                         SEQUENCE LISTING

<110>  VIB VZW
       UNIVERSITEIT GENT
       CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
       UNIVERSIT MONTPELLIER 2
       CENTRE HOSPITALIER REGIONAL UNIVERSITAIRE DE MONTPELLIER
       UNIVERSITT OSNABRCK
 
<120>  TARGETED MUTANT ALPHA-HELICAL BUNDLE CYTOKINES

<130>  JT/TARG/403

<150>  EP 12305075.9
<151>  2012-01-20

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  1
ggggggtccg gaccatcacc atcaccatca ccatcaccat caccctgctt ctcccgcctc       60

cccagcatca cctgccagcc cagcaagtga tagcctggaa tttattgc                   108


<210>  2
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  2
cgtctagatc attccttact tcttaaac                                          28


<210>  3
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  3
cacgactgta ggccccagcg a                                                 21


<210>  4
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  4
agcagctgtc tctcccctcc g                                                 21


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  5
agagggaaat cgtgcgtgac                                                   20


<210>  6
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  6
caatagtgat gacctggccg t                                                 21


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  7
gagctagagc ctgcagcaat                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  8
ttctgggcaa tctgcttctt                                                   20


<210>  9
<211>  352
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H6-Leptin construct


<220>
<221>  MISC_FEATURE
<222>  (94)..(94)
<223>  Xaa can be Ser or Asn

<400>  9

His His His His His His Gly Gly Ser Gly Ile Gln Lys Val Gln Asp 
1               5                   10                  15      


Asp Thr Lys Thr Leu Ile Lys Thr Ile Val Thr Arg Ile Asn Asp Ile 
            20                  25                  30          


Ser His Thr Gln Ser Val Ser Ala Lys Gln Arg Val Thr Gly Leu Asp 
        35                  40                  45              


Phe Ile Pro Gly Leu His Pro Ile Leu Ser Leu Ser Lys Met Asp Gln 
    50                  55                  60                  


Thr Leu Ala Val Tyr Gln Gln Val Leu Thr Ser Leu Pro Ser Gln Asn 
65                  70                  75                  80  


Val Leu Gln Ile Ala Asn Asp Leu Glu Asn Leu Arg Asp Xaa Leu His 
                85                  90                  95      


Leu Leu Ala Phe Ser Lys Ser Cys Ser Leu Pro Gln Thr Ser Gly Leu 
            100                 105                 110         


Gln Lys Pro Glu Ser Leu Asp Gly Val Leu Glu Ala Ser Leu Tyr Ser 
        115                 120                 125             


Thr Glu Val Val Ala Leu Ser Arg Leu Gln Gly Ser Leu Gln Asp Ile 
    130                 135                 140                 


Leu Gln Gln Leu Asp Val Ser Pro Glu Cys Ala Leu Asp Gly Gly Ser 
145                 150                 155                 160 


Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
                165                 170                 175     


Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
            180                 185                 190         


Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
        195                 200                 205             


Gly Gly Ser Gly Gly Ser Gly Gly Ser Ser Gly Ser Met Ala Gln Val 
    210                 215                 220                 


Gln Leu Gln Glu Ser Gly Gly Gly Val Val Gln Pro Gly Gly Ser Leu 
225                 230                 235                 240 


Thr Leu Ser Cys Thr Arg Thr Gly Phe Thr Ala Ser Thr Asn Ala Val 
                245                 250                 255     


Gly Trp Tyr Arg Gln Gly Pro Gly Lys Lys Cys Glu Trp Val Ser Tyr 
            260                 265                 270         


Met Thr Ile Pro Ser Gly Arg Thr Thr Tyr Ala Asp Ala Val Lys Gly 
        275                 280                 285             


Arg Phe Ala Met Ser Arg Asp Lys Ala Lys Ser Thr Val Phe Leu Gln 
    290                 295                 300                 


Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Gly Asp 
305                 310                 315                 320 


Val Pro Phe Ser Thr Leu Pro Ala Met Cys Thr Asn Asp Gly Pro Trp 
                325                 330                 335     


Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His His His His 
            340                 345                 350         


<210>  10
<211>  345
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mleptin construct


<220>
<221>  MISC_FEATURE
<222>  (88)..(88)
<223>  Xaa can be Ser or Asn

<400>  10

Gly Gly Ser Gly Ile Gln Lys Val Gln Asp Asp Thr Lys Thr Leu Ile 
1               5                   10                  15      


Lys Thr Ile Val Thr Arg Ile Asn Asp Ile Ser His Thr Gln Ser Val 
            20                  25                  30          


Ser Ala Lys Gln Arg Val Thr Gly Leu Asp Phe Ile Pro Gly Leu His 
        35                  40                  45              


Pro Ile Leu Ser Leu Ser Lys Met Asp Gln Thr Leu Ala Val Tyr Gln 
    50                  55                  60                  


Gln Val Leu Thr Ser Leu Pro Ser Gln Asn Val Leu Gln Ile Ala Asn 
65                  70                  75                  80  


Asp Leu Glu Asn Leu Arg Asp Xaa Leu His Leu Leu Ala Phe Ser Lys 
                85                  90                  95      


Ser Cys Ser Leu Pro Gln Thr Ser Gly Leu Gln Lys Pro Glu Ser Leu 
            100                 105                 110         


Asp Gly Val Leu Glu Ala Ser Leu Tyr Ser Thr Glu Val Val Ala Leu 
        115                 120                 125             


Ser Arg Leu Gln Gly Ser Leu Gln Asp Ile Leu Gln Gln Leu Asp Val 
    130                 135                 140                 


Ser Pro Glu Cys Ala Leu Asp Gly Gly Ser Gly Gly Ser Gly Gly Ser 
145                 150                 155                 160 


Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
                165                 170                 175     


Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
            180                 185                 190         


Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
        195                 200                 205             


Gly Gly Ser Ser Gly Ser Met Ala Gln Val Gln Leu Gln Glu Ser Gly 
    210                 215                 220                 


Gly Gly Val Val Gln Pro Gly Gly Ser Leu Thr Leu Ser Cys Thr Arg 
225                 230                 235                 240 


Thr Gly Phe Thr Ala Ser Thr Asn Ala Val Gly Trp Tyr Arg Gln Gly 
                245                 250                 255     


Pro Gly Lys Lys Cys Glu Trp Val Ser Tyr Met Thr Ile Pro Ser Gly 
            260                 265                 270         


Arg Thr Thr Tyr Ala Asp Ala Val Lys Gly Arg Phe Ala Met Ser Arg 
        275                 280                 285             


Asp Lys Ala Lys Ser Thr Val Phe Leu Gln Asn Ser Leu Lys Pro Glu 
    290                 295                 300                 


Asp Thr Ala Val Tyr Tyr Cys Gly Asp Val Pro Phe Ser Thr Leu Pro 
305                 310                 315                 320 


Ala Met Cys Thr Asn Asp Gly Pro Trp Gly Gln Gly Thr Gln Val Thr 
                325                 330                 335     


Val Ser Ser His His His His His His 
            340                 345 


