                         SEQUENCE LISTING

<110>  Case Western Reserve University
 
<120>  GLUTAMIC ACID-STABILIZED INSULIN ANALOGUES

<130>  200512.00220

<150>  61/589,271
<151>  2012-01-20

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  86
<212>  PRT
<213>  Homo sapiens

<400>  1

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Arg Arg 
            20                  25                  30          


Glu Ala Glu Asp Leu Gln Val Gly Gln Val Glu Leu Gly Gly Gly Pro 
        35                  40                  45              


Gly Ala Gly Ser Leu Gln Pro Leu Ala Leu Glu Gly Ser Leu Gln Lys 
    50                  55                  60                  


Arg Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln 
65                  70                  75                  80  


Leu Glu Asn Tyr Cys Asn 
                85      


<210>  2
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  2

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  3
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  3

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
            20                  25                  30  


<210>  4
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  4

Gly Ile Val Glu Gln Cys Cys Glu Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  5
<211>  32
<212>  PRT
<213>  Homo sapiens

<400>  5

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Glu Glu 
            20                  25                  30          


<210>  6
<211>  32
<212>  PRT
<213>  Homo sapiens

<400>  6

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Lys Pro Thr Glu Glu 
            20                  25                  30          


<210>  7
<211>  32
<212>  PRT
<213>  Homo sapiens

<400>  7

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Asp Pro Thr Glu Glu 
            20                  25                  30          


<210>  8
<211>  32
<212>  PRT
<213>  Homo sapiens

<400>  8

Phe Val Lys Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Glu Thr Glu Glu 
            20                  25                  30          


<210>  9
<211>  31
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Asn or Lys

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  His, Asp or Glu

<220>
<221>  MISC_FEATURE
<222>  (24)..(24)
<223>  Cyclohexanylalanine, penta fluoro Phe, ortho-monofluoro-Phe, 
       ortho-monochloro-Phe, ortho-monobromo-Phe, para-monofluoro-Phe, 
       para -monochloro-Phe, para -monobromo-Phe

<220>
<221>  MISC_FEATURE
<222>  (24)..(24)
<223>  Cyclohexanylalanine, penta fluoro Phe, ortho-monofluoro-Phe, 
       ortho-monochloro-Phe, ortho-monobromo-Phe, para-monofluoro-Phe, 
       para -monochloro-Phe, para -monobromo-Phe

<220>
<221>  MISC_FEATURE
<222>  (27)..(27)
<223>  Asp, Pro, Lys, or Arg

<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Lys, Pro, or Ala

<400>  9

Phe Val Xaa Gln His Leu Cys Gly Ser Xaa Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Xaa Phe Thr Xaa Xaa Thr Glu Glu 
            20                  25                  30      


<210>  10
<211>  31
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  His, Asp or Glu

<220>
<221>  MISC_FEATURE
<222>  (23)..(23)
<223>  Cyclohexanylalanine, penta fluoro Phe, ortho-monofluoro-Phe, 
       ortho-monochloro-Phe, ortho-monobromo-Phe, para-monofluoro-Phe, 
       para -monochloro-Phe, para -monobromo-Phe

<220>
<221>  MISC_FEATURE
<222>  (23)..(23)
<223>  Cyclohexanylalanine, penta fluoro Phe, ortho-monofluoro-Phe, 
       ortho-monochloro-Phe, ortho-monobromo-Phe, para-monofluoro-Phe, 
       para -monochloro-Phe, para -monobromo-Phe

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (27)..(27)
<223>  Asp, Pro, Lys, or Arg

<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Ornithine, Diaminobutyric acid, Diaminoproprionic acid, 
       Norleucine, Aminobutric acid, or Aminoproprionic acid

<400>  10

Phe Val Asn Gln His Leu Cys Gly Ser Xaa Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Xaa Phe Thr Xaa Xaa Thr Glu Glu 
            20                  25                  30      


<210>  11
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  11

Gly Phe Phe Tyr Thr Pro Lys Thr Glu Glu 
1               5                   10  


<210>  12
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  12

Gly Phe Phe Tyr Thr Lys Pro Thr Glu Glu 
1               5                   10  


<210>  13
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  13

Gly Phe Phe Tyr Thr Asp Lys Thr Glu Glu 
1               5                   10  


<210>  14
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  14

Gly Phe Phe Tyr Thr Pro Glu Thr Glu Glu 
1               5                   10  


<210>  15
<211>  10
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Cyclohexanylalanine, penta fluoro Phe, ortho-monofluoro-Phe, 
       ortho-monochloro-Phe, ortho-monobromo-Phe, para-monofluoro-Phe, 
       para -monochloro-Phe, para -monobromo-Phe

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Cyclohexanylalanine, penta fluoro Phe, ortho-monofluoro-Phe, 
       ortho-monochloro-Phe, ortho-monobromo-Phe, para-monofluoro-Phe, 
       para -monochloro-Phe, para -monobromo-Phe

<400>  15

Gly Xaa Phe Tyr Thr Asp Lys Thr Glu Glu 
1               5                   10  


<210>  16
<211>  10
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Cyclohexanylalanine, penta fluoro Phe, ortho-monofluoro-Phe, 
       ortho-monochloro-Phe, ortho-monobromo-Phe, para-monofluoro-Phe, 
       para -monochloro-Phe, para -monobromo-Phe; and Leu, Lys or Asp

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Cyclohexanylalanine, penta fluoro Phe, ortho-monofluoro-Phe, 
       ortho-monochloro-Phe, ortho-monobromo-Phe, para-monofluoro-Phe, 
       para -monochloro-Phe, para -monobromo-Phe; and  Leu, Lys or Asp

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Xaa can be any naturally occurring amino acid

<400>  16

Gly Xaa Phe Tyr Thr Xaa Pro Thr Glu Glu 
1               5                   10  


<210>  17
<211>  96
<212>  DNA
<213>  Homo sapiens

<400>  17
tttgtgaacc aacacctgtg cggctcacac ctggtggaag ctctctacct agtgtgcggg       60

gaacgaggct agttctacac acccaagacc gaagaa                                 96


<210>  18
<211>  96
<212>  DNA
<213>  Homo sapiens

<400>  18
tttgttaacc aacatttgtg tggttctcat ttggttgaag ctttgtactt ggtttgtggt       60

gaaagaggtt agttttacac tccaaagact gaagaa                                 96


