                         SEQUENCE LISTING

<110>  Cargill, Incorporated
       Rush, Brian J
       Fosmer, Arlene  M
 
<120>  Methods for succinate production

<130>  33449.8073.WO00 (N00148WO01)

<150>  US 61/590,795
<151>  2012-01-25

<160>  169   

<170>  PatentIn version 3.5

<210>  1
<211>  1458
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(1458)

<400>  1
atg tta gct gct aga tca tta aag gca aga atg tca aca aga gct ttc         48
Met Leu Ala Ala Arg Ser Leu Lys Ala Arg Met Ser Thr Arg Ala Phe           
1               5                   10                  15                

tca act acc tca att gca aaa aga atc gaa aaa gat gca ttt ggt gac         96
Ser Thr Thr Ser Ile Ala Lys Arg Ile Glu Lys Asp Ala Phe Gly Asp           
            20                  25                  30                    

att gaa gtc cca aat gag aaa tat tgg ggt gct caa act caa aga tct        144
Ile Glu Val Pro Asn Glu Lys Tyr Trp Gly Ala Gln Thr Gln Arg Ser           
        35                  40                  45                        

tta caa aat ttc aaa att ggt ggt aag aga gaa gtt atg cca gaa cca        192
Leu Gln Asn Phe Lys Ile Gly Gly Lys Arg Glu Val Met Pro Glu Pro           
    50                  55                  60                            

atc atc aaa tct ttt ggt att tta aag aag gct act gct aag atc aat        240
Ile Ile Lys Ser Phe Gly Ile Leu Lys Lys Ala Thr Ala Lys Ile Asn           
65                  70                  75                  80            

gct gag tct ggt gct tta gac cca aag tta tct gaa gcc atc caa caa        288
Ala Glu Ser Gly Ala Leu Asp Pro Lys Leu Ser Glu Ala Ile Gln Gln           
                85                  90                  95                

gct gca acc gaa gtt tat gaa ggt aaa cta atg gac cat ttc cca tta        336
Ala Ala Thr Glu Val Tyr Glu Gly Lys Leu Met Asp His Phe Pro Leu           
            100                 105                 110                   

gtt gtc ttt caa acc ggt tct ggt act caa tct aac atg aat gcc aat        384
Val Val Phe Gln Thr Gly Ser Gly Thr Gln Ser Asn Met Asn Ala Asn           
        115                 120                 125                       

gaa gtc atc tct aat aga gca att gaa atc ttg ggt ggt gaa tta ggc        432
Glu Val Ile Ser Asn Arg Ala Ile Glu Ile Leu Gly Gly Glu Leu Gly           
    130                 135                 140                           

tct aaa act cca gtc cat cct aat gat cat gtt aat atg tcc caa tct        480
Ser Lys Thr Pro Val His Pro Asn Asp His Val Asn Met Ser Gln Ser           
145                 150                 155                 160           

tct aat gat act ttc cct act gtc atg cat att gca gca gtt aca gaa        528
Ser Asn Asp Thr Phe Pro Thr Val Met His Ile Ala Ala Val Thr Glu           
                165                 170                 175               

gtt tca tcc cat tta tta cca gaa tta act gca cta aga gat gca ttg        576
Val Ser Ser His Leu Leu Pro Glu Leu Thr Ala Leu Arg Asp Ala Leu           
            180                 185                 190                   

caa aag aaa tcc gat gaa ttt aag aat att atc aaa atc ggt aga acc        624
Gln Lys Lys Ser Asp Glu Phe Lys Asn Ile Ile Lys Ile Gly Arg Thr           
        195                 200                 205                       

cat tta caa gat gca act cct tta act tta ggt caa gaa ttt tct ggt        672
His Leu Gln Asp Ala Thr Pro Leu Thr Leu Gly Gln Glu Phe Ser Gly           
    210                 215                 220                           

tat gtt caa caa tgt act aat ggt atc aaa aga atc gaa att gct ctt        720
Tyr Val Gln Gln Cys Thr Asn Gly Ile Lys Arg Ile Glu Ile Ala Leu           
225                 230                 235                 240           

gaa cat ttg aga tac tta gct caa ggt ggt act gcc gtt ggt act ggt        768
Glu His Leu Arg Tyr Leu Ala Gln Gly Gly Thr Ala Val Gly Thr Gly           
                245                 250                 255               

ctt aac acc aag aaa ggt ttt gct gaa aag gtt gca aat gaa gtc act        816
Leu Asn Thr Lys Lys Gly Phe Ala Glu Lys Val Ala Asn Glu Val Thr           
            260                 265                 270                   

aaa ttg act ggt tta caa ttc tat acc gct cca aat aaa ttc gaa gcc        864
Lys Leu Thr Gly Leu Gln Phe Tyr Thr Ala Pro Asn Lys Phe Glu Ala           
        275                 280                 285                       

ctt gca gct cac gat gct gtt gtt gaa atg tct ggt gct ttg aat acc        912
Leu Ala Ala His Asp Ala Val Val Glu Met Ser Gly Ala Leu Asn Thr           
    290                 295                 300                           

gtt gca gtc tca tta ttc aaa atc gct caa gat atc aga tat ttg ggt        960
Val Ala Val Ser Leu Phe Lys Ile Ala Gln Asp Ile Arg Tyr Leu Gly           
305                 310                 315                 320           

tcc ggc cca aga tgt ggt tat ggt gaa ttg gct tta cca gaa aat gaa       1008
Ser Gly Pro Arg Cys Gly Tyr Gly Glu Leu Ala Leu Pro Glu Asn Glu           
                325                 330                 335               

cca ggt tct tcc atc atg ccg ggt aaa gtt aac cca act caa aac gaa       1056
Pro Gly Ser Ser Ile Met Pro Gly Lys Val Asn Pro Thr Gln Asn Glu           
            340                 345                 350                   

gct ttg act atg ctt tgt acc caa gtc ttt ggt aac cac tct tgt att       1104
Ala Leu Thr Met Leu Cys Thr Gln Val Phe Gly Asn His Ser Cys Ile           
        355                 360                 365                       

acc ttt gca ggt gct tca ggt caa ttc gaa ttg aat gtc ttt aag cca       1152
Thr Phe Ala Gly Ala Ser Gly Gln Phe Glu Leu Asn Val Phe Lys Pro           
    370                 375                 380                           

gtt atg atc tcc aac ttg tta tct tct att agg tta tta ggt gat ggt       1200
Val Met Ile Ser Asn Leu Leu Ser Ser Ile Arg Leu Leu Gly Asp Gly           
385                 390                 395                 400           

tgt aat tct ttt aga atc cac tgt gtt gaa ggt atc att gca aat acc       1248
Cys Asn Ser Phe Arg Ile His Cys Val Glu Gly Ile Ile Ala Asn Thr           
                405                 410                 415               

gac aag att gat aaa tta cta cat gaa tct ctc atg tta gtt act gct       1296
Asp Lys Ile Asp Lys Leu Leu His Glu Ser Leu Met Leu Val Thr Ala           
            420                 425                 430                   

ttg aac cca cac att ggt tac gat aag gct tcc aag att gca aag aat       1344
Leu Asn Pro His Ile Gly Tyr Asp Lys Ala Ser Lys Ile Ala Lys Asn           
        435                 440                 445                       

gca cac aag aag ggc ttg aca ttg aaa caa tct gca ttg gaa tta ggt       1392
Ala His Lys Lys Gly Leu Thr Leu Lys Gln Ser Ala Leu Glu Leu Gly           
    450                 455                 460                           

tac ttg acc gaa gaa caa ttc aat gaa tgg gtt aga cca gaa aac atg       1440
Tyr Leu Thr Glu Glu Gln Phe Asn Glu Trp Val Arg Pro Glu Asn Met           
465                 470                 475                 480           

att ggt cca aag gat taa                                               1458
Ile Gly Pro Lys Asp                                                       
                485                                                       


<210>  2
<211>  485
<212>  PRT
<213>  Issatchenkia orientalis

<400>  2

Met Leu Ala Ala Arg Ser Leu Lys Ala Arg Met Ser Thr Arg Ala Phe 
1               5                   10                  15      


Ser Thr Thr Ser Ile Ala Lys Arg Ile Glu Lys Asp Ala Phe Gly Asp 
            20                  25                  30          


Ile Glu Val Pro Asn Glu Lys Tyr Trp Gly Ala Gln Thr Gln Arg Ser 
        35                  40                  45              


Leu Gln Asn Phe Lys Ile Gly Gly Lys Arg Glu Val Met Pro Glu Pro 
    50                  55                  60                  


Ile Ile Lys Ser Phe Gly Ile Leu Lys Lys Ala Thr Ala Lys Ile Asn 
65                  70                  75                  80  


Ala Glu Ser Gly Ala Leu Asp Pro Lys Leu Ser Glu Ala Ile Gln Gln 
                85                  90                  95      


Ala Ala Thr Glu Val Tyr Glu Gly Lys Leu Met Asp His Phe Pro Leu 
            100                 105                 110         


Val Val Phe Gln Thr Gly Ser Gly Thr Gln Ser Asn Met Asn Ala Asn 
        115                 120                 125             


Glu Val Ile Ser Asn Arg Ala Ile Glu Ile Leu Gly Gly Glu Leu Gly 
    130                 135                 140                 


Ser Lys Thr Pro Val His Pro Asn Asp His Val Asn Met Ser Gln Ser 
145                 150                 155                 160 


Ser Asn Asp Thr Phe Pro Thr Val Met His Ile Ala Ala Val Thr Glu 
                165                 170                 175     


Val Ser Ser His Leu Leu Pro Glu Leu Thr Ala Leu Arg Asp Ala Leu 
            180                 185                 190         


Gln Lys Lys Ser Asp Glu Phe Lys Asn Ile Ile Lys Ile Gly Arg Thr 
        195                 200                 205             


His Leu Gln Asp Ala Thr Pro Leu Thr Leu Gly Gln Glu Phe Ser Gly 
    210                 215                 220                 


Tyr Val Gln Gln Cys Thr Asn Gly Ile Lys Arg Ile Glu Ile Ala Leu 
225                 230                 235                 240 


Glu His Leu Arg Tyr Leu Ala Gln Gly Gly Thr Ala Val Gly Thr Gly 
                245                 250                 255     


Leu Asn Thr Lys Lys Gly Phe Ala Glu Lys Val Ala Asn Glu Val Thr 
            260                 265                 270         


Lys Leu Thr Gly Leu Gln Phe Tyr Thr Ala Pro Asn Lys Phe Glu Ala 
        275                 280                 285             


Leu Ala Ala His Asp Ala Val Val Glu Met Ser Gly Ala Leu Asn Thr 
    290                 295                 300                 


Val Ala Val Ser Leu Phe Lys Ile Ala Gln Asp Ile Arg Tyr Leu Gly 
305                 310                 315                 320 


Ser Gly Pro Arg Cys Gly Tyr Gly Glu Leu Ala Leu Pro Glu Asn Glu 
                325                 330                 335     


Pro Gly Ser Ser Ile Met Pro Gly Lys Val Asn Pro Thr Gln Asn Glu 
            340                 345                 350         


Ala Leu Thr Met Leu Cys Thr Gln Val Phe Gly Asn His Ser Cys Ile 
        355                 360                 365             


Thr Phe Ala Gly Ala Ser Gly Gln Phe Glu Leu Asn Val Phe Lys Pro 
    370                 375                 380                 


Val Met Ile Ser Asn Leu Leu Ser Ser Ile Arg Leu Leu Gly Asp Gly 
385                 390                 395                 400 


Cys Asn Ser Phe Arg Ile His Cys Val Glu Gly Ile Ile Ala Asn Thr 
                405                 410                 415     


Asp Lys Ile Asp Lys Leu Leu His Glu Ser Leu Met Leu Val Thr Ala 
            420                 425                 430         


Leu Asn Pro His Ile Gly Tyr Asp Lys Ala Ser Lys Ile Ala Lys Asn 
        435                 440                 445             


Ala His Lys Lys Gly Leu Thr Leu Lys Gln Ser Ala Leu Glu Leu Gly 
    450                 455                 460                 


Tyr Leu Thr Glu Glu Gln Phe Asn Glu Trp Val Arg Pro Glu Asn Met 
465                 470                 475                 480 


Ile Gly Pro Lys Asp 
                485 


<210>  3
<211>  2652
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(2652)

<400>  3
atg aac gaa caa tat tcc gca ttg cgt agt aat gtc agt atg ctc ggc         48
Met Asn Glu Gln Tyr Ser Ala Leu Arg Ser Asn Val Ser Met Leu Gly           
1               5                   10                  15                

aaa gtg ctg gga gaa acc atc aag gat gcg ttg gga gaa cac att ctt         96
Lys Val Leu Gly Glu Thr Ile Lys Asp Ala Leu Gly Glu His Ile Leu           
            20                  25                  30                    

gaa cgc gta gaa act atc cgt aag ttg tcg aaa tct tca cgc gct ggc        144
Glu Arg Val Glu Thr Ile Arg Lys Leu Ser Lys Ser Ser Arg Ala Gly           
        35                  40                  45                        

aat gat gct aac cgc cag gag ttg ctc acc acc tta caa aat ttg tcg        192
Asn Asp Ala Asn Arg Gln Glu Leu Leu Thr Thr Leu Gln Asn Leu Ser           
    50                  55                  60                            

aac gac gag ctg ctg ccc gtt gcg cgt gcg ttt agt cag ttc ctg aac        240
Asn Asp Glu Leu Leu Pro Val Ala Arg Ala Phe Ser Gln Phe Leu Asn           
65                  70                  75                  80            

ctg gcc aac acc gcc gag caa tac cac agc att tcg ccg aaa ggc gaa        288
Leu Ala Asn Thr Ala Glu Gln Tyr His Ser Ile Ser Pro Lys Gly Glu           
                85                  90                  95                

gct gcc agc aac ccg gaa gtg atc gcc cgc acc ctg cgt aaa ctg aaa        336
Ala Ala Ser Asn Pro Glu Val Ile Ala Arg Thr Leu Arg Lys Leu Lys           
            100                 105                 110                   

aac cag ccg gaa ctg agc gaa gac acc atc aaa aaa gca gtg gaa tcg        384
Asn Gln Pro Glu Leu Ser Glu Asp Thr Ile Lys Lys Ala Val Glu Ser           
        115                 120                 125                       

ctg tcg ctg gaa ctg gtc ctc acg gct cac cca acc gaa att acc cgt        432
Leu Ser Leu Glu Leu Val Leu Thr Ala His Pro Thr Glu Ile Thr Arg           
    130                 135                 140                           

cgt aca ctg atc cac aaa atg gtg gaa gtg aac gcc tgt tta aaa cag        480
Arg Thr Leu Ile His Lys Met Val Glu Val Asn Ala Cys Leu Lys Gln           
145                 150                 155                 160           

ctc gat aac aaa gat atc gct gac tac gaa cac aac cag ctg atg cgt        528
Leu Asp Asn Lys Asp Ile Ala Asp Tyr Glu His Asn Gln Leu Met Arg           
                165                 170                 175               

cgc ctg cgc cag ttg atc gcc cag tca tgg cat acc gat gaa atc cgt        576
Arg Leu Arg Gln Leu Ile Ala Gln Ser Trp His Thr Asp Glu Ile Arg           
            180                 185                 190                   

aag ctg cgt cca agc ccg gta gat gaa gcc aaa tgg ggc ttt gcc gta        624
Lys Leu Arg Pro Ser Pro Val Asp Glu Ala Lys Trp Gly Phe Ala Val           
        195                 200                 205                       

gtg gaa aac agc ctg tgg caa ggc gta cca aat tac ctg cgc gaa ctg        672
Val Glu Asn Ser Leu Trp Gln Gly Val Pro Asn Tyr Leu Arg Glu Leu           
    210                 215                 220                           

aac gaa caa ctg gaa gag aac ctc ggc tac aaa ctg ccc gtc gaa ttt        720
Asn Glu Gln Leu Glu Glu Asn Leu Gly Tyr Lys Leu Pro Val Glu Phe           
225                 230                 235                 240           

gtt ccg gtc cgt ttt act tcg tgg atg ggc ggc gac cgc gac ggc aac        768
Val Pro Val Arg Phe Thr Ser Trp Met Gly Gly Asp Arg Asp Gly Asn           
                245                 250                 255               

ccg aac gtc act gcc gat atc acc cgc cac gtc ctg cta ctc agc cgc        816
Pro Asn Val Thr Ala Asp Ile Thr Arg His Val Leu Leu Leu Ser Arg           
            260                 265                 270                   

tgg aaa gcc acc gat ttg ttc ctg aaa gat att cag gtg ctg gtt tct        864
Trp Lys Ala Thr Asp Leu Phe Leu Lys Asp Ile Gln Val Leu Val Ser           
        275                 280                 285                       

gaa ctg tcg atg gtt gaa gcg acc cct gaa ctg ctg gcg ctg gtt ggc        912
Glu Leu Ser Met Val Glu Ala Thr Pro Glu Leu Leu Ala Leu Val Gly           
    290                 295                 300                           

gaa gaa ggt gcc gca gaa ccg tat cgc tat ctg atg aaa aac ctg cgt        960
Glu Glu Gly Ala Ala Glu Pro Tyr Arg Tyr Leu Met Lys Asn Leu Arg           
305                 310                 315                 320           

tct cgc ctg atg gcg aca cag gca tgg ctg gaa gcg cgc ctg aaa ggc       1008
Ser Arg Leu Met Ala Thr Gln Ala Trp Leu Glu Ala Arg Leu Lys Gly           
                325                 330                 335               

gaa gaa ctg cca aaa cca gaa ggc ctg ctg aca caa aac gaa gaa ctg       1056
Glu Glu Leu Pro Lys Pro Glu Gly Leu Leu Thr Gln Asn Glu Glu Leu           
            340                 345                 350                   

tgg gaa ccg ctc tac gct tgc tac cag tca ctt cag gcg tgt ggc atg       1104
Trp Glu Pro Leu Tyr Ala Cys Tyr Gln Ser Leu Gln Ala Cys Gly Met           
        355                 360                 365                       

ggt att atc gcc aac ggc gat ctg ctc gac acc ctg cgc cgc gtg aaa       1152
Gly Ile Ile Ala Asn Gly Asp Leu Leu Asp Thr Leu Arg Arg Val Lys           
    370                 375                 380                           

tgt ttc ggc gta ccg ctg gtc cgt att gat atc cgt cag gag agc acg       1200
Cys Phe Gly Val Pro Leu Val Arg Ile Asp Ile Arg Gln Glu Ser Thr           
385                 390                 395                 400           

cgt cat acc gaa gcg ctg ggc gag ctg acc cgc tac ctc ggt atc ggc       1248
Arg His Thr Glu Ala Leu Gly Glu Leu Thr Arg Tyr Leu Gly Ile Gly           
                405                 410                 415               

gac tac gaa agc tgg tca gag gcc gac aaa cag gcg ttc ctg atc cgc       1296
Asp Tyr Glu Ser Trp Ser Glu Ala Asp Lys Gln Ala Phe Leu Ile Arg           
            420                 425                 430                   

gaa ctg aac tcc aaa cgt ccg ctt ctg ccg cgc aac tgg caa cca agc       1344
Glu Leu Asn Ser Lys Arg Pro Leu Leu Pro Arg Asn Trp Gln Pro Ser           
        435                 440                 445                       

gcc gaa acg cgc gaa gtg ctc gat acc tgc cag gtg att gcc gaa gca       1392
Ala Glu Thr Arg Glu Val Leu Asp Thr Cys Gln Val Ile Ala Glu Ala           
    450                 455                 460                           

ccg caa ggc tcc att gcc gcc tac gtg atc tcg atg gcg aaa acg ccg       1440
Pro Gln Gly Ser Ile Ala Ala Tyr Val Ile Ser Met Ala Lys Thr Pro           
465                 470                 475                 480           

tcc gac gta ctg gct gtc cac ctg ctg ctg aaa gaa gcg ggt atc ggg       1488
Ser Asp Val Leu Ala Val His Leu Leu Leu Lys Glu Ala Gly Ile Gly           
                485                 490                 495               

ttt gcg atg ccg gtt gct ccg ctg ttt gaa acc ctc gat gat ctg aac       1536
Phe Ala Met Pro Val Ala Pro Leu Phe Glu Thr Leu Asp Asp Leu Asn           
            500                 505                 510                   

aac gcc aac gat gtc atg acc cag ctg ctc aat att gac tgg tat cgt       1584
Asn Ala Asn Asp Val Met Thr Gln Leu Leu Asn Ile Asp Trp Tyr Arg           
        515                 520                 525                       

ggc ctg att cag ggc aaa cag atg gtg atg att ggc tat tcc gac tca       1632
Gly Leu Ile Gln Gly Lys Gln Met Val Met Ile Gly Tyr Ser Asp Ser           
    530                 535                 540                           

gca aaa gat gcg gga gtg atg gca gct tcc tgg gcg caa tat cag gca       1680
Ala Lys Asp Ala Gly Val Met Ala Ala Ser Trp Ala Gln Tyr Gln Ala           
545                 550                 555                 560           

cag gat gca tta atc aaa acc tgc gaa aaa gcg ggt att gag ctg acg       1728
Gln Asp Ala Leu Ile Lys Thr Cys Glu Lys Ala Gly Ile Glu Leu Thr           
                565                 570                 575               

ttg ttc cac ggt cgc ggc ggt tcc att ggt cgc ggc ggc gca cct gct       1776
Leu Phe His Gly Arg Gly Gly Ser Ile Gly Arg Gly Gly Ala Pro Ala           
            580                 585                 590                   

cat gcg gcg ctg ctg tca caa ccg cca gga agc ctg aaa ggc ggc ctg       1824
His Ala Ala Leu Leu Ser Gln Pro Pro Gly Ser Leu Lys Gly Gly Leu           
        595                 600                 605                       

cgc gta acc gaa cag ggc gag atg atc cgc ttt aaa tat ggt ctg cca       1872
Arg Val Thr Glu Gln Gly Glu Met Ile Arg Phe Lys Tyr Gly Leu Pro           
    610                 615                 620                           

gaa atc acc gtc agc agc ctg tcg ctt tat acc ggg gcg att ctg gaa       1920
Glu Ile Thr Val Ser Ser Leu Ser Leu Tyr Thr Gly Ala Ile Leu Glu           
625                 630                 635                 640           

gcc aac ctg ctg cca ccg ccg gag ccg aaa gag agc tgg cgt cgc att       1968
Ala Asn Leu Leu Pro Pro Pro Glu Pro Lys Glu Ser Trp Arg Arg Ile           
                645                 650                 655               

atg gat gaa ctg tca gtc atc tcc tgc gat gtc tac cgc ggc tac gta       2016
Met Asp Glu Leu Ser Val Ile Ser Cys Asp Val Tyr Arg Gly Tyr Val           
            660                 665                 670                   

cgt gaa aac aaa gat ttt gtg cct tac ttc cgc tcc gct acg ccg gaa       2064
Arg Glu Asn Lys Asp Phe Val Pro Tyr Phe Arg Ser Ala Thr Pro Glu           
        675                 680                 685                       

caa gaa ctg ggc aaa ctg ccg ttg ggt tca cgt ccg gcg aaa cgt cgc       2112
Gln Glu Leu Gly Lys Leu Pro Leu Gly Ser Arg Pro Ala Lys Arg Arg           
    690                 695                 700                           

cca acc ggc ggc gtc gag tca cta cgc gcc att ccg tgg atc ttc gcc       2160
Pro Thr Gly Gly Val Glu Ser Leu Arg Ala Ile Pro Trp Ile Phe Ala           
705                 710                 715                 720           

tgg acg caa aac cgt ctg atg ctc ccc gcc tgg ctg ggt gca ggt acg       2208
Trp Thr Gln Asn Arg Leu Met Leu Pro Ala Trp Leu Gly Ala Gly Thr           
                725                 730                 735               

gcg ctg caa aaa gtg gtc gaa gac ggc aaa cag agc gag ctg gag gct       2256
Ala Leu Gln Lys Val Val Glu Asp Gly Lys Gln Ser Glu Leu Glu Ala           
            740                 745                 750                   

atg tgc cgc gat tgg cca ttc ttc tcg acg cgt ctc ggc atg ctg gag       2304
Met Cys Arg Asp Trp Pro Phe Phe Ser Thr Arg Leu Gly Met Leu Glu           
        755                 760                 765                       

atg gtc ttc gcc aaa gca gac ctg tgg ctg gcg gaa tac tat gac caa       2352
Met Val Phe Ala Lys Ala Asp Leu Trp Leu Ala Glu Tyr Tyr Asp Gln           
    770                 775                 780                           

cgc ctg gta gac aaa gca ctg tgg ccg tta ggt aaa gag tta cgc aac       2400
Arg Leu Val Asp Lys Ala Leu Trp Pro Leu Gly Lys Glu Leu Arg Asn           
785                 790                 795                 800           

ctg caa gaa gaa gac atc aaa gtg gtg ctg gcg att gcc aac gat tcc       2448
Leu Gln Glu Glu Asp Ile Lys Val Val Leu Ala Ile Ala Asn Asp Ser           
                805                 810                 815               

cat ctg atg gcc gat ctg ccg tgg att gca gag tct att cag cta cgg       2496
His Leu Met Ala Asp Leu Pro Trp Ile Ala Glu Ser Ile Gln Leu Arg           
            820                 825                 830                   

aat att tac acc gac ccg ctg aac gta ttg cag gcc gag ttg ctg cac       2544
Asn Ile Tyr Thr Asp Pro Leu Asn Val Leu Gln Ala Glu Leu Leu His           
        835                 840                 845                       

cgc tcc cgc cag gca gaa aaa gaa ggc cag gaa ccg gat cct cgc gtc       2592
Arg Ser Arg Gln Ala Glu Lys Glu Gly Gln Glu Pro Asp Pro Arg Val           
    850                 855                 860                           

gaa caa gcg tta atg gtc act att gcc ggg att gcg gca ggt atg cgt       2640
Glu Gln Ala Leu Met Val Thr Ile Ala Gly Ile Ala Ala Gly Met Arg           
865                 870                 875                 880           

aat acc ggc taa                                                       2652
Asn Thr Gly                                                               
                                                                          


<210>  4
<211>  883
<212>  PRT
<213>  Escherichia coli

<400>  4

Met Asn Glu Gln Tyr Ser Ala Leu Arg Ser Asn Val Ser Met Leu Gly 
1               5                   10                  15      


Lys Val Leu Gly Glu Thr Ile Lys Asp Ala Leu Gly Glu His Ile Leu 
            20                  25                  30          


Glu Arg Val Glu Thr Ile Arg Lys Leu Ser Lys Ser Ser Arg Ala Gly 
        35                  40                  45              


Asn Asp Ala Asn Arg Gln Glu Leu Leu Thr Thr Leu Gln Asn Leu Ser 
    50                  55                  60                  


Asn Asp Glu Leu Leu Pro Val Ala Arg Ala Phe Ser Gln Phe Leu Asn 
65                  70                  75                  80  


Leu Ala Asn Thr Ala Glu Gln Tyr His Ser Ile Ser Pro Lys Gly Glu 
                85                  90                  95      


Ala Ala Ser Asn Pro Glu Val Ile Ala Arg Thr Leu Arg Lys Leu Lys 
            100                 105                 110         


Asn Gln Pro Glu Leu Ser Glu Asp Thr Ile Lys Lys Ala Val Glu Ser 
        115                 120                 125             


Leu Ser Leu Glu Leu Val Leu Thr Ala His Pro Thr Glu Ile Thr Arg 
    130                 135                 140                 


Arg Thr Leu Ile His Lys Met Val Glu Val Asn Ala Cys Leu Lys Gln 
145                 150                 155                 160 


Leu Asp Asn Lys Asp Ile Ala Asp Tyr Glu His Asn Gln Leu Met Arg 
                165                 170                 175     


Arg Leu Arg Gln Leu Ile Ala Gln Ser Trp His Thr Asp Glu Ile Arg 
            180                 185                 190         


Lys Leu Arg Pro Ser Pro Val Asp Glu Ala Lys Trp Gly Phe Ala Val 
        195                 200                 205             


Val Glu Asn Ser Leu Trp Gln Gly Val Pro Asn Tyr Leu Arg Glu Leu 
    210                 215                 220                 


Asn Glu Gln Leu Glu Glu Asn Leu Gly Tyr Lys Leu Pro Val Glu Phe 
225                 230                 235                 240 


Val Pro Val Arg Phe Thr Ser Trp Met Gly Gly Asp Arg Asp Gly Asn 
                245                 250                 255     


Pro Asn Val Thr Ala Asp Ile Thr Arg His Val Leu Leu Leu Ser Arg 
            260                 265                 270         


Trp Lys Ala Thr Asp Leu Phe Leu Lys Asp Ile Gln Val Leu Val Ser 
        275                 280                 285             


Glu Leu Ser Met Val Glu Ala Thr Pro Glu Leu Leu Ala Leu Val Gly 
    290                 295                 300                 


Glu Glu Gly Ala Ala Glu Pro Tyr Arg Tyr Leu Met Lys Asn Leu Arg 
305                 310                 315                 320 


Ser Arg Leu Met Ala Thr Gln Ala Trp Leu Glu Ala Arg Leu Lys Gly 
                325                 330                 335     


Glu Glu Leu Pro Lys Pro Glu Gly Leu Leu Thr Gln Asn Glu Glu Leu 
            340                 345                 350         


Trp Glu Pro Leu Tyr Ala Cys Tyr Gln Ser Leu Gln Ala Cys Gly Met 
        355                 360                 365             


Gly Ile Ile Ala Asn Gly Asp Leu Leu Asp Thr Leu Arg Arg Val Lys 
    370                 375                 380                 


Cys Phe Gly Val Pro Leu Val Arg Ile Asp Ile Arg Gln Glu Ser Thr 
385                 390                 395                 400 


Arg His Thr Glu Ala Leu Gly Glu Leu Thr Arg Tyr Leu Gly Ile Gly 
                405                 410                 415     


Asp Tyr Glu Ser Trp Ser Glu Ala Asp Lys Gln Ala Phe Leu Ile Arg 
            420                 425                 430         


Glu Leu Asn Ser Lys Arg Pro Leu Leu Pro Arg Asn Trp Gln Pro Ser 
        435                 440                 445             


Ala Glu Thr Arg Glu Val Leu Asp Thr Cys Gln Val Ile Ala Glu Ala 
    450                 455                 460                 


Pro Gln Gly Ser Ile Ala Ala Tyr Val Ile Ser Met Ala Lys Thr Pro 
465                 470                 475                 480 


Ser Asp Val Leu Ala Val His Leu Leu Leu Lys Glu Ala Gly Ile Gly 
                485                 490                 495     


Phe Ala Met Pro Val Ala Pro Leu Phe Glu Thr Leu Asp Asp Leu Asn 
            500                 505                 510         


Asn Ala Asn Asp Val Met Thr Gln Leu Leu Asn Ile Asp Trp Tyr Arg 
        515                 520                 525             


Gly Leu Ile Gln Gly Lys Gln Met Val Met Ile Gly Tyr Ser Asp Ser 
    530                 535                 540                 


Ala Lys Asp Ala Gly Val Met Ala Ala Ser Trp Ala Gln Tyr Gln Ala 
545                 550                 555                 560 


Gln Asp Ala Leu Ile Lys Thr Cys Glu Lys Ala Gly Ile Glu Leu Thr 
                565                 570                 575     


Leu Phe His Gly Arg Gly Gly Ser Ile Gly Arg Gly Gly Ala Pro Ala 
            580                 585                 590         


His Ala Ala Leu Leu Ser Gln Pro Pro Gly Ser Leu Lys Gly Gly Leu 
        595                 600                 605             


Arg Val Thr Glu Gln Gly Glu Met Ile Arg Phe Lys Tyr Gly Leu Pro 
    610                 615                 620                 


Glu Ile Thr Val Ser Ser Leu Ser Leu Tyr Thr Gly Ala Ile Leu Glu 
625                 630                 635                 640 


Ala Asn Leu Leu Pro Pro Pro Glu Pro Lys Glu Ser Trp Arg Arg Ile 
                645                 650                 655     


Met Asp Glu Leu Ser Val Ile Ser Cys Asp Val Tyr Arg Gly Tyr Val 
            660                 665                 670         


Arg Glu Asn Lys Asp Phe Val Pro Tyr Phe Arg Ser Ala Thr Pro Glu 
        675                 680                 685             


Gln Glu Leu Gly Lys Leu Pro Leu Gly Ser Arg Pro Ala Lys Arg Arg 
    690                 695                 700                 


Pro Thr Gly Gly Val Glu Ser Leu Arg Ala Ile Pro Trp Ile Phe Ala 
705                 710                 715                 720 


Trp Thr Gln Asn Arg Leu Met Leu Pro Ala Trp Leu Gly Ala Gly Thr 
                725                 730                 735     


Ala Leu Gln Lys Val Val Glu Asp Gly Lys Gln Ser Glu Leu Glu Ala 
            740                 745                 750         


Met Cys Arg Asp Trp Pro Phe Phe Ser Thr Arg Leu Gly Met Leu Glu 
        755                 760                 765             


Met Val Phe Ala Lys Ala Asp Leu Trp Leu Ala Glu Tyr Tyr Asp Gln 
    770                 775                 780                 


Arg Leu Val Asp Lys Ala Leu Trp Pro Leu Gly Lys Glu Leu Arg Asn 
785                 790                 795                 800 


Leu Gln Glu Glu Asp Ile Lys Val Val Leu Ala Ile Ala Asn Asp Ser 
                805                 810                 815     


His Leu Met Ala Asp Leu Pro Trp Ile Ala Glu Ser Ile Gln Leu Arg 
            820                 825                 830         


Asn Ile Tyr Thr Asp Pro Leu Asn Val Leu Gln Ala Glu Leu Leu His 
        835                 840                 845             


Arg Ser Arg Gln Ala Glu Lys Glu Gly Gln Glu Pro Asp Pro Arg Val 
    850                 855                 860                 


Glu Gln Ala Leu Met Val Thr Ile Ala Gly Ile Ala Ala Gly Met Arg 
865                 870                 875                 880 


Asn Thr Gly 
            


<210>  5
<211>  2643
<212>  DNA
<213>  Mannheimia succiniciproducens


<220>
<221>  CDS
<222>  (1)..(2643)

<400>  5
atg aca gaa gaa tat tta atg atg cgt aat aac atc aat atg ctg ggg         48
Met Thr Glu Glu Tyr Leu Met Met Arg Asn Asn Ile Asn Met Leu Gly           
1               5                   10                  15                

cgc ttt ttg ggc gaa act att cag gag gcg caa ggt gac gat att ctc         96
Arg Phe Leu Gly Glu Thr Ile Gln Glu Ala Gln Gly Asp Asp Ile Leu           
            20                  25                  30                    

gaa ctg att gaa aat atc cgc gta ctg tcc cgc aat tcc cgt agc ggc        144
Glu Leu Ile Glu Asn Ile Arg Val Leu Ser Arg Asn Ser Arg Ser Gly           
        35                  40                  45                        

gat gac aaa gcc cgg gcg gca tta tta gac acc ctt tcc act att tcg        192
Asp Asp Lys Ala Arg Ala Ala Leu Leu Asp Thr Leu Ser Thr Ile Ser           
    50                  55                  60                            

gcg gat aat att att ccg gtt gcc cgc gct ttc agc cag ttt ctg aac        240
Ala Asp Asn Ile Ile Pro Val Ala Arg Ala Phe Ser Gln Phe Leu Asn           
65                  70                  75                  80            

ctg aca aat gtg gcg gaa caa tat caa acc atg tct cgc tcc cat gaa        288
Leu Thr Asn Val Ala Glu Gln Tyr Gln Thr Met Ser Arg Ser His Glu           
                85                  90                  95                

gat aag gtt tct gcg gaa cgt tcc act gct gcg ctg ttc gcc cgc ctg        336
Asp Lys Val Ser Ala Glu Arg Ser Thr Ala Ala Leu Phe Ala Arg Leu           
            100                 105                 110                   

aaa gaa caa cat gtt tct cag gaa gaa atc att aaa acc gta cag aaa        384
Lys Glu Gln His Val Ser Gln Glu Glu Ile Ile Lys Thr Val Gln Lys           
        115                 120                 125                       

ctg ttg att gaa atc gtc ctt acc gct cac ccg acg gaa gtt acc cgc        432
Leu Leu Ile Glu Ile Val Leu Thr Ala His Pro Thr Glu Val Thr Arg           
    130                 135                 140                           

cgt tca tta atg cac aaa cag gtt gaa atc aac aaa tgt ctg gct cag        480
Arg Ser Leu Met His Lys Gln Val Glu Ile Asn Lys Cys Leu Ala Gln           
145                 150                 155                 160           

ctg gat cat acg gat tta acc gcc gaa gaa caa aaa aat att gag tat        528
Leu Asp His Thr Asp Leu Thr Ala Glu Glu Gln Lys Asn Ile Glu Tyr           
                165                 170                 175               

aaa tta ctt cgt ctt atc gcc gaa gcc tgg cat acc aat gaa atc cgt        576
Lys Leu Leu Arg Leu Ile Ala Glu Ala Trp His Thr Asn Glu Ile Arg           
            180                 185                 190                   

acc aat cgg ccg aca cct ctg gaa gaa gcc aaa tgg ggt ttt gcc gtt        624
Thr Asn Arg Pro Thr Pro Leu Glu Glu Ala Lys Trp Gly Phe Ala Val           
        195                 200                 205                       

atc gaa aac agt tta tgg gaa ggt ttg ccc gcc ttt atc cgc aaa ctt        672
Ile Glu Asn Ser Leu Trp Glu Gly Leu Pro Ala Phe Ile Arg Lys Leu           
    210                 215                 220                           

aac gat gcc gcc gtc gaa cat tta aat tat gct ttg ccg gta gac ctc        720
Asn Asp Ala Ala Val Glu His Leu Asn Tyr Ala Leu Pro Val Asp Leu           
225                 230                 235                 240           

aca ccg gta cgc ttc tct tcc tgg atg ggc ggt gac cgt gac ggc aac        768
Thr Pro Val Arg Phe Ser Ser Trp Met Gly Gly Asp Arg Asp Gly Asn           
                245                 250                 255               

ccc ttc gtt acc gca aaa att acc cgg gaa gcg ctg caa ctt gcg cgc        816
Pro Phe Val Thr Ala Lys Ile Thr Arg Glu Ala Leu Gln Leu Ala Arg           
            260                 265                 270                   

tgg aaa gcg gcg gat tta ttt tta acc gat att cag gaa ctc tgc gac        864
Trp Lys Ala Ala Asp Leu Phe Leu Thr Asp Ile Gln Glu Leu Cys Asp           
        275                 280                 285                       

gag ttg tca atg aca caa tgc act gcg gaa ttc cga gaa aaa tac ggt        912
Glu Leu Ser Met Thr Gln Cys Thr Ala Glu Phe Arg Glu Lys Tyr Gly           
    290                 295                 300                           

gat cat tta gaa ccc tat cgt gta gtt gtg aag gat tta cgc agc aaa        960
Asp His Leu Glu Pro Tyr Arg Val Val Val Lys Asp Leu Arg Ser Lys           
305                 310                 315                 320           

tta aaa aat acg ctg gat tat tac aac gat ata ctt gcg ggt cgc att       1008
Leu Lys Asn Thr Leu Asp Tyr Tyr Asn Asp Ile Leu Ala Gly Arg Ile           
                325                 330                 335               

ccg ccg ttt aaa caa gat gaa atc atc agt gaa gac caa caa ctc tgg       1056
Pro Pro Phe Lys Gln Asp Glu Ile Ile Ser Glu Asp Gln Gln Leu Trp           
            340                 345                 350                   

caa ccg ctt tat gac tgt tat caa tcc cta acc gcc tgc ggt atg cgt       1104
Gln Pro Leu Tyr Asp Cys Tyr Gln Ser Leu Thr Ala Cys Gly Met Arg           
        355                 360                 365                       

att att gcc aat gga tta ttg ctg gat acc tta cgc cgc gtt cgt tgt       1152
Ile Ile Ala Asn Gly Leu Leu Leu Asp Thr Leu Arg Arg Val Arg Cys           
    370                 375                 380                           

ttc ggc gtc aca tta ctg cgt tta gat atc cgt cag gaa agc acc cgc       1200
Phe Gly Val Thr Leu Leu Arg Leu Asp Ile Arg Gln Glu Ser Thr Arg           
385                 390                 395                 400           

cat agc gac gcc atc ggc gaa att acc cgc tac atc ggt tta ggc gat       1248
His Ser Asp Ala Ile Gly Glu Ile Thr Arg Tyr Ile Gly Leu Gly Asp           
                405                 410                 415               

tac agc caa tgg aca gaa gat gac aaa caa gcc ttc ctg atc cgg gaa       1296
Tyr Ser Gln Trp Thr Glu Asp Asp Lys Gln Ala Phe Leu Ile Arg Glu           
            420                 425                 430                   

tta agt tcc cgt cgt ccg cta att ccc cat aac tgg acg cct tcg gaa       1344
Leu Ser Ser Arg Arg Pro Leu Ile Pro His Asn Trp Thr Pro Ser Glu           
        435                 440                 445                       

cac act cgg gaa att tta gac acc tgt aaa gtc att gca aaa cag ccg       1392
His Thr Arg Glu Ile Leu Asp Thr Cys Lys Val Ile Ala Lys Gln Pro           
    450                 455                 460                           

gaa ggc gtt att tcc tgc tat atc att tcc atg gcg cgc acc gct tcc       1440
Glu Gly Val Ile Ser Cys Tyr Ile Ile Ser Met Ala Arg Thr Ala Ser           
465                 470                 475                 480           

gat gtt ttg gcg gtg cat tta tta ttg aaa gaa gcg ggc att tca tac       1488
Asp Val Leu Ala Val His Leu Leu Leu Lys Glu Ala Gly Ile Ser Tyr           
                485                 490                 495               

cat ctg ccg gta gtt cct cta ttt gaa aca ttg gac gac ctg gac gct       1536
His Leu Pro Val Val Pro Leu Phe Glu Thr Leu Asp Asp Leu Asp Ala           
            500                 505                 510                   

tct aaa gaa gtg atg acg caa ctg ttt aac gta ggc tgg tat cgc ggc       1584
Ser Lys Glu Val Met Thr Gln Leu Phe Asn Val Gly Trp Tyr Arg Gly           
        515                 520                 525                       

gta atc aaa aac cgc caa atg atc atg atc ggc tat tcc gat agc gcc       1632
Val Ile Lys Asn Arg Gln Met Ile Met Ile Gly Tyr Ser Asp Ser Ala           
    530                 535                 540                           

aaa gat gcg ggc atg atg gcg gcc tca tgg gcg caa tac cgg gcg cag       1680
Lys Asp Ala Gly Met Met Ala Ala Ser Trp Ala Gln Tyr Arg Ala Gln           
545                 550                 555                 560           

gac gct tta gtc aaa ctt tgc gaa caa acc ggc atc gaa ctt acc ctc       1728
Asp Ala Leu Val Lys Leu Cys Glu Gln Thr Gly Ile Glu Leu Thr Leu           
                565                 570                 575               

ttc cac ggc cgc ggc ggc acc gta gga cgt ggc ggt gca ccg gct cac       1776
Phe His Gly Arg Gly Gly Thr Val Gly Arg Gly Gly Ala Pro Ala His           
            580                 585                 590                   

gcc gca tta tta tcc caa ccg cca cgt tct ctg aaa aac ggc tta cgg       1824
Ala Ala Leu Leu Ser Gln Pro Pro Arg Ser Leu Lys Asn Gly Leu Arg           
        595                 600                 605                       

gta acc gaa caa ggg gaa atg atc cgc ttc aaa ctg gga tta ccg gct       1872
Val Thr Glu Gln Gly Glu Met Ile Arg Phe Lys Leu Gly Leu Pro Ala           
    610                 615                 620                           

atc gcc gca gaa agt ctg gat ctc tac gcc agc gcc att ctt gag gcc       1920
Ile Ala Ala Glu Ser Leu Asp Leu Tyr Ala Ser Ala Ile Leu Glu Ala           
625                 630                 635                 640           

aac ctc ctg ccg ccg ccg gaa ccg aaa gcc agc tgg tgc cgg gta atg       1968
Asn Leu Leu Pro Pro Pro Glu Pro Lys Ala Ser Trp Cys Arg Val Met           
                645                 650                 655               

gac gaa ctt gcc gtc gct tct tgc gaa atc tat cgc aat gtg gtg cgc       2016
Asp Glu Leu Ala Val Ala Ser Cys Glu Ile Tyr Arg Asn Val Val Arg           
            660                 665                 670                   

ggc gat aaa gat ttt gtg cct tac ttc cgc agc gcc aca ccg gaa cag       2064
Gly Asp Lys Asp Phe Val Pro Tyr Phe Arg Ser Ala Thr Pro Glu Gln           
        675                 680                 685                       

gaa ctg gca aaa ctg cct tta ggt tcc cga ccg gca aaa cgc aat ccg       2112
Glu Leu Ala Lys Leu Pro Leu Gly Ser Arg Pro Ala Lys Arg Asn Pro           
    690                 695                 700                           

aac ggc ggc gtt gaa agc ctg cgt gcc att ccc tgg atc ttc gcc tgg       2160
Asn Gly Gly Val Glu Ser Leu Arg Ala Ile Pro Trp Ile Phe Ala Trp           
705                 710                 715                 720           

atg caa aac cgc ctg atg ctg ccc gcc tgg ctc ggt gcc ggc gcc tca       2208
Met Gln Asn Arg Leu Met Leu Pro Ala Trp Leu Gly Ala Gly Ala Ser           
                725                 730                 735               

atc cgt cag gcg atg gaa agc ggc aaa gcg gcg gtg att gaa gaa atg       2256
Ile Arg Gln Ala Met Glu Ser Gly Lys Ala Ala Val Ile Glu Glu Met           
            740                 745                 750                   

tgc aac cat tgg ccg ttt ttc aat acc cga atc ggc atg ctt gaa atg       2304
Cys Asn His Trp Pro Phe Phe Asn Thr Arg Ile Gly Met Leu Glu Met           
        755                 760                 765                       

gta ttc agt aaa acc gat agc tgg ctg tcc gaa tat tac gac cag cgt       2352
Val Phe Ser Lys Thr Asp Ser Trp Leu Ser Glu Tyr Tyr Asp Gln Arg           
    770                 775                 780                           

tta gtg aaa aaa gag ctt tgg tat tta ggc gaa tcg ctg cgc aaa cag       2400
Leu Val Lys Lys Glu Leu Trp Tyr Leu Gly Glu Ser Leu Arg Lys Gln           
785                 790                 795                 800           

tta agc gaa gat atc gct acc gtg tta cgg ctt tcc ggc aaa ggc gat       2448
Leu Ser Glu Asp Ile Ala Thr Val Leu Arg Leu Ser Gly Lys Gly Asp           
                805                 810                 815               

caa tta atg tcg gat ttg cct tgg gtg gcg gaa tct att gca ctg cgt       2496
Gln Leu Met Ser Asp Leu Pro Trp Val Ala Glu Ser Ile Ala Leu Arg           
            820                 825                 830                   

aac gtt tac acc gac ccg tta aac tta ttg caa gtg gaa tta ttg cgt       2544
Asn Val Tyr Thr Asp Pro Leu Asn Leu Leu Gln Val Glu Leu Leu Arg           
        835                 840                 845                       

cgt ttg cga gcg gat ccc gaa cat ccg aat ccg gat atc gag caa gcg       2592
Arg Leu Arg Ala Asp Pro Glu His Pro Asn Pro Asp Ile Glu Gln Ala           
    850                 855                 860                           

ctg atg atc acc att acc ggt atc gcc gcg ggt atg cgt aat acg ggt       2640
Leu Met Ile Thr Ile Thr Gly Ile Ala Ala Gly Met Arg Asn Thr Gly           
865                 870                 875                 880           

tag                                                                   2643


<210>  6
<211>  880
<212>  PRT
<213>  Mannheimia succiniciproducens

<400>  6

Met Thr Glu Glu Tyr Leu Met Met Arg Asn Asn Ile Asn Met Leu Gly 
1               5                   10                  15      


Arg Phe Leu Gly Glu Thr Ile Gln Glu Ala Gln Gly Asp Asp Ile Leu 
            20                  25                  30          


Glu Leu Ile Glu Asn Ile Arg Val Leu Ser Arg Asn Ser Arg Ser Gly 
        35                  40                  45              


Asp Asp Lys Ala Arg Ala Ala Leu Leu Asp Thr Leu Ser Thr Ile Ser 
    50                  55                  60                  


Ala Asp Asn Ile Ile Pro Val Ala Arg Ala Phe Ser Gln Phe Leu Asn 
65                  70                  75                  80  


Leu Thr Asn Val Ala Glu Gln Tyr Gln Thr Met Ser Arg Ser His Glu 
                85                  90                  95      


Asp Lys Val Ser Ala Glu Arg Ser Thr Ala Ala Leu Phe Ala Arg Leu 
            100                 105                 110         


Lys Glu Gln His Val Ser Gln Glu Glu Ile Ile Lys Thr Val Gln Lys 
        115                 120                 125             


Leu Leu Ile Glu Ile Val Leu Thr Ala His Pro Thr Glu Val Thr Arg 
    130                 135                 140                 


Arg Ser Leu Met His Lys Gln Val Glu Ile Asn Lys Cys Leu Ala Gln 
145                 150                 155                 160 


Leu Asp His Thr Asp Leu Thr Ala Glu Glu Gln Lys Asn Ile Glu Tyr 
                165                 170                 175     


Lys Leu Leu Arg Leu Ile Ala Glu Ala Trp His Thr Asn Glu Ile Arg 
            180                 185                 190         


Thr Asn Arg Pro Thr Pro Leu Glu Glu Ala Lys Trp Gly Phe Ala Val 
        195                 200                 205             


Ile Glu Asn Ser Leu Trp Glu Gly Leu Pro Ala Phe Ile Arg Lys Leu 
    210                 215                 220                 


Asn Asp Ala Ala Val Glu His Leu Asn Tyr Ala Leu Pro Val Asp Leu 
225                 230                 235                 240 


Thr Pro Val Arg Phe Ser Ser Trp Met Gly Gly Asp Arg Asp Gly Asn 
                245                 250                 255     


Pro Phe Val Thr Ala Lys Ile Thr Arg Glu Ala Leu Gln Leu Ala Arg 
            260                 265                 270         


Trp Lys Ala Ala Asp Leu Phe Leu Thr Asp Ile Gln Glu Leu Cys Asp 
        275                 280                 285             


Glu Leu Ser Met Thr Gln Cys Thr Ala Glu Phe Arg Glu Lys Tyr Gly 
    290                 295                 300                 


Asp His Leu Glu Pro Tyr Arg Val Val Val Lys Asp Leu Arg Ser Lys 
305                 310                 315                 320 


Leu Lys Asn Thr Leu Asp Tyr Tyr Asn Asp Ile Leu Ala Gly Arg Ile 
                325                 330                 335     


Pro Pro Phe Lys Gln Asp Glu Ile Ile Ser Glu Asp Gln Gln Leu Trp 
            340                 345                 350         


Gln Pro Leu Tyr Asp Cys Tyr Gln Ser Leu Thr Ala Cys Gly Met Arg 
        355                 360                 365             


Ile Ile Ala Asn Gly Leu Leu Leu Asp Thr Leu Arg Arg Val Arg Cys 
    370                 375                 380                 


Phe Gly Val Thr Leu Leu Arg Leu Asp Ile Arg Gln Glu Ser Thr Arg 
385                 390                 395                 400 


His Ser Asp Ala Ile Gly Glu Ile Thr Arg Tyr Ile Gly Leu Gly Asp 
                405                 410                 415     


Tyr Ser Gln Trp Thr Glu Asp Asp Lys Gln Ala Phe Leu Ile Arg Glu 
            420                 425                 430         


Leu Ser Ser Arg Arg Pro Leu Ile Pro His Asn Trp Thr Pro Ser Glu 
        435                 440                 445             


His Thr Arg Glu Ile Leu Asp Thr Cys Lys Val Ile Ala Lys Gln Pro 
    450                 455                 460                 


Glu Gly Val Ile Ser Cys Tyr Ile Ile Ser Met Ala Arg Thr Ala Ser 
465                 470                 475                 480 


Asp Val Leu Ala Val His Leu Leu Leu Lys Glu Ala Gly Ile Ser Tyr 
                485                 490                 495     


His Leu Pro Val Val Pro Leu Phe Glu Thr Leu Asp Asp Leu Asp Ala 
            500                 505                 510         


Ser Lys Glu Val Met Thr Gln Leu Phe Asn Val Gly Trp Tyr Arg Gly 
        515                 520                 525             


Val Ile Lys Asn Arg Gln Met Ile Met Ile Gly Tyr Ser Asp Ser Ala 
    530                 535                 540                 


Lys Asp Ala Gly Met Met Ala Ala Ser Trp Ala Gln Tyr Arg Ala Gln 
545                 550                 555                 560 


Asp Ala Leu Val Lys Leu Cys Glu Gln Thr Gly Ile Glu Leu Thr Leu 
                565                 570                 575     


Phe His Gly Arg Gly Gly Thr Val Gly Arg Gly Gly Ala Pro Ala His 
            580                 585                 590         


Ala Ala Leu Leu Ser Gln Pro Pro Arg Ser Leu Lys Asn Gly Leu Arg 
        595                 600                 605             


Val Thr Glu Gln Gly Glu Met Ile Arg Phe Lys Leu Gly Leu Pro Ala 
    610                 615                 620                 


Ile Ala Ala Glu Ser Leu Asp Leu Tyr Ala Ser Ala Ile Leu Glu Ala 
625                 630                 635                 640 


Asn Leu Leu Pro Pro Pro Glu Pro Lys Ala Ser Trp Cys Arg Val Met 
                645                 650                 655     


Asp Glu Leu Ala Val Ala Ser Cys Glu Ile Tyr Arg Asn Val Val Arg 
            660                 665                 670         


Gly Asp Lys Asp Phe Val Pro Tyr Phe Arg Ser Ala Thr Pro Glu Gln 
        675                 680                 685             


Glu Leu Ala Lys Leu Pro Leu Gly Ser Arg Pro Ala Lys Arg Asn Pro 
    690                 695                 700                 


Asn Gly Gly Val Glu Ser Leu Arg Ala Ile Pro Trp Ile Phe Ala Trp 
705                 710                 715                 720 


Met Gln Asn Arg Leu Met Leu Pro Ala Trp Leu Gly Ala Gly Ala Ser 
                725                 730                 735     


Ile Arg Gln Ala Met Glu Ser Gly Lys Ala Ala Val Ile Glu Glu Met 
            740                 745                 750         


Cys Asn His Trp Pro Phe Phe Asn Thr Arg Ile Gly Met Leu Glu Met 
        755                 760                 765             


Val Phe Ser Lys Thr Asp Ser Trp Leu Ser Glu Tyr Tyr Asp Gln Arg 
    770                 775                 780                 


Leu Val Lys Lys Glu Leu Trp Tyr Leu Gly Glu Ser Leu Arg Lys Gln 
785                 790                 795                 800 


Leu Ser Glu Asp Ile Ala Thr Val Leu Arg Leu Ser Gly Lys Gly Asp 
                805                 810                 815     


Gln Leu Met Ser Asp Leu Pro Trp Val Ala Glu Ser Ile Ala Leu Arg 
            820                 825                 830         


Asn Val Tyr Thr Asp Pro Leu Asn Leu Leu Gln Val Glu Leu Leu Arg 
        835                 840                 845             


Arg Leu Arg Ala Asp Pro Glu His Pro Asn Pro Asp Ile Glu Gln Ala 
    850                 855                 860                 


Leu Met Ile Thr Ile Thr Gly Ile Ala Ala Gly Met Arg Asn Thr Gly 
865                 870                 875                 880 


<210>  7
<211>  3543
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(3543)

<400>  7
atg tca act gtg gaa gat cac tcc tcc cta cat aaa ttg aga aag gaa         48
Met Ser Thr Val Glu Asp His Ser Ser Leu His Lys Leu Arg Lys Glu           
1               5                   10                  15                

tct gag att ctt tcc aat gca aac aaa atc tta gtg gct aat aga ggt         96
Ser Glu Ile Leu Ser Asn Ala Asn Lys Ile Leu Val Ala Asn Arg Gly           
            20                  25                  30                    

gaa att cca att aga att ttc agg tca gcc cat gaa ttg tca atg cat        144
Glu Ile Pro Ile Arg Ile Phe Arg Ser Ala His Glu Leu Ser Met His           
        35                  40                  45                        

act gtg gcg atc tat tcc cat gaa gat cgg ttg tcc atg cat agg ttg        192
Thr Val Ala Ile Tyr Ser His Glu Asp Arg Leu Ser Met His Arg Leu           
    50                  55                  60                            

aag gcc gac gag gct tat gca atc ggt aag act ggt caa tat tcg cca        240
Lys Ala Asp Glu Ala Tyr Ala Ile Gly Lys Thr Gly Gln Tyr Ser Pro           
65                  70                  75                  80            

gtt caa gct tat cta caa att gac gaa att atc aaa ata gca aag gaa        288
Val Gln Ala Tyr Leu Gln Ile Asp Glu Ile Ile Lys Ile Ala Lys Glu           
                85                  90                  95                

cat gat gtt tcc atg atc cat cca ggt tat ggt ttc tta tct gaa aac        336
His Asp Val Ser Met Ile His Pro Gly Tyr Gly Phe Leu Ser Glu Asn           
            100                 105                 110                   

tcc gaa ttc gca aag aag gtt gaa gaa tcc ggt atg att tgg gtt ggg        384
Ser Glu Phe Ala Lys Lys Val Glu Glu Ser Gly Met Ile Trp Val Gly           
        115                 120                 125                       

cct cct gct gaa gtt att gat tct gtt ggt gac aag gtt tct gca aga        432
Pro Pro Ala Glu Val Ile Asp Ser Val Gly Asp Lys Val Ser Ala Arg           
    130                 135                 140                           

aat ttg gca att aaa tgt gac gtt cct gtt gtt cct ggt acc gat ggt        480
Asn Leu Ala Ile Lys Cys Asp Val Pro Val Val Pro Gly Thr Asp Gly           
145                 150                 155                 160           

cca att gaa gac att gaa cag gct aaa cag ttt gtg gaa caa tat ggt        528
Pro Ile Glu Asp Ile Glu Gln Ala Lys Gln Phe Val Glu Gln Tyr Gly           
                165                 170                 175               

tat cct gtc att ata aag gct gca ttt ggt ggt ggt ggt aga ggt atg        576
Tyr Pro Val Ile Ile Lys Ala Ala Phe Gly Gly Gly Gly Arg Gly Met           
            180                 185                 190                   

aga gtt gtt aga gaa ggt gat gat ata gtt gat gct ttc caa aga gcg        624
Arg Val Val Arg Glu Gly Asp Asp Ile Val Asp Ala Phe Gln Arg Ala           
        195                 200                 205                       

tca tct gaa gca aag tct gcc ttt ggt aat ggt act tgt ttt att gaa        672
Ser Ser Glu Ala Lys Ser Ala Phe Gly Asn Gly Thr Cys Phe Ile Glu           
    210                 215                 220                           

aga ttt ttg gat aag cca aaa cat att gag gtt caa tta ttg gct gat        720
Arg Phe Leu Asp Lys Pro Lys His Ile Glu Val Gln Leu Leu Ala Asp           
225                 230                 235                 240           

aat tat ggt aac aca atc cat ctc ttt gaa aga gat tgt tct gtt caa        768
Asn Tyr Gly Asn Thr Ile His Leu Phe Glu Arg Asp Cys Ser Val Gln           
                245                 250                 255               

aga aga cat caa aag gtt gtt gaa att gca cct gcc aaa act tta cct        816
Arg Arg His Gln Lys Val Val Glu Ile Ala Pro Ala Lys Thr Leu Pro           
            260                 265                 270                   

gtt gaa gtt aga aat gct ata tta aag gat gct gta acg tta gct aaa        864
Val Glu Val Arg Asn Ala Ile Leu Lys Asp Ala Val Thr Leu Ala Lys           
        275                 280                 285                       

acc gct aac tat aga aat gct ggt act gca gaa ttt tta gtt gat tcc        912
Thr Ala Asn Tyr Arg Asn Ala Gly Thr Ala Glu Phe Leu Val Asp Ser           
    290                 295                 300                           

caa aac aga cat tat ttt att gaa att aat cca aga att caa gtt gaa        960
Gln Asn Arg His Tyr Phe Ile Glu Ile Asn Pro Arg Ile Gln Val Glu           
305                 310                 315                 320           

cat aca att act gaa gaa atc acg ggt gtt gat att gtt gcc gct caa       1008
His Thr Ile Thr Glu Glu Ile Thr Gly Val Asp Ile Val Ala Ala Gln           
                325                 330                 335               

att caa att gct gca ggt gca tca ttg gaa caa ttg ggt cta tta caa       1056
Ile Gln Ile Ala Ala Gly Ala Ser Leu Glu Gln Leu Gly Leu Leu Gln           
            340                 345                 350                   

aac aaa att aca act aga ggt ttt gca att caa tgt aga att aca acc       1104
Asn Lys Ile Thr Thr Arg Gly Phe Ala Ile Gln Cys Arg Ile Thr Thr           
        355                 360                 365                       

gag gat cct gct aag aat ttt gcc cca gat aca ggt aaa att gag gtt       1152
Glu Asp Pro Ala Lys Asn Phe Ala Pro Asp Thr Gly Lys Ile Glu Val           
    370                 375                 380                           

tat aga tct gca ggt ggt aac ggt gtc aga tta gat ggt ggt aat ggg       1200
Tyr Arg Ser Ala Gly Gly Asn Gly Val Arg Leu Asp Gly Gly Asn Gly           
385                 390                 395                 400           

ttt gcc ggt gct gtt ata tct cct cat tat gac tcg atg ttg gtt aaa       1248
Phe Ala Gly Ala Val Ile Ser Pro His Tyr Asp Ser Met Leu Val Lys           
                405                 410                 415               

tgt tca aca tct ggt tct aac tat gaa att gcc aga aga aag atg att       1296
Cys Ser Thr Ser Gly Ser Asn Tyr Glu Ile Ala Arg Arg Lys Met Ile           
            420                 425                 430                   

aga gct tta gtt gaa ttt aga atc aga ggt gtc aag acc aat att cct       1344
Arg Ala Leu Val Glu Phe Arg Ile Arg Gly Val Lys Thr Asn Ile Pro           
        435                 440                 445                       

ttc tta ttg gca ttg cta act cat cca gtt ttc att tcg ggt gat tgt       1392
Phe Leu Leu Ala Leu Leu Thr His Pro Val Phe Ile Ser Gly Asp Cys           
    450                 455                 460                           

tgg aca act ttt att gat gat acc cct tcg tta ttc gaa atg gtt tct       1440
Trp Thr Thr Phe Ile Asp Asp Thr Pro Ser Leu Phe Glu Met Val Ser           
465                 470                 475                 480           

tca aag aat aga gcc caa aaa tta ttg gca tat att ggt gac ttg tgt       1488
Ser Lys Asn Arg Ala Gln Lys Leu Leu Ala Tyr Ile Gly Asp Leu Cys           
                485                 490                 495               

gtc aat ggt tct tca att aaa ggt caa att ggt ttc cct aaa ttg aac       1536
Val Asn Gly Ser Ser Ile Lys Gly Gln Ile Gly Phe Pro Lys Leu Asn           
            500                 505                 510                   

aag gaa gca gaa atc cca gat ttg ttg gat cca aat gat gag gtt att       1584
Lys Glu Ala Glu Ile Pro Asp Leu Leu Asp Pro Asn Asp Glu Val Ile           
        515                 520                 525                       

gat gtt tct aaa cct tct acc aat ggt cta aga ccg tat cta tta aag       1632
Asp Val Ser Lys Pro Ser Thr Asn Gly Leu Arg Pro Tyr Leu Leu Lys           
    530                 535                 540                           

tat gga cca gat gcg ttt tcc aaa aaa gtt cgt gaa ttc gat ggt tgt       1680
Tyr Gly Pro Asp Ala Phe Ser Lys Lys Val Arg Glu Phe Asp Gly Cys           
545                 550                 555                 560           

atg att atg gat acc acc tgg aga gat gca cat caa tca tta ttg gct       1728
Met Ile Met Asp Thr Thr Trp Arg Asp Ala His Gln Ser Leu Leu Ala           
                565                 570                 575               

aca aga gtt aga act att gat tta ctg aga att gct cca acg act agt       1776
Thr Arg Val Arg Thr Ile Asp Leu Leu Arg Ile Ala Pro Thr Thr Ser           
            580                 585                 590                   

cat gcc tta caa aat gca ttt gca tta gaa tgt tgg ggt ggc gca aca       1824
His Ala Leu Gln Asn Ala Phe Ala Leu Glu Cys Trp Gly Gly Ala Thr           
        595                 600                 605                       

ttt gat gtt gcg atg agg ttc ctc tat gaa gat cct tgg gag aga tta       1872
Phe Asp Val Ala Met Arg Phe Leu Tyr Glu Asp Pro Trp Glu Arg Leu           
    610                 615                 620                           

aga caa ctt aga aag gca gtt cca aat att cct ttc caa atg tta ttg       1920
Arg Gln Leu Arg Lys Ala Val Pro Asn Ile Pro Phe Gln Met Leu Leu           
625                 630                 635                 640           

aga ggt gct aat ggt gtt gct tat tcg tca tta cct gat aat gca att       1968
Arg Gly Ala Asn Gly Val Ala Tyr Ser Ser Leu Pro Asp Asn Ala Ile           
                645                 650                 655               

gat cat ttt gtt aag caa gca aag gat aat ggt gtt gat att ttc aga       2016
Asp His Phe Val Lys Gln Ala Lys Asp Asn Gly Val Asp Ile Phe Arg           
            660                 665                 670                   

gtc ttt gat gct ttg aac gat ttg gaa caa ttg aag gtt ggt gtt gat       2064
Val Phe Asp Ala Leu Asn Asp Leu Glu Gln Leu Lys Val Gly Val Asp           
        675                 680                 685                       

gct gtc aag aaa gcc gga ggt gtt gtt gaa gct aca gtt tgt tac tca       2112
Ala Val Lys Lys Ala Gly Gly Val Val Glu Ala Thr Val Cys Tyr Ser           
    690                 695                 700                           

ggt gat atg tta att cca ggt aaa aag tat aac ttg gat tat tat tta       2160
Gly Asp Met Leu Ile Pro Gly Lys Lys Tyr Asn Leu Asp Tyr Tyr Leu           
705                 710                 715                 720           

gag act gtt gga aag att gtg gaa atg ggt acc cat att tta ggt att       2208
Glu Thr Val Gly Lys Ile Val Glu Met Gly Thr His Ile Leu Gly Ile           
                725                 730                 735               

aag gat atg gct ggc acg tta aag cca aag gct gct aag ttg ttg att       2256
Lys Asp Met Ala Gly Thr Leu Lys Pro Lys Ala Ala Lys Leu Leu Ile           
            740                 745                 750                   

ggc tcg atc aga tca aaa tac cct gac ttg gtt atc cat gtc cat acc       2304
Gly Ser Ile Arg Ser Lys Tyr Pro Asp Leu Val Ile His Val His Thr           
        755                 760                 765                       

cat gac tct gct ggt acc ggt att tca act tat gtt gca tgc gca ttg       2352
His Asp Ser Ala Gly Thr Gly Ile Ser Thr Tyr Val Ala Cys Ala Leu           
    770                 775                 780                           

gca ggt gcc gac att gtc gat tgt gca atc aat tcg atg tct ggt tta       2400
Ala Gly Ala Asp Ile Val Asp Cys Ala Ile Asn Ser Met Ser Gly Leu           
785                 790                 795                 800           

acc tct caa cct tca atg agt gct ttt att gct gct tta gat ggt gat       2448
Thr Ser Gln Pro Ser Met Ser Ala Phe Ile Ala Ala Leu Asp Gly Asp           
                805                 810                 815               

atc gaa act ggt gtt cca gaa cat ttt gca aga caa tta gat gca tac       2496
Ile Glu Thr Gly Val Pro Glu His Phe Ala Arg Gln Leu Asp Ala Tyr           
            820                 825                 830                   

tgg gca gaa atg aga ttg tta tac tca tgt ttc gaa gcc gac ttg aag       2544
Trp Ala Glu Met Arg Leu Leu Tyr Ser Cys Phe Glu Ala Asp Leu Lys           
        835                 840                 845                       

gga cca gac cca gaa gtt tat aaa cat gaa att cca ggt gga cag ttg       2592
Gly Pro Asp Pro Glu Val Tyr Lys His Glu Ile Pro Gly Gly Gln Leu           
    850                 855                 860                           

act aac cta atc ttc caa gcc caa caa gtt ggt ttg ggt gaa caa tgg       2640
Thr Asn Leu Ile Phe Gln Ala Gln Gln Val Gly Leu Gly Glu Gln Trp           
865                 870                 875                 880           

gaa gaa act aag aag aag tat gaa gat gct aac atg ttg ttg ggt gat       2688
Glu Glu Thr Lys Lys Lys Tyr Glu Asp Ala Asn Met Leu Leu Gly Asp           
                885                 890                 895               

att gtc aag gtt acc cca acc tcc aag gtt gtt ggt gat tta gcc caa       2736
Ile Val Lys Val Thr Pro Thr Ser Lys Val Val Gly Asp Leu Ala Gln           
            900                 905                 910                   

ttt atg gtt tct aat aaa tta gaa aaa gaa gat gtt gaa aaa ctt gct       2784
Phe Met Val Ser Asn Lys Leu Glu Lys Glu Asp Val Glu Lys Leu Ala           
        915                 920                 925                       

aat gaa tta gat ttc cca gat tca gtt ctt gat ttc ttt gaa gga tta       2832
Asn Glu Leu Asp Phe Pro Asp Ser Val Leu Asp Phe Phe Glu Gly Leu           
    930                 935                 940                           

atg ggt aca cca tat ggt gga ttc cca gag cct ttg aga aca aat gtc       2880
Met Gly Thr Pro Tyr Gly Gly Phe Pro Glu Pro Leu Arg Thr Asn Val           
945                 950                 955                 960           

att tcc ggc aag aga aga aaa tta aag ggt aga cca ggt tta gaa tta       2928
Ile Ser Gly Lys Arg Arg Lys Leu Lys Gly Arg Pro Gly Leu Glu Leu           
                965                 970                 975               

gaa cct ttc aac ctc gag gaa atc aga gaa aat ttg gtt tcc aga ttt       2976
Glu Pro Phe Asn Leu Glu Glu Ile Arg Glu Asn Leu Val Ser Arg Phe           
            980                 985                 990                   

ggt cca ggt att act gaa tgt gat  gtt gca tct tat aac  atg tat cca     3024
Gly Pro Gly Ile Thr Glu Cys Asp  Val Ala Ser Tyr Asn  Met Tyr Pro         
        995                 1000                 1005                     

aag gtt  tac gag caa tat cgt  aag gtg gtt gaa aaa  tat ggt gat        3069
Lys Val  Tyr Glu Gln Tyr Arg  Lys Val Val Glu Lys  Tyr Gly Asp            
    1010                 1015                 1020                        

tta tct  gtt tta cca aca aaa  gca ttt ttg gct cct  cca act att        3114
Leu Ser  Val Leu Pro Thr Lys  Ala Phe Leu Ala Pro  Pro Thr Ile            
    1025                 1030                 1035                        

ggt gaa  gaa gtt cat gtg gaa  att gag caa ggt aag  act ttg att        3159
Gly Glu  Glu Val His Val Glu  Ile Glu Gln Gly Lys  Thr Leu Ile            
    1040                 1045                 1050                        

att aag  tta tta gcc att tct  gac ttg tct aaa tct  cat ggt aca        3204
Ile Lys  Leu Leu Ala Ile Ser  Asp Leu Ser Lys Ser  His Gly Thr            
    1055                 1060                 1065                        

aga gaa  gta tac ttt gaa ttg  aat ggt gaa atg aga  aag gtt aca        3249
Arg Glu  Val Tyr Phe Glu Leu  Asn Gly Glu Met Arg  Lys Val Thr            
    1070                 1075                 1080                        

att gaa  gat aaa aca gct gca  att gag act gtt aca  aga gca aag        3294
Ile Glu  Asp Lys Thr Ala Ala  Ile Glu Thr Val Thr  Arg Ala Lys            
    1085                 1090                 1095                        

gct gac  gga cac aat cca aat  gaa gtt ggt gcg cca  atg gct ggt        3339
Ala Asp  Gly His Asn Pro Asn  Glu Val Gly Ala Pro  Met Ala Gly            
    1100                 1105                 1110                        

gtc gtt  gtt gaa gtt aga gtg  aag cat gga aca gaa  gtt aag aag        3384
Val Val  Val Glu Val Arg Val  Lys His Gly Thr Glu  Val Lys Lys            
    1115                 1120                 1125                        

ggt gat  cca tta gcc gtt ttg  agt gca atg aaa atg  gaa atg gtt        3429
Gly Asp  Pro Leu Ala Val Leu  Ser Ala Met Lys Met  Glu Met Val            
    1130                 1135                 1140                        

att tct  gct cct gtt agt ggt  agg gtc ggt gaa gtt  ttt gtc aac        3474
Ile Ser  Ala Pro Val Ser Gly  Arg Val Gly Glu Val  Phe Val Asn            
    1145                 1150                 1155                        

gaa ggc  gat tcc gtt gat atg  ggt gat ttg ctt gtg  aaa att gcc        3519
Glu Gly  Asp Ser Val Asp Met  Gly Asp Leu Leu Val  Lys Ile Ala            
    1160                 1165                 1170                        

aaa gat  gaa gcg cca gca gct  taa                                     3543
Lys Asp  Glu Ala Pro Ala Ala                                              
    1175                 1180                                             


<210>  8
<211>  1180
<212>  PRT
<213>  Issatchenkia orientalis

<400>  8

Met Ser Thr Val Glu Asp His Ser Ser Leu His Lys Leu Arg Lys Glu 
1               5                   10                  15      


Ser Glu Ile Leu Ser Asn Ala Asn Lys Ile Leu Val Ala Asn Arg Gly 
            20                  25                  30          


Glu Ile Pro Ile Arg Ile Phe Arg Ser Ala His Glu Leu Ser Met His 
        35                  40                  45              


Thr Val Ala Ile Tyr Ser His Glu Asp Arg Leu Ser Met His Arg Leu 
    50                  55                  60                  


Lys Ala Asp Glu Ala Tyr Ala Ile Gly Lys Thr Gly Gln Tyr Ser Pro 
65                  70                  75                  80  


Val Gln Ala Tyr Leu Gln Ile Asp Glu Ile Ile Lys Ile Ala Lys Glu 
                85                  90                  95      


His Asp Val Ser Met Ile His Pro Gly Tyr Gly Phe Leu Ser Glu Asn 
            100                 105                 110         


Ser Glu Phe Ala Lys Lys Val Glu Glu Ser Gly Met Ile Trp Val Gly 
        115                 120                 125             


Pro Pro Ala Glu Val Ile Asp Ser Val Gly Asp Lys Val Ser Ala Arg 
    130                 135                 140                 


Asn Leu Ala Ile Lys Cys Asp Val Pro Val Val Pro Gly Thr Asp Gly 
145                 150                 155                 160 


Pro Ile Glu Asp Ile Glu Gln Ala Lys Gln Phe Val Glu Gln Tyr Gly 
                165                 170                 175     


Tyr Pro Val Ile Ile Lys Ala Ala Phe Gly Gly Gly Gly Arg Gly Met 
            180                 185                 190         


Arg Val Val Arg Glu Gly Asp Asp Ile Val Asp Ala Phe Gln Arg Ala 
        195                 200                 205             


Ser Ser Glu Ala Lys Ser Ala Phe Gly Asn Gly Thr Cys Phe Ile Glu 
    210                 215                 220                 


Arg Phe Leu Asp Lys Pro Lys His Ile Glu Val Gln Leu Leu Ala Asp 
225                 230                 235                 240 


Asn Tyr Gly Asn Thr Ile His Leu Phe Glu Arg Asp Cys Ser Val Gln 
                245                 250                 255     


Arg Arg His Gln Lys Val Val Glu Ile Ala Pro Ala Lys Thr Leu Pro 
            260                 265                 270         


Val Glu Val Arg Asn Ala Ile Leu Lys Asp Ala Val Thr Leu Ala Lys 
        275                 280                 285             


Thr Ala Asn Tyr Arg Asn Ala Gly Thr Ala Glu Phe Leu Val Asp Ser 
    290                 295                 300                 


Gln Asn Arg His Tyr Phe Ile Glu Ile Asn Pro Arg Ile Gln Val Glu 
305                 310                 315                 320 


His Thr Ile Thr Glu Glu Ile Thr Gly Val Asp Ile Val Ala Ala Gln 
                325                 330                 335     


Ile Gln Ile Ala Ala Gly Ala Ser Leu Glu Gln Leu Gly Leu Leu Gln 
            340                 345                 350         


Asn Lys Ile Thr Thr Arg Gly Phe Ala Ile Gln Cys Arg Ile Thr Thr 
        355                 360                 365             


Glu Asp Pro Ala Lys Asn Phe Ala Pro Asp Thr Gly Lys Ile Glu Val 
    370                 375                 380                 


Tyr Arg Ser Ala Gly Gly Asn Gly Val Arg Leu Asp Gly Gly Asn Gly 
385                 390                 395                 400 


Phe Ala Gly Ala Val Ile Ser Pro His Tyr Asp Ser Met Leu Val Lys 
                405                 410                 415     


Cys Ser Thr Ser Gly Ser Asn Tyr Glu Ile Ala Arg Arg Lys Met Ile 
            420                 425                 430         


Arg Ala Leu Val Glu Phe Arg Ile Arg Gly Val Lys Thr Asn Ile Pro 
        435                 440                 445             


Phe Leu Leu Ala Leu Leu Thr His Pro Val Phe Ile Ser Gly Asp Cys 
    450                 455                 460                 


Trp Thr Thr Phe Ile Asp Asp Thr Pro Ser Leu Phe Glu Met Val Ser 
465                 470                 475                 480 


Ser Lys Asn Arg Ala Gln Lys Leu Leu Ala Tyr Ile Gly Asp Leu Cys 
                485                 490                 495     


Val Asn Gly Ser Ser Ile Lys Gly Gln Ile Gly Phe Pro Lys Leu Asn 
            500                 505                 510         


Lys Glu Ala Glu Ile Pro Asp Leu Leu Asp Pro Asn Asp Glu Val Ile 
        515                 520                 525             


Asp Val Ser Lys Pro Ser Thr Asn Gly Leu Arg Pro Tyr Leu Leu Lys 
    530                 535                 540                 


Tyr Gly Pro Asp Ala Phe Ser Lys Lys Val Arg Glu Phe Asp Gly Cys 
545                 550                 555                 560 


Met Ile Met Asp Thr Thr Trp Arg Asp Ala His Gln Ser Leu Leu Ala 
                565                 570                 575     


Thr Arg Val Arg Thr Ile Asp Leu Leu Arg Ile Ala Pro Thr Thr Ser 
            580                 585                 590         


His Ala Leu Gln Asn Ala Phe Ala Leu Glu Cys Trp Gly Gly Ala Thr 
        595                 600                 605             


Phe Asp Val Ala Met Arg Phe Leu Tyr Glu Asp Pro Trp Glu Arg Leu 
    610                 615                 620                 


Arg Gln Leu Arg Lys Ala Val Pro Asn Ile Pro Phe Gln Met Leu Leu 
625                 630                 635                 640 


Arg Gly Ala Asn Gly Val Ala Tyr Ser Ser Leu Pro Asp Asn Ala Ile 
                645                 650                 655     


Asp His Phe Val Lys Gln Ala Lys Asp Asn Gly Val Asp Ile Phe Arg 
            660                 665                 670         


Val Phe Asp Ala Leu Asn Asp Leu Glu Gln Leu Lys Val Gly Val Asp 
        675                 680                 685             


Ala Val Lys Lys Ala Gly Gly Val Val Glu Ala Thr Val Cys Tyr Ser 
    690                 695                 700                 


Gly Asp Met Leu Ile Pro Gly Lys Lys Tyr Asn Leu Asp Tyr Tyr Leu 
705                 710                 715                 720 


Glu Thr Val Gly Lys Ile Val Glu Met Gly Thr His Ile Leu Gly Ile 
                725                 730                 735     


Lys Asp Met Ala Gly Thr Leu Lys Pro Lys Ala Ala Lys Leu Leu Ile 
            740                 745                 750         


Gly Ser Ile Arg Ser Lys Tyr Pro Asp Leu Val Ile His Val His Thr 
        755                 760                 765             


His Asp Ser Ala Gly Thr Gly Ile Ser Thr Tyr Val Ala Cys Ala Leu 
    770                 775                 780                 


Ala Gly Ala Asp Ile Val Asp Cys Ala Ile Asn Ser Met Ser Gly Leu 
785                 790                 795                 800 


Thr Ser Gln Pro Ser Met Ser Ala Phe Ile Ala Ala Leu Asp Gly Asp 
                805                 810                 815     


Ile Glu Thr Gly Val Pro Glu His Phe Ala Arg Gln Leu Asp Ala Tyr 
            820                 825                 830         


Trp Ala Glu Met Arg Leu Leu Tyr Ser Cys Phe Glu Ala Asp Leu Lys 
        835                 840                 845             


Gly Pro Asp Pro Glu Val Tyr Lys His Glu Ile Pro Gly Gly Gln Leu 
    850                 855                 860                 


Thr Asn Leu Ile Phe Gln Ala Gln Gln Val Gly Leu Gly Glu Gln Trp 
865                 870                 875                 880 


Glu Glu Thr Lys Lys Lys Tyr Glu Asp Ala Asn Met Leu Leu Gly Asp 
                885                 890                 895     


Ile Val Lys Val Thr Pro Thr Ser Lys Val Val Gly Asp Leu Ala Gln 
            900                 905                 910         


Phe Met Val Ser Asn Lys Leu Glu Lys Glu Asp Val Glu Lys Leu Ala 
        915                 920                 925             


Asn Glu Leu Asp Phe Pro Asp Ser Val Leu Asp Phe Phe Glu Gly Leu 
    930                 935                 940                 


Met Gly Thr Pro Tyr Gly Gly Phe Pro Glu Pro Leu Arg Thr Asn Val 
945                 950                 955                 960 


Ile Ser Gly Lys Arg Arg Lys Leu Lys Gly Arg Pro Gly Leu Glu Leu 
                965                 970                 975     


Glu Pro Phe Asn Leu Glu Glu Ile Arg Glu Asn Leu Val Ser Arg Phe 
            980                 985                 990         


Gly Pro Gly Ile Thr Glu Cys Asp  Val Ala Ser Tyr Asn  Met Tyr Pro 
        995                 1000                 1005             


Lys Val  Tyr Glu Gln Tyr Arg  Lys Val Val Glu Lys  Tyr Gly Asp 
    1010                 1015                 1020             


Leu Ser  Val Leu Pro Thr Lys  Ala Phe Leu Ala Pro  Pro Thr Ile 
    1025                 1030                 1035             


Gly Glu  Glu Val His Val Glu  Ile Glu Gln Gly Lys  Thr Leu Ile 
    1040                 1045                 1050             


Ile Lys  Leu Leu Ala Ile Ser  Asp Leu Ser Lys Ser  His Gly Thr 
    1055                 1060                 1065             


Arg Glu  Val Tyr Phe Glu Leu  Asn Gly Glu Met Arg  Lys Val Thr 
    1070                 1075                 1080             


Ile Glu  Asp Lys Thr Ala Ala  Ile Glu Thr Val Thr  Arg Ala Lys 
    1085                 1090                 1095             


Ala Asp  Gly His Asn Pro Asn  Glu Val Gly Ala Pro  Met Ala Gly 
    1100                 1105                 1110             


Val Val  Val Glu Val Arg Val  Lys His Gly Thr Glu  Val Lys Lys 
    1115                 1120                 1125             


Gly Asp  Pro Leu Ala Val Leu  Ser Ala Met Lys Met  Glu Met Val 
    1130                 1135                 1140             


Ile Ser  Ala Pro Val Ser Gly  Arg Val Gly Glu Val  Phe Val Asn 
    1145                 1150                 1155             


Glu Gly  Asp Ser Val Asp Met  Gly Asp Leu Leu Val  Lys Ile Ala 
    1160                 1165                 1170             


Lys Asp  Glu Ala Pro Ala Ala  
    1175                 1180 


<210>  9
<211>  3537
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(3537)

<400>  9
atg tcg caa aga aaa ttc gcc ggc ttg aga gat aac ttc aat ctc ttg         48
Met Ser Gln Arg Lys Phe Ala Gly Leu Arg Asp Asn Phe Asn Leu Leu           
1               5                   10                  15                

ggt gaa aag aac aaa ata ttg gtg gct aat aga gga gaa att cca atc         96
Gly Glu Lys Asn Lys Ile Leu Val Ala Asn Arg Gly Glu Ile Pro Ile           
            20                  25                  30                    

aga att ttt cgt acc gct cat gaa ctg tct atg cag acg gta gct ata        144
Arg Ile Phe Arg Thr Ala His Glu Leu Ser Met Gln Thr Val Ala Ile           
        35                  40                  45                        

tat tct cat gaa gat cgt ctt tca acg cac aaa caa aag gct gac gaa        192
Tyr Ser His Glu Asp Arg Leu Ser Thr His Lys Gln Lys Ala Asp Glu           
    50                  55                  60                            

gca tac gtc ata ggt gaa gta ggc caa tat acc ccc gtc ggc gct tat        240
Ala Tyr Val Ile Gly Glu Val Gly Gln Tyr Thr Pro Val Gly Ala Tyr           
65                  70                  75                  80            

ttg gcc att gac gaa atc att tcc att gcc caa aaa cac cag gta gat        288
Leu Ala Ile Asp Glu Ile Ile Ser Ile Ala Gln Lys His Gln Val Asp           
                85                  90                  95                

ttc atc cat cca ggt tat ggg ttc ttg tct gaa aat tcg gaa ttt gcc        336
Phe Ile His Pro Gly Tyr Gly Phe Leu Ser Glu Asn Ser Glu Phe Ala           
            100                 105                 110                   

gac aaa gta gtg aag gcc ggt atc act tgg att ggc cct cca gct gaa        384
Asp Lys Val Val Lys Ala Gly Ile Thr Trp Ile Gly Pro Pro Ala Glu           
        115                 120                 125                       

gtt att gac tcc gtg ggt gat aag gtc tca gct aga aac ctg gca gca        432
Val Ile Asp Ser Val Gly Asp Lys Val Ser Ala Arg Asn Leu Ala Ala           
    130                 135                 140                           

aaa gct aat gtg ccc acc gtt cct ggt aca cca ggt cct ata gaa act        480
Lys Ala Asn Val Pro Thr Val Pro Gly Thr Pro Gly Pro Ile Glu Thr           
145                 150                 155                 160           

gta gag gaa gca ctt gac ttc gtc aat gaa tac ggc tac ccg gtg atc        528
Val Glu Glu Ala Leu Asp Phe Val Asn Glu Tyr Gly Tyr Pro Val Ile           
                165                 170                 175               

att aag gcc gcc ttt ggt ggt ggt ggt aga ggt atg aga gtc gtt aga        576
Ile Lys Ala Ala Phe Gly Gly Gly Gly Arg Gly Met Arg Val Val Arg           
            180                 185                 190                   

gaa ggt gac gac gtg gca gat gcc ttt caa cgt gct acc tcc gaa gcc        624
Glu Gly Asp Asp Val Ala Asp Ala Phe Gln Arg Ala Thr Ser Glu Ala           
        195                 200                 205                       

cgt act gcc ttc ggt aat ggt acc tgc ttt gtg gaa aga ttc ttg gac        672
Arg Thr Ala Phe Gly Asn Gly Thr Cys Phe Val Glu Arg Phe Leu Asp           
    210                 215                 220                           

aag cca aag cat att gaa gtt caa ttg ttg gcc gat aac cac gga aac        720
Lys Pro Lys His Ile Glu Val Gln Leu Leu Ala Asp Asn His Gly Asn           
225                 230                 235                 240           

gtg gtt cat ctt ttc gaa aga gac tgt tcc gtg cag aga aga cac caa        768
Val Val His Leu Phe Glu Arg Asp Cys Ser Val Gln Arg Arg His Gln           
                245                 250                 255               

aag gtt gtc gaa gtg gcc cca gca aag act tta ccc cgt gaa gtc cgt        816
Lys Val Val Glu Val Ala Pro Ala Lys Thr Leu Pro Arg Glu Val Arg           
            260                 265                 270                   

gac gcc att ttg aca gat gca gtt aaa ttg gcc aaa gag tgt ggc tac        864
Asp Ala Ile Leu Thr Asp Ala Val Lys Leu Ala Lys Glu Cys Gly Tyr           
        275                 280                 285                       

aga aat gcg ggt act gct gaa ttc ttg gtt gat aac caa aat aga cac        912
Arg Asn Ala Gly Thr Ala Glu Phe Leu Val Asp Asn Gln Asn Arg His           
    290                 295                 300                           

tat ttc att gaa att aat cca aga atc caa gtg gaa cat acc atc aca        960
Tyr Phe Ile Glu Ile Asn Pro Arg Ile Gln Val Glu His Thr Ile Thr           
305                 310                 315                 320           

gaa gaa att acc ggt ata gat att gtg gcg gct cag atc caa att gcg       1008
Glu Glu Ile Thr Gly Ile Asp Ile Val Ala Ala Gln Ile Gln Ile Ala           
                325                 330                 335               

gca ggt gcc tct cta ccc cag ctg ggc cta ttc cag gac aaa att acg       1056
Ala Gly Ala Ser Leu Pro Gln Leu Gly Leu Phe Gln Asp Lys Ile Thr           
            340                 345                 350                   

act cgt ggc ttt gcc att cag tgc cgt att acc acg gaa gac cct gct       1104
Thr Arg Gly Phe Ala Ile Gln Cys Arg Ile Thr Thr Glu Asp Pro Ala           
        355                 360                 365                       

aag aac ttc caa cca gat acc ggt aga ata gaa gtg tac cgt tct gca       1152
Lys Asn Phe Gln Pro Asp Thr Gly Arg Ile Glu Val Tyr Arg Ser Ala           
    370                 375                 380                           

ggt ggt aat ggt gtt aga ctg gat ggt ggt aac gcc tat gca gga aca       1200
Gly Gly Asn Gly Val Arg Leu Asp Gly Gly Asn Ala Tyr Ala Gly Thr           
385                 390                 395                 400           

ata atc tca cct cat tac gac tca atg ctg gtc aaa tgc tca tgc tcc       1248
Ile Ile Ser Pro His Tyr Asp Ser Met Leu Val Lys Cys Ser Cys Ser           
                405                 410                 415               

ggt tcc acc tac gaa atc gtt cgt aga aaa atg att cgt gca tta atc       1296
Gly Ser Thr Tyr Glu Ile Val Arg Arg Lys Met Ile Arg Ala Leu Ile           
            420                 425                 430                   

gag ttc aga att aga ggt gtc aag acc aac att ccc ttc cta ttg act       1344
Glu Phe Arg Ile Arg Gly Val Lys Thr Asn Ile Pro Phe Leu Leu Thr           
        435                 440                 445                       

ctt ttg acc aat cca gta ttt att gag ggt aca tac tgg acg act ttt       1392
Leu Leu Thr Asn Pro Val Phe Ile Glu Gly Thr Tyr Trp Thr Thr Phe           
    450                 455                 460                           

att gac gac acc cca caa ctg ttc caa atg gtt tca tca caa aac aga       1440
Ile Asp Asp Thr Pro Gln Leu Phe Gln Met Val Ser Ser Gln Asn Arg           
465                 470                 475                 480           

gcc caa aaa ctt tta cat tac ctc gcc gac gtg gca gtc aat ggt tca       1488
Ala Gln Lys Leu Leu His Tyr Leu Ala Asp Val Ala Val Asn Gly Ser           
                485                 490                 495               

tct atc aag ggt caa att ggc ttg cca aaa tta aaa tca aat cca agt       1536
Ser Ile Lys Gly Gln Ile Gly Leu Pro Lys Leu Lys Ser Asn Pro Ser           
            500                 505                 510                   

gtc ccc cat ttg cac gat gct cag ggc aat gtc atc aac gtt aca aag       1584
Val Pro His Leu His Asp Ala Gln Gly Asn Val Ile Asn Val Thr Lys           
        515                 520                 525                       

tct gca cca cca tcc gga tgg agg caa gtg cta cta gaa aag ggg cca       1632
Ser Ala Pro Pro Ser Gly Trp Arg Gln Val Leu Leu Glu Lys Gly Pro           
    530                 535                 540                           

gct gaa ttt gcc aga caa gtt aga cag ttc aat ggt act tta ttg atg       1680
Ala Glu Phe Ala Arg Gln Val Arg Gln Phe Asn Gly Thr Leu Leu Met           
545                 550                 555                 560           

gac acc acc tgg aga gac gct cat caa tct cta ctt gca aca aga gtc       1728
Asp Thr Thr Trp Arg Asp Ala His Gln Ser Leu Leu Ala Thr Arg Val           
                565                 570                 575               

aga acc cac gat ttg gct aca atc gct cca aca acc gca cat gcc ctt       1776
Arg Thr His Asp Leu Ala Thr Ile Ala Pro Thr Thr Ala His Ala Leu           
            580                 585                 590                   

gca ggt cgt ttc gcc tta gaa tgt tgg ggt ggt gcc aca ttc gat gtt       1824
Ala Gly Arg Phe Ala Leu Glu Cys Trp Gly Gly Ala Thr Phe Asp Val           
        595                 600                 605                       

gca atg aga ttt ttg cat gag gat cca tgg gaa cgt ttg aga aaa tta       1872
Ala Met Arg Phe Leu His Glu Asp Pro Trp Glu Arg Leu Arg Lys Leu           
    610                 615                 620                           

aga tct ctg gtg cct aat att cca ttc caa atg tta ttg cgt ggt gcc       1920
Arg Ser Leu Val Pro Asn Ile Pro Phe Gln Met Leu Leu Arg Gly Ala           
625                 630                 635                 640           

aat ggt gtg gct tat tct tca ttg cct gac aat gct att gac cat ttc       1968
Asn Gly Val Ala Tyr Ser Ser Leu Pro Asp Asn Ala Ile Asp His Phe           
                645                 650                 655               

gtc aag caa gcc aag gat aat ggt gtt gat ata ttt aga gtc ttt gat       2016
Val Lys Gln Ala Lys Asp Asn Gly Val Asp Ile Phe Arg Val Phe Asp           
            660                 665                 670                   

gcc tta aat gac ttg gaa caa ttg aag gtc ggt gta gat gct gtg aag       2064
Ala Leu Asn Asp Leu Glu Gln Leu Lys Val Gly Val Asp Ala Val Lys           
        675                 680                 685                       

aag gca ggt ggt gtt gta gaa gcc act gtt tgt ttc tct ggg gat atg       2112
Lys Ala Gly Gly Val Val Glu Ala Thr Val Cys Phe Ser Gly Asp Met           
    690                 695                 700                           

ctt cag cca ggc aag aaa tac aat ttg gat tac tac ttg gaa att gct       2160
Leu Gln Pro Gly Lys Lys Tyr Asn Leu Asp Tyr Tyr Leu Glu Ile Ala           
705                 710                 715                 720           

gaa aaa att gtc caa atg ggc act cat atc ctg ggt atc aaa gat atg       2208
Glu Lys Ile Val Gln Met Gly Thr His Ile Leu Gly Ile Lys Asp Met           
                725                 730                 735               

gca ggt acc atg aag cca gca gct gcc aaa cta ctg att gga tct ttg       2256
Ala Gly Thr Met Lys Pro Ala Ala Ala Lys Leu Leu Ile Gly Ser Leu           
            740                 745                 750                   

agg gct aag tac cct gat ctc cca ata cat gtt cac act cac gat tct       2304
Arg Ala Lys Tyr Pro Asp Leu Pro Ile His Val His Thr His Asp Ser           
        755                 760                 765                       

gca ggt act gct gtt gca tca atg act gcg tgt gct ctg gcg ggc gcc       2352
Ala Gly Thr Ala Val Ala Ser Met Thr Ala Cys Ala Leu Ala Gly Ala           
    770                 775                 780                           

gat gtc gtt gat gtt gcc atc aac tca atg tct ggt tta act tca caa       2400
Asp Val Val Asp Val Ala Ile Asn Ser Met Ser Gly Leu Thr Ser Gln           
785                 790                 795                 800           

cca tca atc aat gct ctg ttg gct tca tta gaa ggt aat att gac act       2448
Pro Ser Ile Asn Ala Leu Leu Ala Ser Leu Glu Gly Asn Ile Asp Thr           
                805                 810                 815               

ggt att aac gtt gag cat gtc cgt gaa cta gat gca tat tgg gca gag       2496
Gly Ile Asn Val Glu His Val Arg Glu Leu Asp Ala Tyr Trp Ala Glu           
            820                 825                 830                   

atg aga ttg tta tac tct tgt ttc gag gct gac ttg aag ggc cca gat       2544
Met Arg Leu Leu Tyr Ser Cys Phe Glu Ala Asp Leu Lys Gly Pro Asp           
        835                 840                 845                       

cca gaa gtt tat caa cat gaa atc cca ggt ggt caa ttg aca aac ttg       2592
Pro Glu Val Tyr Gln His Glu Ile Pro Gly Gly Gln Leu Thr Asn Leu           
    850                 855                 860                           

ttg ttt caa gcc caa caa ttg ggt ctt gga gaa caa tgg gcc gaa aca       2640
Leu Phe Gln Ala Gln Gln Leu Gly Leu Gly Glu Gln Trp Ala Glu Thr           
865                 870                 875                 880           

aaa aga gct tac aga gaa gcc aat tat tta ttg ggt gat att gtc aaa       2688
Lys Arg Ala Tyr Arg Glu Ala Asn Tyr Leu Leu Gly Asp Ile Val Lys           
                885                 890                 895               

gtt acc cca act tcg aag gtc gtt ggt gat ctg gca caa ttt atg gtc       2736
Val Thr Pro Thr Ser Lys Val Val Gly Asp Leu Ala Gln Phe Met Val           
            900                 905                 910                   

tcc aat aaa tta act tcc gat gat gtg aga cgc ctg gct aat tct ttg       2784
Ser Asn Lys Leu Thr Ser Asp Asp Val Arg Arg Leu Ala Asn Ser Leu           
        915                 920                 925                       

gat ttc cct gac tct gtt atg gat ttc ttc gaa ggc tta atc ggc caa       2832
Asp Phe Pro Asp Ser Val Met Asp Phe Phe Glu Gly Leu Ile Gly Gln           
    930                 935                 940                           

cca tac ggt ggg ttc cca gaa cca ttt aga tca gac gtt tta agg aac       2880
Pro Tyr Gly Gly Phe Pro Glu Pro Phe Arg Ser Asp Val Leu Arg Asn           
945                 950                 955                 960           

aag aga aga aag ttg act tgt cgt cca ggc ctg gaa cta gag cca ttt       2928
Lys Arg Arg Lys Leu Thr Cys Arg Pro Gly Leu Glu Leu Glu Pro Phe           
                965                 970                 975               

gat ctc gaa aaa att aga gaa gac ttg cag aat aga ttt ggt gat gtt       2976
Asp Leu Glu Lys Ile Arg Glu Asp Leu Gln Asn Arg Phe Gly Asp Val           
            980                 985                 990                   

gat gag tgc gac gtt gct tct tat  aac atg tac cca aga  gtt tat gaa     3024
Asp Glu Cys Asp Val Ala Ser Tyr  Asn Met Tyr Pro Arg  Val Tyr Glu         
        995                 1000                 1005                     

gac ttc  caa aag atg aga gaa  acg tat ggt gat tta  tct gta ttg        3069
Asp Phe  Gln Lys Met Arg Glu  Thr Tyr Gly Asp Leu  Ser Val Leu            
    1010                 1015                 1020                        

cca aca  aga agc ttt ttg tct  cca cta gag act gac  gaa gaa att        3114
Pro Thr  Arg Ser Phe Leu Ser  Pro Leu Glu Thr Asp  Glu Glu Ile            
    1025                 1030                 1035                        

gaa gtt  gta atc gaa caa ggt  aaa acg cta att atc  aag cta cag        3159
Glu Val  Val Ile Glu Gln Gly  Lys Thr Leu Ile Ile  Lys Leu Gln            
    1040                 1045                 1050                        

gct gtg  ggt gat ttg aac aaa  aag acc ggt gaa aga  gaa gtt tac        3204
Ala Val  Gly Asp Leu Asn Lys  Lys Thr Gly Glu Arg  Glu Val Tyr            
    1055                 1060                 1065                        

ttt gat  ttg aat ggt gaa atg  aga aaa att cgt gtt  gct gac aga        3249
Phe Asp  Leu Asn Gly Glu Met  Arg Lys Ile Arg Val  Ala Asp Arg            
    1070                 1075                 1080                        

tca caa  aaa gtg gaa act gtt  act aaa tcc aaa gca  gac atg cat        3294
Ser Gln  Lys Val Glu Thr Val  Thr Lys Ser Lys Ala  Asp Met His            
    1085                 1090                 1095                        

gat cca  tta cac att ggt gca  cca atg gca ggt gtc  att gtt gaa        3339
Asp Pro  Leu His Ile Gly Ala  Pro Met Ala Gly Val  Ile Val Glu            
    1100                 1105                 1110                        

gtt aaa  gtt cat aaa gga tca  cta ata aag aag ggc  caa cct gta        3384
Val Lys  Val His Lys Gly Ser  Leu Ile Lys Lys Gly  Gln Pro Val            
    1115                 1120                 1125                        

gcc gta  tta agc gcc atg aaa  atg gaa atg att ata  tct tct cca        3429
Ala Val  Leu Ser Ala Met Lys  Met Glu Met Ile Ile  Ser Ser Pro            
    1130                 1135                 1140                        

tcc gat  gga caa gtt aaa gaa  gtg ttt gtc tct gat  ggt gaa aat        3474
Ser Asp  Gly Gln Val Lys Glu  Val Phe Val Ser Asp  Gly Glu Asn            
    1145                 1150                 1155                        

gtg gac  tct tct gat tta tta  gtt cta tta gaa gac  caa gtt cct        3519
Val Asp  Ser Ser Asp Leu Leu  Val Leu Leu Glu Asp  Gln Val Pro            
    1160                 1165                 1170                        

gtt gaa  act aag gca taa                                              3537
Val Glu  Thr Lys Ala                                                      
    1175                                                                  


<210>  10
<211>  1178
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  10

Met Ser Gln Arg Lys Phe Ala Gly Leu Arg Asp Asn Phe Asn Leu Leu 
1               5                   10                  15      


Gly Glu Lys Asn Lys Ile Leu Val Ala Asn Arg Gly Glu Ile Pro Ile 
            20                  25                  30          


Arg Ile Phe Arg Thr Ala His Glu Leu Ser Met Gln Thr Val Ala Ile 
        35                  40                  45              


Tyr Ser His Glu Asp Arg Leu Ser Thr His Lys Gln Lys Ala Asp Glu 
    50                  55                  60                  


Ala Tyr Val Ile Gly Glu Val Gly Gln Tyr Thr Pro Val Gly Ala Tyr 
65                  70                  75                  80  


Leu Ala Ile Asp Glu Ile Ile Ser Ile Ala Gln Lys His Gln Val Asp 
                85                  90                  95      


Phe Ile His Pro Gly Tyr Gly Phe Leu Ser Glu Asn Ser Glu Phe Ala 
            100                 105                 110         


Asp Lys Val Val Lys Ala Gly Ile Thr Trp Ile Gly Pro Pro Ala Glu 
        115                 120                 125             


Val Ile Asp Ser Val Gly Asp Lys Val Ser Ala Arg Asn Leu Ala Ala 
    130                 135                 140                 


Lys Ala Asn Val Pro Thr Val Pro Gly Thr Pro Gly Pro Ile Glu Thr 
145                 150                 155                 160 


Val Glu Glu Ala Leu Asp Phe Val Asn Glu Tyr Gly Tyr Pro Val Ile 
                165                 170                 175     


Ile Lys Ala Ala Phe Gly Gly Gly Gly Arg Gly Met Arg Val Val Arg 
            180                 185                 190         


Glu Gly Asp Asp Val Ala Asp Ala Phe Gln Arg Ala Thr Ser Glu Ala 
        195                 200                 205             


Arg Thr Ala Phe Gly Asn Gly Thr Cys Phe Val Glu Arg Phe Leu Asp 
    210                 215                 220                 


Lys Pro Lys His Ile Glu Val Gln Leu Leu Ala Asp Asn His Gly Asn 
225                 230                 235                 240 


Val Val His Leu Phe Glu Arg Asp Cys Ser Val Gln Arg Arg His Gln 
                245                 250                 255     


Lys Val Val Glu Val Ala Pro Ala Lys Thr Leu Pro Arg Glu Val Arg 
            260                 265                 270         


Asp Ala Ile Leu Thr Asp Ala Val Lys Leu Ala Lys Glu Cys Gly Tyr 
        275                 280                 285             


Arg Asn Ala Gly Thr Ala Glu Phe Leu Val Asp Asn Gln Asn Arg His 
    290                 295                 300                 


Tyr Phe Ile Glu Ile Asn Pro Arg Ile Gln Val Glu His Thr Ile Thr 
305                 310                 315                 320 


Glu Glu Ile Thr Gly Ile Asp Ile Val Ala Ala Gln Ile Gln Ile Ala 
                325                 330                 335     


Ala Gly Ala Ser Leu Pro Gln Leu Gly Leu Phe Gln Asp Lys Ile Thr 
            340                 345                 350         


Thr Arg Gly Phe Ala Ile Gln Cys Arg Ile Thr Thr Glu Asp Pro Ala 
        355                 360                 365             


Lys Asn Phe Gln Pro Asp Thr Gly Arg Ile Glu Val Tyr Arg Ser Ala 
    370                 375                 380                 


Gly Gly Asn Gly Val Arg Leu Asp Gly Gly Asn Ala Tyr Ala Gly Thr 
385                 390                 395                 400 


Ile Ile Ser Pro His Tyr Asp Ser Met Leu Val Lys Cys Ser Cys Ser 
                405                 410                 415     


Gly Ser Thr Tyr Glu Ile Val Arg Arg Lys Met Ile Arg Ala Leu Ile 
            420                 425                 430         


Glu Phe Arg Ile Arg Gly Val Lys Thr Asn Ile Pro Phe Leu Leu Thr 
        435                 440                 445             


Leu Leu Thr Asn Pro Val Phe Ile Glu Gly Thr Tyr Trp Thr Thr Phe 
    450                 455                 460                 


Ile Asp Asp Thr Pro Gln Leu Phe Gln Met Val Ser Ser Gln Asn Arg 
465                 470                 475                 480 


Ala Gln Lys Leu Leu His Tyr Leu Ala Asp Val Ala Val Asn Gly Ser 
                485                 490                 495     


Ser Ile Lys Gly Gln Ile Gly Leu Pro Lys Leu Lys Ser Asn Pro Ser 
            500                 505                 510         


Val Pro His Leu His Asp Ala Gln Gly Asn Val Ile Asn Val Thr Lys 
        515                 520                 525             


Ser Ala Pro Pro Ser Gly Trp Arg Gln Val Leu Leu Glu Lys Gly Pro 
    530                 535                 540                 


Ala Glu Phe Ala Arg Gln Val Arg Gln Phe Asn Gly Thr Leu Leu Met 
545                 550                 555                 560 


Asp Thr Thr Trp Arg Asp Ala His Gln Ser Leu Leu Ala Thr Arg Val 
                565                 570                 575     


Arg Thr His Asp Leu Ala Thr Ile Ala Pro Thr Thr Ala His Ala Leu 
            580                 585                 590         


Ala Gly Arg Phe Ala Leu Glu Cys Trp Gly Gly Ala Thr Phe Asp Val 
        595                 600                 605             


Ala Met Arg Phe Leu His Glu Asp Pro Trp Glu Arg Leu Arg Lys Leu 
    610                 615                 620                 


Arg Ser Leu Val Pro Asn Ile Pro Phe Gln Met Leu Leu Arg Gly Ala 
625                 630                 635                 640 


Asn Gly Val Ala Tyr Ser Ser Leu Pro Asp Asn Ala Ile Asp His Phe 
                645                 650                 655     


Val Lys Gln Ala Lys Asp Asn Gly Val Asp Ile Phe Arg Val Phe Asp 
            660                 665                 670         


Ala Leu Asn Asp Leu Glu Gln Leu Lys Val Gly Val Asp Ala Val Lys 
        675                 680                 685             


Lys Ala Gly Gly Val Val Glu Ala Thr Val Cys Phe Ser Gly Asp Met 
    690                 695                 700                 


Leu Gln Pro Gly Lys Lys Tyr Asn Leu Asp Tyr Tyr Leu Glu Ile Ala 
705                 710                 715                 720 


Glu Lys Ile Val Gln Met Gly Thr His Ile Leu Gly Ile Lys Asp Met 
                725                 730                 735     


Ala Gly Thr Met Lys Pro Ala Ala Ala Lys Leu Leu Ile Gly Ser Leu 
            740                 745                 750         


Arg Ala Lys Tyr Pro Asp Leu Pro Ile His Val His Thr His Asp Ser 
        755                 760                 765             


Ala Gly Thr Ala Val Ala Ser Met Thr Ala Cys Ala Leu Ala Gly Ala 
    770                 775                 780                 


Asp Val Val Asp Val Ala Ile Asn Ser Met Ser Gly Leu Thr Ser Gln 
785                 790                 795                 800 


Pro Ser Ile Asn Ala Leu Leu Ala Ser Leu Glu Gly Asn Ile Asp Thr 
                805                 810                 815     


Gly Ile Asn Val Glu His Val Arg Glu Leu Asp Ala Tyr Trp Ala Glu 
            820                 825                 830         


Met Arg Leu Leu Tyr Ser Cys Phe Glu Ala Asp Leu Lys Gly Pro Asp 
        835                 840                 845             


Pro Glu Val Tyr Gln His Glu Ile Pro Gly Gly Gln Leu Thr Asn Leu 
    850                 855                 860                 


Leu Phe Gln Ala Gln Gln Leu Gly Leu Gly Glu Gln Trp Ala Glu Thr 
865                 870                 875                 880 


Lys Arg Ala Tyr Arg Glu Ala Asn Tyr Leu Leu Gly Asp Ile Val Lys 
                885                 890                 895     


Val Thr Pro Thr Ser Lys Val Val Gly Asp Leu Ala Gln Phe Met Val 
            900                 905                 910         


Ser Asn Lys Leu Thr Ser Asp Asp Val Arg Arg Leu Ala Asn Ser Leu 
        915                 920                 925             


Asp Phe Pro Asp Ser Val Met Asp Phe Phe Glu Gly Leu Ile Gly Gln 
    930                 935                 940                 


Pro Tyr Gly Gly Phe Pro Glu Pro Phe Arg Ser Asp Val Leu Arg Asn 
945                 950                 955                 960 


Lys Arg Arg Lys Leu Thr Cys Arg Pro Gly Leu Glu Leu Glu Pro Phe 
                965                 970                 975     


Asp Leu Glu Lys Ile Arg Glu Asp Leu Gln Asn Arg Phe Gly Asp Val 
            980                 985                 990         


Asp Glu Cys Asp Val Ala Ser Tyr  Asn Met Tyr Pro Arg  Val Tyr Glu 
        995                 1000                 1005             


Asp Phe  Gln Lys Met Arg Glu  Thr Tyr Gly Asp Leu  Ser Val Leu 
    1010                 1015                 1020             


Pro Thr  Arg Ser Phe Leu Ser  Pro Leu Glu Thr Asp  Glu Glu Ile 
    1025                 1030                 1035             


Glu Val  Val Ile Glu Gln Gly  Lys Thr Leu Ile Ile  Lys Leu Gln 
    1040                 1045                 1050             


Ala Val  Gly Asp Leu Asn Lys  Lys Thr Gly Glu Arg  Glu Val Tyr 
    1055                 1060                 1065             


Phe Asp  Leu Asn Gly Glu Met  Arg Lys Ile Arg Val  Ala Asp Arg 
    1070                 1075                 1080             


Ser Gln  Lys Val Glu Thr Val  Thr Lys Ser Lys Ala  Asp Met His 
    1085                 1090                 1095             


Asp Pro  Leu His Ile Gly Ala  Pro Met Ala Gly Val  Ile Val Glu 
    1100                 1105                 1110             


Val Lys  Val His Lys Gly Ser  Leu Ile Lys Lys Gly  Gln Pro Val 
    1115                 1120                 1125             


Ala Val  Leu Ser Ala Met Lys  Met Glu Met Ile Ile  Ser Ser Pro 
    1130                 1135                 1140             


Ser Asp  Gly Gln Val Lys Glu  Val Phe Val Ser Asp  Gly Glu Asn 
    1145                 1150                 1155             


Val Asp  Ser Ser Asp Leu Leu  Val Leu Leu Glu Asp  Gln Val Pro 
    1160                 1165                 1170             


Val Glu  Thr Lys Ala 
    1175             


<210>  11
<211>  3528
<212>  DNA
<213>  Kluyveromyces marxianus


<220>
<221>  CDS
<222>  (1)..(3528)

<400>  11
atg tct acc caa aac gat ctg gcc ggg ttg cgt gat aac tcg aac cta         48
Met Ser Thr Gln Asn Asp Leu Ala Gly Leu Arg Asp Asn Ser Asn Leu           
1               5                   10                  15                

tta ggt gaa aag aac aag att ctt gtt gcc aac cgt ggt gaa att cca         96
Leu Gly Glu Lys Asn Lys Ile Leu Val Ala Asn Arg Gly Glu Ile Pro           
            20                  25                  30                    

att aga atc ttt aga acg gct cat gaa ctt tcg atg aag act gtt gcg        144
Ile Arg Ile Phe Arg Thr Ala His Glu Leu Ser Met Lys Thr Val Ala           
        35                  40                  45                        

atc tat tcg cac gag gat aga cta tct atg cac aga ttg aag gca gac        192
Ile Tyr Ser His Glu Asp Arg Leu Ser Met His Arg Leu Lys Ala Asp           
    50                  55                  60                            

gaa gct tac gtt att ggt gag cca gga aaa tac act cca gtt ggt gcg        240
Glu Ala Tyr Val Ile Gly Glu Pro Gly Lys Tyr Thr Pro Val Gly Ala           
65                  70                  75                  80            

tat ttg gcg atc gat gag att atc aag att gct caa ttg cac gga gtg        288
Tyr Leu Ala Ile Asp Glu Ile Ile Lys Ile Ala Gln Leu His Gly Val           
                85                  90                  95                

agc ttc atc cac cct ggt tat ggg ttc tta tcg gaa aac tct gag ttt        336
Ser Phe Ile His Pro Gly Tyr Gly Phe Leu Ser Glu Asn Ser Glu Phe           
            100                 105                 110                   

gcc aag aag gtg gcc gac tct ggt atc acg tgg gtt ggt cct cca gcc        384
Ala Lys Lys Val Ala Asp Ser Gly Ile Thr Trp Val Gly Pro Pro Ala           
        115                 120                 125                       

gat gtg atc gat gct gtt ggt gac aag gtt tct gcc aga aac ttg gcc        432
Asp Val Ile Asp Ala Val Gly Asp Lys Val Ser Ala Arg Asn Leu Ala           
    130                 135                 140                           

gag aga gcg gat gtt cca gtg gtt cca ggt acg cct ggt cca ata gag        480
Glu Arg Ala Asp Val Pro Val Val Pro Gly Thr Pro Gly Pro Ile Glu           
145                 150                 155                 160           

aca gtt gaa gaa gca gtt gaa ttt gtg gag aag tac gga tac cca gtc        528
Thr Val Glu Glu Ala Val Glu Phe Val Glu Lys Tyr Gly Tyr Pro Val           
                165                 170                 175               

atc atc aag gct gcc ttc ggt ggt ggt ggt cgt ggt atg aga gtt gtt        576
Ile Ile Lys Ala Ala Phe Gly Gly Gly Gly Arg Gly Met Arg Val Val           
            180                 185                 190                   

cgt gaa ggt gat gat atc gcc gat gct ttc caa aga gcc aag tcc gaa        624
Arg Glu Gly Asp Asp Ile Ala Asp Ala Phe Gln Arg Ala Lys Ser Glu           
        195                 200                 205                       

gct gtt act gct ttc ggt aac ggt act tgt ttc gtt gaa aga ttc ttg        672
Ala Val Thr Ala Phe Gly Asn Gly Thr Cys Phe Val Glu Arg Phe Leu           
    210                 215                 220                           

gac aag cca aag cac atc gaa gtt cag ttg ttg gct gat cac tac ggt        720
Asp Lys Pro Lys His Ile Glu Val Gln Leu Leu Ala Asp His Tyr Gly           
225                 230                 235                 240           

aat gtc atc cat cta ttc gaa aga gac tgt tct gtg caa aga aga cat        768
Asn Val Ile His Leu Phe Glu Arg Asp Cys Ser Val Gln Arg Arg His           
                245                 250                 255               

caa aag gtc gtt gaa gta gcg cca gcc aag act ttg cca gag agc gtg        816
Gln Lys Val Val Glu Val Ala Pro Ala Lys Thr Leu Pro Glu Ser Val           
            260                 265                 270                   

cgt aat gca atc ttg act gac gct gtc aag ttg gct aag gag gca gga        864
Arg Asn Ala Ile Leu Thr Asp Ala Val Lys Leu Ala Lys Glu Ala Gly           
        275                 280                 285                       

tac aga aat gct ggt acc gct gaa ttt ttg gtc gac aac caa aac aga        912
Tyr Arg Asn Ala Gly Thr Ala Glu Phe Leu Val Asp Asn Gln Asn Arg           
    290                 295                 300                           

cac tac ttt att gaa atc aac cca aga att caa gtc gaa cat acc atc        960
His Tyr Phe Ile Glu Ile Asn Pro Arg Ile Gln Val Glu His Thr Ile           
305                 310                 315                 320           

acc gaa gaa att acc ggt atc gac att gtc gcc gca caa att caa atc       1008
Thr Glu Glu Ile Thr Gly Ile Asp Ile Val Ala Ala Gln Ile Gln Ile           
                325                 330                 335               

gca gca ggt gct tcc ttg gaa caa ttg gga cta ttg caa gat aga atc       1056
Ala Ala Gly Ala Ser Leu Glu Gln Leu Gly Leu Leu Gln Asp Arg Ile           
            340                 345                 350                   

acc acc cgt ggt ttc gct att caa tgt cgt atc act act gaa gat cct       1104
Thr Thr Arg Gly Phe Ala Ile Gln Cys Arg Ile Thr Thr Glu Asp Pro           
        355                 360                 365                       

tcc aag aac ttc cag cca gat act ggt cgt atc gat gtt tac cgt tcc       1152
Ser Lys Asn Phe Gln Pro Asp Thr Gly Arg Ile Asp Val Tyr Arg Ser           
    370                 375                 380                           

gct ggt ggt aac ggt gtc aga ttg gat ggt ggt aac gca ttc gct ggt       1200
Ala Gly Gly Asn Gly Val Arg Leu Asp Gly Gly Asn Ala Phe Ala Gly           
385                 390                 395                 400           

tcg gtc att tca cct cat tat gat tcc atg ttg gtc aaa tgt tct tgt       1248
Ser Val Ile Ser Pro His Tyr Asp Ser Met Leu Val Lys Cys Ser Cys           
                405                 410                 415               

tcc ggt tcc act tac gaa atc gtt cgt cgt aag atg ttg cgt gcc ttg       1296
Ser Gly Ser Thr Tyr Glu Ile Val Arg Arg Lys Met Leu Arg Ala Leu           
            420                 425                 430                   

atc gaa ttc aga atc aga ggt gtg aag aca aac att cca ttc ttg cta       1344
Ile Glu Phe Arg Ile Arg Gly Val Lys Thr Asn Ile Pro Phe Leu Leu           
        435                 440                 445                       

acg ttg ttg act cat cct gtg ttc aag tcc ggt gac tac tgg act acc       1392
Thr Leu Leu Thr His Pro Val Phe Lys Ser Gly Asp Tyr Trp Thr Thr           
    450                 455                 460                           

ttc atc gat gac act cca caa ttg ttc gaa atg gtt tct tct caa aac       1440
Phe Ile Asp Asp Thr Pro Gln Leu Phe Glu Met Val Ser Ser Gln Asn           
465                 470                 475                 480           

aga gca caa aaa cta ttg cac tac ttg gcc gat ctt gcc gtt aac ggt       1488
Arg Ala Gln Lys Leu Leu His Tyr Leu Ala Asp Leu Ala Val Asn Gly           
                485                 490                 495               

tca tcg atc aag ggt caa att ggt cta cca aag tta aag act cat cct       1536
Ser Ser Ile Lys Gly Gln Ile Gly Leu Pro Lys Leu Lys Thr His Pro           
            500                 505                 510                   

act atc cca cat ttg cat aag gcc gat ggc tcc att cta gat gtg tct       1584
Thr Ile Pro His Leu His Lys Ala Asp Gly Ser Ile Leu Asp Val Ser           
        515                 520                 525                       

gcc aag cct cct gcc ggg tgg aga gat gtt cta ttg caa cac ggc cca       1632
Ala Lys Pro Pro Ala Gly Trp Arg Asp Val Leu Leu Gln His Gly Pro           
    530                 535                 540                           

gaa gaa ttt gca aag caa gtt aga aag ttc aag ggt act ttg cta atg       1680
Glu Glu Phe Ala Lys Gln Val Arg Lys Phe Lys Gly Thr Leu Leu Met           
545                 550                 555                 560           

gac acc acc tgg aga gat gct cat caa tct cta ttg gcc act aga gtc       1728
Asp Thr Thr Trp Arg Asp Ala His Gln Ser Leu Leu Ala Thr Arg Val           
                565                 570                 575               

aga act tac gat ttg gct gcc atc gct cca act act gct cat gct ttg       1776
Arg Thr Tyr Asp Leu Ala Ala Ile Ala Pro Thr Thr Ala His Ala Leu           
            580                 585                 590                   

agc ggt gct ttc gct ttg gaa tgt tgg ggt ggt gcc act ttc gat gtc       1824
Ser Gly Ala Phe Ala Leu Glu Cys Trp Gly Gly Ala Thr Phe Asp Val           
        595                 600                 605                       

tcc atg aga ttc ttg cac gaa gat cca tgg gaa cgt ttg aga act ttg       1872
Ser Met Arg Phe Leu His Glu Asp Pro Trp Glu Arg Leu Arg Thr Leu           
    610                 615                 620                           

aga aag ttg gtt cct aac att cca ttc caa atg ttg cta cgt ggt gcc       1920
Arg Lys Leu Val Pro Asn Ile Pro Phe Gln Met Leu Leu Arg Gly Ala           
625                 630                 635                 640           

aac ggt gtt gca tac tct tct cta cca gat aac gct atc gac cac ttt       1968
Asn Gly Val Ala Tyr Ser Ser Leu Pro Asp Asn Ala Ile Asp His Phe           
                645                 650                 655               

gtc aag caa gca aag gat aac ggt gtt gac att ttc aga gtc ttc gat       2016
Val Lys Gln Ala Lys Asp Asn Gly Val Asp Ile Phe Arg Val Phe Asp           
            660                 665                 670                   

gct cta aac gat ttg gag caa ttg act gtc ggt gtt gac gct gtc aag       2064
Ala Leu Asn Asp Leu Glu Gln Leu Thr Val Gly Val Asp Ala Val Lys           
        675                 680                 685                       

aag gct ggt ggt gtt gtc gaa gct acc att tgt tac tcc ggt gac atg       2112
Lys Ala Gly Gly Val Val Glu Ala Thr Ile Cys Tyr Ser Gly Asp Met           
    690                 695                 700                           

cta gca cca ggt aag aag tac aac ctt gac tac tac ttg gac att gtt       2160
Leu Ala Pro Gly Lys Lys Tyr Asn Leu Asp Tyr Tyr Leu Asp Ile Val           
705                 710                 715                 720           

gaa caa gtg gtt aag aga ggt acc cat att ctt ggt atc aag gat atg       2208
Glu Gln Val Val Lys Arg Gly Thr His Ile Leu Gly Ile Lys Asp Met           
                725                 730                 735               

gca ggt act ttg aag cca tct gct gct aag ctc ttg atc ggt tct atc       2256
Ala Gly Thr Leu Lys Pro Ser Ala Ala Lys Leu Leu Ile Gly Ser Ile           
            740                 745                 750                   

aga aca aag tac cct gac ttg cca att cac gtc cat acc cat gac tcc       2304
Arg Thr Lys Tyr Pro Asp Leu Pro Ile His Val His Thr His Asp Ser           
        755                 760                 765                       

gcc ggt acc ggt gtt gct tcc atg gct gca tgt gct ttc gct ggt gct       2352
Ala Gly Thr Gly Val Ala Ser Met Ala Ala Cys Ala Phe Ala Gly Ala           
    770                 775                 780                           

gat gtt gtt gat gtt gca acc aac tct atg tct ggt atg act tct caa       2400
Asp Val Val Asp Val Ala Thr Asn Ser Met Ser Gly Met Thr Ser Gln           
785                 790                 795                 800           

cca tct gtc aat gca cta ttg gct gct ctt gat ggt gaa atc gac tgt       2448
Pro Ser Val Asn Ala Leu Leu Ala Ala Leu Asp Gly Glu Ile Asp Cys           
                805                 810                 815               

aat gtc aac gtc agc tac atc agt cag cta gat gct tac tgg gct gaa       2496
Asn Val Asn Val Ser Tyr Ile Ser Gln Leu Asp Ala Tyr Trp Ala Glu           
            820                 825                 830                   

atg aga cta ttg tac tca tgt ttc gaa gcc gac ttg aag ggt cct gat       2544
Met Arg Leu Leu Tyr Ser Cys Phe Glu Ala Asp Leu Lys Gly Pro Asp           
        835                 840                 845                       

cca gaa gtt tac gtc cat gaa att cca ggt ggt caa ttg acc aac ttg       2592
Pro Glu Val Tyr Val His Glu Ile Pro Gly Gly Gln Leu Thr Asn Leu           
    850                 855                 860                           

ctc ttc caa gcc caa caa ttg ggt ctt ggt gag caa tgg gct gaa acc       2640
Leu Phe Gln Ala Gln Gln Leu Gly Leu Gly Glu Gln Trp Ala Glu Thr           
865                 870                 875                 880           

aag aga gct tac cgt gaa gca aac ctg ttg ttg ggt gat gtt gtt aag       2688
Lys Arg Ala Tyr Arg Glu Ala Asn Leu Leu Leu Gly Asp Val Val Lys           
                885                 890                 895               

gtc act cca aca tcc aag gtt gtc ggt gat ttg gct caa ttc atg gtc       2736
Val Thr Pro Thr Ser Lys Val Val Gly Asp Leu Ala Gln Phe Met Val           
            900                 905                 910                   

act aac aag ttg acc tcg gat gat gtt aag aga tta gct tca tct ttg       2784
Thr Asn Lys Leu Thr Ser Asp Asp Val Lys Arg Leu Ala Ser Ser Leu           
        915                 920                 925                       

gat ttc cca gac tcc gtc atg gac ttc ttt gaa ggt tta atc ggt caa       2832
Asp Phe Pro Asp Ser Val Met Asp Phe Phe Glu Gly Leu Ile Gly Gln           
    930                 935                 940                           

cca tac ggt ggt ttc cca gaa cct cta aga tct gat gtt ttg aag aac       2880
Pro Tyr Gly Gly Phe Pro Glu Pro Leu Arg Ser Asp Val Leu Lys Asn           
945                 950                 955                 960           

aag aga aga aag ttg acc aag aga cca ggt ttg gaa ttg gct cca ttc       2928
Lys Arg Arg Lys Leu Thr Lys Arg Pro Gly Leu Glu Leu Ala Pro Phe           
                965                 970                 975               

gat ttg gaa ggc att aag gaa gat ttg act aac aga ttt ggt gac att       2976
Asp Leu Glu Gly Ile Lys Glu Asp Leu Thr Asn Arg Phe Gly Asp Ile           
            980                 985                 990                   

gac gac tgt gat gtt gct tct tac  aac atg tat cca aag  gtc tac gaa     3024
Asp Asp Cys Asp Val Ala Ser Tyr  Asn Met Tyr Pro Lys  Val Tyr Glu         
        995                 1000                 1005                     

gat ttc  cgt aag atc aga gaa  aag tac ggt gat cta  tct gtt ttg        3069
Asp Phe  Arg Lys Ile Arg Glu  Lys Tyr Gly Asp Leu  Ser Val Leu            
    1010                 1015                 1020                        

cca acc  aag aac ttc ttg tct  cca cct tca atc ggt  gaa gaa atc        3114
Pro Thr  Lys Asn Phe Leu Ser  Pro Pro Ser Ile Gly  Glu Glu Ile            
    1025                 1030                 1035                        

gtc gtt  aca att gaa caa ggt  aag act ttg atc att  aag cca caa        3159
Val Val  Thr Ile Glu Gln Gly  Lys Thr Leu Ile Ile  Lys Pro Gln            
    1040                 1045                 1050                        

gct att  ggt gat ttg aac aag  gag act ggt atc aga  gaa gtt tac        3204
Ala Ile  Gly Asp Leu Asn Lys  Glu Thr Gly Ile Arg  Glu Val Tyr            
    1055                 1060                 1065                        

ttc gaa  ttg aac ggt gaa ttg  aga aag gtc tct gtt  gct gac aga        3249
Phe Glu  Leu Asn Gly Glu Leu  Arg Lys Val Ser Val  Ala Asp Arg            
    1070                 1075                 1080                        

tct caa  aag gtt gaa acg atc  tcc aag cca aag gct  gac gcc cac        3294
Ser Gln  Lys Val Glu Thr Ile  Ser Lys Pro Lys Ala  Asp Ala His            
    1085                 1090                 1095                        

gat cca  ttc caa gtt ggt tct  cca atg gca ggt gtt  gtt gtc gaa        3339
Asp Pro  Phe Gln Val Gly Ser  Pro Met Ala Gly Val  Val Val Glu            
    1100                 1105                 1110                        

gtc aag  gta cac aag ggt tct  ttg atc tcc aag ggc  caa cca gtc        3384
Val Lys  Val His Lys Gly Ser  Leu Ile Ser Lys Gly  Gln Pro Val            
    1115                 1120                 1125                        

gct gtc  cta agt gcc atg aag  atg gaa atg gtt atc  tcc tcc cca        3429
Ala Val  Leu Ser Ala Met Lys  Met Glu Met Val Ile  Ser Ser Pro            
    1130                 1135                 1140                        

tct gat  ggt caa gtc aag gaa  gtg ctt gtc aag gat  ggt gaa aac        3474
Ser Asp  Gly Gln Val Lys Glu  Val Leu Val Lys Asp  Gly Glu Asn            
    1145                 1150                 1155                        

gtt gac  gct tct gac ttg ctc  gtt gtt ttg gaa gaa  gct cca gct        3519
Val Asp  Ala Ser Asp Leu Leu  Val Val Leu Glu Glu  Ala Pro Ala            
    1160                 1165                 1170                        

aaa gaa  taa                                                          3528
Lys Glu                                                                   
    1175                                                                  


<210>  12
<211>  1175
<212>  PRT
<213>  Kluyveromyces marxianus

<400>  12

Met Ser Thr Gln Asn Asp Leu Ala Gly Leu Arg Asp Asn Ser Asn Leu 
1               5                   10                  15      


Leu Gly Glu Lys Asn Lys Ile Leu Val Ala Asn Arg Gly Glu Ile Pro 
            20                  25                  30          


Ile Arg Ile Phe Arg Thr Ala His Glu Leu Ser Met Lys Thr Val Ala 
        35                  40                  45              


Ile Tyr Ser His Glu Asp Arg Leu Ser Met His Arg Leu Lys Ala Asp 
    50                  55                  60                  


Glu Ala Tyr Val Ile Gly Glu Pro Gly Lys Tyr Thr Pro Val Gly Ala 
65                  70                  75                  80  


Tyr Leu Ala Ile Asp Glu Ile Ile Lys Ile Ala Gln Leu His Gly Val 
                85                  90                  95      


Ser Phe Ile His Pro Gly Tyr Gly Phe Leu Ser Glu Asn Ser Glu Phe 
            100                 105                 110         


Ala Lys Lys Val Ala Asp Ser Gly Ile Thr Trp Val Gly Pro Pro Ala 
        115                 120                 125             


Asp Val Ile Asp Ala Val Gly Asp Lys Val Ser Ala Arg Asn Leu Ala 
    130                 135                 140                 


Glu Arg Ala Asp Val Pro Val Val Pro Gly Thr Pro Gly Pro Ile Glu 
145                 150                 155                 160 


Thr Val Glu Glu Ala Val Glu Phe Val Glu Lys Tyr Gly Tyr Pro Val 
                165                 170                 175     


Ile Ile Lys Ala Ala Phe Gly Gly Gly Gly Arg Gly Met Arg Val Val 
            180                 185                 190         


Arg Glu Gly Asp Asp Ile Ala Asp Ala Phe Gln Arg Ala Lys Ser Glu 
        195                 200                 205             


Ala Val Thr Ala Phe Gly Asn Gly Thr Cys Phe Val Glu Arg Phe Leu 
    210                 215                 220                 


Asp Lys Pro Lys His Ile Glu Val Gln Leu Leu Ala Asp His Tyr Gly 
225                 230                 235                 240 


Asn Val Ile His Leu Phe Glu Arg Asp Cys Ser Val Gln Arg Arg His 
                245                 250                 255     


Gln Lys Val Val Glu Val Ala Pro Ala Lys Thr Leu Pro Glu Ser Val 
            260                 265                 270         


Arg Asn Ala Ile Leu Thr Asp Ala Val Lys Leu Ala Lys Glu Ala Gly 
        275                 280                 285             


Tyr Arg Asn Ala Gly Thr Ala Glu Phe Leu Val Asp Asn Gln Asn Arg 
    290                 295                 300                 


His Tyr Phe Ile Glu Ile Asn Pro Arg Ile Gln Val Glu His Thr Ile 
305                 310                 315                 320 


Thr Glu Glu Ile Thr Gly Ile Asp Ile Val Ala Ala Gln Ile Gln Ile 
                325                 330                 335     


Ala Ala Gly Ala Ser Leu Glu Gln Leu Gly Leu Leu Gln Asp Arg Ile 
            340                 345                 350         


Thr Thr Arg Gly Phe Ala Ile Gln Cys Arg Ile Thr Thr Glu Asp Pro 
        355                 360                 365             


Ser Lys Asn Phe Gln Pro Asp Thr Gly Arg Ile Asp Val Tyr Arg Ser 
    370                 375                 380                 


Ala Gly Gly Asn Gly Val Arg Leu Asp Gly Gly Asn Ala Phe Ala Gly 
385                 390                 395                 400 


Ser Val Ile Ser Pro His Tyr Asp Ser Met Leu Val Lys Cys Ser Cys 
                405                 410                 415     


Ser Gly Ser Thr Tyr Glu Ile Val Arg Arg Lys Met Leu Arg Ala Leu 
            420                 425                 430         


Ile Glu Phe Arg Ile Arg Gly Val Lys Thr Asn Ile Pro Phe Leu Leu 
        435                 440                 445             


Thr Leu Leu Thr His Pro Val Phe Lys Ser Gly Asp Tyr Trp Thr Thr 
    450                 455                 460                 


Phe Ile Asp Asp Thr Pro Gln Leu Phe Glu Met Val Ser Ser Gln Asn 
465                 470                 475                 480 


Arg Ala Gln Lys Leu Leu His Tyr Leu Ala Asp Leu Ala Val Asn Gly 
                485                 490                 495     


Ser Ser Ile Lys Gly Gln Ile Gly Leu Pro Lys Leu Lys Thr His Pro 
            500                 505                 510         


Thr Ile Pro His Leu His Lys Ala Asp Gly Ser Ile Leu Asp Val Ser 
        515                 520                 525             


Ala Lys Pro Pro Ala Gly Trp Arg Asp Val Leu Leu Gln His Gly Pro 
    530                 535                 540                 


Glu Glu Phe Ala Lys Gln Val Arg Lys Phe Lys Gly Thr Leu Leu Met 
545                 550                 555                 560 


Asp Thr Thr Trp Arg Asp Ala His Gln Ser Leu Leu Ala Thr Arg Val 
                565                 570                 575     


Arg Thr Tyr Asp Leu Ala Ala Ile Ala Pro Thr Thr Ala His Ala Leu 
            580                 585                 590         


Ser Gly Ala Phe Ala Leu Glu Cys Trp Gly Gly Ala Thr Phe Asp Val 
        595                 600                 605             


Ser Met Arg Phe Leu His Glu Asp Pro Trp Glu Arg Leu Arg Thr Leu 
    610                 615                 620                 


Arg Lys Leu Val Pro Asn Ile Pro Phe Gln Met Leu Leu Arg Gly Ala 
625                 630                 635                 640 


Asn Gly Val Ala Tyr Ser Ser Leu Pro Asp Asn Ala Ile Asp His Phe 
                645                 650                 655     


Val Lys Gln Ala Lys Asp Asn Gly Val Asp Ile Phe Arg Val Phe Asp 
            660                 665                 670         


Ala Leu Asn Asp Leu Glu Gln Leu Thr Val Gly Val Asp Ala Val Lys 
        675                 680                 685             


Lys Ala Gly Gly Val Val Glu Ala Thr Ile Cys Tyr Ser Gly Asp Met 
    690                 695                 700                 


Leu Ala Pro Gly Lys Lys Tyr Asn Leu Asp Tyr Tyr Leu Asp Ile Val 
705                 710                 715                 720 


Glu Gln Val Val Lys Arg Gly Thr His Ile Leu Gly Ile Lys Asp Met 
                725                 730                 735     


Ala Gly Thr Leu Lys Pro Ser Ala Ala Lys Leu Leu Ile Gly Ser Ile 
            740                 745                 750         


Arg Thr Lys Tyr Pro Asp Leu Pro Ile His Val His Thr His Asp Ser 
        755                 760                 765             


Ala Gly Thr Gly Val Ala Ser Met Ala Ala Cys Ala Phe Ala Gly Ala 
    770                 775                 780                 


Asp Val Val Asp Val Ala Thr Asn Ser Met Ser Gly Met Thr Ser Gln 
785                 790                 795                 800 


Pro Ser Val Asn Ala Leu Leu Ala Ala Leu Asp Gly Glu Ile Asp Cys 
                805                 810                 815     


Asn Val Asn Val Ser Tyr Ile Ser Gln Leu Asp Ala Tyr Trp Ala Glu 
            820                 825                 830         


Met Arg Leu Leu Tyr Ser Cys Phe Glu Ala Asp Leu Lys Gly Pro Asp 
        835                 840                 845             


Pro Glu Val Tyr Val His Glu Ile Pro Gly Gly Gln Leu Thr Asn Leu 
    850                 855                 860                 


Leu Phe Gln Ala Gln Gln Leu Gly Leu Gly Glu Gln Trp Ala Glu Thr 
865                 870                 875                 880 


Lys Arg Ala Tyr Arg Glu Ala Asn Leu Leu Leu Gly Asp Val Val Lys 
                885                 890                 895     


Val Thr Pro Thr Ser Lys Val Val Gly Asp Leu Ala Gln Phe Met Val 
            900                 905                 910         


Thr Asn Lys Leu Thr Ser Asp Asp Val Lys Arg Leu Ala Ser Ser Leu 
        915                 920                 925             


Asp Phe Pro Asp Ser Val Met Asp Phe Phe Glu Gly Leu Ile Gly Gln 
    930                 935                 940                 


Pro Tyr Gly Gly Phe Pro Glu Pro Leu Arg Ser Asp Val Leu Lys Asn 
945                 950                 955                 960 


Lys Arg Arg Lys Leu Thr Lys Arg Pro Gly Leu Glu Leu Ala Pro Phe 
                965                 970                 975     


Asp Leu Glu Gly Ile Lys Glu Asp Leu Thr Asn Arg Phe Gly Asp Ile 
            980                 985                 990         


Asp Asp Cys Asp Val Ala Ser Tyr  Asn Met Tyr Pro Lys  Val Tyr Glu 
        995                 1000                 1005             


Asp Phe  Arg Lys Ile Arg Glu  Lys Tyr Gly Asp Leu  Ser Val Leu 
    1010                 1015                 1020             


Pro Thr  Lys Asn Phe Leu Ser  Pro Pro Ser Ile Gly  Glu Glu Ile 
    1025                 1030                 1035             


Val Val  Thr Ile Glu Gln Gly  Lys Thr Leu Ile Ile  Lys Pro Gln 
    1040                 1045                 1050             


Ala Ile  Gly Asp Leu Asn Lys  Glu Thr Gly Ile Arg  Glu Val Tyr 
    1055                 1060                 1065             


Phe Glu  Leu Asn Gly Glu Leu  Arg Lys Val Ser Val  Ala Asp Arg 
    1070                 1075                 1080             


Ser Gln  Lys Val Glu Thr Ile  Ser Lys Pro Lys Ala  Asp Ala His 
    1085                 1090                 1095             


Asp Pro  Phe Gln Val Gly Ser  Pro Met Ala Gly Val  Val Val Glu 
    1100                 1105                 1110             


Val Lys  Val His Lys Gly Ser  Leu Ile Ser Lys Gly  Gln Pro Val 
    1115                 1120                 1125             


Ala Val  Leu Ser Ala Met Lys  Met Glu Met Val Ile  Ser Ser Pro 
    1130                 1135                 1140             


Ser Asp  Gly Gln Val Lys Glu  Val Leu Val Lys Asp  Gly Glu Asn 
    1145                 1150                 1155             


Val Asp  Ala Ser Asp Leu Leu  Val Val Leu Glu Glu  Ala Pro Ala 
    1160                 1165                 1170             


Lys Glu  
    1175 


<210>  13
<211>  1029
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(1029)

<400>  13
atg tcc aat gtt aaa gta gct cta cta ggt gcc gct ggt ggt atc ggc         48
Met Ser Asn Val Lys Val Ala Leu Leu Gly Ala Ala Gly Gly Ile Gly           
1               5                   10                  15                

caa cca ctt gct cta tta ctt aag ctt aat cca aac ata acc cat ttg         96
Gln Pro Leu Ala Leu Leu Leu Lys Leu Asn Pro Asn Ile Thr His Leu           
            20                  25                  30                    

gca ctc tat gac gtt gtg cat gtt cct gga gtg gct gcc gac cta cac        144
Ala Leu Tyr Asp Val Val His Val Pro Gly Val Ala Ala Asp Leu His           
        35                  40                  45                        

cat ata gac aca gat gta gtg att acc cac cat ttg aaa gat gaa gac        192
His Ile Asp Thr Asp Val Val Ile Thr His His Leu Lys Asp Glu Asp           
    50                  55                  60                            

ggt acg gcc ttg gca aac gcc ctc aag gac gct acg ttt gtt att gtc        240
Gly Thr Ala Leu Ala Asn Ala Leu Lys Asp Ala Thr Phe Val Ile Val           
65                  70                  75                  80            

ccc gcc ggt gtt ccg aga aag ccc ggc atg act aga ggt gat ttg ttc        288
Pro Ala Gly Val Pro Arg Lys Pro Gly Met Thr Arg Gly Asp Leu Phe           
                85                  90                  95                

aca att aat gcc ggt ata tgt gcc gaa ttg gct aat gct att agt ttg        336
Thr Ile Asn Ala Gly Ile Cys Ala Glu Leu Ala Asn Ala Ile Ser Leu           
            100                 105                 110                   

aac gct cct aat gca ttc acc ctt gtc att acc aat ccg gtc aac tcg        384
Asn Ala Pro Asn Ala Phe Thr Leu Val Ile Thr Asn Pro Val Asn Ser           
        115                 120                 125                       

acc gtt cct ata ttt aag gaa ata ttt gct aaa aat gaa gcc ttc aat        432
Thr Val Pro Ile Phe Lys Glu Ile Phe Ala Lys Asn Glu Ala Phe Asn           
    130                 135                 140                           

cca agg aga ctg ttt ggt gta act gct cta gat cat gtt aga tca aat        480
Pro Arg Arg Leu Phe Gly Val Thr Ala Leu Asp His Val Arg Ser Asn           
145                 150                 155                 160           

act ttt ctc tcg gaa tta att gac ggt aaa aat ccc caa cat ttt gat        528
Thr Phe Leu Ser Glu Leu Ile Asp Gly Lys Asn Pro Gln His Phe Asp           
                165                 170                 175               

gtc act gtt gtt ggc gga cac tct ggt aac tca att gtc ccc cta ttc        576
Val Thr Val Val Gly Gly His Ser Gly Asn Ser Ile Val Pro Leu Phe           
            180                 185                 190                   

tcc ctt gtt aag gct gcc gaa aat tta gac gat gaa att ata gat gcc        624
Ser Leu Val Lys Ala Ala Glu Asn Leu Asp Asp Glu Ile Ile Asp Ala           
        195                 200                 205                       

ttg att cat aga gtt caa tac ggt gga gat gaa gtt gtg gaa gca aag        672
Leu Ile His Arg Val Gln Tyr Gly Gly Asp Glu Val Val Glu Ala Lys           
    210                 215                 220                           

agc ggt gcg ggc tcg gca act ctt tca atg gct tat gcc gct aac aag        720
Ser Gly Ala Gly Ser Ala Thr Leu Ser Met Ala Tyr Ala Ala Asn Lys           
225                 230                 235                 240           

ttc ttc aat ata ttg ctt aat gga tac ttg ggt ttg aag aag aca atg        768
Phe Phe Asn Ile Leu Leu Asn Gly Tyr Leu Gly Leu Lys Lys Thr Met           
                245                 250                 255               

att tca agt tat gtc ttt tta gac gat tca atc aac ggc gtc cct caa        816
Ile Ser Ser Tyr Val Phe Leu Asp Asp Ser Ile Asn Gly Val Pro Gln           
            260                 265                 270                   

tta aag gaa aat ttg tct aaa ctt ttg aaa ggt tcc gag gtt gag tta        864
Leu Lys Glu Asn Leu Ser Lys Leu Leu Lys Gly Ser Glu Val Glu Leu           
        275                 280                 285                       

cca agt tat ttg gct gtt cca atg acc tat ggt aaa gaa ggt att gaa        912
Pro Ser Tyr Leu Ala Val Pro Met Thr Tyr Gly Lys Glu Gly Ile Glu           
    290                 295                 300                           

caa gtc ttt tac gat tgg gtg ttt gaa atg tca cca aag gaa aag gaa        960
Gln Val Phe Tyr Asp Trp Val Phe Glu Met Ser Pro Lys Glu Lys Glu           
305                 310                 315                 320           

aac ttc att aca gcg att gaa tac att gat caa aat att gaa aaa ggt       1008
Asn Phe Ile Thr Ala Ile Glu Tyr Ile Asp Gln Asn Ile Glu Lys Gly           
                325                 330                 335               

ctg aat ttt atg gta cgt taa                                           1029
Leu Asn Phe Met Val Arg                                                   
            340                                                           


<210>  14
<211>  342
<212>  PRT
<213>  Issatchenkia orientalis

<400>  14

Met Ser Asn Val Lys Val Ala Leu Leu Gly Ala Ala Gly Gly Ile Gly 
1               5                   10                  15      


Gln Pro Leu Ala Leu Leu Leu Lys Leu Asn Pro Asn Ile Thr His Leu 
            20                  25                  30          


Ala Leu Tyr Asp Val Val His Val Pro Gly Val Ala Ala Asp Leu His 
        35                  40                  45              


His Ile Asp Thr Asp Val Val Ile Thr His His Leu Lys Asp Glu Asp 
    50                  55                  60                  


Gly Thr Ala Leu Ala Asn Ala Leu Lys Asp Ala Thr Phe Val Ile Val 
65                  70                  75                  80  


Pro Ala Gly Val Pro Arg Lys Pro Gly Met Thr Arg Gly Asp Leu Phe 
                85                  90                  95      


Thr Ile Asn Ala Gly Ile Cys Ala Glu Leu Ala Asn Ala Ile Ser Leu 
            100                 105                 110         


Asn Ala Pro Asn Ala Phe Thr Leu Val Ile Thr Asn Pro Val Asn Ser 
        115                 120                 125             


Thr Val Pro Ile Phe Lys Glu Ile Phe Ala Lys Asn Glu Ala Phe Asn 
    130                 135                 140                 


Pro Arg Arg Leu Phe Gly Val Thr Ala Leu Asp His Val Arg Ser Asn 
145                 150                 155                 160 


Thr Phe Leu Ser Glu Leu Ile Asp Gly Lys Asn Pro Gln His Phe Asp 
                165                 170                 175     


Val Thr Val Val Gly Gly His Ser Gly Asn Ser Ile Val Pro Leu Phe 
            180                 185                 190         


Ser Leu Val Lys Ala Ala Glu Asn Leu Asp Asp Glu Ile Ile Asp Ala 
        195                 200                 205             


Leu Ile His Arg Val Gln Tyr Gly Gly Asp Glu Val Val Glu Ala Lys 
    210                 215                 220                 


Ser Gly Ala Gly Ser Ala Thr Leu Ser Met Ala Tyr Ala Ala Asn Lys 
225                 230                 235                 240 


Phe Phe Asn Ile Leu Leu Asn Gly Tyr Leu Gly Leu Lys Lys Thr Met 
                245                 250                 255     


Ile Ser Ser Tyr Val Phe Leu Asp Asp Ser Ile Asn Gly Val Pro Gln 
            260                 265                 270         


Leu Lys Glu Asn Leu Ser Lys Leu Leu Lys Gly Ser Glu Val Glu Leu 
        275                 280                 285             


Pro Ser Tyr Leu Ala Val Pro Met Thr Tyr Gly Lys Glu Gly Ile Glu 
    290                 295                 300                 


Gln Val Phe Tyr Asp Trp Val Phe Glu Met Ser Pro Lys Glu Lys Glu 
305                 310                 315                 320 


Asn Phe Ile Thr Ala Ile Glu Tyr Ile Asp Gln Asn Ile Glu Lys Gly 
                325                 330                 335     


Leu Asn Phe Met Val Arg 
            340         


<210>  15
<211>  1011
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(1011)

<400>  15
atg gtc aag gtg act att tta ggc gct gcc ggt gga att gga caa cca         48
Met Val Lys Val Thr Ile Leu Gly Ala Ala Gly Gly Ile Gly Gln Pro           
1               5                   10                  15                

ctc tca ttg tta ttg aga ctt aat cca tgg att gac gaa ttg gcc ttg         96
Leu Ser Leu Leu Leu Arg Leu Asn Pro Trp Ile Asp Glu Leu Ala Leu           
            20                  25                  30                    

ttt gat att gtc aat acc ccc ggc gtg agt tgt gat ttg tcg cat att        144
Phe Asp Ile Val Asn Thr Pro Gly Val Ser Cys Asp Leu Ser His Ile           
        35                  40                  45                        

cct gca tca cag gtt gtt aat ggc tat gct ccg aaa tcg aaa tca gat        192
Pro Ala Ser Gln Val Val Asn Gly Tyr Ala Pro Lys Ser Lys Ser Asp           
    50                  55                  60                            

aca gag aca atc aag act gcc ttg aaa ggt gct gat att gtt gtt att        240
Thr Glu Thr Ile Lys Thr Ala Leu Lys Gly Ala Asp Ile Val Val Ile           
65                  70                  75                  80            

cct gca gga att cca cgt aaa cct ggt atg aca aga aac gat ctc ttt        288
Pro Ala Gly Ile Pro Arg Lys Pro Gly Met Thr Arg Asn Asp Leu Phe           
                85                  90                  95                

aaa atc aat gcc gga atc gtt aag agt ttg att cat agt gca gga acc        336
Lys Ile Asn Ala Gly Ile Val Lys Ser Leu Ile His Ser Ala Gly Thr           
            100                 105                 110                   

act tgc cct gat gca ttt att tgt gtc att tcg aac cct gtc aac tcg        384
Thr Cys Pro Asp Ala Phe Ile Cys Val Ile Ser Asn Pro Val Asn Ser           
        115                 120                 125                       

aca gtt cca att gcc gtt gaa gaa cta aag cgt ttg aat gtt ttt aat        432
Thr Val Pro Ile Ala Val Glu Glu Leu Lys Arg Leu Asn Val Phe Asn           
    130                 135                 140                           

cca cat aaa gtt ttc ggt att acc aca ttg gac aat ttc aga tta gaa        480
Pro His Lys Val Phe Gly Ile Thr Thr Leu Asp Asn Phe Arg Leu Glu           
145                 150                 155                 160           

gaa ttt ctg agt gga gaa ctt ggt gga att gtc aaa cca aat gat tta        528
Glu Phe Leu Ser Gly Glu Leu Gly Gly Ile Val Lys Pro Asn Asp Leu           
                165                 170                 175               

tat ggt gat gta gtt gct ata ggt ggc cat tcg ggc gac tct ata gta        576
Tyr Gly Asp Val Val Ala Ile Gly Gly His Ser Gly Asp Ser Ile Val           
            180                 185                 190                   

ccg atc ttg aat tcg tgg aat ttg aat ttc atc aat gat gga gat tct        624
Pro Ile Leu Asn Ser Trp Asn Leu Asn Phe Ile Asn Asp Gly Asp Ser           
        195                 200                 205                       

tat aac aat ttg gtc aag agg gtc cag ttt gga ggc gat gag gtt gtc        672
Tyr Asn Asn Leu Val Lys Arg Val Gln Phe Gly Gly Asp Glu Val Val           
    210                 215                 220                           

aag gca aag gac ggg aaa ggt tcg gct aca ttg tca atg gct aca gct        720
Lys Ala Lys Asp Gly Lys Gly Ser Ala Thr Leu Ser Met Ala Thr Ala           
225                 230                 235                 240           

gca tac agg ttt gtc aac aac ctc ttg gac gcc att gtc aat aac aag        768
Ala Tyr Arg Phe Val Asn Asn Leu Leu Asp Ala Ile Val Asn Asn Lys           
                245                 250                 255               

aaa gtc aag gaa gtg gcc ttt gtg aaa atc gac caa ttg cca act aca        816
Lys Val Lys Glu Val Ala Phe Val Lys Ile Asp Gln Leu Pro Thr Thr           
            260                 265                 270                   

agg gtt cct tat ttt gtt gtt gat gaa act cag tat ttt agt cta ccc        864
Arg Val Pro Tyr Phe Val Val Asp Glu Thr Gln Tyr Phe Ser Leu Pro           
        275                 280                 285                       

att att ctc ggt aga cag ggg att gag agg gtc acg ttc cca gaa tct        912
Ile Ile Leu Gly Arg Gln Gly Ile Glu Arg Val Thr Phe Pro Glu Ser           
    290                 295                 300                           

ctg aca gag caa gag gtg aga atg aca aag cac gct gtt gct aaa gtt        960
Leu Thr Glu Gln Glu Val Arg Met Thr Lys His Ala Val Ala Lys Val           
305                 310                 315                 320           

aaa gtt gac gtt aat aaa ggc ttc aat ttt gtc cat ggc cca aaa ctg       1008
Lys Val Asp Val Asn Lys Gly Phe Asn Phe Val His Gly Pro Lys Leu           
                325                 330                 335               

taa                                                                   1011


<210>  16
<211>  336
<212>  PRT
<213>  Issatchenkia orientalis

<400>  16

Met Val Lys Val Thr Ile Leu Gly Ala Ala Gly Gly Ile Gly Gln Pro 
1               5                   10                  15      


Leu Ser Leu Leu Leu Arg Leu Asn Pro Trp Ile Asp Glu Leu Ala Leu 
            20                  25                  30          


Phe Asp Ile Val Asn Thr Pro Gly Val Ser Cys Asp Leu Ser His Ile 
        35                  40                  45              


Pro Ala Ser Gln Val Val Asn Gly Tyr Ala Pro Lys Ser Lys Ser Asp 
    50                  55                  60                  


Thr Glu Thr Ile Lys Thr Ala Leu Lys Gly Ala Asp Ile Val Val Ile 
65                  70                  75                  80  


Pro Ala Gly Ile Pro Arg Lys Pro Gly Met Thr Arg Asn Asp Leu Phe 
                85                  90                  95      


Lys Ile Asn Ala Gly Ile Val Lys Ser Leu Ile His Ser Ala Gly Thr 
            100                 105                 110         


Thr Cys Pro Asp Ala Phe Ile Cys Val Ile Ser Asn Pro Val Asn Ser 
        115                 120                 125             


Thr Val Pro Ile Ala Val Glu Glu Leu Lys Arg Leu Asn Val Phe Asn 
    130                 135                 140                 


Pro His Lys Val Phe Gly Ile Thr Thr Leu Asp Asn Phe Arg Leu Glu 
145                 150                 155                 160 


Glu Phe Leu Ser Gly Glu Leu Gly Gly Ile Val Lys Pro Asn Asp Leu 
                165                 170                 175     


Tyr Gly Asp Val Val Ala Ile Gly Gly His Ser Gly Asp Ser Ile Val 
            180                 185                 190         


Pro Ile Leu Asn Ser Trp Asn Leu Asn Phe Ile Asn Asp Gly Asp Ser 
        195                 200                 205             


Tyr Asn Asn Leu Val Lys Arg Val Gln Phe Gly Gly Asp Glu Val Val 
    210                 215                 220                 


Lys Ala Lys Asp Gly Lys Gly Ser Ala Thr Leu Ser Met Ala Thr Ala 
225                 230                 235                 240 


Ala Tyr Arg Phe Val Asn Asn Leu Leu Asp Ala Ile Val Asn Asn Lys 
                245                 250                 255     


Lys Val Lys Glu Val Ala Phe Val Lys Ile Asp Gln Leu Pro Thr Thr 
            260                 265                 270         


Arg Val Pro Tyr Phe Val Val Asp Glu Thr Gln Tyr Phe Ser Leu Pro 
        275                 280                 285             


Ile Ile Leu Gly Arg Gln Gly Ile Glu Arg Val Thr Phe Pro Glu Ser 
    290                 295                 300                 


Leu Thr Glu Gln Glu Val Arg Met Thr Lys His Ala Val Ala Lys Val 
305                 310                 315                 320 


Lys Val Asp Val Asn Lys Gly Phe Asn Phe Val His Gly Pro Lys Leu 
                325                 330                 335     


<210>  17
<211>  1005
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(1005)

<400>  17
atg ttc tcc aga atc tct gct aga caa ttc tcc tcc tct gct gct tcc         48
Met Phe Ser Arg Ile Ser Ala Arg Gln Phe Ser Ser Ser Ala Ala Ser           
1               5                   10                  15                

gct tac aag gtc acc gtt tta ggt gct gca ggt ggt att ggc caa cca         96
Ala Tyr Lys Val Thr Val Leu Gly Ala Ala Gly Gly Ile Gly Gln Pro           
            20                  25                  30                    

cta tct ctt ttg atg aag ttg aac cac aag gtc acc aac tta tcc ttg        144
Leu Ser Leu Leu Met Lys Leu Asn His Lys Val Thr Asn Leu Ser Leu           
        35                  40                  45                        

tac gac ttg aga ttg ggt gct ggt gtt gcc act gac ttg tcc cac att        192
Tyr Asp Leu Arg Leu Gly Ala Gly Val Ala Thr Asp Leu Ser His Ile           
    50                  55                  60                            

cca acc aac tcc gtt gtc aag ggc tat ggt cca gaa aac aat ggt ttg        240
Pro Thr Asn Ser Val Val Lys Gly Tyr Gly Pro Glu Asn Asn Gly Leu           
65                  70                  75                  80            

aag gac gcc ttg acc ggc tcc gat gtt gtt ctt att cca gct ggt gtt        288
Lys Asp Ala Leu Thr Gly Ser Asp Val Val Leu Ile Pro Ala Gly Val           
                85                  90                  95                

cca aga aaa cca ggt atg act aga gac gat ctc ttc aac acc aat gca        336
Pro Arg Lys Pro Gly Met Thr Arg Asp Asp Leu Phe Asn Thr Asn Ala           
            100                 105                 110                   

tcg att gtc aga gac ttg gca aag gct gct gca gac cac tgt cca aac        384
Ser Ile Val Arg Asp Leu Ala Lys Ala Ala Ala Asp His Cys Pro Asn           
        115                 120                 125                       

gcc gtc ttg ttg atc att tca aac cct gtc aac tca act gtc cca att        432
Ala Val Leu Leu Ile Ile Ser Asn Pro Val Asn Ser Thr Val Pro Ile           
    130                 135                 140                           

gtt gct gag gtt ttg aaa tca aag ggc gtc tac aac cca aag aag ttg        480
Val Ala Glu Val Leu Lys Ser Lys Gly Val Tyr Asn Pro Lys Lys Leu           
145                 150                 155                 160           

ttt ggt gtc acc act ttg gac gtt ttg aga tcc tcg aga ttc ttg agt        528
Phe Gly Val Thr Thr Leu Asp Val Leu Arg Ser Ser Arg Phe Leu Ser           
                165                 170                 175               

gaa gtc gtc aac acc gac cca acc acc gaa acc gtc act gtt gtt ggt        576
Glu Val Val Asn Thr Asp Pro Thr Thr Glu Thr Val Thr Val Val Gly           
            180                 185                 190                   

ggc cac tct ggt gtc acc att gtt cct tta atc tcc caa acc aaa cac        624
Gly His Ser Gly Val Thr Ile Val Pro Leu Ile Ser Gln Thr Lys His           
        195                 200                 205                       

aag gac ttg cca aag gaa acc tac gaa gca ttg gtc cac aga atc caa        672
Lys Asp Leu Pro Lys Glu Thr Tyr Glu Ala Leu Val His Arg Ile Gln           
    210                 215                 220                           

ttc ggt ggt gat gag gtt gtc aag gcc aag gac ggt gca ggt tcc gct        720
Phe Gly Gly Asp Glu Val Val Lys Ala Lys Asp Gly Ala Gly Ser Ala           
225                 230                 235                 240           

acc ttg tcc atg gcc caa gcc ggt gca aga atg gcc tcc tcc gtc ttg        768
Thr Leu Ser Met Ala Gln Ala Gly Ala Arg Met Ala Ser Ser Val Leu           
                245                 250                 255               

aag ggt ttg gct ggt gaa gtt gac att gtc gaa cca acc ttt att gac        816
Lys Gly Leu Ala Gly Glu Val Asp Ile Val Glu Pro Thr Phe Ile Asp           
            260                 265                 270                   

tct cca ttg ttc aag tcc gaa ggt gtc gaa ttc ttc tcc tcc aga gtc        864
Ser Pro Leu Phe Lys Ser Glu Gly Val Glu Phe Phe Ser Ser Arg Val           
        275                 280                 285                       

acc ctt ggt cca gaa ggt gtc caa gaa gtc cac cca ttg ggc gtc tta        912
Thr Leu Gly Pro Glu Gly Val Gln Glu Val His Pro Leu Gly Val Leu           
    290                 295                 300                           

tct act gct gaa gaa gaa atg gtt gct act gct aag gaa acc ttg aag        960
Ser Thr Ala Glu Glu Glu Met Val Ala Thr Ala Lys Glu Thr Leu Lys           
305                 310                 315                 320           

aag aac atc caa aag ggt gtc gac ttt gtc aag gct aac cca taa           1005
Lys Asn Ile Gln Lys Gly Val Asp Phe Val Lys Ala Asn Pro                   
                325                 330                                   


<210>  18
<211>  334
<212>  PRT
<213>  Issatchenkia orientalis

<400>  18

Met Phe Ser Arg Ile Ser Ala Arg Gln Phe Ser Ser Ser Ala Ala Ser 
1               5                   10                  15      


Ala Tyr Lys Val Thr Val Leu Gly Ala Ala Gly Gly Ile Gly Gln Pro 
            20                  25                  30          


Leu Ser Leu Leu Met Lys Leu Asn His Lys Val Thr Asn Leu Ser Leu 
        35                  40                  45              


Tyr Asp Leu Arg Leu Gly Ala Gly Val Ala Thr Asp Leu Ser His Ile 
    50                  55                  60                  


Pro Thr Asn Ser Val Val Lys Gly Tyr Gly Pro Glu Asn Asn Gly Leu 
65                  70                  75                  80  


Lys Asp Ala Leu Thr Gly Ser Asp Val Val Leu Ile Pro Ala Gly Val 
                85                  90                  95      


Pro Arg Lys Pro Gly Met Thr Arg Asp Asp Leu Phe Asn Thr Asn Ala 
            100                 105                 110         


Ser Ile Val Arg Asp Leu Ala Lys Ala Ala Ala Asp His Cys Pro Asn 
        115                 120                 125             


Ala Val Leu Leu Ile Ile Ser Asn Pro Val Asn Ser Thr Val Pro Ile 
    130                 135                 140                 


Val Ala Glu Val Leu Lys Ser Lys Gly Val Tyr Asn Pro Lys Lys Leu 
145                 150                 155                 160 


Phe Gly Val Thr Thr Leu Asp Val Leu Arg Ser Ser Arg Phe Leu Ser 
                165                 170                 175     


Glu Val Val Asn Thr Asp Pro Thr Thr Glu Thr Val Thr Val Val Gly 
            180                 185                 190         


Gly His Ser Gly Val Thr Ile Val Pro Leu Ile Ser Gln Thr Lys His 
        195                 200                 205             


Lys Asp Leu Pro Lys Glu Thr Tyr Glu Ala Leu Val His Arg Ile Gln 
    210                 215                 220                 


Phe Gly Gly Asp Glu Val Val Lys Ala Lys Asp Gly Ala Gly Ser Ala 
225                 230                 235                 240 


Thr Leu Ser Met Ala Gln Ala Gly Ala Arg Met Ala Ser Ser Val Leu 
                245                 250                 255     


Lys Gly Leu Ala Gly Glu Val Asp Ile Val Glu Pro Thr Phe Ile Asp 
            260                 265                 270         


Ser Pro Leu Phe Lys Ser Glu Gly Val Glu Phe Phe Ser Ser Arg Val 
        275                 280                 285             


Thr Leu Gly Pro Glu Gly Val Gln Glu Val His Pro Leu Gly Val Leu 
    290                 295                 300                 


Ser Thr Ala Glu Glu Glu Met Val Ala Thr Ala Lys Glu Thr Leu Lys 
305                 310                 315                 320 


Lys Asn Ile Gln Lys Gly Val Asp Phe Val Lys Ala Asn Pro 
                325                 330                 


<210>  19
<211>  1017
<212>  DNA
<213>  Kluyveromyces marxianus


<220>
<221>  CDS
<222>  (1)..(1017)

<400>  19
atg ctt aga gcc cta act cgc cgt caa ttt tcc tcc act gcc ttc aac         48
Met Leu Arg Ala Leu Thr Arg Arg Gln Phe Ser Ser Thr Ala Phe Asn           
1               5                   10                  15                

cca tac aag gtc acc gtt cta ggt gct ggt ggt ggt att ggt caa cca         96
Pro Tyr Lys Val Thr Val Leu Gly Ala Gly Gly Gly Ile Gly Gln Pro           
            20                  25                  30                    

ttg tcc ttg ttg ttg aag cta aac cac aag gtc act gac ttg aga cta        144
Leu Ser Leu Leu Leu Lys Leu Asn His Lys Val Thr Asp Leu Arg Leu           
        35                  40                  45                        

tac gac ttg aag ggt gcc aag ggt gtc gct gct gac ttg tct cac atc        192
Tyr Asp Leu Lys Gly Ala Lys Gly Val Ala Ala Asp Leu Ser His Ile           
    50                  55                  60                            

cca acc aac tct acc gtt act ggt tac act cca gaa tcc aag gac tct        240
Pro Thr Asn Ser Thr Val Thr Gly Tyr Thr Pro Glu Ser Lys Asp Ser           
65                  70                  75                  80            

caa gaa gaa ttg gct gct gct ttg aag gac act gag gtt gtt ttg atc        288
Gln Glu Glu Leu Ala Ala Ala Leu Lys Asp Thr Glu Val Val Leu Ile           
                85                  90                  95                

cca gct ggt gtg cca aga aag cca ggt atg acc cgt gac gat ttg ttc        336
Pro Ala Gly Val Pro Arg Lys Pro Gly Met Thr Arg Asp Asp Leu Phe           
            100                 105                 110                   

gcc atc aat gcc ggt att gtc aga gat ttg gcc act tcc atc gcc aag        384
Ala Ile Asn Ala Gly Ile Val Arg Asp Leu Ala Thr Ser Ile Ala Lys           
        115                 120                 125                       

aac gct cca aac gcc gcc atc ttg gtc atc tcc aac cca gtc aac tct        432
Asn Ala Pro Asn Ala Ala Ile Leu Val Ile Ser Asn Pro Val Asn Ser           
    130                 135                 140                           

act gtc cca atc gtc gcc gag gtc ttg aag caa aac ggc gtc tac aac        480
Thr Val Pro Ile Val Ala Glu Val Leu Lys Gln Asn Gly Val Tyr Asn           
145                 150                 155                 160           

cca aag aag ttg ttc ggt gtc acc act ttg gac gtt atc cgt gcc tcc        528
Pro Lys Lys Leu Phe Gly Val Thr Thr Leu Asp Val Ile Arg Ala Ser           
                165                 170                 175               

aga ttc atc tcc gag gtt aga ggt acc gac cca acc act gag cac gtg        576
Arg Phe Ile Ser Glu Val Arg Gly Thr Asp Pro Thr Thr Glu His Val           
            180                 185                 190                   

acc gtc gtc ggt ggt cac tcc ggt atc acc atc ttg ccg cta gtg tcc        624
Thr Val Val Gly Gly His Ser Gly Ile Thr Ile Leu Pro Leu Val Ser           
        195                 200                 205                       

cag acc aag cac aag tcc gtc atc aag ggc gag gaa ttg gac aac ttg        672
Gln Thr Lys His Lys Ser Val Ile Lys Gly Glu Glu Leu Asp Asn Leu           
    210                 215                 220                           

atc cac aga atc caa ttc ggt ggt gac gaa gtc gtc cag gca aag aac        720
Ile His Arg Ile Gln Phe Gly Gly Asp Glu Val Val Gln Ala Lys Asn           
225                 230                 235                 240           

ggt gct ggt tct gcc act ttg tcc atg gcc caa gcc ggt gcc cgt ttc        768
Gly Ala Gly Ser Ala Thr Leu Ser Met Ala Gln Ala Gly Ala Arg Phe           
                245                 250                 255               

gct aac agc gtt cta agc ggt ttc gaa ggt gaa aga gac gtc att gag        816
Ala Asn Ser Val Leu Ser Gly Phe Glu Gly Glu Arg Asp Val Ile Glu           
            260                 265                 270                   

cca act ttc gtc gac tcc cca ttg ttc aag gac gaa ggt atc gaa ttc        864
Pro Thr Phe Val Asp Ser Pro Leu Phe Lys Asp Glu Gly Ile Glu Phe           
        275                 280                 285                       

ttc gct tcc cca gtc act ttg ggc cca gaa ggt gtc gaa aag atc cac        912
Phe Ala Ser Pro Val Thr Leu Gly Pro Glu Gly Val Glu Lys Ile His           
    290                 295                 300                           

ggt ttg ggt gtc ttg tcc gac aag gaa gaa caa atg ttg gcc act tgt        960
Gly Leu Gly Val Leu Ser Asp Lys Glu Glu Gln Met Leu Ala Thr Cys           
305                 310                 315                 320           

aag gaa acc ttg aag aag aac atc gaa aag ggt caa aac ttt gtc aag       1008
Lys Glu Thr Leu Lys Lys Asn Ile Glu Lys Gly Gln Asn Phe Val Lys           
                325                 330                 335               

caa aac taa                                                           1017
Gln Asn                                                                   
                                                                          


<210>  20
<211>  338
<212>  PRT
<213>  Kluyveromyces marxianus

<400>  20

Met Leu Arg Ala Leu Thr Arg Arg Gln Phe Ser Ser Thr Ala Phe Asn 
1               5                   10                  15      


Pro Tyr Lys Val Thr Val Leu Gly Ala Gly Gly Gly Ile Gly Gln Pro 
            20                  25                  30          


Leu Ser Leu Leu Leu Lys Leu Asn His Lys Val Thr Asp Leu Arg Leu 
        35                  40                  45              


Tyr Asp Leu Lys Gly Ala Lys Gly Val Ala Ala Asp Leu Ser His Ile 
    50                  55                  60                  


Pro Thr Asn Ser Thr Val Thr Gly Tyr Thr Pro Glu Ser Lys Asp Ser 
65                  70                  75                  80  


Gln Glu Glu Leu Ala Ala Ala Leu Lys Asp Thr Glu Val Val Leu Ile 
                85                  90                  95      


Pro Ala Gly Val Pro Arg Lys Pro Gly Met Thr Arg Asp Asp Leu Phe 
            100                 105                 110         


Ala Ile Asn Ala Gly Ile Val Arg Asp Leu Ala Thr Ser Ile Ala Lys 
        115                 120                 125             


Asn Ala Pro Asn Ala Ala Ile Leu Val Ile Ser Asn Pro Val Asn Ser 
    130                 135                 140                 


Thr Val Pro Ile Val Ala Glu Val Leu Lys Gln Asn Gly Val Tyr Asn 
145                 150                 155                 160 


Pro Lys Lys Leu Phe Gly Val Thr Thr Leu Asp Val Ile Arg Ala Ser 
                165                 170                 175     


Arg Phe Ile Ser Glu Val Arg Gly Thr Asp Pro Thr Thr Glu His Val 
            180                 185                 190         


Thr Val Val Gly Gly His Ser Gly Ile Thr Ile Leu Pro Leu Val Ser 
        195                 200                 205             


Gln Thr Lys His Lys Ser Val Ile Lys Gly Glu Glu Leu Asp Asn Leu 
    210                 215                 220                 


Ile His Arg Ile Gln Phe Gly Gly Asp Glu Val Val Gln Ala Lys Asn 
225                 230                 235                 240 


Gly Ala Gly Ser Ala Thr Leu Ser Met Ala Gln Ala Gly Ala Arg Phe 
                245                 250                 255     


Ala Asn Ser Val Leu Ser Gly Phe Glu Gly Glu Arg Asp Val Ile Glu 
            260                 265                 270         


Pro Thr Phe Val Asp Ser Pro Leu Phe Lys Asp Glu Gly Ile Glu Phe 
        275                 280                 285             


Phe Ala Ser Pro Val Thr Leu Gly Pro Glu Gly Val Glu Lys Ile His 
    290                 295                 300                 


Gly Leu Gly Val Leu Ser Asp Lys Glu Glu Gln Met Leu Ala Thr Cys 
305                 310                 315                 320 


Lys Glu Thr Leu Lys Lys Asn Ile Glu Lys Gly Gln Asn Phe Val Lys 
                325                 330                 335     


Gln Asn 
        


<210>  21
<211>  1017
<212>  DNA
<213>  Kluyveromyces marxianus


<220>
<221>  CDS
<222>  (1)..(1017)

<400>  21
atg gtt agc gtt gca gta tta gga tca tcc gga ggc att ggc caa cca         48
Met Val Ser Val Ala Val Leu Gly Ser Ser Gly Gly Ile Gly Gln Pro           
1               5                   10                  15                

ctc tca ctc ttg ttg aag ctg gac cct cgc gtg tcc agc ttg aga ttg         96
Leu Ser Leu Leu Leu Lys Leu Asp Pro Arg Val Ser Ser Leu Arg Leu           
            20                  25                  30                    

tac gac ttg aag atg tcc cac ggg atc gcc acc gat ttg tcg cac atg        144
Tyr Asp Leu Lys Met Ser His Gly Ile Ala Thr Asp Leu Ser His Met           
        35                  40                  45                        

gac tcc aac tcc atc tgc gag ggc ttc aac acc gac gag atc gcg ctc        192
Asp Ser Asn Ser Ile Cys Glu Gly Phe Asn Thr Asp Glu Ile Ala Leu           
    50                  55                  60                            

gcg ctc aag ggc gcc cag atc gtc gtc atc ccc gcg ggt gtc cca aga        240
Ala Leu Lys Gly Ala Gln Ile Val Val Ile Pro Ala Gly Val Pro Arg           
65                  70                  75                  80            

aag ccc ggg atg tca cgt gac gac ctt ttc aag atc aac gcc aag atc        288
Lys Pro Gly Met Ser Arg Asp Asp Leu Phe Lys Ile Asn Ala Lys Ile           
                85                  90                  95                

atc aag tcg ttg gcg ttg caa ata gcc gag cac gcg ccc gag gcg cgc        336
Ile Lys Ser Leu Ala Leu Gln Ile Ala Glu His Ala Pro Glu Ala Arg           
            100                 105                 110                   

gtc ctc gtg atc tcg aac ccg gtc aac tcc ttg gtg ccc att gtg tac        384
Val Leu Val Ile Ser Asn Pro Val Asn Ser Leu Val Pro Ile Val Tyr           
        115                 120                 125                       

gag act ttg aag agc gtc ggc aag ttc gag ccg ggt aaa gtg atg gga        432
Glu Thr Leu Lys Ser Val Gly Lys Phe Glu Pro Gly Lys Val Met Gly           
    130                 135                 140                           

att acc aca ttg gac att atc cgc tca cac acg ttc ctg gtg gac gtc        480
Ile Thr Thr Leu Asp Ile Ile Arg Ser His Thr Phe Leu Val Asp Val           
145                 150                 155                 160           

ttg ggc cgc aag gcg tac agc gtc gag aag ttg cgc agc gcg gtt act        528
Leu Gly Arg Lys Ala Tyr Ser Val Glu Lys Leu Arg Ser Ala Val Thr           
                165                 170                 175               

gtg gtg ggc ggc cac tcg ggc gag acc att gtt ccg att ttc acc gac        576
Val Val Gly Gly His Ser Gly Glu Thr Ile Val Pro Ile Phe Thr Asp           
            180                 185                 190                   

cag aag ttc tac agg cgt ctc aga gac aga gag ctc tat gac gcg tac        624
Gln Lys Phe Tyr Arg Arg Leu Arg Asp Arg Glu Leu Tyr Asp Ala Tyr           
        195                 200                 205                       

gtg cat agg gtc caa ttc ggc gga gac gag gtc gtg aag gcc aag gac        672
Val His Arg Val Gln Phe Gly Gly Asp Glu Val Val Lys Ala Lys Asp           
    210                 215                 220                           

ggc agc ggt agt gct act ttg tct atg gcc tgg gcg ggt tac agt ttt        720
Gly Ser Gly Ser Ala Thr Leu Ser Met Ala Trp Ala Gly Tyr Ser Phe           
225                 230                 235                 240           

gtg aag cag ttg ctc aac agc ttg cac cta gaa aca ggc gaa gac gtg        768
Val Lys Gln Leu Leu Asn Ser Leu His Leu Glu Thr Gly Glu Asp Val           
                245                 250                 255               

cat ccg atc cca acg ttt gtg tac ttg ccg ggt tta ccg ggc ggg aag        816
His Pro Ile Pro Thr Phe Val Tyr Leu Pro Gly Leu Pro Gly Gly Lys           
            260                 265                 270                   

gag ctc cag cag aag ttg ggc acc tct gtt gag ttt ttt gcc gcg ccc        864
Glu Leu Gln Gln Lys Leu Gly Thr Ser Val Glu Phe Phe Ala Ala Pro           
        275                 280                 285                       

gtg aag ctt tcc aag ggt att gtg gtt gaa gtt gag cac gac tgg gtc        912
Val Lys Leu Ser Lys Gly Ile Val Val Glu Val Glu His Asp Trp Val           
    290                 295                 300                           

gac aag ttg aac gat gcc gag aag aag ttg att gca aag tgt ctt cca        960
Asp Lys Leu Asn Asp Ala Glu Lys Lys Leu Ile Ala Lys Cys Leu Pro           
305                 310                 315                 320           

atc ctt gac aag aac atc aag aag ggt ctc gcc ttt tcg cag cag aca       1008
Ile Leu Asp Lys Asn Ile Lys Lys Gly Leu Ala Phe Ser Gln Gln Thr           
                325                 330                 335               

aag ttg tga                                                           1017
Lys Leu                                                                   
                                                                          


<210>  22
<211>  338
<212>  PRT
<213>  Kluyveromyces marxianus

<400>  22

Met Val Ser Val Ala Val Leu Gly Ser Ser Gly Gly Ile Gly Gln Pro 
1               5                   10                  15      


Leu Ser Leu Leu Leu Lys Leu Asp Pro Arg Val Ser Ser Leu Arg Leu 
            20                  25                  30          


Tyr Asp Leu Lys Met Ser His Gly Ile Ala Thr Asp Leu Ser His Met 
        35                  40                  45              


Asp Ser Asn Ser Ile Cys Glu Gly Phe Asn Thr Asp Glu Ile Ala Leu 
    50                  55                  60                  


Ala Leu Lys Gly Ala Gln Ile Val Val Ile Pro Ala Gly Val Pro Arg 
65                  70                  75                  80  


Lys Pro Gly Met Ser Arg Asp Asp Leu Phe Lys Ile Asn Ala Lys Ile 
                85                  90                  95      


Ile Lys Ser Leu Ala Leu Gln Ile Ala Glu His Ala Pro Glu Ala Arg 
            100                 105                 110         


Val Leu Val Ile Ser Asn Pro Val Asn Ser Leu Val Pro Ile Val Tyr 
        115                 120                 125             


Glu Thr Leu Lys Ser Val Gly Lys Phe Glu Pro Gly Lys Val Met Gly 
    130                 135                 140                 


Ile Thr Thr Leu Asp Ile Ile Arg Ser His Thr Phe Leu Val Asp Val 
145                 150                 155                 160 


Leu Gly Arg Lys Ala Tyr Ser Val Glu Lys Leu Arg Ser Ala Val Thr 
                165                 170                 175     


Val Val Gly Gly His Ser Gly Glu Thr Ile Val Pro Ile Phe Thr Asp 
            180                 185                 190         


Gln Lys Phe Tyr Arg Arg Leu Arg Asp Arg Glu Leu Tyr Asp Ala Tyr 
        195                 200                 205             


Val His Arg Val Gln Phe Gly Gly Asp Glu Val Val Lys Ala Lys Asp 
    210                 215                 220                 


Gly Ser Gly Ser Ala Thr Leu Ser Met Ala Trp Ala Gly Tyr Ser Phe 
225                 230                 235                 240 


Val Lys Gln Leu Leu Asn Ser Leu His Leu Glu Thr Gly Glu Asp Val 
                245                 250                 255     


His Pro Ile Pro Thr Phe Val Tyr Leu Pro Gly Leu Pro Gly Gly Lys 
            260                 265                 270         


Glu Leu Gln Gln Lys Leu Gly Thr Ser Val Glu Phe Phe Ala Ala Pro 
        275                 280                 285             


Val Lys Leu Ser Lys Gly Ile Val Val Glu Val Glu His Asp Trp Val 
    290                 295                 300                 


Asp Lys Leu Asn Asp Ala Glu Lys Lys Leu Ile Ala Lys Cys Leu Pro 
305                 310                 315                 320 


Ile Leu Asp Lys Asn Ile Lys Lys Gly Leu Ala Phe Ser Gln Gln Thr 
                325                 330                 335     


Lys Leu 
        


<210>  23
<211>  1125
<212>  DNA
<213>  Kluyveromyces marxianus


<220>
<221>  CDS
<222>  (1)..(1125)

<400>  23
atg cca gca gta tca tat gat gtc cag caa cgg gat atc ctc aag atc         48
Met Pro Ala Val Ser Tyr Asp Val Gln Gln Arg Asp Ile Leu Lys Ile           
1               5                   10                  15                

gca gtt cta ggg gcg gca ggc ggt att ggc caa tcc ttg tcg ctc ttg         96
Ala Val Leu Gly Ala Ala Gly Gly Ile Gly Gln Ser Leu Ser Leu Leu           
            20                  25                  30                    

ttg aag tcg aac gct tct ttt ttg tta cca cgt gac tcg tca aga cac        144
Leu Lys Ser Asn Ala Ser Phe Leu Leu Pro Arg Asp Ser Ser Arg His           
        35                  40                  45                        

ata agc cta gcg cta tac gac gtg aac aaa gat gcc atc gtg ggc aca        192
Ile Ser Leu Ala Leu Tyr Asp Val Asn Lys Asp Ala Ile Val Gly Thr           
    50                  55                  60                            

gca gca gac ttg tca cac ata gac acc cct atc acc acc act cca cac        240
Ala Ala Asp Leu Ser His Ile Asp Thr Pro Ile Thr Thr Thr Pro His           
65                  70                  75                  80            

tac cca aat gat ggg aat ggc ggt atc gca cgg tgc ttg caa gat gca        288
Tyr Pro Asn Asp Gly Asn Gly Gly Ile Ala Arg Cys Leu Gln Asp Ala           
                85                  90                  95                

gac atg gtc atc atc cca gca ggt gtg ccc aga aaa ccc ggt atg tca        336
Asp Met Val Ile Ile Pro Ala Gly Val Pro Arg Lys Pro Gly Met Ser           
            100                 105                 110                   

cgt gat gac cta atc ggt gtc aac gcc aag atc atc aag tcg cta gga        384
Arg Asp Asp Leu Ile Gly Val Asn Ala Lys Ile Ile Lys Ser Leu Gly           
        115                 120                 125                       

aac gac atc gca gag tac tgt gac ttg tct aaa gtg cat gta ttg gtt        432
Asn Asp Ile Ala Glu Tyr Cys Asp Leu Ser Lys Val His Val Leu Val           
    130                 135                 140                           

att tcg aac cca gtg aac tcg ttg gtc cca ctg atg gtg tcg act ttg        480
Ile Ser Asn Pro Val Asn Ser Leu Val Pro Leu Met Val Ser Thr Leu           
145                 150                 155                 160           

gca aac agc cca cac agt gcg aac aca aac atc gag gct aga gtg tac        528
Ala Asn Ser Pro His Ser Ala Asn Thr Asn Ile Glu Ala Arg Val Tyr           
                165                 170                 175               

ggg atc acc cat ttg gac cta gtg aga gct tcc acc ttt gtg caa cag        576
Gly Ile Thr His Leu Asp Leu Val Arg Ala Ser Thr Phe Val Gln Gln           
            180                 185                 190                   

cta aac tct ttc aaa tca aat aac gca cct gac att ccg gtc att ggt        624
Leu Asn Ser Phe Lys Ser Asn Asn Ala Pro Asp Ile Pro Val Ile Gly           
        195                 200                 205                       

ggt cat tcc gga gat acc atc atc ccc gtt ttt tcc gtc ttg aat cac        672
Gly His Ser Gly Asp Thr Ile Ile Pro Val Phe Ser Val Leu Asn His           
    210                 215                 220                           

cgc gct tct aac tcc gga tac gct aat ttg cta gat aat ggc gtt agg        720
Arg Ala Ser Asn Ser Gly Tyr Ala Asn Leu Leu Asp Asn Gly Val Arg           
225                 230                 235                 240           

caa aag ttg gtc cac aga gtt caa tat ggt ggg gac gaa atc gtc caa        768
Gln Lys Leu Val His Arg Val Gln Tyr Gly Gly Asp Glu Ile Val Gln           
                245                 250                 255               

gca aag aac ggt aac ggg agc gcg aca tta tcc atg gca tac gcg ggc        816
Ala Lys Asn Gly Asn Gly Ser Ala Thr Leu Ser Met Ala Tyr Ala Gly           
            260                 265                 270                   

ttc aaa atc gca gca caa ttc atc gac ctt ttg gtc gga aat atc cgc        864
Phe Lys Ile Ala Ala Gln Phe Ile Asp Leu Leu Val Gly Asn Ile Arg           
        275                 280                 285                       

act atc gaa aat att tgc atg tat gtt ccg ctc act aac agg tat aat        912
Thr Ile Glu Asn Ile Cys Met Tyr Val Pro Leu Thr Asn Arg Tyr Asn           
    290                 295                 300                           

acc gag atc gcc cca ggc tct gac gaa tta aga tca aag tac atc aac        960
Thr Glu Ile Ala Pro Gly Ser Asp Glu Leu Arg Ser Lys Tyr Ile Asn           
305                 310                 315                 320           

gga acc ctt tat ttc tcg att cca ctt tcc atc gga ata aac ggt atc       1008
Gly Thr Leu Tyr Phe Ser Ile Pro Leu Ser Ile Gly Ile Asn Gly Ile           
                325                 330                 335               

gaa aga gtc cac tac gag atc atg gaa cat cta gac agc tac gag cgt       1056
Glu Arg Val His Tyr Glu Ile Met Glu His Leu Asp Ser Tyr Glu Arg           
            340                 345                 350                   

gag acg cta cta ccg atc tgc ttg gaa act cta aag ggt aat att gac       1104
Glu Thr Leu Leu Pro Ile Cys Leu Glu Thr Leu Lys Gly Asn Ile Asp           
        355                 360                 365                       

aag ggt cta agc ttg gta taa                                           1125
Lys Gly Leu Ser Leu Val                                                   
    370                                                                   


<210>  24
<211>  374
<212>  PRT
<213>  Kluyveromyces marxianus

<400>  24

Met Pro Ala Val Ser Tyr Asp Val Gln Gln Arg Asp Ile Leu Lys Ile 
1               5                   10                  15      


Ala Val Leu Gly Ala Ala Gly Gly Ile Gly Gln Ser Leu Ser Leu Leu 
            20                  25                  30          


Leu Lys Ser Asn Ala Ser Phe Leu Leu Pro Arg Asp Ser Ser Arg His 
        35                  40                  45              


Ile Ser Leu Ala Leu Tyr Asp Val Asn Lys Asp Ala Ile Val Gly Thr 
    50                  55                  60                  


Ala Ala Asp Leu Ser His Ile Asp Thr Pro Ile Thr Thr Thr Pro His 
65                  70                  75                  80  


Tyr Pro Asn Asp Gly Asn Gly Gly Ile Ala Arg Cys Leu Gln Asp Ala 
                85                  90                  95      


Asp Met Val Ile Ile Pro Ala Gly Val Pro Arg Lys Pro Gly Met Ser 
            100                 105                 110         


Arg Asp Asp Leu Ile Gly Val Asn Ala Lys Ile Ile Lys Ser Leu Gly 
        115                 120                 125             


Asn Asp Ile Ala Glu Tyr Cys Asp Leu Ser Lys Val His Val Leu Val 
    130                 135                 140                 


Ile Ser Asn Pro Val Asn Ser Leu Val Pro Leu Met Val Ser Thr Leu 
145                 150                 155                 160 


Ala Asn Ser Pro His Ser Ala Asn Thr Asn Ile Glu Ala Arg Val Tyr 
                165                 170                 175     


Gly Ile Thr His Leu Asp Leu Val Arg Ala Ser Thr Phe Val Gln Gln 
            180                 185                 190         


Leu Asn Ser Phe Lys Ser Asn Asn Ala Pro Asp Ile Pro Val Ile Gly 
        195                 200                 205             


Gly His Ser Gly Asp Thr Ile Ile Pro Val Phe Ser Val Leu Asn His 
    210                 215                 220                 


Arg Ala Ser Asn Ser Gly Tyr Ala Asn Leu Leu Asp Asn Gly Val Arg 
225                 230                 235                 240 


Gln Lys Leu Val His Arg Val Gln Tyr Gly Gly Asp Glu Ile Val Gln 
                245                 250                 255     


Ala Lys Asn Gly Asn Gly Ser Ala Thr Leu Ser Met Ala Tyr Ala Gly 
            260                 265                 270         


Phe Lys Ile Ala Ala Gln Phe Ile Asp Leu Leu Val Gly Asn Ile Arg 
        275                 280                 285             


Thr Ile Glu Asn Ile Cys Met Tyr Val Pro Leu Thr Asn Arg Tyr Asn 
    290                 295                 300                 


Thr Glu Ile Ala Pro Gly Ser Asp Glu Leu Arg Ser Lys Tyr Ile Asn 
305                 310                 315                 320 


Gly Thr Leu Tyr Phe Ser Ile Pro Leu Ser Ile Gly Ile Asn Gly Ile 
                325                 330                 335     


Glu Arg Val His Tyr Glu Ile Met Glu His Leu Asp Ser Tyr Glu Arg 
            340                 345                 350         


Glu Thr Leu Leu Pro Ile Cys Leu Glu Thr Leu Lys Gly Asn Ile Asp 
        355                 360                 365             


Lys Gly Leu Ser Leu Val 
    370                 


<210>  25
<211>  1071
<212>  DNA
<213>  Zygosaccharomyces rouxii


<220>
<221>  CDS
<222>  (1)..(1071)

<400>  25
atg cct cat tct atc aac ggt gat gtt aaa atc gca gta ttg gga gct         48
Met Pro His Ser Ile Asn Gly Asp Val Lys Ile Ala Val Leu Gly Ala           
1               5                   10                  15                

gca ggt ggt att gga caa tca ctt tcg cta ctt ttg aag acc cag tta         96
Ala Gly Gly Ile Gly Gln Ser Leu Ser Leu Leu Leu Lys Thr Gln Leu           
            20                  25                  30                    

act aga gaa ttg cca aat cat cgt cat gct cag tta gcc cta tac gac        144
Thr Arg Glu Leu Pro Asn His Arg His Ala Gln Leu Ala Leu Tyr Asp           
        35                  40                  45                        

gtc aat gct gac gca gtt cgg ggt gtc gca gcc gac tta tct cat att        192
Val Asn Ala Asp Ala Val Arg Gly Val Ala Ala Asp Leu Ser His Ile           
    50                  55                  60                            

gat aca ggt gtt act gta aca gga tat gaa ggt gat agg atc ggc gaa        240
Asp Thr Gly Val Thr Val Thr Gly Tyr Glu Gly Asp Arg Ile Gly Glu           
65                  70                  75                  80            

gcg tta gaa ggt acg gat atc gtc ctg atc cct gca ggt gtt cct aga        288
Ala Leu Glu Gly Thr Asp Ile Val Leu Ile Pro Ala Gly Val Pro Arg           
                85                  90                  95                

aaa cct ggt atg aca aga gaa gat cta ttg gtt gtt aat gca aag att        336
Lys Pro Gly Met Thr Arg Glu Asp Leu Leu Val Val Asn Ala Lys Ile           
            100                 105                 110                   

gtc aag agt ata ggg tca tcg att gcg cag cat tgc gat tta aac aaa        384
Val Lys Ser Ile Gly Ser Ser Ile Ala Gln His Cys Asp Leu Asn Lys           
        115                 120                 125                       

gtg ttc att cta cta atc tca aac cca ata aat tcc ctt gtt cca gta        432
Val Phe Ile Leu Leu Ile Ser Asn Pro Ile Asn Ser Leu Val Pro Val           
    130                 135                 140                           

ctc gtt aag gaa ctg gaa tct aaa tct caa ggc act caa gtt gag aga        480
Leu Val Lys Glu Leu Glu Ser Lys Ser Gln Gly Thr Gln Val Glu Arg           
145                 150                 155                 160           

cgt gtg ctt ggt ctc act aag ttg gat tcc gtt aga gca agt gca ttt        528
Arg Val Leu Gly Leu Thr Lys Leu Asp Ser Val Arg Ala Ser Ala Phe           
                165                 170                 175               

ttg cac gag gtt acg att aaa cat ggt cta aaa cct aaa tct aat act        576
Leu His Glu Val Thr Ile Lys His Gly Leu Lys Pro Lys Ser Asn Thr           
            180                 185                 190                   

ctt gat gat gtt cca gta gtt ggt ggt cat tct ggt gaa act att gta        624
Leu Asp Asp Val Pro Val Val Gly Gly His Ser Gly Glu Thr Ile Val           
        195                 200                 205                       

cct tta ttc tcc caa gcc cct aat ggt aac cgt tta tca cag gac gcc        672
Pro Leu Phe Ser Gln Ala Pro Asn Gly Asn Arg Leu Ser Gln Asp Ala           
    210                 215                 220                           

ttg gaa gct ctt gtt cag cgt gta caa ttc gga ggc gat gaa gtc gtt        720
Leu Glu Ala Leu Val Gln Arg Val Gln Phe Gly Gly Asp Glu Val Val           
225                 230                 235                 240           

aga gct aaa aat ggt gct ggt agt gcc act ctg tgt atg gcc cat gcc        768
Arg Ala Lys Asn Gly Ala Gly Ser Ala Thr Leu Cys Met Ala His Ala           
                245                 250                 255               

gct tat act gtt gct gca tct ttt att cca ctt atc act ggt caa aag        816
Ala Tyr Thr Val Ala Ala Ser Phe Ile Pro Leu Ile Thr Gly Gln Lys           
            260                 265                 270                   

cgt tcc atc tct ggt aca ttc tat gtt gcc tta aag gat gct caa ggt        864
Arg Ser Ile Ser Gly Thr Phe Tyr Val Ala Leu Lys Asp Ala Gln Gly           
        275                 280                 285                       

cag cct atc aac agt agc gct aag cgt ctt ttg ggc tca atc aac gat        912
Gln Pro Ile Asn Ser Ser Ala Lys Arg Leu Leu Gly Ser Ile Asn Asp           
    290                 295                 300                           

tta cca tat ttt gca gtg cca ttg gag att act tct cag ggt gtg gat        960
Leu Pro Tyr Phe Ala Val Pro Leu Glu Ile Thr Ser Gln Gly Val Asp           
305                 310                 315                 320           

gaa tta gat acc agc gtt ttg gaa aga atg acc aag tat gag aga gaa       1008
Glu Leu Asp Thr Ser Val Leu Glu Arg Met Thr Lys Tyr Glu Arg Glu           
                325                 330                 335               

aga ctc tta gct cct tgt ctg ggt aaa ttg gaa ggt ggt atc aga aac       1056
Arg Leu Leu Ala Pro Cys Leu Gly Lys Leu Glu Gly Gly Ile Arg Asn           
            340                 345                 350                   

ggt ttg agt ttg tga                                                   1071
Gly Leu Ser Leu                                                           
        355                                                               


<210>  26
<211>  356
<212>  PRT
<213>  Zygosaccharomyces rouxii

<400>  26

Met Pro His Ser Ile Asn Gly Asp Val Lys Ile Ala Val Leu Gly Ala 
1               5                   10                  15      


Ala Gly Gly Ile Gly Gln Ser Leu Ser Leu Leu Leu Lys Thr Gln Leu 
            20                  25                  30          


Thr Arg Glu Leu Pro Asn His Arg His Ala Gln Leu Ala Leu Tyr Asp 
        35                  40                  45              


Val Asn Ala Asp Ala Val Arg Gly Val Ala Ala Asp Leu Ser His Ile 
    50                  55                  60                  


Asp Thr Gly Val Thr Val Thr Gly Tyr Glu Gly Asp Arg Ile Gly Glu 
65                  70                  75                  80  


Ala Leu Glu Gly Thr Asp Ile Val Leu Ile Pro Ala Gly Val Pro Arg 
                85                  90                  95      


Lys Pro Gly Met Thr Arg Glu Asp Leu Leu Val Val Asn Ala Lys Ile 
            100                 105                 110         


Val Lys Ser Ile Gly Ser Ser Ile Ala Gln His Cys Asp Leu Asn Lys 
        115                 120                 125             


Val Phe Ile Leu Leu Ile Ser Asn Pro Ile Asn Ser Leu Val Pro Val 
    130                 135                 140                 


Leu Val Lys Glu Leu Glu Ser Lys Ser Gln Gly Thr Gln Val Glu Arg 
145                 150                 155                 160 


Arg Val Leu Gly Leu Thr Lys Leu Asp Ser Val Arg Ala Ser Ala Phe 
                165                 170                 175     


Leu His Glu Val Thr Ile Lys His Gly Leu Lys Pro Lys Ser Asn Thr 
            180                 185                 190         


Leu Asp Asp Val Pro Val Val Gly Gly His Ser Gly Glu Thr Ile Val 
        195                 200                 205             


Pro Leu Phe Ser Gln Ala Pro Asn Gly Asn Arg Leu Ser Gln Asp Ala 
    210                 215                 220                 


Leu Glu Ala Leu Val Gln Arg Val Gln Phe Gly Gly Asp Glu Val Val 
225                 230                 235                 240 


Arg Ala Lys Asn Gly Ala Gly Ser Ala Thr Leu Cys Met Ala His Ala 
                245                 250                 255     


Ala Tyr Thr Val Ala Ala Ser Phe Ile Pro Leu Ile Thr Gly Gln Lys 
            260                 265                 270         


Arg Ser Ile Ser Gly Thr Phe Tyr Val Ala Leu Lys Asp Ala Gln Gly 
        275                 280                 285             


Gln Pro Ile Asn Ser Ser Ala Lys Arg Leu Leu Gly Ser Ile Asn Asp 
    290                 295                 300                 


Leu Pro Tyr Phe Ala Val Pro Leu Glu Ile Thr Ser Gln Gly Val Asp 
305                 310                 315                 320 


Glu Leu Asp Thr Ser Val Leu Glu Arg Met Thr Lys Tyr Glu Arg Glu 
                325                 330                 335     


Arg Leu Leu Ala Pro Cys Leu Gly Lys Leu Glu Gly Gly Ile Arg Asn 
            340                 345                 350         


Gly Leu Ser Leu 
        355     


<210>  27
<211>  939
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(939)

<400>  27
atg aaa gtc gca gtc ctc ggc gct gct ggc ggt att ggc cag gcg ctt         48
Met Lys Val Ala Val Leu Gly Ala Ala Gly Gly Ile Gly Gln Ala Leu           
1               5                   10                  15                

gca cta ctg tta aaa acc caa ctg cct tca ggt tca gaa ctc tct ctg         96
Ala Leu Leu Leu Lys Thr Gln Leu Pro Ser Gly Ser Glu Leu Ser Leu           
            20                  25                  30                    

tat gat atc gct cca gtg act ccc ggt gtg gct gtc gat ctg agc cat        144
Tyr Asp Ile Ala Pro Val Thr Pro Gly Val Ala Val Asp Leu Ser His           
        35                  40                  45                        

atc cct act gct gtg aaa atc aaa ggt ttt tct ggt gaa gat gcg act        192
Ile Pro Thr Ala Val Lys Ile Lys Gly Phe Ser Gly Glu Asp Ala Thr           
    50                  55                  60                            

ccg gcg ctg gaa ggc gca gat gtc gtt ctt atc tct gca ggc gta gcg        240
Pro Ala Leu Glu Gly Ala Asp Val Val Leu Ile Ser Ala Gly Val Ala           
65                  70                  75                  80            

cgt aaa ccg ggt atg gat cgt tcc gac ctg ttt aac gtt aac gcc ggc        288
Arg Lys Pro Gly Met Asp Arg Ser Asp Leu Phe Asn Val Asn Ala Gly           
                85                  90                  95                

atc gtg aaa aac ctg gta cag caa gtt gcg aaa acc tgc ccg aaa gcg        336
Ile Val Lys Asn Leu Val Gln Gln Val Ala Lys Thr Cys Pro Lys Ala           
            100                 105                 110                   

tgc att ggt att atc act aac ccg gtt aac acc aca gtt gca att gct        384
Cys Ile Gly Ile Ile Thr Asn Pro Val Asn Thr Thr Val Ala Ile Ala           
        115                 120                 125                       

gct gaa gtg ctg aaa aaa gcc ggt gtt tat gac aaa aac aaa ctg ttc        432
Ala Glu Val Leu Lys Lys Ala Gly Val Tyr Asp Lys Asn Lys Leu Phe           
    130                 135                 140                           

ggc gtt acc acg ctg gat atc att cgt tcc aac acc ttt gtt gcg gaa        480
Gly Val Thr Thr Leu Asp Ile Ile Arg Ser Asn Thr Phe Val Ala Glu           
145                 150                 155                 160           

ctg aaa ggc aaa cag cca ggc gaa gtt gaa gtg ccg gtt att ggc ggt        528
Leu Lys Gly Lys Gln Pro Gly Glu Val Glu Val Pro Val Ile Gly Gly           
                165                 170                 175               

cac tct ggt gtt acc att ctg ccg ctg ctg tca cag gtt cct ggc gtt        576
His Ser Gly Val Thr Ile Leu Pro Leu Leu Ser Gln Val Pro Gly Val           
            180                 185                 190                   

agt ttt acc gag cag gaa gtg gct gat ctg acc aaa cgc atc cag aac        624
Ser Phe Thr Glu Gln Glu Val Ala Asp Leu Thr Lys Arg Ile Gln Asn           
        195                 200                 205                       

gcg ggt act gaa gtg gtt gaa gcg aag gcc ggt ggc ggg tct gca acc        672
Ala Gly Thr Glu Val Val Glu Ala Lys Ala Gly Gly Gly Ser Ala Thr           
    210                 215                 220                           

ctg tct atg ggc cag gca gct gca cgt ttt ggt ctg tct ctg gtt cgt        720
Leu Ser Met Gly Gln Ala Ala Ala Arg Phe Gly Leu Ser Leu Val Arg           
225                 230                 235                 240           

gca ctg cag ggc gaa caa ggc gtt gtc gaa tgt gcc tac gtt gaa ggc        768
Ala Leu Gln Gly Glu Gln Gly Val Val Glu Cys Ala Tyr Val Glu Gly           
                245                 250                 255               

gac ggt cag tac gcc cgt ttc ttc tct caa ccg ctg ctg ctg ggt aaa        816
Asp Gly Gln Tyr Ala Arg Phe Phe Ser Gln Pro Leu Leu Leu Gly Lys           
            260                 265                 270                   

aac ggc gtg gaa gag cgt aaa tct atc ggt acc ctg agc gca ttt gaa        864
Asn Gly Val Glu Glu Arg Lys Ser Ile Gly Thr Leu Ser Ala Phe Glu           
        275                 280                 285                       

cag aac gcg ctg gaa ggt atg ctg gat acg ctg aag aaa gat atc gcc        912
Gln Asn Ala Leu Glu Gly Met Leu Asp Thr Leu Lys Lys Asp Ile Ala           
    290                 295                 300                           

ctg ggc gaa gag ttc gtt aat aag taa                                    939
Leu Gly Glu Glu Phe Val Asn Lys                                           
305                 310                                                   


<210>  28
<211>  312
<212>  PRT
<213>  Escherichia coli

<400>  28

Met Lys Val Ala Val Leu Gly Ala Ala Gly Gly Ile Gly Gln Ala Leu 
1               5                   10                  15      


Ala Leu Leu Leu Lys Thr Gln Leu Pro Ser Gly Ser Glu Leu Ser Leu 
            20                  25                  30          


Tyr Asp Ile Ala Pro Val Thr Pro Gly Val Ala Val Asp Leu Ser His 
        35                  40                  45              


Ile Pro Thr Ala Val Lys Ile Lys Gly Phe Ser Gly Glu Asp Ala Thr 
    50                  55                  60                  


Pro Ala Leu Glu Gly Ala Asp Val Val Leu Ile Ser Ala Gly Val Ala 
65                  70                  75                  80  


Arg Lys Pro Gly Met Asp Arg Ser Asp Leu Phe Asn Val Asn Ala Gly 
                85                  90                  95      


Ile Val Lys Asn Leu Val Gln Gln Val Ala Lys Thr Cys Pro Lys Ala 
            100                 105                 110         


Cys Ile Gly Ile Ile Thr Asn Pro Val Asn Thr Thr Val Ala Ile Ala 
        115                 120                 125             


Ala Glu Val Leu Lys Lys Ala Gly Val Tyr Asp Lys Asn Lys Leu Phe 
    130                 135                 140                 


Gly Val Thr Thr Leu Asp Ile Ile Arg Ser Asn Thr Phe Val Ala Glu 
145                 150                 155                 160 


Leu Lys Gly Lys Gln Pro Gly Glu Val Glu Val Pro Val Ile Gly Gly 
                165                 170                 175     


His Ser Gly Val Thr Ile Leu Pro Leu Leu Ser Gln Val Pro Gly Val 
            180                 185                 190         


Ser Phe Thr Glu Gln Glu Val Ala Asp Leu Thr Lys Arg Ile Gln Asn 
        195                 200                 205             


Ala Gly Thr Glu Val Val Glu Ala Lys Ala Gly Gly Gly Ser Ala Thr 
    210                 215                 220                 


Leu Ser Met Gly Gln Ala Ala Ala Arg Phe Gly Leu Ser Leu Val Arg 
225                 230                 235                 240 


Ala Leu Gln Gly Glu Gln Gly Val Val Glu Cys Ala Tyr Val Glu Gly 
                245                 250                 255     


Asp Gly Gln Tyr Ala Arg Phe Phe Ser Gln Pro Leu Leu Leu Gly Lys 
            260                 265                 270         


Asn Gly Val Glu Glu Arg Lys Ser Ile Gly Thr Leu Ser Ala Phe Glu 
        275                 280                 285             


Gln Asn Ala Leu Glu Gly Met Leu Asp Thr Leu Lys Lys Asp Ile Ala 
    290                 295                 300                 


Leu Gly Glu Glu Phe Val Asn Lys 
305                 310         


<210>  29
<211>  1413
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(1413)

<400>  29
atg tct ctc tct ccc gtt gtt gtt att gga acc ggt ttg gcc ggg ctg         48
Met Ser Leu Ser Pro Val Val Val Ile Gly Thr Gly Leu Ala Gly Leu           
1               5                   10                  15                

gct gct gcc aac gaa ttg gtt aac aag tat aac atc cct gta acc atc         96
Ala Ala Ala Asn Glu Leu Val Asn Lys Tyr Asn Ile Pro Val Thr Ile           
            20                  25                  30                    

ctc gaa aag gct tcc tcg atc ggt ggg aac tct atc aag gcc tcc agt        144
Leu Glu Lys Ala Ser Ser Ile Gly Gly Asn Ser Ile Lys Ala Ser Ser           
        35                  40                  45                        

ggt att aac ggt gct tgc acc gag act caa cgt cac ttc cac atc gag        192
Gly Ile Asn Gly Ala Cys Thr Glu Thr Gln Arg His Phe His Ile Glu           
    50                  55                  60                            

gac tcc cca cgc tta ttt gaa gat gac acc atc aag tct gct aaa ggt        240
Asp Ser Pro Arg Leu Phe Glu Asp Asp Thr Ile Lys Ser Ala Lys Gly           
65                  70                  75                  80            

aaa ggt gtc caa gag tta atg gct aag ttg gcc aat gat tct ccc ctg        288
Lys Gly Val Gln Glu Leu Met Ala Lys Leu Ala Asn Asp Ser Pro Leu           
                85                  90                  95                

gct att gaa tgg ttg aaa aac gaa ttt gat ttg aaa ttg gac cta ttg        336
Ala Ile Glu Trp Leu Lys Asn Glu Phe Asp Leu Lys Leu Asp Leu Leu           
            100                 105                 110                   

gct caa ttg ggt ggc cac tct gtg gca aga act cac aga tcg tct ggg        384
Ala Gln Leu Gly Gly His Ser Val Ala Arg Thr His Arg Ser Ser Gly           
        115                 120                 125                       

aag ttg cct cca ggt ttc gaa att gtt tct gcc tta tct aac aat ttg        432
Lys Leu Pro Pro Gly Phe Glu Ile Val Ser Ala Leu Ser Asn Asn Leu           
    130                 135                 140                           

aag aaa tta gct gag act aaa cca gag tta gtt aag att aac tta gac        480
Lys Lys Leu Ala Glu Thr Lys Pro Glu Leu Val Lys Ile Asn Leu Asp           
145                 150                 155                 160           

agt aaa gtc gta gac atc cat gaa aag gat ggc tcc att tct gct gta        528
Ser Lys Val Val Asp Ile His Glu Lys Asp Gly Ser Ile Ser Ala Val           
                165                 170                 175               

gtg tac gag gat aag aat ggc gaa aag cac atg gtg agt gct aac gat        576
Val Tyr Glu Asp Lys Asn Gly Glu Lys His Met Val Ser Ala Asn Asp           
            180                 185                 190                   

gtc gtt ttt tgt tct gga ggg ttt ggc ttt tct aag gaa atg ctt aaa        624
Val Val Phe Cys Ser Gly Gly Phe Gly Phe Ser Lys Glu Met Leu Lys           
        195                 200                 205                       

gaa tat gca ccc gaa ctg gtg aac ttg cca acg aca aac ggg caa caa        672
Glu Tyr Ala Pro Glu Leu Val Asn Leu Pro Thr Thr Asn Gly Gln Gln           
    210                 215                 220                           

aca act ggt gat ggt caa agg ctt ctg cag aag tta ggc gct gat ctg        720
Thr Thr Gly Asp Gly Gln Arg Leu Leu Gln Lys Leu Gly Ala Asp Leu           
225                 230                 235                 240           

att gac atg gac caa att caa gtt cat cca act ggg ttc att gat cca        768
Ile Asp Met Asp Gln Ile Gln Val His Pro Thr Gly Phe Ile Asp Pro           
                245                 250                 255               

aat gac cgt agc tca agc tgg aaa ttc ttg gct gcc gaa tcc tta aga        816
Asn Asp Arg Ser Ser Ser Trp Lys Phe Leu Ala Ala Glu Ser Leu Arg           
            260                 265                 270                   

ggt ctt ggt ggt atc cta tta aac cct att acc ggt aga aga ttt gtc        864
Gly Leu Gly Gly Ile Leu Leu Asn Pro Ile Thr Gly Arg Arg Phe Val           
        275                 280                 285                       

aac gaa ttg acc aca aga gat gta gtc act gca gct att caa aag gtt        912
Asn Glu Leu Thr Thr Arg Asp Val Val Thr Ala Ala Ile Gln Lys Val           
    290                 295                 300                           

tgt cct caa gag gat aac aga gca cta ttg gtt atg ggc gaa aaa atg        960
Cys Pro Gln Glu Asp Asn Arg Ala Leu Leu Val Met Gly Glu Lys Met           
305                 310                 315                 320           

tac aca gat ttg aag aat aat tta gat ttt tac atg ttc aag aaa ctt       1008
Tyr Thr Asp Leu Lys Asn Asn Leu Asp Phe Tyr Met Phe Lys Lys Leu           
                325                 330                 335               

gta cag aaa ttg aca tta tct caa gtt gtg tct gaa tat aat tta cca       1056
Val Gln Lys Leu Thr Leu Ser Gln Val Val Ser Glu Tyr Asn Leu Pro           
            340                 345                 350                   

atc act gtc acc caa tta tgc gag gaa ttg caa aca tac tct tcg ttc       1104
Ile Thr Val Thr Gln Leu Cys Glu Glu Leu Gln Thr Tyr Ser Ser Phe           
        355                 360                 365                       

act acc aag gct gat ccg ttg gga cgt acc gtt att ctc aac gaa ttt       1152
Thr Thr Lys Ala Asp Pro Leu Gly Arg Thr Val Ile Leu Asn Glu Phe           
    370                 375                 380                           

ggc tct gac gtt act cca gaa acc gtg gtt ttt att ggt gaa gta aca       1200
Gly Ser Asp Val Thr Pro Glu Thr Val Val Phe Ile Gly Glu Val Thr           
385                 390                 395                 400           

ccg gtt gtc cat ttc acc atg ggt ggt gct aga atc aat gtc aag gct       1248
Pro Val Val His Phe Thr Met Gly Gly Ala Arg Ile Asn Val Lys Ala           
                405                 410                 415               

caa gtc att ggc aag aac gac gaa agg cta cta aaa ggc ctg tac gcg       1296
Gln Val Ile Gly Lys Asn Asp Glu Arg Leu Leu Lys Gly Leu Tyr Ala           
            420                 425                 430                   

gcc ggt gaa gtt tct ggc ggt gtt cat ggc gcc aat agg ttg ggt ggt       1344
Ala Gly Glu Val Ser Gly Gly Val His Gly Ala Asn Arg Leu Gly Gly           
        435                 440                 445                       

tca agt ttg tta gaa tgc gtt gtc ttt ggg aga act gca gct gaa tct       1392
Ser Ser Leu Leu Glu Cys Val Val Phe Gly Arg Thr Ala Ala Glu Ser           
    450                 455                 460                           

att gcc aat gac cgc aag taa                                           1413
Ile Ala Asn Asp Arg Lys                                                   
465                 470                                                   


<210>  30
<211>  470
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  30

Met Ser Leu Ser Pro Val Val Val Ile Gly Thr Gly Leu Ala Gly Leu 
1               5                   10                  15      


Ala Ala Ala Asn Glu Leu Val Asn Lys Tyr Asn Ile Pro Val Thr Ile 
            20                  25                  30          


Leu Glu Lys Ala Ser Ser Ile Gly Gly Asn Ser Ile Lys Ala Ser Ser 
        35                  40                  45              


Gly Ile Asn Gly Ala Cys Thr Glu Thr Gln Arg His Phe His Ile Glu 
    50                  55                  60                  


Asp Ser Pro Arg Leu Phe Glu Asp Asp Thr Ile Lys Ser Ala Lys Gly 
65                  70                  75                  80  


Lys Gly Val Gln Glu Leu Met Ala Lys Leu Ala Asn Asp Ser Pro Leu 
                85                  90                  95      


Ala Ile Glu Trp Leu Lys Asn Glu Phe Asp Leu Lys Leu Asp Leu Leu 
            100                 105                 110         


Ala Gln Leu Gly Gly His Ser Val Ala Arg Thr His Arg Ser Ser Gly 
        115                 120                 125             


Lys Leu Pro Pro Gly Phe Glu Ile Val Ser Ala Leu Ser Asn Asn Leu 
    130                 135                 140                 


Lys Lys Leu Ala Glu Thr Lys Pro Glu Leu Val Lys Ile Asn Leu Asp 
145                 150                 155                 160 


Ser Lys Val Val Asp Ile His Glu Lys Asp Gly Ser Ile Ser Ala Val 
                165                 170                 175     


Val Tyr Glu Asp Lys Asn Gly Glu Lys His Met Val Ser Ala Asn Asp 
            180                 185                 190         


Val Val Phe Cys Ser Gly Gly Phe Gly Phe Ser Lys Glu Met Leu Lys 
        195                 200                 205             


Glu Tyr Ala Pro Glu Leu Val Asn Leu Pro Thr Thr Asn Gly Gln Gln 
    210                 215                 220                 


Thr Thr Gly Asp Gly Gln Arg Leu Leu Gln Lys Leu Gly Ala Asp Leu 
225                 230                 235                 240 


Ile Asp Met Asp Gln Ile Gln Val His Pro Thr Gly Phe Ile Asp Pro 
                245                 250                 255     


Asn Asp Arg Ser Ser Ser Trp Lys Phe Leu Ala Ala Glu Ser Leu Arg 
            260                 265                 270         


Gly Leu Gly Gly Ile Leu Leu Asn Pro Ile Thr Gly Arg Arg Phe Val 
        275                 280                 285             


Asn Glu Leu Thr Thr Arg Asp Val Val Thr Ala Ala Ile Gln Lys Val 
    290                 295                 300                 


Cys Pro Gln Glu Asp Asn Arg Ala Leu Leu Val Met Gly Glu Lys Met 
305                 310                 315                 320 


Tyr Thr Asp Leu Lys Asn Asn Leu Asp Phe Tyr Met Phe Lys Lys Leu 
                325                 330                 335     


Val Gln Lys Leu Thr Leu Ser Gln Val Val Ser Glu Tyr Asn Leu Pro 
            340                 345                 350         


Ile Thr Val Thr Gln Leu Cys Glu Glu Leu Gln Thr Tyr Ser Ser Phe 
        355                 360                 365             


Thr Thr Lys Ala Asp Pro Leu Gly Arg Thr Val Ile Leu Asn Glu Phe 
    370                 375                 380                 


Gly Ser Asp Val Thr Pro Glu Thr Val Val Phe Ile Gly Glu Val Thr 
385                 390                 395                 400 


Pro Val Val His Phe Thr Met Gly Gly Ala Arg Ile Asn Val Lys Ala 
                405                 410                 415     


Gln Val Ile Gly Lys Asn Asp Glu Arg Leu Leu Lys Gly Leu Tyr Ala 
            420                 425                 430         


Ala Gly Glu Val Ser Gly Gly Val His Gly Ala Asn Arg Leu Gly Gly 
        435                 440                 445             


Ser Ser Leu Leu Glu Cys Val Val Phe Gly Arg Thr Ala Ala Glu Ser 
    450                 455                 460                 


Ile Ala Asn Asp Arg Lys 
465                 470 


<210>  31
<211>  1413
<212>  DNA
<213>  Saccharomyces mikatae


<220>
<221>  CDS
<222>  (1)..(1413)

<400>  31
atg tca tct tct cca gtt gtc gtt att ggt aca ggc ttg gca ggt ttg         48
Met Ser Ser Ser Pro Val Val Val Ile Gly Thr Gly Leu Ala Gly Leu           
1               5                   10                  15                

gca act gct aat gag tta gtc aat aag tac aac att cct gtt acc att         96
Ala Thr Ala Asn Glu Leu Val Asn Lys Tyr Asn Ile Pro Val Thr Ile           
            20                  25                  30                    

ttg gaa aag gca tcc tct atc ggt ggc aat tcc att aag gca tct tct        144
Leu Glu Lys Ala Ser Ser Ile Gly Gly Asn Ser Ile Lys Ala Ser Ser           
        35                  40                  45                        

ggt atc aat ggt gca tgt aca gaa acc caa cgt cat ttt cac att gaa        192
Gly Ile Asn Gly Ala Cys Thr Glu Thr Gln Arg His Phe His Ile Glu           
    50                  55                  60                            

gat act cct aga ctt ttt gaa gat gat act gtt caa tcc gcc aag ggc        240
Asp Thr Pro Arg Leu Phe Glu Asp Asp Thr Val Gln Ser Ala Lys Gly           
65                  70                  75                  80            

aaa ggt gtt caa gag tta atg ggt aaa ctt gct aat gat tct cca ctt        288
Lys Gly Val Gln Glu Leu Met Gly Lys Leu Ala Asn Asp Ser Pro Leu           
                85                  90                  95                

gct att gaa tgg tta aag act gaa ttc gac tta aag tta gac ctt ttg        336
Ala Ile Glu Trp Leu Lys Thr Glu Phe Asp Leu Lys Leu Asp Leu Leu           
            100                 105                 110                   

gct cag tta ggt ggt cac tct gtt gct aga act cat aga tct tcc ggt        384
Ala Gln Leu Gly Gly His Ser Val Ala Arg Thr His Arg Ser Ser Gly           
        115                 120                 125                       

aaa ctt cca cca ggt ttc gaa atc gtt tcc gcc tta tcc aat aac ttg        432
Lys Leu Pro Pro Gly Phe Glu Ile Val Ser Ala Leu Ser Asn Asn Leu           
    130                 135                 140                           

aaa aag ttg gca gaa acc aag cca gag tta gtt aag att aac tta gac        480
Lys Lys Leu Ala Glu Thr Lys Pro Glu Leu Val Lys Ile Asn Leu Asp           
145                 150                 155                 160           

tca aag gtc gtt gac atc cac aaa aag gac ggc tct att tcc gca att        528
Ser Lys Val Val Asp Ile His Lys Lys Asp Gly Ser Ile Ser Ala Ile           
                165                 170                 175               

gtc tat gat gac aaa aac ggt gaa aga cat acc tta tcc act tca aat        576
Val Tyr Asp Asp Lys Asn Gly Glu Arg His Thr Leu Ser Thr Ser Asn           
            180                 185                 190                   

gtt gtt ttc tgc tct ggt ggt ttc ggt ttt tct aag gaa atg tta aac        624
Val Val Phe Cys Ser Gly Gly Phe Gly Phe Ser Lys Glu Met Leu Asn           
        195                 200                 205                       

gag tat gct cca caa ttg gtc aac ttg cca acc act aac ggt cag caa        672
Glu Tyr Ala Pro Gln Leu Val Asn Leu Pro Thr Thr Asn Gly Gln Gln           
    210                 215                 220                           

aca aca ggt gac ggc caa aga ttg tta caa aag ctt ggt gca gat ttg        720
Thr Thr Gly Asp Gly Gln Arg Leu Leu Gln Lys Leu Gly Ala Asp Leu           
225                 230                 235                 240           

att gat atg gat caa att caa gtc cat cct act ggt ttc atc gac cca        768
Ile Asp Met Asp Gln Ile Gln Val His Pro Thr Gly Phe Ile Asp Pro           
                245                 250                 255               

aac gat aga aac tcc tct tgg aag ttt ttg gct gct gaa tct tta aga        816
Asn Asp Arg Asn Ser Ser Trp Lys Phe Leu Ala Ala Glu Ser Leu Arg           
            260                 265                 270                   

ggt ttg ggt ggt atc tta ttg aat cca att act ggt cgt aga ttt gtc        864
Gly Leu Gly Gly Ile Leu Leu Asn Pro Ile Thr Gly Arg Arg Phe Val           
        275                 280                 285                       

aac gaa ttg acc act aga gat gtc gtt act gaa gca atc cag aag cac        912
Asn Glu Leu Thr Thr Arg Asp Val Val Thr Glu Ala Ile Gln Lys His           
    290                 295                 300                           

tgt cca caa gat gat aac aga gct ttg tta gtt atg tcc gaa aag atg        960
Cys Pro Gln Asp Asp Asn Arg Ala Leu Leu Val Met Ser Glu Lys Met           
305                 310                 315                 320           

tat aca gat ttg aaa aac aat ttg gac ttc tac atg ttc aaa aag tta       1008
Tyr Thr Asp Leu Lys Asn Asn Leu Asp Phe Tyr Met Phe Lys Lys Leu           
                325                 330                 335               

gtt caa aag tta tct ttg tcc caa gtt gtt tcc gag tat aag tta cca       1056
Val Gln Lys Leu Ser Leu Ser Gln Val Val Ser Glu Tyr Lys Leu Pro           
            340                 345                 350                   

att act gtt tcc caa ttg tgt cag gaa tta caa acc tac tca tct ttt       1104
Ile Thr Val Ser Gln Leu Cys Gln Glu Leu Gln Thr Tyr Ser Ser Phe           
        355                 360                 365                       

act tca aaa gcc gat cct ctt ggt aga acc gtt gtc tta aac gaa ttc       1152
Thr Ser Lys Ala Asp Pro Leu Gly Arg Thr Val Val Leu Asn Glu Phe           
    370                 375                 380                           

ggt gct gac atc acc cca gaa aca atg gtt ttc atc ggc gaa gtt acc       1200
Gly Ala Asp Ile Thr Pro Glu Thr Met Val Phe Ile Gly Glu Val Thr           
385                 390                 395                 400           

cca gtc gtt cac ttt acc atg ggt ggt gct aga atc aat gtt aag gct       1248
Pro Val Val His Phe Thr Met Gly Gly Ala Arg Ile Asn Val Lys Ala           
                405                 410                 415               

caa gtt atc ggc aaa aac gat gag cct ttg tta aac ggt ttg tac gca       1296
Gln Val Ile Gly Lys Asn Asp Glu Pro Leu Leu Asn Gly Leu Tyr Ala           
            420                 425                 430                   

gca ggt gaa gtt tct ggt ggt gtc cat ggt gcc aat aga tta ggt ggt       1344
Ala Gly Glu Val Ser Gly Gly Val His Gly Ala Asn Arg Leu Gly Gly           
        435                 440                 445                       

tca tct ttg ctt gaa tgt gtc gtt ttt ggt aga act gca gca gaa tca       1392
Ser Ser Leu Leu Glu Cys Val Val Phe Gly Arg Thr Ala Ala Glu Ser           
    450                 455                 460                           

att gcc aat aac cac aag taa                                           1413
Ile Ala Asn Asn His Lys                                                   
465                 470                                                   


<210>  32
<211>  470
<212>  PRT
<213>  Saccharomyces mikatae

<400>  32

Met Ser Ser Ser Pro Val Val Val Ile Gly Thr Gly Leu Ala Gly Leu 
1               5                   10                  15      


Ala Thr Ala Asn Glu Leu Val Asn Lys Tyr Asn Ile Pro Val Thr Ile 
            20                  25                  30          


Leu Glu Lys Ala Ser Ser Ile Gly Gly Asn Ser Ile Lys Ala Ser Ser 
        35                  40                  45              


Gly Ile Asn Gly Ala Cys Thr Glu Thr Gln Arg His Phe His Ile Glu 
    50                  55                  60                  


Asp Thr Pro Arg Leu Phe Glu Asp Asp Thr Val Gln Ser Ala Lys Gly 
65                  70                  75                  80  


Lys Gly Val Gln Glu Leu Met Gly Lys Leu Ala Asn Asp Ser Pro Leu 
                85                  90                  95      


Ala Ile Glu Trp Leu Lys Thr Glu Phe Asp Leu Lys Leu Asp Leu Leu 
            100                 105                 110         


Ala Gln Leu Gly Gly His Ser Val Ala Arg Thr His Arg Ser Ser Gly 
        115                 120                 125             


Lys Leu Pro Pro Gly Phe Glu Ile Val Ser Ala Leu Ser Asn Asn Leu 
    130                 135                 140                 


Lys Lys Leu Ala Glu Thr Lys Pro Glu Leu Val Lys Ile Asn Leu Asp 
145                 150                 155                 160 


Ser Lys Val Val Asp Ile His Lys Lys Asp Gly Ser Ile Ser Ala Ile 
                165                 170                 175     


Val Tyr Asp Asp Lys Asn Gly Glu Arg His Thr Leu Ser Thr Ser Asn 
            180                 185                 190         


Val Val Phe Cys Ser Gly Gly Phe Gly Phe Ser Lys Glu Met Leu Asn 
        195                 200                 205             


Glu Tyr Ala Pro Gln Leu Val Asn Leu Pro Thr Thr Asn Gly Gln Gln 
    210                 215                 220                 


Thr Thr Gly Asp Gly Gln Arg Leu Leu Gln Lys Leu Gly Ala Asp Leu 
225                 230                 235                 240 


Ile Asp Met Asp Gln Ile Gln Val His Pro Thr Gly Phe Ile Asp Pro 
                245                 250                 255     


Asn Asp Arg Asn Ser Ser Trp Lys Phe Leu Ala Ala Glu Ser Leu Arg 
            260                 265                 270         


Gly Leu Gly Gly Ile Leu Leu Asn Pro Ile Thr Gly Arg Arg Phe Val 
        275                 280                 285             


Asn Glu Leu Thr Thr Arg Asp Val Val Thr Glu Ala Ile Gln Lys His 
    290                 295                 300                 


Cys Pro Gln Asp Asp Asn Arg Ala Leu Leu Val Met Ser Glu Lys Met 
305                 310                 315                 320 


Tyr Thr Asp Leu Lys Asn Asn Leu Asp Phe Tyr Met Phe Lys Lys Leu 
                325                 330                 335     


Val Gln Lys Leu Ser Leu Ser Gln Val Val Ser Glu Tyr Lys Leu Pro 
            340                 345                 350         


Ile Thr Val Ser Gln Leu Cys Gln Glu Leu Gln Thr Tyr Ser Ser Phe 
        355                 360                 365             


Thr Ser Lys Ala Asp Pro Leu Gly Arg Thr Val Val Leu Asn Glu Phe 
    370                 375                 380                 


Gly Ala Asp Ile Thr Pro Glu Thr Met Val Phe Ile Gly Glu Val Thr 
385                 390                 395                 400 


Pro Val Val His Phe Thr Met Gly Gly Ala Arg Ile Asn Val Lys Ala 
                405                 410                 415     


Gln Val Ile Gly Lys Asn Asp Glu Pro Leu Leu Asn Gly Leu Tyr Ala 
            420                 425                 430         


Ala Gly Glu Val Ser Gly Gly Val His Gly Ala Asn Arg Leu Gly Gly 
        435                 440                 445             


Ser Ser Leu Leu Glu Cys Val Val Phe Gly Arg Thr Ala Ala Glu Ser 
    450                 455                 460                 


Ile Ala Asn Asn His Lys 
465                 470 


<210>  33
<211>  1413
<212>  DNA
<213>  Kluyveromyces polyspora


<220>
<221>  CDS
<222>  (1)..(1413)

<400>  33
atg tca acc aaa aag cca gtc gtc atc att ggt act ggt tta gcc ggt         48
Met Ser Thr Lys Lys Pro Val Val Ile Ile Gly Thr Gly Leu Ala Gly           
1               5                   10                  15                

ttg tct gct ggt aat caa ttg gtc aat atg cat aaa gtt cct atc att         96
Leu Ser Ala Gly Asn Gln Leu Val Asn Met His Lys Val Pro Ile Ile           
            20                  25                  30                    

atg ttg gac aag gca tcc tcc att ggt ggt aat tct aca aag gct tcc        144
Met Leu Asp Lys Ala Ser Ser Ile Gly Gly Asn Ser Thr Lys Ala Ser           
        35                  40                  45                        

tct ggt atc aac ggt gct tct act att act caa cag caa ctt aat gtt        192
Ser Gly Ile Asn Gly Ala Ser Thr Ile Thr Gln Gln Gln Leu Asn Val           
    50                  55                  60                            

aaa gac tct cct gac tta ttc ctt caa gat act gtt aag tct gct aag        240
Lys Asp Ser Pro Asp Leu Phe Leu Gln Asp Thr Val Lys Ser Ala Lys           
65                  70                  75                  80            

ggt aga ggt att gag tcc ctt atg aaa aag tta tca caa gac tcc aac        288
Gly Arg Gly Ile Glu Ser Leu Met Lys Lys Leu Ser Gln Asp Ser Asn           
                85                  90                  95                

tct gct atc cat tgg ttg caa cag gat ttt gat ttg aag ttg gat ttg        336
Ser Ala Ile His Trp Leu Gln Gln Asp Phe Asp Leu Lys Leu Asp Leu           
            100                 105                 110                   

tta gct caa ttg ggt ggt cat tcc gtt cct aga aca cac cgt tcc tca        384
Leu Ala Gln Leu Gly Gly His Ser Val Pro Arg Thr His Arg Ser Ser           
        115                 120                 125                       

ggc aag tta cct cca ggc ttc gaa att gtc caa gct tta tct aac aag        432
Gly Lys Leu Pro Pro Gly Phe Glu Ile Val Gln Ala Leu Ser Asn Lys           
    130                 135                 140                           

tta aag gct att tct gag tcc gat cca gaa ttc gtt aga atc tta ctt        480
Leu Lys Ala Ile Ser Glu Ser Asp Pro Glu Phe Val Arg Ile Leu Leu           
145                 150                 155                 160           

aac tcc aag gtt gtt gat gtt tcc gtt aac aat gag ggc aag gtc gaa        528
Asn Ser Lys Val Val Asp Val Ser Val Asn Asn Glu Gly Lys Val Glu           
                165                 170                 175               

tct att gac tat gtt gat gca gaa ggt aaa cat cac aaa atc gct act        576
Ser Ile Asp Tyr Val Asp Ala Glu Gly Lys His His Lys Ile Ala Thr           
            180                 185                 190                   

gat aac gtt gtc ttt tgt tcc ggt ggt ttc ggt cac tca gca gaa atg        624
Asp Asn Val Val Phe Cys Ser Gly Gly Phe Gly His Ser Ala Glu Met           
        195                 200                 205                       

ttg aac aag tat gca cca gaa tta gct aac ttg cca act act aac ggt        672
Leu Asn Lys Tyr Ala Pro Glu Leu Ala Asn Leu Pro Thr Thr Asn Gly           
    210                 215                 220                           

caa caa acc act ggc gat ggt cag aga atc ttg gag aaa ttg ggt gca        720
Gln Gln Thr Thr Gly Asp Gly Gln Arg Ile Leu Glu Lys Leu Gly Ala           
225                 230                 235                 240           

gac ttg att gat atg tcc caa att caa gtt cac cca aca ggt ttc atc        768
Asp Leu Ile Asp Met Ser Gln Ile Gln Val His Pro Thr Gly Phe Ile           
                245                 250                 255               

gat cca gca aac aga gat tct aag tgg aag ttt ttg gct gcc gaa gca        816
Asp Pro Ala Asn Arg Asp Ser Lys Trp Lys Phe Leu Ala Ala Glu Ala           
            260                 265                 270                   

tta aga ggt tta ggt ggt atc tta ctt aat cca tct acc ggc aag aga        864
Leu Arg Gly Leu Gly Gly Ile Leu Leu Asn Pro Ser Thr Gly Lys Arg           
        275                 280                 285                       

ttc gtt aat gag tta acc acc aga gat ttg gtc aca gaa gct atc caa        912
Phe Val Asn Glu Leu Thr Thr Arg Asp Leu Val Thr Glu Ala Ile Gln           
    290                 295                 300                           

tca caa tgt cca aga gat gac aat aag gca ttc ctt gtt atg tct gaa        960
Ser Gln Cys Pro Arg Asp Asp Asn Lys Ala Phe Leu Val Met Ser Glu           
305                 310                 315                 320           

aag gtc tat gag aat tac aaa aac aac atg gac ttt tac tta ttc aaa       1008
Lys Val Tyr Glu Asn Tyr Lys Asn Asn Met Asp Phe Tyr Leu Phe Lys           
                325                 330                 335               

aag tta gtt tcc aag atg acc att aag gaa ttt gtc gaa act tac aag       1056
Lys Leu Val Ser Lys Met Thr Ile Lys Glu Phe Val Glu Thr Tyr Lys           
            340                 345                 350                   

ttg cca att tct gcc gac gcc gtt acc caa gac tta atc gac tat tca       1104
Leu Pro Ile Ser Ala Asp Ala Val Thr Gln Asp Leu Ile Asp Tyr Ser           
        355                 360                 365                       

gtt gat aag acc gat aag ttt ggt aga cca ttg gtt atc aac gtt ttt       1152
Val Asp Lys Thr Asp Lys Phe Gly Arg Pro Leu Val Ile Asn Val Phe           
    370                 375                 380                           

gat gaa aag ttg acc gaa gat tcc gaa atc tat gtt ggt gaa gtt aca       1200
Asp Glu Lys Leu Thr Glu Asp Ser Glu Ile Tyr Val Gly Glu Val Thr           
385                 390                 395                 400           

cca gtt gtc cat ttc act atg ggt ggt gca aag atc aat act gaa tct       1248
Pro Val Val His Phe Thr Met Gly Gly Ala Lys Ile Asn Thr Glu Ser           
                405                 410                 415               

caa gtt atc aac aaa aac ggt caa gtt ttg gca aag ggt atc tac gca       1296
Gln Val Ile Asn Lys Asn Gly Gln Val Leu Ala Lys Gly Ile Tyr Ala           
            420                 425                 430                   

gca ggt gaa gtc tcc ggt ggt gtt cac ggt tct aat aga tta ggt ggt       1344
Ala Gly Glu Val Ser Gly Gly Val His Gly Ser Asn Arg Leu Gly Gly           
        435                 440                 445                       

tca tct ttg tta gaa tgc gtc gtt tac ggt aga tct gct gca gat aac       1392
Ser Ser Leu Leu Glu Cys Val Val Tyr Gly Arg Ser Ala Ala Asp Asn           
    450                 455                 460                           

att gcc aaa aac att gaa taa                                           1413
Ile Ala Lys Asn Ile Glu                                                   
465                 470                                                   


<210>  34
<211>  470
<212>  PRT
<213>  Kluyveromyces polyspora

<400>  34

Met Ser Thr Lys Lys Pro Val Val Ile Ile Gly Thr Gly Leu Ala Gly 
1               5                   10                  15      


Leu Ser Ala Gly Asn Gln Leu Val Asn Met His Lys Val Pro Ile Ile 
            20                  25                  30          


Met Leu Asp Lys Ala Ser Ser Ile Gly Gly Asn Ser Thr Lys Ala Ser 
        35                  40                  45              


Ser Gly Ile Asn Gly Ala Ser Thr Ile Thr Gln Gln Gln Leu Asn Val 
    50                  55                  60                  


Lys Asp Ser Pro Asp Leu Phe Leu Gln Asp Thr Val Lys Ser Ala Lys 
65                  70                  75                  80  


Gly Arg Gly Ile Glu Ser Leu Met Lys Lys Leu Ser Gln Asp Ser Asn 
                85                  90                  95      


Ser Ala Ile His Trp Leu Gln Gln Asp Phe Asp Leu Lys Leu Asp Leu 
            100                 105                 110         


Leu Ala Gln Leu Gly Gly His Ser Val Pro Arg Thr His Arg Ser Ser 
        115                 120                 125             


Gly Lys Leu Pro Pro Gly Phe Glu Ile Val Gln Ala Leu Ser Asn Lys 
    130                 135                 140                 


Leu Lys Ala Ile Ser Glu Ser Asp Pro Glu Phe Val Arg Ile Leu Leu 
145                 150                 155                 160 


Asn Ser Lys Val Val Asp Val Ser Val Asn Asn Glu Gly Lys Val Glu 
                165                 170                 175     


Ser Ile Asp Tyr Val Asp Ala Glu Gly Lys His His Lys Ile Ala Thr 
            180                 185                 190         


Asp Asn Val Val Phe Cys Ser Gly Gly Phe Gly His Ser Ala Glu Met 
        195                 200                 205             


Leu Asn Lys Tyr Ala Pro Glu Leu Ala Asn Leu Pro Thr Thr Asn Gly 
    210                 215                 220                 


Gln Gln Thr Thr Gly Asp Gly Gln Arg Ile Leu Glu Lys Leu Gly Ala 
225                 230                 235                 240 


Asp Leu Ile Asp Met Ser Gln Ile Gln Val His Pro Thr Gly Phe Ile 
                245                 250                 255     


Asp Pro Ala Asn Arg Asp Ser Lys Trp Lys Phe Leu Ala Ala Glu Ala 
            260                 265                 270         


Leu Arg Gly Leu Gly Gly Ile Leu Leu Asn Pro Ser Thr Gly Lys Arg 
        275                 280                 285             


Phe Val Asn Glu Leu Thr Thr Arg Asp Leu Val Thr Glu Ala Ile Gln 
    290                 295                 300                 


Ser Gln Cys Pro Arg Asp Asp Asn Lys Ala Phe Leu Val Met Ser Glu 
305                 310                 315                 320 


Lys Val Tyr Glu Asn Tyr Lys Asn Asn Met Asp Phe Tyr Leu Phe Lys 
                325                 330                 335     


Lys Leu Val Ser Lys Met Thr Ile Lys Glu Phe Val Glu Thr Tyr Lys 
            340                 345                 350         


Leu Pro Ile Ser Ala Asp Ala Val Thr Gln Asp Leu Ile Asp Tyr Ser 
        355                 360                 365             


Val Asp Lys Thr Asp Lys Phe Gly Arg Pro Leu Val Ile Asn Val Phe 
    370                 375                 380                 


Asp Glu Lys Leu Thr Glu Asp Ser Glu Ile Tyr Val Gly Glu Val Thr 
385                 390                 395                 400 


Pro Val Val His Phe Thr Met Gly Gly Ala Lys Ile Asn Thr Glu Ser 
                405                 410                 415     


Gln Val Ile Asn Lys Asn Gly Gln Val Leu Ala Lys Gly Ile Tyr Ala 
            420                 425                 430         


Ala Gly Glu Val Ser Gly Gly Val His Gly Ser Asn Arg Leu Gly Gly 
        435                 440                 445             


Ser Ser Leu Leu Glu Cys Val Val Tyr Gly Arg Ser Ala Ala Asp Asn 
    450                 455                 460                 


Ile Ala Lys Asn Ile Glu 
465                 470 


<210>  35
<211>  1464
<212>  DNA
<213>  Kluyveromyces marxianus


<220>
<221>  CDS
<222>  (1)..(1464)

<400>  35
atg ttg cac aga tac atc cgt ttg ttc tcc ttc tgc gtc atc ttg tac         48
Met Leu His Arg Tyr Ile Arg Leu Phe Ser Phe Cys Val Ile Leu Tyr           
1               5                   10                  15                

tta gtc tat ttg tta ctt act aag gag tca aac gtc atg tct aag cct         96
Leu Val Tyr Leu Leu Leu Thr Lys Glu Ser Asn Val Met Ser Lys Pro           
            20                  25                  30                    

gtt gtt gtt att ggt tct ggt tta gca ggc tta aca aca tct tca caa        144
Val Val Val Ile Gly Ser Gly Leu Ala Gly Leu Thr Thr Ser Ser Gln           
        35                  40                  45                        

tta gca aag ttt aac att cca atc gtc ctt tta gaa aag aca tct tcc        192
Leu Ala Lys Phe Asn Ile Pro Ile Val Leu Leu Glu Lys Thr Ser Ser           
    50                  55                  60                            

att ggt ggt aat tcc att aag gca tct tct ggt atc aat ggc gca ggc        240
Ile Gly Gly Asn Ser Ile Lys Ala Ser Ser Gly Ile Asn Gly Ala Gly           
65                  70                  75                  80            

acc gaa act caa tct cgt tta cac gtt gaa gat cac cca gaa ttg ttt        288
Thr Glu Thr Gln Ser Arg Leu His Val Glu Asp His Pro Glu Leu Phe           
                85                  90                  95                

gct gat gat acc att aag tct gca aaa ggt aaa ggt gtt gtc gct ttg        336
Ala Asp Asp Thr Ile Lys Ser Ala Lys Gly Lys Gly Val Val Ala Leu           
            100                 105                 110                   

atg gaa aag tta tct aaa gac tcc tct gat gct att tcc tgg tta caa        384
Met Glu Lys Leu Ser Lys Asp Ser Ser Asp Ala Ile Ser Trp Leu Gln           
        115                 120                 125                       

aac gac ttc aag att cct ttg gat aag tta gct caa tta ggc ggt cat        432
Asn Asp Phe Lys Ile Pro Leu Asp Lys Leu Ala Gln Leu Gly Gly His           
    130                 135                 140                           

tcc gtt cct aga acc cat aga tca tcc ggc aag ctt cca cca ggt ttc        480
Ser Val Pro Arg Thr His Arg Ser Ser Gly Lys Leu Pro Pro Gly Phe           
145                 150                 155                 160           

caa att gtc gat acc ttg aaa aag gcc ttg gag tct tat gac tct aaa        528
Gln Ile Val Asp Thr Leu Lys Lys Ala Leu Glu Ser Tyr Asp Ser Lys           
                165                 170                 175               

gca gtt aag atc caa ttg aat tct aag gtc gtt gat gtt aag ctt gat        576
Ala Val Lys Ile Gln Leu Asn Ser Lys Val Val Asp Val Lys Leu Asp           
            180                 185                 190                   

tcc aat aac aga gtt tca tct gtt gtt ttc gaa gat caa gat ggt act        624
Ser Asn Asn Arg Val Ser Ser Val Val Phe Glu Asp Gln Asp Gly Thr           
        195                 200                 205                       

cac acc att gaa acc aac aac gtc gtt ttc tgt act ggt ggt ttc ggt        672
His Thr Ile Glu Thr Asn Asn Val Val Phe Cys Thr Gly Gly Phe Gly           
    210                 215                 220                           

ttc aac aaa aag tta ttg gag aag tat gca cca cac ttg gtc gac ttg        720
Phe Asn Lys Lys Leu Leu Glu Lys Tyr Ala Pro His Leu Val Asp Leu           
225                 230                 235                 240           

cca act acc aac ggt gag caa acc tta ggt gaa ggt cag gtc tta ttg        768
Pro Thr Thr Asn Gly Glu Gln Thr Leu Gly Glu Gly Gln Val Leu Leu           
                245                 250                 255               

gaa aaa ctt ggt gct aag ttg att gat atg gac caa att caa gtt cat        816
Glu Lys Leu Gly Ala Lys Leu Ile Asp Met Asp Gln Ile Gln Val His           
            260                 265                 270                   

cca act ggc ttt atc gat cca gcc aat cca gat tct aat tgg aag ttt        864
Pro Thr Gly Phe Ile Asp Pro Ala Asn Pro Asp Ser Asn Trp Lys Phe           
        275                 280                 285                       

ttg gct gcc gag gcc tta aga ggt tta ggt ggt gtc ttg atc aat cca        912
Leu Ala Ala Glu Ala Leu Arg Gly Leu Gly Gly Val Leu Ile Asn Pro           
    290                 295                 300                           

cac act ggt cag aga ttt gtt aac gaa ttg aca act aga gac atg gtc        960
His Thr Gly Gln Arg Phe Val Asn Glu Leu Thr Thr Arg Asp Met Val           
305                 310                 315                 320           

acc gaa gct atc cag tct aag tcc gaa tcc aag act gct tac ttg gtt       1008
Thr Glu Ala Ile Gln Ser Lys Ser Glu Ser Lys Thr Ala Tyr Leu Val           
                325                 330                 335               

atg tcc gag tcc tta tac gag aac tac aag cca aac atg gac ttc tat       1056
Met Ser Glu Ser Leu Tyr Glu Asn Tyr Lys Pro Asn Met Asp Phe Tyr           
            340                 345                 350                   

atg ttc aaa aag ctt gtt tcc aaa aag acc gtt gct gaa ttt gct gaa       1104
Met Phe Lys Lys Leu Val Ser Lys Lys Thr Val Ala Glu Phe Ala Glu           
        355                 360                 365                       

gat ttg cca gtt tct gtt gac caa ctt att gca gaa ctt tca act tat       1152
Asp Leu Pro Val Ser Val Asp Gln Leu Ile Ala Glu Leu Ser Thr Tyr           
    370                 375                 380                           

tcc gac ttg tct aag gat gat cat ttg ggt aga aag ttt aga gaa aac       1200
Ser Asp Leu Ser Lys Asp Asp His Leu Gly Arg Lys Phe Arg Glu Asn           
385                 390                 395                 400           

act ttt ggt tcc tca tta tca tca gac tca acc att ttc gtt ggc aag       1248
Thr Phe Gly Ser Ser Leu Ser Ser Asp Ser Thr Ile Phe Val Gly Lys           
                405                 410                 415               

att act cct gtt gtt cac ttc aca atg ggt ggt gca aag att gat gaa       1296
Ile Thr Pro Val Val His Phe Thr Met Gly Gly Ala Lys Ile Asp Glu           
            420                 425                 430                   

caa gct aga gtc ttg aat gca gaa ggt aaa cca tta gct act ggt atc       1344
Gln Ala Arg Val Leu Asn Ala Glu Gly Lys Pro Leu Ala Thr Gly Ile           
        435                 440                 445                       

tac gcc gct ggt gaa gtt tct ggt ggt gtc cat ggt gct aat aga tta       1392
Tyr Ala Ala Gly Glu Val Ser Gly Gly Val His Gly Ala Asn Arg Leu           
    450                 455                 460                           

ggt ggt tcc tct ttg tta gaa tgt gtt gtc ttt ggt aga caa gca gca       1440
Gly Gly Ser Ser Leu Leu Glu Cys Val Val Phe Gly Arg Gln Ala Ala           
465                 470                 475                 480           

aaa tcc att aga gca aac ttg taa                                       1464
Lys Ser Ile Arg Ala Asn Leu                                               
                485                                                       


<210>  36
<211>  487
<212>  PRT
<213>  Kluyveromyces marxianus

<400>  36

Met Leu His Arg Tyr Ile Arg Leu Phe Ser Phe Cys Val Ile Leu Tyr 
1               5                   10                  15      


Leu Val Tyr Leu Leu Leu Thr Lys Glu Ser Asn Val Met Ser Lys Pro 
            20                  25                  30          


Val Val Val Ile Gly Ser Gly Leu Ala Gly Leu Thr Thr Ser Ser Gln 
        35                  40                  45              


Leu Ala Lys Phe Asn Ile Pro Ile Val Leu Leu Glu Lys Thr Ser Ser 
    50                  55                  60                  


Ile Gly Gly Asn Ser Ile Lys Ala Ser Ser Gly Ile Asn Gly Ala Gly 
65                  70                  75                  80  


Thr Glu Thr Gln Ser Arg Leu His Val Glu Asp His Pro Glu Leu Phe 
                85                  90                  95      


Ala Asp Asp Thr Ile Lys Ser Ala Lys Gly Lys Gly Val Val Ala Leu 
            100                 105                 110         


Met Glu Lys Leu Ser Lys Asp Ser Ser Asp Ala Ile Ser Trp Leu Gln 
        115                 120                 125             


Asn Asp Phe Lys Ile Pro Leu Asp Lys Leu Ala Gln Leu Gly Gly His 
    130                 135                 140                 


Ser Val Pro Arg Thr His Arg Ser Ser Gly Lys Leu Pro Pro Gly Phe 
145                 150                 155                 160 


Gln Ile Val Asp Thr Leu Lys Lys Ala Leu Glu Ser Tyr Asp Ser Lys 
                165                 170                 175     


Ala Val Lys Ile Gln Leu Asn Ser Lys Val Val Asp Val Lys Leu Asp 
            180                 185                 190         


Ser Asn Asn Arg Val Ser Ser Val Val Phe Glu Asp Gln Asp Gly Thr 
        195                 200                 205             


His Thr Ile Glu Thr Asn Asn Val Val Phe Cys Thr Gly Gly Phe Gly 
    210                 215                 220                 


Phe Asn Lys Lys Leu Leu Glu Lys Tyr Ala Pro His Leu Val Asp Leu 
225                 230                 235                 240 


Pro Thr Thr Asn Gly Glu Gln Thr Leu Gly Glu Gly Gln Val Leu Leu 
                245                 250                 255     


Glu Lys Leu Gly Ala Lys Leu Ile Asp Met Asp Gln Ile Gln Val His 
            260                 265                 270         


Pro Thr Gly Phe Ile Asp Pro Ala Asn Pro Asp Ser Asn Trp Lys Phe 
        275                 280                 285             


Leu Ala Ala Glu Ala Leu Arg Gly Leu Gly Gly Val Leu Ile Asn Pro 
    290                 295                 300                 


His Thr Gly Gln Arg Phe Val Asn Glu Leu Thr Thr Arg Asp Met Val 
305                 310                 315                 320 


Thr Glu Ala Ile Gln Ser Lys Ser Glu Ser Lys Thr Ala Tyr Leu Val 
                325                 330                 335     


Met Ser Glu Ser Leu Tyr Glu Asn Tyr Lys Pro Asn Met Asp Phe Tyr 
            340                 345                 350         


Met Phe Lys Lys Leu Val Ser Lys Lys Thr Val Ala Glu Phe Ala Glu 
        355                 360                 365             


Asp Leu Pro Val Ser Val Asp Gln Leu Ile Ala Glu Leu Ser Thr Tyr 
    370                 375                 380                 


Ser Asp Leu Ser Lys Asp Asp His Leu Gly Arg Lys Phe Arg Glu Asn 
385                 390                 395                 400 


Thr Phe Gly Ser Ser Leu Ser Ser Asp Ser Thr Ile Phe Val Gly Lys 
                405                 410                 415     


Ile Thr Pro Val Val His Phe Thr Met Gly Gly Ala Lys Ile Asp Glu 
            420                 425                 430         


Gln Ala Arg Val Leu Asn Ala Glu Gly Lys Pro Leu Ala Thr Gly Ile 
        435                 440                 445             


Tyr Ala Ala Gly Glu Val Ser Gly Gly Val His Gly Ala Asn Arg Leu 
    450                 455                 460                 


Gly Gly Ser Ser Leu Leu Glu Cys Val Val Phe Gly Arg Gln Ala Ala 
465                 470                 475                 480 


Lys Ser Ile Arg Ala Asn Leu 
                485         


<210>  37
<211>  3429
<212>  DNA
<213>  Trypanosoma brucei


<220>
<221>  CDS
<222>  (1)..(3429)

<400>  37
atg gtt gat ggt aga tct tca gct tct att gtt gca gtt gat cca gaa         48
Met Val Asp Gly Arg Ser Ser Ala Ser Ile Val Ala Val Asp Pro Glu           
1               5                   10                  15                

aga gca gca aga gaa aga gat gct gca gct aga gct ttg tta caa gat         96
Arg Ala Ala Arg Glu Arg Asp Ala Ala Ala Arg Ala Leu Leu Gln Asp           
            20                  25                  30                    

tct cca ttg cac act acc atg caa tat gct acc tcc ggt tta gaa ttg        144
Ser Pro Leu His Thr Thr Met Gln Tyr Ala Thr Ser Gly Leu Glu Leu           
        35                  40                  45                        

acc gtc cct tat gca ttg aaa gtt gtt gca tct gcc gac acc ttc gat        192
Thr Val Pro Tyr Ala Leu Lys Val Val Ala Ser Ala Asp Thr Phe Asp           
    50                  55                  60                            

aga gct aag gaa gtt gca gat gaa gtc ctt aga tgt gcc tgg caa ttg        240
Arg Ala Lys Glu Val Ala Asp Glu Val Leu Arg Cys Ala Trp Gln Leu           
65                  70                  75                  80            

gct gat aca gtc ctt aac tcc ttt aac cca aac tct gaa gtc tct ctt        288
Ala Asp Thr Val Leu Asn Ser Phe Asn Pro Asn Ser Glu Val Ser Leu           
                85                  90                  95                

gtt ggt aga ctt cca gtc ggt cag aag cat caa atg tcc gcc cca ctt        336
Val Gly Arg Leu Pro Val Gly Gln Lys His Gln Met Ser Ala Pro Leu           
            100                 105                 110                   

aag aga gtt atg gct tgt tgt caa aga gtt tac aat tcc tct gct ggt        384
Lys Arg Val Met Ala Cys Cys Gln Arg Val Tyr Asn Ser Ser Ala Gly           
        115                 120                 125                       

tgt ttc gac cca tcc acc gcc cca gtt gca aag gct ttg cgt gaa atc        432
Cys Phe Asp Pro Ser Thr Ala Pro Val Ala Lys Ala Leu Arg Glu Ile           
    130                 135                 140                           

gct tta ggc aag gag aga aac aat gcc tgt ttg gag gct tta aca caa        480
Ala Leu Gly Lys Glu Arg Asn Asn Ala Cys Leu Glu Ala Leu Thr Gln           
145                 150                 155                 160           

gca tgc act ttg cca aac tct ttc gtc att gac ttt gaa gca ggt act        528
Ala Cys Thr Leu Pro Asn Ser Phe Val Ile Asp Phe Glu Ala Gly Thr           
                165                 170                 175               

atc tca cgt aaa cat gaa cat gct tca ctt gac tta ggt ggt gtt tca        576
Ile Ser Arg Lys His Glu His Ala Ser Leu Asp Leu Gly Gly Val Ser           
            180                 185                 190                   

aag ggt tac atc gtt gac tat gtt att gat aac att aac gca gct ggt        624
Lys Gly Tyr Ile Val Asp Tyr Val Ile Asp Asn Ile Asn Ala Ala Gly           
        195                 200                 205                       

ttc caa aat gtc ttt ttc gat tgg ggt ggt gat tgt aga gcc tcc ggt        672
Phe Gln Asn Val Phe Phe Asp Trp Gly Gly Asp Cys Arg Ala Ser Gly           
    210                 215                 220                           

atg aat gct aga aat acc cct tgg gtt gtt ggt att act aga cca cca        720
Met Asn Ala Arg Asn Thr Pro Trp Val Val Gly Ile Thr Arg Pro Pro           
225                 230                 235                 240           

tca tta gat atg tta cca aac cca cca aag gaa gca tcc tat atc tct        768
Ser Leu Asp Met Leu Pro Asn Pro Pro Lys Glu Ala Ser Tyr Ile Ser           
                245                 250                 255               

gtt atc tca ttg gac aac gaa gct ttg gca acc tcc ggt gat tac gag        816
Val Ile Ser Leu Asp Asn Glu Ala Leu Ala Thr Ser Gly Asp Tyr Glu           
            260                 265                 270                   

aat ttg atc tac aca gct gat gac aag cct tta act tgt act tac gat        864
Asn Leu Ile Tyr Thr Ala Asp Asp Lys Pro Leu Thr Cys Thr Tyr Asp           
        275                 280                 285                       

tgg aag ggc aag gaa ctt atg aag cca tct caa tca aac att gcc caa        912
Trp Lys Gly Lys Glu Leu Met Lys Pro Ser Gln Ser Asn Ile Ala Gln           
    290                 295                 300                           

gtt tca gtt aag tgc tat tca gca atg tac gct gac gct tta gcc acc        960
Val Ser Val Lys Cys Tyr Ser Ala Met Tyr Ala Asp Ala Leu Ala Thr           
305                 310                 315                 320           

gct tgt ttc atc aaa aga gat cca gcc aag gtt aga caa ttg tta gat       1008
Ala Cys Phe Ile Lys Arg Asp Pro Ala Lys Val Arg Gln Leu Leu Asp           
                325                 330                 335               

ggt tgg aga tac gtt aga gat act gtc aga gat tac aga gtt tat gtt       1056
Gly Trp Arg Tyr Val Arg Asp Thr Val Arg Asp Tyr Arg Val Tyr Val           
            340                 345                 350                   

aga gaa aat gag aga gtc gct aag atg ttt gaa att gca acc gaa gat       1104
Arg Glu Asn Glu Arg Val Ala Lys Met Phe Glu Ile Ala Thr Glu Asp           
        355                 360                 365                       

gct gaa atg aga aaa aga cgt atc tct aat act ttg cct gca aga gtc       1152
Ala Glu Met Arg Lys Arg Arg Ile Ser Asn Thr Leu Pro Ala Arg Val           
    370                 375                 380                           

atc gtt gtc ggt ggc ggt tta gca ggt tta tct gca gca att gaa gct       1200
Ile Val Val Gly Gly Gly Leu Ala Gly Leu Ser Ala Ala Ile Glu Ala           
385                 390                 395                 400           

gca ggc tgc ggt gca caa gtc gtt ttg atg gaa aag gaa gct aag tta       1248
Ala Gly Cys Gly Ala Gln Val Val Leu Met Glu Lys Glu Ala Lys Leu           
                405                 410                 415               

ggt ggt aac tct gca aag gca acc tct ggt atc aat ggt tgg ggt act       1296
Gly Gly Asn Ser Ala Lys Ala Thr Ser Gly Ile Asn Gly Trp Gly Thr           
            420                 425                 430                   

aga gcc caa gca aag gct tcc att gtt gac ggt ggc aag tat ttc gaa       1344
Arg Ala Gln Ala Lys Ala Ser Ile Val Asp Gly Gly Lys Tyr Phe Glu           
        435                 440                 445                       

aga gat act tac aaa tct ggt att ggt ggt aat acc gac cca gct tta       1392
Arg Asp Thr Tyr Lys Ser Gly Ile Gly Gly Asn Thr Asp Pro Ala Leu           
    450                 455                 460                           

gtt aag act ctt tcc atg aag tct gct gac gct att ggt tgg tta aca       1440
Val Lys Thr Leu Ser Met Lys Ser Ala Asp Ala Ile Gly Trp Leu Thr           
465                 470                 475                 480           

tca tta ggt gtt cct tta aca gtc tta tca caa ttg ggt ggt cat tcc       1488
Ser Leu Gly Val Pro Leu Thr Val Leu Ser Gln Leu Gly Gly His Ser           
                485                 490                 495               

aga aag aga act cac aga gca cca gac aaa aag gat ggc acc cca tta       1536
Arg Lys Arg Thr His Arg Ala Pro Asp Lys Lys Asp Gly Thr Pro Leu           
            500                 505                 510                   

cct att ggt ttt acc att atg aaa acc tta gaa gat cac gtc aga ggt       1584
Pro Ile Gly Phe Thr Ile Met Lys Thr Leu Glu Asp His Val Arg Gly           
        515                 520                 525                       

aat ctt tct ggt aga att act atc atg gaa aac tgt tcc gtt acc tct       1632
Asn Leu Ser Gly Arg Ile Thr Ile Met Glu Asn Cys Ser Val Thr Ser           
    530                 535                 540                           

tta ctt tct gaa act aag gaa aga cca gat ggt act aaa caa atc aga       1680
Leu Leu Ser Glu Thr Lys Glu Arg Pro Asp Gly Thr Lys Gln Ile Arg           
545                 550                 555                 560           

gtt acc ggt gtt gag ttc act caa gca ggc tct ggc aaa act acc att       1728
Val Thr Gly Val Glu Phe Thr Gln Ala Gly Ser Gly Lys Thr Thr Ile           
                565                 570                 575               

ttg gcc gac gca gtc atc ttg gcc act ggt ggt ttc tct aac gac aag       1776
Leu Ala Asp Ala Val Ile Leu Ala Thr Gly Gly Phe Ser Asn Asp Lys           
            580                 585                 590                   

acc gca gac tct ttg ttg aga gaa cat gcc cct cac tta gtt aac ttt       1824
Thr Ala Asp Ser Leu Leu Arg Glu His Ala Pro His Leu Val Asn Phe           
        595                 600                 605                       

cct aca act aac ggt cct tgg gca act ggt gac ggt gtt aag ctt gct       1872
Pro Thr Thr Asn Gly Pro Trp Ala Thr Gly Asp Gly Val Lys Leu Ala           
    610                 615                 620                           

caa aga tta ggt gca caa ttg gtc gac atg gat aag gtt caa ttg cat       1920
Gln Arg Leu Gly Ala Gln Leu Val Asp Met Asp Lys Val Gln Leu His           
625                 630                 635                 640           

cca act ggt ttg att aac cca aaa gat cca gct aat cca aca aag ttt       1968
Pro Thr Gly Leu Ile Asn Pro Lys Asp Pro Ala Asn Pro Thr Lys Phe           
                645                 650                 655               

ttg ggt cca gaa gct tta aga ggt tcc ggt ggt gtc ttg tta aac aaa       2016
Leu Gly Pro Glu Ala Leu Arg Gly Ser Gly Gly Val Leu Leu Asn Lys           
            660                 665                 670                   

cag ggt aaa aga ttt gtt aac gaa tta gat ttg cgt tct gtt gtt tcc       2064
Gln Gly Lys Arg Phe Val Asn Glu Leu Asp Leu Arg Ser Val Val Ser           
        675                 680                 685                       

aag gcc att atg gaa caa ggt gct gaa tac cca ggc tct ggt ggt tct       2112
Lys Ala Ile Met Glu Gln Gly Ala Glu Tyr Pro Gly Ser Gly Gly Ser           
    690                 695                 700                           

atg ttc gca tat tgt gtc ctt aat gca gct gca caa aag ttg ttt ggt       2160
Met Phe Ala Tyr Cys Val Leu Asn Ala Ala Ala Gln Lys Leu Phe Gly           
705                 710                 715                 720           

gtc tct tcc cac gag ttc tac tgg aaa aag atg ggt ttg ttc gtt aag       2208
Val Ser Ser His Glu Phe Tyr Trp Lys Lys Met Gly Leu Phe Val Lys           
                725                 730                 735               

gct gat act atg aga gat ttg gca gca ttg att ggt tgt cca gtc gag       2256
Ala Asp Thr Met Arg Asp Leu Ala Ala Leu Ile Gly Cys Pro Val Glu           
            740                 745                 750                   

tct gtt caa caa act tta gag gaa tat gaa aga tta tct att tct cag       2304
Ser Val Gln Gln Thr Leu Glu Glu Tyr Glu Arg Leu Ser Ile Ser Gln           
        755                 760                 765                       

aga tcc tgt cca atc act aga aaa tct gtt tac cca tgt gtt ttg ggc       2352
Arg Ser Cys Pro Ile Thr Arg Lys Ser Val Tyr Pro Cys Val Leu Gly           
    770                 775                 780                           

act aag ggt cca tac tac gtt gct ttc gtc acc cca tct att cac tat       2400
Thr Lys Gly Pro Tyr Tyr Val Ala Phe Val Thr Pro Ser Ile His Tyr           
785                 790                 795                 800           

aca atg ggt ggt tgt ttg att tcc cca tca gca gaa att cag atg aaa       2448
Thr Met Gly Gly Cys Leu Ile Ser Pro Ser Ala Glu Ile Gln Met Lys           
                805                 810                 815               

aac acc tcc tcc cgt gct cca ttg tcc cat tcc aac cct atc ttg ggt       2496
Asn Thr Ser Ser Arg Ala Pro Leu Ser His Ser Asn Pro Ile Leu Gly           
            820                 825                 830                   

ttg ttc ggt gct ggt gaa gtt act ggt ggt gtc cac ggt ggc aat aga       2544
Leu Phe Gly Ala Gly Glu Val Thr Gly Gly Val His Gly Gly Asn Arg           
        835                 840                 845                       

tta ggt ggt aac tca ttg tta gaa tgt gtt gtc ttt ggt aga att gct       2592
Leu Gly Gly Asn Ser Leu Leu Glu Cys Val Val Phe Gly Arg Ile Ala           
    850                 855                 860                           

ggt gat aga gct tct acc att ttg cag aga aag tcc tcc gca tta tct       2640
Gly Asp Arg Ala Ser Thr Ile Leu Gln Arg Lys Ser Ser Ala Leu Ser           
865                 870                 875                 880           

ttc aag gtc tgg act acc gtt gtt ttg aga gaa gtt aga gaa ggt ggc       2688
Phe Lys Val Trp Thr Thr Val Val Leu Arg Glu Val Arg Glu Gly Gly           
                885                 890                 895               

gtc tat ggt gcc ggt tca aga gtt ttg aga ttc aac ttg cct ggt gct       2736
Val Tyr Gly Ala Gly Ser Arg Val Leu Arg Phe Asn Leu Pro Gly Ala           
            900                 905                 910                   

tta caa aga tcc ggt ttg tcc ttg ggt caa ttc atc gca atc aga ggt       2784
Leu Gln Arg Ser Gly Leu Ser Leu Gly Gln Phe Ile Ala Ile Arg Gly           
        915                 920                 925                       

gac tgg gat ggt caa caa ttg att ggt tac tat tcc cca att aca ttg       2832
Asp Trp Asp Gly Gln Gln Leu Ile Gly Tyr Tyr Ser Pro Ile Thr Leu           
    930                 935                 940                           

cca gat gac ttg ggt atg att gac att ttg gct aga tcc gat aaa ggt       2880
Pro Asp Asp Leu Gly Met Ile Asp Ile Leu Ala Arg Ser Asp Lys Gly           
945                 950                 955                 960           

act tta aga gaa tgg att tct gct tta gaa cca ggc gac gct gtt gag       2928
Thr Leu Arg Glu Trp Ile Ser Ala Leu Glu Pro Gly Asp Ala Val Glu           
                965                 970                 975               

atg aaa gca tgc ggt ggt tta gtc atc gag aga aga ttg tca gat aag       2976
Met Lys Ala Cys Gly Gly Leu Val Ile Glu Arg Arg Leu Ser Asp Lys           
            980                 985                 990                   

cac ttt gtc ttt atg ggt cac atc  att aac aag tta tgt  ttg atc gct     3024
His Phe Val Phe Met Gly His Ile  Ile Asn Lys Leu Cys  Leu Ile Ala         
        995                 1000                 1005                     

ggt ggt  aca ggc gtt gca cct  atg tta caa atc att  aag gca gca        3069
Gly Gly  Thr Gly Val Ala Pro  Met Leu Gln Ile Ile  Lys Ala Ala            
    1010                 1015                 1020                        

ttc atg  aaa cct ttt atc gat  acc tta gaa tct gtc  cat ctt atc        3114
Phe Met  Lys Pro Phe Ile Asp  Thr Leu Glu Ser Val  His Leu Ile            
    1025                 1030                 1035                        

tat gct  gca gaa gat gtt acc  gag tta act tat aga  gaa gtt tta        3159
Tyr Ala  Ala Glu Asp Val Thr  Glu Leu Thr Tyr Arg  Glu Val Leu            
    1040                 1045                 1050                        

gag gag  cgt aga aga gag tct  cgt ggc aag ttc aaa  aag acc ttt        3204
Glu Glu  Arg Arg Arg Glu Ser  Arg Gly Lys Phe Lys  Lys Thr Phe            
    1055                 1060                 1065                        

gtt ttg  aac aga cct cca cca  ctt tgg act gat ggt  gtt ggt ttc        3249
Val Leu  Asn Arg Pro Pro Pro  Leu Trp Thr Asp Gly  Val Gly Phe            
    1070                 1075                 1080                        

atc gat  aga ggt atc tta act  aat cat gtc caa cca  cca tcc gat        3294
Ile Asp  Arg Gly Ile Leu Thr  Asn His Val Gln Pro  Pro Ser Asp            
    1085                 1090                 1095                        

aac ctt  ttg gtt gca atc tgt  ggt cca cct gtc atg  cag cgt att        3339
Asn Leu  Leu Val Ala Ile Cys  Gly Pro Pro Val Met  Gln Arg Ile            
    1100                 1105                 1110                        

gtt aag  gcc acc tta aag act  ttg ggt tac aat atg  aat ctt gtt        3384
Val Lys  Ala Thr Leu Lys Thr  Leu Gly Tyr Asn Met  Asn Leu Val            
    1115                 1120                 1125                        

aga aca  gtt gac gaa aca gaa  cca tcc ggt tcc taa tta  att aac        3429
Arg Thr  Val Asp Glu Thr Glu  Pro Ser Gly Ser     Leu  Ile Asn            
    1130                 1135                     1140                    


<210>  38
<211>  1139
<212>  PRT
<213>  Trypanosoma brucei

<400>  38

Met Val Asp Gly Arg Ser Ser Ala Ser Ile Val Ala Val Asp Pro Glu 
1               5                   10                  15      


Arg Ala Ala Arg Glu Arg Asp Ala Ala Ala Arg Ala Leu Leu Gln Asp 
            20                  25                  30          


Ser Pro Leu His Thr Thr Met Gln Tyr Ala Thr Ser Gly Leu Glu Leu 
        35                  40                  45              


Thr Val Pro Tyr Ala Leu Lys Val Val Ala Ser Ala Asp Thr Phe Asp 
    50                  55                  60                  


Arg Ala Lys Glu Val Ala Asp Glu Val Leu Arg Cys Ala Trp Gln Leu 
65                  70                  75                  80  


Ala Asp Thr Val Leu Asn Ser Phe Asn Pro Asn Ser Glu Val Ser Leu 
                85                  90                  95      


Val Gly Arg Leu Pro Val Gly Gln Lys His Gln Met Ser Ala Pro Leu 
            100                 105                 110         


Lys Arg Val Met Ala Cys Cys Gln Arg Val Tyr Asn Ser Ser Ala Gly 
        115                 120                 125             


Cys Phe Asp Pro Ser Thr Ala Pro Val Ala Lys Ala Leu Arg Glu Ile 
    130                 135                 140                 


Ala Leu Gly Lys Glu Arg Asn Asn Ala Cys Leu Glu Ala Leu Thr Gln 
145                 150                 155                 160 


Ala Cys Thr Leu Pro Asn Ser Phe Val Ile Asp Phe Glu Ala Gly Thr 
                165                 170                 175     


Ile Ser Arg Lys His Glu His Ala Ser Leu Asp Leu Gly Gly Val Ser 
            180                 185                 190         


Lys Gly Tyr Ile Val Asp Tyr Val Ile Asp Asn Ile Asn Ala Ala Gly 
        195                 200                 205             


Phe Gln Asn Val Phe Phe Asp Trp Gly Gly Asp Cys Arg Ala Ser Gly 
    210                 215                 220                 


Met Asn Ala Arg Asn Thr Pro Trp Val Val Gly Ile Thr Arg Pro Pro 
225                 230                 235                 240 


Ser Leu Asp Met Leu Pro Asn Pro Pro Lys Glu Ala Ser Tyr Ile Ser 
                245                 250                 255     


Val Ile Ser Leu Asp Asn Glu Ala Leu Ala Thr Ser Gly Asp Tyr Glu 
            260                 265                 270         


Asn Leu Ile Tyr Thr Ala Asp Asp Lys Pro Leu Thr Cys Thr Tyr Asp 
        275                 280                 285             


Trp Lys Gly Lys Glu Leu Met Lys Pro Ser Gln Ser Asn Ile Ala Gln 
    290                 295                 300                 


Val Ser Val Lys Cys Tyr Ser Ala Met Tyr Ala Asp Ala Leu Ala Thr 
305                 310                 315                 320 


Ala Cys Phe Ile Lys Arg Asp Pro Ala Lys Val Arg Gln Leu Leu Asp 
                325                 330                 335     


Gly Trp Arg Tyr Val Arg Asp Thr Val Arg Asp Tyr Arg Val Tyr Val 
            340                 345                 350         


Arg Glu Asn Glu Arg Val Ala Lys Met Phe Glu Ile Ala Thr Glu Asp 
        355                 360                 365             


Ala Glu Met Arg Lys Arg Arg Ile Ser Asn Thr Leu Pro Ala Arg Val 
    370                 375                 380                 


Ile Val Val Gly Gly Gly Leu Ala Gly Leu Ser Ala Ala Ile Glu Ala 
385                 390                 395                 400 


Ala Gly Cys Gly Ala Gln Val Val Leu Met Glu Lys Glu Ala Lys Leu 
                405                 410                 415     


Gly Gly Asn Ser Ala Lys Ala Thr Ser Gly Ile Asn Gly Trp Gly Thr 
            420                 425                 430         


Arg Ala Gln Ala Lys Ala Ser Ile Val Asp Gly Gly Lys Tyr Phe Glu 
        435                 440                 445             


Arg Asp Thr Tyr Lys Ser Gly Ile Gly Gly Asn Thr Asp Pro Ala Leu 
    450                 455                 460                 


Val Lys Thr Leu Ser Met Lys Ser Ala Asp Ala Ile Gly Trp Leu Thr 
465                 470                 475                 480 


Ser Leu Gly Val Pro Leu Thr Val Leu Ser Gln Leu Gly Gly His Ser 
                485                 490                 495     


Arg Lys Arg Thr His Arg Ala Pro Asp Lys Lys Asp Gly Thr Pro Leu 
            500                 505                 510         


Pro Ile Gly Phe Thr Ile Met Lys Thr Leu Glu Asp His Val Arg Gly 
        515                 520                 525             


Asn Leu Ser Gly Arg Ile Thr Ile Met Glu Asn Cys Ser Val Thr Ser 
    530                 535                 540                 


Leu Leu Ser Glu Thr Lys Glu Arg Pro Asp Gly Thr Lys Gln Ile Arg 
545                 550                 555                 560 


Val Thr Gly Val Glu Phe Thr Gln Ala Gly Ser Gly Lys Thr Thr Ile 
                565                 570                 575     


Leu Ala Asp Ala Val Ile Leu Ala Thr Gly Gly Phe Ser Asn Asp Lys 
            580                 585                 590         


Thr Ala Asp Ser Leu Leu Arg Glu His Ala Pro His Leu Val Asn Phe 
        595                 600                 605             


Pro Thr Thr Asn Gly Pro Trp Ala Thr Gly Asp Gly Val Lys Leu Ala 
    610                 615                 620                 


Gln Arg Leu Gly Ala Gln Leu Val Asp Met Asp Lys Val Gln Leu His 
625                 630                 635                 640 


Pro Thr Gly Leu Ile Asn Pro Lys Asp Pro Ala Asn Pro Thr Lys Phe 
                645                 650                 655     


Leu Gly Pro Glu Ala Leu Arg Gly Ser Gly Gly Val Leu Leu Asn Lys 
            660                 665                 670         


Gln Gly Lys Arg Phe Val Asn Glu Leu Asp Leu Arg Ser Val Val Ser 
        675                 680                 685             


Lys Ala Ile Met Glu Gln Gly Ala Glu Tyr Pro Gly Ser Gly Gly Ser 
    690                 695                 700                 


Met Phe Ala Tyr Cys Val Leu Asn Ala Ala Ala Gln Lys Leu Phe Gly 
705                 710                 715                 720 


Val Ser Ser His Glu Phe Tyr Trp Lys Lys Met Gly Leu Phe Val Lys 
                725                 730                 735     


Ala Asp Thr Met Arg Asp Leu Ala Ala Leu Ile Gly Cys Pro Val Glu 
            740                 745                 750         


Ser Val Gln Gln Thr Leu Glu Glu Tyr Glu Arg Leu Ser Ile Ser Gln 
        755                 760                 765             


Arg Ser Cys Pro Ile Thr Arg Lys Ser Val Tyr Pro Cys Val Leu Gly 
    770                 775                 780                 


Thr Lys Gly Pro Tyr Tyr Val Ala Phe Val Thr Pro Ser Ile His Tyr 
785                 790                 795                 800 


Thr Met Gly Gly Cys Leu Ile Ser Pro Ser Ala Glu Ile Gln Met Lys 
                805                 810                 815     


Asn Thr Ser Ser Arg Ala Pro Leu Ser His Ser Asn Pro Ile Leu Gly 
            820                 825                 830         


Leu Phe Gly Ala Gly Glu Val Thr Gly Gly Val His Gly Gly Asn Arg 
        835                 840                 845             


Leu Gly Gly Asn Ser Leu Leu Glu Cys Val Val Phe Gly Arg Ile Ala 
    850                 855                 860                 


Gly Asp Arg Ala Ser Thr Ile Leu Gln Arg Lys Ser Ser Ala Leu Ser 
865                 870                 875                 880 


Phe Lys Val Trp Thr Thr Val Val Leu Arg Glu Val Arg Glu Gly Gly 
                885                 890                 895     


Val Tyr Gly Ala Gly Ser Arg Val Leu Arg Phe Asn Leu Pro Gly Ala 
            900                 905                 910         


Leu Gln Arg Ser Gly Leu Ser Leu Gly Gln Phe Ile Ala Ile Arg Gly 
        915                 920                 925             


Asp Trp Asp Gly Gln Gln Leu Ile Gly Tyr Tyr Ser Pro Ile Thr Leu 
    930                 935                 940                 


Pro Asp Asp Leu Gly Met Ile Asp Ile Leu Ala Arg Ser Asp Lys Gly 
945                 950                 955                 960 


Thr Leu Arg Glu Trp Ile Ser Ala Leu Glu Pro Gly Asp Ala Val Glu 
                965                 970                 975     


Met Lys Ala Cys Gly Gly Leu Val Ile Glu Arg Arg Leu Ser Asp Lys 
            980                 985                 990         


His Phe Val Phe Met Gly His Ile  Ile Asn Lys Leu Cys  Leu Ile Ala 
        995                 1000                 1005             


Gly Gly  Thr Gly Val Ala Pro  Met Leu Gln Ile Ile  Lys Ala Ala 
    1010                 1015                 1020             


Phe Met  Lys Pro Phe Ile Asp  Thr Leu Glu Ser Val  His Leu Ile 
    1025                 1030                 1035             


Tyr Ala  Ala Glu Asp Val Thr  Glu Leu Thr Tyr Arg  Glu Val Leu 
    1040                 1045                 1050             


Glu Glu  Arg Arg Arg Glu Ser  Arg Gly Lys Phe Lys  Lys Thr Phe 
    1055                 1060                 1065             


Val Leu  Asn Arg Pro Pro Pro  Leu Trp Thr Asp Gly  Val Gly Phe 
    1070                 1075                 1080             


Ile Asp  Arg Gly Ile Leu Thr  Asn His Val Gln Pro  Pro Ser Asp 
    1085                 1090                 1095             


Asn Leu  Leu Val Ala Ile Cys  Gly Pro Pro Val Met  Gln Arg Ile 
    1100                 1105                 1110             


Val Lys  Ala Thr Leu Lys Thr  Leu Gly Tyr Asn Met  Asn Leu Val 
    1115                 1120                 1125             


Arg Thr  Val Asp Glu Thr Glu  Pro Ser Gly Ser 
    1130                 1135                 


<210>  39
<211>  3420
<212>  DNA
<213>  Trypanosoma cruzi


<220>
<221>  CDS
<222>  (1)..(3420)

<400>  39
atg gct gac ggt aga tcc tct gca tct gtt gtt gca gtt gat cca gaa         48
Met Ala Asp Gly Arg Ser Ser Ala Ser Val Val Ala Val Asp Pro Glu           
1               5                   10                  15                

aag gct gca aga gaa aga gat gaa gca gct cgt gct ttg tta aga gac         96
Lys Ala Ala Arg Glu Arg Asp Glu Ala Ala Arg Ala Leu Leu Arg Asp           
            20                  25                  30                    

tct cca tta caa act cat ctt cag tac atg act aat ggt tta gag ttg        144
Ser Pro Leu Gln Thr His Leu Gln Tyr Met Thr Asn Gly Leu Glu Leu           
        35                  40                  45                        

act gtc cca ttc acc tta aag gtt gtc gct gaa gca gtt gca ttt tcc        192
Thr Val Pro Phe Thr Leu Lys Val Val Ala Glu Ala Val Ala Phe Ser           
    50                  55                  60                            

aga gca aag gaa gtt gct gac gaa gtt ttg agg tca gcc tgg cat ctt        240
Arg Ala Lys Glu Val Ala Asp Glu Val Leu Arg Ser Ala Trp His Leu           
65                  70                  75                  80            

gca gac acc gtc ttg aac aac ttt aac cct aac tcc gag att tct atg        288
Ala Asp Thr Val Leu Asn Asn Phe Asn Pro Asn Ser Glu Ile Ser Met           
                85                  90                  95                

att ggt aga tta cca gtt ggt caa aaa cat aca atg tcc gct aca ttg        336
Ile Gly Arg Leu Pro Val Gly Gln Lys His Thr Met Ser Ala Thr Leu           
            100                 105                 110                   

aag tct gtt atc aca tgc tgt cag cat gtt ttc aat tca tcc aga ggt        384
Lys Ser Val Ile Thr Cys Cys Gln His Val Phe Asn Ser Ser Arg Gly           
        115                 120                 125                       

gtt ttt gat cca gct act ggt cct atc att gaa gct tta aga gct aag        432
Val Phe Asp Pro Ala Thr Gly Pro Ile Ile Glu Ala Leu Arg Ala Lys           
    130                 135                 140                           

gtt gct gag aaa gcc tct gtt tct gat gaa cag atg gag aag ttg ttt        480
Val Ala Glu Lys Ala Ser Val Ser Asp Glu Gln Met Glu Lys Leu Phe           
145                 150                 155                 160           

cgt gtt tgt aac ttc tct tcc tca ttc atc gtt gat ttg gaa atg ggt        528
Arg Val Cys Asn Phe Ser Ser Ser Phe Ile Val Asp Leu Glu Met Gly           
                165                 170                 175               

act att gcc aga aaa cac gaa gat gca aga ttt gac tta ggt ggt gtt        576
Thr Ile Ala Arg Lys His Glu Asp Ala Arg Phe Asp Leu Gly Gly Val           
            180                 185                 190                   

tcc aag ggt tac atc gtt gac tac gtt gtt gaa aga ttg aac gct gct        624
Ser Lys Gly Tyr Ile Val Asp Tyr Val Val Glu Arg Leu Asn Ala Ala           
        195                 200                 205                       

ggt att gtc gat gtc tac ttc gaa tgg ggt ggt gac tgt aga gct tcc        672
Gly Ile Val Asp Val Tyr Phe Glu Trp Gly Gly Asp Cys Arg Ala Ser           
    210                 215                 220                           

ggt act aac gca aga cgt acc cca tgg atg gtt ggt atc att aga cct        720
Gly Thr Asn Ala Arg Arg Thr Pro Trp Met Val Gly Ile Ile Arg Pro           
225                 230                 235                 240           

cca tct tta gaa caa ttg aga aac cca cca aaa gat cca tcc tac att        768
Pro Ser Leu Glu Gln Leu Arg Asn Pro Pro Lys Asp Pro Ser Tyr Ile           
                245                 250                 255               

agg gtt tta cca ctt aac gat gaa gca ctt tgt acc tct ggt gac tat        816
Arg Val Leu Pro Leu Asn Asp Glu Ala Leu Cys Thr Ser Gly Asp Tyr           
            260                 265                 270                   

gag aat ttg acc gaa ggc tct aac aaa aag ttg tat aca tcc att ttc        864
Glu Asn Leu Thr Glu Gly Ser Asn Lys Lys Leu Tyr Thr Ser Ile Phe           
        275                 280                 285                       

gat tgg aaa aag aga tcc ttg ttg gaa cca gtt gaa tca gaa ttg gcc        912
Asp Trp Lys Lys Arg Ser Leu Leu Glu Pro Val Glu Ser Glu Leu Ala           
    290                 295                 300                           

caa gtt tcc att aga tgt tat tct gcc atg tat gca gac gca tta gca        960
Gln Val Ser Ile Arg Cys Tyr Ser Ala Met Tyr Ala Asp Ala Leu Ala           
305                 310                 315                 320           

aca gct tct ctt atc aag aga gat atc aaa aag gtt aga caa atg ttg       1008
Thr Ala Ser Leu Ile Lys Arg Asp Ile Lys Lys Val Arg Gln Met Leu           
                325                 330                 335               

gaa gat tgg aga cac gtc cgt aat agg gtt act aac tat gtt acc tat       1056
Glu Asp Trp Arg His Val Arg Asn Arg Val Thr Asn Tyr Val Thr Tyr           
            340                 345                 350                   

acc aga caa ggt gaa aga gtc gca cgt atg ttt gaa att gct act gat       1104
Thr Arg Gln Gly Glu Arg Val Ala Arg Met Phe Glu Ile Ala Thr Asp           
        355                 360                 365                       

aac gct gag att agg aaa aag aga att gca ggc tct tta cct gct agg       1152
Asn Ala Glu Ile Arg Lys Lys Arg Ile Ala Gly Ser Leu Pro Ala Arg           
    370                 375                 380                           

gtt att gtt gtc ggt ggt ggt tta gct ggt ttg tct gca gca att gaa       1200
Val Ile Val Val Gly Gly Gly Leu Ala Gly Leu Ser Ala Ala Ile Glu           
385                 390                 395                 400           

gca act gca tgt ggt gcc caa gtt atc ctt tta gaa aag gaa cct aaa       1248
Ala Thr Ala Cys Gly Ala Gln Val Ile Leu Leu Glu Lys Glu Pro Lys           
                405                 410                 415               

gtt ggt ggt aat tcc gca aag gct aca tct ggt atc aac ggt tgg ggt       1296
Val Gly Gly Asn Ser Ala Lys Ala Thr Ser Gly Ile Asn Gly Trp Gly           
            420                 425                 430                   

act aga gca caa gct gaa caa gat gtc tac gac tct ggc aag tac ttc       1344
Thr Arg Ala Gln Ala Glu Gln Asp Val Tyr Asp Ser Gly Lys Tyr Phe           
        435                 440                 445                       

gaa aga gat aca cac aaa tct ggt tta ggt ggt tct acc gat cca ggc       1392
Glu Arg Asp Thr His Lys Ser Gly Leu Gly Gly Ser Thr Asp Pro Gly           
    450                 455                 460                           

tta gtt cgt act tta tca gtc aag tct ggt gac gct att tca tgg tta       1440
Leu Val Arg Thr Leu Ser Val Lys Ser Gly Asp Ala Ile Ser Trp Leu           
465                 470                 475                 480           

tct tct ctt ggt gtt cca tta act gtc ttg tca caa tta ggc ggt cat       1488
Ser Ser Leu Gly Val Pro Leu Thr Val Leu Ser Gln Leu Gly Gly His           
                485                 490                 495               

tcc aga aaa agg act cac aga gcc cct gat aag gca gat ggt act cca       1536
Ser Arg Lys Arg Thr His Arg Ala Pro Asp Lys Ala Asp Gly Thr Pro           
            500                 505                 510                   

gtt cca att ggt ttc acc att atg caa acc tta gaa cag cat gtt aga       1584
Val Pro Ile Gly Phe Thr Ile Met Gln Thr Leu Glu Gln His Val Arg           
        515                 520                 525                       

acc aag tta gca gac aga gtt act atc atg gag aat acc acc gtt acc       1632
Thr Lys Leu Ala Asp Arg Val Thr Ile Met Glu Asn Thr Thr Val Thr           
    530                 535                 540                           

tcc ttg ctt tct aag tcc aga gtt aga cat gat ggt gca aag caa gtt       1680
Ser Leu Leu Ser Lys Ser Arg Val Arg His Asp Gly Ala Lys Gln Val           
545                 550                 555                 560           

aga gtc tac ggt gtt gaa gtc tta caa gac gaa ggt gtc gtt tct cgt       1728
Arg Val Tyr Gly Val Glu Val Leu Gln Asp Glu Gly Val Val Ser Arg           
                565                 570                 575               

atc ttg gcc gat gct gtc att ttg gca aca ggt ggt ttc tcc aat gac       1776
Ile Leu Ala Asp Ala Val Ile Leu Ala Thr Gly Gly Phe Ser Asn Asp           
            580                 585                 590                   

aaa acc cca aac tcc tta ttg caa gag ttc gct cca caa ttg tca ggt       1824
Lys Thr Pro Asn Ser Leu Leu Gln Glu Phe Ala Pro Gln Leu Ser Gly           
        595                 600                 605                       

ttt cca aca acc aac ggt cca tgg gct act ggc gat ggt gtt aag tta       1872
Phe Pro Thr Thr Asn Gly Pro Trp Ala Thr Gly Asp Gly Val Lys Leu           
    610                 615                 620                           

gca aga gaa ctt ggt gtc aag ttg gtt gat atg gat aag gtc caa ctt       1920
Ala Arg Glu Leu Gly Val Lys Leu Val Asp Met Asp Lys Val Gln Leu           
625                 630                 635                 640           

cat cca act ggt ttg att gac cct aag gac cca gca aat cca acc aaa       1968
His Pro Thr Gly Leu Ile Asp Pro Lys Asp Pro Ala Asn Pro Thr Lys           
                645                 650                 655               

tac tta ggt cca gaa gca ttg aga ggt tct ggt ggt gtc ttg tta aac       2016
Tyr Leu Gly Pro Glu Ala Leu Arg Gly Ser Gly Gly Val Leu Leu Asn           
            660                 665                 670                   

aaa aag ggt gaa aga ttt gtc aat gag ttg gac ttg cgt tcc gtc gtt       2064
Lys Lys Gly Glu Arg Phe Val Asn Glu Leu Asp Leu Arg Ser Val Val           
        675                 680                 685                       

tca aat gct atc att gaa caa ggt gat gaa tat cca gat gcc ggt ggt       2112
Ser Asn Ala Ile Ile Glu Gln Gly Asp Glu Tyr Pro Asp Ala Gly Gly           
    690                 695                 700                           

tcc aag ttc gcc ttc tgt gtt ttg aat gat gca gca gtt aag tta ttc       2160
Ser Lys Phe Ala Phe Cys Val Leu Asn Asp Ala Ala Val Lys Leu Phe           
705                 710                 715                 720           

ggt gtc aac tcc cac ggt ttc tac tgg aag aga ctt ggt ttg ttt gtt       2208
Gly Val Asn Ser His Gly Phe Tyr Trp Lys Arg Leu Gly Leu Phe Val           
                725                 730                 735               

aag gct gat acc gtt gaa aag tta gcc gca ttg atc ggt tgc cca gtc       2256
Lys Ala Asp Thr Val Glu Lys Leu Ala Ala Leu Ile Gly Cys Pro Val           
            740                 745                 750                   

gaa aat gtt aga aac aca tta ggt gat tat gag caa ttg tcc aag gaa       2304
Glu Asn Val Arg Asn Thr Leu Gly Asp Tyr Glu Gln Leu Ser Lys Glu           
        755                 760                 765                       

aac aga caa tgt cca aag act aga aaa gtt gtc tat cca tgt gtt gtt       2352
Asn Arg Gln Cys Pro Lys Thr Arg Lys Val Val Tyr Pro Cys Val Val           
    770                 775                 780                           

ggt cca caa ggt cca ttc tat gtt gct ttt gtt acc cca tct att cac       2400
Gly Pro Gln Gly Pro Phe Tyr Val Ala Phe Val Thr Pro Ser Ile His           
785                 790                 795                 800           

tat acc atg ggt ggt tgt ttg atc tca cca tct gct gag atg caa ttg       2448
Tyr Thr Met Gly Gly Cys Leu Ile Ser Pro Ser Ala Glu Met Gln Leu           
                805                 810                 815               

gaa gag aac act acc tcc cca ttt ggt cac aga agg cct atc ttc ggt       2496
Glu Glu Asn Thr Thr Ser Pro Phe Gly His Arg Arg Pro Ile Phe Gly           
            820                 825                 830                   

ctt ttc ggt gcc ggt gaa gtt act ggt ggt gtc cat ggt ggt aac aga       2544
Leu Phe Gly Ala Gly Glu Val Thr Gly Gly Val His Gly Gly Asn Arg           
        835                 840                 845                       

tta ggt ggc aac tct ttg ttg gag tgt gtt gtt ttt ggt aga atc gct       2592
Leu Gly Gly Asn Ser Leu Leu Glu Cys Val Val Phe Gly Arg Ile Ala           
    850                 855                 860                           

ggt gat aga gct gca acc att ttg caa aag aaa cca gtt cca ctt tcc       2640
Gly Asp Arg Ala Ala Thr Ile Leu Gln Lys Lys Pro Val Pro Leu Ser           
865                 870                 875                 880           

ttt aag act tgg acc acc gtc att ttg aga gag gtc cgt gaa ggt ggc       2688
Phe Lys Thr Trp Thr Thr Val Ile Leu Arg Glu Val Arg Glu Gly Gly           
                885                 890                 895               

atg tac ggt act ggt tca aga gtc tta aga ttc aat ttg cca ggt gct       2736
Met Tyr Gly Thr Gly Ser Arg Val Leu Arg Phe Asn Leu Pro Gly Ala           
            900                 905                 910                   

tta caa aga tct ggt ttg caa ttg ggt caa ttc atc gct att aga ggc       2784
Leu Gln Arg Ser Gly Leu Gln Leu Gly Gln Phe Ile Ala Ile Arg Gly           
        915                 920                 925                       

gaa tgg gat ggt caa caa ttg att ggc tac tat tcc cca atc act ttg       2832
Glu Trp Asp Gly Gln Gln Leu Ile Gly Tyr Tyr Ser Pro Ile Thr Leu           
    930                 935                 940                           

cca gac gat ttg ggt gtc atc ggc att ttg gct aga tcc gat aag ggt       2880
Pro Asp Asp Leu Gly Val Ile Gly Ile Leu Ala Arg Ser Asp Lys Gly           
945                 950                 955                 960           

act ttg aag gaa tgg att tct gct ttg gaa cct ggt gat gca gtt gag       2928
Thr Leu Lys Glu Trp Ile Ser Ala Leu Glu Pro Gly Asp Ala Val Glu           
                965                 970                 975               

atg aag ggt tgt ggc ggt tta gtt att gaa agg aga ttc tct gaa aga       2976
Met Lys Gly Cys Gly Gly Leu Val Ile Glu Arg Arg Phe Ser Glu Arg           
            980                 985                 990                   

tac ttg tac ttt tct ggt cac gct  ttg aaa aag tta tgc  ctt att gct     3024
Tyr Leu Tyr Phe Ser Gly His Ala  Leu Lys Lys Leu Cys  Leu Ile Ala         
        995                 1000                 1005                     

ggt ggt  act ggt gtc gca cca  atg tta caa atc att  aga gca gca        3069
Gly Gly  Thr Gly Val Ala Pro  Met Leu Gln Ile Ile  Arg Ala Ala            
    1010                 1015                 1020                        

ttg aaa  aag cca ttc ctt gag  aat atc gaa tca att  aga ctt atc        3114
Leu Lys  Lys Pro Phe Leu Glu  Asn Ile Glu Ser Ile  Arg Leu Ile            
    1025                 1030                 1035                        

tat gct  gct gag gac gtt tct  gag ttg aca tac agg  gaa ttg tta        3159
Tyr Ala  Ala Glu Asp Val Ser  Glu Leu Thr Tyr Arg  Glu Leu Leu            
    1040                 1045                 1050                        

gaa cat  cac caa aga gat tct  aag ggc aag ttt aga  tcc atc ttc        3204
Glu His  His Gln Arg Asp Ser  Lys Gly Lys Phe Arg  Ser Ile Phe            
    1055                 1060                 1065                        

gtt ttg  aat aga cca cct cca  att tgg act gat ggt  gtt ggc ttt        3249
Val Leu  Asn Arg Pro Pro Pro  Ile Trp Thr Asp Gly  Val Gly Phe            
    1070                 1075                 1080                        

atc gac  aaa aag ttg tta tct  tca tcc gtt cag cca  cct gct aag        3294
Ile Asp  Lys Lys Leu Leu Ser  Ser Ser Val Gln Pro  Pro Ala Lys            
    1085                 1090                 1095                        

gat ttg  tta gtc gcc att tgt  ggt cct cct atc atg  caa cgt gtt        3339
Asp Leu  Leu Val Ala Ile Cys  Gly Pro Pro Ile Met  Gln Arg Val            
    1100                 1105                 1110                        

gtc aag  act tgt ctt aag tca  tta ggt tat gat atg  cag tta gtc        3384
Val Lys  Thr Cys Leu Lys Ser  Leu Gly Tyr Asp Met  Gln Leu Val            
    1115                 1120                 1125                        

aga aca  gtt gat gaa gtc gaa  act caa aac tcc taa                     3420
Arg Thr  Val Asp Glu Val Glu  Thr Gln Asn Ser                             
    1130                 1135                                             


<210>  40
<211>  1139
<212>  PRT
<213>  Trypanosoma cruzi

<400>  40

Met Ala Asp Gly Arg Ser Ser Ala Ser Val Val Ala Val Asp Pro Glu 
1               5                   10                  15      


Lys Ala Ala Arg Glu Arg Asp Glu Ala Ala Arg Ala Leu Leu Arg Asp 
            20                  25                  30          


Ser Pro Leu Gln Thr His Leu Gln Tyr Met Thr Asn Gly Leu Glu Leu 
        35                  40                  45              


Thr Val Pro Phe Thr Leu Lys Val Val Ala Glu Ala Val Ala Phe Ser 
    50                  55                  60                  


Arg Ala Lys Glu Val Ala Asp Glu Val Leu Arg Ser Ala Trp His Leu 
65                  70                  75                  80  


Ala Asp Thr Val Leu Asn Asn Phe Asn Pro Asn Ser Glu Ile Ser Met 
                85                  90                  95      


Ile Gly Arg Leu Pro Val Gly Gln Lys His Thr Met Ser Ala Thr Leu 
            100                 105                 110         


Lys Ser Val Ile Thr Cys Cys Gln His Val Phe Asn Ser Ser Arg Gly 
        115                 120                 125             


Val Phe Asp Pro Ala Thr Gly Pro Ile Ile Glu Ala Leu Arg Ala Lys 
    130                 135                 140                 


Val Ala Glu Lys Ala Ser Val Ser Asp Glu Gln Met Glu Lys Leu Phe 
145                 150                 155                 160 


Arg Val Cys Asn Phe Ser Ser Ser Phe Ile Val Asp Leu Glu Met Gly 
                165                 170                 175     


Thr Ile Ala Arg Lys His Glu Asp Ala Arg Phe Asp Leu Gly Gly Val 
            180                 185                 190         


Ser Lys Gly Tyr Ile Val Asp Tyr Val Val Glu Arg Leu Asn Ala Ala 
        195                 200                 205             


Gly Ile Val Asp Val Tyr Phe Glu Trp Gly Gly Asp Cys Arg Ala Ser 
    210                 215                 220                 


Gly Thr Asn Ala Arg Arg Thr Pro Trp Met Val Gly Ile Ile Arg Pro 
225                 230                 235                 240 


Pro Ser Leu Glu Gln Leu Arg Asn Pro Pro Lys Asp Pro Ser Tyr Ile 
                245                 250                 255     


Arg Val Leu Pro Leu Asn Asp Glu Ala Leu Cys Thr Ser Gly Asp Tyr 
            260                 265                 270         


Glu Asn Leu Thr Glu Gly Ser Asn Lys Lys Leu Tyr Thr Ser Ile Phe 
        275                 280                 285             


Asp Trp Lys Lys Arg Ser Leu Leu Glu Pro Val Glu Ser Glu Leu Ala 
    290                 295                 300                 


Gln Val Ser Ile Arg Cys Tyr Ser Ala Met Tyr Ala Asp Ala Leu Ala 
305                 310                 315                 320 


Thr Ala Ser Leu Ile Lys Arg Asp Ile Lys Lys Val Arg Gln Met Leu 
                325                 330                 335     


Glu Asp Trp Arg His Val Arg Asn Arg Val Thr Asn Tyr Val Thr Tyr 
            340                 345                 350         


Thr Arg Gln Gly Glu Arg Val Ala Arg Met Phe Glu Ile Ala Thr Asp 
        355                 360                 365             


Asn Ala Glu Ile Arg Lys Lys Arg Ile Ala Gly Ser Leu Pro Ala Arg 
    370                 375                 380                 


Val Ile Val Val Gly Gly Gly Leu Ala Gly Leu Ser Ala Ala Ile Glu 
385                 390                 395                 400 


Ala Thr Ala Cys Gly Ala Gln Val Ile Leu Leu Glu Lys Glu Pro Lys 
                405                 410                 415     


Val Gly Gly Asn Ser Ala Lys Ala Thr Ser Gly Ile Asn Gly Trp Gly 
            420                 425                 430         


Thr Arg Ala Gln Ala Glu Gln Asp Val Tyr Asp Ser Gly Lys Tyr Phe 
        435                 440                 445             


Glu Arg Asp Thr His Lys Ser Gly Leu Gly Gly Ser Thr Asp Pro Gly 
    450                 455                 460                 


Leu Val Arg Thr Leu Ser Val Lys Ser Gly Asp Ala Ile Ser Trp Leu 
465                 470                 475                 480 


Ser Ser Leu Gly Val Pro Leu Thr Val Leu Ser Gln Leu Gly Gly His 
                485                 490                 495     


Ser Arg Lys Arg Thr His Arg Ala Pro Asp Lys Ala Asp Gly Thr Pro 
            500                 505                 510         


Val Pro Ile Gly Phe Thr Ile Met Gln Thr Leu Glu Gln His Val Arg 
        515                 520                 525             


Thr Lys Leu Ala Asp Arg Val Thr Ile Met Glu Asn Thr Thr Val Thr 
    530                 535                 540                 


Ser Leu Leu Ser Lys Ser Arg Val Arg His Asp Gly Ala Lys Gln Val 
545                 550                 555                 560 


Arg Val Tyr Gly Val Glu Val Leu Gln Asp Glu Gly Val Val Ser Arg 
                565                 570                 575     


Ile Leu Ala Asp Ala Val Ile Leu Ala Thr Gly Gly Phe Ser Asn Asp 
            580                 585                 590         


Lys Thr Pro Asn Ser Leu Leu Gln Glu Phe Ala Pro Gln Leu Ser Gly 
        595                 600                 605             


Phe Pro Thr Thr Asn Gly Pro Trp Ala Thr Gly Asp Gly Val Lys Leu 
    610                 615                 620                 


Ala Arg Glu Leu Gly Val Lys Leu Val Asp Met Asp Lys Val Gln Leu 
625                 630                 635                 640 


His Pro Thr Gly Leu Ile Asp Pro Lys Asp Pro Ala Asn Pro Thr Lys 
                645                 650                 655     


Tyr Leu Gly Pro Glu Ala Leu Arg Gly Ser Gly Gly Val Leu Leu Asn 
            660                 665                 670         


Lys Lys Gly Glu Arg Phe Val Asn Glu Leu Asp Leu Arg Ser Val Val 
        675                 680                 685             


Ser Asn Ala Ile Ile Glu Gln Gly Asp Glu Tyr Pro Asp Ala Gly Gly 
    690                 695                 700                 


Ser Lys Phe Ala Phe Cys Val Leu Asn Asp Ala Ala Val Lys Leu Phe 
705                 710                 715                 720 


Gly Val Asn Ser His Gly Phe Tyr Trp Lys Arg Leu Gly Leu Phe Val 
                725                 730                 735     


Lys Ala Asp Thr Val Glu Lys Leu Ala Ala Leu Ile Gly Cys Pro Val 
            740                 745                 750         


Glu Asn Val Arg Asn Thr Leu Gly Asp Tyr Glu Gln Leu Ser Lys Glu 
        755                 760                 765             


Asn Arg Gln Cys Pro Lys Thr Arg Lys Val Val Tyr Pro Cys Val Val 
    770                 775                 780                 


Gly Pro Gln Gly Pro Phe Tyr Val Ala Phe Val Thr Pro Ser Ile His 
785                 790                 795                 800 


Tyr Thr Met Gly Gly Cys Leu Ile Ser Pro Ser Ala Glu Met Gln Leu 
                805                 810                 815     


Glu Glu Asn Thr Thr Ser Pro Phe Gly His Arg Arg Pro Ile Phe Gly 
            820                 825                 830         


Leu Phe Gly Ala Gly Glu Val Thr Gly Gly Val His Gly Gly Asn Arg 
        835                 840                 845             


Leu Gly Gly Asn Ser Leu Leu Glu Cys Val Val Phe Gly Arg Ile Ala 
    850                 855                 860                 


Gly Asp Arg Ala Ala Thr Ile Leu Gln Lys Lys Pro Val Pro Leu Ser 
865                 870                 875                 880 


Phe Lys Thr Trp Thr Thr Val Ile Leu Arg Glu Val Arg Glu Gly Gly 
                885                 890                 895     


Met Tyr Gly Thr Gly Ser Arg Val Leu Arg Phe Asn Leu Pro Gly Ala 
            900                 905                 910         


Leu Gln Arg Ser Gly Leu Gln Leu Gly Gln Phe Ile Ala Ile Arg Gly 
        915                 920                 925             


Glu Trp Asp Gly Gln Gln Leu Ile Gly Tyr Tyr Ser Pro Ile Thr Leu 
    930                 935                 940                 


Pro Asp Asp Leu Gly Val Ile Gly Ile Leu Ala Arg Ser Asp Lys Gly 
945                 950                 955                 960 


Thr Leu Lys Glu Trp Ile Ser Ala Leu Glu Pro Gly Asp Ala Val Glu 
                965                 970                 975     


Met Lys Gly Cys Gly Gly Leu Val Ile Glu Arg Arg Phe Ser Glu Arg 
            980                 985                 990         


Tyr Leu Tyr Phe Ser Gly His Ala  Leu Lys Lys Leu Cys  Leu Ile Ala 
        995                 1000                 1005             


Gly Gly  Thr Gly Val Ala Pro  Met Leu Gln Ile Ile  Arg Ala Ala 
    1010                 1015                 1020             


Leu Lys  Lys Pro Phe Leu Glu  Asn Ile Glu Ser Ile  Arg Leu Ile 
    1025                 1030                 1035             


Tyr Ala  Ala Glu Asp Val Ser  Glu Leu Thr Tyr Arg  Glu Leu Leu 
    1040                 1045                 1050             


Glu His  His Gln Arg Asp Ser  Lys Gly Lys Phe Arg  Ser Ile Phe 
    1055                 1060                 1065             


Val Leu  Asn Arg Pro Pro Pro  Ile Trp Thr Asp Gly  Val Gly Phe 
    1070                 1075                 1080             


Ile Asp  Lys Lys Leu Leu Ser  Ser Ser Val Gln Pro  Pro Ala Lys 
    1085                 1090                 1095             


Asp Leu  Leu Val Ala Ile Cys  Gly Pro Pro Ile Met  Gln Arg Val 
    1100                 1105                 1110             


Val Lys  Thr Cys Leu Lys Ser  Leu Gly Tyr Asp Met  Gln Leu Val 
    1115                 1120                 1125             


Arg Thr  Val Asp Glu Val Glu  Thr Gln Asn Ser 
    1130                 1135                 


<210>  41
<211>  3435
<212>  DNA
<213>  Leishmania braziliensis


<220>
<221>  CDS
<222>  (1)..(3435)

<400>  41
atg gct gat ggt aaa acc tct gct tcc gtt gtt gct gtc gac cca gag         48
Met Ala Asp Gly Lys Thr Ser Ala Ser Val Val Ala Val Asp Pro Glu           
1               5                   10                  15                

cgt gca gca aag gag aga gat gca gca gca aga gca atg tta caa gac         96
Arg Ala Ala Lys Glu Arg Asp Ala Ala Ala Arg Ala Met Leu Gln Asp           
            20                  25                  30                    

ggt ggt gtt tct cca gtt ggt aaa gct cag ttg ttg aaa aag ggt ttg        144
Gly Gly Val Ser Pro Val Gly Lys Ala Gln Leu Leu Lys Lys Gly Leu           
        35                  40                  45                        

gca tat gct gtc cct tac acc ctt aag att gtt gtt gca gat cct aaa        192
Ala Tyr Ala Val Pro Tyr Thr Leu Lys Ile Val Val Ala Asp Pro Lys           
    50                  55                  60                            

gct atg gaa aag acc acc gca gac gtt gag aag gtc ctt caa acc gca        240
Ala Met Glu Lys Thr Thr Ala Asp Val Glu Lys Val Leu Gln Thr Ala           
65                  70                  75                  80            

ttc caa gtc gtt gac act ttg tta aac aat ttc aac gaa aac tcc gag        288
Phe Gln Val Val Asp Thr Leu Leu Asn Asn Phe Asn Glu Asn Ser Glu           
                85                  90                  95                

gtt tct cgt atc aac aga atg cca gtc ggt gag gaa cac caa atg tct        336
Val Ser Arg Ile Asn Arg Met Pro Val Gly Glu Glu His Gln Met Ser           
            100                 105                 110                   

gct gca ttg aag aga gtt atg ggt tgc tgt cag cgt gtt tac aat tca        384
Ala Ala Leu Lys Arg Val Met Gly Cys Cys Gln Arg Val Tyr Asn Ser           
        115                 120                 125                       

tct cgt ggt gct ttt gac cca gct gtt ggt cca ttg gtc aga gaa ttg        432
Ser Arg Gly Ala Phe Asp Pro Ala Val Gly Pro Leu Val Arg Glu Leu           
    130                 135                 140                           

agg gaa gct gca aga gaa ggc aga act tta cca gca gaa agg att aac        480
Arg Glu Ala Ala Arg Glu Gly Arg Thr Leu Pro Ala Glu Arg Ile Asn           
145                 150                 155                 160           

gct ttg tta tcc aag tgt acc ttg aat atc tcc ttt tcc att gat ttg        528
Ala Leu Leu Ser Lys Cys Thr Leu Asn Ile Ser Phe Ser Ile Asp Leu           
                165                 170                 175               

aac aga ggt act att gcc aga aaa cac gca gat gca atg ttg gat ttg        576
Asn Arg Gly Thr Ile Ala Arg Lys His Ala Asp Ala Met Leu Asp Leu           
            180                 185                 190                   

ggt ggt gtc aat aag ggt tat ggt gtt gat tat gtt gtc gaa cat ttg        624
Gly Gly Val Asn Lys Gly Tyr Gly Val Asp Tyr Val Val Glu His Leu           
        195                 200                 205                       

aac aat ttg ggt tat gat gat gtc ttt ttc gaa tgg ggt ggt gat gtt        672
Asn Asn Leu Gly Tyr Asp Asp Val Phe Phe Glu Trp Gly Gly Asp Val           
    210                 215                 220                           

aga gca tct ggc aaa aac cca tca aac caa cat tgg gtt gtt ggt att        720
Arg Ala Ser Gly Lys Asn Pro Ser Asn Gln His Trp Val Val Gly Ile           
225                 230                 235                 240           

gct aga cca cca gca ctt gct gat atc aga acc gtt gtt cca caa gac        768
Ala Arg Pro Pro Ala Leu Ala Asp Ile Arg Thr Val Val Pro Gln Asp           
                245                 250                 255               

aag caa tcc ttc atc aga gtt gtt tgt ctt aat gat gaa gca att gcc        816
Lys Gln Ser Phe Ile Arg Val Val Cys Leu Asn Asp Glu Ala Ile Ala           
            260                 265                 270                   

acc tct ggt gat tac gaa aat ctt gtc gaa ggt cct ggt tct aag gtt        864
Thr Ser Gly Asp Tyr Glu Asn Leu Val Glu Gly Pro Gly Ser Lys Val           
        275                 280                 285                       

tac tcc tct acc ttc aac gca acc tct aag tcc tta ttg gaa cca acc        912
Tyr Ser Ser Thr Phe Asn Ala Thr Ser Lys Ser Leu Leu Glu Pro Thr           
    290                 295                 300                           

gaa acc aat atc gca caa gtc tct gtt aag tgt tac tca tgc atg tat        960
Glu Thr Asn Ile Ala Gln Val Ser Val Lys Cys Tyr Ser Cys Met Tyr           
305                 310                 315                 320           

gca gac gca ttg gct acc gct gcc tta ttg aaa aac aat cca act gct       1008
Ala Asp Ala Leu Ala Thr Ala Ala Leu Leu Lys Asn Asn Pro Thr Ala           
                325                 330                 335               

gtt cgt aga atg tta gat aac tgg aga tat gtt cgt gat act gtt acc       1056
Val Arg Arg Met Leu Asp Asn Trp Arg Tyr Val Arg Asp Thr Val Thr           
            340                 345                 350                   

gac tat aca acc tat tcc aga gaa ggt gaa aga gtt gca aag atg ttt       1104
Asp Tyr Thr Thr Tyr Ser Arg Glu Gly Glu Arg Val Ala Lys Met Phe           
        355                 360                 365                       

gag att gca acc gaa gat aag gaa atg aga gct aag aga att tcc ggt       1152
Glu Ile Ala Thr Glu Asp Lys Glu Met Arg Ala Lys Arg Ile Ser Gly           
    370                 375                 380                           

tcc ttg cca gca aga gtc att atc gtc ggt ggt ggt tta gct ggt tgt       1200
Ser Leu Pro Ala Arg Val Ile Ile Val Gly Gly Gly Leu Ala Gly Cys           
385                 390                 395                 400           

tct gca gct att gaa gca gtc aac tgt ggt gct caa gtc att ttg tta       1248
Ser Ala Ala Ile Glu Ala Val Asn Cys Gly Ala Gln Val Ile Leu Leu           
                405                 410                 415               

gaa aag gaa gcc aag att ggt ggc aac tcc gca aag gct acc tct ggt       1296
Glu Lys Glu Ala Lys Ile Gly Gly Asn Ser Ala Lys Ala Thr Ser Gly           
            420                 425                 430                   

atc aac gcc tgg ggt act aga gcc cag gct aaa caa ggt gtt atg gat       1344
Ile Asn Ala Trp Gly Thr Arg Ala Gln Ala Lys Gln Gly Val Met Asp           
        435                 440                 445                       

ggt ggc aag ttt ttc gag aga gac acc cat aga tcc ggt aaa ggt ggt       1392
Gly Gly Lys Phe Phe Glu Arg Asp Thr His Arg Ser Gly Lys Gly Gly           
    450                 455                 460                           

cac tgt gat cct tgt ttg gtt aag aca ctt tcc gtt aag tca tca gac       1440
His Cys Asp Pro Cys Leu Val Lys Thr Leu Ser Val Lys Ser Ser Asp           
465                 470                 475                 480           

gca gtt aag tgg ttg tct gaa ttg ggt gtt cca tta acc gtc tta tcc       1488
Ala Val Lys Trp Leu Ser Glu Leu Gly Val Pro Leu Thr Val Leu Ser           
                485                 490                 495               

caa tta ggt ggt gca tcc aga aag agg tgt cat aga gcc cca gat aag       1536
Gln Leu Gly Gly Ala Ser Arg Lys Arg Cys His Arg Ala Pro Asp Lys           
            500                 505                 510                   

tct gat ggt act cct gtt cca att ggt ttt aca atc atg aaa aca tta       1584
Ser Asp Gly Thr Pro Val Pro Ile Gly Phe Thr Ile Met Lys Thr Leu           
        515                 520                 525                       

gaa aat cac atc att aac gat ctt tct cac caa gtt act gtt atg act       1632
Glu Asn His Ile Ile Asn Asp Leu Ser His Gln Val Thr Val Met Thr           
    530                 535                 540                           

ggt atc aag gtt act ggt ttg gag tcc act tct cac gct cgt cca gat       1680
Gly Ile Lys Val Thr Gly Leu Glu Ser Thr Ser His Ala Arg Pro Asp           
545                 550                 555                 560           

ggt gtt tta gtt aag cac gtt act ggt gtt aga ttg att caa ggt gat       1728
Gly Val Leu Val Lys His Val Thr Gly Val Arg Leu Ile Gln Gly Asp           
                565                 570                 575               

ggc caa tcc aga gtt ttg aat gct gat gcc gtt atc tta gca act ggt       1776
Gly Gln Ser Arg Val Leu Asn Ala Asp Ala Val Ile Leu Ala Thr Gly           
            580                 585                 590                   

ggt ttc tcc aat gac cat act gct aac tct tta ctt caa caa tac gct       1824
Gly Phe Ser Asn Asp His Thr Ala Asn Ser Leu Leu Gln Gln Tyr Ala           
        595                 600                 605                       

cca caa ctt tca tcc ttt cca acc act aat ggt gtt tgg gcc act ggt       1872
Pro Gln Leu Ser Ser Phe Pro Thr Thr Asn Gly Val Trp Ala Thr Gly           
    610                 615                 620                           

gac ggt gtc aag gca gct aga gaa tta ggt gtt gag ttg gtt gac atg       1920
Asp Gly Val Lys Ala Ala Arg Glu Leu Gly Val Glu Leu Val Asp Met           
625                 630                 635                 640           

gat aag gtc caa ttg cat cca aca ggt ttg tta gat cca aag gac cca       1968
Asp Lys Val Gln Leu His Pro Thr Gly Leu Leu Asp Pro Lys Asp Pro           
                645                 650                 655               

tcc aac agg act aag tac ttg ggt cca gaa gct tta agg ggt tca ggc       2016
Ser Asn Arg Thr Lys Tyr Leu Gly Pro Glu Ala Leu Arg Gly Ser Gly           
            660                 665                 670                   

ggt gtc ttg tta aac aaa aac ggt gaa cgt ttc gtc aac gaa ctt gat       2064
Gly Val Leu Leu Asn Lys Asn Gly Glu Arg Phe Val Asn Glu Leu Asp           
        675                 680                 685                       

ttg aga tct gtc gtt tct caa gcc att atc gaa caa aac aac gtt tac       2112
Leu Arg Ser Val Val Ser Gln Ala Ile Ile Glu Gln Asn Asn Val Tyr           
    690                 695                 700                           

cct ggt tct ggt ggt tcc aag ttt gct tac tgc gtt ttg aac gaa gca       2160
Pro Gly Ser Gly Gly Ser Lys Phe Ala Tyr Cys Val Leu Asn Glu Ala           
705                 710                 715                 720           

gca gct aag ttg ttc ggc aaa aac ttt ttg ggt ttc tat tgg cat aga       2208
Ala Ala Lys Leu Phe Gly Lys Asn Phe Leu Gly Phe Tyr Trp His Arg           
                725                 730                 735               

tta ggt ctt ttt gaa aag gtt gaa gat gtt gct ggt tta gcc aaa ttg       2256
Leu Gly Leu Phe Glu Lys Val Glu Asp Val Ala Gly Leu Ala Lys Leu           
            740                 745                 750                   

atc ggt tgt cca gag gaa aat gtt acc gct aca ttg aag gaa tac aag       2304
Ile Gly Cys Pro Glu Glu Asn Val Thr Ala Thr Leu Lys Glu Tyr Lys           
        755                 760                 765                       

gaa ttg tcc tcc aaa aag ctt cat gcc tgt cct tta acc aac aaa aac       2352
Glu Leu Ser Ser Lys Lys Leu His Ala Cys Pro Leu Thr Asn Lys Asn           
    770                 775                 780                           

gtc ttt cct tgc act tta ggt act gaa ggc cct tac tat gtt gct ttc       2400
Val Phe Pro Cys Thr Leu Gly Thr Glu Gly Pro Tyr Tyr Val Ala Phe           
785                 790                 795                 800           

gtc aca cct tca att cac tac aca atg ggt ggt tgt ttg atc tcc cct       2448
Val Thr Pro Ser Ile His Tyr Thr Met Gly Gly Cys Leu Ile Ser Pro           
                805                 810                 815               

tca gca gaa atg cag acc att gat aac act ggt gtc aca cca gtt cgt       2496
Ser Ala Glu Met Gln Thr Ile Asp Asn Thr Gly Val Thr Pro Val Arg           
            820                 825                 830                   

aga cca atc ttg ggc tta ttc ggt gct ggt gaa gtt act ggt ggt gtc       2544
Arg Pro Ile Leu Gly Leu Phe Gly Ala Gly Glu Val Thr Gly Gly Val           
        835                 840                 845                       

cat ggt ggt aac aga ttg ggt ggt aat tcc tta ttg gaa tgt gtt gtc       2592
His Gly Gly Asn Arg Leu Gly Gly Asn Ser Leu Leu Glu Cys Val Val           
    850                 855                 860                           

ttt ggt aga att gct ggt gat aga gcc gct acc att ttg caa aag aag       2640
Phe Gly Arg Ile Ala Gly Asp Arg Ala Ala Thr Ile Leu Gln Lys Lys           
865                 870                 875                 880           

aat gct ggt tta tca atg act gag tgg tct aca gtt gtc tta aga gaa       2688
Asn Ala Gly Leu Ser Met Thr Glu Trp Ser Thr Val Val Leu Arg Glu           
                885                 890                 895               

gtc aga gaa ggc ggt gtt tac ggt act ggt tct cgt gtc ctt aga ttc       2736
Val Arg Glu Gly Gly Val Tyr Gly Thr Gly Ser Arg Val Leu Arg Phe           
            900                 905                 910                   

aat atg cca ggt gcc tta caa aag act ggc tta gca ttg ggt caa ttc       2784
Asn Met Pro Gly Ala Leu Gln Lys Thr Gly Leu Ala Leu Gly Gln Phe           
        915                 920                 925                       

atc gca atg aga ggt gat tgg gat ggt caa cag tta ttg ggt tac tat       2832
Ile Ala Met Arg Gly Asp Trp Asp Gly Gln Gln Leu Leu Gly Tyr Tyr           
    930                 935                 940                           

tct cca att aca tta cca gac gac att ggt gtt att ggt atc tta gct       2880
Ser Pro Ile Thr Leu Pro Asp Asp Ile Gly Val Ile Gly Ile Leu Ala           
945                 950                 955                 960           

aga gct gac aaa ggt aga tta gct gaa tgg att tct gca tta caa cca       2928
Arg Ala Asp Lys Gly Arg Leu Ala Glu Trp Ile Ser Ala Leu Gln Pro           
                965                 970                 975               

ggt gat gct gtt gag atg aag gca tgt ggc ggt ttg att atc cat aga       2976
Gly Asp Ala Val Glu Met Lys Ala Cys Gly Gly Leu Ile Ile His Arg           
            980                 985                 990                   

aga ttc gct gct aga cac ttg ttt  ttc cgt tct cac aag  att aga aag     3024
Arg Phe Ala Ala Arg His Leu Phe  Phe Arg Ser His Lys  Ile Arg Lys         
        995                 1000                 1005                     

ctt gct  ctt att ggt ggt ggt  act ggt gtt gca cca  atg ttg caa        3069
Leu Ala  Leu Ile Gly Gly Gly  Thr Gly Val Ala Pro  Met Leu Gln            
    1010                 1015                 1020                        

att gtc  agg gct gca gtc aaa  aag cca ttt gtt gac  tct att gag        3114
Ile Val  Arg Ala Ala Val Lys  Lys Pro Phe Val Asp  Ser Ile Glu            
    1025                 1030                 1035                        

tct att  cag ttc atc tat gca  gct gaa gat gtc tcc  gaa ctt act        3159
Ser Ile  Gln Phe Ile Tyr Ala  Ala Glu Asp Val Ser  Glu Leu Thr            
    1040                 1045                 1050                        

tat aga  act ttg ttg gaa tca  tat gaa aag gaa tac  ggt tct ggc        3204
Tyr Arg  Thr Leu Leu Glu Ser  Tyr Glu Lys Glu Tyr  Gly Ser Gly            
    1055                 1060                 1065                        

aaa ttc  aag tgt cat ttc gtc  ttg aat aac cca cca  tca caa tgg        3249
Lys Phe  Lys Cys His Phe Val  Leu Asn Asn Pro Pro  Ser Gln Trp            
    1070                 1075                 1080                        

acc gag  ggc gtt ggt ttc gtt  gat act gct ttg ttg  cgt tct gcc        3294
Thr Glu  Gly Val Gly Phe Val  Asp Thr Ala Leu Leu  Arg Ser Ala            
    1085                 1090                 1095                        

gtt caa  gca cct tct aac gac  ttg tta gtc gct att  tgt ggc cca        3339
Val Gln  Ala Pro Ser Asn Asp  Leu Leu Val Ala Ile  Cys Gly Pro            
    1100                 1105                 1110                        

cca atc  atg caa aga gca gtc  aaa tca gcc tta aag  ggt tta ggt        3384
Pro Ile  Met Gln Arg Ala Val  Lys Ser Ala Leu Lys  Gly Leu Gly            
    1115                 1120                 1125                        

tac aat  atg aat ttg gtt aga  aca gtt gat gaa cca  gaa cca ttg        3429
Tyr Asn  Met Asn Leu Val Arg  Thr Val Asp Glu Pro  Glu Pro Leu            
    1130                 1135                 1140                        

tct taa                                                               3435
Ser                                                                       
                                                                          


<210>  42
<211>  1144
<212>  PRT
<213>  Leishmania braziliensis

<400>  42

Met Ala Asp Gly Lys Thr Ser Ala Ser Val Val Ala Val Asp Pro Glu 
1               5                   10                  15      


Arg Ala Ala Lys Glu Arg Asp Ala Ala Ala Arg Ala Met Leu Gln Asp 
            20                  25                  30          


Gly Gly Val Ser Pro Val Gly Lys Ala Gln Leu Leu Lys Lys Gly Leu 
        35                  40                  45              


Ala Tyr Ala Val Pro Tyr Thr Leu Lys Ile Val Val Ala Asp Pro Lys 
    50                  55                  60                  


Ala Met Glu Lys Thr Thr Ala Asp Val Glu Lys Val Leu Gln Thr Ala 
65                  70                  75                  80  


Phe Gln Val Val Asp Thr Leu Leu Asn Asn Phe Asn Glu Asn Ser Glu 
                85                  90                  95      


Val Ser Arg Ile Asn Arg Met Pro Val Gly Glu Glu His Gln Met Ser 
            100                 105                 110         


Ala Ala Leu Lys Arg Val Met Gly Cys Cys Gln Arg Val Tyr Asn Ser 
        115                 120                 125             


Ser Arg Gly Ala Phe Asp Pro Ala Val Gly Pro Leu Val Arg Glu Leu 
    130                 135                 140                 


Arg Glu Ala Ala Arg Glu Gly Arg Thr Leu Pro Ala Glu Arg Ile Asn 
145                 150                 155                 160 


Ala Leu Leu Ser Lys Cys Thr Leu Asn Ile Ser Phe Ser Ile Asp Leu 
                165                 170                 175     


Asn Arg Gly Thr Ile Ala Arg Lys His Ala Asp Ala Met Leu Asp Leu 
            180                 185                 190         


Gly Gly Val Asn Lys Gly Tyr Gly Val Asp Tyr Val Val Glu His Leu 
        195                 200                 205             


Asn Asn Leu Gly Tyr Asp Asp Val Phe Phe Glu Trp Gly Gly Asp Val 
    210                 215                 220                 


Arg Ala Ser Gly Lys Asn Pro Ser Asn Gln His Trp Val Val Gly Ile 
225                 230                 235                 240 


Ala Arg Pro Pro Ala Leu Ala Asp Ile Arg Thr Val Val Pro Gln Asp 
                245                 250                 255     


Lys Gln Ser Phe Ile Arg Val Val Cys Leu Asn Asp Glu Ala Ile Ala 
            260                 265                 270         


Thr Ser Gly Asp Tyr Glu Asn Leu Val Glu Gly Pro Gly Ser Lys Val 
        275                 280                 285             


Tyr Ser Ser Thr Phe Asn Ala Thr Ser Lys Ser Leu Leu Glu Pro Thr 
    290                 295                 300                 


Glu Thr Asn Ile Ala Gln Val Ser Val Lys Cys Tyr Ser Cys Met Tyr 
305                 310                 315                 320 


Ala Asp Ala Leu Ala Thr Ala Ala Leu Leu Lys Asn Asn Pro Thr Ala 
                325                 330                 335     


Val Arg Arg Met Leu Asp Asn Trp Arg Tyr Val Arg Asp Thr Val Thr 
            340                 345                 350         


Asp Tyr Thr Thr Tyr Ser Arg Glu Gly Glu Arg Val Ala Lys Met Phe 
        355                 360                 365             


Glu Ile Ala Thr Glu Asp Lys Glu Met Arg Ala Lys Arg Ile Ser Gly 
    370                 375                 380                 


Ser Leu Pro Ala Arg Val Ile Ile Val Gly Gly Gly Leu Ala Gly Cys 
385                 390                 395                 400 


Ser Ala Ala Ile Glu Ala Val Asn Cys Gly Ala Gln Val Ile Leu Leu 
                405                 410                 415     


Glu Lys Glu Ala Lys Ile Gly Gly Asn Ser Ala Lys Ala Thr Ser Gly 
            420                 425                 430         


Ile Asn Ala Trp Gly Thr Arg Ala Gln Ala Lys Gln Gly Val Met Asp 
        435                 440                 445             


Gly Gly Lys Phe Phe Glu Arg Asp Thr His Arg Ser Gly Lys Gly Gly 
    450                 455                 460                 


His Cys Asp Pro Cys Leu Val Lys Thr Leu Ser Val Lys Ser Ser Asp 
465                 470                 475                 480 


Ala Val Lys Trp Leu Ser Glu Leu Gly Val Pro Leu Thr Val Leu Ser 
                485                 490                 495     


Gln Leu Gly Gly Ala Ser Arg Lys Arg Cys His Arg Ala Pro Asp Lys 
            500                 505                 510         


Ser Asp Gly Thr Pro Val Pro Ile Gly Phe Thr Ile Met Lys Thr Leu 
        515                 520                 525             


Glu Asn His Ile Ile Asn Asp Leu Ser His Gln Val Thr Val Met Thr 
    530                 535                 540                 


Gly Ile Lys Val Thr Gly Leu Glu Ser Thr Ser His Ala Arg Pro Asp 
545                 550                 555                 560 


Gly Val Leu Val Lys His Val Thr Gly Val Arg Leu Ile Gln Gly Asp 
                565                 570                 575     


Gly Gln Ser Arg Val Leu Asn Ala Asp Ala Val Ile Leu Ala Thr Gly 
            580                 585                 590         


Gly Phe Ser Asn Asp His Thr Ala Asn Ser Leu Leu Gln Gln Tyr Ala 
        595                 600                 605             


Pro Gln Leu Ser Ser Phe Pro Thr Thr Asn Gly Val Trp Ala Thr Gly 
    610                 615                 620                 


Asp Gly Val Lys Ala Ala Arg Glu Leu Gly Val Glu Leu Val Asp Met 
625                 630                 635                 640 


Asp Lys Val Gln Leu His Pro Thr Gly Leu Leu Asp Pro Lys Asp Pro 
                645                 650                 655     


Ser Asn Arg Thr Lys Tyr Leu Gly Pro Glu Ala Leu Arg Gly Ser Gly 
            660                 665                 670         


Gly Val Leu Leu Asn Lys Asn Gly Glu Arg Phe Val Asn Glu Leu Asp 
        675                 680                 685             


Leu Arg Ser Val Val Ser Gln Ala Ile Ile Glu Gln Asn Asn Val Tyr 
    690                 695                 700                 


Pro Gly Ser Gly Gly Ser Lys Phe Ala Tyr Cys Val Leu Asn Glu Ala 
705                 710                 715                 720 


Ala Ala Lys Leu Phe Gly Lys Asn Phe Leu Gly Phe Tyr Trp His Arg 
                725                 730                 735     


Leu Gly Leu Phe Glu Lys Val Glu Asp Val Ala Gly Leu Ala Lys Leu 
            740                 745                 750         


Ile Gly Cys Pro Glu Glu Asn Val Thr Ala Thr Leu Lys Glu Tyr Lys 
        755                 760                 765             


Glu Leu Ser Ser Lys Lys Leu His Ala Cys Pro Leu Thr Asn Lys Asn 
    770                 775                 780                 


Val Phe Pro Cys Thr Leu Gly Thr Glu Gly Pro Tyr Tyr Val Ala Phe 
785                 790                 795                 800 


Val Thr Pro Ser Ile His Tyr Thr Met Gly Gly Cys Leu Ile Ser Pro 
                805                 810                 815     


Ser Ala Glu Met Gln Thr Ile Asp Asn Thr Gly Val Thr Pro Val Arg 
            820                 825                 830         


Arg Pro Ile Leu Gly Leu Phe Gly Ala Gly Glu Val Thr Gly Gly Val 
        835                 840                 845             


His Gly Gly Asn Arg Leu Gly Gly Asn Ser Leu Leu Glu Cys Val Val 
    850                 855                 860                 


Phe Gly Arg Ile Ala Gly Asp Arg Ala Ala Thr Ile Leu Gln Lys Lys 
865                 870                 875                 880 


Asn Ala Gly Leu Ser Met Thr Glu Trp Ser Thr Val Val Leu Arg Glu 
                885                 890                 895     


Val Arg Glu Gly Gly Val Tyr Gly Thr Gly Ser Arg Val Leu Arg Phe 
            900                 905                 910         


Asn Met Pro Gly Ala Leu Gln Lys Thr Gly Leu Ala Leu Gly Gln Phe 
        915                 920                 925             


Ile Ala Met Arg Gly Asp Trp Asp Gly Gln Gln Leu Leu Gly Tyr Tyr 
    930                 935                 940                 


Ser Pro Ile Thr Leu Pro Asp Asp Ile Gly Val Ile Gly Ile Leu Ala 
945                 950                 955                 960 


Arg Ala Asp Lys Gly Arg Leu Ala Glu Trp Ile Ser Ala Leu Gln Pro 
                965                 970                 975     


Gly Asp Ala Val Glu Met Lys Ala Cys Gly Gly Leu Ile Ile His Arg 
            980                 985                 990         


Arg Phe Ala Ala Arg His Leu Phe  Phe Arg Ser His Lys  Ile Arg Lys 
        995                 1000                 1005             


Leu Ala  Leu Ile Gly Gly Gly  Thr Gly Val Ala Pro  Met Leu Gln 
    1010                 1015                 1020             


Ile Val  Arg Ala Ala Val Lys  Lys Pro Phe Val Asp  Ser Ile Glu 
    1025                 1030                 1035             


Ser Ile  Gln Phe Ile Tyr Ala  Ala Glu Asp Val Ser  Glu Leu Thr 
    1040                 1045                 1050             


Tyr Arg  Thr Leu Leu Glu Ser  Tyr Glu Lys Glu Tyr  Gly Ser Gly 
    1055                 1060                 1065             


Lys Phe  Lys Cys His Phe Val  Leu Asn Asn Pro Pro  Ser Gln Trp 
    1070                 1075                 1080             


Thr Glu  Gly Val Gly Phe Val  Asp Thr Ala Leu Leu  Arg Ser Ala 
    1085                 1090                 1095             


Val Gln  Ala Pro Ser Asn Asp  Leu Leu Val Ala Ile  Cys Gly Pro 
    1100                 1105                 1110             


Pro Ile  Met Gln Arg Ala Val  Lys Ser Ala Leu Lys  Gly Leu Gly 
    1115                 1120                 1125             


Tyr Asn  Met Asn Leu Val Arg  Thr Val Asp Glu Pro  Glu Pro Leu 
    1130                 1135                 1140             


Ser 
    


<210>  43
<211>  3435
<212>  DNA
<213>  Leishmania mexicana


<220>
<221>  CDS
<222>  (1)..(3435)

<400>  43
atg gct gat ggc aaa acc tct gca tca gtt gtt gct gtt gat gct gaa         48
Met Ala Asp Gly Lys Thr Ser Ala Ser Val Val Ala Val Asp Ala Glu           
1               5                   10                  15                

cgt gcc gct aag gaa aga gat gca gca gct aga gct atg ttg caa ggt         96
Arg Ala Ala Lys Glu Arg Asp Ala Ala Ala Arg Ala Met Leu Gln Gly           
            20                  25                  30                    

ggt ggt gtc tct cct gct ggc aag gca caa ttg ttg aaa aag ggt ttg        144
Gly Gly Val Ser Pro Ala Gly Lys Ala Gln Leu Leu Lys Lys Gly Leu           
        35                  40                  45                        

gtt cac act gtt cca tat acc tta aag gtt gtc gtc gca gat cca aag        192
Val His Thr Val Pro Tyr Thr Leu Lys Val Val Val Ala Asp Pro Lys           
    50                  55                  60                            

gaa atg gag aag gca act gct gac gca gaa gag gtt tta caa gct gca        240
Glu Met Glu Lys Ala Thr Ala Asp Ala Glu Glu Val Leu Gln Ala Ala           
65                  70                  75                  80            

ttt caa gtc gtc gac acc ctt ttg aac aac ttt aac gaa aac tca gaa        288
Phe Gln Val Val Asp Thr Leu Leu Asn Asn Phe Asn Glu Asn Ser Glu           
                85                  90                  95                

gtt tca aga gtc aat agg ttg gca gtt ggt gag gaa cat caa atg tct        336
Val Ser Arg Val Asn Arg Leu Ala Val Gly Glu Glu His Gln Met Ser           
            100                 105                 110                   

gaa aca ttg aaa cac gtc atg gcc tgt tgt caa aag gtt tat cat tcc        384
Glu Thr Leu Lys His Val Met Ala Cys Cys Gln Lys Val Tyr His Ser           
        115                 120                 125                       

tcc aga ggt gtt ttt gac cca gca gtt ggt cca tta gtc cgt gaa ctt        432
Ser Arg Gly Val Phe Asp Pro Ala Val Gly Pro Leu Val Arg Glu Leu           
    130                 135                 140                           

aga gaa gct gct cac aag ggt aaa act gtt cca gcc gaa aga gtt aat        480
Arg Glu Ala Ala His Lys Gly Lys Thr Val Pro Ala Glu Arg Val Asn           
145                 150                 155                 160           

gat ttg tta tcc aaa tgt acc ctt aat gca tct ttt tca att gat atg        528
Asp Leu Leu Ser Lys Cys Thr Leu Asn Ala Ser Phe Ser Ile Asp Met           
                165                 170                 175               

tcc aga ggt atg att gca agg aag cat cca gac gcc atg ttg gat ttg        576
Ser Arg Gly Met Ile Ala Arg Lys His Pro Asp Ala Met Leu Asp Leu           
            180                 185                 190                   

ggt ggt gtc aac aag ggt tat ggt atc gac tac att gtt gaa cac tta        624
Gly Gly Val Asn Lys Gly Tyr Gly Ile Asp Tyr Ile Val Glu His Leu           
        195                 200                 205                       

aac tct ttg ggt tat gat gat gtc ttt ttc gaa tgg ggt ggt gat gtt        672
Asn Ser Leu Gly Tyr Asp Asp Val Phe Phe Glu Trp Gly Gly Asp Val           
    210                 215                 220                           

aga gca tcc ggc aaa aac cag tta tct caa cct tgg gct gtt ggt att        720
Arg Ala Ser Gly Lys Asn Gln Leu Ser Gln Pro Trp Ala Val Gly Ile           
225                 230                 235                 240           

gtt aga cca cct gcc ttg gcc gac att aga act gtt gtc cca gag gac        768
Val Arg Pro Pro Ala Leu Ala Asp Ile Arg Thr Val Val Pro Glu Asp           
                245                 250                 255               

aaa aga tcc ttt atc cgt gtc gtc aga ttg aac aac gaa gct att gct        816
Lys Arg Ser Phe Ile Arg Val Val Arg Leu Asn Asn Glu Ala Ile Ala           
            260                 265                 270                   

acc tct ggt gat tat gag aat ttg gtt gaa ggt cct ggt tct aag gtt        864
Thr Ser Gly Asp Tyr Glu Asn Leu Val Glu Gly Pro Gly Ser Lys Val           
        275                 280                 285                       

tac tct tcc acc ttc aat cca act tcc aaa aac ttg ttg gaa cct acc        912
Tyr Ser Ser Thr Phe Asn Pro Thr Ser Lys Asn Leu Leu Glu Pro Thr           
    290                 295                 300                           

gaa gca ggt atg gct caa gtt tct gtc aag tgt tgc tca tgt atc tac        960
Glu Ala Gly Met Ala Gln Val Ser Val Lys Cys Cys Ser Cys Ile Tyr           
305                 310                 315                 320           

gct gat gct tta gca aca gca gct ttg ttg aaa aac gat cct gct gcc       1008
Ala Asp Ala Leu Ala Thr Ala Ala Leu Leu Lys Asn Asp Pro Ala Ala           
                325                 330                 335               

gtt aga agg atc tta gat aac tgg aga tat gtc aga gat act gtt act       1056
Val Arg Arg Ile Leu Asp Asn Trp Arg Tyr Val Arg Asp Thr Val Thr           
            340                 345                 350                   

gac tac acc act tac aca agg gaa ggt gaa aga gtt gct aag atg ttg       1104
Asp Tyr Thr Thr Tyr Thr Arg Glu Gly Glu Arg Val Ala Lys Met Leu           
        355                 360                 365                       

gaa att gct acc gaa gat gct gaa atg aga gca aag aga atc aag ggc       1152
Glu Ile Ala Thr Glu Asp Ala Glu Met Arg Ala Lys Arg Ile Lys Gly           
    370                 375                 380                           

tct tta cca gca aga gtt atc att gtt ggt ggt ggt ttg gcc ggt tgt       1200
Ser Leu Pro Ala Arg Val Ile Ile Val Gly Gly Gly Leu Ala Gly Cys           
385                 390                 395                 400           

tcc gca gct atc gaa gca gct aac tgt ggc gcc cac gtc atc ttg tta       1248
Ser Ala Ala Ile Glu Ala Ala Asn Cys Gly Ala His Val Ile Leu Leu           
                405                 410                 415               

gaa aag gaa cca aag tta ggt ggt aac tct gca aag gct acc tcc ggt       1296
Glu Lys Glu Pro Lys Leu Gly Gly Asn Ser Ala Lys Ala Thr Ser Gly           
            420                 425                 430                   

atc aac gcc tgg ggt act aga gca caa gca aaa caa ggt gtc atg gac       1344
Ile Asn Ala Trp Gly Thr Arg Ala Gln Ala Lys Gln Gly Val Met Asp           
        435                 440                 445                       

ggc ggc aag ttt ttc gaa aga gat acc cat aga tcc ggc aag ggt ggt       1392
Gly Gly Lys Phe Phe Glu Arg Asp Thr His Arg Ser Gly Lys Gly Gly           
    450                 455                 460                           

aat tgc gat cca tgc ctt gtt aag act ttg tcc gtt aag tcc tct gat       1440
Asn Cys Asp Pro Cys Leu Val Lys Thr Leu Ser Val Lys Ser Ser Asp           
465                 470                 475                 480           

gca gtt aag tgg tta tct gaa tta ggt gtt cca ttg act gtt ttg tct       1488
Ala Val Lys Trp Leu Ser Glu Leu Gly Val Pro Leu Thr Val Leu Ser           
                485                 490                 495               

caa tta ggt ggt gct tca agg aaa cgt tgt cac cgt gca cca gat aag       1536
Gln Leu Gly Gly Ala Ser Arg Lys Arg Cys His Arg Ala Pro Asp Lys           
            500                 505                 510                   

tct gat ggt aca cca gtc cca gtt ggt ttc acc att atg aaa acc ctt       1584
Ser Asp Gly Thr Pro Val Pro Val Gly Phe Thr Ile Met Lys Thr Leu           
        515                 520                 525                       

gaa aac cac att gtc aac gat ttg tcc aga cat gtt aca gtt atg aca       1632
Glu Asn His Ile Val Asn Asp Leu Ser Arg His Val Thr Val Met Thr           
    530                 535                 540                           

ggt att acc gtc aca gct tta gaa tct aca tca aga gtc aga cct gat       1680
Gly Ile Thr Val Thr Ala Leu Glu Ser Thr Ser Arg Val Arg Pro Asp           
545                 550                 555                 560           

ggt gtt tta gtc aag cat gtt act ggt gtt cac ttg att cag gca tct       1728
Gly Val Leu Val Lys His Val Thr Gly Val His Leu Ile Gln Ala Ser           
                565                 570                 575               

ggt caa tct atg gtt ttg aat gca gac gct gtt atc tta gct act ggt       1776
Gly Gln Ser Met Val Leu Asn Ala Asp Ala Val Ile Leu Ala Thr Gly           
            580                 585                 590                   

ggt ttc tcc aat gat cat acc cca aac tcc ctt tta caa caa tac gcc       1824
Gly Phe Ser Asn Asp His Thr Pro Asn Ser Leu Leu Gln Gln Tyr Ala           
        595                 600                 605                       

cca cag ttg tca tct ttt cca aca acc aat ggt gtc tgg gca act ggc       1872
Pro Gln Leu Ser Ser Phe Pro Thr Thr Asn Gly Val Trp Ala Thr Gly           
    610                 615                 620                           

gat ggt gtt aag atg gct tcc aag ttg ggt gtc gcc tta gtt gat atg       1920
Asp Gly Val Lys Met Ala Ser Lys Leu Gly Val Ala Leu Val Asp Met           
625                 630                 635                 640           

gat aag gtc caa tta cat cct acc ggc ttg tta gac cca aaa gat cca       1968
Asp Lys Val Gln Leu His Pro Thr Gly Leu Leu Asp Pro Lys Asp Pro           
                645                 650                 655               

tct aat aga acc aag tat ctt ggt cca gag gcc tta aga ggt tcc ggc       2016
Ser Asn Arg Thr Lys Tyr Leu Gly Pro Glu Ala Leu Arg Gly Ser Gly           
            660                 665                 670                   

ggt gtc ttg tta aac aaa aac ggt gaa aga ttt gtt aat gaa tta gac       2064
Gly Val Leu Leu Asn Lys Asn Gly Glu Arg Phe Val Asn Glu Leu Asp           
        675                 680                 685                       

tta aga tct gtt gtc tct caa gct atc atc gca caa gat aat gag tac       2112
Leu Arg Ser Val Val Ser Gln Ala Ile Ile Ala Gln Asp Asn Glu Tyr           
    690                 695                 700                           

cca ggc tct ggt ggt tcc aag ttc gca tac tgt gtt ttg aac gaa act       2160
Pro Gly Ser Gly Gly Ser Lys Phe Ala Tyr Cys Val Leu Asn Glu Thr           
705                 710                 715                 720           

gca gca aag tta ttc ggc aaa aac ttc ctt ggt ttc tac tgg aat aga       2208
Ala Ala Lys Leu Phe Gly Lys Asn Phe Leu Gly Phe Tyr Trp Asn Arg           
                725                 730                 735               

tta ggt ctt ttc caa aag gtt gat tcc gtt gct ggt tta gct aag ttg       2256
Leu Gly Leu Phe Gln Lys Val Asp Ser Val Ala Gly Leu Ala Lys Leu           
            740                 745                 750                   

att ggt tgt cca gaa gct aat gtt gtt gct aca ttg aag caa tat gag       2304
Ile Gly Cys Pro Glu Ala Asn Val Val Ala Thr Leu Lys Gln Tyr Glu           
        755                 760                 765                       

gag tta tct tcc aaa aag ctt aat cct tgt cca ttg act ggc aag tct       2352
Glu Leu Ser Ser Lys Lys Leu Asn Pro Cys Pro Leu Thr Gly Lys Ser           
    770                 775                 780                           

gtc ttt cct tgt gtt tta ggc act caa ggt cca tac tat gtt gcc ttg       2400
Val Phe Pro Cys Val Leu Gly Thr Gln Gly Pro Tyr Tyr Val Ala Leu           
785                 790                 795                 800           

gtt acc cca tcc att cac tac act atg ggt ggt tgt ttg att tcc cca       2448
Val Thr Pro Ser Ile His Tyr Thr Met Gly Gly Cys Leu Ile Ser Pro           
                805                 810                 815               

tct gct gag atg caa acc att gac aac tct ggt gtt act cct gtc aga       2496
Ser Ala Glu Met Gln Thr Ile Asp Asn Ser Gly Val Thr Pro Val Arg           
            820                 825                 830                   

cgt cca atc tta ggc tta ttc ggt gct ggt gaa gtt act ggc ggt gtc       2544
Arg Pro Ile Leu Gly Leu Phe Gly Ala Gly Glu Val Thr Gly Gly Val           
        835                 840                 845                       

cat ggt ggt aac aga tta ggc ggt aac tct ttg tta gaa tgt gtt gtt       2592
His Gly Gly Asn Arg Leu Gly Gly Asn Ser Leu Leu Glu Cys Val Val           
    850                 855                 860                           

ttc ggc aag atc gct ggt gac aga gct gca acc atc ttg caa aag aaa       2640
Phe Gly Lys Ile Ala Gly Asp Arg Ala Ala Thr Ile Leu Gln Lys Lys           
865                 870                 875                 880           

aac acc ggc tta tca atg aca gaa tgg tct act gtc gtc tta aga gaa       2688
Asn Thr Gly Leu Ser Met Thr Glu Trp Ser Thr Val Val Leu Arg Glu           
                885                 890                 895               

gtt aga gaa ggt ggt gtc tat ggt gct ggt tcc aga gtt ttg agg ttt       2736
Val Arg Glu Gly Gly Val Tyr Gly Ala Gly Ser Arg Val Leu Arg Phe           
            900                 905                 910                   

aac atg cct ggt gca tta cag aga act ggt tta gct tta ggt caa ttc       2784
Asn Met Pro Gly Ala Leu Gln Arg Thr Gly Leu Ala Leu Gly Gln Phe           
        915                 920                 925                       

atc ggt atc aga ggt gat tgg gac ggt cac aga ttg atc ggt tac tat       2832
Ile Gly Ile Arg Gly Asp Trp Asp Gly His Arg Leu Ile Gly Tyr Tyr           
    930                 935                 940                           

tct cca atc act tta cct gat gat gtt ggt gtt att ggt atc tta gct       2880
Ser Pro Ile Thr Leu Pro Asp Asp Val Gly Val Ile Gly Ile Leu Ala           
945                 950                 955                 960           

aga gca gac aag ggt aga ttg gca gaa tgg att tct gca ttg cag cca       2928
Arg Ala Asp Lys Gly Arg Leu Ala Glu Trp Ile Ser Ala Leu Gln Pro           
                965                 970                 975               

ggt gac gct gtt gag atg aag gcc tgc ggt ggt ctt atc att gac aga       2976
Gly Asp Ala Val Glu Met Lys Ala Cys Gly Gly Leu Ile Ile Asp Arg           
            980                 985                 990                   

aga ttc gct gaa aga cat ttc ttt  ttc cgt ggt cat aag  atc aga aag     3024
Arg Phe Ala Glu Arg His Phe Phe  Phe Arg Gly His Lys  Ile Arg Lys         
        995                 1000                 1005                     

ttg gcc  ctt atc ggt ggt ggt  act ggt gtt gca cca  atg tta caa        3069
Leu Ala  Leu Ile Gly Gly Gly  Thr Gly Val Ala Pro  Met Leu Gln            
    1010                 1015                 1020                        

atc gtc  aga gct gct gtc aaa  aag cca ttt gtc gat  tca att gag        3114
Ile Val  Arg Ala Ala Val Lys  Lys Pro Phe Val Asp  Ser Ile Glu            
    1025                 1030                 1035                        

tcc att  cag ttc atc tat gct  gca gag gat gtt tcc  gag ctt aca        3159
Ser Ile  Gln Phe Ile Tyr Ala  Ala Glu Asp Val Ser  Glu Leu Thr            
    1040                 1045                 1050                        

tac aga  acc tta ctt gaa tct  tac gaa gag gaa tat  ggt tca gaa        3204
Tyr Arg  Thr Leu Leu Glu Ser  Tyr Glu Glu Glu Tyr  Gly Ser Glu            
    1055                 1060                 1065                        

aag ttt  aag tgt cac ttc gtt  ttg aat aac cca cca  gct caa tgg        3249
Lys Phe  Lys Cys His Phe Val  Leu Asn Asn Pro Pro  Ala Gln Trp            
    1070                 1075                 1080                        

act gac  ggt gtt ggt ttc gtt  gat act gca ttg ttg  aga tcc gca        3294
Thr Asp  Gly Val Gly Phe Val  Asp Thr Ala Leu Leu  Arg Ser Ala            
    1085                 1090                 1095                        

gtt caa  gca cca tca aat gat  ttg ctt gtt gca att  tgt ggt cca        3339
Val Gln  Ala Pro Ser Asn Asp  Leu Leu Val Ala Ile  Cys Gly Pro            
    1100                 1105                 1110                        

cca atc  atg caa aga gca gtt  aag ggt gca ttg aaa  ggt tta ggt        3384
Pro Ile  Met Gln Arg Ala Val  Lys Gly Ala Leu Lys  Gly Leu Gly            
    1115                 1120                 1125                        

tac aat  atg aat ctt gtt aga  acc gtt gac gaa act  gaa cca cca        3429
Tyr Asn  Met Asn Leu Val Arg  Thr Val Asp Glu Thr  Glu Pro Pro            
    1130                 1135                 1140                        

tca taa                                                               3435
Ser                                                                       
                                                                          


<210>  44
<211>  1144
<212>  PRT
<213>  Leishmania mexicana

<400>  44

Met Ala Asp Gly Lys Thr Ser Ala Ser Val Val Ala Val Asp Ala Glu 
1               5                   10                  15      


Arg Ala Ala Lys Glu Arg Asp Ala Ala Ala Arg Ala Met Leu Gln Gly 
            20                  25                  30          


Gly Gly Val Ser Pro Ala Gly Lys Ala Gln Leu Leu Lys Lys Gly Leu 
        35                  40                  45              


Val His Thr Val Pro Tyr Thr Leu Lys Val Val Val Ala Asp Pro Lys 
    50                  55                  60                  


Glu Met Glu Lys Ala Thr Ala Asp Ala Glu Glu Val Leu Gln Ala Ala 
65                  70                  75                  80  


Phe Gln Val Val Asp Thr Leu Leu Asn Asn Phe Asn Glu Asn Ser Glu 
                85                  90                  95      


Val Ser Arg Val Asn Arg Leu Ala Val Gly Glu Glu His Gln Met Ser 
            100                 105                 110         


Glu Thr Leu Lys His Val Met Ala Cys Cys Gln Lys Val Tyr His Ser 
        115                 120                 125             


Ser Arg Gly Val Phe Asp Pro Ala Val Gly Pro Leu Val Arg Glu Leu 
    130                 135                 140                 


Arg Glu Ala Ala His Lys Gly Lys Thr Val Pro Ala Glu Arg Val Asn 
145                 150                 155                 160 


Asp Leu Leu Ser Lys Cys Thr Leu Asn Ala Ser Phe Ser Ile Asp Met 
                165                 170                 175     


Ser Arg Gly Met Ile Ala Arg Lys His Pro Asp Ala Met Leu Asp Leu 
            180                 185                 190         


Gly Gly Val Asn Lys Gly Tyr Gly Ile Asp Tyr Ile Val Glu His Leu 
        195                 200                 205             


Asn Ser Leu Gly Tyr Asp Asp Val Phe Phe Glu Trp Gly Gly Asp Val 
    210                 215                 220                 


Arg Ala Ser Gly Lys Asn Gln Leu Ser Gln Pro Trp Ala Val Gly Ile 
225                 230                 235                 240 


Val Arg Pro Pro Ala Leu Ala Asp Ile Arg Thr Val Val Pro Glu Asp 
                245                 250                 255     


Lys Arg Ser Phe Ile Arg Val Val Arg Leu Asn Asn Glu Ala Ile Ala 
            260                 265                 270         


Thr Ser Gly Asp Tyr Glu Asn Leu Val Glu Gly Pro Gly Ser Lys Val 
        275                 280                 285             


Tyr Ser Ser Thr Phe Asn Pro Thr Ser Lys Asn Leu Leu Glu Pro Thr 
    290                 295                 300                 


Glu Ala Gly Met Ala Gln Val Ser Val Lys Cys Cys Ser Cys Ile Tyr 
305                 310                 315                 320 


Ala Asp Ala Leu Ala Thr Ala Ala Leu Leu Lys Asn Asp Pro Ala Ala 
                325                 330                 335     


Val Arg Arg Ile Leu Asp Asn Trp Arg Tyr Val Arg Asp Thr Val Thr 
            340                 345                 350         


Asp Tyr Thr Thr Tyr Thr Arg Glu Gly Glu Arg Val Ala Lys Met Leu 
        355                 360                 365             


Glu Ile Ala Thr Glu Asp Ala Glu Met Arg Ala Lys Arg Ile Lys Gly 
    370                 375                 380                 


Ser Leu Pro Ala Arg Val Ile Ile Val Gly Gly Gly Leu Ala Gly Cys 
385                 390                 395                 400 


Ser Ala Ala Ile Glu Ala Ala Asn Cys Gly Ala His Val Ile Leu Leu 
                405                 410                 415     


Glu Lys Glu Pro Lys Leu Gly Gly Asn Ser Ala Lys Ala Thr Ser Gly 
            420                 425                 430         


Ile Asn Ala Trp Gly Thr Arg Ala Gln Ala Lys Gln Gly Val Met Asp 
        435                 440                 445             


Gly Gly Lys Phe Phe Glu Arg Asp Thr His Arg Ser Gly Lys Gly Gly 
    450                 455                 460                 


Asn Cys Asp Pro Cys Leu Val Lys Thr Leu Ser Val Lys Ser Ser Asp 
465                 470                 475                 480 


Ala Val Lys Trp Leu Ser Glu Leu Gly Val Pro Leu Thr Val Leu Ser 
                485                 490                 495     


Gln Leu Gly Gly Ala Ser Arg Lys Arg Cys His Arg Ala Pro Asp Lys 
            500                 505                 510         


Ser Asp Gly Thr Pro Val Pro Val Gly Phe Thr Ile Met Lys Thr Leu 
        515                 520                 525             


Glu Asn His Ile Val Asn Asp Leu Ser Arg His Val Thr Val Met Thr 
    530                 535                 540                 


Gly Ile Thr Val Thr Ala Leu Glu Ser Thr Ser Arg Val Arg Pro Asp 
545                 550                 555                 560 


Gly Val Leu Val Lys His Val Thr Gly Val His Leu Ile Gln Ala Ser 
                565                 570                 575     


Gly Gln Ser Met Val Leu Asn Ala Asp Ala Val Ile Leu Ala Thr Gly 
            580                 585                 590         


Gly Phe Ser Asn Asp His Thr Pro Asn Ser Leu Leu Gln Gln Tyr Ala 
        595                 600                 605             


Pro Gln Leu Ser Ser Phe Pro Thr Thr Asn Gly Val Trp Ala Thr Gly 
    610                 615                 620                 


Asp Gly Val Lys Met Ala Ser Lys Leu Gly Val Ala Leu Val Asp Met 
625                 630                 635                 640 


Asp Lys Val Gln Leu His Pro Thr Gly Leu Leu Asp Pro Lys Asp Pro 
                645                 650                 655     


Ser Asn Arg Thr Lys Tyr Leu Gly Pro Glu Ala Leu Arg Gly Ser Gly 
            660                 665                 670         


Gly Val Leu Leu Asn Lys Asn Gly Glu Arg Phe Val Asn Glu Leu Asp 
        675                 680                 685             


Leu Arg Ser Val Val Ser Gln Ala Ile Ile Ala Gln Asp Asn Glu Tyr 
    690                 695                 700                 


Pro Gly Ser Gly Gly Ser Lys Phe Ala Tyr Cys Val Leu Asn Glu Thr 
705                 710                 715                 720 


Ala Ala Lys Leu Phe Gly Lys Asn Phe Leu Gly Phe Tyr Trp Asn Arg 
                725                 730                 735     


Leu Gly Leu Phe Gln Lys Val Asp Ser Val Ala Gly Leu Ala Lys Leu 
            740                 745                 750         


Ile Gly Cys Pro Glu Ala Asn Val Val Ala Thr Leu Lys Gln Tyr Glu 
        755                 760                 765             


Glu Leu Ser Ser Lys Lys Leu Asn Pro Cys Pro Leu Thr Gly Lys Ser 
    770                 775                 780                 


Val Phe Pro Cys Val Leu Gly Thr Gln Gly Pro Tyr Tyr Val Ala Leu 
785                 790                 795                 800 


Val Thr Pro Ser Ile His Tyr Thr Met Gly Gly Cys Leu Ile Ser Pro 
                805                 810                 815     


Ser Ala Glu Met Gln Thr Ile Asp Asn Ser Gly Val Thr Pro Val Arg 
            820                 825                 830         


Arg Pro Ile Leu Gly Leu Phe Gly Ala Gly Glu Val Thr Gly Gly Val 
        835                 840                 845             


His Gly Gly Asn Arg Leu Gly Gly Asn Ser Leu Leu Glu Cys Val Val 
    850                 855                 860                 


Phe Gly Lys Ile Ala Gly Asp Arg Ala Ala Thr Ile Leu Gln Lys Lys 
865                 870                 875                 880 


Asn Thr Gly Leu Ser Met Thr Glu Trp Ser Thr Val Val Leu Arg Glu 
                885                 890                 895     


Val Arg Glu Gly Gly Val Tyr Gly Ala Gly Ser Arg Val Leu Arg Phe 
            900                 905                 910         


Asn Met Pro Gly Ala Leu Gln Arg Thr Gly Leu Ala Leu Gly Gln Phe 
        915                 920                 925             


Ile Gly Ile Arg Gly Asp Trp Asp Gly His Arg Leu Ile Gly Tyr Tyr 
    930                 935                 940                 


Ser Pro Ile Thr Leu Pro Asp Asp Val Gly Val Ile Gly Ile Leu Ala 
945                 950                 955                 960 


Arg Ala Asp Lys Gly Arg Leu Ala Glu Trp Ile Ser Ala Leu Gln Pro 
                965                 970                 975     


Gly Asp Ala Val Glu Met Lys Ala Cys Gly Gly Leu Ile Ile Asp Arg 
            980                 985                 990         


Arg Phe Ala Glu Arg His Phe Phe  Phe Arg Gly His Lys  Ile Arg Lys 
        995                 1000                 1005             


Leu Ala  Leu Ile Gly Gly Gly  Thr Gly Val Ala Pro  Met Leu Gln 
    1010                 1015                 1020             


Ile Val  Arg Ala Ala Val Lys  Lys Pro Phe Val Asp  Ser Ile Glu 
    1025                 1030                 1035             


Ser Ile  Gln Phe Ile Tyr Ala  Ala Glu Asp Val Ser  Glu Leu Thr 
    1040                 1045                 1050             


Tyr Arg  Thr Leu Leu Glu Ser  Tyr Glu Glu Glu Tyr  Gly Ser Glu 
    1055                 1060                 1065             


Lys Phe  Lys Cys His Phe Val  Leu Asn Asn Pro Pro  Ala Gln Trp 
    1070                 1075                 1080             


Thr Asp  Gly Val Gly Phe Val  Asp Thr Ala Leu Leu  Arg Ser Ala 
    1085                 1090                 1095             


Val Gln  Ala Pro Ser Asn Asp  Leu Leu Val Ala Ile  Cys Gly Pro 
    1100                 1105                 1110             


Pro Ile  Met Gln Arg Ala Val  Lys Gly Ala Leu Lys  Gly Leu Gly 
    1115                 1120                 1125             


Tyr Asn  Met Asn Leu Val Arg  Thr Val Asp Glu Thr  Glu Pro Pro 
    1130                 1135                 1140             


Ser 
    


<210>  45
<211>  1317
<212>  DNA
<213>  Schizosaccharomyces pombe


<220>
<221>  CDS
<222>  (1)..(1317)

<400>  45
atg ggt gaa ttg aaa gag att ttg aaa caa aga tat cat gaa tta ctt         48
Met Gly Glu Leu Lys Glu Ile Leu Lys Gln Arg Tyr His Glu Leu Leu           
1               5                   10                  15                

gat tgg aat gtt aag gca cca cat gtc cct tta tcc cag aga ttg aag         96
Asp Trp Asn Val Lys Ala Pro His Val Pro Leu Ser Gln Arg Leu Lys           
            20                  25                  30                    

cac ttt act tgg tca tgg ttt gct tgt act atg gca acc ggt ggt gtt        144
His Phe Thr Trp Ser Trp Phe Ala Cys Thr Met Ala Thr Gly Gly Val           
        35                  40                  45                        

ggt ttg atc att ggt tcc ttc cca ttc aga ttc tac ggt ttg aac acc        192
Gly Leu Ile Ile Gly Ser Phe Pro Phe Arg Phe Tyr Gly Leu Asn Thr           
    50                  55                  60                            

att ggc aag att gtt tac atc tta caa atc ttt ttg ttt tct ctt ttt        240
Ile Gly Lys Ile Val Tyr Ile Leu Gln Ile Phe Leu Phe Ser Leu Phe           
65                  70                  75                  80            

ggc tct tgt atg ttg ttt cgt ttc atc aag tat cca tct acc att aag        288
Gly Ser Cys Met Leu Phe Arg Phe Ile Lys Tyr Pro Ser Thr Ile Lys           
                85                  90                  95                

gac tct tgg aat cat cac ttg gaa aag ttg ttt atc gca act tgt ttg        336
Asp Ser Trp Asn His His Leu Glu Lys Leu Phe Ile Ala Thr Cys Leu           
            100                 105                 110                   

tta tct att tcc aca ttc atc gac atg tta gct atc tat gct tat cca        384
Leu Ser Ile Ser Thr Phe Ile Asp Met Leu Ala Ile Tyr Ala Tyr Pro           
        115                 120                 125                       

gat acc ggt gaa tgg atg gtc tgg gtc att aga atc tta tac tac atc        432
Asp Thr Gly Glu Trp Met Val Trp Val Ile Arg Ile Leu Tyr Tyr Ile           
    130                 135                 140                           

tat gtc gct gtc tct ttc atc tac tgt gtt atg gcc ttt ttc acc att        480
Tyr Val Ala Val Ser Phe Ile Tyr Cys Val Met Ala Phe Phe Thr Ile           
145                 150                 155                 160           

ttc aac aat cat gtt tac act att gaa act gct tct cca gct tgg att        528
Phe Asn Asn His Val Tyr Thr Ile Glu Thr Ala Ser Pro Ala Trp Ile           
                165                 170                 175               

ttg cca atc ttc cct cca atg atc tgt ggt gtc att gct ggt gct gtt        576
Leu Pro Ile Phe Pro Pro Met Ile Cys Gly Val Ile Ala Gly Ala Val           
            180                 185                 190                   

aac tcc acc caa cct gct cac caa ttg aaa aac atg gtc att ttc ggt        624
Asn Ser Thr Gln Pro Ala His Gln Leu Lys Asn Met Val Ile Phe Gly           
        195                 200                 205                       

atc ttg ttt caa ggt tta ggt ttt tgg gtt tac ctt tta ctt ttc gcc        672
Ile Leu Phe Gln Gly Leu Gly Phe Trp Val Tyr Leu Leu Leu Phe Ala           
    210                 215                 220                           

gtt aat gtt ttg aga ttc ttc aca gtc ggt tta gca aag cca caa gat        720
Val Asn Val Leu Arg Phe Phe Thr Val Gly Leu Ala Lys Pro Gln Asp           
225                 230                 235                 240           

aga cca ggt atg ttt atg ttc gtt ggt cca cca gct ttc tct ggt tta        768
Arg Pro Gly Met Phe Met Phe Val Gly Pro Pro Ala Phe Ser Gly Leu           
                245                 250                 255               

gca ttg att aac att gca aga ggt gca atg ggc tca aga cct tac att        816
Ala Leu Ile Asn Ile Ala Arg Gly Ala Met Gly Ser Arg Pro Tyr Ile           
            260                 265                 270                   

ttc gtt ggt gca aac tct tcc gaa tac tta ggt ttt gtc tca acc ttc        864
Phe Val Gly Ala Asn Ser Ser Glu Tyr Leu Gly Phe Val Ser Thr Phe           
        275                 280                 285                       

atg gcc att ttc atc tgg ggt tta gcc gca tgg tgt tat tgc tta gct        912
Met Ala Ile Phe Ile Trp Gly Leu Ala Ala Trp Cys Tyr Cys Leu Ala           
    290                 295                 300                           

atg gtt tcc ttc ctt gcc ggc ttt ttc act aga gca cca ttg aaa ttc        960
Met Val Ser Phe Leu Ala Gly Phe Phe Thr Arg Ala Pro Leu Lys Phe           
305                 310                 315                 320           

gct tgt ggt tgg ttc gct ttc atc ttt cca aat gtt ggt ttt gtt aac       1008
Ala Cys Gly Trp Phe Ala Phe Ile Phe Pro Asn Val Gly Phe Val Asn           
                325                 330                 335               

tgt act atc gaa atc ggc aag atg att gat tct aag gct ttt caa atg       1056
Cys Thr Ile Glu Ile Gly Lys Met Ile Asp Ser Lys Ala Phe Gln Met           
            340                 345                 350                   

ttt ggt cac atc att ggt gtt atc ttg tgt att caa tgg att ttg tta       1104
Phe Gly His Ile Ile Gly Val Ile Leu Cys Ile Gln Trp Ile Leu Leu           
        355                 360                 365                       

atg tac tta atg gtt aga gca ttc ctt gtt aat gac ttg tgc tat cct       1152
Met Tyr Leu Met Val Arg Ala Phe Leu Val Asn Asp Leu Cys Tyr Pro           
    370                 375                 380                           

ggt aaa gac gaa gat gca cac cca cca cca aag cca aac act ggt gtc       1200
Gly Lys Asp Glu Asp Ala His Pro Pro Pro Lys Pro Asn Thr Gly Val           
385                 390                 395                 400           

tta aac cca act ttc cca cca gag aag gct cca gca tca tta gag aag       1248
Leu Asn Pro Thr Phe Pro Pro Glu Lys Ala Pro Ala Ser Leu Glu Lys           
                405                 410                 415               

gtt gat act cat gtt aca tca aca ggt ggt gaa tcc gat cct cca tct       1296
Val Asp Thr His Val Thr Ser Thr Gly Gly Glu Ser Asp Pro Pro Ser           
            420                 425                 430                   

tcc gaa cat gaa tcc gtt taa                                           1317
Ser Glu His Glu Ser Val                                                   
        435                                                               


<210>  46
<211>  438
<212>  PRT
<213>  Schizosaccharomyces pombe

<400>  46

Met Gly Glu Leu Lys Glu Ile Leu Lys Gln Arg Tyr His Glu Leu Leu 
1               5                   10                  15      


Asp Trp Asn Val Lys Ala Pro His Val Pro Leu Ser Gln Arg Leu Lys 
            20                  25                  30          


His Phe Thr Trp Ser Trp Phe Ala Cys Thr Met Ala Thr Gly Gly Val 
        35                  40                  45              


Gly Leu Ile Ile Gly Ser Phe Pro Phe Arg Phe Tyr Gly Leu Asn Thr 
    50                  55                  60                  


Ile Gly Lys Ile Val Tyr Ile Leu Gln Ile Phe Leu Phe Ser Leu Phe 
65                  70                  75                  80  


Gly Ser Cys Met Leu Phe Arg Phe Ile Lys Tyr Pro Ser Thr Ile Lys 
                85                  90                  95      


Asp Ser Trp Asn His His Leu Glu Lys Leu Phe Ile Ala Thr Cys Leu 
            100                 105                 110         


Leu Ser Ile Ser Thr Phe Ile Asp Met Leu Ala Ile Tyr Ala Tyr Pro 
        115                 120                 125             


Asp Thr Gly Glu Trp Met Val Trp Val Ile Arg Ile Leu Tyr Tyr Ile 
    130                 135                 140                 


Tyr Val Ala Val Ser Phe Ile Tyr Cys Val Met Ala Phe Phe Thr Ile 
145                 150                 155                 160 


Phe Asn Asn His Val Tyr Thr Ile Glu Thr Ala Ser Pro Ala Trp Ile 
                165                 170                 175     


Leu Pro Ile Phe Pro Pro Met Ile Cys Gly Val Ile Ala Gly Ala Val 
            180                 185                 190         


Asn Ser Thr Gln Pro Ala His Gln Leu Lys Asn Met Val Ile Phe Gly 
        195                 200                 205             


Ile Leu Phe Gln Gly Leu Gly Phe Trp Val Tyr Leu Leu Leu Phe Ala 
    210                 215                 220                 


Val Asn Val Leu Arg Phe Phe Thr Val Gly Leu Ala Lys Pro Gln Asp 
225                 230                 235                 240 


Arg Pro Gly Met Phe Met Phe Val Gly Pro Pro Ala Phe Ser Gly Leu 
                245                 250                 255     


Ala Leu Ile Asn Ile Ala Arg Gly Ala Met Gly Ser Arg Pro Tyr Ile 
            260                 265                 270         


Phe Val Gly Ala Asn Ser Ser Glu Tyr Leu Gly Phe Val Ser Thr Phe 
        275                 280                 285             


Met Ala Ile Phe Ile Trp Gly Leu Ala Ala Trp Cys Tyr Cys Leu Ala 
    290                 295                 300                 


Met Val Ser Phe Leu Ala Gly Phe Phe Thr Arg Ala Pro Leu Lys Phe 
305                 310                 315                 320 


Ala Cys Gly Trp Phe Ala Phe Ile Phe Pro Asn Val Gly Phe Val Asn 
                325                 330                 335     


Cys Thr Ile Glu Ile Gly Lys Met Ile Asp Ser Lys Ala Phe Gln Met 
            340                 345                 350         


Phe Gly His Ile Ile Gly Val Ile Leu Cys Ile Gln Trp Ile Leu Leu 
        355                 360                 365             


Met Tyr Leu Met Val Arg Ala Phe Leu Val Asn Asp Leu Cys Tyr Pro 
    370                 375                 380                 


Gly Lys Asp Glu Asp Ala His Pro Pro Pro Lys Pro Asn Thr Gly Val 
385                 390                 395                 400 


Leu Asn Pro Thr Phe Pro Pro Glu Lys Ala Pro Ala Ser Leu Glu Lys 
                405                 410                 415     


Val Asp Thr His Val Thr Ser Thr Gly Gly Glu Ser Asp Pro Pro Ser 
            420                 425                 430         


Ser Glu His Glu Ser Val 
        435             


<210>  47
<211>  1205
<212>  DNA
<213>  Aspergillus oryzae


<220>
<221>  CDS
<222>  (1)..(1197)

<400>  47
atg ttt aac aat gag cac cat att cct cct ggt tcc tct cac tct gat         48
Met Phe Asn Asn Glu His His Ile Pro Pro Gly Ser Ser His Ser Asp           
1               5                   10                  15                

atc gaa atg tta aca cca cca aag ttt gag gat gaa aaa cag tta ggt         96
Ile Glu Met Leu Thr Pro Pro Lys Phe Glu Asp Glu Lys Gln Leu Gly           
            20                  25                  30                    

cca gtc ggt att aga gaa aga ttg aga cat ttc act tgg gct tgg tat        144
Pro Val Gly Ile Arg Glu Arg Leu Arg His Phe Thr Trp Ala Trp Tyr           
        35                  40                  45                        

acc tta acc atg tcc ggt ggt ggt ttg gca gtt ttg att atc tct cag        192
Thr Leu Thr Met Ser Gly Gly Gly Leu Ala Val Leu Ile Ile Ser Gln           
    50                  55                  60                            

cca ttc ggt ttt aga ggt tta aga gaa att ggt att gca gtt tac att        240
Pro Phe Gly Phe Arg Gly Leu Arg Glu Ile Gly Ile Ala Val Tyr Ile           
65                  70                  75                  80            

ttg aac tta atc tta ttc gct ttg gtt tgt tct acc atg gct att cgt        288
Leu Asn Leu Ile Leu Phe Ala Leu Val Cys Ser Thr Met Ala Ile Arg           
                85                  90                  95                

ttc atc ttg cac ggt aac ctt ttg gaa tcc ctt aga cat gac aga gaa        336
Phe Ile Leu His Gly Asn Leu Leu Glu Ser Leu Arg His Asp Arg Glu           
            100                 105                 110                   

ggt ttg ttt ttc cct act ttc tgg ttg tct gtt gct acc atc att tgt        384
Gly Leu Phe Phe Pro Thr Phe Trp Leu Ser Val Ala Thr Ile Ile Cys           
        115                 120                 125                       

ggt ttg tca aga tac ttt ggt gag gaa tcc aac gaa tcc ttc caa ttg        432
Gly Leu Ser Arg Tyr Phe Gly Glu Glu Ser Asn Glu Ser Phe Gln Leu           
    130                 135                 140                           

gca tta gaa gcc ttg ttc tgg atc tat tgc gtt tgt acc ttg ttg gtt        480
Ala Leu Glu Ala Leu Phe Trp Ile Tyr Cys Val Cys Thr Leu Leu Val           
145                 150                 155                 160           

gca atc att caa tac tct ttt gtt ttc tca tcc cac aag tac ggt tta        528
Ala Ile Ile Gln Tyr Ser Phe Val Phe Ser Ser His Lys Tyr Gly Leu           
                165                 170                 175               

caa aca atg atg cca tct tgg att ttg cca gcc ttt cct atc atg ttg        576
Gln Thr Met Met Pro Ser Trp Ile Leu Pro Ala Phe Pro Ile Met Leu           
            180                 185                 190                   

tca ggc aca att gca tct gtt atc ggt gaa caa caa cca gcc aga gct        624
Ser Gly Thr Ile Ala Ser Val Ile Gly Glu Gln Gln Pro Ala Arg Ala           
        195                 200                 205                       

gca tta cca atc att ggt gcc ggt gtc acc ttc caa ggt tta ggt ttt        672
Ala Leu Pro Ile Ile Gly Ala Gly Val Thr Phe Gln Gly Leu Gly Phe           
    210                 215                 220                           

tct att tcc ttc atg atg tat gct cat tac att ggc aga ctt atg gaa        720
Ser Ile Ser Phe Met Met Tyr Ala His Tyr Ile Gly Arg Leu Met Glu           
225                 230                 235                 240           

tcc ggt tta cct cac tcc gac cat aga cca ggc atg ttc atc tgt gtt        768
Ser Gly Leu Pro His Ser Asp His Arg Pro Gly Met Phe Ile Cys Val           
                245                 250                 255               

ggc cca cca gcc ttt act gct ttg gct tta gtc ggt atg tcc aag ggt        816
Gly Pro Pro Ala Phe Thr Ala Leu Ala Leu Val Gly Met Ser Lys Gly           
            260                 265                 270                   

tta cca gaa gat ttc aag ctt tta cat gac gct cat gca tta gag gat        864
Leu Pro Glu Asp Phe Lys Leu Leu His Asp Ala His Ala Leu Glu Asp           
        275                 280                 285                       

ggt aga atc att gaa ttg tta gca att tca gca ggt gtt ttc ctt tgg        912
Gly Arg Ile Ile Glu Leu Leu Ala Ile Ser Ala Gly Val Phe Leu Trp           
    290                 295                 300                           

gca tta tcc ctt tgg ttt ttc tgt att gct att gtc gct gtc att aga        960
Ala Leu Ser Leu Trp Phe Phe Cys Ile Ala Ile Val Ala Val Ile Arg           
305                 310                 315                 320           

tct cca cca gaa gct ttc cac ttg aac tgg tgg gct atg gtt ttc cca       1008
Ser Pro Pro Glu Ala Phe His Leu Asn Trp Trp Ala Met Val Phe Pro           
                325                 330                 335               

aat act ggt ttc acc tta gct act atc act ttg ggt aaa gct ttg aac       1056
Asn Thr Gly Phe Thr Leu Ala Thr Ile Thr Leu Gly Lys Ala Leu Asn           
            340                 345                 350                   

tca aat ggt gtc aag ggt gtc ggt tct gca atg tcc att tgt att gtc       1104
Ser Asn Gly Val Lys Gly Val Gly Ser Ala Met Ser Ile Cys Ile Val           
        355                 360                 365                       

tgc atg tac atc ttt gtt ttc gtt aac aat gtt aga gct gtt att cgt       1152
Cys Met Tyr Ile Phe Val Phe Val Asn Asn Val Arg Ala Val Ile Arg           
    370                 375                 380                           

aag gat atc atg tat cca ggc aaa gat gag gat gtt tct gat taa           1197
Lys Asp Ile Met Tyr Pro Gly Lys Asp Glu Asp Val Ser Asp                   
385                 390                 395                               

cctgcagg                                                              1205


<210>  48
<211>  398
<212>  PRT
<213>  Aspergillus oryzae

<400>  48

Met Phe Asn Asn Glu His His Ile Pro Pro Gly Ser Ser His Ser Asp 
1               5                   10                  15      


Ile Glu Met Leu Thr Pro Pro Lys Phe Glu Asp Glu Lys Gln Leu Gly 
            20                  25                  30          


Pro Val Gly Ile Arg Glu Arg Leu Arg His Phe Thr Trp Ala Trp Tyr 
        35                  40                  45              


Thr Leu Thr Met Ser Gly Gly Gly Leu Ala Val Leu Ile Ile Ser Gln 
    50                  55                  60                  


Pro Phe Gly Phe Arg Gly Leu Arg Glu Ile Gly Ile Ala Val Tyr Ile 
65                  70                  75                  80  


Leu Asn Leu Ile Leu Phe Ala Leu Val Cys Ser Thr Met Ala Ile Arg 
                85                  90                  95      


Phe Ile Leu His Gly Asn Leu Leu Glu Ser Leu Arg His Asp Arg Glu 
            100                 105                 110         


Gly Leu Phe Phe Pro Thr Phe Trp Leu Ser Val Ala Thr Ile Ile Cys 
        115                 120                 125             


Gly Leu Ser Arg Tyr Phe Gly Glu Glu Ser Asn Glu Ser Phe Gln Leu 
    130                 135                 140                 


Ala Leu Glu Ala Leu Phe Trp Ile Tyr Cys Val Cys Thr Leu Leu Val 
145                 150                 155                 160 


Ala Ile Ile Gln Tyr Ser Phe Val Phe Ser Ser His Lys Tyr Gly Leu 
                165                 170                 175     


Gln Thr Met Met Pro Ser Trp Ile Leu Pro Ala Phe Pro Ile Met Leu 
            180                 185                 190         


Ser Gly Thr Ile Ala Ser Val Ile Gly Glu Gln Gln Pro Ala Arg Ala 
        195                 200                 205             


Ala Leu Pro Ile Ile Gly Ala Gly Val Thr Phe Gln Gly Leu Gly Phe 
    210                 215                 220                 


Ser Ile Ser Phe Met Met Tyr Ala His Tyr Ile Gly Arg Leu Met Glu 
225                 230                 235                 240 


Ser Gly Leu Pro His Ser Asp His Arg Pro Gly Met Phe Ile Cys Val 
                245                 250                 255     


Gly Pro Pro Ala Phe Thr Ala Leu Ala Leu Val Gly Met Ser Lys Gly 
            260                 265                 270         


Leu Pro Glu Asp Phe Lys Leu Leu His Asp Ala His Ala Leu Glu Asp 
        275                 280                 285             


Gly Arg Ile Ile Glu Leu Leu Ala Ile Ser Ala Gly Val Phe Leu Trp 
    290                 295                 300                 


Ala Leu Ser Leu Trp Phe Phe Cys Ile Ala Ile Val Ala Val Ile Arg 
305                 310                 315                 320 


Ser Pro Pro Glu Ala Phe His Leu Asn Trp Trp Ala Met Val Phe Pro 
                325                 330                 335     


Asn Thr Gly Phe Thr Leu Ala Thr Ile Thr Leu Gly Lys Ala Leu Asn 
            340                 345                 350         


Ser Asn Gly Val Lys Gly Val Gly Ser Ala Met Ser Ile Cys Ile Val 
        355                 360                 365             


Cys Met Tyr Ile Phe Val Phe Val Asn Asn Val Arg Ala Val Ile Arg 
    370                 375                 380                 


Lys Asp Ile Met Tyr Pro Gly Lys Asp Glu Asp Val Ser Asp 
385                 390                 395             


<210>  49
<211>  1728
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(1728)

<400>  49
atg act gac aaa atc tcc cta ggt act tat ctg ttt gaa aag tta aag         48
Met Thr Asp Lys Ile Ser Leu Gly Thr Tyr Leu Phe Glu Lys Leu Lys           
1               5                   10                  15                

gaa gca ggc tct tat tcc atc ttt ggt gtt cct ggt gat ttc aat ttg         96
Glu Ala Gly Ser Tyr Ser Ile Phe Gly Val Pro Gly Asp Phe Asn Leu           
            20                  25                  30                    

gca ttg ttg gac cac gtc aag gaa gtt gaa ggc att aga tgg gtc ggt        144
Ala Leu Leu Asp His Val Lys Glu Val Glu Gly Ile Arg Trp Val Gly           
        35                  40                  45                        

aac gct aac gag ttg aat gcc ggc tac gaa gct gat ggt tat gca aga        192
Asn Ala Asn Glu Leu Asn Ala Gly Tyr Glu Ala Asp Gly Tyr Ala Arg           
    50                  55                  60                            

atc aat gga ttt gca tcc cta atc acc acc ttt ggt gtc ggt gaa ttg        240
Ile Asn Gly Phe Ala Ser Leu Ile Thr Thr Phe Gly Val Gly Glu Leu           
65                  70                  75                  80            

tct gcc gtc aat gcc att gca ggt tct tat gct gaa cac gtc cca ttg        288
Ser Ala Val Asn Ala Ile Ala Gly Ser Tyr Ala Glu His Val Pro Leu           
                85                  90                  95                

atc cat att gtt ggt atg cct tcc ttg tct gct atg aag aac aac ttg        336
Ile His Ile Val Gly Met Pro Ser Leu Ser Ala Met Lys Asn Asn Leu           
            100                 105                 110                   

ttg tta cac cat acc ttg ggt gac aca aga ttc gac aac ttc acc gaa        384
Leu Leu His His Thr Leu Gly Asp Thr Arg Phe Asp Asn Phe Thr Glu           
        115                 120                 125                       

atg tca aag aaa atc agt gca aag gtt gaa att gtt tac gat ttg gaa        432
Met Ser Lys Lys Ile Ser Ala Lys Val Glu Ile Val Tyr Asp Leu Glu           
    130                 135                 140                           

tca gct cca aaa tta att aat aac ttg att gaa acc gct tat cac aca        480
Ser Ala Pro Lys Leu Ile Asn Asn Leu Ile Glu Thr Ala Tyr His Thr           
145                 150                 155                 160           

aag aga cca gtc tac ttg gga ctt cct tcc aac ttt gct gat gaa ttg        528
Lys Arg Pro Val Tyr Leu Gly Leu Pro Ser Asn Phe Ala Asp Glu Leu           
                165                 170                 175               

gtt cca gcg gca tta gtt aag gaa aac aag tta cat tta gaa gaa cct        576
Val Pro Ala Ala Leu Val Lys Glu Asn Lys Leu His Leu Glu Glu Pro           
            180                 185                 190                   

cta aac aac ccc gtt gct gaa gaa gaa ttc att cat aac gtt gtt gaa        624
Leu Asn Asn Pro Val Ala Glu Glu Glu Phe Ile His Asn Val Val Glu           
        195                 200                 205                       

atg gtc aag aag gca gaa aaa cca atc att ctc gtt gac gct tgt gct        672
Met Val Lys Lys Ala Glu Lys Pro Ile Ile Leu Val Asp Ala Cys Ala           
    210                 215                 220                           

gca aga cat aac att tct aag gaa gtg aga gag ttg gct aaa ttg act        720
Ala Arg His Asn Ile Ser Lys Glu Val Arg Glu Leu Ala Lys Leu Thr           
225                 230                 235                 240           

aaa ttc cct gtc ttc acc acc cca atg ggt aaa tct act gtt gat gaa        768
Lys Phe Pro Val Phe Thr Thr Pro Met Gly Lys Ser Thr Val Asp Glu           
                245                 250                 255               

gat gat gaa gaa ttc ttt ggc tta tac ttg ggt tct cta tct gct cca        816
Asp Asp Glu Glu Phe Phe Gly Leu Tyr Leu Gly Ser Leu Ser Ala Pro           
            260                 265                 270                   

gat gtt aag gac att gtt ggc cca acc gat tgt atc tta tcc tta ggt        864
Asp Val Lys Asp Ile Val Gly Pro Thr Asp Cys Ile Leu Ser Leu Gly           
        275                 280                 285                       

ggt tta cct tct gat ttc aac acc ggt tcc ttc tca tat ggt tac acc        912
Gly Leu Pro Ser Asp Phe Asn Thr Gly Ser Phe Ser Tyr Gly Tyr Thr           
    290                 295                 300                           

act aag aat gtc gtt gaa ttc cat tcc aac tac tgt aaa ttc aaa tct        960
Thr Lys Asn Val Val Glu Phe His Ser Asn Tyr Cys Lys Phe Lys Ser           
305                 310                 315                 320           

gca act tat gaa aac ttg atg atg aag ggc gca gtc caa aga ttg atc       1008
Ala Thr Tyr Glu Asn Leu Met Met Lys Gly Ala Val Gln Arg Leu Ile           
                325                 330                 335               

agc gaa ttg aag aat att aag tat tcc aat gtc tca act tta tct cca       1056
Ser Glu Leu Lys Asn Ile Lys Tyr Ser Asn Val Ser Thr Leu Ser Pro           
            340                 345                 350                   

cca aaa tct aaa ttt gct tac gaa tct gca aag gtt gct cca gaa ggt       1104
Pro Lys Ser Lys Phe Ala Tyr Glu Ser Ala Lys Val Ala Pro Glu Gly           
        355                 360                 365                       

atc atc act caa gat tac ctg tgg aag aga tta tct tac ttc tta aag       1152
Ile Ile Thr Gln Asp Tyr Leu Trp Lys Arg Leu Ser Tyr Phe Leu Lys           
    370                 375                 380                           

cca aga gat atc att gtc act gaa act ggt act tcc tcc ttt ggt gtc       1200
Pro Arg Asp Ile Ile Val Thr Glu Thr Gly Thr Ser Ser Phe Gly Val           
385                 390                 395                 400           

ttg gct acc cac tta cca aga gat tca aag tct atc tcc caa gtc tta       1248
Leu Ala Thr His Leu Pro Arg Asp Ser Lys Ser Ile Ser Gln Val Leu           
                405                 410                 415               

tgg ggt tcc att ggt ttc tcc tta cca gct gca gtt ggt gct gca ttt       1296
Trp Gly Ser Ile Gly Phe Ser Leu Pro Ala Ala Val Gly Ala Ala Phe           
            420                 425                 430                   

gct gct gaa gat gca cac aaa caa act ggc gaa caa gaa aga aga act       1344
Ala Ala Glu Asp Ala His Lys Gln Thr Gly Glu Gln Glu Arg Arg Thr           
        435                 440                 445                       

gtt ttg ttt att ggt gat ggt tct tta caa ttg act gtc caa tca atc       1392
Val Leu Phe Ile Gly Asp Gly Ser Leu Gln Leu Thr Val Gln Ser Ile           
    450                 455                 460                           

tca gat gct gca aga tgg aac atc aag cca tac atc ttc atc tta aac       1440
Ser Asp Ala Ala Arg Trp Asn Ile Lys Pro Tyr Ile Phe Ile Leu Asn           
465                 470                 475                 480           

aac aga ggt tac act atc gaa aag ttg atc cac ggt cgt cat gag gac       1488
Asn Arg Gly Tyr Thr Ile Glu Lys Leu Ile His Gly Arg His Glu Asp           
                485                 490                 495               

tac aac caa att caa cca tgg gat cac caa ttg tta ttg aag ctc ttt       1536
Tyr Asn Gln Ile Gln Pro Trp Asp His Gln Leu Leu Leu Lys Leu Phe           
            500                 505                 510                   

gct gac aag acc caa tat gaa aac cat gtt gtt aaa tcc gct aag gac       1584
Ala Asp Lys Thr Gln Tyr Glu Asn His Val Val Lys Ser Ala Lys Asp           
        515                 520                 525                       

ttg gac gct ttg atg aag gat gaa gca ttc aac aag gaa gat aag att       1632
Leu Asp Ala Leu Met Lys Asp Glu Ala Phe Asn Lys Glu Asp Lys Ile           
    530                 535                 540                           

aga gtc att gaa tta ttc ttg gat gaa ttc gat gct cca gaa atc ttg       1680
Arg Val Ile Glu Leu Phe Leu Asp Glu Phe Asp Ala Pro Glu Ile Leu           
545                 550                 555                 560           

gtt gct caa gct aaa tta tct gat gaa atc aac tct aaa gcc gct taa       1728
Val Ala Gln Ala Lys Leu Ser Asp Glu Ile Asn Ser Lys Ala Ala               
                565                 570                 575               


<210>  50
<211>  575
<212>  PRT
<213>  Issatchenkia orientalis

<400>  50

Met Thr Asp Lys Ile Ser Leu Gly Thr Tyr Leu Phe Glu Lys Leu Lys 
1               5                   10                  15      


Glu Ala Gly Ser Tyr Ser Ile Phe Gly Val Pro Gly Asp Phe Asn Leu 
            20                  25                  30          


Ala Leu Leu Asp His Val Lys Glu Val Glu Gly Ile Arg Trp Val Gly 
        35                  40                  45              


Asn Ala Asn Glu Leu Asn Ala Gly Tyr Glu Ala Asp Gly Tyr Ala Arg 
    50                  55                  60                  


Ile Asn Gly Phe Ala Ser Leu Ile Thr Thr Phe Gly Val Gly Glu Leu 
65                  70                  75                  80  


Ser Ala Val Asn Ala Ile Ala Gly Ser Tyr Ala Glu His Val Pro Leu 
                85                  90                  95      


Ile His Ile Val Gly Met Pro Ser Leu Ser Ala Met Lys Asn Asn Leu 
            100                 105                 110         


Leu Leu His His Thr Leu Gly Asp Thr Arg Phe Asp Asn Phe Thr Glu 
        115                 120                 125             


Met Ser Lys Lys Ile Ser Ala Lys Val Glu Ile Val Tyr Asp Leu Glu 
    130                 135                 140                 


Ser Ala Pro Lys Leu Ile Asn Asn Leu Ile Glu Thr Ala Tyr His Thr 
145                 150                 155                 160 


Lys Arg Pro Val Tyr Leu Gly Leu Pro Ser Asn Phe Ala Asp Glu Leu 
                165                 170                 175     


Val Pro Ala Ala Leu Val Lys Glu Asn Lys Leu His Leu Glu Glu Pro 
            180                 185                 190         


Leu Asn Asn Pro Val Ala Glu Glu Glu Phe Ile His Asn Val Val Glu 
        195                 200                 205             


Met Val Lys Lys Ala Glu Lys Pro Ile Ile Leu Val Asp Ala Cys Ala 
    210                 215                 220                 


Ala Arg His Asn Ile Ser Lys Glu Val Arg Glu Leu Ala Lys Leu Thr 
225                 230                 235                 240 


Lys Phe Pro Val Phe Thr Thr Pro Met Gly Lys Ser Thr Val Asp Glu 
                245                 250                 255     


Asp Asp Glu Glu Phe Phe Gly Leu Tyr Leu Gly Ser Leu Ser Ala Pro 
            260                 265                 270         


Asp Val Lys Asp Ile Val Gly Pro Thr Asp Cys Ile Leu Ser Leu Gly 
        275                 280                 285             


Gly Leu Pro Ser Asp Phe Asn Thr Gly Ser Phe Ser Tyr Gly Tyr Thr 
    290                 295                 300                 


Thr Lys Asn Val Val Glu Phe His Ser Asn Tyr Cys Lys Phe Lys Ser 
305                 310                 315                 320 


Ala Thr Tyr Glu Asn Leu Met Met Lys Gly Ala Val Gln Arg Leu Ile 
                325                 330                 335     


Ser Glu Leu Lys Asn Ile Lys Tyr Ser Asn Val Ser Thr Leu Ser Pro 
            340                 345                 350         


Pro Lys Ser Lys Phe Ala Tyr Glu Ser Ala Lys Val Ala Pro Glu Gly 
        355                 360                 365             


Ile Ile Thr Gln Asp Tyr Leu Trp Lys Arg Leu Ser Tyr Phe Leu Lys 
    370                 375                 380                 


Pro Arg Asp Ile Ile Val Thr Glu Thr Gly Thr Ser Ser Phe Gly Val 
385                 390                 395                 400 


Leu Ala Thr His Leu Pro Arg Asp Ser Lys Ser Ile Ser Gln Val Leu 
                405                 410                 415     


Trp Gly Ser Ile Gly Phe Ser Leu Pro Ala Ala Val Gly Ala Ala Phe 
            420                 425                 430         


Ala Ala Glu Asp Ala His Lys Gln Thr Gly Glu Gln Glu Arg Arg Thr 
        435                 440                 445             


Val Leu Phe Ile Gly Asp Gly Ser Leu Gln Leu Thr Val Gln Ser Ile 
    450                 455                 460                 


Ser Asp Ala Ala Arg Trp Asn Ile Lys Pro Tyr Ile Phe Ile Leu Asn 
465                 470                 475                 480 


Asn Arg Gly Tyr Thr Ile Glu Lys Leu Ile His Gly Arg His Glu Asp 
                485                 490                 495     


Tyr Asn Gln Ile Gln Pro Trp Asp His Gln Leu Leu Leu Lys Leu Phe 
            500                 505                 510         


Ala Asp Lys Thr Gln Tyr Glu Asn His Val Val Lys Ser Ala Lys Asp 
        515                 520                 525             


Leu Asp Ala Leu Met Lys Asp Glu Ala Phe Asn Lys Glu Asp Lys Ile 
    530                 535                 540                 


Arg Val Ile Glu Leu Phe Leu Asp Glu Phe Asp Ala Pro Glu Ile Leu 
545                 550                 555                 560 


Val Ala Gln Ala Lys Leu Ser Asp Glu Ile Asn Ser Lys Ala Ala 
                565                 570                 575 


<210>  51
<211>  1131
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(1131)

<400>  51
atg ttt gca tca acc ttc aga agt caa gct gta aga gct gca aga ttt         48
Met Phe Ala Ser Thr Phe Arg Ser Gln Ala Val Arg Ala Ala Arg Phe           
1               5                   10                  15                

act aga ttc caa tcc act ttt gcc att cct gag aag caa atg ggt gtt         96
Thr Arg Phe Gln Ser Thr Phe Ala Ile Pro Glu Lys Gln Met Gly Val           
            20                  25                  30                    

atc ttt gaa act cat ggt ggt cct tta caa tac aag gaa att cca gtt        144
Ile Phe Glu Thr His Gly Gly Pro Leu Gln Tyr Lys Glu Ile Pro Val           
        35                  40                  45                        

cca aaa cca aaa cca act gaa att tta atc aat gtt aaa tac tct ggt        192
Pro Lys Pro Lys Pro Thr Glu Ile Leu Ile Asn Val Lys Tyr Ser Gly           
    50                  55                  60                            

gtc tgc cat acc gat tta cac gca tgg aaa ggt gac tgg cca tta cca        240
Val Cys His Thr Asp Leu His Ala Trp Lys Gly Asp Trp Pro Leu Pro           
65                  70                  75                  80            

gca aag tta ccc cta gtt ggt ggt cac gaa ggt gcg ggc att gtt gtt        288
Ala Lys Leu Pro Leu Val Gly Gly His Glu Gly Ala Gly Ile Val Val           
                85                  90                  95                

gcg aaa ggt tct gca gtt acc aac ttt gag att ggc gat tat gct ggt        336
Ala Lys Gly Ser Ala Val Thr Asn Phe Glu Ile Gly Asp Tyr Ala Gly           
            100                 105                 110                   

att aag tgg tta aac ggt tca tgt atg tca tgt gaa ttc tgt gaa caa        384
Ile Lys Trp Leu Asn Gly Ser Cys Met Ser Cys Glu Phe Cys Glu Gln           
        115                 120                 125                       

ggt gat gaa tct aac tgt gaa cat gcc gat ttg agt ggt tat act cat        432
Gly Asp Glu Ser Asn Cys Glu His Ala Asp Leu Ser Gly Tyr Thr His           
    130                 135                 140                           

gat ggt tct ttc caa caa tat gcc act gct gac gct att caa gct gca        480
Asp Gly Ser Phe Gln Gln Tyr Ala Thr Ala Asp Ala Ile Gln Ala Ala           
145                 150                 155                 160           

aag atc cca aag ggt acc gac tta tct gaa gtt gcg cca att tta tgt        528
Lys Ile Pro Lys Gly Thr Asp Leu Ser Glu Val Ala Pro Ile Leu Cys           
                165                 170                 175               

gct ggt gtt act gtc tat aaa gct ttg aaa act gct gat tta aga gca        576
Ala Gly Val Thr Val Tyr Lys Ala Leu Lys Thr Ala Asp Leu Arg Ala           
            180                 185                 190                   

ggt caa tgg gtt gcg att tct ggt gcc gct ggt ggt cta ggt tct ctt        624
Gly Gln Trp Val Ala Ile Ser Gly Ala Ala Gly Gly Leu Gly Ser Leu           
        195                 200                 205                       

gct gtc caa tat gca aag gca atg ggt cta aga gtt tta ggt atc gat        672
Ala Val Gln Tyr Ala Lys Ala Met Gly Leu Arg Val Leu Gly Ile Asp           
    210                 215                 220                           

ggt ggt gaa ggt aaa aag gaa ctt ttt gaa caa tgt ggt ggt gat gtg        720
Gly Gly Glu Gly Lys Lys Glu Leu Phe Glu Gln Cys Gly Gly Asp Val           
225                 230                 235                 240           

ttt atc gat ttc acc aga tac cca aga gat gca cct gaa aag atg gtt        768
Phe Ile Asp Phe Thr Arg Tyr Pro Arg Asp Ala Pro Glu Lys Met Val           
                245                 250                 255               

gct gat att aag gct gca act aac ggt ttg ggt cca cac ggt gtt atc        816
Ala Asp Ile Lys Ala Ala Thr Asn Gly Leu Gly Pro His Gly Val Ile           
            260                 265                 270                   

aat gtc tct gtc tcc cca gct gct atc tct caa tca tgt gac tat gtt        864
Asn Val Ser Val Ser Pro Ala Ala Ile Ser Gln Ser Cys Asp Tyr Val           
        275                 280                 285                       

aga gca act ggt aag gtt gtc ctt gtc ggt atg cca tct ggt gct gtc        912
Arg Ala Thr Gly Lys Val Val Leu Val Gly Met Pro Ser Gly Ala Val           
    290                 295                 300                           

tgt aag tct gat gtc ttc act cat gtt gtt aaa tcc tta caa att aaa        960
Cys Lys Ser Asp Val Phe Thr His Val Val Lys Ser Leu Gln Ile Lys           
305                 310                 315                 320           

ggt tct tat gtt ggt aac aga gca gat acc aga gaa gct ttg gaa ttc       1008
Gly Ser Tyr Val Gly Asn Arg Ala Asp Thr Arg Glu Ala Leu Glu Phe           
                325                 330                 335               

ttt aat gaa ggt aag gtc aga tct cca atc aag gtt gtc cca tta tct       1056
Phe Asn Glu Gly Lys Val Arg Ser Pro Ile Lys Val Val Pro Leu Ser           
            340                 345                 350                   

act tta cct gaa att tac gaa ttg atg gag caa ggt aag att tta ggt       1104
Thr Leu Pro Glu Ile Tyr Glu Leu Met Glu Gln Gly Lys Ile Leu Gly           
        355                 360                 365                       

aga tac gtt gtt gat act tct aaa taa                                   1131
Arg Tyr Val Val Asp Thr Ser Lys                                           
    370                 375                                               


<210>  52
<211>  376
<212>  PRT
<213>  Issatchenkia orientalis

<400>  52

Met Phe Ala Ser Thr Phe Arg Ser Gln Ala Val Arg Ala Ala Arg Phe 
1               5                   10                  15      


Thr Arg Phe Gln Ser Thr Phe Ala Ile Pro Glu Lys Gln Met Gly Val 
            20                  25                  30          


Ile Phe Glu Thr His Gly Gly Pro Leu Gln Tyr Lys Glu Ile Pro Val 
        35                  40                  45              


Pro Lys Pro Lys Pro Thr Glu Ile Leu Ile Asn Val Lys Tyr Ser Gly 
    50                  55                  60                  


Val Cys His Thr Asp Leu His Ala Trp Lys Gly Asp Trp Pro Leu Pro 
65                  70                  75                  80  


Ala Lys Leu Pro Leu Val Gly Gly His Glu Gly Ala Gly Ile Val Val 
                85                  90                  95      


Ala Lys Gly Ser Ala Val Thr Asn Phe Glu Ile Gly Asp Tyr Ala Gly 
            100                 105                 110         


Ile Lys Trp Leu Asn Gly Ser Cys Met Ser Cys Glu Phe Cys Glu Gln 
        115                 120                 125             


Gly Asp Glu Ser Asn Cys Glu His Ala Asp Leu Ser Gly Tyr Thr His 
    130                 135                 140                 


Asp Gly Ser Phe Gln Gln Tyr Ala Thr Ala Asp Ala Ile Gln Ala Ala 
145                 150                 155                 160 


Lys Ile Pro Lys Gly Thr Asp Leu Ser Glu Val Ala Pro Ile Leu Cys 
                165                 170                 175     


Ala Gly Val Thr Val Tyr Lys Ala Leu Lys Thr Ala Asp Leu Arg Ala 
            180                 185                 190         


Gly Gln Trp Val Ala Ile Ser Gly Ala Ala Gly Gly Leu Gly Ser Leu 
        195                 200                 205             


Ala Val Gln Tyr Ala Lys Ala Met Gly Leu Arg Val Leu Gly Ile Asp 
    210                 215                 220                 


Gly Gly Glu Gly Lys Lys Glu Leu Phe Glu Gln Cys Gly Gly Asp Val 
225                 230                 235                 240 


Phe Ile Asp Phe Thr Arg Tyr Pro Arg Asp Ala Pro Glu Lys Met Val 
                245                 250                 255     


Ala Asp Ile Lys Ala Ala Thr Asn Gly Leu Gly Pro His Gly Val Ile 
            260                 265                 270         


Asn Val Ser Val Ser Pro Ala Ala Ile Ser Gln Ser Cys Asp Tyr Val 
        275                 280                 285             


Arg Ala Thr Gly Lys Val Val Leu Val Gly Met Pro Ser Gly Ala Val 
    290                 295                 300                 


Cys Lys Ser Asp Val Phe Thr His Val Val Lys Ser Leu Gln Ile Lys 
305                 310                 315                 320 


Gly Ser Tyr Val Gly Asn Arg Ala Asp Thr Arg Glu Ala Leu Glu Phe 
                325                 330                 335     


Phe Asn Glu Gly Lys Val Arg Ser Pro Ile Lys Val Val Pro Leu Ser 
            340                 345                 350         


Thr Leu Pro Glu Ile Tyr Glu Leu Met Glu Gln Gly Lys Ile Leu Gly 
        355                 360                 365             


Arg Tyr Val Val Asp Thr Ser Lys 
    370                 375     


<210>  53
<211>  1167
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(1167)

<400>  53
atg gtg tcc cct gct gaa aga tta tct act att gcg tcc aca atc aag         48
Met Val Ser Pro Ala Glu Arg Leu Ser Thr Ile Ala Ser Thr Ile Lys           
1               5                   10                  15                

cca aac aga aaa gat tct aca tca tta caa cca gaa gac tat ccg gaa         96
Pro Asn Arg Lys Asp Ser Thr Ser Leu Gln Pro Glu Asp Tyr Pro Glu           
            20                  25                  30                    

cat ccg ttc aag gtg acg gtt gtt ggt tcc ggt aac tgg ggg tgt aca        144
His Pro Phe Lys Val Thr Val Val Gly Ser Gly Asn Trp Gly Cys Thr           
        35                  40                  45                        

att gcc aag gtt ata gcg gaa aac acc gtt gag aga cct cgt caa ttt        192
Ile Ala Lys Val Ile Ala Glu Asn Thr Val Glu Arg Pro Arg Gln Phe           
    50                  55                  60                            

caa aga gat gtt aat atg tgg gtc tat gaa gaa ttg att gaa ggc gaa        240
Gln Arg Asp Val Asn Met Trp Val Tyr Glu Glu Leu Ile Glu Gly Glu           
65                  70                  75                  80            

aag ttg act gaa atc ata aat acc aaa cac gaa aac gtc aag tac ttg        288
Lys Leu Thr Glu Ile Ile Asn Thr Lys His Glu Asn Val Lys Tyr Leu           
                85                  90                  95                

cca ggt atc aag ttg cca gtt aac gtt gtt gca gtt cca gac att gtt        336
Pro Gly Ile Lys Leu Pro Val Asn Val Val Ala Val Pro Asp Ile Val           
            100                 105                 110                   

gag gct tgt gca ggc tca gac ttg att gtc ttt aat att cct cac caa        384
Glu Ala Cys Ala Gly Ser Asp Leu Ile Val Phe Asn Ile Pro His Gln           
        115                 120                 125                       

ttt tta cca aga att tta tcc caa tta aag ggt aag gtg aat cca aag        432
Phe Leu Pro Arg Ile Leu Ser Gln Leu Lys Gly Lys Val Asn Pro Lys           
    130                 135                 140                           

gct aga gca att tct tgt ttg aaa ggt ttg gat gtc aat cct aat gga        480
Ala Arg Ala Ile Ser Cys Leu Lys Gly Leu Asp Val Asn Pro Asn Gly           
145                 150                 155                 160           

tgt aag ttg ctc tcc act gtt att act gaa gag ttg ggt att tat tgt        528
Cys Lys Leu Leu Ser Thr Val Ile Thr Glu Glu Leu Gly Ile Tyr Cys           
                165                 170                 175               

ggt gcc tta tca ggt gct aat tta gct cct gaa gtt gca caa tgt aaa        576
Gly Ala Leu Ser Gly Ala Asn Leu Ala Pro Glu Val Ala Gln Cys Lys           
            180                 185                 190                   

tgg tcg gaa aca act gtt gca tat aca att ccg gac gat ttc aga ggt        624
Trp Ser Glu Thr Thr Val Ala Tyr Thr Ile Pro Asp Asp Phe Arg Gly           
        195                 200                 205                       

aaa ggc aag gat att gac cat caa att cta aag agt ttg ttc cat aga        672
Lys Gly Lys Asp Ile Asp His Gln Ile Leu Lys Ser Leu Phe His Arg           
    210                 215                 220                           

cct tat ttc cat gtt cgt gtt att agt gat gtt gca ggt att tcc att        720
Pro Tyr Phe His Val Arg Val Ile Ser Asp Val Ala Gly Ile Ser Ile           
225                 230                 235                 240           

gcc ggt gca ctc aag aat gtc gtt gct atg gct gct gga ttt gtc gaa        768
Ala Gly Ala Leu Lys Asn Val Val Ala Met Ala Ala Gly Phe Val Glu           
                245                 250                 255               

ggt tta ggt tgg ggt gat aat gca aag gct gca gtc atg aga ata ggt        816
Gly Leu Gly Trp Gly Asp Asn Ala Lys Ala Ala Val Met Arg Ile Gly           
            260                 265                 270                   

ttg gtg gaa acc att caa ttt gcc aag act ttt ttc gat ggc tgt cat        864
Leu Val Glu Thr Ile Gln Phe Ala Lys Thr Phe Phe Asp Gly Cys His           
        275                 280                 285                       

gct gca acc ttt act cat gaa tct gca ggt gtt gcc gac cta atc act        912
Ala Ala Thr Phe Thr His Glu Ser Ala Gly Val Ala Asp Leu Ile Thr           
    290                 295                 300                           

acc tgt gcc ggc ggc cgt aac gtt aga gtt ggt aga tat atg gca caa        960
Thr Cys Ala Gly Gly Arg Asn Val Arg Val Gly Arg Tyr Met Ala Gln           
305                 310                 315                 320           

cat tct gtc tct gca acg gag gct gaa gaa aag ttg ttg aat ggc caa       1008
His Ser Val Ser Ala Thr Glu Ala Glu Glu Lys Leu Leu Asn Gly Gln           
                325                 330                 335               

tcc tgt caa ggt atc cac aca act agg gaa gtt tac gag ttc ctc tcc       1056
Ser Cys Gln Gly Ile His Thr Thr Arg Glu Val Tyr Glu Phe Leu Ser           
            340                 345                 350                   

aac atg ggc agg aca gat gag ttc cca cta ttt acc acc acc tac cgt       1104
Asn Met Gly Arg Thr Asp Glu Phe Pro Leu Phe Thr Thr Thr Tyr Arg           
        355                 360                 365                       

atc atc tac gaa aac ttc cca att gag aag ctg cca gaa tgc ctt gaa       1152
Ile Ile Tyr Glu Asn Phe Pro Ile Glu Lys Leu Pro Glu Cys Leu Glu           
    370                 375                 380                           

cct gtg gaa gat taa                                                   1167
Pro Val Glu Asp                                                           
385                                                                       


<210>  54
<211>  388
<212>  PRT
<213>  Issatchenkia orientalis

<400>  54

Met Val Ser Pro Ala Glu Arg Leu Ser Thr Ile Ala Ser Thr Ile Lys 
1               5                   10                  15      


Pro Asn Arg Lys Asp Ser Thr Ser Leu Gln Pro Glu Asp Tyr Pro Glu 
            20                  25                  30          


His Pro Phe Lys Val Thr Val Val Gly Ser Gly Asn Trp Gly Cys Thr 
        35                  40                  45              


Ile Ala Lys Val Ile Ala Glu Asn Thr Val Glu Arg Pro Arg Gln Phe 
    50                  55                  60                  


Gln Arg Asp Val Asn Met Trp Val Tyr Glu Glu Leu Ile Glu Gly Glu 
65                  70                  75                  80  


Lys Leu Thr Glu Ile Ile Asn Thr Lys His Glu Asn Val Lys Tyr Leu 
                85                  90                  95      


Pro Gly Ile Lys Leu Pro Val Asn Val Val Ala Val Pro Asp Ile Val 
            100                 105                 110         


Glu Ala Cys Ala Gly Ser Asp Leu Ile Val Phe Asn Ile Pro His Gln 
        115                 120                 125             


Phe Leu Pro Arg Ile Leu Ser Gln Leu Lys Gly Lys Val Asn Pro Lys 
    130                 135                 140                 


Ala Arg Ala Ile Ser Cys Leu Lys Gly Leu Asp Val Asn Pro Asn Gly 
145                 150                 155                 160 


Cys Lys Leu Leu Ser Thr Val Ile Thr Glu Glu Leu Gly Ile Tyr Cys 
                165                 170                 175     


Gly Ala Leu Ser Gly Ala Asn Leu Ala Pro Glu Val Ala Gln Cys Lys 
            180                 185                 190         


Trp Ser Glu Thr Thr Val Ala Tyr Thr Ile Pro Asp Asp Phe Arg Gly 
        195                 200                 205             


Lys Gly Lys Asp Ile Asp His Gln Ile Leu Lys Ser Leu Phe His Arg 
    210                 215                 220                 


Pro Tyr Phe His Val Arg Val Ile Ser Asp Val Ala Gly Ile Ser Ile 
225                 230                 235                 240 


Ala Gly Ala Leu Lys Asn Val Val Ala Met Ala Ala Gly Phe Val Glu 
                245                 250                 255     


Gly Leu Gly Trp Gly Asp Asn Ala Lys Ala Ala Val Met Arg Ile Gly 
            260                 265                 270         


Leu Val Glu Thr Ile Gln Phe Ala Lys Thr Phe Phe Asp Gly Cys His 
        275                 280                 285             


Ala Ala Thr Phe Thr His Glu Ser Ala Gly Val Ala Asp Leu Ile Thr 
    290                 295                 300                 


Thr Cys Ala Gly Gly Arg Asn Val Arg Val Gly Arg Tyr Met Ala Gln 
305                 310                 315                 320 


His Ser Val Ser Ala Thr Glu Ala Glu Glu Lys Leu Leu Asn Gly Gln 
                325                 330                 335     


Ser Cys Gln Gly Ile His Thr Thr Arg Glu Val Tyr Glu Phe Leu Ser 
            340                 345                 350         


Asn Met Gly Arg Thr Asp Glu Phe Pro Leu Phe Thr Thr Thr Tyr Arg 
        355                 360                 365             


Ile Ile Tyr Glu Asn Phe Pro Ile Glu Lys Leu Pro Glu Cys Leu Glu 
    370                 375                 380                 


Pro Val Glu Asp 
385             


<210>  55
<211>  1683
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(1683)

<400>  55
atg gct cca act gct gtt gat atc cat aac gag tac aaa cag aat gtt         48
Met Ala Pro Thr Ala Val Asp Ile His Asn Glu Tyr Lys Gln Asn Val           
1               5                   10                  15                

tcc aac gaa cag gaa att cct ttc aac aaa act gaa aga aag tca tcg         96
Ser Asn Glu Gln Glu Ile Pro Phe Asn Lys Thr Glu Arg Lys Ser Ser           
            20                  25                  30                    

att gca tct aaa tta gga ctg aat cca gac gct aag att cac tac aat        144
Ile Ala Ser Lys Leu Gly Leu Asn Pro Asp Ala Lys Ile His Tyr Asn           
        35                  40                  45                        

tct gct gtt cct ata tta tac gaa gat ggt tta aag gaa aaa ggt aca        192
Ser Ala Val Pro Ile Leu Tyr Glu Asp Gly Leu Lys Glu Lys Gly Thr           
    50                  55                  60                            

acc att tcc tct tct ggt gca ttg att gca ttc tct ggt tcc aaa aca        240
Thr Ile Ser Ser Ser Gly Ala Leu Ile Ala Phe Ser Gly Ser Lys Thr           
65                  70                  75                  80            

ggt aga tct cca aag gac aaa aga att gtc gat gaa gag act tca aca        288
Gly Arg Ser Pro Lys Asp Lys Arg Ile Val Asp Glu Glu Thr Ser Thr           
                85                  90                  95                

gac aac atc tgg tgg ggt cca gtc aat aag aag gtt gat gaa aac act        336
Asp Asn Ile Trp Trp Gly Pro Val Asn Lys Lys Val Asp Glu Asn Thr           
            100                 105                 110                   

tgg aat atc tcg aaa tct aga gcg att gat tat ttg aga aca aga gag        384
Trp Asn Ile Ser Lys Ser Arg Ala Ile Asp Tyr Leu Arg Thr Arg Glu           
        115                 120                 125                       

aag gtt tac att atc gat gct ttt gct ggt tgg gat cca aga tac aga        432
Lys Val Tyr Ile Ile Asp Ala Phe Ala Gly Trp Asp Pro Arg Tyr Arg           
    130                 135                 140                           

att aag gtt aga att gtc tgt gct aga gct tac cat gct ttg ttc atg        480
Ile Lys Val Arg Ile Val Cys Ala Arg Ala Tyr His Ala Leu Phe Met           
145                 150                 155                 160           

aag aat atg tta att aga cca aca acg gaa gaa tta aag aac ttt ggt        528
Lys Asn Met Leu Ile Arg Pro Thr Thr Glu Glu Leu Lys Asn Phe Gly           
                165                 170                 175               

gag cct gat ttc acc att tgg aat gca ggt caa ttc cct gct aat gtt        576
Glu Pro Asp Phe Thr Ile Trp Asn Ala Gly Gln Phe Pro Ala Asn Val           
            180                 185                 190                   

tac act aag ggt atg act tct tca act tct gtt gaa ata aat ttc aag        624
Tyr Thr Lys Gly Met Thr Ser Ser Thr Ser Val Glu Ile Asn Phe Lys           
        195                 200                 205                       

tct atg gaa atg gtt atc cta ggt act gaa tac gca ggt gaa atg aag        672
Ser Met Glu Met Val Ile Leu Gly Thr Glu Tyr Ala Gly Glu Met Lys           
    210                 215                 220                           

aaa ggt atc ttt acc gtt atg ttc tac ttg atg cca atc aga cac aag        720
Lys Gly Ile Phe Thr Val Met Phe Tyr Leu Met Pro Ile Arg His Lys           
225                 230                 235                 240           

gtt tta act tta cac tct tct gca aat caa ggt aaa aag gat ggt gat        768
Val Leu Thr Leu His Ser Ser Ala Asn Gln Gly Lys Lys Asp Gly Asp           
                245                 250                 255               

gtc aca tta ttc ttt ggt tta tct ggt aca ggt aaa aca acc ttg tct        816
Val Thr Leu Phe Phe Gly Leu Ser Gly Thr Gly Lys Thr Thr Leu Ser           
            260                 265                 270                   

gca gat cct cat aga gaa ttg att ggt gat gat gaa cat tgc tgg tct        864
Ala Asp Pro His Arg Glu Leu Ile Gly Asp Asp Glu His Cys Trp Ser           
        275                 280                 285                       

gat cat ggt gtt ttc aac att gaa ggt gga tgt tat gct aag tgt ttg        912
Asp His Gly Val Phe Asn Ile Glu Gly Gly Cys Tyr Ala Lys Cys Leu           
    290                 295                 300                           

gac tta tct gct gaa aga gaa cct gag att ttc aat gca att agg ttt        960
Asp Leu Ser Ala Glu Arg Glu Pro Glu Ile Phe Asn Ala Ile Arg Phe           
305                 310                 315                 320           

gga tct gtc ttg gag aat gtt gtc tat gat cca gtt gat aga act gtt       1008
Gly Ser Val Leu Glu Asn Val Val Tyr Asp Pro Val Asp Arg Thr Val           
                325                 330                 335               

gac tat tcc gct gct aat gtc act gaa aat act aga tgt gct tat cct       1056
Asp Tyr Ser Ala Ala Asn Val Thr Glu Asn Thr Arg Cys Ala Tyr Pro           
            340                 345                 350                   

atc gac ttt att cct tct gct aag atc cca tgt ctg gca gat tct cat       1104
Ile Asp Phe Ile Pro Ser Ala Lys Ile Pro Cys Leu Ala Asp Ser His           
        355                 360                 365                       

cca aag aat att gtt ctt tta act tgt gat gca aga ggt gtt ttg cca       1152
Pro Lys Asn Ile Val Leu Leu Thr Cys Asp Ala Arg Gly Val Leu Pro           
    370                 375                 380                           

cct gtc tcc aag cta act aat gca caa gtc atg tat cac ttt atc tct       1200
Pro Val Ser Lys Leu Thr Asn Ala Gln Val Met Tyr His Phe Ile Ser           
385                 390                 395                 400           

ggt tac acc tcc aag atg gca ggt acc gaa gtt ggt gtc act gaa cca       1248
Gly Tyr Thr Ser Lys Met Ala Gly Thr Glu Val Gly Val Thr Glu Pro           
                405                 410                 415               

gaa gca acc ttc tct gca tgt ttt ggt caa cct ttc tta gtt tta cat       1296
Glu Ala Thr Phe Ser Ala Cys Phe Gly Gln Pro Phe Leu Val Leu His           
            420                 425                 430                   

cca atg aaa tac gca caa caa ctc tct gat aaa atg gct gaa cat tct       1344
Pro Met Lys Tyr Ala Gln Gln Leu Ser Asp Lys Met Ala Glu His Ser           
        435                 440                 445                       

tcc acc gct tgg tta ttg aat acc ggt tgg act ggt caa tct tat gtt       1392
Ser Thr Ala Trp Leu Leu Asn Thr Gly Trp Thr Gly Gln Ser Tyr Val           
    450                 455                 460                           

aaa ggt ggt aag aga tgt cca ttg aag tat act aga gca att tta gat       1440
Lys Gly Gly Lys Arg Cys Pro Leu Lys Tyr Thr Arg Ala Ile Leu Asp           
465                 470                 475                 480           

gct att cac tct ggt gag ctt gca aaa cag gaa ttc gaa aca tac cct       1488
Ala Ile His Ser Gly Glu Leu Ala Lys Gln Glu Phe Glu Thr Tyr Pro           
                485                 490                 495               

act ttc ggt tta caa gtt cca aaa act tgt cca ggt gtc cca gaa agt       1536
Thr Phe Gly Leu Gln Val Pro Lys Thr Cys Pro Gly Val Pro Glu Ser           
            500                 505                 510                   

gtt ctg aac cca tct aaa cac tgg gct act ggt gaa gct gat ttc aag       1584
Val Leu Asn Pro Ser Lys His Trp Ala Thr Gly Glu Ala Asp Phe Lys           
        515                 520                 525                       

gct gaa gtc act aac ttg gct aaa tta ttt gct gag aac ttt gaa aag       1632
Ala Glu Val Thr Asn Leu Ala Lys Leu Phe Ala Glu Asn Phe Glu Lys           
    530                 535                 540                           

tat tct gca gaa tgt act gca gaa gtt gtt gct gct ggt cct gct tta       1680
Tyr Ser Ala Glu Cys Thr Ala Glu Val Val Ala Ala Gly Pro Ala Leu           
545                 550                 555                 560           

taa                                                                   1683


<210>  56
<211>  560
<212>  PRT
<213>  Issatchenkia orientalis

<400>  56

Met Ala Pro Thr Ala Val Asp Ile His Asn Glu Tyr Lys Gln Asn Val 
1               5                   10                  15      


Ser Asn Glu Gln Glu Ile Pro Phe Asn Lys Thr Glu Arg Lys Ser Ser 
            20                  25                  30          


Ile Ala Ser Lys Leu Gly Leu Asn Pro Asp Ala Lys Ile His Tyr Asn 
        35                  40                  45              


Ser Ala Val Pro Ile Leu Tyr Glu Asp Gly Leu Lys Glu Lys Gly Thr 
    50                  55                  60                  


Thr Ile Ser Ser Ser Gly Ala Leu Ile Ala Phe Ser Gly Ser Lys Thr 
65                  70                  75                  80  


Gly Arg Ser Pro Lys Asp Lys Arg Ile Val Asp Glu Glu Thr Ser Thr 
                85                  90                  95      


Asp Asn Ile Trp Trp Gly Pro Val Asn Lys Lys Val Asp Glu Asn Thr 
            100                 105                 110         


Trp Asn Ile Ser Lys Ser Arg Ala Ile Asp Tyr Leu Arg Thr Arg Glu 
        115                 120                 125             


Lys Val Tyr Ile Ile Asp Ala Phe Ala Gly Trp Asp Pro Arg Tyr Arg 
    130                 135                 140                 


Ile Lys Val Arg Ile Val Cys Ala Arg Ala Tyr His Ala Leu Phe Met 
145                 150                 155                 160 


Lys Asn Met Leu Ile Arg Pro Thr Thr Glu Glu Leu Lys Asn Phe Gly 
                165                 170                 175     


Glu Pro Asp Phe Thr Ile Trp Asn Ala Gly Gln Phe Pro Ala Asn Val 
            180                 185                 190         


Tyr Thr Lys Gly Met Thr Ser Ser Thr Ser Val Glu Ile Asn Phe Lys 
        195                 200                 205             


Ser Met Glu Met Val Ile Leu Gly Thr Glu Tyr Ala Gly Glu Met Lys 
    210                 215                 220                 


Lys Gly Ile Phe Thr Val Met Phe Tyr Leu Met Pro Ile Arg His Lys 
225                 230                 235                 240 


Val Leu Thr Leu His Ser Ser Ala Asn Gln Gly Lys Lys Asp Gly Asp 
                245                 250                 255     


Val Thr Leu Phe Phe Gly Leu Ser Gly Thr Gly Lys Thr Thr Leu Ser 
            260                 265                 270         


Ala Asp Pro His Arg Glu Leu Ile Gly Asp Asp Glu His Cys Trp Ser 
        275                 280                 285             


Asp His Gly Val Phe Asn Ile Glu Gly Gly Cys Tyr Ala Lys Cys Leu 
    290                 295                 300                 


Asp Leu Ser Ala Glu Arg Glu Pro Glu Ile Phe Asn Ala Ile Arg Phe 
305                 310                 315                 320 


Gly Ser Val Leu Glu Asn Val Val Tyr Asp Pro Val Asp Arg Thr Val 
                325                 330                 335     


Asp Tyr Ser Ala Ala Asn Val Thr Glu Asn Thr Arg Cys Ala Tyr Pro 
            340                 345                 350         


Ile Asp Phe Ile Pro Ser Ala Lys Ile Pro Cys Leu Ala Asp Ser His 
        355                 360                 365             


Pro Lys Asn Ile Val Leu Leu Thr Cys Asp Ala Arg Gly Val Leu Pro 
    370                 375                 380                 


Pro Val Ser Lys Leu Thr Asn Ala Gln Val Met Tyr His Phe Ile Ser 
385                 390                 395                 400 


Gly Tyr Thr Ser Lys Met Ala Gly Thr Glu Val Gly Val Thr Glu Pro 
                405                 410                 415     


Glu Ala Thr Phe Ser Ala Cys Phe Gly Gln Pro Phe Leu Val Leu His 
            420                 425                 430         


Pro Met Lys Tyr Ala Gln Gln Leu Ser Asp Lys Met Ala Glu His Ser 
        435                 440                 445             


Ser Thr Ala Trp Leu Leu Asn Thr Gly Trp Thr Gly Gln Ser Tyr Val 
    450                 455                 460                 


Lys Gly Gly Lys Arg Cys Pro Leu Lys Tyr Thr Arg Ala Ile Leu Asp 
465                 470                 475                 480 


Ala Ile His Ser Gly Glu Leu Ala Lys Gln Glu Phe Glu Thr Tyr Pro 
                485                 490                 495     


Thr Phe Gly Leu Gln Val Pro Lys Thr Cys Pro Gly Val Pro Glu Ser 
            500                 505                 510         


Val Leu Asn Pro Ser Lys His Trp Ala Thr Gly Glu Ala Asp Phe Lys 
        515                 520                 525             


Ala Glu Val Thr Asn Leu Ala Lys Leu Phe Ala Glu Asn Phe Glu Lys 
    530                 535                 540                 


Tyr Ser Ala Glu Cys Thr Ala Glu Val Val Ala Ala Gly Pro Ala Leu 
545                 550                 555                 560 


<210>  57
<211>  1812
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(1812)

<400>  57
atg aca aaa gat tgc tgt gag gtt aca aag att cca gtg gga gag gag         48
Met Thr Lys Asp Cys Cys Glu Val Thr Lys Ile Pro Val Gly Glu Glu           
1               5                   10                  15                

gcc aaa gtg acc gtt cct cgt tcc aca aga tta tca gca act ggt cca         96
Ala Lys Val Thr Val Pro Arg Ser Thr Arg Leu Ser Ala Thr Gly Pro           
            20                  25                  30                    

gtt gaa tgt gat tta tct ggt ttc caa gtt ttg aac tct cca ctt ttc        144
Val Glu Cys Asp Leu Ser Gly Phe Gln Val Leu Asn Ser Pro Leu Phe           
        35                  40                  45                        

aac aag ggc act gca ttc act att gca gaa aga gaa gca ttt ggt tta        192
Asn Lys Gly Thr Ala Phe Thr Ile Ala Glu Arg Glu Ala Phe Gly Leu           
    50                  55                  60                            

aac ggg tta ctt cct ccg gtt gta aac act cta gaa gaa caa gtt gag        240
Asn Gly Leu Leu Pro Pro Val Val Asn Thr Leu Glu Glu Gln Val Glu           
65                  70                  75                  80            

aga agc tat aag caa cta cat ttt ctc aag act cca ttg gca aag aat        288
Arg Ser Tyr Lys Gln Leu His Phe Leu Lys Thr Pro Leu Ala Lys Asn           
                85                  90                  95                

gac ttt tgc acg tca ttg aga ttg caa aac aag gtt ctg ttt tat agg        336
Asp Phe Cys Thr Ser Leu Arg Leu Gln Asn Lys Val Leu Phe Tyr Arg           
            100                 105                 110                   

tta gtc aag gaa cac att aag gag ttg att cca att gtg tat aca cca        384
Leu Val Lys Glu His Ile Lys Glu Leu Ile Pro Ile Val Tyr Thr Pro           
        115                 120                 125                       

aca gag ggt gat gca att atc gct tat tct gac aga ttc aga aaa cca        432
Thr Glu Gly Asp Ala Ile Ile Ala Tyr Ser Asp Arg Phe Arg Lys Pro           
    130                 135                 140                           

gag ggg tta ttc ctt gat att aca aga cca aat gaa att gat caa aga        480
Glu Gly Leu Phe Leu Asp Ile Thr Arg Pro Asn Glu Ile Asp Gln Arg           
145                 150                 155                 160           

ctg gaa cag ttt gga gaa gat aaa gat gtg gat tac att gtt ata aca        528
Leu Glu Gln Phe Gly Glu Asp Lys Asp Val Asp Tyr Ile Val Ile Thr           
                165                 170                 175               

gat tct gaa ggt att cta ggt att ggt gac caa ggt gtt ggc ggt gtc        576
Asp Ser Glu Gly Ile Leu Gly Ile Gly Asp Gln Gly Val Gly Gly Val           
            180                 185                 190                   

aga atc aca att gca aag gct gct cta atg act gta tgt gct ggt ttg        624
Arg Ile Thr Ile Ala Lys Ala Ala Leu Met Thr Val Cys Ala Gly Leu           
        195                 200                 205                       

cat cca ggt aga gtt gtc tct tgt gtc ttg gac gtt ggc act aac aat        672
His Pro Gly Arg Val Val Ser Cys Val Leu Asp Val Gly Thr Asn Asn           
    210                 215                 220                           

gtg aaa ttg cta gaa gat gat cta tat ctt ggt aac aga ttc cca aga        720
Val Lys Leu Leu Glu Asp Asp Leu Tyr Leu Gly Asn Arg Phe Pro Arg           
225                 230                 235                 240           

gtt aga ggt aag gag tat gac gat ttt gtg aat aaa act att cgt gca        768
Val Arg Gly Lys Glu Tyr Asp Asp Phe Val Asn Lys Thr Ile Arg Ala           
                245                 250                 255               

atg aag aag aga ttt cca agt gct gtt att cat ttt gaa gat ttt ggt        816
Met Lys Lys Arg Phe Pro Ser Ala Val Ile His Phe Glu Asp Phe Gly           
            260                 265                 270                   

gtt aca act gct aga cct gtg ttg gaa aga ttc aga gat gaa att cct        864
Val Thr Thr Ala Arg Pro Val Leu Glu Arg Phe Arg Asp Glu Ile Pro           
        275                 280                 285                       

tgc ttt aat gat gac atc caa ggt acc ggc gct gtt gtc atg gct tcg        912
Cys Phe Asn Asp Asp Ile Gln Gly Thr Gly Ala Val Val Met Ala Ser           
    290                 295                 300                           

atg gcg gct gct ctt aaa tta acc cat aga aat cta cta gac tcc aaa        960
Met Ala Ala Ala Leu Lys Leu Thr His Arg Asn Leu Leu Asp Ser Lys           
305                 310                 315                 320           

gtt ttg att tat ggg gct ggt tca gcc ggt ttg ggt att gcc gat caa       1008
Val Leu Ile Tyr Gly Ala Gly Ser Ala Gly Leu Gly Ile Ala Asp Gln           
                325                 330                 335               

att gtt aat cat atg gtt agc cat ggt gcg act aaa gaa gaa gct aga       1056
Ile Val Asn His Met Val Ser His Gly Ala Thr Lys Glu Glu Ala Arg           
            340                 345                 350                   

agg aag att tac tgt atg gac agg tat ggt ttg att tta aaa ggt atg       1104
Arg Lys Ile Tyr Cys Met Asp Arg Tyr Gly Leu Ile Leu Lys Gly Met           
        355                 360                 365                       

act tcg aat tct cct gct caa gaa gat tat gct cat gat cca aaa gat       1152
Thr Ser Asn Ser Pro Ala Gln Glu Asp Tyr Ala His Asp Pro Lys Asp           
    370                 375                 380                           

tgg gaa aat att tca acc act tcg tta gtg gat gtt atc gaa aaa gtc       1200
Trp Glu Asn Ile Ser Thr Thr Ser Leu Val Asp Val Ile Glu Lys Val           
385                 390                 395                 400           

aag cct act act tta gtt ggg tgc tcc acg caa gcg ggc gct ttc aat       1248
Lys Pro Thr Thr Leu Val Gly Cys Ser Thr Gln Ala Gly Ala Phe Asn           
                405                 410                 415               

gaa gaa gtc atc aaa aca atg tat aaa cat aat cca aga cca atg att       1296
Glu Glu Val Ile Lys Thr Met Tyr Lys His Asn Pro Arg Pro Met Ile           
            420                 425                 430                   

ttc cca ttg tcc aac cca act aga tta cat gag tgt ttc cct gaa gac       1344
Phe Pro Leu Ser Asn Pro Thr Arg Leu His Glu Cys Phe Pro Glu Asp           
        435                 440                 445                       

gca ctt aaa tgg acc gat ttc aac gct tta gtt gcc act ggt tct cct       1392
Ala Leu Lys Trp Thr Asp Phe Asn Ala Leu Val Ala Thr Gly Ser Pro           
    450                 455                 460                           

ttc cca cct gtt gaa ggt cat gtt att tct gaa aat aac aat tgt ttt       1440
Phe Pro Pro Val Glu Gly His Val Ile Ser Glu Asn Asn Asn Cys Phe           
465                 470                 475                 480           

gcc ttc ccg ggt att ggt cta ggt gca gtg ctc gct aga act act agg       1488
Ala Phe Pro Gly Ile Gly Leu Gly Ala Val Leu Ala Arg Thr Thr Arg           
                485                 490                 495               

ata tca gac aac atg att tcg gct gcc gtt gac gag cta gct tct ctt       1536
Ile Ser Asp Asn Met Ile Ser Ala Ala Val Asp Glu Leu Ala Ser Leu           
            500                 505                 510                   

tct cca gct caa aaa gat cct aaa ttg ggc ctt tta cct cca att gag       1584
Ser Pro Ala Gln Lys Asp Pro Lys Leu Gly Leu Leu Pro Pro Ile Glu           
        515                 520                 525                       

gaa atc gac gag acc tct gca aga atc gca act gca gtt atc ttg aag       1632
Glu Ile Asp Glu Thr Ser Ala Arg Ile Ala Thr Ala Val Ile Leu Lys           
    530                 535                 540                           

gct gtc gag gaa gga ttt gca aga gta gaa gaa gaa gat tct cca tta       1680
Ala Val Glu Glu Gly Phe Ala Arg Val Glu Glu Glu Asp Ser Pro Leu           
545                 550                 555                 560           

ggt ggt aaa gtt aaa att cca aga gag ttt gat cca tgt cta aga tgg       1728
Gly Gly Lys Val Lys Ile Pro Arg Glu Phe Asp Pro Cys Leu Arg Trp           
                565                 570                 575               

gtt aaa gaa cag atg tgg cat cca att tac aga cca atg atc aaa gtc       1776
Val Lys Glu Gln Met Trp His Pro Ile Tyr Arg Pro Met Ile Lys Val           
            580                 585                 590                   

gca cac tca gac aat att cat act cac caa tac taa                       1812
Ala His Ser Asp Asn Ile His Thr His Gln Tyr                               
        595                 600                                           


<210>  58
<211>  603
<212>  PRT
<213>  Issatchenkia orientalis

<400>  58

Met Thr Lys Asp Cys Cys Glu Val Thr Lys Ile Pro Val Gly Glu Glu 
1               5                   10                  15      


Ala Lys Val Thr Val Pro Arg Ser Thr Arg Leu Ser Ala Thr Gly Pro 
            20                  25                  30          


Val Glu Cys Asp Leu Ser Gly Phe Gln Val Leu Asn Ser Pro Leu Phe 
        35                  40                  45              


Asn Lys Gly Thr Ala Phe Thr Ile Ala Glu Arg Glu Ala Phe Gly Leu 
    50                  55                  60                  


Asn Gly Leu Leu Pro Pro Val Val Asn Thr Leu Glu Glu Gln Val Glu 
65                  70                  75                  80  


Arg Ser Tyr Lys Gln Leu His Phe Leu Lys Thr Pro Leu Ala Lys Asn 
                85                  90                  95      


Asp Phe Cys Thr Ser Leu Arg Leu Gln Asn Lys Val Leu Phe Tyr Arg 
            100                 105                 110         


Leu Val Lys Glu His Ile Lys Glu Leu Ile Pro Ile Val Tyr Thr Pro 
        115                 120                 125             


Thr Glu Gly Asp Ala Ile Ile Ala Tyr Ser Asp Arg Phe Arg Lys Pro 
    130                 135                 140                 


Glu Gly Leu Phe Leu Asp Ile Thr Arg Pro Asn Glu Ile Asp Gln Arg 
145                 150                 155                 160 


Leu Glu Gln Phe Gly Glu Asp Lys Asp Val Asp Tyr Ile Val Ile Thr 
                165                 170                 175     


Asp Ser Glu Gly Ile Leu Gly Ile Gly Asp Gln Gly Val Gly Gly Val 
            180                 185                 190         


Arg Ile Thr Ile Ala Lys Ala Ala Leu Met Thr Val Cys Ala Gly Leu 
        195                 200                 205             


His Pro Gly Arg Val Val Ser Cys Val Leu Asp Val Gly Thr Asn Asn 
    210                 215                 220                 


Val Lys Leu Leu Glu Asp Asp Leu Tyr Leu Gly Asn Arg Phe Pro Arg 
225                 230                 235                 240 


Val Arg Gly Lys Glu Tyr Asp Asp Phe Val Asn Lys Thr Ile Arg Ala 
                245                 250                 255     


Met Lys Lys Arg Phe Pro Ser Ala Val Ile His Phe Glu Asp Phe Gly 
            260                 265                 270         


Val Thr Thr Ala Arg Pro Val Leu Glu Arg Phe Arg Asp Glu Ile Pro 
        275                 280                 285             


Cys Phe Asn Asp Asp Ile Gln Gly Thr Gly Ala Val Val Met Ala Ser 
    290                 295                 300                 


Met Ala Ala Ala Leu Lys Leu Thr His Arg Asn Leu Leu Asp Ser Lys 
305                 310                 315                 320 


Val Leu Ile Tyr Gly Ala Gly Ser Ala Gly Leu Gly Ile Ala Asp Gln 
                325                 330                 335     


Ile Val Asn His Met Val Ser His Gly Ala Thr Lys Glu Glu Ala Arg 
            340                 345                 350         


Arg Lys Ile Tyr Cys Met Asp Arg Tyr Gly Leu Ile Leu Lys Gly Met 
        355                 360                 365             


Thr Ser Asn Ser Pro Ala Gln Glu Asp Tyr Ala His Asp Pro Lys Asp 
    370                 375                 380                 


Trp Glu Asn Ile Ser Thr Thr Ser Leu Val Asp Val Ile Glu Lys Val 
385                 390                 395                 400 


Lys Pro Thr Thr Leu Val Gly Cys Ser Thr Gln Ala Gly Ala Phe Asn 
                405                 410                 415     


Glu Glu Val Ile Lys Thr Met Tyr Lys His Asn Pro Arg Pro Met Ile 
            420                 425                 430         


Phe Pro Leu Ser Asn Pro Thr Arg Leu His Glu Cys Phe Pro Glu Asp 
        435                 440                 445             


Ala Leu Lys Trp Thr Asp Phe Asn Ala Leu Val Ala Thr Gly Ser Pro 
    450                 455                 460                 


Phe Pro Pro Val Glu Gly His Val Ile Ser Glu Asn Asn Asn Cys Phe 
465                 470                 475                 480 


Ala Phe Pro Gly Ile Gly Leu Gly Ala Val Leu Ala Arg Thr Thr Arg 
                485                 490                 495     


Ile Ser Asp Asn Met Ile Ser Ala Ala Val Asp Glu Leu Ala Ser Leu 
            500                 505                 510         


Ser Pro Ala Gln Lys Asp Pro Lys Leu Gly Leu Leu Pro Pro Ile Glu 
        515                 520                 525             


Glu Ile Asp Glu Thr Ser Ala Arg Ile Ala Thr Ala Val Ile Leu Lys 
    530                 535                 540                 


Ala Val Glu Glu Gly Phe Ala Arg Val Glu Glu Glu Asp Ser Pro Leu 
545                 550                 555                 560 


Gly Gly Lys Val Lys Ile Pro Arg Glu Phe Asp Pro Cys Leu Arg Trp 
                565                 570                 575     


Val Lys Glu Gln Met Trp His Pro Ile Tyr Arg Pro Met Ile Lys Val 
            580                 585                 590         


Ala His Ser Asp Asn Ile His Thr His Gln Tyr 
        595                 600             


<210>  59
<211>  1620
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(1620)

<400>  59
atg act tta cac aaa aat tcc aac gaa gga gtt caa gct tca ggg ttt         48
Met Thr Leu His Lys Asn Ser Asn Glu Gly Val Gln Ala Ser Gly Phe           
1               5                   10                  15                

gag ctc caa gat aat ctg gat gtt ccg cat tct aat gca tca ttc caa         96
Glu Leu Gln Asp Asn Leu Asp Val Pro His Ser Asn Ala Ser Phe Gln           
            20                  25                  30                    

agc ttt aaa tcc gac gag gct gaa cag gct cac aat aat gaa cac ttg        144
Ser Phe Lys Ser Asp Glu Ala Glu Gln Ala His Asn Asn Glu His Leu           
        35                  40                  45                        

atg gag aag cct cag ttt aat aag gca aca atc tcc aac tac tgt aaa        192
Met Glu Lys Pro Gln Phe Asn Lys Ala Thr Ile Ser Asn Tyr Cys Lys           
    50                  55                  60                            

aca aga ttt act gag ttg ttc cca aca aag cag tcg atg gct gcc aac        240
Thr Arg Phe Thr Glu Leu Phe Pro Thr Lys Gln Ser Met Ala Ala Asn           
65                  70                  75                  80            

aag cac ctt ttg aat cct ctt cct ggt tta aga atg att ggt ttc aaa        288
Lys His Leu Leu Asn Pro Leu Pro Gly Leu Arg Met Ile Gly Phe Lys           
                85                  90                  95                

caa tgg ctc ttg att tta tct ggg ttc ctt gca tgg act tgg gat gcc        336
Gln Trp Leu Leu Ile Leu Ser Gly Phe Leu Ala Trp Thr Trp Asp Ala           
            100                 105                 110                   

tat gat ttc ttt tcg att tct cta aat act gtc caa ttg gct aag gat        384
Tyr Asp Phe Phe Ser Ile Ser Leu Asn Thr Val Gln Leu Ala Lys Asp           
        115                 120                 125                       

ttt gat aag aca gtc aag gat atc acc tgg ggt att act gtt gtt ttg        432
Phe Asp Lys Thr Val Lys Asp Ile Thr Trp Gly Ile Thr Val Val Leu           
    130                 135                 140                           

atg ttg aga tct gtc ggt ggt ttc ttc ttt ggt tac ttg ggt gat aag        480
Met Leu Arg Ser Val Gly Gly Phe Phe Phe Gly Tyr Leu Gly Asp Lys           
145                 150                 155                 160           

tac ggt aga aaa tgg cct tta att gca aat cta atg tgt gtc tgt ttt        528
Tyr Gly Arg Lys Trp Pro Leu Ile Ala Asn Leu Met Cys Val Cys Phe           
                165                 170                 175               

ctt gaa atc ggt act gga ttt atc aaa aac tac tcc caa ttc ttg ggt        576
Leu Glu Ile Gly Thr Gly Phe Ile Lys Asn Tyr Ser Gln Phe Leu Gly           
            180                 185                 190                   

gtt agg gct gtt ttc ggt att atg ttg ggt ggt gtc tat ggt aat gcg        624
Val Arg Ala Val Phe Gly Ile Met Leu Gly Gly Val Tyr Gly Asn Ala           
        195                 200                 205                       

gct gca act gcg ttg gat gat tgt cct gca gag gca agg ggc ttt att        672
Ala Ala Thr Ala Leu Asp Asp Cys Pro Ala Glu Ala Arg Gly Phe Ile           
    210                 215                 220                           

tct ggt ttc tta caa caa ggt tac gca ttt ggt tat ttg ttg gct gtt        720
Ser Gly Phe Leu Gln Gln Gly Tyr Ala Phe Gly Tyr Leu Leu Ala Val           
225                 230                 235                 240           

gtt ttc aaa aga gca att gct gat aat tcc cct cac aga tgg aga gca        768
Val Phe Lys Arg Ala Ile Ala Asp Asn Ser Pro His Arg Trp Arg Ala           
                245                 250                 255               

atg ttt tgg ttc ggt gct ggt gtc tgt ttc tta att tgt tgc ttc aga        816
Met Phe Trp Phe Gly Ala Gly Val Cys Phe Leu Ile Cys Cys Phe Arg           
            260                 265                 270                   

gct gtc ttg ccg gaa act aag gct ttc caa aga aac aag gaa att gaa        864
Ala Val Leu Pro Glu Thr Lys Ala Phe Gln Arg Asn Lys Glu Ile Glu           
        275                 280                 285                       

agg tat aat gaa gaa cat ggt att cac cag agg tct ttc aag gaa aag        912
Arg Tyr Asn Glu Glu His Gly Ile His Gln Arg Ser Phe Lys Glu Lys           
    290                 295                 300                           

gcc act gct tct ttg aag atc tac tgg ttg atg atc att tac atg gtt        960
Ala Thr Ala Ser Leu Lys Ile Tyr Trp Leu Met Ile Ile Tyr Met Val           
305                 310                 315                 320           

ctg tta atg gct ggt ttc aat ttc atg tcc cat ggt tct caa gat tta       1008
Leu Leu Met Ala Gly Phe Asn Phe Met Ser His Gly Ser Gln Asp Leu           
                325                 330                 335               

tat cca acc tta ttg act gtt aga tat aat ttt agt gat aat gct aca       1056
Tyr Pro Thr Leu Leu Thr Val Arg Tyr Asn Phe Ser Asp Asn Ala Thr           
            340                 345                 350                   

act gtt aca aac tgt gtt gca aat att ggt gca atc aca ggt ggt atc       1104
Thr Val Thr Asn Cys Val Ala Asn Ile Gly Ala Ile Thr Gly Gly Ile           
        355                 360                 365                       

att att gga cat ttc tcg aat ttc att ggt aga aga ttg tcg atc att       1152
Ile Ile Gly His Phe Ser Asn Phe Ile Gly Arg Arg Leu Ser Ile Ile           
    370                 375                 380                           

atc tgc tgt att att ggt ggt gct cta att tat cct tgg gcc ttt gtt       1200
Ile Cys Cys Ile Ile Gly Gly Ala Leu Ile Tyr Pro Trp Ala Phe Val           
385                 390                 395                 400           

gac aat gca aac att aat gca gga gct ttc ttt tta cag ttc ttt gtg       1248
Asp Asn Ala Asn Ile Asn Ala Gly Ala Phe Phe Leu Gln Phe Phe Val           
                405                 410                 415               

caa ggt gct tgg ggt gtt gtt cca gtt cat tta tcc gaa ttg gca cca       1296
Gln Gly Ala Trp Gly Val Val Pro Val His Leu Ser Glu Leu Ala Pro           
            420                 425                 430                   

cct gac ttc aaa gcc ttt gtt gtt ggt att gca tac caa ttg ggt aat       1344
Pro Asp Phe Lys Ala Phe Val Val Gly Ile Ala Tyr Gln Leu Gly Asn           
        435                 440                 445                       

ttg gca tcc tct gca agt tcc acc att gaa aca aca att ggt gtg cac       1392
Leu Ala Ser Ser Ala Ser Ser Thr Ile Glu Thr Thr Ile Gly Val His           
    450                 455                 460                           

ttc cca atg act tct cca ggt ggt gaa cca atc ttt gat tat gca aaa       1440
Phe Pro Met Thr Ser Pro Gly Gly Glu Pro Ile Phe Asp Tyr Ala Lys           
465                 470                 475                 480           

gtt atg gca att ttc gtt ggc tgt gtc ttt gca tat gtg tta ctt atc       1488
Val Met Ala Ile Phe Val Gly Cys Val Phe Ala Tyr Val Leu Leu Ile           
                485                 490                 495               

aca ttt att ggt cca gag agg aaa tct gtg tcc ttt gag gag cca gtt       1536
Thr Phe Ile Gly Pro Glu Arg Lys Ser Val Ser Phe Glu Glu Pro Val           
            500                 505                 510                   

gat gag gat atc gaa atc aat gaa aaa atc aaa cac aat gaa gaa atc       1584
Asp Glu Asp Ile Glu Ile Asn Glu Lys Ile Lys His Asn Glu Glu Ile           
        515                 520                 525                       

gag gct ggc tct aac ttg gga act tca aga gca taa                       1620
Glu Ala Gly Ser Asn Leu Gly Thr Ser Arg Ala                               
    530                 535                                               


<210>  60
<211>  539
<212>  PRT
<213>  Issatchenkia orientalis

<400>  60

Met Thr Leu His Lys Asn Ser Asn Glu Gly Val Gln Ala Ser Gly Phe 
1               5                   10                  15      


Glu Leu Gln Asp Asn Leu Asp Val Pro His Ser Asn Ala Ser Phe Gln 
            20                  25                  30          


Ser Phe Lys Ser Asp Glu Ala Glu Gln Ala His Asn Asn Glu His Leu 
        35                  40                  45              


Met Glu Lys Pro Gln Phe Asn Lys Ala Thr Ile Ser Asn Tyr Cys Lys 
    50                  55                  60                  


Thr Arg Phe Thr Glu Leu Phe Pro Thr Lys Gln Ser Met Ala Ala Asn 
65                  70                  75                  80  


Lys His Leu Leu Asn Pro Leu Pro Gly Leu Arg Met Ile Gly Phe Lys 
                85                  90                  95      


Gln Trp Leu Leu Ile Leu Ser Gly Phe Leu Ala Trp Thr Trp Asp Ala 
            100                 105                 110         


Tyr Asp Phe Phe Ser Ile Ser Leu Asn Thr Val Gln Leu Ala Lys Asp 
        115                 120                 125             


Phe Asp Lys Thr Val Lys Asp Ile Thr Trp Gly Ile Thr Val Val Leu 
    130                 135                 140                 


Met Leu Arg Ser Val Gly Gly Phe Phe Phe Gly Tyr Leu Gly Asp Lys 
145                 150                 155                 160 


Tyr Gly Arg Lys Trp Pro Leu Ile Ala Asn Leu Met Cys Val Cys Phe 
                165                 170                 175     


Leu Glu Ile Gly Thr Gly Phe Ile Lys Asn Tyr Ser Gln Phe Leu Gly 
            180                 185                 190         


Val Arg Ala Val Phe Gly Ile Met Leu Gly Gly Val Tyr Gly Asn Ala 
        195                 200                 205             


Ala Ala Thr Ala Leu Asp Asp Cys Pro Ala Glu Ala Arg Gly Phe Ile 
    210                 215                 220                 


Ser Gly Phe Leu Gln Gln Gly Tyr Ala Phe Gly Tyr Leu Leu Ala Val 
225                 230                 235                 240 


Val Phe Lys Arg Ala Ile Ala Asp Asn Ser Pro His Arg Trp Arg Ala 
                245                 250                 255     


Met Phe Trp Phe Gly Ala Gly Val Cys Phe Leu Ile Cys Cys Phe Arg 
            260                 265                 270         


Ala Val Leu Pro Glu Thr Lys Ala Phe Gln Arg Asn Lys Glu Ile Glu 
        275                 280                 285             


Arg Tyr Asn Glu Glu His Gly Ile His Gln Arg Ser Phe Lys Glu Lys 
    290                 295                 300                 


Ala Thr Ala Ser Leu Lys Ile Tyr Trp Leu Met Ile Ile Tyr Met Val 
305                 310                 315                 320 


Leu Leu Met Ala Gly Phe Asn Phe Met Ser His Gly Ser Gln Asp Leu 
                325                 330                 335     


Tyr Pro Thr Leu Leu Thr Val Arg Tyr Asn Phe Ser Asp Asn Ala Thr 
            340                 345                 350         


Thr Val Thr Asn Cys Val Ala Asn Ile Gly Ala Ile Thr Gly Gly Ile 
        355                 360                 365             


Ile Ile Gly His Phe Ser Asn Phe Ile Gly Arg Arg Leu Ser Ile Ile 
    370                 375                 380                 


Ile Cys Cys Ile Ile Gly Gly Ala Leu Ile Tyr Pro Trp Ala Phe Val 
385                 390                 395                 400 


Asp Asn Ala Asn Ile Asn Ala Gly Ala Phe Phe Leu Gln Phe Phe Val 
                405                 410                 415     


Gln Gly Ala Trp Gly Val Val Pro Val His Leu Ser Glu Leu Ala Pro 
            420                 425                 430         


Pro Asp Phe Lys Ala Phe Val Val Gly Ile Ala Tyr Gln Leu Gly Asn 
        435                 440                 445             


Leu Ala Ser Ser Ala Ser Ser Thr Ile Glu Thr Thr Ile Gly Val His 
    450                 455                 460                 


Phe Pro Met Thr Ser Pro Gly Gly Glu Pro Ile Phe Asp Tyr Ala Lys 
465                 470                 475                 480 


Val Met Ala Ile Phe Val Gly Cys Val Phe Ala Tyr Val Leu Leu Ile 
                485                 490                 495     


Thr Phe Ile Gly Pro Glu Arg Lys Ser Val Ser Phe Glu Glu Pro Val 
            500                 505                 510         


Asp Glu Asp Ile Glu Ile Asn Glu Lys Ile Lys His Asn Glu Glu Ile 
        515                 520                 525             


Glu Ala Gly Ser Asn Leu Gly Thr Ser Arg Ala 
    530                 535                 


<210>  61
<211>  1737
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(1737)

<400>  61
atg tta ctc aga tca cta aac tct tct gct cgt tgt gtc aaa caa aca         48
Met Leu Leu Arg Ser Leu Asn Ser Ser Ala Arg Cys Val Lys Gln Thr           
1               5                   10                  15                

acc aga aca aag gtt agg tat ctc agc cac gtc agt ggt gca agc atg         96
Thr Arg Thr Lys Val Arg Tyr Leu Ser His Val Ser Gly Ala Ser Met           
            20                  25                  30                    

gcg aaa cct aca ttg aag aac aac tcg aga gaa tcc aac aaa tcc aga        144
Ala Lys Pro Thr Leu Lys Asn Asn Ser Arg Glu Ser Asn Lys Ser Arg           
        35                  40                  45                        

aac tat cta att gct gct gtg aca gca ttg gct gta tca acc tca att        192
Asn Tyr Leu Ile Ala Ala Val Thr Ala Leu Ala Val Ser Thr Ser Ile           
    50                  55                  60                            

gga gtt gcc gta cat gtg aag gac ccc ttg tat aac gat gct acc ggc        240
Gly Val Ala Val His Val Lys Asp Pro Leu Tyr Asn Asp Ala Thr Gly           
65                  70                  75                  80            

agt gat tct ccg aga agt ata tct gtt gac gag ttt gtc aag cat aat        288
Ser Asp Ser Pro Arg Ser Ile Ser Val Asp Glu Phe Val Lys His Asn           
                85                  90                  95                

tca caa aac gac tgt tgg att gca atc aat ggc aag gtt tat gat ttc        336
Ser Gln Asn Asp Cys Trp Ile Ala Ile Asn Gly Lys Val Tyr Asp Phe           
            100                 105                 110                   

act gat ttt att cca aac cat cca ggt ggg gta cct cca tta gtt aat        384
Thr Asp Phe Ile Pro Asn His Pro Gly Gly Val Pro Pro Leu Val Asn           
        115                 120                 125                       

cat gct ggt tat gat ggt act aaa ctt tat gag aaa ttg cat cca aaa        432
His Ala Gly Tyr Asp Gly Thr Lys Leu Tyr Glu Lys Leu His Pro Lys           
    130                 135                 140                           

ggt aca att gag aaa ttc ttg cca aag gat aag ttt ctg ggt gtg tta        480
Gly Thr Ile Glu Lys Phe Leu Pro Lys Asp Lys Phe Leu Gly Val Leu           
145                 150                 155                 160           

gat ggt gaa gcg cca aaa ttg gaa gca gac tat ttg gtg gac gat gat        528
Asp Gly Glu Ala Pro Lys Leu Glu Ala Asp Tyr Leu Val Asp Asp Asp           
                165                 170                 175               

gaa caa gag aga ctg gat tat ttg aac aac tta cct cct ttg tca tct        576
Glu Gln Glu Arg Leu Asp Tyr Leu Asn Asn Leu Pro Pro Leu Ser Ser           
            180                 185                 190                   

att cag aat gtt tat gat ttc gaa tac ttg gcc aag aag att tta cct        624
Ile Gln Asn Val Tyr Asp Phe Glu Tyr Leu Ala Lys Lys Ile Leu Pro           
        195                 200                 205                       

aaa gat gcc tgg gca tat tat tct tgt ggt gcc gat gat gaa atc aca        672
Lys Asp Ala Trp Ala Tyr Tyr Ser Cys Gly Ala Asp Asp Glu Ile Thr           
    210                 215                 220                           

atg aga gaa aac cat tat gct tat caa aga gtt tat ttc aga cca aga        720
Met Arg Glu Asn His Tyr Ala Tyr Gln Arg Val Tyr Phe Arg Pro Arg           
225                 230                 235                 240           

att tgt gtt gat gtc aag gaa gtt gat act tct tat gaa atg tta ggc        768
Ile Cys Val Asp Val Lys Glu Val Asp Thr Ser Tyr Glu Met Leu Gly           
                245                 250                 255               

act aaa acc tct gtt cct ttt tat gta tct gcc acc gct ttg gct aaa        816
Thr Lys Thr Ser Val Pro Phe Tyr Val Ser Ala Thr Ala Leu Ala Lys           
            260                 265                 270                   

tta ggc cat cct gat ggt gaa tgc tca att gct aga ggc gct ggt aag        864
Leu Gly His Pro Asp Gly Glu Cys Ser Ile Ala Arg Gly Ala Gly Lys           
        275                 280                 285                       

gaa ggt gtc gtt caa atg att tcg acc ctt tcc tca atg tca tta gat        912
Glu Gly Val Val Gln Met Ile Ser Thr Leu Ser Ser Met Ser Leu Asp           
    290                 295                 300                           

gaa att gcc gct gct aga att cca ggt gca acc caa tgg ttc caa tta        960
Glu Ile Ala Ala Ala Arg Ile Pro Gly Ala Thr Gln Trp Phe Gln Leu           
305                 310                 315                 320           

tac att aat gag gat aga aat gtc gct aaa ggt ctg gtc aaa cat gca       1008
Tyr Ile Asn Glu Asp Arg Asn Val Ala Lys Gly Leu Val Lys His Ala           
                325                 330                 335               

gaa gac ttg ggt atg aag gct atc ttt ata act gtt gat gct cct tct       1056
Glu Asp Leu Gly Met Lys Ala Ile Phe Ile Thr Val Asp Ala Pro Ser           
            340                 345                 350                   

cta ggt aac aga gaa aag gat aaa aga tta aag ttt gtt aat gac acc       1104
Leu Gly Asn Arg Glu Lys Asp Lys Arg Leu Lys Phe Val Asn Asp Thr           
        355                 360                 365                       

gat gtc gat ttg ggt gat tcc gca gat cga aac agt ggt gct tca aag       1152
Asp Val Asp Leu Gly Asp Ser Ala Asp Arg Asn Ser Gly Ala Ser Lys           
    370                 375                 380                           

gca cta tct tcg ttc att gat gct tct gtc tct tgg aat gac gtc aaa       1200
Ala Leu Ser Ser Phe Ile Asp Ala Ser Val Ser Trp Asn Asp Val Lys           
385                 390                 395                 400           

gcg gtc aag tcg tgg act aaa ttg cct gtc tta gtt aaa ggt gtt caa       1248
Ala Val Lys Ser Trp Thr Lys Leu Pro Val Leu Val Lys Gly Val Gln           
                405                 410                 415               

aca gtt gaa gac gtt att gaa gct tac gat gct ggt tgt caa ggt gtt       1296
Thr Val Glu Asp Val Ile Glu Ala Tyr Asp Ala Gly Cys Gln Gly Val           
            420                 425                 430                   

gtt ttg tca aac cac ggt ggt agg caa cta gat act gct cct cct cca       1344
Val Leu Ser Asn His Gly Gly Arg Gln Leu Asp Thr Ala Pro Pro Pro           
        435                 440                 445                       

atc gaa tta tta gct gaa act gtt cca act ttg aag aga ttg ggt aaa       1392
Ile Glu Leu Leu Ala Glu Thr Val Pro Thr Leu Lys Arg Leu Gly Lys           
    450                 455                 460                           

tta aga cca gat ttt gaa att tta att gac ggt ggt gtc aaa aga ggt       1440
Leu Arg Pro Asp Phe Glu Ile Leu Ile Asp Gly Gly Val Lys Arg Gly           
465                 470                 475                 480           

acc gat att ttg aaa gca gtc gca atc ggt ggc caa gat gtc aga gtt       1488
Thr Asp Ile Leu Lys Ala Val Ala Ile Gly Gly Gln Asp Val Arg Val           
                485                 490                 495               

tca gtt ggt atg ggt aga cct ttc tta tat gcc aac tct tgc tat ggt       1536
Ser Val Gly Met Gly Arg Pro Phe Leu Tyr Ala Asn Ser Cys Tyr Gly           
            500                 505                 510                   

gaa gca ggt gtt aga aaa tta att caa aat cta aag gat gaa tta gaa       1584
Glu Ala Gly Val Arg Lys Leu Ile Gln Asn Leu Lys Asp Glu Leu Glu           
        515                 520                 525                       

atg gat atg aga ttg ttg ggt gtc act aaa atg gac cag cta tct tcg       1632
Met Asp Met Arg Leu Leu Gly Val Thr Lys Met Asp Gln Leu Ser Ser           
    530                 535                 540                           

aaa cat gtc gat act aaa cgt ttg att ggt aga gat gcg atc aac tat       1680
Lys His Val Asp Thr Lys Arg Leu Ile Gly Arg Asp Ala Ile Asn Tyr           
545                 550                 555                 560           

ttg tat gat aat gta tac agc cca atc gaa acc gtt aaa ttc aac aat       1728
Leu Tyr Asp Asn Val Tyr Ser Pro Ile Glu Thr Val Lys Phe Asn Asn           
                565                 570                 575               

gaa gat tga                                                           1737
Glu Asp                                                                   
                                                                          


<210>  62
<211>  578
<212>  PRT
<213>  Issatchenkia orientalis

<400>  62

Met Leu Leu Arg Ser Leu Asn Ser Ser Ala Arg Cys Val Lys Gln Thr 
1               5                   10                  15      


Thr Arg Thr Lys Val Arg Tyr Leu Ser His Val Ser Gly Ala Ser Met 
            20                  25                  30          


Ala Lys Pro Thr Leu Lys Asn Asn Ser Arg Glu Ser Asn Lys Ser Arg 
        35                  40                  45              


Asn Tyr Leu Ile Ala Ala Val Thr Ala Leu Ala Val Ser Thr Ser Ile 
    50                  55                  60                  


Gly Val Ala Val His Val Lys Asp Pro Leu Tyr Asn Asp Ala Thr Gly 
65                  70                  75                  80  


Ser Asp Ser Pro Arg Ser Ile Ser Val Asp Glu Phe Val Lys His Asn 
                85                  90                  95      


Ser Gln Asn Asp Cys Trp Ile Ala Ile Asn Gly Lys Val Tyr Asp Phe 
            100                 105                 110         


Thr Asp Phe Ile Pro Asn His Pro Gly Gly Val Pro Pro Leu Val Asn 
        115                 120                 125             


His Ala Gly Tyr Asp Gly Thr Lys Leu Tyr Glu Lys Leu His Pro Lys 
    130                 135                 140                 


Gly Thr Ile Glu Lys Phe Leu Pro Lys Asp Lys Phe Leu Gly Val Leu 
145                 150                 155                 160 


Asp Gly Glu Ala Pro Lys Leu Glu Ala Asp Tyr Leu Val Asp Asp Asp 
                165                 170                 175     


Glu Gln Glu Arg Leu Asp Tyr Leu Asn Asn Leu Pro Pro Leu Ser Ser 
            180                 185                 190         


Ile Gln Asn Val Tyr Asp Phe Glu Tyr Leu Ala Lys Lys Ile Leu Pro 
        195                 200                 205             


Lys Asp Ala Trp Ala Tyr Tyr Ser Cys Gly Ala Asp Asp Glu Ile Thr 
    210                 215                 220                 


Met Arg Glu Asn His Tyr Ala Tyr Gln Arg Val Tyr Phe Arg Pro Arg 
225                 230                 235                 240 


Ile Cys Val Asp Val Lys Glu Val Asp Thr Ser Tyr Glu Met Leu Gly 
                245                 250                 255     


Thr Lys Thr Ser Val Pro Phe Tyr Val Ser Ala Thr Ala Leu Ala Lys 
            260                 265                 270         


Leu Gly His Pro Asp Gly Glu Cys Ser Ile Ala Arg Gly Ala Gly Lys 
        275                 280                 285             


Glu Gly Val Val Gln Met Ile Ser Thr Leu Ser Ser Met Ser Leu Asp 
    290                 295                 300                 


Glu Ile Ala Ala Ala Arg Ile Pro Gly Ala Thr Gln Trp Phe Gln Leu 
305                 310                 315                 320 


Tyr Ile Asn Glu Asp Arg Asn Val Ala Lys Gly Leu Val Lys His Ala 
                325                 330                 335     


Glu Asp Leu Gly Met Lys Ala Ile Phe Ile Thr Val Asp Ala Pro Ser 
            340                 345                 350         


Leu Gly Asn Arg Glu Lys Asp Lys Arg Leu Lys Phe Val Asn Asp Thr 
        355                 360                 365             


Asp Val Asp Leu Gly Asp Ser Ala Asp Arg Asn Ser Gly Ala Ser Lys 
    370                 375                 380                 


Ala Leu Ser Ser Phe Ile Asp Ala Ser Val Ser Trp Asn Asp Val Lys 
385                 390                 395                 400 


Ala Val Lys Ser Trp Thr Lys Leu Pro Val Leu Val Lys Gly Val Gln 
                405                 410                 415     


Thr Val Glu Asp Val Ile Glu Ala Tyr Asp Ala Gly Cys Gln Gly Val 
            420                 425                 430         


Val Leu Ser Asn His Gly Gly Arg Gln Leu Asp Thr Ala Pro Pro Pro 
        435                 440                 445             


Ile Glu Leu Leu Ala Glu Thr Val Pro Thr Leu Lys Arg Leu Gly Lys 
    450                 455                 460                 


Leu Arg Pro Asp Phe Glu Ile Leu Ile Asp Gly Gly Val Lys Arg Gly 
465                 470                 475                 480 


Thr Asp Ile Leu Lys Ala Val Ala Ile Gly Gly Gln Asp Val Arg Val 
                485                 490                 495     


Ser Val Gly Met Gly Arg Pro Phe Leu Tyr Ala Asn Ser Cys Tyr Gly 
            500                 505                 510         


Glu Ala Gly Val Arg Lys Leu Ile Gln Asn Leu Lys Asp Glu Leu Glu 
        515                 520                 525             


Met Asp Met Arg Leu Leu Gly Val Thr Lys Met Asp Gln Leu Ser Ser 
    530                 535                 540                 


Lys His Val Asp Thr Lys Arg Leu Ile Gly Arg Asp Ala Ile Asn Tyr 
545                 550                 555                 560 


Leu Tyr Asp Asn Val Tyr Ser Pro Ile Glu Thr Val Lys Phe Asn Asn 
                565                 570                 575     


Glu Asp 
        


<210>  63
<211>  1698
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(1698)

<220>
<221>  misc_feature
<222>  (941)..(941)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (943)..(943)
<223>  n is a, c, g, or t

<400>  63
atg tta aga tcc cag ttc aaa aac att ttg aaa aat gtt aac aag aac         48
Met Leu Arg Ser Gln Phe Lys Asn Ile Leu Lys Asn Val Asn Lys Asn           
1               5                   10                  15                

cat tct cta agg aga act ttt act tcc agc acc tca aag gct gga aaa         96
His Ser Leu Arg Arg Thr Phe Thr Ser Ser Thr Ser Lys Ala Gly Lys           
            20                  25                  30                    

aat gct tca tac aat gcc aag att ata tct gca acc gtg gcc tcg att        144
Asn Ala Ser Tyr Asn Ala Lys Ile Ile Ser Ala Thr Val Ala Ser Ile           
        35                  40                  45                        

gtt gca gca gct ggc tct tat atg ttg gtc cag cct tca cta gct aat        192
Val Ala Ala Ala Gly Ser Tyr Met Leu Val Gln Pro Ser Leu Ala Asn           
    50                  55                  60                            

gat gag gca cag tct gct aat cca act agg aag atc tct gtt gac gaa        240
Asp Glu Ala Gln Ser Ala Asn Pro Thr Arg Lys Ile Ser Val Asp Glu           
65                  70                  75                  80            

ttt gtt aaa cac aac cat gcc gat gat tgt tgg atc act gtt aac ggt        288
Phe Val Lys His Asn His Ala Asp Asp Cys Trp Ile Thr Val Asn Gly           
                85                  90                  95                

aac gtc tat gac ttg act gat ttc att tca atg cat cca ggt ggt act        336
Asn Val Tyr Asp Leu Thr Asp Phe Ile Ser Met His Pro Gly Gly Thr           
            100                 105                 110                   

acc cca ttg att caa aat gca ggt cac gac gca act gaa att tac aac        384
Thr Pro Leu Ile Gln Asn Ala Gly His Asp Ala Thr Glu Ile Tyr Asn           
        115                 120                 125                       

aag att cat cca aag ggt aca atc gag aac ttc tta cca aag gaa aag        432
Lys Ile His Pro Lys Gly Thr Ile Glu Asn Phe Leu Pro Lys Glu Lys           
    130                 135                 140                           

caa ttg ggt gtt ttg gat ggt gaa gct cct aaa atc gaa gtt gtg ctt        480
Gln Leu Gly Val Leu Asp Gly Glu Ala Pro Lys Ile Glu Val Val Leu           
145                 150                 155                 160           

gac gaa aag gag aaa cac aga ttg gag ttg ttg aat cat ctc cct gct        528
Asp Glu Lys Glu Lys His Arg Leu Glu Leu Leu Asn His Leu Pro Ala           
                165                 170                 175               

ctt tcc aga att caa aac att tat gat ttc gaa cat att gct tct aga        576
Leu Ser Arg Ile Gln Asn Ile Tyr Asp Phe Glu His Ile Ala Ser Arg           
            180                 185                 190                   

gtt ttg agc gac caa gca tgg aac tac tat tca tgt ggt gcc gaa gat        624
Val Leu Ser Asp Gln Ala Trp Asn Tyr Tyr Ser Cys Gly Ala Glu Asp           
        195                 200                 205                       

gaa atc acc ttg agg gaa aat cat tat gct tac caa aga atc tac ttt        672
Glu Ile Thr Leu Arg Glu Asn His Tyr Ala Tyr Gln Arg Ile Tyr Phe           
    210                 215                 220                           

aag cca aaa tgt tgt gtc aat gtt gca gaa gtt gat acc tct cat gaa        720
Lys Pro Lys Cys Cys Val Asn Val Ala Glu Val Asp Thr Ser His Glu           
225                 230                 235                 240           

att tta ggt aca aaa gct tct gtt cct ttc tac gtt tcc gca gcc gct        768
Ile Leu Gly Thr Lys Ala Ser Val Pro Phe Tyr Val Ser Ala Ala Ala           
                245                 250                 255               

tct gca aag ttg ggg cac gag gat ggt gaa tgt tcc att gct aga ggt        816
Ser Ala Lys Leu Gly His Glu Asp Gly Glu Cys Ser Ile Ala Arg Gly           
            260                 265                 270                   

gca ggt aag gaa ggc gtt att caa atg att tct tcc ttc tct tcc aac        864
Ala Gly Lys Glu Gly Val Ile Gln Met Ile Ser Ser Phe Ser Ser Asn           
        275                 280                 285                       

tct ttg gag gaa att gca gaa tcc aga att cct ggt gca aca caa tgg        912
Ser Leu Glu Glu Ile Ala Glu Ser Arg Ile Pro Gly Ala Thr Gln Trp           
    290                 295                 300                           

ttt caa tta tac gtt aat gaa gac aag gnt ntt gtg aag aag act tta        960
Phe Gln Leu Tyr Val Asn Glu Asp Lys Xaa Xaa Val Lys Lys Thr Leu           
305                 310                 315                 320           

aaa agg gcc gaa aac ttg ggt atg aag gcc atc ttt gtc act gtg gac       1008
Lys Arg Ala Glu Asn Leu Gly Met Lys Ala Ile Phe Val Thr Val Asp           
                325                 330                 335               

gct gct agt aga ggt aat aga gaa aaa gac att aca atg aga att acc       1056
Ala Ala Ser Arg Gly Asn Arg Glu Lys Asp Ile Thr Met Arg Ile Thr           
            340                 345                 350                   

gaa gat aca gat gag tta ata gac gat tct tct gtt aga gct ggt tct       1104
Glu Asp Thr Asp Glu Leu Ile Asp Asp Ser Ser Val Arg Ala Gly Ser           
        355                 360                 365                       

acc tct ggt gca ttg cca gct ttc att gac aag agg ctg act tgg gat       1152
Thr Ser Gly Ala Leu Pro Ala Phe Ile Asp Lys Arg Leu Thr Trp Asp           
    370                 375                 380                           

gaa gtt aag gat atc att tca tgg acc aag tta cca gtt ttg ctg aag       1200
Glu Val Lys Asp Ile Ile Ser Trp Thr Lys Leu Pro Val Leu Leu Lys           
385                 390                 395                 400           

ggt gtt caa aga act gat gat att gag aag gca att gat att ggt tgt       1248
Gly Val Gln Arg Thr Asp Asp Ile Glu Lys Ala Ile Asp Ile Gly Cys           
                405                 410                 415               

aag ggt gtt gtc ttg tcc aat cat ggt ggt aga caa tta gat act tct       1296
Lys Gly Val Val Leu Ser Asn His Gly Gly Arg Gln Leu Asp Thr Ser           
            420                 425                 430                   

cct cct cca ata gaa gtt atg gct gaa tct gtt cca atc cta aag caa       1344
Pro Pro Pro Ile Glu Val Met Ala Glu Ser Val Pro Ile Leu Lys Gln           
        435                 440                 445                       

aag ggt aaa ctg gat cca aat ttc agt att ttc gtt gat ggt ggt gtt       1392
Lys Gly Lys Leu Asp Pro Asn Phe Ser Ile Phe Val Asp Gly Gly Val           
    450                 455                 460                           

aga aga ggt aca gat att ttg aaa gct ttg gct att ggt ggc aga gac       1440
Arg Arg Gly Thr Asp Ile Leu Lys Ala Leu Ala Ile Gly Gly Arg Asp           
465                 470                 475                 480           

tgt aaa gtt gct gtt ggt ctg ggt aga cct ttc ctt tat gca aat act       1488
Cys Lys Val Ala Val Gly Leu Gly Arg Pro Phe Leu Tyr Ala Asn Thr           
                485                 490                 495               

ggt tat ggt gaa aag ggt gtc aga aag gcc gtg caa att cta aga gaa       1536
Gly Tyr Gly Glu Lys Gly Val Arg Lys Ala Val Gln Ile Leu Arg Glu           
            500                 505                 510                   

gaa tta aag gct gac atg aga atg ttg ggc gtt acc tct ttg aac gag       1584
Glu Leu Lys Ala Asp Met Arg Met Leu Gly Val Thr Ser Leu Asn Glu           
        515                 520                 525                       

cta gac gac tct tac att gac acc aga aga tta cta ggt aga gat gct       1632
Leu Asp Asp Ser Tyr Ile Asp Thr Arg Arg Leu Leu Gly Arg Asp Ala           
    530                 535                 540                           

gtt aac cac ata tac aac aac aac tac tac cca atg tct aag att caa       1680
Val Asn His Ile Tyr Asn Asn Asn Tyr Tyr Pro Met Ser Lys Ile Gln           
545                 550                 555                 560           

ttc aaa aac gaa aaa taa                                               1698
Phe Lys Asn Glu Lys                                                       
                565                                                       


<210>  64
<211>  565
<212>  PRT
<213>  Issatchenkia orientalis

<220>
<221>  misc_feature
<222>  (314)..(314)
<223>  The 'Xaa' at location 314 stands for Asp, Gly, Ala, or Val.

<220>
<221>  misc_feature
<222>  (315)..(315)
<223>  The 'Xaa' at location 315 stands for Ile, Val, Leu, or Phe.

<400>  64

Met Leu Arg Ser Gln Phe Lys Asn Ile Leu Lys Asn Val Asn Lys Asn 
1               5                   10                  15      


His Ser Leu Arg Arg Thr Phe Thr Ser Ser Thr Ser Lys Ala Gly Lys 
            20                  25                  30          


Asn Ala Ser Tyr Asn Ala Lys Ile Ile Ser Ala Thr Val Ala Ser Ile 
        35                  40                  45              


Val Ala Ala Ala Gly Ser Tyr Met Leu Val Gln Pro Ser Leu Ala Asn 
    50                  55                  60                  


Asp Glu Ala Gln Ser Ala Asn Pro Thr Arg Lys Ile Ser Val Asp Glu 
65                  70                  75                  80  


Phe Val Lys His Asn His Ala Asp Asp Cys Trp Ile Thr Val Asn Gly 
                85                  90                  95      


Asn Val Tyr Asp Leu Thr Asp Phe Ile Ser Met His Pro Gly Gly Thr 
            100                 105                 110         


Thr Pro Leu Ile Gln Asn Ala Gly His Asp Ala Thr Glu Ile Tyr Asn 
        115                 120                 125             


Lys Ile His Pro Lys Gly Thr Ile Glu Asn Phe Leu Pro Lys Glu Lys 
    130                 135                 140                 


Gln Leu Gly Val Leu Asp Gly Glu Ala Pro Lys Ile Glu Val Val Leu 
145                 150                 155                 160 


Asp Glu Lys Glu Lys His Arg Leu Glu Leu Leu Asn His Leu Pro Ala 
                165                 170                 175     


Leu Ser Arg Ile Gln Asn Ile Tyr Asp Phe Glu His Ile Ala Ser Arg 
            180                 185                 190         


Val Leu Ser Asp Gln Ala Trp Asn Tyr Tyr Ser Cys Gly Ala Glu Asp 
        195                 200                 205             


Glu Ile Thr Leu Arg Glu Asn His Tyr Ala Tyr Gln Arg Ile Tyr Phe 
    210                 215                 220                 


Lys Pro Lys Cys Cys Val Asn Val Ala Glu Val Asp Thr Ser His Glu 
225                 230                 235                 240 


Ile Leu Gly Thr Lys Ala Ser Val Pro Phe Tyr Val Ser Ala Ala Ala 
                245                 250                 255     


Ser Ala Lys Leu Gly His Glu Asp Gly Glu Cys Ser Ile Ala Arg Gly 
            260                 265                 270         


Ala Gly Lys Glu Gly Val Ile Gln Met Ile Ser Ser Phe Ser Ser Asn 
        275                 280                 285             


Ser Leu Glu Glu Ile Ala Glu Ser Arg Ile Pro Gly Ala Thr Gln Trp 
    290                 295                 300                 


Phe Gln Leu Tyr Val Asn Glu Asp Lys Xaa Xaa Val Lys Lys Thr Leu 
305                 310                 315                 320 


Lys Arg Ala Glu Asn Leu Gly Met Lys Ala Ile Phe Val Thr Val Asp 
                325                 330                 335     


Ala Ala Ser Arg Gly Asn Arg Glu Lys Asp Ile Thr Met Arg Ile Thr 
            340                 345                 350         


Glu Asp Thr Asp Glu Leu Ile Asp Asp Ser Ser Val Arg Ala Gly Ser 
        355                 360                 365             


Thr Ser Gly Ala Leu Pro Ala Phe Ile Asp Lys Arg Leu Thr Trp Asp 
    370                 375                 380                 


Glu Val Lys Asp Ile Ile Ser Trp Thr Lys Leu Pro Val Leu Leu Lys 
385                 390                 395                 400 


Gly Val Gln Arg Thr Asp Asp Ile Glu Lys Ala Ile Asp Ile Gly Cys 
                405                 410                 415     


Lys Gly Val Val Leu Ser Asn His Gly Gly Arg Gln Leu Asp Thr Ser 
            420                 425                 430         


Pro Pro Pro Ile Glu Val Met Ala Glu Ser Val Pro Ile Leu Lys Gln 
        435                 440                 445             


Lys Gly Lys Leu Asp Pro Asn Phe Ser Ile Phe Val Asp Gly Gly Val 
    450                 455                 460                 


Arg Arg Gly Thr Asp Ile Leu Lys Ala Leu Ala Ile Gly Gly Arg Asp 
465                 470                 475                 480 


Cys Lys Val Ala Val Gly Leu Gly Arg Pro Phe Leu Tyr Ala Asn Thr 
                485                 490                 495     


Gly Tyr Gly Glu Lys Gly Val Arg Lys Ala Val Gln Ile Leu Arg Glu 
            500                 505                 510         


Glu Leu Lys Ala Asp Met Arg Met Leu Gly Val Thr Ser Leu Asn Glu 
        515                 520                 525             


Leu Asp Asp Ser Tyr Ile Asp Thr Arg Arg Leu Leu Gly Arg Asp Ala 
    530                 535                 540                 


Val Asn His Ile Tyr Asn Asn Asn Tyr Tyr Pro Met Ser Lys Ile Gln 
545                 550                 555                 560 


Phe Lys Asn Glu Lys 
                565 


<210>  65
<211>  933
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(933)

<400>  65
atg act ttg aag tcc aaa tca aaa tcc aaa aag aag cac gca tcc tca         48
Met Thr Leu Lys Ser Lys Ser Lys Ser Lys Lys Lys His Ala Ser Ser           
1               5                   10                  15                

aaa cca ctg gaa tca tct aat aaa atg tcc aaa agt agt gtt gaa cac         96
Lys Pro Leu Glu Ser Ser Asn Lys Met Ser Lys Ser Ser Val Glu His           
            20                  25                  30                    

cat gaa cat acc tca aat aag gaa aat gac cat att tcc ttg cat tcc        144
His Glu His Thr Ser Asn Lys Glu Asn Asp His Ile Ser Leu His Ser           
        35                  40                  45                        

cgc ttg acg aat att gaa cat cag atc atg ggc aaa gta cat aca agt        192
Arg Leu Thr Asn Ile Glu His Gln Ile Met Gly Lys Val His Thr Ser           
    50                  55                  60                            

gat gac ggc gcc tat gtt atc tta gac aac aaa aag tat cct aag tca        240
Asp Asp Gly Ala Tyr Val Ile Leu Asp Asn Lys Lys Tyr Pro Lys Ser           
65                  70                  75                  80            

gaa tta ttg aag gcc ttt ggt ggt ttt atg aac cct ggt tgg gca gtg        288
Glu Leu Leu Lys Ala Phe Gly Gly Phe Met Asn Pro Gly Trp Ala Val           
                85                  90                  95                

cct tcc gaa cac aag ttt ggt aat cca gct cct ttg ggt cta tct gca        336
Pro Ser Glu His Lys Phe Gly Asn Pro Ala Pro Leu Gly Leu Ser Ala           
            100                 105                 110                   

ttt gcg tat tgt act ttt gtt gca tct ttg gtc aac atg caa act aga        384
Phe Ala Tyr Cys Thr Phe Val Ala Ser Leu Val Asn Met Gln Thr Arg           
        115                 120                 125                       

cat gtt gaa aat gat gct gtt aat gtc ggt gct gca atg ttt tat ggt        432
His Val Glu Asn Asp Ala Val Asn Val Gly Ala Ala Met Phe Tyr Gly           
    130                 135                 140                           

ggt ttt atc cag ttc att gcc gga ctt tgg gaa ata tcg ctt gaa aac        480
Gly Phe Ile Gln Phe Ile Ala Gly Leu Trp Glu Ile Ser Leu Glu Asn           
145                 150                 155                 160           

gct ttt ggt ggt ttg gca ttt tgc tct ttt gga ggt tac tgg atg gca        528
Ala Phe Gly Gly Leu Ala Phe Cys Ser Phe Gly Gly Tyr Trp Met Ala           
                165                 170                 175               

tcg gcc tca aac cat atc ccc tgg ttc cat att gct agc tct tat act        576
Ser Ala Ser Asn His Ile Pro Trp Phe His Ile Ala Ser Ser Tyr Thr           
            180                 185                 190                   

aca gaa gca gaa ttc aaa tca ggt atg gga ttt ttc tac ctt ggt tgg        624
Thr Glu Ala Glu Phe Lys Ser Gly Met Gly Phe Phe Tyr Leu Gly Trp           
        195                 200                 205                       

cta ctc ttt aca ata atc ttg cta gct tgt tca atc aaa tct acc att        672
Leu Leu Phe Thr Ile Ile Leu Leu Ala Cys Ser Ile Lys Ser Thr Ile           
    210                 215                 220                           

tta ttt ttc ctg ttg ttt gtg ctg gtc ttt atg aga ttg ctg tta tta        720
Leu Phe Phe Leu Leu Phe Val Leu Val Phe Met Arg Leu Leu Leu Leu           
225                 230                 235                 240           

aca tgt tgg aag ttt gcg gac agt cat gcc tgt gag ttt gct gct ggt        768
Thr Cys Trp Lys Phe Ala Asp Ser His Ala Cys Glu Phe Ala Ala Gly           
                245                 250                 255               

gtt ttc ggt gtt ttg gca tct ctg tta gca tgg tat cat gca tat gca        816
Val Phe Gly Val Leu Ala Ser Leu Leu Ala Trp Tyr His Ala Tyr Ala           
            260                 265                 270                   

ggt att gca aca cct cag aat tct tac tat gtt gtt aat cca aca cct        864
Gly Ile Ala Thr Pro Gln Asn Ser Tyr Tyr Val Val Asn Pro Thr Pro           
        275                 280                 285                       

atg cct gtt att gga tca aag agc aaa gat atg ttt gat tct gac gac        912
Met Pro Val Ile Gly Ser Lys Ser Lys Asp Met Phe Asp Ser Asp Asp           
    290                 295                 300                           

ttt gac caa tct tca tct tga                                            933
Phe Asp Gln Ser Ser Ser                                                   
305                 310                                                   


<210>  66
<211>  310
<212>  PRT
<213>  Issatchenkia orientalis

<400>  66

Met Thr Leu Lys Ser Lys Ser Lys Ser Lys Lys Lys His Ala Ser Ser 
1               5                   10                  15      


Lys Pro Leu Glu Ser Ser Asn Lys Met Ser Lys Ser Ser Val Glu His 
            20                  25                  30          


His Glu His Thr Ser Asn Lys Glu Asn Asp His Ile Ser Leu His Ser 
        35                  40                  45              


Arg Leu Thr Asn Ile Glu His Gln Ile Met Gly Lys Val His Thr Ser 
    50                  55                  60                  


Asp Asp Gly Ala Tyr Val Ile Leu Asp Asn Lys Lys Tyr Pro Lys Ser 
65                  70                  75                  80  


Glu Leu Leu Lys Ala Phe Gly Gly Phe Met Asn Pro Gly Trp Ala Val 
                85                  90                  95      


Pro Ser Glu His Lys Phe Gly Asn Pro Ala Pro Leu Gly Leu Ser Ala 
            100                 105                 110         


Phe Ala Tyr Cys Thr Phe Val Ala Ser Leu Val Asn Met Gln Thr Arg 
        115                 120                 125             


His Val Glu Asn Asp Ala Val Asn Val Gly Ala Ala Met Phe Tyr Gly 
    130                 135                 140                 


Gly Phe Ile Gln Phe Ile Ala Gly Leu Trp Glu Ile Ser Leu Glu Asn 
145                 150                 155                 160 


Ala Phe Gly Gly Leu Ala Phe Cys Ser Phe Gly Gly Tyr Trp Met Ala 
                165                 170                 175     


Ser Ala Ser Asn His Ile Pro Trp Phe His Ile Ala Ser Ser Tyr Thr 
            180                 185                 190         


Thr Glu Ala Glu Phe Lys Ser Gly Met Gly Phe Phe Tyr Leu Gly Trp 
        195                 200                 205             


Leu Leu Phe Thr Ile Ile Leu Leu Ala Cys Ser Ile Lys Ser Thr Ile 
    210                 215                 220                 


Leu Phe Phe Leu Leu Phe Val Leu Val Phe Met Arg Leu Leu Leu Leu 
225                 230                 235                 240 


Thr Cys Trp Lys Phe Ala Asp Ser His Ala Cys Glu Phe Ala Ala Gly 
                245                 250                 255     


Val Phe Gly Val Leu Ala Ser Leu Leu Ala Trp Tyr His Ala Tyr Ala 
            260                 265                 270         


Gly Ile Ala Thr Pro Gln Asn Ser Tyr Tyr Val Val Asn Pro Thr Pro 
        275                 280                 285             


Met Pro Val Ile Gly Ser Lys Ser Lys Asp Met Phe Asp Ser Asp Asp 
    290                 295                 300                 


Phe Asp Gln Ser Ser Ser 
305                 310 


<210>  67
<211>  1134
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(1134)

<400>  67
atg tta tcc aag acc atc act gct gca ttg agg ggc aat aca act cgt         48
Met Leu Ser Lys Thr Ile Thr Ala Ala Leu Arg Gly Asn Thr Thr Arg           
1               5                   10                  15                

act gca ttc aga atc aat gcc att aga agt tta gcg atc cca gct att         96
Thr Ala Phe Arg Ile Asn Ala Ile Arg Ser Leu Ala Ile Pro Ala Ile           
            20                  25                  30                    

cca gag aca caa aag ggt gtt atc ttt tat gag aac gga ggt gaa cta        144
Pro Glu Thr Gln Lys Gly Val Ile Phe Tyr Glu Asn Gly Gly Glu Leu           
        35                  40                  45                        

ttt tac aag gac att cca gtt cca aag cca aag cca aat gag att ttg        192
Phe Tyr Lys Asp Ile Pro Val Pro Lys Pro Lys Pro Asn Glu Ile Leu           
    50                  55                  60                            

gtg aat gtc aag tat tct ggt gtt tgt cat acc gat tta cac gca tgg        240
Val Asn Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu His Ala Trp           
65                  70                  75                  80            

aaa ggt gac tgg cct ttg gcg acc aag ttg cca ttg gtt ggt gga cat        288
Lys Gly Asp Trp Pro Leu Ala Thr Lys Leu Pro Leu Val Gly Gly His           
                85                  90                  95                

gaa ggt gcc gga gtt gtt gtt gct aag ggg gac aat gtc acc aac ttt        336
Glu Gly Ala Gly Val Val Val Ala Lys Gly Asp Asn Val Thr Asn Phe           
            100                 105                 110                   

gaa att ggc gat tat gcc ggt atc aag tgg ttg aat ggt tca tgt atg        384
Glu Ile Gly Asp Tyr Ala Gly Ile Lys Trp Leu Asn Gly Ser Cys Met           
        115                 120                 125                       

ggg tgt gaa ttt tgc caa caa ggt gca gag cca aac tgt cca cag gcc        432
Gly Cys Glu Phe Cys Gln Gln Gly Ala Glu Pro Asn Cys Pro Gln Ala           
    130                 135                 140                           

gac ttg agt ggt tac acc cat gac ggg tcc ttt caa caa tat gcc act        480
Asp Leu Ser Gly Tyr Thr His Asp Gly Ser Phe Gln Gln Tyr Ala Thr           
145                 150                 155                 160           

gcc gat gct gtt cag gca gcc aag att cct cag ggc act gat ttg gct        528
Ala Asp Ala Val Gln Ala Ala Lys Ile Pro Gln Gly Thr Asp Leu Ala           
                165                 170                 175               

caa gtt gcg cca att tta tgt gca ggt att act gtc tat aag gct tta        576
Gln Val Ala Pro Ile Leu Cys Ala Gly Ile Thr Val Tyr Lys Ala Leu           
            180                 185                 190                   

aag act gca gaa tta aga cca ggt caa tgg gtt gcc att tct ggt gct        624
Lys Thr Ala Glu Leu Arg Pro Gly Gln Trp Val Ala Ile Ser Gly Ala           
        195                 200                 205                       

gct gga ggt tta ggt tct ctt gct gtt caa tat gcc aag gcc atg ggt        672
Ala Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Lys Ala Met Gly           
    210                 215                 220                           

ttg aga gtt ttg ggt att gat ggt ggt gag gag aag ggc aag ttt gca        720
Leu Arg Val Leu Gly Ile Asp Gly Gly Glu Glu Lys Gly Lys Phe Ala           
225                 230                 235                 240           

aag tct ctt gga gct gaa gtt ttc att gat ttc acc aaa tcc aag gac        768
Lys Ser Leu Gly Ala Glu Val Phe Ile Asp Phe Thr Lys Ser Lys Asp           
                245                 250                 255               

att gtc aag gat atc caa gag gcc acc aat ggt ggt cca cat ggt gtc        816
Ile Val Lys Asp Ile Gln Glu Ala Thr Asn Gly Gly Pro His Gly Val           
            260                 265                 270                   

att aat gtt tct gtt tct cca gct gct att tct caa agt acc cag tat        864
Ile Asn Val Ser Val Ser Pro Ala Ala Ile Ser Gln Ser Thr Gln Tyr           
        275                 280                 285                       

gtc aga acc ttg ggt aag gtt gtc ctt gtt gga tta cca gcg cat gct        912
Val Arg Thr Leu Gly Lys Val Val Leu Val Gly Leu Pro Ala His Ala           
    290                 295                 300                           

gta tgc gag tct tcg gtt ttc gac cat gtt gtc aag tcg att caa att        960
Val Cys Glu Ser Ser Val Phe Asp His Val Val Lys Ser Ile Gln Ile           
305                 310                 315                 320           

aga ggc tct tat gtt ggt aac agg gaa gat act agt gag gct att gat       1008
Arg Gly Ser Tyr Val Gly Asn Arg Glu Asp Thr Ser Glu Ala Ile Asp           
                325                 330                 335               

ttt ttc acc agg ggt tta gtg aag tca cca att aag att gtt ggt ttg       1056
Phe Phe Thr Arg Gly Leu Val Lys Ser Pro Ile Lys Ile Val Gly Leu           
            340                 345                 350                   

agt gag ttg cca aag atc tat gaa ttg atg gag caa ggt aag att tta       1104
Ser Glu Leu Pro Lys Ile Tyr Glu Leu Met Glu Gln Gly Lys Ile Leu           
        355                 360                 365                       

ggc aga tat gtt gtt gac act tcg aaa tga                               1134
Gly Arg Tyr Val Val Asp Thr Ser Lys                                       
    370                 375                                               


<210>  68
<211>  377
<212>  PRT
<213>  Issatchenkia orientalis

<400>  68

Met Leu Ser Lys Thr Ile Thr Ala Ala Leu Arg Gly Asn Thr Thr Arg 
1               5                   10                  15      


Thr Ala Phe Arg Ile Asn Ala Ile Arg Ser Leu Ala Ile Pro Ala Ile 
            20                  25                  30          


Pro Glu Thr Gln Lys Gly Val Ile Phe Tyr Glu Asn Gly Gly Glu Leu 
        35                  40                  45              


Phe Tyr Lys Asp Ile Pro Val Pro Lys Pro Lys Pro Asn Glu Ile Leu 
    50                  55                  60                  


Val Asn Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu His Ala Trp 
65                  70                  75                  80  


Lys Gly Asp Trp Pro Leu Ala Thr Lys Leu Pro Leu Val Gly Gly His 
                85                  90                  95      


Glu Gly Ala Gly Val Val Val Ala Lys Gly Asp Asn Val Thr Asn Phe 
            100                 105                 110         


Glu Ile Gly Asp Tyr Ala Gly Ile Lys Trp Leu Asn Gly Ser Cys Met 
        115                 120                 125             


Gly Cys Glu Phe Cys Gln Gln Gly Ala Glu Pro Asn Cys Pro Gln Ala 
    130                 135                 140                 


Asp Leu Ser Gly Tyr Thr His Asp Gly Ser Phe Gln Gln Tyr Ala Thr 
145                 150                 155                 160 


Ala Asp Ala Val Gln Ala Ala Lys Ile Pro Gln Gly Thr Asp Leu Ala 
                165                 170                 175     


Gln Val Ala Pro Ile Leu Cys Ala Gly Ile Thr Val Tyr Lys Ala Leu 
            180                 185                 190         


Lys Thr Ala Glu Leu Arg Pro Gly Gln Trp Val Ala Ile Ser Gly Ala 
        195                 200                 205             


Ala Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Lys Ala Met Gly 
    210                 215                 220                 


Leu Arg Val Leu Gly Ile Asp Gly Gly Glu Glu Lys Gly Lys Phe Ala 
225                 230                 235                 240 


Lys Ser Leu Gly Ala Glu Val Phe Ile Asp Phe Thr Lys Ser Lys Asp 
                245                 250                 255     


Ile Val Lys Asp Ile Gln Glu Ala Thr Asn Gly Gly Pro His Gly Val 
            260                 265                 270         


Ile Asn Val Ser Val Ser Pro Ala Ala Ile Ser Gln Ser Thr Gln Tyr 
        275                 280                 285             


Val Arg Thr Leu Gly Lys Val Val Leu Val Gly Leu Pro Ala His Ala 
    290                 295                 300                 


Val Cys Glu Ser Ser Val Phe Asp His Val Val Lys Ser Ile Gln Ile 
305                 310                 315                 320 


Arg Gly Ser Tyr Val Gly Asn Arg Glu Asp Thr Ser Glu Ala Ile Asp 
                325                 330                 335     


Phe Phe Thr Arg Gly Leu Val Lys Ser Pro Ile Lys Ile Val Gly Leu 
            340                 345                 350         


Ser Glu Leu Pro Lys Ile Tyr Glu Leu Met Glu Gln Gly Lys Ile Leu 
        355                 360                 365             


Gly Arg Tyr Val Val Asp Thr Ser Lys 
    370                 375         


<210>  69
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  69
tctctggtct tcaaacatgg                                                   20


<210>  70
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  70
cttcaaagtg gtgcatgcgg tgag                                              24


<210>  71
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  71
actcttctgc tcgttgtgtc                                                   20


<210>  72
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  72
gtttgaccag acctttagcg                                                   20


<210>  73
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  73
caacggcaac agtttacagg                                                   20


<210>  74
<211>  1032
<212>  DNA
<213>  Bacteriophage P1


<220>
<221>  CDS
<222>  (1)..(1032)

<400>  74
atg tct aat tta ctt act gtt cac caa aac ttg cct gca tta cca gtt         48
Met Ser Asn Leu Leu Thr Val His Gln Asn Leu Pro Ala Leu Pro Val           
1               5                   10                  15                

gac gca acc tcc gat gaa gtc aga aag aac ctt atg gat atg ttt aga         96
Asp Ala Thr Ser Asp Glu Val Arg Lys Asn Leu Met Asp Met Phe Arg           
            20                  25                  30                    

gat aga caa gct ttc tcc gaa cat act tgg aaa atg tta tta tcc gtt        144
Asp Arg Gln Ala Phe Ser Glu His Thr Trp Lys Met Leu Leu Ser Val           
        35                  40                  45                        

tgt aga tcc tgg gcc gct tgg tgt aaa ctt aac aat aga aaa tgg ttt        192
Cys Arg Ser Trp Ala Ala Trp Cys Lys Leu Asn Asn Arg Lys Trp Phe           
    50                  55                  60                            

cct gct gaa cca gaa gac gtc aga gat tac tta ctt tac tta caa gct        240
Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu Tyr Leu Gln Ala           
65                  70                  75                  80            

aga ggt ttg gct gtt aaa act atc caa caa cac tta ggt caa ttg aat        288
Arg Gly Leu Ala Val Lys Thr Ile Gln Gln His Leu Gly Gln Leu Asn           
                85                  90                  95                

atg tta cac aga aga tcc ggt tta cca aga cca tcc gat tcc aac gca        336
Met Leu His Arg Arg Ser Gly Leu Pro Arg Pro Ser Asp Ser Asn Ala           
            100                 105                 110                   

gtt tcc ctt gtt atg aga aga att aga aaa gaa aat gtt gac gct ggt        384
Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly           
        115                 120                 125                       

gaa aga gct aaa caa gca tta gca ttt gaa aga acc gat ttc gat caa        432
Glu Arg Ala Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln           
    130                 135                 140                           

gtt aga tcc tta atg gaa aat tcc gat aga tgt caa gat att aga aac        480
Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn           
145                 150                 155                 160           

tta gct ttc tta ggt att gct tac aac aca tta tta aga atc gct gaa        528
Leu Ala Phe Leu Gly Ile Ala Tyr Asn Thr Leu Leu Arg Ile Ala Glu           
                165                 170                 175               

att gct aga att aga gtt aaa gat att tca aga acc gat ggc ggt aga        576
Ile Ala Arg Ile Arg Val Lys Asp Ile Ser Arg Thr Asp Gly Gly Arg           
            180                 185                 190                   

atg tta atc cac att ggc aga aca aaa acc tta gtc tcc aca gca ggc        624
Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Thr Ala Gly           
        195                 200                 205                       

gtc gaa aaa gca tta tca tta ggt gtt act aaa tta gtt gaa cgt tgg        672
Val Glu Lys Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp           
    210                 215                 220                           

att tcc gtt tcc ggt gtt gca gat gac cca aac aac tac tta ttc tgt        720
Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys           
225                 230                 235                 240           

cgt gtt aga aaa aat ggt gtt gcc gct cct tcc gct acc tca caa tta        768
Arg Val Arg Lys Asn Gly Val Ala Ala Pro Ser Ala Thr Ser Gln Leu           
                245                 250                 255               

tcc aca aga gca tta gaa ggc att ttt gaa gct acc cac aga ctt att        816
Ser Thr Arg Ala Leu Glu Gly Ile Phe Glu Ala Thr His Arg Leu Ile           
            260                 265                 270                   

tat ggt gca aaa gac gat tcc ggt caa aga tat tta gct tgg tct ggt        864
Tyr Gly Ala Lys Asp Asp Ser Gly Gln Arg Tyr Leu Ala Trp Ser Gly           
        275                 280                 285                       

cat tcc gct aga gtt ggt gcc gca aga gac atg gca aga gct ggt gtt        912
His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val           
    290                 295                 300                           

tct att cct gaa att atg caa gcc ggt ggt tgg act aat gtt aac att        960
Ser Ile Pro Glu Ile Met Gln Ala Gly Gly Trp Thr Asn Val Asn Ile           
305                 310                 315                 320           

gtt atg aac tat atc aga aac tta gat tcc gaa aca ggt gct atg gtt       1008
Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val           
                325                 330                 335               

aga tta ctt gaa gac ggt gat taa                                       1032
Arg Leu Leu Glu Asp Gly Asp                                               
            340                                                           


<210>  75
<211>  343
<212>  PRT
<213>  Bacteriophage P1

<400>  75

Met Ser Asn Leu Leu Thr Val His Gln Asn Leu Pro Ala Leu Pro Val 
1               5                   10                  15      


Asp Ala Thr Ser Asp Glu Val Arg Lys Asn Leu Met Asp Met Phe Arg 
            20                  25                  30          


Asp Arg Gln Ala Phe Ser Glu His Thr Trp Lys Met Leu Leu Ser Val 
        35                  40                  45              


Cys Arg Ser Trp Ala Ala Trp Cys Lys Leu Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  


Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu Tyr Leu Gln Ala 
65                  70                  75                  80  


Arg Gly Leu Ala Val Lys Thr Ile Gln Gln His Leu Gly Gln Leu Asn 
                85                  90                  95      


Met Leu His Arg Arg Ser Gly Leu Pro Arg Pro Ser Asp Ser Asn Ala 
            100                 105                 110         


Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly 
        115                 120                 125             


Glu Arg Ala Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 


Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 


Leu Ala Phe Leu Gly Ile Ala Tyr Asn Thr Leu Leu Arg Ile Ala Glu 
                165                 170                 175     


Ile Ala Arg Ile Arg Val Lys Asp Ile Ser Arg Thr Asp Gly Gly Arg 
            180                 185                 190         


Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Thr Ala Gly 
        195                 200                 205             


Val Glu Lys Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 


Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 


Arg Val Arg Lys Asn Gly Val Ala Ala Pro Ser Ala Thr Ser Gln Leu 
                245                 250                 255     


Ser Thr Arg Ala Leu Glu Gly Ile Phe Glu Ala Thr His Arg Leu Ile 
            260                 265                 270         


Tyr Gly Ala Lys Asp Asp Ser Gly Gln Arg Tyr Leu Ala Trp Ser Gly 
        275                 280                 285             


His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 


Ser Ile Pro Glu Ile Met Gln Ala Gly Gly Trp Thr Asn Val Asn Ile 
305                 310                 315                 320 


Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     


Arg Leu Leu Glu Asp Gly Asp 
            340             


<210>  76
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  76
gaaaggatcc atgtctaatt tacttactgt tcac                                   34


<210>  77
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  77
gaaattaatt aacttaatca ccgtcttcaa gtaatctaac c                           41


<210>  78
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  78
gaaaatgcat gcaacggcaa catcaatgtc cacg                                   34


<210>  79
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  79
gaaagtcgac ggtaaggccc gggaattcag cttgc                                  35


<210>  80
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  80
aaaaaaacgc gtatgtcaac tgtggaagat cac                                    33


<210>  81
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  81
aaaaaacctg caggttaagc tgctggcgct tcatctttgg                             40


<210>  82
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  82
cagagagagg aagaagttgg aac                                               23


<210>  83
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  83
gtacagagaa cttgtaaaca attc                                              24


<210>  84
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  84
taatgggtac accatacggt ggattcccag ag                                     32


<210>  85
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  85
ctctgggaat ccaccgtatg gtgtacccat ta                                     32


<210>  86
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  86
aatattctat tattatatat tttcttccca ataaaacaaa ataaaacaaa acacagcaaa       60

acacaaaaat cctggaattc gcccttacat atgg                                   94


<210>  87
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  87
tatagattgt aaagtagacg taaagtttag taattcattt taatgttcat tttacattca       60

gatgtcatta cggctcgtgc tatattcttg                                        90


<210>  88
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  88
attctattat tatatatttt cttcccaata aaacaaaata aaacaaaaca cagcaaaaca       60

caaaaatcct ggaattcgcc cttacatatg                                        90


<210>  89
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  89
gcgcatccat attttggcgg                                                   20


<210>  90
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  90
ggagtgacac aacctgaaag                                                   20


<210>  91
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  91
aaaggctgac ggacacaatc                                                   20


<210>  92
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  92
atattagtcg acccttctat cagggaaggg ag                                     32


<210>  93
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  93
ttggcgcttc accatctaac                                                   20


<210>  94
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  94
ggtgcttcaa aggcactatc                                                   20


<210>  95
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  95
gtgggctaca aatgatacga tgg                                               23


<210>  96
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  96
tcggccactt gtttattggg                                                   20


<210>  97
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  97
ctgcaggttt gccagcttac                                                   20


<210>  98
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  98
gattgtgtat tagtgtattt cg                                                22


<210>  99
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  99
gaaattgccg ctgctagaat cccaggtgca acccaatggt tc                          42


<210>  100
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  100
gaaccattgg gttgcacctg ggattctagc agcggcaatt tc                          42


<210>  101
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  101
gtccaggagt ccatcggttc ctgtcagatg gg                                     32


<210>  102
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  102
gcgtctagaa tttttgtgtt ttgctgtgtt ttgttttatt ttg                         43


<210>  103
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  103
ggtaccgcgg ccgcggatcc ctcgaggcct taattaacat ctgaatgtaa aatgaacatt       60

aaaatgaatt ac                                                           72


<210>  104
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  104
gagctcgcgg ccgcggattc gccgaatcct tttattataa aattatatat tattcttaat       60

tacatatcac                                                              70


<210>  105
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  105
gatgaacgaa ggtaccgagc tctaagtagt ggtgttggtg aactc                       45


<210>  106
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  106
attatggtag cggccgcatt tggcaaggcg tatctatata ggagg                       45


<210>  107
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  107
ttgccaaatg cggccgctac cataatgtat gcgttgagcc tcttg                       45


<210>  108
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  108
ggttcaatgg gccctaaaag tgttggtgta ttagatgagt ttgtcc                      46


<210>  109
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  109
ttaattaaca agggcgattt ctgcagatat cggccg                                 36


<210>  110
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  110
cggccgatat ctgcagaaat cgcccttgtt aattaa                                 36


<210>  111
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  111
ggaggaatgg aacagtgatg ac                                                22


<210>  112
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  112
caagagtatc ccatctgaca ggaaccgatg g                                      31


<210>  113
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  113
cttctatagg ttgagaccc                                                    19


<210>  114
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  114
catcactgtt aaaggaatgg gtaaatc                                           27


<210>  115
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  115
gcatctaatg tacgttccaa c                                                 21


<210>  116
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  116
cgaaaccgtt aaattcaaca atg                                               23


<210>  117
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  117
gtttaaacgt acgactcatg gggctttaca aac                                    33


<210>  118
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  118
gagctcagcg gccgctcata tgaagtgttg cagttgtttt cttcttaata aag              53


<210>  119
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  119
catatgagcg gccgctgagc tctctagagt ccctctaatt cttcctgttg                  50


<210>  120
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  120
gtttaaacca gtgatcgagc actcatttat acgagatg                               38


<210>  121
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  121
aaaaaaacgc gtatgcctca ttctatcaac g                                      31


<210>  122
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  122
aaaaaacctg caggttacaa actcaaaccg tttctg                                 36


<210>  123
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  123
caaaacaact gtcccctatg tacatc                                            26


<210>  124
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  124
ctgaagttga acaaatttat gccacgcagc ttttc                                  35


<210>  125
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  125
gagtgacaca acctgaaag                                                    19


<210>  126
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  126
gtacagagaa cttgtaaaca attc                                              24


<210>  127
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  127
catgcatgca tgtctagata aaatgttagc tgctagatc                              39


<210>  128
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  128
catgcatgca tgttaattaa cttaatcctt tggaccaatc atg                         43


<210>  129
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  129
gagaacttat acgcaccaga acgccttttg                                        30


<210>  130
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  130
gaggaagttc aaagtatgaa agacgtcag                                         29


<210>  131
<211>  180
<212>  DNA
<213>  Issatchenkia orientalis

<400>  131
gtatctgttg ttgttttatt tcctttggaa gtgtataaaa caaacctagt ccccgctttt       60

gtttttctct cacaaccctt tagagtaagg attagcttgg tatctatttt ttattttcgt      120

tgaaacaagt ttagtcaggt gcttgaaaca caaccaaaca agtaataagt ttgacataga      180


<210>  132
<211>  180
<212>  DNA
<213>  Issatchenkia orientalis

<400>  132
ttgtttccct tcgctttaac tcctatcaat aatacattca aaacttagta ttactgatgc       60

tgttacgact actaatacta ttgctactac caatactatt cacttctcta tctattttag      120

atatatatgc atatagtcat tttttctttt ttttttttga tatctatact ctacactata      180


<210>  133
<211>  379
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RIOR43690 upstream/downstream construct

<400>  133
gtatctgttg ttgttttatt tcctttggaa gtgtataaaa caaacctagt ccccgctttt       60

gtttttctct cacaaccctt tagagtaagg attagcttgg tatctatttt ttattttcgt      120

tgaaacaagt ttagtcaggt gcttgaaaca caaccaaaca agtaataagt ttgacataga      180

catatgaaaa aaagagctct tgtttccctt cgctttaact cctatcaata atacattcaa      240

aacttagtat tactgatgct gttacgacta ctaatactat tgctactacc aatactattc      300

acttctctat ctattttaga tatatatgca tatagtcatt ttttcttttt tttttttgat      360

atctatactc tacactata                                                   379


<210>  134
<211>  1138
<212>  DNA
<213>  Schizosaccharomyces pombe

<400>  134
cggtttgaac accattggca agattgttta catcttacaa atctttttgt tttctctttt       60

tggctcttgt atgttgtttc gtttcatcaa gtatccatct accattaagg actcttggaa      120

tcatcacttg gaaaagttgt ttatcgcaac ttgtttgtta tctatttcca cattcatcga      180

catgttagct atctatgctt atccagatac cggtgaatgg atggtctggg tcattagaat      240

cttatactac atctatgtcg ctgtctcttt catctactgt gttatggcct ttttcaccat      300

tttcaacaat catgtttaca ctattgaaac tgcttctcca gcttggattt tgccaatctt      360

ccctccaatg atctgtggtg tcattgctgg tgctgttaac tccacccaac ctgctcacca      420

attgaaaaac atggtcattt tcggtatctt gtttcaaggt ttaggttttt gggtttacct      480

tttacttttc gccgttaatg ttttgagatt cttcacagtc ggtttagcaa agccacaaga      540

tagaccaggt atgtttatgt tcgttggtcc accagctttc tctggtttag cattgattaa      600

cattgcaaga ggtgcaatgg gctcaagacc ttacattttc gttggtgcaa actcttccga      660

atacttaggt tttgtctcaa ccttcatggc cattttcatc tggggtttag ccgcatggtg      720

ttattgctta gctatggttt ccttccttgc cggctttttc actagagcac cattgaaatt      780

cgcttgtggt tggttcgctt tcatctttcc aaatgttggt tttgttaact gtactatcga      840

aatcggcaag atgattgatt ctaaggcttt tcaaatgttt ggtcacatca ttggtgttat      900

cttgtgtatt caatggattt tgttaatgta cttaatggtt agagcattcc ttgttaatga      960

cttgtgctat cctggtaaag acgaagatgc acacccacca ccaaagccaa acactggtgt     1020

cttaaaccca actttcccac cagagaaggc tccagcatca ttagagaagg ttgatactca     1080

tgttacatca acaggtggtg aatccgatcc tccatcttcc gaacatgaat ccgtttaa       1138


<210>  135
<211>  400
<212>  DNA
<213>  Schizosaccharomyces pombe

<400>  135
aaacgcgtat gggtgaattg aaagagattt tgaaacaaag atatcatgaa ttacttgatt       60

ggaatgttaa ggcaccacat gtccctttat cccagagatt gaagcacttt acttggtcat      120

ggtttgcttg tactatggca accggtggtg ttggtttgat cattggttcc ttcccattca      180

gattctacgg tttgaacacc attggcaaga ttgtttacat cttacaaatc tttttgtttt      240

ctctttttgg ctcttgtatg ttgtttcgtt tcatcaagta tccatctacc attaaggact      300

cttggaatca tcacttggaa aagttgttta tcgcaacttg tttgttatct atttccacat      360

tcatcgacat gttagctatc tatgctcctg caggaatcct                            400


<210>  136
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  136
gaagtcatat ttcgacactc                                                   20


<210>  137
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  137
catcaatagg taccgagctc ccgtttcgat gggattccca gaag                        44


<210>  138
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  138
cccctggtgc ggccgctccc ttctctaaat ggactgcttg g                           41


<210>  139
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  139
agaagggagc ggccgcacca ggggtttagt gaagtcacc                              39


<210>  140
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  140
acttacaagg gccccataac tgacatttat ggtaaggttg ctc                         43


<210>  141
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  141
caggatcgaa gaatagaagt tgtgtg                                            26


<210>  142
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  142
gaacgtctac aacgaggtga acac                                              24


<210>  143
<211>  4910
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  URA3 gene disruption fragment


<220>
<221>  misc_feature
<222>  (1656)..(1656)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (4312)..(4312)
<223>  n is a, c, g, or t

<400>  143
ctcaaaacta tttaattagt taattgtata aactgtatgt cattataaac agggaaggtt       60

gacattgtct agcggcaatc attgtctcat ttggttcatt aactttggtt ctgttcttgg      120

aaacgggtac caactctctc agagtgcttc aaaaattttt cagcacattt ggttagacat      180

gaactttctc tgctggttaa ggattcagag gtgaagtctt gaacacaatc gttgaaacat      240

ctgtccacaa gagatgtgta tagcctcatg aaatcagcca tttgcttttg ttcaacgatc      300

ttttgaaatt gttgttgttc ttggtagtta agttgatcca tcttggctta tgttgtgtgt      360

atgttgtagt tattcttagt atattcctgt cctgagttta gtgaaacata atatcgcctt      420

gaaatgaaaa tgctgaaatt cgtcgacata caatttttca aacttttttt ttttcttggt      480

gcacggacat gtttttaaag gaagtactct ataccagtta ttcttcaccc tgcagggtac      540

gtagcatgca ctcgcaagct gtgccatcgc ccaacggtta attataagaa atcaacatca      600

gccaacaact attttcgtcc ccctcttttc agtggtaacg agcaattaca ttagtaagag      660

actattttct tcagtgattt gtaatttttt ttcagtgatt tgtaattctt tctcgaaata      720

tgcgggctta acttatccgg acattcacta catgcaagga aaaacgagaa ccgcggagat      780

ttcctcagta agtaacaatg atgatctttt tacgcttcat catcactttc caaagttcta      840

agctataagt tcaagcctag atacgctgaa aaactcctga ccaacaatgt aaagaaaaca      900

attacaattg taaggttgaa aacatctaaa aatgaaatat tttattgtac atgcacaccc      960

tgatagtcat tctcttactt catccctgaa agacgtggct gtacaagagt tggaatcgca     1020

aggtcatgag gttaaagtta gtgatcttta tgctcaaaag tggaaggcct caatagaccg     1080

tgacgacttc gagcagcttt tcgcaagaag agaggttaaa aataccccaa gcttcttatg     1140

aagcgtatgc cagaggagca ttaacaaaag acgtaaatca ggaacaggaa aaacttattt     1200

gggcggactt tgtcattttg tcgtttccta tatggtggtc ttctatgccg gctagtcgac     1260

cccctcgacc ccctcgagcg atctcgagat ttgctgcaac ggcaacatca atgtccacgt     1320

ttacacacct acatttatat ctatatttat atttatattt atttatttat gctacttagc     1380

ttctatagtt agttaatgca ctcacgatat tcaaaattga cacccttcaa ctactcccta     1440

ctattgtcta ctactgtcta ctactcctct ttactatagc tgctcccaat aggctccacc     1500

aataggctct gtcaatacat tttgcgccgc cacctttcag gttgtgtcac tcctgaagga     1560

ccatattggg taatcgtgca atttctggaa gagagtgccg cgagaagtga ggcccccact     1620

gtaaatcctc gagggggcat ggagtatggg gcatgnagga tggaggatgg gggggggggg     1680

ggaaaatagg tagcgaaagg acccgctatc accccacccg gagaactcgt tgccgggaag     1740

tcatatttcg acactccggg gagtctataa aaggcgggtt ttgtcttttg ccagttgatg     1800

ttgctgagag gacttgtttg ccgtttcttc cgatttaaca gtatagaatc aaccactgtt     1860

aattatacac gttatactaa cacaacaaaa acaaaaacaa cgacaacaac aacaacaatg     1920

tttgctttct actttctcac cgcatgcacc actttgaagg gtgttttcgg agtttctccg     1980

agttacaatg gtcttggtct caccccacag atgggttggg acagctggaa tacgtttgcc     2040

tgcgatgtca gtgaacagct acttctagac actgctgata gaatttctga cttggggcta     2100

aaggatatgg gttacaagta tgtcatccta gatgactgtt ggtctagcgg cagggattcc     2160

gacggtttcc tcgttgcaga caagcacaaa tttcccaacg gtatgggcca tgttgcagac     2220

cacctgcata ataacagctt tcttttcggt atgtattcgt ctgctggtga gtacacctgt     2280

gctgggtacc ctgggtctct ggggcgtgag gaagaagatg ctcaattctt tgcaaataac     2340

cgcgttgact acttgaagta tgataattgt tacaataaag gtcaatttgg tacaccagac     2400

gtttcttacc accgttacaa ggccatgtca gatgctttga ataaaactgg taggcctatt     2460

ttctattctc tatgtaactg gggtcaggat ttgacatttt actggggctc tggtatcgcc     2520

aattcttgga gaatgagcgg agatattact gctgagttca cccgtccaga tagcagatgt     2580

ccctgtgacg gtgacgaata tgattgcaag tacgccggtt tccattgttc tattatgaat     2640

attcttaaca aggcagctcc aatggggcaa aatgcaggtg ttggtggttg gaacgatctg     2700

gacaatctag aggtcggagt cggtaatttg actgacgatg aggaaaaggc ccatttctct     2760

atgtgggcaa tggtaaagtc cccacttatc attggtgccg acgtgaatca cttaaaggca     2820

tcttcgtact cgatctacag tcaagcctct gtcatcgcaa ttaatcaaga tccaaagggt     2880

attccagcca caagagtctg gagatattat gtttcagaca ccgatgaata tggacaaggt     2940

gaaattcaaa tgtggagtgg tccgcttgac aatggtgacc aagtggttgc tttattgaat     3000

ggaggaagcg tagcaagacc aatgaacacg accttggaag agattttctt tgacagcaat     3060

ttgggttcaa aggaactgac atcgacttgg gatatttacg acttatgggc caacagagtt     3120

gacaactcta cggcgtctgc tatccttgaa cagaataagg cagccaccgg tattctctac     3180

aatgctacag agcagtctta taaagacggt ttgtctaaga atgatacaag actgtttggc     3240

cagaaaattg gtagtctttc tccaaatgct atacttaaca caactgttcc agctcatggt     3300

atcgccttct ataggttgag accctcggct taagctcaat gttgagcaaa gcaggacgag     3360

aaaaaaaaaa ataatgattg ttaagaagtt catgaaaaaa aaaaggaaaa atactcaaat     3420

acttataaca gagtgattaa ataataaacg gcagtatacc ctatcaggta ttgagatagt     3480

tttatttttg taggtatata atctgaagcc tttgaactat tttctcgtat atatcatgga     3540

gtatacattg cattagcaac attgcatact agtcactcgc aagctgtgcc atcgcccaac     3600

ggttaattat aagaaatcaa catcagccaa caactatttt cgtccccctc ttttcagtgg     3660

taacgagcaa ttacattagt aagagactat tttcttcagt gatttgtaat tttttttcag     3720

tgatttgtaa ttctttctcg aaatatgcgg gctwaamtaa tccggacatt cactacatgc     3780

aaggaaaaac gagaaccgcg gagatttcct cagtaagtaa caatgatgat ctttttacgc     3840

ttcatcatca ctttccaaag ttctaagcta taagttcaag cctagatacg ctgaaaaact     3900

cctgaccaac aatgtaaaga aaacaattac aattgtaagg ttgaaaacat ctaaaaatga     3960

aatattttat tgtacatgca caccctgata gtcattctct tacttcatcc ctgaaagacg     4020

tggctgtaca agagttggaa tcgcaaggtc atgaggttaa agttagtgat ctttatgctc     4080

aaaagtggaa ggcctcaata gaccgtgacg acwwmaaaaa amaaamrmaa gaagagaggt     4140

taaaaatacc ccaagcttct tatgaagcgt atgccagagg agcattaaca aaagacgtaa     4200

atcaggaaca ggaaaaactt atttgggcgg actttgtcat tttgtcgttt cctatatggt     4260

ggtcttctat gccggctagc ggccgggcaa caaagcctcc cagatttgat anattttcaa     4320

tttgtgcttt gaatcatgac ttccacctgt ttggtccgca agaacacgta aatgcgcaat     4380

ttgtttctcc cttctgctta aaaaccatgc acctttaata ttatctggaa agataaagaa     4440

cagaattgtt gcgtagaaac aagtagcaga gccgtaaatg agaaaaatat acttccaagc     4500

tggtaatttc ccctttatta gtccaataca gtgtccgaag accccaccaa gaataccagc     4560

aagggtgttg aaatataatg tagatcttag tggttgttct gatttcttcc accacattcc     4620

gctaataatc ataaaagacg gtaatattcc ggcttcaaat acgccaagaa aaaacctcac     4680

ggtaaccaaa ccaccaaagc tatgacatgc agccatgcac ataagtaagc cgccccaaat     4740

gaacaaacaa atagacacaa atttgccaat tctaactcgt ggcaacaaaa aaaaggatat     4800

gaactcacct aataaataac cgaaataaaa agtagaagca actgtggaaa attgagaacc     4860

atgtaaattt gtgtcttctt tcaatgtata aacagccgca atacctaggg                4910


<210>  144
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  144
gcaactgatg ttcacgaatg cg                                                22


<210>  145
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  145
ttgccgttgc agcaaatctc                                                   20


<210>  146
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  146
acggcagtat accctatcag g                                                 21


<210>  147
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  147
aatgatccat ggtccgagag                                                   20


<210>  148
<211>  3704
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDC gene disruption fragment

<400>  148
cccccagttg ttgttgcaat taacaaattt gctaccgaca ccgagaagga aattgagacc       60

attagagaag aagccatcaa ggctggtgca tttgatgctg ttgagtcaga ccattggtca      120

caaggtggta agggtgcaat caagttagct gaggcaattg tacgtgctac cgaggaaaga      180

ccgttggaag aaagtcaacc tcctaactat ctttattcat tagatggttc gttagaagat      240

agactaagaa caattgccac caagatgtat ggagcaaaag atattgaact atctgagttg      300

gccaagaaac agattgaaga gtatgagagt caaggttttg gcaagctgcc tgtttgtatt      360

gcaaagacgc aatattctct ctcccatgat ccaacattga aaggtgttcc aaaggatttc      420

atcttcccaa tcagagaagt tagaataagt gcaggagcag gatatttata tgcactagct      480

gcaaagatca tgacaattcc aggtctatca acttatgccg gatttatgaa tgttgaagtc      540

aacgaagatg gtgagattga tggattgttt tagtttttat tataaaatta tatattattc      600

ttaattacat atcacccttc tatcagggaa gggagaaacg aaaatagaga gtgacctatc      660

caagctcggg ggtctaagtt ttaatggccc agggaatcat tacttttttt tctcaatcct      720

tgatggataa aagtattaca tacgtacagg attgtgtatt agtgtatttc gttatatgat      780

taaacaaagt ttatagattg taaagtagac gtaaagttta gtaattcatt ttaatgttca      840

ttttacattc agatggcggc cgcggatcca gatcccccgg ggcgttgaag atctattctc      900

cagcaattaa atttgtgaag aataactggt atagagtact tcctttaaaa acatgtccgt      960

gcaccaagaa aaaaaaaaag tttgaaaaat tgtatgtcga cgaatttcag cattttcatt     1020

tcaaggcgat attatgtttc actaaactca ggacaggaat atactaagaa taactacaac     1080

atacacacaa cataagccaa gatggatcaa cttaactacc aagaacaaca acaatttcaa     1140

aagatcgttg aacaaaagca aatggctgat ttcatgaggc tatctgcaga tacgcggaac     1200

aatcaatcga taatgatttg actgataaag aaaaccatac ttttgtttat gtttattagt     1260

tatcgctttg ctacattaaa aattcacata ctaaagcctt tgttaaacaa ctttttctaa     1320

atcttaagat tttactctat ctagtttttt tggttgtagg tgaacgtaaa gtacctcatt     1380

tatttttttt tttttgcttg tgtaattctt ttcatgctta tttaaactag tgtacatgta     1440

tcaaatcttt gtgtaagaat catttaaatc tgtttaaata agcattccaa ccagcttgtt     1500

ggtatctttt agcttgctct ataggatctc ttccttgacc gtacaaacct ctaccaacaa     1560

ttatgatatc cgttccagtc tttacaactt catcaacagt tctatattgt tgaccaagtg     1620

catcaccttt gtcatctaaa ccaacccctg gagtcataat gatccagtca aaaccttctt     1680

ctctaccgcc catatcgtgt tgcgcaataa aaccaatgac aaactcttta tcagatttag     1740

caatttctac tgttttttct gtatattcac catatgctaa agaacccttt gatgataact     1800

cagcaagcat tagcaaacct ctaggttcac tggttgtttc ttgggctgcc tccttcaagc     1860

cagaaacaat acctgcaccc gttacaccat gtgcattagt gatgtcagcc cattcggcaa     1920

tacggaagac accagattta tattgatttt taacagtgtt accaatatca gcaaattttc     1980

tatcttcaaa aatcataaaa ttatgtttct tggcaagctc cttcaaaggc aacacagttc     2040

cttcatacgt aaaatcagaa acaatatcga tgtgtgtttt aactagacag atgtaaggac     2100

caatagtgtc caaaatagag agaagctttt cagtttcagt aatatccaat gatgcacaaa     2160

ggttagactt cttttcctcc atgatggaga aaagtctcct agcaacaggg gaagtgtgtg     2220

attctgatct ttctttgtat gacgccatcc ttgacaaaca aactacttta ttaaagcgtt     2280

gaagatctat tctccagcaa ttaaatttgt gaagaataac tggtatagag tacttccttt     2340

aaaaacatgt ccgtgcacca agaaaaaaaa aaagtttgaa aaattgtatg tcgacgaatt     2400

tcagcatttt catttcaagg cgatattatg tttcactaaa ctcaggacag gaatatacta     2460

agaataacta caacatacac acaacataag ccaagatgga tcaacttaac taccaagaac     2520

aacaacaatt tcaaaagatc gttgaacaaa agcaaatggc tgatttcatg aggctatgaa     2580

ttcttttatt ataaaattat atattattct taattacata tcacccttct atcagggaag     2640

ggagaaacga aaatagagag tgacctatcc aagcttgggg gtctaagttt taatggccca     2700

gggaatcatt actttttttt ctcaatcctt gatggataaa agtattacat acgtacagga     2760

ttgtgtatta gtgtatttcg ttatatgatt aaacaaagtt tatagattgt aaagtagacg     2820

taaagtttag taattcattt taatgttcat tttacattca gatgttaatt aaggcctcga     2880

gggatccgcg gccgctattt ttgtgttttg ctgtgttttg ttttattttg ttttattggg     2940

aagaaaatat ataataatag aatattatat taacaaataa ttaaagaagc tcaactgtta     3000

ttagaataaa tgggttctcc gtgtcctttt tatacgcctt ctccgaaaag aaaaaaacca     3060

tcgtatcatt tgtagcccac gccacccgga aaaaccacca ttgtcctcag cagtccgcaa     3120

aaatatggat gcgctcaatc aatttccctc ccccgtcaat gccaaaagga taacgacaca     3180

ctattaagag cgcatcattt gtaaaagccg aggaaggggg atacgctgac cgagacgtct     3240

cgcctcactc tcggagctga gccgccctcc ttaagaaatt catgggaaga acacccttcg     3300

cggcttctga acggctcgcc ctcgtccatt ggtcacctca cagtggcaac taataaggac     3360

attatagcaa tagaaattaa aatggtgcac agaaatacaa taggatcgaa taggatagga     3420

tacaataaga tacggaatat tagactatac tgtgatacgg tacgctacga tacgctacga     3480

tacgatacga tagaggatac cacggatata acgtagtgtt atttttcatt attggggttt     3540

tttttctgtt tgaattttcc acgtcaagag tatcccatct gacaggaacc gatggactcg     3600

tcacagtacc tatcgcccga gttcaatcca tggacgctgc gggtgaagga tcttcgcccg     3660

ctgttggcaa gccatgggat cagggcgtcg ccaagggacg ggcc                      3704


<210>  149
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  149
gaagagacgt acaagatccg cc                                                22


<210>  150
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  150
ggataaaagt attacatacg tacaggattg tgtattagtg tatttcg                     47


<210>  151
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  151
taggaatggt gcatcatcca ac                                                22


<210>  152
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  152
gctggagaat agatcttcaa cgccccg                                           27


<210>  153
<211>  6392
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PYC gene integration fragment

<400>  153
ctaagtagtg gtgttggtga actcaagatg gactctttag gtaattatat tcttgaatag       60

ttgtgtaaag cgaatatgca aatagatttg ttttataatt atgcatctct ttgaaagagg      120

tttagaggca aagttcttgc atacaatatt gtgattgttt taatgtcatt cttgattttc      180

ataaagagat taaaaaaaaa aaaaaaaaac ttataaaatt gagtagaacc atttatatat      240

aagacaaaga ttgtctgtat tagtcctcaa cacactaaac cttacatact tagggtaaat      300

ttgctaatag agtgatatgt tcatgagaac tccaacgaca acacaaccac ctatttgcac      360

aacaaacacc attgtcgcac gctgcgcgcc ctagaagtag aaagaaaggg aaatgacatt      420

aagagaatca taccccgtgc ccgtaacgcc gaaaaaatca caccccgtcc cccacacctt      480

aaaacctcaa ccgcttaaca ccgccacacc ctttctcttt ataaacgccg tttgcattac      540

tcattcttct tataaaccgc accccccaaa acgcggaata gcttcaaccc cccaatcaga      600

tatgagtttc ccgggaaacc cgcttttccc gacagcccca caaggggttg gtctataaaa      660

gaggacgttt tccccgtcat cgagattgaa gattcttaca ggcccattta ttcaaattgg      720

agttgattct tcttgtcttt actttctttc tctctttttc ttcctttttt aatattatct      780

tttgtcaagc ctggttccct aagttgaact ctcttttctt gtgatcctcc tatatagata      840

cgccttgcca aatgcggccg cgagtccatc ggttcctgtc agatgggata ctcttgacgt      900

ggaaaattca aacagaaaaa aaaccccaat aatgaaaaat aacactacgt tatatccgtg      960

gtatcctcta tcgtatcgta tcgtagcgta tcgtagcgta ccgtatcaca gtatagtcta     1020

atattccgta tcttattgta tcctatccta ttcgatccta ttgtatttca gtgcaccatt     1080

ttaatttcta ttgctataat gtccttatta gttgccactg tgaggtgacc aatggacgag     1140

ggcgagccgt tcagaagccg cgaagggtgt tcttcccatg aatttcttaa ggagggcggc     1200

tcagctccga gagtgaggcg agacgtctcg gtcagcgtat cccccttcct cggcttttac     1260

aaatgatgcg ctcttaatag tgtgtcgtta tccttttggc attgacgggg gagggaaatt     1320

gattgagcgc atccatattt ttgcggactg ctgaggacaa tggtggtttt tccgggtggc     1380

gtgggctaca aatgatacga tggttttttt cttttcggag aaggcgtata aaaaggacac     1440

ggagaaccca tttattctaa aaacagttga gcttctttaa ttattttttg atataatatt     1500

ctattattat atattttctt cccaataaaa caaaataaaa caaaacacag caaaacacaa     1560

aaattctaga taaaatgtca actgtggaag atcactcctc cctacataaa ttgagaaagg     1620

aatctgagat tctttccaat gcaaacaaaa tcttagtggc taatagaggt gaaattccaa     1680

ttagaatttt caggtcagcc catgaattgt caatgcatac tgtggcgatc tattcccatg     1740

aagatcggtt gtccatgcat aggttgaagg ccgacgaggc ttatgcaatc ggtaagactg     1800

gtcaatattc gccagttcaa gcttatctac aaattgacga aattatcaaa atagcaaagg     1860

aacatgatgt ttccatgatc catccaggtt atggtttctt atctgaaaac tccgaattcg     1920

caaagaaggt tgaagaatcc ggtatgattt gggttgggcc tcctgctgaa gttattgatt     1980

ctgttggtga caaggtttct gcaagaaatt tggcaattaa atgtgacgtt cctgttgttc     2040

ctggtaccga tggtccaatt gaagacattg aacaggctaa acagtttgtg gaacaatatg     2100

gttatcctgt cattataaag gctgcatttg gtggtggtgg tagaggtatg agagttgtta     2160

gagaaggtga tgatatagtt gatgctttcc aaagagcgtc atctgaagca aagtctgcct     2220

ttggtaatgg tacttgtttt attgaaagat ttttggataa gccaaaacat attgaggttc     2280

aattattggc tgataattat ggtaacacaa tccatctctt tgaaagagat tgttctgttc     2340

aaagaagaca tcaaaaggtt gttgaaattg cacctgccaa aactttacct gttgaagtta     2400

gaaatgctat attaaaggat gctgtaacgt tagctaaaac cgctaactat agaaatgctg     2460

gtactgcaga atttttagtt gattcccaaa acagacatta ttttattgaa attaatccaa     2520

gaattcaagt tgaacataca attactgaag aaatcacggg tgttgatatt gttgccgctc     2580

aaattcaaat tgctgcaggt gcatcattgg aacaattggg tctattacaa aacaaaatta     2640

caactagagg ttttgcaatt caatgtagaa ttacaaccga ggatcctgct aagaattttg     2700

ccccagatac aggtaaaatt gaggtttata gatctgcagg tggtaacggt gtcagattag     2760

atggtggtaa tgggtttgcc ggtgctgtta tatctcctca ttatgactcg atgttggtta     2820

aatgttcaac atctggttct aactatgaaa ttgccagaag aaagatgatt agagctttag     2880

ttgaatttag aatcagaggt gtcaagacca atattccttt cttattggca ttgctaactc     2940

atccagtttt catttcgggt gattgttgga caacttttat tgatgatacc ccttcgttat     3000

tcgaaatggt ttcttcaaag aatagagccc aaaaattatt ggcatatatt ggtgacttgt     3060

gtgtcaatgg ttcttcaatt aaaggtcaaa ttggtttccc taaattgaac aaggaagcag     3120

aaatcccaga tttgttggat ccaaatgatg aggttattga tgtttctaaa ccttctacca     3180

atggtctaag accgtatcta ttaaagtatg gaccagatgc gttttccaaa aaagttcgtg     3240

aattcgatgg ttgtatgatt atggatacca cctggagaga tgcacatcaa tcattattgg     3300

ctacaagagt tagaactatt gatttactga gaattgctcc aacgactagt catgccttac     3360

aaaatgcatt tgcattagaa tgttggggtg gcgcaacatt tgatgttgcg atgaggttcc     3420

tctatgaaga tccttgggag agattaagac aacttagaaa ggcagttcca aatattcctt     3480

tccaaatgtt attgagaggt gctaatggtg ttgcttattc gtcattacct gataatgcaa     3540

ttgatcattt tgttaagcaa gcaaaggata atggtgttga tattttcaga gtctttgatg     3600

ctttgaacga tttggaacaa ttgaaggttg gtgttgatgc tgtcaagaaa gccggaggtg     3660

ttgttgaagc tacagtttgt tactcaggtg atatgttaat tccaggtaaa aagtataact     3720

tggattatta tttagagact gttggaaaga ttgtggaaat gggtacccat attttaggta     3780

ttaaggatat ggctggcacg ttaaagccaa aggctgctaa gttgttgatt ggctcgatca     3840

gatcaaaata ccctgacttg gttatccatg tccataccca tgactctgct ggtaccggta     3900

tttcaactta tgttgcatgc gcattggcag gtgccgacat tgtcgattgt gcaatcaatt     3960

cgatgtctgg tttaacctct caaccttcaa tgagtgcttt tattgctgct ttagatggtg     4020

atatcgaaac tggtgttcca gaacattttg caagacaatt agatgcatac tgggcagaaa     4080

tgagattgtt atactcatgt ttcgaagccg acttgaaggg accagaccca gaagtttata     4140

aacatgaaat tccaggtgga cagttgacta acctaatctt ccaagcccaa caagttggtt     4200

tgggtgaaca atgggaagaa actaagaaga agtatgaaga tgctaacatg ttgttgggtg     4260

atattgtcaa ggttacccca acctccaagg ttgttggtga tttagcccaa tttatggttt     4320

ctaataaatt agaaaaagaa gatgttgaaa aacttgctaa tgaattagat ttcccagatt     4380

cagttcttga tttctttgaa ggattaatgg gtacaccata tggtggattc ccagagcctt     4440

tgagaacaaa tgtcatttcc ggcaagagaa gaaaattaaa gggtagacca ggtttagaat     4500

tagaaccttt caacctcgag gaaatcagag aaaatttggt ttccagattt ggtccaggta     4560

ttactgaatg tgatgttgca tcttataaca tgtatccaaa ggtttacgag caatatcgta     4620

aggtggttga aaaatatggt gatttatctg ttttaccaac aaaagcattt ttggctcctc     4680

caactattgg tgaagaagtt catgtggaaa ttgagcaagg taagactttg attattaagt     4740

tattagccat ttctgacttg tctaaatctc atggtacaag agaagtatac tttgaattga     4800

atggtgaaat gagaaaggtt acaattgaag ataaaacagc tgcaattgag actgttacaa     4860

gagcaaaggc tgacggacac aatccaaatg aagttggtgc gccaatggct ggtgtcgttg     4920

ttgaagttag agtgaagcat ggaacagaag ttaagaaggg tgatccatta gccgttttga     4980

gtgcaatgaa aatggaaatg gttatttctg ctcctgttag tggtagggtc ggtgaagttt     5040

ttgtcaacga aggcgattcc gttgatatgg gtgatttgct tgtgaaaatt gccaaagatg     5100

aagcgccagc agcttaatta attctgtctt tgattttctt atgttattca aaacatctgc     5160

cccaaaatct aacgattata tatattccta cgtataactg tatagctaat tattgattta     5220

tttgtacata aaaaccacat aaatgtaaaa gcaagaaaaa aaataactaa ggagaaggat     5280

caatatctca tttataatgc tcgccaaagc agcgtacgtg aattttaatc aagacatcaa     5340

caaatcttgc aacttggtta tatcgcttct tcacccactc acccgctttt ctacattgtt     5400

gaacacaaat atatacaggg gtatgtctca aggtcaagtg cagtttcaac agagactacc     5460

tcaaggtacc tcttcagaaa tgcagaactt cactcttgat cagattttct ccgaattaaa     5520

ggtttaaaca tagcctcatg aaatcagcca tttgcttttg ttcaacgatc ttttgaaatt     5580

gttgttgttc ttggtagtta agttgatcca tcttggctta tgttgtgtgt atgttgtagt     5640

tattcttagt atattcctgt cctgagttta gtgaaacata atatcgcctt gaaatgaaaa     5700

tgctgaaatt cgtcgacata caatttttca aacttttttt ttttcttggt gcacggacat     5760

gtttttaaag gaagtactct ataccagtta ttcttcacaa atttaattgc tggagaatag     5820

atcttcaacg ctttaataaa gtagtttgtt tgtcaaggat ggcgtcatac aaagaaagat     5880

cagaatcaca cacttcccct gttgctagga gacttttctc catcatggag gaaaagaagt     5940

ctaacctttg tgcatcattg gatattactg aaactgaaaa gcttctctct attttggaca     6000

ctattggtcc ttacatctgt ctagttaaaa cacacatcga tattgtttct gattttacgt     6060

atgaaggaac tgtgttgcct ttgaaggagc ttgccaagaa acataatttt atgatttttg     6120

aagatagaaa atttgctgat attggtaaca ctgttaaaaa tcaatataaa tctggtgtct     6180

tccgtattgc cgaatgggct gacatcacta atgcacatgg tgtaacgggt gcaggtattg     6240

tttctggctt gaaggaggca gcccaagaaa caaccagtga acctagaggt ttgctaatgc     6300

ttgctgagtt atcatcaaag ggttctttag catatggtga atatacagaa aaaacagtag     6360

aaattgctaa atctgataaa gagtttgttg ag                                   6392


<210>  154
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  154
gcaggtgctt caggtcaatt cg                                                22


<210>  155
<211>  4886
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MAE gene integration fragment

<400>  155
ctttgaagga gcttgccaag aaacataatt ttatgatttt tgaagataga aaatttgctg       60

atattggtaa cactgttaaa aatcaatata aatctggtgt cttccgtatt gccgaatggg      120

ctgacatcac taatgcacat ggtgtaacgg gtgcaggtat tgtttctggc ttgaaggagg      180

cagcccaaga aacaaccagt gaacctagag gtttgctaat gcttgctgag ttatcatcaa      240

agggttcttt agcatatggt gaatatacag aaaaaacagt agaaattgct aaatctgata      300

aagagtttgt cattggtttt attgcgcaac acgatatggg cggtagagaa gaaggttttg      360

actggatcat tatgactcca ggggttggtt tagatgacaa aggtgatgca cttggtcaac      420

aatatagaac tgttgatgaa gttgtaaaga ctggaacgga tatcataatt gttggtagag      480

gtttgtacgg tcaaggaaga gatcctatag agcaagctaa aagataccaa caagctggtt      540

ggaatgctta tttaaacaga tttaaatgat tcttacacaa agatttgata catgtacact      600

agtttaaata agcatgaaaa gaattacaca agcaaaaaaa aaaaaataaa tgaggtactt      660

tacgttcacc tacaaccaaa aaaactagat agagtaaaat cttaagattt agaaaaagtt      720

gtttaacaaa ggctttagta tgtgaatttt taatgtagca aagcgataac taataaacat      780

aaacaaaagt atggttttct ttatcagtca aatcattatc gattgattgt tccgcgtatc      840

tgcagatagc ctcatgaaat cagccatttg cttttgttca acgatctttt gaaattgttg      900

ttgttcttgg tagttaagtt gatccatctt ggcttatgtt gtgtgtatgt tgtagttatt      960

cttagtatat tcctgtcctg agtttagtga aacataatat cgccttgaaa tgaaaatgct     1020

gaaattcgtc gacatacaat ttttcaaact tttttttttt cttggtgcac ggacatgttt     1080

ttaaaggaag tactctatac cagttattct tcacaaattt aattgctgga gaatagatct     1140

tcaacgcgtt taaacagcaa tttgaggaag gaataggaga aggagaagca atttctagga     1200

aagagcaagg tgtgcaacag catgctctga atgatatttt cagcaatagt tcagttgaag     1260

aacctgttgg cgtatctaca tcacttccta caaacaacac cacgaattgc gtccgtggtg     1320

acgcaactac gaatggcatt gtcaatgcca atgccagtgc acatacacgt gcaagtccca     1380

ccggttccct gcccggctat ggtagagaca agaaggacga taccggcatc gacatcaaca     1440

gtttcaacag caatgcgttt ggcgtcgacg cgtcgatggg gctgccgtat ttggatttgg     1500

acgggctaga tttcgatatg gatatggata tggatatgga tatggagatg aatttgaatt     1560

tagatttggg tcttgatttg gggttggaat taaaagggga taacaatgag ggttttcctg     1620

ttgatttaaa caatggacgt gggaggtgat tgatttaacc tgatccaaaa ggggtatgtc     1680

tattttttag agtgtgtctt tgtgtcaaat tatggtagaa tgtgtaaagt agtataaact     1740

ttcctctcaa atgacgaggt ttaaaacacc ccccgggtga gccgagccga gaatggggca     1800

attgttcaat gtgaaataga agtatcgagt gagaaacttg ggtgttggcc agccaagggg     1860

gaaggaaaat ggcgcgaatg ctcaggtgag attgttttgg aattgggtga agcgaggaaa     1920

tgagcgaccc ggaggttgtg actttagtgg cggaggagga acgggaggaa aaggccaaga     1980

gggaaagtgt atataagggg gagcaatttg ccaaccagga tagaattgga tgagttataa     2040

ttctactgta tttattgtat aatttatttc tccttttata tcaaacacat tacaaaacac     2100

acaaaacata caaacataca cagctagcat gggtgaattg aaagagattt tgaaacaaag     2160

atatcatgaa ttacttgatt ggaatgttaa ggcaccacat gtccctttat cccagagatt     2220

gaagcacttt acttggtcat ggtttgcttg tactatggca accggtggtg ttggtttgat     2280

cattggttcc ttcccattca gattctacgg tttgaacacc attggcaaga ttgtttacat     2340

cttacaaatc tttttgtttt ctctttttgg ctcttgtatg ttgtttcgtt tcatcaagta     2400

tccatctacc attaaggact cttggaatca tcacttggaa aagttgttta tcgcaacttg     2460

tttgttatct atttccacat tcatcgacat gttagctatc tatgcttatc cagataccgg     2520

tgaatggatg gtctgggtca ttagaatctt atactacatc tatgtcgctg tctctttcat     2580

ctactgtgtt atggcctttt tcaccatttt caacaatcat gtttacacta ttgaaactgc     2640

ttctccagct tggattttgc caatcttccc tccaatgatc tgtggtgtca ttgctggtgc     2700

tgttaactcc acccaacctg ctcaccaatt gaaaaacatg gtcattttcg gtatcttgtt     2760

tcaaggttta ggtttttggg tttacctttt acttttcgcc gttaatgttt tgagattctt     2820

cacagtcggt ttagcaaagc cacaagatag accaggtatg tttatgttcg ttggtccacc     2880

agctttctct ggtttagcat tgattaacat tgcaagaggt gcaatgggct caagacctta     2940

cattttcgtt ggtgcaaact cttccgaata cttaggtttt gtctcaacct tcatggccat     3000

tttcatctgg ggtttagccg catggtgtta ttgcttagct atggtttcct tccttgccgg     3060

ctttttcact agagcaccat tgaaattcgc ttgtggttgg ttcgctttca tctttccaaa     3120

tgttggtttt gttaactgta ctatcgaaat cggcaagatg attgattcta aggcttttca     3180

aatgtttggt cacatcattg gtgttatctt gtgtattcaa tggattttgt taatgtactt     3240

aatggttaga gcattccttg ttaatgactt gtgctatcct ggtaaagacg aagatgcaca     3300

cccaccacca aagccaaaca ctggtgtctt aaacccaact ttcccaccag agaaggctcc     3360

agcatcatta gagaaggttg atactcatgt tacatcaaca ggtggtgaat ccgatcctcc     3420

atcttccgaa catgaatccg tttaaggcgc gccatctaat agtttaatca cagcttatag     3480

tctactatag ttttcttttt taaacattgt tgtattttgt cccccccctc taattgatga     3540

tgattatcct ataagaatcc aataaaacga tggaaactaa taccctctcc tttgtcatgt     3600

ggtctttagt atttcttgaa cattggctct gatttctcga ctttatagtc ctattaaaat     3660

cgctgttagt tctcgatcgt tgtatctcgt ttcttgtctc tttggtggat gattttgcgt     3720

gcgaacatgt ttttttccct ttctctcacc atcatcgtgt agttcttgtc accatccccc     3780

ccaccccttc cttctctcat tgattctata agagcttatc cacagaggtg cagtaacgag     3840

gtagtttaac cttcgagtgg atcaaaatgt cacacaggcc tgcggccgct accataatgt     3900

atgcgttgag cctcttgcac cttctttatt aggaaatcag ttgaaaaatt tccggattgt     3960

ctttattatt ggcccatttt tttttggtca cacctttatt tttgtacact tctcgggcaa     4020

agcaaaaact atagtaccgg ataggccttt ataaaactcc agtgtgtatg attttagttg     4080

gtgtgccatc tacacgttct cttagtttct ttatcatgtc acagaaagca agcatgcaaa     4140

cccttacaaa aaataacaac atacaaatgc ctaaacaact ggactataat gatggtgagt     4200

cagttacgaa aagagcaagt gggttaatac gatttcgtaa gggacagtct gaggaagact     4260

acaattttca aaaggagcag ttctggtcca cgggtccttt agtacagaat cacacatttg     4320

tgactgaatt tgttgaaaag tttattgaaa acacaattag tgaagattat tcaatcacag     4380

atagatcgaa aatagaacgt gaaacaatca tacacggatt ggagaagctg tattttcaaa     4440

gggaatatga gcgatgtcta aaagatgttc aactattgaa ggacaatatc gataagttca     4500

atcctaattt ggatcttaat gaaaagaatt tataatgagc tgaattatat ttcttggatg     4560

tgcatcaaaa agatccatga gagtaacgaa aagaaactgg gggaaatcta ataatttaca     4620

atttcaatat acacttctat atcctttaat gtaatggctt tataaataaa cacgaacttc     4680

tacagcaccg acgtttcttt ttcttaccag ctcctcttct tcttcttctt cttcttcttc     4740

ttcttcttct tcttcttctt cttcttcttc ttcttcttct ttcttaccat cattgccatt     4800

ttcctttttt cttatttgct cttgatcctc tgttttttca atttggacaa actcatctaa     4860

tacaccaaca cttttagggc ccccgc                                          4886


<210>  156
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  156
cagcagcacc acaaactgta ac                                                22


<210>  157
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  157
tcttctctac cgcccatatc                                                   20


<210>  158
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  158
tgaatgggag gcatgaatcg cag                                               23


<210>  159
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  159
cacagaggtg cagtaacgag                                                   20


<210>  160
<211>  4115
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I. orientalis FUM gene integration fragment

<400>  160
aattctttga aggagcttgc caagaaacat aattttatga tttttgaaga tagaaaattt       60

gctgatattg gtaacactgt taaaaatcaa tataaatctg gtgtcttccg tattgccgaa      120

tgggctgaca tcactaatgc acatggtgta acgggtgcag gtattgtttc tggcttgaag      180

gaggcagccc aagaaacaac cagtgaacct agaggtttgc taatgcttgc tgagttatca      240

tcaaagggtt ctttagcata tggtgaatat acagaaaaaa cagtagaaat tgctaaatct      300

gataaagagt ttgtcattgg ttttattgcg caacacgata tgggcggtag agaagaaggt      360

tttgactgga tcattatgac tccaggggtt ggtttagatg acaaaggtga tgcacttggt      420

caacaatata gaactgttga tgaagttgta aagactggaa cggatatcat aattgttggt      480

agaggtttgt acggtcaagg aagagatcct atagagcaag ctaaaagata ccaacaagct      540

ggttggaatg cttatttaaa cagatttaaa tgattcttac acaaagattt gatacatgta      600

cactagttta aataagcatg aaaagaatta cacaagcaaa aaaaaaaaaa taaatgaggt      660

actttacgtt cacctacaac caaaaaaact agatagagta aaatcttaag atttagaaaa      720

agttgtttaa caaaggcttt agtatgtgaa tttttaatgt agcaaagcga taactaataa      780

acataaacaa aagtatggtt ttctttatca gtcaaatcat tatcgattga ttgttccgcg      840

tatctgcaga tagcctcatg aaatcagcca tttgcttttg ttcaacgatc ttttgaaatt      900

gttgttgttc ttggtagtta agttgatcca tcttggctta tgttgtgtgt atgttgtagt      960

tattcttagt atattcctgt cctgagttta gtgaaacata atatcgcctt gaaatgaaaa     1020

tgctgaaatt cgtcgacata caatttttca aacttttttt ttttcttggt gcacggacat     1080

gtttttaaag gaagtactct ataccagtta ttcttcacaa atttaattgc tggagaatag     1140

atcttcaacg cgtttcctcg acatttgctg caacggcaac atcaatgtcc acgtttacac     1200

acctacattt atatctatat ttatatttat atttatttat ttatgctact tagcttctat     1260

agttagttaa tgcactcacg atattcaaaa ttgacaccct tcaactactc cctactattg     1320

tctactactg tctactactc ctctttacta tagctgctcc caataggctc caccaatagg     1380

ctctgtcaat acattttgcg ccgccacctt tcaggttgtg tcactcctga aggaccatat     1440

tgggtaatcg tgcaatttct ggaagagagt ccgcgagaag tgaggccccc actgtaaatc     1500

ctcgaggggg catggagtat ggggcatgga ggatggagga tggggggggg gggggggaaa     1560

ataggtagcg aaaggacccg ctatcacccc acccggagaa ctcgttgccg ggaagtcata     1620

tttcgacact ccggggagtc tataaaaggc gggttttgtc ttttgccagt tgatgttgct     1680

gagaggactt gtttgccgtt tcttccgatt taacagtata gaatcaacca ctgttaatta     1740

tacacgttat actaacacaa caaaaacaaa aacaacgaca acaacaacaa catctagata     1800

aaatgttagc tgctagatca ttaaaggcaa gaatgtcaac aagagctttc tcaactacct     1860

caattgcaaa aagaatcgaa aaagatgcat ttggtgacat tgaagtccca aatgagaaat     1920

attggggtgc tcaaactcaa agatctttac aaaatttcaa aattggtggt aagagagaag     1980

ttatgccaga accaatcatc aaatcttttg gtattttaaa gaaggctact gctaagatca     2040

atgctgagtc tggtgcttta gacccaaagt tatctgaagc catccaacaa gctgcaaccg     2100

aagtttatga aggtaaacta atggaccatt tcccattagt tgtctttcaa accggttctg     2160

gtactcaatc taacatgaat gccaatgaag tcatctctaa tagagcaatt gaaatcttgg     2220

gtggtgaatt aggctctaaa actccagtcc atcctaatga tcatgttaat atgtcccaat     2280

cttctaatga tactttccct actgtcatgc atattgcagc agttacagaa gtttcatccc     2340

atttattacc agaattaact gcactaagag atgcattgca aaagaaatcc gatgaattta     2400

agaatattat caaaatcggt agaacccatt tacaagatgc aactccttta actttaggtc     2460

aagaattttc tggttatgtt caacaatgta ctaatggtat caaaagaatc gaaattgctc     2520

ttgaacattt gagatactta gctcaaggtg gtactgccgt tggtactggt cttaacacca     2580

agaaaggttt tgctgaaaag gttgcaaatg aagtcactaa attgactggt ttacaattct     2640

ataccgctcc aaataaattc gaagcccttg cagctcacga tgctgttgtt gaaatgtctg     2700

gtgctttgaa taccgttgca gtctcattat tcaaaatcgc tcaagatatc agatatttgg     2760

gttccggccc aagatgtggt tatggtgaat tggctttacc agaaaatgaa ccaggttctt     2820

ccatcatgcc gggtaaagtt aacccaactc aaaacgaagc tttgactatg ctttgtaccc     2880

aagtctttgg taaccactct tgtattacct ttgcaggtgc ttcaggtcaa ttcgaattga     2940

atgtctttaa gccagttatg atctccaact tgttatcttc tattaggtta ttaggtgatg     3000

gttgtaattc ttttagaatc cactgtgttg aaggtatcat tgcaaatacc gacaagattg     3060

ataaattact acatgaatct ctcatgttag ttactgcttt gaacccacac attggttacg     3120

ataaggcttc caagattgca aagaatgcac acaagaaggg cttgacattg aaacaatctg     3180

cattggaatt aggttacttg accgaagaac aattcaatga atgggttaga ccagaaaaca     3240

tgattggtcc aaaggattaa gttaattaac atctgaatgt aaaatgaaca ttaaaatgaa     3300

ttactaaact ttacgtctac tttacaatct ataaactttg tttaatcata taacgaaata     3360

cactaataca caatcctgta cgtatgtaat acttttatcc atcaaggatt gagaaaaaaa     3420

agtaatgatt ccctgggcca ttaaaactta gacccccaag cttggatagg tcactctcta     3480

ttttcgtttc tcccttccct gatagaaggg tgatatgtaa ttaagaataa tatataattt     3540

tataataaaa gcggccgcct cccttctcta aatggactgc ttggataact tggaccccct     3600

tcccatttta tagtcattct cttccccctc attttcccac tattcccaac aatgaccatc     3660

tctccacctt gtttccccat tcttcctgct ctacctggtg ggggtgtttc accccattaa     3720

cggtcggatt ccgctgtgga gatggctctg gcctttttcc cattccttcc ccccctcaat     3780

cttctccatg cggggaaaaa aaaattttat ccataaacaa ccaaaccggc ggctcaacgg     3840

ggggtttata ctgacagaaa tggggtcaat acacccactg actgtacccg ctctaatctt     3900

aagctttccc ccccccctcc tgtattaacg gcgcggagtg cccgcagcgc ccaatggaga     3960

aggcgcgcag tgggggatgc ccagggaggg gacaggtaca cgcacaggcc atgccaacac     4020

cgcatagacg tgcgacctcc tctcccccac tgcagagctg cccttttcgg acacactccg     4080

tgcaagagga ctcggccggc tcggcttttc tgccg                                4115


<210>  161
<211>  6527
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PYC gene inserter fragment

<400>  161
ctaaaagtgt tggtgtatta gatgagtttg tccaaattga aaaaacagag gatcaagagc       60

aaataagaaa aaaggaaaat ggcaatgatg gtaagaaaga agaagaagaa gaagaagaag      120

aagaagaaga agaagaagaa gaagaagaag aagaagaaga agaggagctg gtaagaaaaa      180

gaaacgtcgg tgctgtagaa gttcgtgttt atttataaag ccattacatt aaaggatata      240

gaagtgtata ttgaaattgt aaattattag atttccccca gtttcttttc gttactctca      300

tggatctttt tgatgcacat ccaagaaata taattcagct cattataaat tcttttcatt      360

aagatccaaa ttaggattga acttatcgat attgtccttc aatagttgaa catcttttag      420

acatcgctca tattcccttt gaaaatacag cttctccaat ccgtgtatga ttgtttcacg      480

ttctattttc gatctatctg tgattgaata atcttcacta attgtgtttt caataaactt      540

ttcaacaaat tcagtcacaa atgtgtgatt ctgtactaaa ggacccgtgg accagaactg      600

ctccttttga aaattgtagt cttcctcaga ctgtccctta cgaaatcgta ttaacccact      660

tgctcttttc gtaactgact caccatcatt atagtccagt tgtttaggca tttgtatgtt      720

gttatttttt gtaagggttt gcatgcttgc tttctgtgac atgataaaga aactaagaga      780

acgtgtagat ggcacaccaa ctaaaatcat acacactgga gttttataaa ggcctatccg      840

gtactatagt ttttgctttg cccgagaagt gtacaaaaat aaaggtgtga ccaaaaaaaa      900

atgggccaat aataaagaca atccggaaat ttttcaactg atttcctaat aaagaaggtg      960

caagaggctc aacgcataca ttatggtagc ggccgcgagt ccatcggttc ctgtcagatg     1020

ggatactctt gacgtggaaa attcaaacag aaaaaaaacc ccaataatga aaaataacac     1080

tacgttatat ccgtggtatc ctctatcgta tcgtatcgta gcgtatcgta gcgtaccgta     1140

tcacagtata gtctaatatt ccgtatctta ttgtatccta tcctattcga tcctattgta     1200

tttcagtgca ccattttaat ttctattgct ataatgtcct tattagttgc cactgtgagg     1260

tgaccaatgg acgagggcga gccgttcaga agccgcgaag ggtgttcttc ccatgaattt     1320

cttaaggagg gcggctcagc tccgagagtg aggcgagacg tctcggtcag cgtatccccc     1380

ttcctcggct tttacaaatg atgcgctctt aatagtgtgt cgttatcctt ttggcattga     1440

cgggggaggg aaattgattg agcgcatcca tatttttgcg gactgctgag gacaatggtg     1500

gtttttccgg gtggcgtggg ctacaaatga tacgatggtt tttttctttt cggagaaggc     1560

gtataaaaag gacacggaga acccatttat tctaaaaaca gttgagcttc tttaattatt     1620

ttttgatata atattctatt attatatatt ttcttcccaa taaaacaaaa taaaacaaaa     1680

cacagcaaaa cacaaaaatt ctagataaaa tgtcaactgt ggaagatcac tcctccctac     1740

ataaattgag aaaggaatct gagattcttt ccaatgcaaa caaaatctta gtggctaata     1800

gaggtgaaat tccaattaga attttcaggt cagcccatga attgtcaatg catactgtgg     1860

cgatctattc ccatgaagat cggttgtcca tgcataggtt gaaggccgac gaggcttatg     1920

caatcggtaa gactggtcaa tattcgccag ttcaagctta tctacaaatt gacgaaatta     1980

tcaaaatagc aaaggaacat gatgtttcca tgatccatcc aggttatggt ttcttatctg     2040

aaaactccga attcgcaaag aaggttgaag aatccggtat gatttgggtt gggcctcctg     2100

ctgaagttat tgattctgtt ggtgacaagg tttctgcaag aaatttggca attaaatgtg     2160

acgttcctgt tgttcctggt accgatggtc caattgaaga cattgaacag gctaaacagt     2220

ttgtggaaca atatggttat cctgtcatta taaaggctgc atttggtggt ggtggtagag     2280

gtatgagagt tgttagagaa ggtgatgata tagttgatgc tttccaaaga gcgtcatctg     2340

aagcaaagtc tgcctttggt aatggtactt gttttattga aagatttttg gataagccaa     2400

aacatattga ggttcaatta ttggctgata attatggtaa cacaatccat ctctttgaaa     2460

gagattgttc tgttcaaaga agacatcaaa aggttgttga aattgcacct gccaaaactt     2520

tacctgttga agttagaaat gctatattaa aggatgctgt aacgttagct aaaaccgcta     2580

actatagaaa tgctggtact gcagaatttt tagttgattc ccaaaacaga cattatttta     2640

ttgaaattaa tccaagaatt caagttgaac atacaattac tgaagaaatc acgggtgttg     2700

atattgttgc cgctcaaatt caaattgctg caggtgcatc attggaacaa ttgggtctat     2760

tacaaaacaa aattacaact agaggttttg caattcaatg tagaattaca accgaggatc     2820

ctgctaagaa ttttgcccca gatacaggta aaattgaggt ttatagatct gcaggtggta     2880

acggtgtcag attagatggt ggtaatgggt ttgccggtgc tgttatatct cctcattatg     2940

actcgatgtt ggttaaatgt tcaacatctg gttctaacta tgaaattgcc agaagaaaga     3000

tgattagagc tttagttgaa tttagaatca gaggtgtcaa gaccaatatt cctttcttat     3060

tggcattgct aactcatcca gttttcattt cgggtgattg ttggacaact tttattgatg     3120

ataccccttc gttattcgaa atggtttctt caaagaatag agcccaaaaa ttattggcat     3180

atattggtga cttgtgtgtc aatggttctt caattaaagg tcaaattggt ttccctaaat     3240

tgaacaagga agcagaaatc ccagatttgt tggatccaaa tgatgaggtt attgatgttt     3300

ctaaaccttc taccaatggt ctaagaccgt atctattaaa gtatggacca gatgcgtttt     3360

ccaaaaaagt tcgtgaattc gatggttgta tgattatgga taccacctgg agagatgcac     3420

atcaatcatt attggctaca agagttagaa ctattgattt actgagaatt gctccaacga     3480

ctagtcatgc cttacaaaat gcatttgcat tagaatgttg gggtggcgca acatttgatg     3540

ttgcgatgag gttcctctat gaagatcctt gggagagatt aagacaactt agaaaggcag     3600

ttccaaatat tcctttccaa atgttattga gaggtgctaa tggtgttgct tattcgtcat     3660

tacctgataa tgcaattgat cattttgtta agcaagcaaa ggataatggt gttgatattt     3720

tcagagtctt tgatgctttg aacgatttgg aacaattgaa ggttggtgtt gatgctgtca     3780

agaaagccgg aggtgttgtt gaagctacag tttgttactc aggtgatatg ttaattccag     3840

gtaaaaagta taacttggat tattatttag agactgttgg aaagattgtg gaaatgggta     3900

cccatatttt aggtattaag gatatggctg gcacgttaaa gccaaaggct gctaagttgt     3960

tgattggctc gatcagatca aaataccctg acttggttat ccatgtccat acccatgact     4020

ctgctggtac cggtatttca acttatgttg catgcgcatt ggcaggtgcc gacattgtcg     4080

attgtgcaat caattcgatg tctggtttaa cctctcaacc ttcaatgagt gcttttattg     4140

ctgctttaga tggtgatatc gaaactggtg ttccagaaca ttttgcaaga caattagatg     4200

catactgggc agaaatgaga ttgttatact catgtttcga agccgacttg aagggaccag     4260

acccagaagt ttataaacat gaaattccag gtggacagtt gactaaccta atcttccaag     4320

cccaacaagt tggtttgggt gaacaatggg aagaaactaa gaagaagtat gaagatgcta     4380

acatgttgtt gggtgatatt gtcaaggtta ccccaacctc caaggttgtt ggtgatttag     4440

cccaatttat ggtttctaat aaattagaaa aagaagatgt tgaaaaactt gctaatgaat     4500

tagatttccc agattcagtt cttgatttct ttgaaggatt aatgggtaca ccatatggtg     4560

gattcccaga gcctttgaga acaaatgtca tttccggcaa gagaagaaaa ttaaagggta     4620

gaccaggttt agaattagaa cctttcaacc tcgaggaaat cagagaaaat ttggtttcca     4680

gatttggtcc aggtattact gaatgtgatg ttgcatctta taacatgtat ccaaaggttt     4740

acgagcaata tcgtaaggtg gttgaaaaat atggtgattt atctgtttta ccaacaaaag     4800

catttttggc tcctccaact attggtgaag aagttcatgt ggaaattgag caaggtaaga     4860

ctttgattat taagttatta gccatttctg acttgtctaa atctcatggt acaagagaag     4920

tatactttga attgaatggt gaaatgagaa aggttacaat tgaagataaa acagctgcaa     4980

ttgagactgt tacaagagca aaggctgacg gacacaatcc aaatgaagtt ggtgcgccaa     5040

tggctggtgt cgttgttgaa gttagagtga agcatggaac agaagttaag aagggtgatc     5100

cattagccgt tttgagtgca atgaaaatgg aaatggttat ttctgctcct gttagtggta     5160

gggtcggtga agtttttgtc aacgaaggcg attccgttga tatgggtgat ttgcttgtga     5220

aaattgccaa agatgaagcg ccagcagctt aattaattct gtctttgatt ttcttatgtt     5280

attcaaaaca tctgccccaa aatctaacga ttatatatat tcctacgtat aactgtatag     5340

ctaattattg atttatttgt acataaaaac cacataaatg taaaagcaag aaaaaaaata     5400

actaaggaga aggatcaata tctcatttat aatgctcgcc aaagcagcgt acgtgaattt     5460

taatcaagac atcaacaaat cttgcaactt ggttatatcg cttcttcacc cactcacccg     5520

cttttctaca ttgttgaaca caaatatata caggggtatg tctcaaggtc aagtgcagtt     5580

tcaacagaga ctacctcaag gtacctcttc agaaatgcag aacttcactc ttgatcagat     5640

tttctccgaa ttaaaggttt aaacatagcc tcatgaaatc agccatttgc ttttgttcaa     5700

cgatcttttg aaattgttgt tgttcttggt agttaagttg atccatcttg gcttatgttg     5760

tgtgtatgtt gtagttattc ttagtatatt cctgtcctga gtttagtgaa acataatatc     5820

gccttgaaat gaaaatgctg aaattcgtcg acatacaatt tttcaaactt tttttttttc     5880

ttggtgcacg gacatgtttt taaaggaagt actctatacc agttattctt cacaaattta     5940

attgctggag aatagatctt caacgcttta ataaagtagt ttgtttgtca aggatggcgt     6000

catacaaaga aagatcagaa tcacacactt cccctgttgc taggagactt ttctccatca     6060

tggaggaaaa gaagtctaac ctttgtgcat cattggatat tactgaaact gaaaagcttc     6120

tctctatttt ggacactatt ggtccttaca tctgtctagt taaaacacac atcgatattg     6180

tttctgattt tacgtatgaa ggaactgtgt tgcctttgaa ggagcttgcc aagaaacata     6240

attttatgat ttttgaagat agaaaatttg ctgatattgg taacactgtt aaaaatcaat     6300

ataaatctgg tgtcttccgt attgccgaat gggctgacat cactaatgca catggtgtaa     6360

cgggtgcagg tattgtttct ggcttgaagg aggcagccca agaaacaacc agtgaaccta     6420

gaggtttgct aatgcttgct gagttatcat caaagggttc tttagcatat ggtgaatata     6480

cagaaaaaac agtagaaatt gctaaatctg ataaagagtt tgttgag                   6527


<210>  162
<211>  3863
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MDH gene integration fragment

<400>  162
ccataactga catttatggt aaggttgctc gttgtaatgt agtgttgtgt ggtatggtat       60

tatagtataa aatagtatat caataatata tcggagtata tatggtataa tagtatgaag      120

tatagcacat tgtaccatgt cgtggagcat attatggtat tacgtggttt ggcatagtat      180

actatagtat tatagagttg tattgtacta taaagcagtg ttgtttctgg atactacact      240

aacgtatagt gtactacagt tttgtatagt atagtctagc gtatcagagt tgtactgcag      300

catcgtgtag tatgctacat tgaactcctc atgtcgcatc ttatattatc gtattgatct      360

catcttatct tatcatatca tattatttca tttcatttcg ttatatactc tactcctcat      420

cactatacat cccccatgca tatcaatata cacataaaaa cgtcacacca gtacacccaa      480

gtcagcccat catttcgaag tgtcaacaac atatctgcct aaaatcttac cttgctccat      540

caattcatag atctttggca actcactcaa accaacaatc ttaattggtg acttcactaa      600

acccctggtg cggccgcgga tccctcgaga ttggtagttc tttccccctc tcaagctggc      660

gtgaaatgca accttacggc gtctacgtta ctacaaggtc cagaaagtgt aggtattgct      720

actattttta ttttttattg gttctggaga aatgcagaca gtcaatgaac acaactgtct      780

caatatgcat ctatgcacat gcacacacac acacatcaca ggtaccccta caaagagagg      840

tctcttgata atgtttcatt accacgtggc atcccccccc ccccccccaa taaacaagtg      900

gccgagttcc cctgttgcag aggaggacaa aaaaaccgct ggtgttggta ccattatgca      960

gcaactagca caacaaacaa ccgacccaga catacaaatc aacaacactt cgccaaagac     1020

accctttcca gggaggatcc actcccaacg tctctccata atgtctctgt tggcccatgt     1080

ctctgtcgtt gacaccgtaa ccacaccaac caacccgtcc attgtactgg gatggtcgtc     1140

catagacacc tctccaacgg ggaacacctc attcgtaaac cgccaaggtt accgttcctc     1200

ctgactcgcc ccgttgttga tgctgcgcac ctgtggttgc ccaacatggt tgtatatcgt     1260

gtaaccacac caacacatgt gcagcacatg tgtttaaaag agtgtcatgg aggtggatca     1320

tgatggaagt ggactttacc acttgggaac tgtctccact cccgggaaga aaagacccgg     1380

cgtatcacgc ggttgcctca atggggcaat ttggaaggag aaatataggg aaaatcacgt     1440

cgctctcgga cggggaagag ttccagacta tgaggggggg gggtggtata taaagacagg     1500

agatgtccac ccccagagag aggaagaagt tggaacttta gaagagagag ataactttcc     1560

ccagtgtcca tcaatacaca accaaacaca aactctatat ttacacatat aaccccctct     1620

ctagataaaa tggttaaagt tacagtttgt ggtgctgctg gtggtattgg tcaacccctt     1680

tctttactct tgaagcaatc ctctcacatt actcacttat ctctttatga tatcgttaat     1740

actcctggtg ttgctgctga tcttagtcat atcgatacca aatccaaggt cactggtcat     1800

gtaggtgctg ctcaacttga agaagctatc aaggattctg atgttgtcgt tattcccgct     1860

ggtgtcccaa gaaagccagg tatgacgcgt gatgatcttt tcaagattaa tgctggtatt     1920

gtacgtgatt tggctacagc tgctgcaaag tacgctccaa aggccttcat gtgtatcatt     1980

tctaacccag tcaactcgac tgtcccaatc gttactgaag tattcaaaca gcacaatgtt     2040

tatgacccca aaagaatctt tggtgtaaca acacttgata ttgttcgtgc atccaccttt     2100

gtatccgaat tgattggagg tgaacctaat tcacttcgtg ttcccgtcat tggtggtcac     2160

agcggcgtaa ccatcttacc tttactctca caggtccccg gcattgaaaa gttaaaccaa     2220

gaacaaattg agaaggtaac tcatcgtatt caatttggtg gcgatgaagt tgtcaaggcc     2280

aaggatggtg ctggttctgc cactctttcc atggcttatg ctggtgctcg ttttgctaca     2340

aacatcattg aggctgcttt tgctggaaag aagggcattg ttgaatgtac ctatgttcaa     2400

ttggatgctg ataaatctgg tgcccaatct gtcaaggatt tggttggtag tgaacttgaa     2460

tatttctctg ttcccgttga attgggtcct agtggtgttg aaaagatttt acccattgga     2520

aacgttaatg aatatgaaaa gaagttgttg aacgaggctt ctcctgaatt aaaaaccaac     2580

attgataaag gttgtacttt tgttactgaa ggctcaaagt tgtaattaat taatttattt     2640

tactagttta tttttgctcc tgagaatagg attacaaaca cttaaagtct ttaattacaa     2700

ctatatataa tattctgttg gttttcttga attggttcgc tgcgattcat gcctcccatt     2760

caccaaaggt ggagtgggaa ataacggttt tactgcggta attagcagag gcaagaacag     2820

gatacacttt ttgatgataa atctgtatta tagtcgagcc tatttaggaa atcaaatttt     2880

cttgtgttta cttttcaaat aaataatgtt cgaaaatttt tactttactc cttcatttaa     2940

ctataccaga cgttatatca tcaacacctt ctgaccatat acagctcaag atgtttaaga     3000

gtctgttaaa ttttttcaat ccatttcatg gagtaccagg aggtgctaca aaaggaattc     3060

atagcctcat gaaatcagcc atttgctttt gttcaacgat cttttgaaat tgttgttgtt     3120

cttggtagtt aagttgatcc atcttggctt atgttgtgtg tatgttgtag ttattcttag     3180

tatattcctg tcctgagttt agtgaaacat aatatcgcct tgaaatgaaa atgctgaaat     3240

tcgtcgacat acaatttttc aaactttttt tttttcttgg tgcacggaca tgtttttaaa     3300

ggaagtactc tataccagtt attcttcaca aatttaattg ctggagaata gatcttcaac     3360

gctttaataa agtagtttgt ttgtcaagga tggcgtcata caaagaaaga tcagaatcac     3420

acacttcccc tgttgctagg agacttttct ccatcatgga ggaaaagaag tctaaccttt     3480

gtgcatcatt ggatattact gaaactgaaa agcttctctc tattttggac actattggtc     3540

cttacatctg tctagttaaa acacacatcg atattgtttc tgattttacg tatgaaggaa     3600

ctgtgttgcc tttgaaggag cttgccaaga aacataattt tatgattttt gaagatagaa     3660

aatttgctga tattggtaac actgttaaaa atcaatataa atctggtgtc ttccgtattg     3720

ccgaatgggc tgacatcact aatgcacatg gtgtaacggg tgcaggtatt gtttctggct     3780

tgaaggaggc agcccaagaa acaaccagtg aacctagagg tttgctaatg cttgctgagt     3840

tatcatcaaa gggttcttta gca                                             3863


<210>  163
<211>  4760
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MAE gene integration fragment

<400>  163
aattctttga aggagcttgc caagaaacat aattttatga tttttgaaga tagaaaattt       60

gctgatattg gtaacactgt taaaaatcaa tataaatctg gtgtcttccg tattgccgaa      120

tgggctgaca tcactaatgc acatggtgta acgggtgcag gtattgtttc tggcttgaag      180

gaggcagccc aagaaacaac cagtgaacct agaggtttgc taatgcttgc tgagttatca      240

tcaaagggtt ctttagcata tggtgaatat acagaaaaaa cagtagaaat tgctaaatct      300

gataaagagt ttgtcattgg ttttattgcg caacacgata tgggcggtag agaagaaggt      360

tttgactgga tcattatgac tccaggggtt ggtttagatg acaaaggtga tgcacttggt      420

caacaatata gaactgttga tgaagttgta aagactggaa cggatatcat aattgttggt      480

agaggtttgt acggtcaagg aagagatcct atagagcaag ctaaaagata ccaacaagct      540

ggttggaatg cttatttaaa cagatttaaa tgattcttac acaaagattt gatacatgta      600

cactagttta aataagcatg aaaagaatta cacaagcaaa aaaaaaaaaa taaatgaggt      660

actttacgtt cacctacaac caaaaaaact agatagagta aaatcttaag atttagaaaa      720

agttgtttaa caaaggcttt agtatgtgaa tttttaatgt agcaaagcga taactaataa      780

acataaacaa aagtatggtt ttctttatca gtcaaatcat tatcgattga ttgttccgcg      840

tatctgcaga tagcctcatg aaatcagcca tttgcttttg ttcaacgatc ttttgaaatt      900

gttgttgttc ttggtagtta agttgatcca tcttggctta tgttgtgtgt atgttgtagt      960

tattcttagt atattcctgt cctgagttta gtgaaacata atatcgcctt gaaatgaaaa     1020

tgctgaaatt cgtcgacata caatttttca aacttttttt ttttcttggt gcacggacat     1080

gtttttaaag gaagtactct ataccagtta ttcttcacaa atttaattgc tggagaatag     1140

atcttcaacg cgtttaaaca gcaatttgag gaaggaatag gagaaggaga agcaatttct     1200

aggaaagagc aaggtgtgca acagcatgct ctgaatgata ttttcagcaa tagttcagtt     1260

gaagaacctg ttggcgtatc tacatcactt cctacaaaca acaccacgaa ttgcgtccgt     1320

ggtgacgcaa ctacgaatgg cattgtcaat gccaatgcca gtgcacatac acgtgcaagt     1380

cccaccggtt ccctgcccgg ctatggtaga gacaagaagg acgataccgg catcgacatc     1440

aacagtttca acagcaatgc gtttggcgtc gacgcgtcga tggggctgcc gtatttggat     1500

ttggacgggc tagatttcga tatggatatg gatatggata tggatatgga gatgaatttg     1560

aatttagatt tgggtcttga tttggggttg gaattaaaag gggataacaa tgagggtttt     1620

cctgttgatt taaacaatgg acgtgggagg tgattgattt aacctgatcc aaaaggggta     1680

tgtctatttt ttagagtgtg tctttgtgtc aaattatggt agaatgtgta aagtagtata     1740

aactttcctc tcaaatgacg aggtttaaaa caccccccgg gtgagccgag ccgagaatgg     1800

ggcaattgtt caatgtgaaa tagaagtatc gagtgagaaa cttgggtgtt ggccagccaa     1860

gggggaagga aaatggcgcg aatgctcagg tgagattgtt ttggaattgg gtgaagcgag     1920

gaaatgagcg acccggaggt tgtgacttta gtggcggagg aggaacggga ggaaaaggcc     1980

aagagggaaa gtgtatataa gggggagcaa tttgccaacc aggatagaat tggatgagtt     2040

ataattctac tgtatttatt gtataattta tttctccttt tatatcaaac acattacaaa     2100

acacacaaaa catacaaaca tacacagcta gcatgggtga attgaaagag attttgaaac     2160

aaagatatca tgaattactt gattggaatg ttaaggcacc acatgtccct ttatcccaga     2220

gattgaagca ctttacttgg tcatggtttg cttgtactat ggcaaccggt ggtgttggtt     2280

tgatcattgg ttccttccca ttcagattct acggtttgaa caccattggc aagattgttt     2340

acatcttaca aatctttttg ttttctcttt ttggctcttg tatgttgttt cgtttcatca     2400

agtatccatc taccattaag gactcttgga atcatcactt ggaaaagttg tttatcgcaa     2460

cttgtttgtt atctatttcc acattcatcg acatgttagc tatctatgct tatccagata     2520

ccggtgaatg gatggtctgg gtcattagaa tcttatacta catctatgtc gctgtctctt     2580

tcatctactg tgttatggcc tttttcacca ttttcaacaa tcatgtttac actattgaaa     2640

ctgcttctcc agcttggatt ttgccaatct tccctccaat gatctgtggt gtcattgctg     2700

gtgctgttaa ctccacccaa cctgctcacc aattgaaaaa catggtcatt ttcggtatct     2760

tgtttcaagg tttaggtttt tgggtttacc ttttactttt cgccgttaat gttttgagat     2820

tcttcacagt cggtttagca aagccacaag atagaccagg tatgtttatg ttcgttggtc     2880

caccagcttt ctctggttta gcattgatta acattgcaag aggtgcaatg ggctcaagac     2940

cttacatttt cgttggtgca aactcttccg aatacttagg ttttgtctca accttcatgg     3000

ccattttcat ctggggttta gccgcatggt gttattgctt agctatggtt tccttccttg     3060

ccggcttttt cactagagca ccattgaaat tcgcttgtgg ttggttcgct ttcatctttc     3120

caaatgttgg ttttgttaac tgtactatcg aaatcggcaa gatgattgat tctaaggctt     3180

ttcaaatgtt tggtcacatc attggtgtta tcttgtgtat tcaatggatt ttgttaatgt     3240

acttaatggt tagagcattc cttgttaatg acttgtgcta tcctggtaaa gacgaagatg     3300

cacacccacc accaaagcca aacactggtg tcttaaaccc aactttccca ccagagaagg     3360

ctccagcatc attagagaag gttgatactc atgttacatc aacaggtggt gaatccgatc     3420

ctccatcttc cgaacatgaa tccgtttaag gcgcgccatc taatagttta atcacagctt     3480

atagtctact atagttttct tttttaaaca ttgttgtatt ttgtcccccc cctctaattg     3540

atgatgatta tcctataaga atccaataaa acgatggaaa ctaataccct ctcctttgtc     3600

atgtggtctt tagtatttct tgaacattgg ctctgatttc tcgactttat agtcctatta     3660

aaatcgctgt tagttctcga tcgttgtatc tcgtttcttg tctctttggt ggatgatttt     3720

gcgtgcgaac atgttttttt ccctttctct caccatcatc gtgtagttct tgtcaccatc     3780

ccccccaccc cttccttctc tcattgattc tataagagct tatccacaga ggtgcagtaa     3840

cgaggtagtt taaccttcga gtggatcaaa atgtcacaca ggcctgcggc cgcatttggc     3900

aaggcgtatc tatataggag gatcacaaga aaagagagtt caacttaggg aaccaggctt     3960

gacaaaagat aatattaaaa aaggaagaaa aagagagaaa gaaagtaaag acaagaagaa     4020

tcaactccaa tttgaataaa tgggcctgta agaatcttca atctcgatga cggggaaaac     4080

gtcctctttt atagaccaac cccttgtggg gctgtcggga aaagcgggtt tcccgggaaa     4140

ctcatatctg attggggggt tgaagctatt ccgcgttttg gggggtgcgg tttataagaa     4200

gaatgagtaa tgcaaacggc gtttataaag agaaagggtg tggcggtgtt aagcggttga     4260

ggttttaagg tgtgggggac ggggtgtgat tttttcggcg ttacgggcac ggggtatgat     4320

tctcttaatg tcatttccct ttctttctac ttctagggcg cgcagcgtgc gacaatggtg     4380

tttgttgtgc aaataggtgg ttgtgttgtc gttggagttc tcatgaacat atcactctat     4440

tagcaaattt accctaagta tgtaaggttt agtgtgttga ggactaatac agacaatctt     4500

tgtcttatat ataaatggtt ctactcaatt ttataagttt tttttttttt tttttaatct     4560

ctttatgaaa atcaagaatg acattaaaac aatcacaata ttgtatgcaa gaactttgcc     4620

tctaaacctc tttcaaagag atgcataatt ataaaacaaa tctatttgca tattcgcttt     4680

acacaactat tcaagaatat aattacctaa agagtccatc ttgagttcac caacaccact     4740

acttagagct cggtacccgc                                                 4760


<210>  164
<211>  4174
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I. orientalis FUM gene integration fragment

<400>  164
gaattctttg aaggagcttg ccaagaaaca taattttatg atttttgaag atagaaaatt       60

tgctgatatt ggtaacactg ttaaaaatca atataaatct ggtgtcttcc gtattgccga      120

atgggctgac atcactaatg cacatggtgt aacgggtgca ggtattgttt ctggcttgaa      180

ggaggcagcc caagaaacaa ccagtgaacc tagaggtttg ctaatgcttg ctgagttatc      240

atcaaagggt tctttagcat atggtgaata tacagaaaaa acagtagaaa ttgctaaatc      300

tgataaagag tttgtcattg gttttattgc gcaacacgat atgggcggta gagaagaagg      360

ttttgactgg atcattatga ctccaggggt tggtttagat gacaaaggtg atgcacttgg      420

tcaacaatat agaactgttg atgaagttgt aaagactgga acggatatca taattgttgg      480

tagaggtttg tacggtcaag gaagagatcc tatagagcaa gctaaaagat accaacaagc      540

tggttggaat gcttatttaa acagatttaa atgattctta cacaaagatt tgatacatgt      600

acactagttt aaataagcat gaaaagaatt acacaagcaa aaaaaaaaaa ataaatgagg      660

tactttacgt tcacctacaa ccaaaaaaac tagatagagt aaaatcttaa gatttagaaa      720

aagttgttta acaaaggctt tagtatgtga atttttaatg tagcaaagcg ataactaata      780

aacataaaca aaagtatggt tttctttatc agtcaaatca ttatcgattg attgttccgc      840

gtatctgcag atagcctcat gaaatcagcc atttgctttt gttcaacgat cttttgaaat      900

tgttgttgtt cttggtagtt aagttgatcc atcttggctt atgttgtgtg tatgttgtag      960

ttattcttag tatattcctg tcctgagttt agtgaaacat aatatcgcct tgaaatgaaa     1020

atgctgaaat tcgtcgacat acaatttttc aaactttttt tttttcttgg tgcacggaca     1080

tgtttttaaa ggaagtactc tataccagtt attcttcaca aatttaattg ctggagaata     1140

gatcttcaac gcgtttcctc gacatttgct gcaacggcaa catcaatgtc cacgtttaca     1200

cacctacatt tatatctata tttatattta tatttattta tttatgctac ttagcttcta     1260

tagttagtta atgcactcac gatattcaaa attgacaccc ttcaactact ccctactatt     1320

gtctactact gtctactact cctctttact atagctgctc ccaataggct ccaccaatag     1380

gctctgtcaa tacattttgc gccgccacct ttcaggttgt gtcactcctg aaggaccata     1440

ttgggtaatc gtgcaatttc tggaagagag tccgcgagaa gtgaggcccc cactgtaaat     1500

cctcgagggg gcatggagta tggggcatgg aggatggagg atgggggggg ggggggggaa     1560

aataggtagc gaaaggaccc gctatcaccc cacccggaga actcgttgcc gggaagtcat     1620

atttcgacac tccggggagt ctataaaagg cgggttttgt cttttgccag ttgatgttgc     1680

tgagaggact tgtttgccgt ttcttccgat ttaacagtat agaatcaacc actgttaatt     1740

atacacgtta tactaacaca acaaaaacaa aaacaacgac aacaacaaca acatctagat     1800

aaaatgttag ctgctagatc attaaaggca agaatgtcaa caagagcttt ctcaactacc     1860

tcaattgcaa aaagaatcga aaaagatgca tttggtgaca ttgaagtccc aaatgagaaa     1920

tattggggtg ctcaaactca aagatcttta caaaatttca aaattggtgg taagagagaa     1980

gttatgccag aaccaatcat caaatctttt ggtattttaa agaaggctac tgctaagatc     2040

aatgctgagt ctggtgcttt agacccaaag ttatctgaag ccatccaaca agctgcaacc     2100

gaagtttatg aaggtaaact aatggaccat ttcccattag ttgtctttca aaccggttct     2160

ggtactcaat ctaacatgaa tgccaatgaa gtcatctcta atagagcaat tgaaatcttg     2220

ggtggtgaat taggctctaa aactccagtc catcctaatg atcatgttaa tatgtcccaa     2280

tcttctaatg atactttccc tactgtcatg catattgcag cagttacaga agtttcatcc     2340

catttattac cagaattaac tgcactaaga gatgcattgc aaaagaaatc cgatgaattt     2400

aagaatatta tcaaaatcgg tagaacccat ttacaagatg caactccttt aactttaggt     2460

caagaatttt ctggttatgt tcaacaatgt actaatggta tcaaaagaat cgaaattgct     2520

cttgaacatt tgagatactt agctcaaggt ggtactgccg ttggtactgg tcttaacacc     2580

aagaaaggtt ttgctgaaaa ggttgcaaat gaagtcacta aattgactgg tttacaattc     2640

tataccgctc caaataaatt cgaagccctt gcagctcacg atgctgttgt tgaaatgtct     2700

ggtgctttga ataccgttgc agtctcatta ttcaaaatcg ctcaagatat cagatatttg     2760

ggttccggcc caagatgtgg ttatggtgaa ttggctttac cagaaaatga accaggttct     2820

tccatcatgc cgggtaaagt taacccaact caaaacgaag ctttgactat gctttgtacc     2880

caagtctttg gtaaccactc ttgtattacc tttgcaggtg cttcaggtca attcgaattg     2940

aatgtcttta agccagttat gatctccaac ttgttatctt ctattaggtt attaggtgat     3000

ggttgtaatt cttttagaat ccactgtgtt gaaggtatca ttgcaaatac cgacaagatt     3060

gataaattac tacatgaatc tctcatgtta gttactgctt tgaacccaca cattggttac     3120

gataaggctt ccaagattgc aaagaatgca cacaagaagg gcttgacatt gaaacaatct     3180

gcattggaat taggttactt gaccgaagaa caattcaatg aatgggttag accagaaaac     3240

atgattggtc caaaggatta agttaattaa catctgaatg taaaatgaac attaaaatga     3300

attactaaac tttacgtcta ctttacaatc tataaacttt gtttaatcat ataacgaaat     3360

acactaatac acaatcctgt acgtatgtaa tacttttatc catcaaggat tgagaaaaaa     3420

aagtaatgat tccctgggcc attaaaactt agacccccaa gcttggatag gtcactctct     3480

attttcgttt ctcccttccc tgatagaagg gtgatatgta attaagaata atatataatt     3540

ttataataaa agcggccgca ccaggggttt agtgaagtca ccaattaaga ttgttggttt     3600

gagtgagttg ccaaagatct atgaattgat ggagcaaggt aagattttag gcagatatgt     3660

tgttgacact tcgaaatgat gggctgactt gggtgtactg gtgtgacgtt tttatgtgta     3720

tattgatatg catgggggat gtatagtgat gaggagtaga gtatataacg aaatgaaatg     3780

aaataatatg atatgataag ataagatgag atcaatacga taatataaga tgcgacatga     3840

ggagttcaat gtagcatact acacgatgct gcagtacaac tctgatacgc tagactatac     3900

tatacaaaac tgtagtacac tatacgttag tgtagtatcc agaaacaaca ctgctttata     3960

gtacaataca actctataat actatagtat actatgccaa accacgtaat accataatat     4020

gctccacgac atggtacaat gtgctatact tcatactatt ataccatata tactccgata     4080

tattattgat atactatttt atactataat accataccac acaacactac attacaacga     4140

gcaaccttac cataaatgtc agttatggcc gcgg                                 4174


<210>  165
<211>  6874
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FRD gene integration fragment

<400>  165
aaacctccgt tatgtatgtt tgtacccaaa aagaatgcgc tatattagtt taatctttta       60

taaacccgga attataaaaa tacagttagg aataaagtaa tagaaagatg aacaacgggc      120

ctaaaaagac taatgtgttg tggatcggaa tgtttcgaat agagtattaa agttatgctt      180

tcttttcttt ttgaacatgc ttggtattac tttgatatgc aaaagatatc gacaaattga      240

aaatggtttt gatgtctata gatgtggcat ggtaaggttc atttcaattt agcaaatatc      300

agacgagctc agcggccgcg gatccctcga ggagtccatc ggttcctgtc agatgggata      360

ctcttgacgt ggaaaattca aacagaaaaa aaaccccaat aatgaaaaat aacactacgt      420

tatatccgtg gtatcctcta tcgtatcgta tcgtagcgta tcgtagcgta ccgtatcaca      480

gtatagtcta atattccgta tcttattgta tcctatccta ttcgatccta ttgtatttca      540

gtgcaccatt ttaatttcta ttgctataat gtccttatta gttgccactg tgaggtgacc      600

aatggacgag ggcgagccgt tcagaagccg cgaagggtgt tcttcccatg aatttcttaa      660

ggagggcggc tcagctccga gagtgaggcg agacgtctcg gtcagcgtat cccccttcct      720

cggcttttac aaatgatgcg ctcttaatag tgtgtcgtta tccttttggc attgacgggg      780

gagggaaatt gattgagcgc atccatattt ttgcggactg ctgaggacaa tggtggtttt      840

tccgggtggc gtgggctaca aatgatacga tggttttttt cttttcggag aaggcgtata      900

aaaaggacac ggagaaccca tttattctaa aaacagttga gcttctttaa ttattttttg      960

atataatatt ctattattat atattttctt cccaataaaa caaaataaaa caaaacacag     1020

caaaacacaa aaattctaga atggctgatg gcaaaacctc tgcatcagtt gttgctgttg     1080

atgctgaacg tgccgctaag gaaagagatg cagcagctag agctatgttg caaggtggtg     1140

gtgtctctcc tgctggcaag gcacaattgt tgaaaaaggg tttggttcac actgttccat     1200

ataccttaaa ggttgtcgtc gcagatccaa aggaaatgga gaaggcaact gctgacgcag     1260

aagaggtttt acaagctgca tttcaagtcg tcgacaccct tttgaacaac tttaacgaaa     1320

actcagaagt ttcaagagtc aataggttgg cagttggtga ggaacatcaa atgtctgaaa     1380

cattgaaaca cgtcatggcc tgttgtcaaa aggtttatca ttcctccaga ggtgtttttg     1440

acccagcagt tggtccatta gtccgtgaac ttagagaagc tgctcacaag ggtaaaactg     1500

ttccagccga aagagttaat gatttgttat ccaaatgtac ccttaatgca tctttttcaa     1560

ttgatatgtc cagaggtatg attgcaagga agcatccaga cgccatgttg gatttgggtg     1620

gtgtcaacaa gggttatggt atcgactaca ttgttgaaca cttaaactct ttgggttatg     1680

atgatgtctt tttcgaatgg ggtggtgatg ttagagcatc cggcaaaaac cagttatctc     1740

aaccttgggc tgttggtatt gttagaccac ctgccttggc cgacattaga actgttgtcc     1800

cagaggacaa aagatccttt atccgtgtcg tcagattgaa caacgaagct attgctacct     1860

ctggtgatta tgagaatttg gttgaaggtc ctggttctaa ggtttactct tccaccttca     1920

atccaacttc caaaaacttg ttggaaccta ccgaagcagg tatggctcaa gtttctgtca     1980

agtgttgctc atgtatctac gctgatgctt tagcaacagc agctttgttg aaaaacgatc     2040

ctgctgccgt tagaaggatc ttagataact ggagatatgt cagagatact gttactgact     2100

acaccactta cacaagggaa ggtgaaagag ttgctaagat gttggaaatt gctaccgaag     2160

atgctgaaat gagagcaaag agaatcaagg gctctttacc agcaagagtt atcattgttg     2220

gtggtggttt ggccggttgt tccgcagcta tcgaagcagc taactgtggc gcccacgtca     2280

tcttgttaga aaaggaacca aagttaggtg gtaactctgc aaaggctacc tccggtatca     2340

acgcctgggg tactagagca caagcaaaac aaggtgtcat ggacggcggc aagtttttcg     2400

aaagagatac ccatagatcc ggcaagggtg gtaattgcga tccatgcctt gttaagactt     2460

tgtccgttaa gtcctctgat gcagttaagt ggttatctga attaggtgtt ccattgactg     2520

ttttgtctca attaggtggt gcttcaagga aacgttgtca ccgtgcacca gataagtctg     2580

atggtacacc agtcccagtt ggtttcacca ttatgaaaac ccttgaaaac cacattgtca     2640

acgatttgtc cagacatgtt acagttatga caggtattac cgtcacagct ttagaatcta     2700

catcaagagt cagacctgat ggtgttttag tcaagcatgt tactggtgtt cacttgattc     2760

aggcatctgg tcaatctatg gttttgaatg cagacgctgt tatcttagct actggtggtt     2820

tctccaatga tcatacccca aactcccttt tacaacaata cgccccacag ttgtcatctt     2880

ttccaacaac caatggtgtc tgggcaactg gcgatggtgt taagatggct tccaagttgg     2940

gtgtcgcctt agttgatatg gataaggtcc aattacatcc taccggcttg ttagacccaa     3000

aagatccatc taatagaacc aagtatcttg gtccagaggc cttaagaggt tccggcggtg     3060

tcttgttaaa caaaaacggt gaaagatttg ttaatgaatt agacttaaga tctgttgtct     3120

ctcaagctat catcgcacaa gataatgagt acccaggctc tggtggttcc aagttcgcat     3180

actgtgtttt gaacgaaact gcagcaaagt tattcggcaa aaacttcctt ggtttctact     3240

ggaatagatt aggtcttttc caaaaggttg attccgttgc tggtttagct aagttgattg     3300

gttgtccaga agctaatgtt gttgctacat tgaagcaata tgaggagtta tcttccaaaa     3360

agcttaatcc ttgtccattg actggcaagt ctgtctttcc ttgtgtttta ggcactcaag     3420

gtccatacta tgttgccttg gttaccccat ccattcacta cactatgggt ggttgtttga     3480

tttccccatc tgctgagatg caaaccattg acaactctgg tgttactcct gtcagacgtc     3540

caatcttagg cttattcggt gctggtgaag ttactggcgg tgtccatggt ggtaacagat     3600

taggcggtaa ctctttgtta gaatgtgttg ttttcggcaa gatcgctggt gacagagctg     3660

caaccatctt gcaaaagaaa aacaccggct tatcaatgac agaatggtct actgtcgtct     3720

taagagaagt tagagaaggt ggtgtctatg gtgctggttc cagagttttg aggtttaaca     3780

tgcctggtgc attacagaga actggtttag ctttaggtca attcatcggt atcagaggtg     3840

attgggacgg tcacagattg atcggttact attctccaat cactttacct gatgatgttg     3900

gtgttattgg tatcttagct agagcagaca agggtagatt ggcagaatgg atttctgcat     3960

tgcagccagg tgacgctgtt gagatgaagg cctgcggtgg tcttatcatt gacagaagat     4020

tcgctgaaag acatttcttt ttccgtggtc ataagatcag aaagttggcc cttatcggtg     4080

gtggtactgg tgttgcacca atgttacaaa tcgtcagagc tgctgtcaaa aagccatttg     4140

tcgattcaat tgagtccatt cagttcatct atgctgcaga ggatgtttcc gagcttacat     4200

acagaacctt acttgaatct tacgaagagg aatatggttc agaaaagttt aagtgtcact     4260

tcgttttgaa taacccacca gctcaatgga ctgacggtgt tggtttcgtt gatactgcat     4320

tgttgagatc cgcagttcaa gcaccatcaa atgatttgct tgttgcaatt tgtggtccac     4380

caatcatgca aagagcagtt aagggtgcat tgaaaggttt aggttacaat atgaatcttg     4440

ttagaaccgt tgacgaaact gaaccaccat cataattaat taacatctga atgtaaaatg     4500

aacattaaaa tgaattacta aactttacgt ctactttaca atctataaac tttgtttaat     4560

catataacga aatacactaa tacacaatcc tgtacgtatg taatactttt atccatcaag     4620

gattgagaaa aaaaagtaat gattccctgg gccattaaaa cttagacccc caagcttgga     4680

taggtcactc tctattttcg tttctccctt ccctgataga agggtgatat gtaattaaga     4740

ataatatata attttataat aaaagaattc atagcctcat gaaatcagcc atttgctttt     4800

gttcaacgat cttttgaaat tgttgttgtt cttggtagtt aagttgatcc atcttggctt     4860

atgttgtgtg tatgttgtag ttattcttag tatattcctg tcctgagttt agtgaaacat     4920

aatatcgcct tgaaatgaaa atgctgaaat tcgtcgacat acaatttttc aaactttttt     4980

tttttcttgg tgcacggaca tgtttttaaa ggaagtactc tataccagtt attcttcaca     5040

aatttaattg ctggagaata gatcttcaac gctttaataa agtagtttgt ttgtcaagga     5100

tggcgtcata caaagaaaga tcagaatcac acacttcccc tgttgctagg agacttttct     5160

ccatcatgga ggaaaagaag tctaaccttt gtgcatcatt ggatattact gaaactgaaa     5220

agcttctctc tattttggac actattggtc cttacatctg tctagttaaa acacacatcg     5280

atattgtttc tgattttacg tatgaaggaa ctgtgttgcc tttgaaggag cttgccaaga     5340

aacataattt tatgattttt gaagatagaa aatttgctga tattggtaac actgttaaaa     5400

atcaatataa atctggtgtc ttccgtattg ccgaatgggc tgacatcact aatgcacatg     5460

gtgtaacggg tgcaggtatt gtttctggct tgaaggaggc agcccaagaa acaaccagtg     5520

aacctagagg tttgctaatg cttgctgagt tatcatcaaa gggttcttta gcatatggtg     5580

aatatacaga aaaaacagta gaaattgcta aatctgataa agagtttgtc attggtttta     5640

ttgcgcaaca cgatatgggc ggtagagaag aaggttttga ctggatcatt atgactccag     5700

gggttggttt agatgacaaa ggtgatgcac ttggtcaaca atatagaact gttgatgaag     5760

ttgtaaagac tggaacggat atcataattg ttggtagagg tttgtacggt caaggaagag     5820

atcctataga gcaagctaaa agataccaac aagctggttg gaatgcttat ttaaacagat     5880

ttaaatgatt cttacacaaa gatttgatac atgtacacta gtttaaataa gcatgaaaag     5940

aattacacaa gcaaaaaaaa aaaaataaat gaggtacttt acgttcacct acaaccaaaa     6000

aaactagata gagtaaaatc ttaagattta gaaaaagttg tttaacaaag gctttagtat     6060

gtgaattttt aatgtagcaa agcgataact aataaacata aacaaaagta tggttttctt     6120

tatcagtcaa atcattatcg attgattgtt ccgcgtatct gcagatagcc tcatgaaatc     6180

agccatttgc ttttgttcaa cgatcttttg aaattgttgt tgttcttggt agttaagttg     6240

atccatcttg gcttatgttg tgtgtatgtt gtagttattc ttagtatatt cctgtcctga     6300

gtttagtgaa acataatatc gccttgaaat gaaaatgctg aaattcgtcg acatacaatt     6360

tttcaaactt tttttttttc ttggtgcacg gacatgtttt taaaggaagt actctatacc     6420

agttattctt cacaaattta attgctggag aatagatctt caacgccccg ggggatctgg     6480

atccgcggcc gctcatatgt ttgaaggtat tatcactgct gttgatttac gttcttgaaa     6540

actgcacgga taatattcac aatactaaca ataaagaaga ctcattgtgg aaggtgactc     6600

aatcatgcta gaaaagctgg ggaataaagg cacttttata gtagccacat tttggttcaa     6660

aagaatataa aggaaaaaaa aatattttcc agtgaaaaag aaaagactct ttctccgaga     6720

agccgagttt ctacgaggcc ttgttgagtc ataggggacc tctgtggttg actccggctt     6780

attacgtgaa tcatcggggg agccgcaccg tttgtccgcg acaggagaaa acgcaaggag     6840

tcaaacatta aattggtagg cactaccgag gttt                                 6874


<210>  166
<211>  987
<212>  DNA
<213>  Rhizopus delemar


<220>
<221>  CDS
<222>  (1)..(987)

<400>  166
atg gtt aaa gtt aca gtt tgt ggt gct gct ggt ggt att ggt caa ccc         48
Met Val Lys Val Thr Val Cys Gly Ala Ala Gly Gly Ile Gly Gln Pro           
1               5                   10                  15                

ctt tct tta ctc ttg aag caa tcc tct cac att act cac tta tct ctt         96
Leu Ser Leu Leu Leu Lys Gln Ser Ser His Ile Thr His Leu Ser Leu           
            20                  25                  30                    

tat gat atc gtt aat act cct ggt gtt gct gct gat ctt agt cat atc        144
Tyr Asp Ile Val Asn Thr Pro Gly Val Ala Ala Asp Leu Ser His Ile           
        35                  40                  45                        

gat acc aaa tcc aag gtc act ggt cat gta ggt gct gct caa ctt gaa        192
Asp Thr Lys Ser Lys Val Thr Gly His Val Gly Ala Ala Gln Leu Glu           
    50                  55                  60                            

gaa gct atc aag gat tct gat gtt gtc gtt att ccc gct ggt gtc cca        240
Glu Ala Ile Lys Asp Ser Asp Val Val Val Ile Pro Ala Gly Val Pro           
65                  70                  75                  80            

aga aag cca ggt atg acg cgt gat gat ctt ttc aag att aat gct ggt        288
Arg Lys Pro Gly Met Thr Arg Asp Asp Leu Phe Lys Ile Asn Ala Gly           
                85                  90                  95                

att gta cgt gat ttg gct aca gct gct gca aag tac gct cca aag gcc        336
Ile Val Arg Asp Leu Ala Thr Ala Ala Ala Lys Tyr Ala Pro Lys Ala           
            100                 105                 110                   

ttc atg tgt atc att tct aac cca gtc aac tcg act gtc cca atc gtt        384
Phe Met Cys Ile Ile Ser Asn Pro Val Asn Ser Thr Val Pro Ile Val           
        115                 120                 125                       

act gaa gta ttc aaa cag cac aat gtt tat gac ccc aaa aga atc ttt        432
Thr Glu Val Phe Lys Gln His Asn Val Tyr Asp Pro Lys Arg Ile Phe           
    130                 135                 140                           

ggt gtt aca aca ctt gat att gtt cgt gca tcc acc ttt gta tcc gaa        480
Gly Val Thr Thr Leu Asp Ile Val Arg Ala Ser Thr Phe Val Ser Glu           
145                 150                 155                 160           

ttg att gga ggt gaa cct aat tca ctt cgt gtt ccc gtc att ggt ggt        528
Leu Ile Gly Gly Glu Pro Asn Ser Leu Arg Val Pro Val Ile Gly Gly           
                165                 170                 175               

cac agc ggc gta acc atc tta cct tta ctc tca cag gtc ccc ggc att        576
His Ser Gly Val Thr Ile Leu Pro Leu Leu Ser Gln Val Pro Gly Ile           
            180                 185                 190                   

gaa aag tta aac caa gaa caa att gag aag gta act cat cgt att caa        624
Glu Lys Leu Asn Gln Glu Gln Ile Glu Lys Val Thr His Arg Ile Gln           
        195                 200                 205                       

ttt ggt ggc gat gaa gtt gtc aag gcc aag gat ggt gct ggt tct gcc        672
Phe Gly Gly Asp Glu Val Val Lys Ala Lys Asp Gly Ala Gly Ser Ala           
    210                 215                 220                           

act ctt tcc atg gct tat gct ggt gct cgt ttt gct aca aac atc att        720
Thr Leu Ser Met Ala Tyr Ala Gly Ala Arg Phe Ala Thr Asn Ile Ile           
225                 230                 235                 240           

gag gct gct ttt gct gga aag aag ggc att gtt gaa tgt acc tat gtt        768
Glu Ala Ala Phe Ala Gly Lys Lys Gly Ile Val Glu Cys Thr Tyr Val           
                245                 250                 255               

caa ttg gat gct gat aaa tct ggt gcc caa tct gtc aag gat ttg gtt        816
Gln Leu Asp Ala Asp Lys Ser Gly Ala Gln Ser Val Lys Asp Leu Val           
            260                 265                 270                   

ggt agt gaa ctt gaa tat ttc tct gtt ccc gtt gaa ttg ggt cct agt        864
Gly Ser Glu Leu Glu Tyr Phe Ser Val Pro Val Glu Leu Gly Pro Ser           
        275                 280                 285                       

ggt gtt gaa aag att tta ccc att gga aac gtt aat gaa tat gaa aag        912
Gly Val Glu Lys Ile Leu Pro Ile Gly Asn Val Asn Glu Tyr Glu Lys           
    290                 295                 300                           

aag ttg ttg aac gag gct tct cct gaa tta aaa acc aac att gat aaa        960
Lys Leu Leu Asn Glu Ala Ser Pro Glu Leu Lys Thr Asn Ile Asp Lys           
305                 310                 315                 320           

ggt tgt act ttt gtt act gaa ggc taa                                    987
Gly Cys Thr Phe Val Thr Glu Gly                                           
                325                                                       


<210>  167
<211>  328
<212>  PRT
<213>  Rhizopus delemar

<400>  167

Met Val Lys Val Thr Val Cys Gly Ala Ala Gly Gly Ile Gly Gln Pro 
1               5                   10                  15      


Leu Ser Leu Leu Leu Lys Gln Ser Ser His Ile Thr His Leu Ser Leu 
            20                  25                  30          


Tyr Asp Ile Val Asn Thr Pro Gly Val Ala Ala Asp Leu Ser His Ile 
        35                  40                  45              


Asp Thr Lys Ser Lys Val Thr Gly His Val Gly Ala Ala Gln Leu Glu 
    50                  55                  60                  


Glu Ala Ile Lys Asp Ser Asp Val Val Val Ile Pro Ala Gly Val Pro 
65                  70                  75                  80  


Arg Lys Pro Gly Met Thr Arg Asp Asp Leu Phe Lys Ile Asn Ala Gly 
                85                  90                  95      


Ile Val Arg Asp Leu Ala Thr Ala Ala Ala Lys Tyr Ala Pro Lys Ala 
            100                 105                 110         


Phe Met Cys Ile Ile Ser Asn Pro Val Asn Ser Thr Val Pro Ile Val 
        115                 120                 125             


Thr Glu Val Phe Lys Gln His Asn Val Tyr Asp Pro Lys Arg Ile Phe 
    130                 135                 140                 


Gly Val Thr Thr Leu Asp Ile Val Arg Ala Ser Thr Phe Val Ser Glu 
145                 150                 155                 160 


Leu Ile Gly Gly Glu Pro Asn Ser Leu Arg Val Pro Val Ile Gly Gly 
                165                 170                 175     


His Ser Gly Val Thr Ile Leu Pro Leu Leu Ser Gln Val Pro Gly Ile 
            180                 185                 190         


Glu Lys Leu Asn Gln Glu Gln Ile Glu Lys Val Thr His Arg Ile Gln 
        195                 200                 205             


Phe Gly Gly Asp Glu Val Val Lys Ala Lys Asp Gly Ala Gly Ser Ala 
    210                 215                 220                 


Thr Leu Ser Met Ala Tyr Ala Gly Ala Arg Phe Ala Thr Asn Ile Ile 
225                 230                 235                 240 


Glu Ala Ala Phe Ala Gly Lys Lys Gly Ile Val Glu Cys Thr Tyr Val 
                245                 250                 255     


Gln Leu Asp Ala Asp Lys Ser Gly Ala Gln Ser Val Lys Asp Leu Val 
            260                 265                 270         


Gly Ser Glu Leu Glu Tyr Phe Ser Val Pro Val Glu Leu Gly Pro Ser 
        275                 280                 285             


Gly Val Glu Lys Ile Leu Pro Ile Gly Asn Val Asn Glu Tyr Glu Lys 
    290                 295                 300                 


Lys Leu Leu Asn Glu Ala Ser Pro Glu Leu Lys Thr Asn Ile Asp Lys 
305                 310                 315                 320 


Gly Cys Thr Phe Val Thr Glu Gly 
                325             


<210>  168
<211>  4127
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MDH gene integration fragment

<400>  168
gttaacccgt ttcgatggga ttcccagaag tggatactat actgtctgca atgcactaca       60

ctctaaaaaa gtattataca ttaccataca ttagcaaatc accaatactc tgcactgttt      120

cagtgtgtgc acattgctac ccaattggga aatcgcaggg aaaatgagac accccctcca      180

ttcgtattac gtaagacaat atcagggctg ccgaattcgg cagaaaagcc gagccggccg      240

agtcctcttg cacggagtgt gtccgaaaag ggcagctctg cagtggggga gaggaggtcg      300

cacgtctatg cggtgttggc atggcctgtg cgtgtacctg tcccctccct gggcatcccc      360

cactgcgcgc cttctccatt gggcgctgcg ggcactccgc gccgttaata caggaggggg      420

gggggaaagc ttaagattag agcgggtaca gtcagtgggt gtattgaccc catttctgtc      480

agtataaacc ccccgttgag ccgccggttt ggttgtttat ggataaaatt tttttttccc      540

cgcatggaga agattgaggg ggggaaggaa tgggaaaaag gccagagcca tctccacagc      600

ggaatccgac cgttaatggg gtgaaacacc cccaccaggt agagcaggaa gaatggggaa      660

acaaggtgga gagatggtca ttgttgggaa tagtgggaaa atgaggggga agagaatgac      720

tataaaatgg gaagggggtc caagttatcc aagcagtcca tttagagaag ggagcggccc      780

ctattggtag ttctttcccc ctctcaagct ggcgtgaaat gcaaccttac ggcgtctacg      840

ttactacaag gtccagaaag tgtaggtatt gctactattt ttatttttta ttggttctgg      900

agaaatgcag acagtcaatg aacacaactg tctcaatatg catctatgca catgcacaca      960

cacacacatc acaggtaccc ctacaaagag aggtctcttg ataatgtttc attaccacgt     1020

ggcatccccc cccccccccc caataaacaa gtggccgagt tcccctgttg cagaggagga     1080

caaaaaaacc gctggtgttg gtaccattat gcagcaacta gcacaacaaa caaccgaccc     1140

agacatacaa atcaacaaca cttcgccaaa gacacccttt ccagggagga tccactccca     1200

acgtctctcc ataatgtctc tgttggccca tgtctctgtc gttgacaccg taaccacacc     1260

aaccaacccg tccattgtac tgggatggtc gtccatagac acctctccaa cggggaacac     1320

ctcattcgta aaccgccaag gttaccgttc ctcctgactc gccccgttgt tgatgctgcg     1380

cacctgtggt tgcccaacat ggttgtatat cgtgtaacca caccaacaca tgtgcagcac     1440

atgtgtttaa aagagtgtca tggaggtgga tcatgatgga agtggacttt accacttggg     1500

aactgtctcc actcccggga agaaaagacc cggcgtatca cgcggttgcc tcaatggggc     1560

aatttggaag gagaaatata gggaaaatca cgtcgctctc ggacggggaa gagttccaga     1620

ctatgagggg ggggggtggt atataaagac aggagatgtc cacccccaga gagaggaaga     1680

agttggaact ttagaagaga gagataactt tccccagtgt ccatcaatac acaaccaaac     1740

acaaactcta tatttacaca tataaccccc tctctagaat ggttaaagtt acagtttgtg     1800

gtgctgctgg tggtattggt caaccccttt ctttactctt gaagcaatcc tctcacatta     1860

ctcacttatc tctttatgat atcgttaata ctcctggtgt tgctgctgat cttagtcata     1920

tcgataccaa atccaaggtc actggtcatg taggtgctgc tcaacttgaa gaagctatca     1980

aggattctga tgttgtcgtt attcccgctg gtgtcccaag aaagccaggt atgacgcgtg     2040

atgatctttt caagattaat gctggtattg tacgtgattt ggctacagct gctgcaaagt     2100

acgctccaaa ggccttcatg tgtatcattt ctaacccagt caactcgact gtcccaatcg     2160

ttactgaagt attcaaacag cacaatgttt atgaccccaa aagaatcttt ggtgtaacaa     2220

cacttgatat tgttcgtgca tccacctttg tatccgaatt gattggaggt gaacctaatt     2280

cacttcgtgt tcccgtcatt ggtggtcaca gcggcgtaac catcttacct ttactctcac     2340

aggtccccgg cattgaaaag ttaaaccaag aacaaattga gaaggtaact catcgtattc     2400

aatttggtgg cgatgaagtt gtcaaggcca aggatggtgc tggttctgcc actctttcca     2460

tggcttatgc tggtgctcgt tttgctacaa acatcattga ggctgctttt gctggaaaga     2520

agggcattgt tgaatgtacc tatgttcaat tggatgctga taaatctggt gcccaatctg     2580

tcaaggattt ggttggtagt gaacttgaat atttctctgt tcccgttgaa ttgggtccta     2640

gtggtgttga aaagatttta cccattggaa acgttaatga atatgaaaag aagttgttga     2700

acgaggcttc tcctgaatta aaaaccaaca ttgataaagg ttgtactttt gttactgaag     2760

gctcaaagtt gtaattaatt aatttatttt actagtttat ttttgctcct gagaatagga     2820

ttacaaacac ttaaagtctt taattacaac tatatataat attctgttgg ttttcttgaa     2880

ttggttcgct gcgattcatg cctcccattc accaaaggtg gagtgggaaa taacggtttt     2940

actgcggtaa ttagcagagg caagaacagg atacactttt tgatgataaa tctgtattat     3000

agtcgagcct atttaggaaa tcaaattttc ttgtgtttac ttttcaaata aataatgttc     3060

gaaaattttt actttactcc ttcatttaac tataccagac gttatatcat caacaccttc     3120

tgaccatata cagctcaaga tgtttaagag tctgttaaat tttttcaatc catttcatgg     3180

agtaccagga ggtgctacaa aaggaattca tagcctcatg aaatcagcca tttgcttttg     3240

ttcaacgatc ttttgaaatt gttgttgttc ttggtagtta agttgatcca tcttggctta     3300

tgttgtgtgt atgttgtagt tattcttagt atattcctgt cctgagttta gtgaaacata     3360

atatcgcctt gaaatgaaaa tgctgaaatt cgtcgacata caatttttca aacttttttt     3420

ttttcttggt gcacggacat gtttttaaag gaagtactct ataccagtta ttcttcacaa     3480

atttaattgc tggagaatag atcttcaacg ctttaataaa gtagtttgtt tgtcaaggat     3540

ggcgtcatac aaagaaagat cagaatcaca cacttcccct gttgctagga gacttttctc     3600

catcatggag gaaaagaagt ctaacctttg tgcatcattg gatattactg aaactgaaaa     3660

gcttctctct attttggaca ctattggtcc ttacatctgt ctagttaaaa cacacatcga     3720

tattgtttct gattttacgt atgaaggaac tgtgttgcct ttgaaggagc ttgccaagaa     3780

acataatttt atgatttttg aagatagaaa atttgctgat attggtaaca ctgttaaaaa     3840

tcaatataaa tctggtgtct tccgtattgc cgaatgggct gacatcacta atgcacatgg     3900

tgtaacgggt gcaggtattg tttctggctt gaaggaggca gcccaagaaa caaccagtga     3960

acctagaggt ttgctaatgc ttgctgagtt atcatcaaag ggttctttag catatggtga     4020

atatacagaa aaaacagtag aaattgctaa atctgataaa gagtttgtca ttggttttat     4080

tgcgcaacac gatatgggcg gtagagaaga aggttttgac tccgcgg                   4127


<210>  169
<211>  6874
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FRD gene integration fragment

<400>  169
aaacctcggt agtgcctacc aatttaatgt ttgactcctt gcgttttctc ctgtcgcgga       60

caaacggtgc ggctcccccg atgattcacg taataagccg gagtcaacca cagaggtccc      120

ctatgactca acaaggcctc gtagaaactc ggcttctcgg agaaagagtc ttttcttttt      180

cactggaaaa tatttttttt tcctttatat tcttttgaac caaaatgtgg ctactataaa      240

agtgccttta ttccccagct tttctagcat gattgagtca ccttccacaa tgagtcttct      300

ttattgttag tattgtgaat attatccgtg cagttttcaa gaacgtaaat caacagcagt      360

gataatacct tcaaacatat gagcggccgc ggatccctcg aggagtccat cggttcctgt      420

cagatgggat actcttgacg tggaaaattc aaacagaaaa aaaaccccaa taatgaaaaa      480

taacactacg ttatatccgt ggtatcctct atcgtatcgt atcgtagcgt atcgtagcgt      540

accgtatcac agtatagtct aatattccgt atcttattgt atcctatcct attcgatcct      600

attgtatttc agtgcaccat tttaatttct attgctataa tgtccttatt agttgccact      660

gtgaggtgac caatggacga gggcgagccg ttcagaagcc gcgaagggtg ttcttcccat      720

gaatttctta aggagggcgg ctcagctccg agagtgaggc gagacgtctc ggtcagcgta      780

tcccccttcc tcggctttta caaatgatgc gctcttaata gtgtgtcgtt atccttttgg      840

cattgacggg ggagggaaat tgattgagcg catccatatt tttgcggact gctgaggaca      900

atggtggttt ttccgggtgg cgtgggctac aaatgatacg atggtttttt tcttttcgga      960

gaaggcgtat aaaaaggaca cggagaaccc atttattcta aaaacagttg agcttcttta     1020

attatttttt gatataatat tctattatta tatattttct tcccaataaa acaaaataaa     1080

acaaaacaca gcaaaacaca aaaattctag aatggctgat ggcaaaacct ctgcatcagt     1140

tgttgctgtt gatgctgaac gtgccgctaa ggaaagagat gcagcagcta gagctatgtt     1200

gcaaggtggt ggtgtctctc ctgctggcaa ggcacaattg ttgaaaaagg gtttggttca     1260

cactgttcca tataccttaa aggttgtcgt cgcagatcca aaggaaatgg agaaggcaac     1320

tgctgacgca gaagaggttt tacaagctgc atttcaagtc gtcgacaccc ttttgaacaa     1380

ctttaacgaa aactcagaag tttcaagagt caataggttg gcagttggtg aggaacatca     1440

aatgtctgaa acattgaaac acgtcatggc ctgttgtcaa aaggtttatc attcctccag     1500

aggtgttttt gacccagcag ttggtccatt agtccgtgaa cttagagaag ctgctcacaa     1560

gggtaaaact gttccagccg aaagagttaa tgatttgtta tccaaatgta cccttaatgc     1620

atctttttca attgatatgt ccagaggtat gattgcaagg aagcatccag acgccatgtt     1680

ggatttgggt ggtgtcaaca agggttatgg tatcgactac attgttgaac acttaaactc     1740

tttgggttat gatgatgtct ttttcgaatg gggtggtgat gttagagcat ccggcaaaaa     1800

ccagttatct caaccttggg ctgttggtat tgttagacca cctgccttgg ccgacattag     1860

aactgttgtc ccagaggaca aaagatcctt tatccgtgtc gtcagattga acaacgaagc     1920

tattgctacc tctggtgatt atgagaattt ggttgaaggt cctggttcta aggtttactc     1980

ttccaccttc aatccaactt ccaaaaactt gttggaacct accgaagcag gtatggctca     2040

agtttctgtc aagtgttgct catgtatcta cgctgatgct ttagcaacag cagctttgtt     2100

gaaaaacgat cctgctgccg ttagaaggat cttagataac tggagatatg tcagagatac     2160

tgttactgac tacaccactt acacaaggga aggtgaaaga gttgctaaga tgttggaaat     2220

tgctaccgaa gatgctgaaa tgagagcaaa gagaatcaag ggctctttac cagcaagagt     2280

tatcattgtt ggtggtggtt tggccggttg ttccgcagct atcgaagcag ctaactgtgg     2340

cgcccacgtc atcttgttag aaaaggaacc aaagttaggt ggtaactctg caaaggctac     2400

ctccggtatc aacgcctggg gtactagagc acaagcaaaa caaggtgtca tggacggcgg     2460

caagtttttc gaaagagata cccatagatc cggcaagggt ggtaattgcg atccatgcct     2520

tgttaagact ttgtccgtta agtcctctga tgcagttaag tggttatctg aattaggtgt     2580

tccattgact gttttgtctc aattaggtgg tgcttcaagg aaacgttgtc accgtgcacc     2640

agataagtct gatggtacac cagtcccagt tggtttcacc attatgaaaa cccttgaaaa     2700

ccacattgtc aacgatttgt ccagacatgt tacagttatg acaggtatta ccgtcacagc     2760

tttagaatct acatcaagag tcagacctga tggtgtttta gtcaagcatg ttactggtgt     2820

tcacttgatt caggcatctg gtcaatctat ggttttgaat gcagacgctg ttatcttagc     2880

tactggtggt ttctccaatg atcatacccc aaactccctt ttacaacaat acgccccaca     2940

gttgtcatct tttccaacaa ccaatggtgt ctgggcaact ggcgatggtg ttaagatggc     3000

ttccaagttg ggtgtcgcct tagttgatat ggataaggtc caattacatc ctaccggctt     3060

gttagaccca aaagatccat ctaatagaac caagtatctt ggtccagagg ccttaagagg     3120

ttccggcggt gtcttgttaa acaaaaacgg tgaaagattt gttaatgaat tagacttaag     3180

atctgttgtc tctcaagcta tcatcgcaca agataatgag tacccaggct ctggtggttc     3240

caagttcgca tactgtgttt tgaacgaaac tgcagcaaag ttattcggca aaaacttcct     3300

tggtttctac tggaatagat taggtctttt ccaaaaggtt gattccgttg ctggtttagc     3360

taagttgatt ggttgtccag aagctaatgt tgttgctaca ttgaagcaat atgaggagtt     3420

atcttccaaa aagcttaatc cttgtccatt gactggcaag tctgtctttc cttgtgtttt     3480

aggcactcaa ggtccatact atgttgcctt ggttacccca tccattcact acactatggg     3540

tggttgtttg atttccccat ctgctgagat gcaaaccatt gacaactctg gtgttactcc     3600

tgtcagacgt ccaatcttag gcttattcgg tgctggtgaa gttactggcg gtgtccatgg     3660

tggtaacaga ttaggcggta actctttgtt agaatgtgtt gttttcggca agatcgctgg     3720

tgacagagct gcaaccatct tgcaaaagaa aaacaccggc ttatcaatga cagaatggtc     3780

tactgtcgtc ttaagagaag ttagagaagg tggtgtctat ggtgctggtt ccagagtttt     3840

gaggtttaac atgcctggtg cattacagag aactggttta gctttaggtc aattcatcgg     3900

tatcagaggt gattgggacg gtcacagatt gatcggttac tattctccaa tcactttacc     3960

tgatgatgtt ggtgttattg gtatcttagc tagagcagac aagggtagat tggcagaatg     4020

gatttctgca ttgcagccag gtgacgctgt tgagatgaag gcctgcggtg gtcttatcat     4080

tgacagaaga ttcgctgaaa gacatttctt tttccgtggt cataagatca gaaagttggc     4140

ccttatcggt ggtggtactg gtgttgcacc aatgttacaa atcgtcagag ctgctgtcaa     4200

aaagccattt gtcgattcaa ttgagtccat tcagttcatc tatgctgcag aggatgtttc     4260

cgagcttaca tacagaacct tacttgaatc ttacgaagag gaatatggtt cagaaaagtt     4320

taagtgtcac ttcgttttga ataacccacc agctcaatgg actgacggtg ttggtttcgt     4380

tgatactgca ttgttgagat ccgcagttca agcaccatca aatgatttgc ttgttgcaat     4440

ttgtggtcca ccaatcatgc aaagagcagt taagggtgca ttgaaaggtt taggttacaa     4500

tatgaatctt gttagaaccg ttgacgaaac tgaaccacca tcataattaa ttaacatctg     4560

aatgtaaaat gaacattaaa atgaattact aaactttacg tctactttac aatctataaa     4620

ctttgtttaa tcatataacg aaatacacta atacacaatc ctgtacgtat gtaatacttt     4680

tatccatcaa ggattgagaa aaaaaagtaa tgattccctg ggccattaaa acttagaccc     4740

ccaagcttgg ataggtcact ctctattttc gtttctccct tccctgatag aagggtgata     4800

tgtaattaag aataatatat aattttataa taaaagaatt catagcctca tgaaatcagc     4860

catttgcttt tgttcaacga tcttttgaaa ttgttgttgt tcttggtagt taagttgatc     4920

catcttggct tatgttgtgt gtatgttgta gttattctta gtatattcct gtcctgagtt     4980

tagtgaaaca taatatcgcc ttgaaatgaa aatgctgaaa ttcgtcgaca tacaattttt     5040

caaacttttt ttttttcttg gtgcacggac atgtttttaa aggaagtact ctataccagt     5100

tattcttcac aaatttaatt gctggagaat agatcttcaa cgctttaata aagtagtttg     5160

tttgtcaagg atggcgtcat acaaagaaag atcagaatca cacacttccc ctgttgctag     5220

gagacttttc tccatcatgg aggaaaagaa gtctaacctt tgtgcatcat tggatattac     5280

tgaaactgaa aagcttctct ctattttgga cactattggt ccttacatct gtctagttaa     5340

aacacacatc gatattgttt ctgattttac gtatgaagga actgtgttgc ctttgaagga     5400

gcttgccaag aaacataatt ttatgatttt tgaagataga aaatttgctg atattggtaa     5460

cactgttaaa aatcaatata aatctggtgt cttccgtatt gccgaatggg ctgacatcac     5520

taatgcacat ggtgtaacgg gtgcaggtat tgtttctggc ttgaaggagg cagcccaaga     5580

aacaaccagt gaacctagag gtttgctaat gcttgctgag ttatcatcaa agggttcttt     5640

agcatatggt gaatatacag aaaaaacagt agaaattgct aaatctgata aagagtttgt     5700

cattggtttt attgcgcaac acgatatggg cggtagagaa gaaggttttg actggatcat     5760

tatgactcca ggggttggtt tagatgacaa aggtgatgca cttggtcaac aatatagaac     5820

tgttgatgaa gttgtaaaga ctggaacgga tatcataatt gttggtagag gtttgtacgg     5880

tcaaggaaga gatcctatag agcaagctaa aagataccaa caagctggtt ggaatgctta     5940

tttaaacaga tttaaatgat tcttacacaa agatttgata catgtacact agtttaaata     6000

agcatgaaaa gaattacaca agcaaaaaaa aaaaaataaa tgaggtactt tacgttcacc     6060

tacaaccaaa aaaactagat agagtaaaat cttaagattt agaaaaagtt gtttaacaaa     6120

ggctttagta tgtgaatttt taatgtagca aagcgataac taataaacat aaacaaaagt     6180

atggttttct ttatcagtca aatcattatc gattgattgt tccgcgtatc tgcagatagc     6240

ctcatgaaat cagccatttg cttttgttca acgatctttt gaaattgttg ttgttcttgg     6300

tagttaagtt gatccatctt ggcttatgtt gtgtgtatgt tgtagttatt cttagtatat     6360

tcctgtcctg agtttagtga aacataatat cgccttgaaa tgaaaatgct gaaattcgtc     6420

gacatacaat ttttcaaact tttttttttt cttggtgcac ggacatgttt ttaaaggaag     6480

tactctatac cagttattct tcacaaattt aattgctgga gaatagatct tcaacgcccc     6540

gggggatctg gatccgcggc cgctgagctc gtctgatatt tgctaaattg aaatgaacct     6600

taccatgcca catctataga catcaaaacc attttcaatt tgtcgatatc ttttgcatat     6660

caaagtaata ccaagcatgt tcaaaaagaa aagaaagcat aactttaata ctctattcga     6720

aacattccga tccacaacac attagtcttt ttaggcccgt tgttcatctt tctattactt     6780

tattcctaac tgtattttta taattccggg tttataaaag attaaactaa tatagcgcat     6840

tctttttggg tacaaacata cataacggag gttt                                 6874


