                         SEQUENCE LISTING

<110>  VIB VZW
       UNIVERSITEIT GENT
       VRIJE UNIVERSITEIT BRUSSEL
 
<120>  Tools and methods for expression of membrane proteins

<130>  JS-NC/CO/404

<150>  EP 12151814.6
<151>  2012-01-19

<150>  US 61/588,523
<151>  2012-01-19

<160>  31    

<170>  PatentIn version 3.5

<210>  1
<211>  129
<212>  PRT
<213>  Lama glama

<400>  1

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Ser Ile Asn 
            20                  25                  30          


Ala Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Ala Ile Thr Ser Gly Gly Ser Thr Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Glu Asn Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr Ser Cys Asn 
                85                  90                  95      


Ala Glu Gly Thr Ser Gly Ser Ser Arg Tyr Arg Arg Arg Tyr Glu Tyr 
            100                 105                 110         


Trp Gly Lys Gly Thr Gln Val Thr Val Ser Ser His His His His His 
        115                 120                 125             


His 
    


<210>  2
<211>  126
<212>  PRT
<213>  Lama glama

<400>  2

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Arg Thr Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Gly Ser Gly Ser Phe Phe Ser Ile Asn 
            20                  25                  30          


Pro Met Gly Trp Tyr Arg Gln Ala Pro Gly Gln Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Thr Ile Thr Gly Ser Gly Ser Thr Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Ala Gly Tyr Phe Asp Arg Ile Gly Arg Arg Tyr Asp Arg Trp Gly Gln 
            100                 105                 110         


Gly Thr Gln Val Thr Val Ser Ser His His His His His His 
        115                 120                 125     


<210>  3
<211>  130
<212>  PRT
<213>  Lama glama

<400>  3

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Thr Ile Asn Ser Gly Gly Arg Ser Ala Asn Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Leu Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu His 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Arg Ser Val Ser Arg Asn Tyr Val Pro Leu Gly Tyr Asp 
            100                 105                 110         


Tyr Leu Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His His 
        115                 120                 125             


His His 
    130 


<210>  4
<211>  125
<212>  PRT
<213>  Lama glama

<400>  4

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Thr Ser Gly Ile Ile 
            20                  25                  30          


Ala Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Arg Ile Ser Ser Gly Ser Ser Thr Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Val Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Ala Val Arg Arg Gly Tyr Arg Asn Asp Tyr Asn Ser Trp Gly Gln Gly 
            100                 105                 110         


Thr Gln Val Thr Val Ser Ser His His His His His His 
        115                 120                 125 


<210>  5
<211>  130
<212>  PRT
<213>  Lama glama

<400>  5

Met Glu Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15      


Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Ser Ile 
            20                  25                  30          


Asn Thr Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu 
        35                  40                  45              


Val Ala Ala Ile His Ser Gly Gly Ser Thr Asn Tyr Ala Asn Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Ala Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Asn Val Lys Asp Tyr Gly Ala Val Leu Tyr Glu Tyr Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser Ala Ala Ala His His His His 
        115                 120                 125             


His His 
    130 


<210>  6
<211>  136
<212>  PRT
<213>  Lama glama

<400>  6

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Phe Arg Ser Ile Val 
            20                  25                  30          


Ser Met Ala Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Ser Ser Asn Ser Gly Gly Ser Thr Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Trp Cys Asn 
                85                  90                  95      


Val Gln Asn Arg Leu Pro Gly Phe Asp Ala Phe Ser Gly Arg Ser Ile 
            100                 105                 110         


Ala Glu Thr Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Ala 
        115                 120                 125             


Ala Ala His His His His His His 
    130                 135     


<210>  7
<211>  8
<212>  PRT
<213>  Lama glama

<400>  7

Gly Ser Ile Phe Ser Ile Asn Ala 
1               5               


<210>  8
<211>  16
<212>  PRT
<213>  Lama glama

<400>  8

Ala Ala Ile Thr Ser Gly Gly Ser Thr Asn Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


<210>  9
<211>  17
<212>  PRT
<213>  Lama glama

<400>  9

Asn Ala Glu Gly Thr Ser Gly Ser Ser Arg Tyr Arg Arg Arg Tyr Glu 
1               5                   10                  15      


Tyr 
    


<210>  10
<211>  8
<212>  PRT
<213>  Lama glama

<400>  10

Gly Ser Phe Phe Ser Ile Asn Pro 
1               5               


<210>  11
<211>  16
<212>  PRT
<213>  Lama glama

<400>  11

Ala Thr Ile Thr Gly Ser Gly Ser Thr Asn Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


<210>  12
<211>  14
<212>  PRT
<213>  Lama glama

<400>  12

Asn Ala Gly Tyr Phe Asp Arg Ile Gly Arg Arg Tyr Asp Arg 
1               5                   10                  


<210>  13
<211>  8
<212>  PRT
<213>  Lama glama

<400>  13

Gly Phe Thr Phe Ser Ser Tyr Ala 
1               5               


<210>  14
<211>  15
<212>  PRT
<213>  Lama glama

<400>  14

Ile Asn Ser Gly Gly Arg Ser Ala Asn Tyr Ala Asp Ser Val Lys 
1               5                   10                  15  


<210>  15
<211>  17
<212>  PRT
<213>  Lama glama

<400>  15

Ala Arg Pro Arg Ser Val Ser Arg Asn Tyr Val Pro Leu Gly Tyr Asp 
1               5                   10                  15      


Tyr 
    


<210>  16
<211>  8
<212>  PRT
<213>  Lama glama

<400>  16

Gly Ser Thr Ser Gly Ile Ile Ala 
1               5               


<210>  17
<211>  16
<212>  PRT
<213>  Lama glama

<400>  17

Ala Arg Ile Ser Ser Gly Ser Ser Thr Asn Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


<210>  18
<211>  13
<212>  PRT
<213>  Lama glama

<400>  18

Asn Ala Val Arg Arg Gly Tyr Arg Asn Asp Tyr Asn Ser 
1               5                   10              


<210>  19
<211>  387
<212>  DNA
<213>  Lama glama

<400>  19
caggtgcagc tgcaggagtc tgggggaggc ttggtgcagc ctggggggtc tctgagactc       60

tcctgtgcag cctctggaag catcttcagt atcaatgcca tgggctggta ccgccaggct      120

ccagggaagc agcgcgagtt ggtcgcagct attactagtg gtggtagcac aaactatgca      180

gactccgtaa agggccgatt caccatctcc agagacaacg ccgagaacac ggtgtatctg      240

caaatgaaca acctgaaacc tgaggacacg gccgtctatt catgtaacgc tgaaggaacg      300

tcgggtagta gccggtatcg ccgccggtat gagtactggg gcaaggggac ccaggtcacc      360

gtctcctcac accaccatca ccatcac                                          387


<210>  20
<211>  378
<212>  DNA
<213>  Lama glama

<400>  20
caggtgcagc tgcaggagtc tggaggaggc ttggtgcgca ctggggggtc tctgagactc       60

tcctgtgcag gctctggaag cttcttcagt atcaatccca tgggctggta ccgccaggct      120

ccagggcagc agcgcgagtt ggtcgcaact attactggta gtggtagcac aaactatgca      180

gactccgtga agggccgatt caccatctcc agagacaacg ccaagaacac actgtatctg      240

caaatgaaca gcctgaaacc tgaggacacg gccgtctatt actgtaatgc aggatatttc      300

gatcggattg gtcggcggta tgaccgctgg ggccagggga cccaggtcac cgtctcctca      360

caccaccatc accatcac                                                    378


<210>  21
<211>  393
<212>  DNA
<213>  Lama glama

<400>  21
caggtgcagc tgcaggagtc tggaggaggc ttggtgcagc ctggggggtc tctgagactc       60

tcctgtgcag cctctggatt caccttcagt agctatgcca tgagctgggt ccgccaggct      120

ccaggaaagg ggctcgagtg ggtctcaact attaatagtg gtggtcgtag cgcaaactat      180

gcagactccg tgaagggccg actcaccatc tccagagaca acgccaagaa cacgctgcat      240

ctgcaaatga acagcctgaa acctgaggac acggccctgt attactgtgc gagaccccgt      300

agtgtaagtc gcaactatgt tccactcgga tacgactact tgggccaggg gacccaggtc      360

accgtctcct cacaccacca tcaccatcac tag                                   393


<210>  22
<211>  384
<212>  DNA
<213>  Lama glama

<400>  22
caggtgcagc tgcaggagtc tggaggaggc ttggtgcagg ctggggggtc tctgagactc       60

tcctgtgcag cctctggaag cacctccggt atcattgcca tgggctggta ccgccaggct      120

ccagggaagc agcgcgagtt ggtcgcacgt attagtagtg gtagtagtac aaactatgca      180

gactccgtga agggccgatt caccgtctcc agagacaacg ccaagaacac agtgtatctg      240

caaatgaaca gcctgaaacc tgaggacacg gccgtctatt actgtaatgc agtccgtcga      300

ggttaccgta acgactataa ctcctggggc caggggaccc aggtcaccgt ctcctcacac      360

caccatcacc atcactagac tagt                                             384


<210>  23
<211>  393
<212>  DNA
<213>  Lama glama

<400>  23
atggaggtgc agctgcagga gtctggggga ggcttggtgc aggctggggg gtctctgaga       60

ctctcctgtg cagcctctgg aagcatcttc agtatcaata ccatgggctg gtaccgccag      120

gctccaggga agcagcgcga gttggtcgca gctattcata gtggtggtag cacaaactat      180

gccaactccg tgaagggccg attcaccatc tccagagaca atgccgcgaa cacggtgtat      240

ctgcaaatga acagcctgaa acctgaggac acggccgtct attactgtaa tgtaaaggac      300

tacggggcgg tcctatatga gtatgactac tggggccagg ggacccaggt caccgtctcc      360

tcagcggccg cacatcatca tcaccatcac tag                                   393


<210>  24
<211>  408
<212>  DNA
<213>  Lama glama

<400>  24
caggtgcagc tggtggagtc tgggggaggc ttggtgcagc ctggggggtc tctgagactc       60

tcctgtgcag cctctggaag cttccgcagt atcgtgtcta tggcctggta ccgccaggct      120

ccagggaagc agcgcgagtt ggtcgcaagt tctaatagtg ggggcagcac aaattatgca      180

gactccgtga agggccgatt caccatctcc agagacaacg ccaagaacac ggtgtatctg      240

caaatgaaca gcctgaaacc tgaggacacg gccgtgtatt ggtgtaatgt ccaaaaccgc      300

ctcccgggat tcgacgcctt tagtggcaga tctatagcgg agacctattg gggccagggg      360

acccaggtca ccgtctcctc agcggccgca catcatcatc accatcac                   408


<210>  25
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminal Rho1D4-tag

<400>  25

Thr Glu Thr Ser Gln Val Ala Pro Ala 
1               5                   


<210>  26
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  26
gaagaagggg tatctctcga gaaaaggcag gtgcagctgc agg                         43


<210>  27
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  27
tcatgtctaa ggctaactag tctagtgatg gtgatggtgg                             40


<210>  28
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  28
gactggttcc aattgacaag c                                                 21


<210>  29
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  29
gcaaatggca ttctgacatc c                                                 21


<210>  30
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  30
agtctgctcg agccaccatg gaggtgcagc tgcagg                                 36


<210>  31
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  31
cagactagat ctctagtgat ggtgatgatg atgtgcggcc gctgaggaga cggtgacctg       60

ggtcc                                                                   65


