Thr Lys Ala Val Arg Asn Leu 
1               5                   10                  15      


Cys Ile Asp Ala Asn Tyr Tyr Leu Ser Glu Asp Val Lys Lys Lys Ile 
            20                  25                  30          


Lys Glu Cys Glu Glu Asp Glu Lys Trp Pro Thr Ala Lys Asp Ile Leu 
        35                  40                  45              


Gly Lys Ile Leu Glu Asn Ile Asp Ile Ser Lys Asn Glu Asp Val Pro 
    50                  55                  60                  


Met Cys Gln Asp Thr Gly Met Ala Cys Val Phe Val Thr Ile Gly Gln 
65                  70                  75                  80  


Asp Val His Ile Val Gly Gly Ser Leu Glu Asp Ala Ile Asn Lys Gly 
                85                  90                  95      


Val Ser Gln Gly Tyr Val Glu Gly Tyr Leu Arg Lys Ser Val Val Ser 
            100                 105                 110         


Asp Pro Ile Asn Arg Val Asn Thr Lys Asp Asn Thr Pro Ala Val Ile 
        115                 120                 125             


Tyr Tyr Glu Ile Val Pro Gly Asp Lys Leu Asn Ile Lys Val Ala Pro 
    130                 135                 140                 


Lys Gly Phe Gly Ser Glu Asn Met Ser Gln Ile Lys Met Leu Lys Pro 
145                 150                 155                 160 


Ala Asp Gly Leu Lys Gly Val Lys Asp Phe Val Ile Lys Val Val Lys 
                165                 170                 175     


Asp Ala Gly Pro Asn Pro Cys Pro Pro Met Val Val Gly Val Gly Ile 
            180                 185                 190         


Gly Gly Thr Phe Asp Lys Ala Ala Asn Leu Ala Lys Lys Ala Leu Val 
        195                 200                 205             


Arg Pro Leu Ser Glu Arg Asn Lys Asn Lys Phe Tyr Ser Asp Leu Glu 
    210                 215                 220                 


Asn Glu Leu Leu Asp Lys Ile Asn Phe Leu Gly Ile Gly Pro Gln Gly 
225                 230                 235                 240 


Leu Gly Gly Lys Thr Thr Ala Leu Ala Val Asn Ile Glu Thr Tyr Pro 
                245                 250                 255     


Thr His Ile Ala Gly Leu Pro Val Ala Val Asn Ile Asn Cys His Val 
            260                 265                 270         


Thr Arg His Lys Glu Ile Glu Leu 
        275                 280 


<210>  72
<211>  574
<212>  DNA
<213>  Clostridium ragsdalei

<400>  72
atgtatatgg aaaaaaagat aactactccg ttaacggaag waaaaggtta aaactttaaa       60

agcaggggat agtgttttaa tatcagggac aatatatact gctagagatg ctgctcataa      120

gagattggtc gagttattag atgaaggtaa atctcttcct atagatgtaa aagatgcaat      180

aatatattac gcaggaccaa gtcctgcaaa accaggccat gtaataggtt cagctggacc      240

aacaagtagt tatagaatgg acccatttgc accaagactg cttgatatag ggttaaaagg      300

aatgatagga aaaggccttc gttcaaaaga agttatagaa tccatgaaga aaaatggagc      360

tgtttacttt gctgcaatag gcggggctgc agcacttgta gcaaaatcca taaagaaagc      420

agaagtagta gcttatgaag atttggattc tgaagctata agaaaattag aagtaaaaga      480

tttacctgta attgtagtaa tagattcaga gggcaataat ttatatgaat caggacgaaa      540

agagtacttg gactctgtgg gccagtctaa gtaa                                  574


<210>  73
<211>  190
<212>  PRT
<213>  Clostridium ragsdalei

<400>  73

Met Tyr Met Glu Lys Lys Ile Thr Thr Pro Leu Thr Glu Glu Lys Val 
1               5                   10                  15      


Lys Thr Leu Lys Ala Gly Asp Ser Val Leu Ile Ser Gly Thr Ile Tyr 
            20                  25                  30          


Thr Ala Arg Asp Ala Ala His Lys Arg Leu Val Glu Leu Leu Asp Glu 
        35                  40                  45              


Gly Lys Ser Leu Pro Ile Asp Val Lys Asp Ala Ile Ile Tyr Tyr Ala 
    50                  55                  60                  


Gly Pro Ser Pro Ala Lys Pro Gly His Val Ile Gly Ser Ala Gly Pro 
65                  70                  75                  80  


Thr Ser Ser Tyr Arg Met Asp Pro Phe Ala Pro Arg Leu Leu Asp Ile 
                85                  90                  95      


Gly Leu Lys Gly Met Ile Gly Lys Gly Leu Arg Ser Lys Glu Val Ile 
            100                 105                 110         


Glu Ser Met Lys Lys Asn Gly Ala Val Tyr Phe Ala Ala Ile Gly Gly 
        115                 120                 125             


Ala Ala Ala Leu Val Ala Lys Ser Ile Lys Lys Ala Glu Val Val Ala 
    130                 135                 140                 


Tyr Glu Asp Leu Asp Ser Glu Ala Ile Arg Lys Leu Glu Val Lys Asp 
145                 150                 155                 160 


Leu Pro Val Ile Val Val Ile Asp Ser Glu Gly Asn Asn Leu Tyr Glu 
                165                 170                 175     


Ser Gly Arg Lys Glu Tyr Leu Asp Ser Val Gly Gln Ser Lys 
            180                 185                 190 


<210>  74
<211>  1797
<212>  DNA
<213>  Clostridium ragsdalei

<400>  74
atgcaaatag ataagataat tgatactgac atattagttg ttggaggctc tggagcaggg       60

tcaatggcag ctgtaacagc tgctgaaaaa ggagcaaaag tactgcttgc attaaaagga      120

aagcttggga aaagtggtaa tgctattatg gcaggagcag gattttctat ggatggagaa      180

actgcatatt ataaatatgg actcaaggaa gcagatccta gaaatacgaa ggaaaaatta      240

tttgaacaaa ttgtaaagca gtctttttat ctaagtgatc aaaatatggt tgagcagttt      300

gttaatgatt gtggtgaatg ctgctggaaa cttaaacagt ggattgaaaa agcaggacat      360

aaggttgcat tctttggaga agaaggatat ataacatcag gtaaagctgt tgcagatgga      420

tgccgatatg gagtttctga ggcaggcagc attgatgtta tacaagattt tatggttgca      480

gatgttttga tggaagatgg aagagctgta ggtgcagttg gaatagatat atattcagga      540

gagattattg aaattagatc aaagtcagtt attttagcta ctggcggata tcagccctat      600

tcctttaaat gcactgtttc cgatatgact ggcgatggaa tggctatggc gtaccgtgca      660

ggagtcaagc ttgcagatat ggaattttta ttatatatac cagcagttgc cctttcacca      720

tcagtatata aaggttcaat ttatcctttc ttacattcca gtatgcttat gcccattgtt      780

aaaaatggca aaggagaatc aattttagac aatatacctg aaactttact taaaatggcc      840

aaggaaagtg aaatgggaaa gcttatattt acgtattatt atggagatca aattgcaaaa      900

ggaaaagcaa ctccaaatgg aggagtatat tttgattatt ccaatgtacc ttttgatatt      960

tatgaaaaag ccttaaaaaa atctgagcca ttaatgaaca tgtggtatag aaaaggattc     1020

tatcaaggaa acaacttgga tacttttgtt gaaaatataa gaaagggcat tccatgggaa     1080

gtaggtattg gctcagaata cagcatgggt ggcattgaag tagacgaaaa tatgtacact     1140

ggagtaccag gactttatgc agctggtgag actacaagtg gtgtatttgg agctatgagg     1200

gttgcagacg gacttattga aatgcttgta catggttata gagcagcatt gtccgcttgc     1260

aaatatatac aaaatgtaaa tgagccaagt atgaaaaata ctaatattga tagtataatt     1320

aaagatattt tttcacctct tgaaagaaaa gaagggataa gtcctataaa aatacacaga     1380

aatatagaaa agacagctga tgctggattc aactttagaa gaaatgaaga gggacttaca     1440

aaagctttag atgatatttt aaaaatacac aaatatgaca taagcgcaat gagtactaaa     1500

agtaaaaata gagtttataa ctatgaatgg atagaatcag tacaggttcg aaatctttta     1560

acttgcacag aagcaggtgt aagagctgcc cttatgagaa aagaaagtag gggtacacat     1620

atacgtgatg attatgaatt tgtagataat gataactggc ttttaaggat tatgagttta     1680

aaaagtgaag acggaactat gaaattatca accagaaagc ctaaagtaac aacaatggaa     1740

ctcccaaatg gtaaaaataa gaatattcct gattatatgc tttcaatgtt aaagtaa        1797


<210>  75
<211>  598
<212>  PRT
<213>  Clostridium ragsdalei

<400>  75

Met Gln Ile Asp Lys Ile Ile Asp Thr Asp Ile Leu Val Val Gly Gly 
1               5                   10                  15      


Ser Gly Ala Gly Ser Met Ala Ala Val Thr Ala Ala Glu Lys Gly Ala 
            20                  25                  30          


Lys Val Leu Leu Ala Leu Lys Gly Lys Leu Gly Lys Ser Gly Asn Ala 
        35                  40                  45              


Ile Met Ala Gly Ala Gly Phe Ser Met Asp Gly Glu Thr Ala Tyr Tyr 
    50                  55                  60                  


Lys Tyr Gly Leu Lys Glu Ala Asp Pro Arg Asn Thr Lys Glu Lys Leu 
65                  70                  75                  80  


Phe Glu Gln Ile Val Lys Gln Ser Phe Tyr Leu Ser Asp Gln Asn Met 
                85                  90                  95      


Val Glu Gln Phe Val Asn Asp Cys Gly Glu Cys Cys Trp Lys Leu Lys 
            100                 105                 110         


Gln Trp Ile Glu Lys Ala Gly His Lys Val Ala Phe Phe Gly Glu Glu 
        115                 120                 125             


Gly Tyr Ile Thr Ser Gly Lys Ala Val Ala Asp Gly Cys Arg Tyr Gly 
    130                 135                 140                 


Val Ser Glu Ala Gly Ser Ile Asp Val Ile Gln Asp Phe Met Val Ala 
145                 150                 155                 160 


Asp Val Leu Met Glu Asp Gly Arg Ala Val Gly Ala Val Gly Ile Asp 
                165                 170                 175     


Ile Tyr Ser Gly Glu Ile Ile Glu Ile Arg Ser Lys Ser Val Ile Leu 
            180                 185                 190         


Ala Thr Gly Gly Tyr Gln Pro Tyr Ser Phe Lys Cys Thr Val Ser Asp 
        195                 200                 205             


Met Thr Gly Asp Gly Met Ala Met Ala Tyr Arg Ala Gly Val Lys Leu 
    210                 215                 220                 


Ala Asp Met Glu Phe Leu Leu Tyr Ile Pro Ala Val Ala Leu Ser Pro 
225                 230                 235                 240 


Ser Val Tyr Lys Gly Ser Ile Tyr Pro Phe Leu His Ser Ser Met Leu 
                245                 250                 255     


Met Pro Ile Val Lys Asn Gly Lys Gly Glu Ser Ile Leu Asp Asn Ile 
            260                 265                 270         


Pro Glu Thr Leu Leu Lys Met Ala Lys Glu Ser Glu Met Gly Lys Leu 
        275                 280                 285             


Ile Phe Thr Tyr Tyr Tyr Gly Asp Gln Ile Ala Lys Gly Lys Ala Thr 
    290                 295                 300                 


Pro Asn Gly Gly Val Tyr Phe Asp Tyr Ser Asn Val Pro Phe Asp Ile 
305                 310                 315                 320 


Tyr Glu Lys Ala Leu Lys Lys Ser Glu Pro Leu Met Asn Met Trp Tyr 
                325                 330                 335     


Arg Lys Gly Phe Tyr Gln Gly Asn Asn Leu Asp Thr Phe Val Glu Asn 
            340                 345                 350         


Ile Arg Lys Gly Ile Pro Trp Glu Val Gly Ile Gly Ser Glu Tyr Ser 
        355                 360                 365             


Met Gly Gly Ile Glu Val Asp Glu Asn Met Tyr Thr Gly Val Pro Gly 
    370                 375                 380                 


Leu Tyr Ala Ala Gly Glu Thr Thr Ser Gly Val Phe Gly Ala Met Arg 
385                 390                 395                 400 


Val Ala Asp Gly Leu Ile Glu Met Leu Val His Gly Tyr Arg Ala Ala 
                405                 410                 415     


Leu Ser Ala Cys Lys Tyr Ile Gln Asn Val Asn Glu Pro Ser Met Lys 
            420                 425                 430         


Asn Thr Asn Ile Asp Ser Ile Ile Lys Asp Ile Phe Ser Pro Leu Glu 
        435                 440                 445             


Arg Lys Glu Gly Ile Ser Pro Ile Lys Ile His Arg Asn Ile Glu Lys 
    450                 455                 460                 


Thr Ala Asp Ala Gly Phe Asn Phe Arg Arg Asn Glu Glu Gly Leu Thr 
465                 470                 475                 480 


Lys Ala Leu Asp Asp Ile Leu Lys Ile His Lys Tyr Asp Ile Ser Ala 
                485                 490                 495     


Met Ser Thr Lys Ser Lys Asn Arg Val Tyr Asn Tyr Glu Trp Ile Glu 
            500                 505                 510         


Ser Val Gln Val Arg Asn Leu Leu Thr Cys Thr Glu Ala Gly Val Arg 
        515                 520                 525             


Ala Ala Leu Met Arg Lys Glu Ser Arg Gly Thr His Ile Arg Asp Asp 
    530                 535                 540                 


Tyr Glu Phe Val Asp Asn Asp Asn Trp Leu Leu Arg Ile Met Ser Leu 
545                 550                 555                 560 


Lys Ser Glu Asp Gly Thr Met Lys Leu Ser Thr Arg Lys Pro Lys Val 
                565                 570                 575     


Thr Thr Met Glu Leu Pro Asn Gly Lys Asn Lys Asn Ile Pro Asp Tyr 
            580                 585                 590         


Met Leu Ser Met Leu Lys 
        595             


<210>  76
<211>  912
<212>  DNA
<213>  Clostridium ragsdalei

<400>  76
atgagagagt ttgaaacaga tgttgttgtt gttggaggag gagcatcagg gcttgctgca       60

gcagttactg ctgctgaaaa tggtgcaaaa gtaatggtgc ttgaaaaagc taatactaca      120

ggtggatgtg ctaatatggc aatgggccct ctaggtgttg aaacaagaat gcaaagagaa      180

aggcttatag atatatctgt agatagagca tttaataagt tcatggaata ttctcactgg      240

agatcagatg caagattgat aagaagatat ttagagcagt cagcaggaac tattgaatgg      300

ttagaaaata tgggagtaga attcgcatta ccttcaaaat attttccagc ttcagaagca      360

acttggcata ttgttaaacc taaaactgga aaaccaggac tccgtgcagc tgctactatg      420

attaaaatca tgacagaaag agcagaagaa ttaggcgtta aaatattatt agaaacacct      480

gtaaagagta ttattaaaga tcaaggagag ataattggcg taacagctag cgataaagat      540

ggtgaattag aagtatatgc tggagcagtt atcatcggta caggcggatt tggtgataat      600

ccagatttta ttaagaagta tgttggactt gaatggggaa aagatttgtt ctcatataga      660

attcctggat taactggaga tggaatccag atggcttggg atgctggcgc ttcaaaagat      720

tttatgacta tggaaatggt attctttgct cctaacactg gtggatatgc tcctatagag      780

ttacccttcc gtcaacctaa ccttttagtt aacctggatg gtgaaagatt tataaatgaa      840

gaagttatag aaaatcctgt atttaccgca aatgctattg aaaaacaaaa aagaaagttg      900

catattctat aa                                                          912


<210>  77
<211>  303
<212>  PRT
<213>  Clostridium ragsdalei

<400>  77

Met Arg Glu Phe Glu Thr Asp Val Val Val Val Gly Gly Gly Ala Ser 
1               5                   10                  15      


Gly Leu Ala Ala Ala Val Thr Ala Ala Glu Asn Gly Ala Lys Val Met 
            20                  25                  30          


Val Leu Glu Lys Ala Asn Thr Thr Gly Gly Cys Ala Asn Met Ala Met 
        35                  40                  45              


Gly Pro Leu Gly Val Glu Thr Arg Met Gln Arg Glu Arg Leu Ile Asp 
    50                  55                  60                  


Ile Ser Val Asp Arg Ala Phe Asn Lys Phe Met Glu Tyr Ser His Trp 
65                  70                  75                  80  


Arg Ser Asp Ala Arg Leu Ile Arg Arg Tyr Leu Glu Gln Ser Ala Gly 
                85                  90                  95      


Thr Ile Glu Trp Leu Glu Asn Met Gly Val Glu Phe Ala Leu Pro Ser 
            100                 105                 110         


Lys Tyr Phe Pro Ala Ser Glu Ala Thr Trp His Ile Val Lys Pro Lys 
        115                 120                 125             


Thr Gly Lys Pro Gly Leu Arg Ala Ala Ala Thr Met Ile Lys Ile Met 
    130                 135                 140                 


Thr Glu Arg Ala Glu Glu Leu Gly Val Lys Ile Leu Leu Glu Thr Pro 
145                 150                 155                 160 


Val Lys Ser Ile Ile Lys Asp Gln Gly Glu Ile Ile Gly Val Thr Ala 
                165                 170                 175     


Ser Asp Lys Asp Gly Glu Leu Glu Val Tyr Ala Gly Ala Val Ile Ile 
            180                 185                 190         


Gly Thr Gly Gly Phe Gly Asp Asn Pro Asp Phe Ile Lys Lys Tyr Val 
        195                 200                 205             


Gly Leu Glu Trp Gly Lys Asp Leu Phe Ser Tyr Arg Ile Pro Gly Leu 
    210                 215                 220                 


Thr Gly Asp Gly Ile Gln Met Ala Trp Asp Ala Gly Ala Ser Lys Asp 
225                 230                 235                 240 


Phe Met Thr Met Glu Met Val Phe Phe Ala Pro Asn Thr Gly Gly Tyr 
                245                 250                 255     


Ala Pro Ile Glu Leu Pro Phe Arg Gln Pro Asn Leu Leu Val Asn Leu 
            260                 265                 270         


Asp Gly Glu Arg Phe Ile Asn Glu Glu Val Ile Glu Asn Pro Val Phe 
        275                 280                 285             


Thr Ala Asn Ala Ile Glu Lys Gln Lys Arg Lys Leu His Ile Leu 
    290                 295                 300             


<210>  78
<211>  962
<212>  DNA
<213>  Clostridium ljungdahlii

<400>  78
tttcttcaca ggaaaatata cttcagtaac aagatcttta ggaatggtga cttggtgggg       60

gtcagttaca tatacttcat atggtgggtt tgtaagttta tatccttcat tttctaccca      120

ttccctcaac ttagcatata cagatgttaa ttctgaatat gagcccctta aaacagactt      180

cgcacaaagg actccaggca agtatcttgt tccctttaca atctccttta tcggaatggc      240

aagttctgta tcattgccag aaggattgta ttcagcgctg tgataaatag ttattggctt      300

accaagaaag tcaattacaa aaatatatat aaagaaagca aagctacata tattaaagca      360

tttaaggtaa aactaaaaat attataaaaa tgaaattatt ttttctcata gctaaagtta      420

cataatacga ggaggattta taatgaaaaa agtaatagga attataagta ttgtactatt      480

tgtactcgta gcacttcaat cctgtgctgc aggagtagga aatgcattaa gtaataacaa      540

agaagctagt ggatctgctg gattattttt atctgtatgt atgcttattg ctggaataat      600

agcaataata tcaaaatata gtaaaggtat gactataaca gctatagtat tttatttgtt      660

agcttttgtt gtagggattg ctaatgttgg gcatttttca gatttgcaaa tttggtcaat      720

cattaacttg atatttgctg gactattgat atttcatttg cttaaaaata agcaattata      780

taatagcagt gggaaaaagt agaatcatat attgtaatta tttttaatta tgttggcaaa      840

attgaaattg tcactgaaac acctctaaat gttttaaata catatgttta attattgtga      900

cagattctaa tagtagaaag tagaaatttg ctatgttata atgacataga ggtgaatgta      960

at                                                                     962


<210>  79
<211>  977
<212>  DNA
<213>  Clostridium ljungdahlii

<400>  79
actagacagt gctaataaca atgtctagtg ctttttatct tgctcaattt tttcattgag       60

ttcatttaag taagtccacc tgtccatctt ttcgtctagc tctttttcca gtgaattctt      120

ttcggataag agatcttcaa gaagtgcata atcagatgaa gcagcttcca tttctatttt      180

cttttcagat atagattttt ctagatgttc aattacctca tctattttgt caaactccat      240

ttgttctgca taggtaaatt ttagaggctt ttctttttgc aacttatagt tgtttttagc      300

tgtatttttc ttagagctta ttttttcctc tgatattttt gcagttttgt gaaaatagga      360

atagtttcct gtatattgag tgattttacc gtttccttca aaagaaaata ttttatcaac      420

tgttttgtca aggaagtacc tgtcatgaga tacagctata acagctcctt caaaatcgtt      480

aatataatct tctaggattg taagtgtttc tatatccaga tcatttgttg gttcgtccag      540

caaaagtaca ttagggtaat tcatcaatat ttttagaaga tataatcttc ttcgttctcc      600

tcctgaaagt tttccaaggg gagtccattg aactgaaggt tcaaataaaa aattttcaag      660

tacagcagaa gcacttattt tttcacccga tgaagttgac gcatattctg atgtcccacg      720

tatgtattca attacccttt cgttcatatc catatcagaa attccctgag aatagtatcc      780

tatctttact gtttcaccta tatctatagt gccgctgtcc ggcagaattt tttgaactaa      840

aatattcata agagtggatt taccacttcc attaggtcca ataataccta ttctgtcatt      900

atttagtatg ttataagtga aatttttaat taatgtcttt tcaccaaaac ttttgcttat      960

gttatccagg tttatga                                                     977


<210>  80
<211>  962
<212>  DNA
<213>  Clostridium ragsdalei

<400>  80
tttcttcaca ggaaaatata cttcagtaac aagatcttta ggaatggtga cttggtgggg       60

gtcagttaca tatacttcat atggtgggtt tgtaagttta tatccttcat tttctaccca      120

ttccctcaac ttagcatata cagatgttaa ttctgaatat gagcccctta aaacagactt      180

cgcacaaagg actccaggca agtatcttgt tccctttaca atctccttta tcggaatggc      240

aagttctgta tcattgccag aaggattgta ttcagcgctg tgataaatag ttattggctt      300

accaagaaag tcaattacaa aaatatatat aaagaaagca aagctacata tattaaagca      360

tttaaggtaa aactaaaaat attataaaaa tgaaattatt ttttctcata gctaaagtta      420

cataatacga ggaggattta taatgaaaaa agtaatagga attataagta ttgtactatt      480

tgtactcgta gcacttcaat cctgtgctgc aggagtagga aatgcattaa gtaataacaa      540

agaagctagt ggatctgctg gattattttt atctgtatgt atgcttattg ctggaataat      600

agcaataata tcaaaatata gtaaaggtat gactataaca gctatagtat tttatttgtt      660

agcttttgtt gtagggattg ctaatgttgg gcatttttca gatttgcaaa tttggtcaat      720

cattaacttg atatttgctg gactattgat atttcatttg cttaaaaata agcaattata      780

taatagcagt gggaaaaagt agaatcatat attgtaatta tttttaatta tgttggcaaa      840

attgaaattg tcactgaaac acctctaaat gttttaaata catatgttta attattgtga      900

cagattctaa tagtagaaag tagaaatttg ctatgttata atgacataga ggtgaatgta      960

at                                                                     962


<210>  81
<211>  975
<212>  DNA
<213>  Clostridium ragsdalei

<400>  81
ctagacagtg ttaataacaa tgtctagtgt ttttctcttg ttcaattttt tcattgagtt       60

catttaggta agtccacctg tccatctttt cttctaattc tttttccagt gaattctttt      120

cagataagag atcttcaaga agtgcataat cagatgaagc agcttccatt tctattttct      180

tttcagatat agatttttct agattttcaa ttacctcatc tattttgtca aactccattt      240

gttctgcata ggtaaatttt aaaggctttt ctttttgcaa cctataattg tttttagctg      300

tattgttctt agtggttatt ttttcttgtg gtatttttgc agtttcgtga aaatgggagt      360

agtttcctgt atattgagcg attttaccat ttccttcaaa agaaaatatt ttatcaactg      420

ttttgtcaag gaagtatctg tcatgggata cagctataac agttccttca aaatcattaa      480

tataatcctc taggattgta agtgtttcta tatccagatc atttgttggt tcgtccagta      540

aaagtacatt aggataattc atcagtattt ttagaaggta taatcttctt cgttcccctc      600

ctgaaagttt tccaagggga gtccattgaa ctgaaggttc aaatagaaaa ttttcaagta      660

cagcagaagc acttattttt tcacccgatg aagttgaggc atattctgat gtcccacgta      720

tgtattcaat taccctttcg ttcatatcca tatcagaaat tccctgagaa tagtatccta      780

tttttactgt ttcacctata tctatagtac cgctgtccgg cagaattttt tgagttaaaa      840

tattcataag agtggattta ccacttccat taggtccaat aatacctatt ctatcattat      900

ttagtacgtt ataagtgaaa tttttaatta atgttttttc accaaaactt ttgcttatgt      960

tatccaggtt tatga                                                       975


<210>  82
<211>  353
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  82
aaaaaagctt ataattatcc ttagttaacg atcaggtgcg cccagatagg gtgttaagtc       60

aagtagttta aggtactact ctgtaagata acacagaaaa cagccaacct aaccgaaaag      120

cgaaagctga tacgggaaca gagcacggtt ggaaagcgat gagttaccta aagacaatcg      180

ggtacgactg agtcgcaatg ttaatcagat ataaggtata agttgtgttt actgaacgca      240

agtttctaat ttcgatttta actcgataga ggaaagtgtc tgaaacctct agtacaaaga      300

aaggtaagtt aggctgatcg acttatctgt tatcaccaca tttgtacaat ctg             353


<210>  83
<211>  353
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  83
aaaaaagctt ataattatcc ttagcactcg ttgaggtgcg cccagatagg gtgttaagtc       60

aagtagttta aggtactact ctgtaagata acacagaaaa cagccaacct aaccgaaaag      120

cgaaagctga tacgggaaca gagcacggtt ggaaagcgat gagttaccta aagacaatcg      180

ggtacgactg agtcgcaatg ttaatcagat ataaggtata agttgtgttt actgaacgca      240

agtttctaat ttcgattagt gctcgataga ggaaagtgtc tgaaacctct agtacaaaga      300

aaggtaagtt aggctcaacg acttatctgt tatcaccaca tttgtacaat ctg             353


<210>  84
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  84
ctgcacctaa aaccaaagca gtatt                                             25


<210>  85
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  85
atcctttaag caagagtact gcacc                                             25


<210>  86
<211>  520
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  86
ccggatwgka ysctrwgmgw gmarrcytwa saymtksgmk ycsssrcsag gkagccgtaa       60

gcttggatcc cgggawtmgk rgrwggkwsy ckgtksktgs mtccygggra gagggggatw      120

scctcccgaa agggaratta mtacsgcata ataatcagtt ttcwcatgga gactgattta      180

aaggagtaat ccsctttgag atggacccgc ggcgcattag ctagttggta gggtaacggc      240

ctaccaaggc gacratgcgt agcckacctg agagggtgat cggccmcmtt ggaactgaga      300

gacggtccmg actyctacgg gaggcakcag kggggaatwt tgcacaatgg gcgaaagcct      360

gatgcarcaa csccgcgtga gtgaagaagg ttttcggatt gtaaagctwt gtctttgggg      420

acgataatga cggtwcckwa ggaggaagcc mcsgstaact acgtgyywgc mkcckcggta      480

atacgtyggt ggmgagygtt gtyyggaatm wckwgkykta                            520


<210>  87
<211>  639
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  87
gggrraystr amyscwgycr wrmmymmssm tmssskkmrw ckragmcrgr wtcaarctct       60

gttgtcgacr aattcgamwr awccrggatc aaactctgtt gtcgamsaat kcsgrkwaac      120

swaktyacmr cymcrttkts attcrmkatt actagcaact ccaacttcwt gtaggcgagt      180

ttcagcctgc aatccgaact gggggcagtt tttgaggttt gctccacctt gcggtcttgc      240

ttctctctgt actgcccatt gtarcacgtg kgttgccctg racataaggg gcatgatgat      300

ttwacstcwt ccccaccttc ytccgcgtta accmcggcag tcttgctara rtgctcaact      360

aaatgttakc aactaacamc aggggttgck ctckttgcag gacttaacct aacwtctcac      420

gacacgagct gacracaacc atgcaccacc tgtatycctg ccccgaaggg yttctcttat      480

ctctaarata ttmagggtat gtcmwgtcca ggwawggttc ttcgcgttgc ttcgaattaa      540

accacatgct ccgctgcttg tgcgggcccc cgtcaattcc tttgagtttt aatmttgcga      600

tcgtacttcc caggcggagt acttattgtg tttactgcg                             639


<210>  88
<211>  1028
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  88
ccgatwgwaa ysctsgmrgc asrsytwasa ymtksarkyc sysrcaaggk agccgtaagc       60

ttggatcccg ggaaccgsrg aaggkasccs krgsktgsmt mccgggraga gggggatagc      120

cwcccsaaag ggagawtmmy rssrcataat awtcagtttt cacwtggaga ctgwtttaaa      180

ggaktaatcc gctttgagat ggacccgcgg cgcattagct agttggtagg gtaacggcct      240

accaaggcga cgatgcgtag ccgacctgag agggtgatcg gccacattgg aactgagaga      300

cggyccarac tcctacggga ggcakcagtg gggaatattg cacaatgggc gaaagcctga      360

tgcagcaacg ccgcgtgagt gaagaaggtt ttcggattgt aaagctctgt ctttggggac      420

gataatgacg gtacccaagg aggaagccac ggstaactac gkgccascag ccgcggtaat      480

acgtaggtgg cgagcgttgt ccggaattac tggkcgtaaa gagtgcgtag gcggatattt      540

aagtgasatg tgaaataccc gggcttaacy cgggcactgc wtttyaaact ggatatctar      600

agtgcgggag aggakaatgg aattcctwkt gtagcggrtg aaatgcgtak agattaggaa      660

gaacaccagt ggcgaargcg attctctgga ccrtaactga crctgaggya cgaaagcrtg      720

ggtagcaakm aggattagat accctggkta gwccacrccg taaacratga ktactakktg      780

twggaggtwt caccccttyt ktgccrsmkt aaacacaata aktactccsc cckggraagt      840

ackatygcaa gawttaaaac tcaaaggrwt tgaygggggs cccgcycaag yagcggaagc      900

atgtggkttw wttycaakca mtsckaykaa ccttwcctkg rayttkrwmt wmccmgcaww      960

cytwataawt aaagaakccc ttysgkgymr gggwawmmgg gkkggtgyat gkktkgtygt     1020

ywatmycg                                                              1028


<210>  89
<211>  716
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  89
gggtaygtsa ayswgyyatr mrysyskmtm rwskkmrwck ragmcrgrat caarctctgt       60

tgtckacraa ttcggmkrak ccrggatcaa actctgttgk cgacsaattc sgrkgaaccy      120

rkwymcmrck mcrttstsat ycrckaytac tagcaactcc aacttcatgt aggcgagttt      180

cagcctgcaa tccgaactgg gggcagtttt tgaggtttgc tccmccttgc ggtcttgctt      240

ctctctgtac tgcccattgt ascacgtgtg ttgccctgga cataaggggc atgatgattt      300

gacgtcatcc ccaccttcct ccgcgttaac cgcggcagtc ttgctagagt gctcaactaa      360

atgttagcaa ctaacaacag gggttgcgct cgttgcagga cttaacctaa catctcacga      420

cacgagctga cgacaaccat gcaccacctg tatccctgyc ccraagggyt tctcttatct      480

ctaagatawt cagggtatkt yaagtccagg waaggttctt cscrttgytt csaattaaac      540

cacatgctcc gctgcttgtg cgggcccccg tcaattcctt tgagttttaa tcttgcgatc      600

gtacttccya ggcggagtac twattgtgtt tactgyggca sasaarrggt cgatacctcc      660

tacacctagt actcatcgtt tackgmgtgy actaccaggr watstaatwc tgtttg          716


<210>  90
<211>  556
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  90
ccggatwkaw awsctmsmgg cwrrcytwas aymtksgmky csyrrcaagg kagccgtaag       60

cttggatccc gggawscgkr gswggkagcc skwksktgsm tccysggrrg agggggrwws      120

ccwcccgrrr gggagaytmm yrssgsataa taatcakttt tcwcatggar actgatttaa      180

aggagtaatc csctttgaga tggacccscg gcgcattakc tagkkggtag ggtaacggyc      240

taccaaggcg acrktgcgta gccgacctga ragggtgatc ggscacattg kaactgagag      300

amggtccara ctcytacggg aygyagcart ggggaatatt gmacaatggg cgaaagccmg      360

atgcagcaac gccscgtgag tgaagaaggt tttcggattg twaarytctg tctttgggga      420

mgataatgac kgtacccaag gasyaagccw cggstaacta cgtgccagya kyckcggtaa      480

tamktaggtg gcgagcgttg tccggaatta cygggmgtaa agartgcgta rgcggatatt      540

tarkgakatg tgaaat                                                      556


<210>  91
<211>  478
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  91
gggwayytsa mkcwgycrwr maykyrgwta rcskkmrwck ragmcrgrat caarctctgt       60

tgtckacraa ttcgakwrar ccrggatcaa actctgttgt csacmaattc sgmkraawcm      120

rgwyymmact myrttstsaw ycamtwytac tagcaactcc aacttcwtgt akgcgagttt      180

cagcctgcaa tccraactgg gggcagtttt tgaggtttgc tccmccttgc ggtcttgctt      240

ctcyctgtac tgcccattgt agcmcgtgtg ttgcwctggw mataaggggc atgatgattt      300

gacgtcatcc ccaccttcct ccgcgtkaac cgcggsagtc ttgcyagagw gytcaaytaa      360

atgttrscra cwaacaacag gggttgcgct cgttgcagga cmtaamctaa yatctcayga      420

camgagctga cgamaayyat gcaccaccty tatccctgwc yckaagggct tctyttat        478


<210>  92
<211>  561
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  92
ccgawwrgka aygstmwsmr wgcwrrsktw asaymsksam kycskrrcaa ggkagccgta       60

agcttggatc ccgggawycg krgraggkas ycskkksktr sawmyykggr ararggggat      120

wsccwcccgr awggmarawt amtascrcat aataatcagt tttcmcatgg agactgattt      180

awaggagtaa tccgctttga gatggacccg cggcgcwtta gcwagttggt agggtaacgg      240

cctaccaagg cgacgatgcg takccsacct gasagggtga tcggccacat tggaactgar      300

agacggtcca ractcctacg ggaggyakca gtggggaata ttgcacaatg ggcgaaagcc      360

tgatgcakca acgccgcgtg agtgaagaag gttttcsgat tgyaaagctc tgtctttggg      420

gacgataatg acggwaccca aggaggaarc cacggctrac tacgtgccws csgycgyggt      480

aatacrtagg tggkkagcgt tgtccggaat tyctyggckt aatgagtgcg wargcggatm      540

yttaagtgas atstgaaama c                                                561


<210>  93
<211>  362
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  93
agggwaaysk maakgawgat matrmatgas katarcskka awckragmcr ggatcaarct       60

ctgttgtcga craattcgmk wrakccagga tcaaactctg ttgtcgacma attcsgmwra      120

accwaktcmm crckmcrttc tgatyrkmkw ctactagcaa ctccaacttc atgtaggcga      180

gtttcakcct gsaatccgaa ytgggggyag tttttgaggt tyyctccayc ttgcggtctt      240

gcttctytct gtactgccca ttgtakcacg tgtgttgccc tggacataag gggcatgatg      300

atttgacgtc atccccawct tyctccgmgt waaccgcggc agtyttycta rartgctyaa      360

yt                                                                     362


<210>  94
<211>  1160
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  94
agtggcactg gaaaagaact cttagctcaa tctattcaca attatagtga aagatgtgaa       60

ggcccttttg tagctataaa ttgtagttct atacctagag aacttgtaga aagtgagctt      120

tttggttatg aaaaaggagc ttttacggga gctttaaagc aaggaaagcc tggaaagttt      180

gaattagcag atggaggaac tatttttttg gatgaagtag gagagcttcc tcttgatata      240

cagtcaaagc ttttaagggt tcttgataat aataaaatta caagagttgg aggaacttat      300

gaaaaacagc taaatgtaag gataatagga gctacaaaca gggtgctcaa ggatgaaatt      360

aaaaagaaaa atttcagaag tgacctttat tatagattga gtgtgatgaa tataaaaact      420

gtcccactta gggaaagaaa agaagatata gagcttttaa ttaaatattt tatggaagaa      480

ttgaattcta aaagtttgtg taagaagaaa gtagtggaaa aagcatacat agaaaagatt      540

aaagcttatg attggcctgg aaatgttaga gaacttagaa atgtaataga gagggattac      600

tatttaagtg aggataagat ggcccctttg gattatttag aaaaagaagt ttatgaaaaa      660

aatgtctcct ctgatccagt aaatattagt gtgcttccaa tggatgtttt agaaaaagaa      720

aacattgaaa atgcacttaa aaagtgtaag ggaaatatat taaaagctgc aaaatcttta      780

aatatcagta gatctaccat gtatagaaaa atgaaaaagt atggaataaa aagtgtgtca      840

aaatgaccag aaaagagtaa gattctcaaa ataggacact aagtatgtgt cataatggca      900

catagtgatt ttaaatgtct ttttaacagg tttcttgttt ttggtatggc ttttgcttat      960

aaaatatagt gaatatatta acaggtatat gtaaatttta atattgccat actattataa     1020

aaaaggagag ataattatga aagctgtatt gtggtatgat aaaaaagatg taagagtaga     1080

ggaaattgag gaacctaagg taaaagaaaa tgctgtaaaa attaaagtga aatggtgtgg     1140

tatatgtggt tctgacttgc                                                 1160


<210>  95
<211>  834
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  95
tattgaggag gccaaaaatg agctttaaga aaaatgtata cgatacaatg agggaactaa       60

tatctgtgcc aagcatatct ggtacaaaag aagagtgtgc ggcagcagaa aaaatatatg      120

aaaaaatttt ggaaatacct tattttaagg acaatcctga aaatctagga atagagcaaa      180

ttgaagatga tcctttagga agaagctttg tatgggcagt agtaaatgga aatgaaaatt      240

caccaaattc gtttatactt tcaggtcatt tggatgtagt tggagtagaa gaatttggac      300

atttaaaatc tatggctttt gatgtagatg aatgtactaa aagaatctca gaattgaatt      360

tagatgaaga tgctatggag gattttaaat caggagattg gatatttgga aggggaactg      420

cagacatgaa gtttggagtg gccctcaata tggaactttt aagagaattc agtaaagaga      480

gaaactttaa gggaaactta ttacttttag tagttcctgg tgaagagagt aattccgaag      540

gaatgattgc tgcagctcca tttcttctta aattaaagga agagaggaag tacaattact      600

gtggtatgat aatatcagag ccaagtatac ctgaaagagg agaaaaagaa ggcaagagat      660

tatatatagg tagtgtaggt aaaattatgc ctttattttt ttgtgtggga aaagaaactc      720

atgtagggga atctttaaga ggattgaatc caaatttgct agtttcagag ataaacaaat      780

taatggaatg taatccagat ctctcagata gcgtttatga tactgtgact ccac            834


<210>  96
<211>  39
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  96
attcatcctg caggagtggc actggaaaag aactcttag                              39


<210>  97
<211>  37
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  97
gactgcggcc gcgcaagtca gaaccacata taccaca                                37


<210>  98
<211>  35
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  98
atatgctagc tattgaggag gccaaaaatg agctt                                  35


<210>  99
<211>  37
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  99
gactggcgcg ccgtggagtc acagtatcat aaacgct                                37


<210>  100
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  100
aatggcaggg cagataattg taatg                                             25


<210>  101
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  101
aaggcattct gagccagttc tttta                                             25


<210>  102
<211>  1062
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  102
acagttaaaa agcatatcta acagtccttc cactgtacta attcaaggcg aaagcggtac       60

aggtaaagaa cttattgcgc agtccatcca caatgacagc agcagaaaaa ataacagctt      120

tatagcaata aattgcggtg ccatacccaa aaatttaata gaaagtgaat tattcggata      180

tgaagatgga tcattcacag gtgcaaaaca tggagggcgt gcaggaaaat ttgaacttgc      240

aaatggtggt actttatttt tagatgaaat tggggaaatg cctttagata tgcaagtaaa      300

tcttttaaga gttctccaag aaaactgtat tacaagaata ggcgggaaca gatgtgtaaa      360

aatagatata agaatcattg cagctactaa taaaaatttg agggaagaaa tacataaagg      420

aacttttcgc gaagatttat actatagact aaatgtaata cctatatatg taccaccact      480

gcgggaaaga gatatggata ttaaaatact gataaactat tttttaaaga taaaagcttt      540

taaacttaaa aaacctattc caatagtaag acctgatata tatcaaaagc tcttaaatta      600

taattggccc ggaaatgtaa gagaattgga aaattgtatt gaaaatatcg taaatatgaa      660

tggaaataca tctttcaact tcgaaaatag tatttcagta aatacgcaaa ctagtccttg      720

tactacaaaa tttaaatatg atatgtattc attaaaagag ttggaaaaag aagcaataac      780

aaattgtatg agtaattgca atggtaacat tgcaaaagct tctaaaattc tgggaataaa      840

tagaagtact ttgtatacaa aaataaaaaa atatcaaatt aatttttctt aaagtgtatg      900

taaacacaac tttgttgtaa aaagcaacat tattttctta aaaaatgttg ctttttacag      960

catttttcaa ttatatatat taaccttata aagtcctacc cccctaaatt caaccttttc     1020

atgataaaaa acatactggc acaacatttg cttatatatt ta                        1062


<210>  103
<211>  823
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  103
cgtattttta attgcgaact taagatttaa ttaatatcta ctatgagtaa gtcaacatat       60

atacctaaat tatgataaaa ttatatatta taatttcaaa ataaacataa ctataataat      120

acactaagat aaagctattt atctgatggc tacctactgt aacactccct cttctatcaa      180

agtgagagat aacagtagct acgcccctag ataattcatc taaacttagt gggagaaaca      240

aaactctaaa gagaaagcga ttcactttaa atcaaagatt tgagatatct gcttctccca      300

ctaagtaaga ttcattgata taaaaaggaa ggtaatctaa taatgtttaa accatttact      360

catagtgaaa tagtcagtag gtctcttaat agatgcatta aataccatat agaaaaaggt      420

ataccaaaac ctaaacgaac acttagtcgc aaagaattgg acaacttaat aaaagaaaac      480

aacgatatta taaaaatagc aaaaccattt atggaaatac tttatgattt tttaagtgga      540

tcaggtttct cattatatct cacagacaaa aatggaattg tattaactat cataggtgac      600

aaagatattg taatggagca ggcaaaggct ggaatagcag aaggtattga tctgagtgaa      660

caaagtgcag gtacaaatgc agcaggaact gctatttttg aaaatttgtc agttcaactt      720

tcaggcaaag aacattttat aaatactttt cagatttata cctgctctgc atctgtcata      780

cataacgaac aaggaaatat aatcggatgt ctaactttaa ctg                        823


<210>  104
<211>  39
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  104
attcatcctg caggacagtt aaaaagcata tctaacagt                              39


<210>  105
<211>  37
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  105
gactgcggcc gctaaatata taagcaaatg ttgtgcc                                37


<210>  106
<211>  34
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  106
atatgctagc gtatttttaa ttgcgaactt aaga                                   34


<210>  107
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  107
gactggcgcg ccagttaaag ttagacatcc gattat                                 36


<210>  108
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  108
ttggaatttt agctgtagat aacaa                                             25


<210>  109
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  109
taagtgattt tcaatggact ttact                                             25


<210>  110
<211>  344
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Intron targeting cassette

<400>  110
aagcttataa ttatccttag atatcaatct tgtgcgccca gatagggtgt taagtcaagt       60

agtttaaggt actactctgt aagataacac agaaaacagc caacctaacc gaaaagcgaa      120

agctgatacg ggaacagagc acggttggaa agcgatgagt tacctaaaga caatcgggta      180

cgactgagtc gcaatgttaa tcagatataa ggtataagtt gtgtttactg aacgcaagtt      240

tctaatttcg attatatctc gatagaggaa agtgtctgaa acctctagta caaagaaagg      300

taagttagca agattgactt atctgttatc accacatttg taca                       344


<210>  111
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  111
tgattttagg ccatgaagct gtagg                                             25


<210>  112
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  112
catgatttgt tcaactatat cacc                                              24


<210>  113
<211>  1005
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  113
catatgcact tttaggtaaa taagcatgct tccctgcttc cacagataaa tctggagata       60

atccaagcat aaccaagtct ttttttggac tttgtgaatt tttcaaaata ccgtaaaaaa      120

ctgaattaaa ttttgtatct acaccacaag agctatcaat tttacaatat actcctttta      180

cataaaaaat taacgttaga gtagtaataa caagaaatat attagtttta ttaaaaattt      240

gttttctatc aattttcact atccttagca taataagaac tacaagtggc aattcaacaa      300

aacattgggc tttagctcca agaaacaaaa tggacgagat aaatataaat aaaaattttt      360

tataagacct atcttctcta tgttttaaaa agtaaataat acttgaaata aacaaaagaa      420

aacctacaat catcattggt tctccataaa ggctgttaaa ccatacaata tagtttccat      480

ctactaatat tattatagat aatatactaa aaaaaactgc tgcagctata tttttaaaat      540

gaatacagct aaagcatata tataatcctg tcatatacaa aattaagtaa ataaaagcta      600

aaattctagt gtcaaaataa ttataaccaa ttaccttaca taataatttt ccaaatgtaa      660

taggataaat catgcttgta gttggaataa ttcctaaaag ccttgaaaaa ctggttggaa      720

gcattttata ttcagttaca acatacttaa accagtgagc tgaatctttc cctttggcat      780

ctgttaaacc agtagctttc attactcttt caaaatctcc ttgatctgct atacctggca      840

taggaggata aaaaagtata aataaagctg ctatgaaagc tcctaggata ctaataattg      900

gtatatatct acataaactt gaattttccc taagagacca cctattttct ttcatatttt      960

agtaataact ctcccctttc ctgggactta tccaaaaata taaca                     1005


<210>  114
<211>  959
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  114
attacaagtg agcatactta tgtttcatat ttttctaaat ataaccttga taccaatgta       60

atctttatta gaaaatacgg cactggagag ccaagtgata caatggtaga agcaatttgt      120

aaggatataa aagatattac ttataaaaga gtaattgcta ttggcggcgg aagtgtcctt      180

gacgtttcaa aattgtttgc attaaagaaa gtctcgccag tacttgattt atttgatcac      240

aaattagaat ttgtaaaaga taaggaattg atcctaattc caacaacttg cggaacaggc      300

agtgaagtaa ctaatatttc tattcttgaa ttgaagtcaa gacatacaaa attaggtctt      360

gctatagatg aactatatgc agattttgct gttatgattc cagaacttct agagaattta      420

ccctttaaat tttttgcaac tagttccatt gatgccttga ttcattccat tgaatccagt      480

gtttcaccaa aagctacaag ctatacagaa atgttttcct ataaagcaat ggaaatgatc      540

ttaaaaggat atcaggagat ttcaaaaaat ggcccagacg ccaggttttc cttgttagat      600

aaatttttac ttgcaagcaa ttatgctgga attgcatttg gcaatgcagg gtgtggtgca      660

gtacatgcta tgagttatcc tttaggtgct aattaccatg ttcctcatgg agaagcaaat      720

tatcaaatgt tcattggagt atttaaaacc tattatcgtt taaaaccaca aggtaaaatt      780

acaaaactaa ataaattctt agcatccatt ttaaactgca aagaaaatga agtttacatt      840

aaaatagaag agttattaaa tgtattgatt cctaaaaaac aattacgtga gtatggggta      900

aaagaaaaag aattaaagga atttacacaa agtgttatga ctaaacaagg tcgtttaat       959


<210>  115
<211>  39
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  115
attcatcctg caggcatatg cacttttagg taaataagc                              39


<210>  116
<211>  37
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  116
gactgcggcc gctgttatat ttttggataa gtcccag                                37


<210>  117
<211>  35
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  117
atatgctagc attacaagtg agcatactta tgttt                                  35


<210>  118
<211>  37
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  118
gactggcgcg ccattaaacg accttgttta gtcataa                                37


<210>  119
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  119
agaattttgc aagttttata ttgct                                             25


<210>  120
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  120
aagtcaagct ctaactttga aatat                                             25


<210>  121
<211>  1041
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  121
tacatacaca cactaaattt ttcgataaaa taaatttaaa aacaaataaa cttaaaatag       60

agtataaaaa aaacagacat acaccccaat ggggcgaagg tctgtcgctg taccacccaa      120

attattactc gtatatataa cggctgtgcc ggcataactt actttatata aagttcagtc      180

tgcaacttag gagtgatttt caacaactgt agcttatggg ttcccaccaa atccccattc      240

tctgaaagca tatttttgtt tactcttctc cgtcatcgtt tttttatttg cactaactat      300

actataaaaa aatatgtatg tcaacaattt ttttgaaata tataattaac tctataaaga      360

aatcctaaat aaaaaatcaa ggtacaattc aaatattttt acaatcttca gctcggttaa      420

atattttgat aagcctaagc aataaattct aaaaagctaa aagtttaaat tgagtatttg      480

cttttataaa attatgaaaa atatttttta cggtaatata atgtaagaaa aacataatgc      540

aataaaaaaa taaaaaaatt ttaaaaaaac tattgacatt attctcataa ggtattatta      600

tcgtcacata cactaaatat tgataaagta aatttcaaaa acaaataaat ttttcaaagt      660

gatttaaaac caattaggtt tattttagtt ttaataaaat aaatgatatt tattagtcat      720

atccatgggg gttatttcta attgatattt tgaattggta caattgtaga gtacaaagac      780

aatgataggg aagagtaaat aggaagtatt ttttagagag tgagattttg gtgaaaactc      840

ataaatatga ctattgaagg tagccttgga gtcgtgagct gaaactaagt aggctttacc      900

ggtaaaaccg ttattacttt gagtaaaaaa ttgggtggta ccgcgcgacc aaacttctcg      960

ccccaagcag agaatgttgg ttgttttttt atacaaaaaa ttagtggtaa ttgctcaaat     1020

gctggttctt aaaattgaaa t                                               1041


<210>  122
<211>  1016
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  122
agattcattg attaaattgg ggtaatattt taacagtata tcaataagaa aatacttatg       60

aataacgctt atgaataagg gggcttatca tttagcggat gacttaaatt tacaggagaa      120

gtaggggagg tacttttccc cactaattcc tgtaatgtaa atatctatct ttttaggggc      180

aaaataatta taggtagata ttacttgtaa ataaaaaagg gcttataaat ataaattttt      240

ttataaaatg tgcgaaaatt attacgaaat tatatatagg tattataaaa actatgatgg      300

agaagagtaa atagtggagt atttttagag aattgggata aggtgaaaac ccatgaatac      360

gaaacttttg aaaatcactc ctaagttaca agctgaaatt agtaagctgt gtcggtatta      420

ccgttattag aattagaaaa aagttgggtg gtaccgcaaa gcttcttgcc ctaggcaggc      480

ggttattttt ttacaaaaaa tttccagatt taaggaggat actaaaaatg aaaagtgatt      540

cagtaaaaaa ggggattaag gcagctccag caagagcact tatgtatgga atgggatata      600

caaaagagga aattgaaaga cctcttatag gaatagtaaa ttcacaaaac gaaatagttg      660

caggtcacat gcatttagat gaaatagcaa aagctgcaaa acttggagta gcaatgtctg      720

ggggtactcc tatagagttt cctgctattg cagtttgcga tggaattgca atgggtcatg      780

ttggaatgaa gtattctctt gcttcaagag aactaatagc agattcaatt gaagctatgg      840

caacagctca tggttttgac ggattggtac tcatacctaa ctgtgacaaa attgtacctg      900

gaatgcttat ggcagctgca agacttaata taccagctgt tgtagtaagt ggaggaccta      960

tgagggcagg taagctaaat aacaaagcac ttgattttag cacttgtatt gaaaag         1016


<210>  123
<211>  39
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  123
attcatcctg caggtacata cacacactaa atttttcga                              39


<210>  124
<211>  37
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  124
gactgcggcc gcatttcaat tttaagaacc agcattt                                37


<210>  125
<211>  35
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  125
atatgctagc agattcattg attaaattgg ggtaa                                  35


<210>  126
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  126
gactggcgcg ccttttcaat acaagtgcta aaatca                                 36


<210>  127
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  127
tgagagttag tatttactct caact                                             25


<210>  128
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  128
ttctttacac aatccattac ataca                                             25


<210>  129
<211>  978
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  129
taaggctata tttggcaatg aaataaataa aggcgatgta attgtaataa gatatgaagg       60

accaaaaggc ggacctggaa tgagagaaat gctttcacca acttctgcta tagcaggtat      120

gggtttagat aaagatgtag cacttttaac agatggaaga ttctcaggag ctacaagagg      180

ggcatctata ggccatgtgt caccagaagc tatggaaggt ggactaatag gacttgtaga      240

agaaggagat actatatttg tagatattac aaataaaaaa ttagagctaa aagtaagtga      300

ggaagaactt gaaaagagaa gaaagaacta tgtaaagcct gaacctaaga taaaaacagg      360

atatttatca agatatgcaa aattggttac ttctgcaaat acaggtgcag ttcttaaata      420

attggagttt cttaatgtac ttttaatttg taaatatact caactttcaa ctaaaaatat      480

ttcatatatg tgaaagttga gtaaatatat tatttaataa aaattcagaa taaactattg      540

acatttaagt tgttttatag taacatatac tcatatttaa ataataaaag ctttgacagg      600

gactattaaa tatgatgtat attttaaagc gagtgggatc tggtgtaaac ccataaatat      660

ttcatattga aactcaccct tgagctgtaa gctgaaatta tagtaagctg tgccggtgtg      720

aatcgttatt gaattaagta ataaaattgg gtggtaccgc gaacagactt ctcgcctcaa      780

gagaaaggct gtttttttgt gctaattttt aacctaaaat tacctatatt aattactttg      840

aattataaat atttagtgtg tatcaagttt aaatttgatt taagtaattt aatttttaga      900

aactatttat aaatgtaaat tagaaagtat aaaatacgta ttaatatatg aaagctttga      960

aagggataag tagatatt                                                    978


<210>  130
<211>  1002
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  130
aagttataga tgaaatgcca taagggtggt atgaaaatta aatttagagg agggacacag       60

acatggacaa tacaatatta ttgagtaaga tatcccaggg cttaatggaa tgctgtaaat      120

caaaaaattt ttcaagagta aataaattaa ctgtgaatgt aaatgaaaac agcaatatta      180

attcttgtaa tctttatgag tatcttaaaa attttaataa aggcatagta gatgaatcta      240

cagaaattaa aattgaaatt gaagatttgc cggatcaagt tgtaatcata agcagcatag      300

aaggtgatat atcacaagag tgcatataaa gtatgtataa ggtttccaca cgaaaaatca      360

aggaaatggg tatagatttg gtttttacac aaatctatac ccattcccac taagcaagat      420

taaatttctt tctaatcata ttaactaata cttttgctgt atcatcaact ccgccgaaca      480

ggctgctgtt aattaagatg tctttgtaaa gtacattatc actgtatgac tttgcataac      540

ttaggctgta tagctgacgc gcttcatcaa cttctttaat caacttatca gcttcatctg      600

gctttatatg aagtatattg atacaatttt cctttcgtat ctcataggga gcatagataa      660

atatgctgaa atgatttgaa tggtttctta aaatatagtc agaacacctt cctacaaata      720

tacaggatga tttatctgcc agatcacaga tgattttctt ttgggcttca aatatttctt      780

cttgtgtctt ttctgaacta gttcctagtg gaaatttcat ttttttgtat tcatttttat      840

ctatttcttc tccactgtca atagtagata caggcaaatt catctttttt gcagcttctt      900

caacgatatc cctgtcgtaa tattcaacgc ctaacaattc agccattttt ttagcaatgg      960

gacgtcccag actcccgaat tgacgggaaa tagttattac at                        1002


<210>  131
<211>  39
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  131
attcatcctg caggtaaggc tatatttggc aatgaaata                              39


<210>  132
<211>  37
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  132
gactgcggcc gcaatatcta cttatccctt tcaaagc                                37


<210>  133
<211>  35
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  133
atatgctagc aagttataga tgaaatgcca taagg                                  35


<210>  134
<211>  37
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  134
gactggcgcg ccatgtaata actatttccc gtcaatt                                37


<210>  135
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  135
ataaatgaag atgcacttac tgtta                                             25


<210>  136
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  136
aaaatttctc cattttacga tccta                                             25


<210>  137
<211>  1115
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  137
agtaaatatt acgcgtaaag tgttagagaa tgggaggttg tctctaaacg aaaagcttaa       60

ataattttac tttataattt aaagcttatg atgtgatatt gcttccgctg ttacattaaa      120

gagattttgt acttacctct tttctcttta atgtgactac attaaagaga aaagaggtgt      180

ttctatgtag attttttgct ctgtgtaaat taatttaata ttataacact gggggtaaaa      240

agtatgaaaa gtagaatgaa cacaaaattt cttgttacta ccgctgtttt tgttgccgtc      300

gcagttgttt tgagatcgtt ttctatagca atagctgcag gtggcatact cactatgaga      360

ataagttttg acgccatatg ttatataatg cctggtatat tatttggacc attatatggg      420

ggaatttcag gtggattaat cgatatactt ggctatataa taagacctat gggtggatac      480

atccctttgt ttactataac taatatagca gctggtattt tgcctgcact tatatggaga      540

tatattaaaa atgctaaaga atataaagta aggaattgtt atattgcttt ctttggattg      600

ctcttagtag taggtttttt taattttatc ataatgaaat ttgcatatca tactacttta      660

ggacaactgc tatcatcttt aggaaaaaaa tctcaatacc ttagtaccgg acttatgtta      720

ataggtgcta taggcgttat tatatttata ataaatgtat ttattaagaa aagcatggta      780

aaatcctatg attttgtaaa taacaattat tttaaattaa taattgcaat tggtatatct      840

ggaattttaa tatgcactat aaatacttat atattgctta tatttactcc tgctctcatt      900

gccaaaggct ttatgttctt atggatacct agaataattg aggctcttct tatgactata      960

gtaaattcct atataacctg tatgattatg tactgttata gcttatttca aggtagggta     1020

gtaaaaaaag cttaaaacgt aaaagaaatg gggtcaaaca aatgtcccca ttttttattt     1080

ctctgtttac tcttctacta aatctgatat agccg                                1115


<210>  138
<211>  956
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  138
atctaagtcc cccttttatt tatatattaa tcaattattt tatcaaataa tataataatt       60

tatacttagt tagactaaaa aatagattgc ttgttatttt tttcacatac ttatttattc      120

tttttttaca cttatttact tagctaagtt agaattacac cttttatatt atatttatat      180

aatataaaag accaaacttc tcccctctgg ggcgagaagt ttggtccgcg gtaccaccca      240

attttttact taattcaatt aacggctctg ccggtataac ttacttatat ttcagttaaa      300

caactccagg gtgattttca ctagctaata atttatgagc tttcacctaa cctcattctc      360

tttgaaatct ttcaccaatt acttttccct atcctcattt ttactatcta attttcaatt      420

taattacact ttcacattta gtatatattt taataatact atcttatttt ttaaatgtca      480

atagcttttt taaattttta gaaaataaat tcaaaaatat acatgaatac ctaggttgtt      540

aaaaaatcag accatataaa catggtctga taagcagaaa ttattttgct gcatcttgtg      600

tacttgcaaa atcttcatcg ttcataacac tatgactatt cccatataca aagtatatta      660

taagtccaac aacaagccat actgcaaatc tcaccaaagt taccttttgc agattatata      720

tcaagaaagc acaagctgcc atagcaaaaa caggtgtaac cggtgaaaat ggaactttaa      780

atgatctagg tctatccggt tctctttttc ttaaaactat aactgatgca gatactatta      840

taaatgctgc tagtgtacct atattagtta gttctgaaac aacacttatt ggtgtaaatc      900

cagctattat catagttata atgccaacaa gtaaagtact ttttacaggt gtatgg          956


<210>  139
<211>  39
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  139
attcatcctg caggagtaaa tattacgcgt aaagtgtta                              39


<210>  140
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  140
gactgcggcc gcggctatat cagatttagt agaaga                                 36


<210>  141
<211>  35
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  141
atatgctagc atctaagtcc cccttttatt tatat                                  35


<210>  142
<211>  35
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  142
gactggcgcg ccatacacct gtaaaaagta cttta                                  35


<210>  143
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  143
atgctggtat atcaaatgtt ttagt                                             25


<210>  144
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  144
attgcagtat cagctatatt aacag                                             25


<210>  145
<211>  353
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  145
aaaaaagctt ataattatcc ttagatggcg ctccggtgcg cccagatagg gtgttaagtc       60

aagtagttta aggtactact ctgtaagata acacagaaaa cagccaacct aaccgaaaag      120

cgaaagctga tacgggaaca gagcacggtt ggaaagcgat gagttaccta aagacaatcg      180

ggtacgactg agtcgcaatg ttaatcagat ataaggtata agttgtgttt actgaacgca      240

agtttctaat ttcgattcca tctcgataga ggaaagtgtc tgaaacctct agtacaaaga      300

aaggtaagtt aaccggagcg acttatctgt tatcaccaca tttgtacaat ctg             353


<210>  146
<211>  353
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  146
aaaaaagctt ataattatcc ttagttaacc aagcagtgcg cccagatagg gtgttaagtc       60

aagtagttta aggtactact ctgtaagata acacagaaaa cagccaacct aaccgaaaag      120

cgaaagctga tacgggaaca gagcacggtt ggaaagcgat gagttaccta aagacaatcg      180

ggtacgactg agtcgcaatg ttaatcagat ataaggtata agttgtgttt actgaacgca      240

agtttctaat ttcgatttta actcgataga ggaaagtgtc tgaaacctct agtacaaaga      300

aaggtaagtt atctgcttgg acttatctgt tatcaccaca tttgtacaat ctg             353


<210>  147
<211>  353
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  147
aaaaaagctt ataattatcc ttaagtgtct tatcggtgcg cccagatagg gtgttaagtc       60

aagtagttta aggtactact ctgtaagata acacagaaaa cagccaacct aaccgaaaag      120

cgaaagctga tacgggaaca gagcacggtt ggaaagcgat gagttaccta aagacaatcg      180

ggtacgactg agtcgcaatg ttaatcagat ataaggtata agttgtgttt actgaacgca      240

agtttctaat ttcgattaca cttcgataga ggaaagtgtc tgaaacctct agtacaaaga      300

aaggtaagtt atccgataag acttatctgt tatcaccaca tttgtacaat ctg             353


<210>  148
<211>  353
<212>  DNA
<213>  Clostridium autoethanogenum

<400>  148
aaaaaagctt ataattatcc ttacatgacc agcccgtgcg cccagatagg gtgttaagtc       60

aagtagttta aggtactact ctgtaagata acacagaaaa cagccaacct aaccgaaaag      120

cgaaagctga tacgggaaca gagcacggtt ggaaagcgat gagttaccta aagacaatcg      180

ggtacgactg agtcgcaatg ttaatcagat ataaggtata agttgtgttt actgaacgca      240

agtttctaat ttcggtttca tgtcgataga ggaaagtgtc tgaaacctct agtacaaaga      300

aaggtaagtt aaggggctgg acttatctgt tatcaccaca tttgtacaat ctg             353


<210>  149
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  149
cacaacccgt catgagcaag gtgc                                              24


<210>  150
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  150
tgtaattact aaatcagcc                                                    19


<210>  151
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  151
ggaagtcagg gacatgcaca tgct                                              24


<210>  152
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  152
cattttcagg agcatatcca gcttc                                             25


<210>  153
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  153
gtgcaggtgg cggagttata ctggc                                             25


<210>  154
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  154
ccatagttcc gaggcctcca g                                                 21


<210>  155
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  155
ggagctgaag tactattaaa atg                                               23


<210>  156
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic primer

<400>  156
ctacttcagc tgattgtacg tc                                                22


