                         SEQUENCE LISTING

<110>  Ventana Medical Systems, Inc.
 
<120>  AUTOMATED ANALYSIS OF CIRCULATING TUMOUR CELLS

<130>  7668-88299-03

<150>  61/581,825
<151>  2011-12-30

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  1461
<212>  DNA
<213>  Homo sapiens


<220>
<221>  CDS
<222>  (1)..(1461)

<400>  1
atg att cag act gtc ccg gac cca gca gct cat atc aag gaa gcc tta         48
Met Ile Gln Thr Val Pro Asp Pro Ala Ala His Ile Lys Glu Ala Leu           
1               5                   10                  15                

tca gtt gtg agt gag gac cag tcg ttg ttt gag tgt gcc tac gga acg         96
Ser Val Val Ser Glu Asp Gln Ser Leu Phe Glu Cys Ala Tyr Gly Thr           
            20                  25                  30                    

cca cac ctg gct aag aca gag atg acc gcg tcc tcc tcc agc gac tat        144
Pro His Leu Ala Lys Thr Glu Met Thr Ala Ser Ser Ser Ser Asp Tyr           
        35                  40                  45                        

gga cag act tcc aag atg agc cca cgc gtc cct cag cag gat tgg ctg        192
Gly Gln Thr Ser Lys Met Ser Pro Arg Val Pro Gln Gln Asp Trp Leu           
    50                  55                  60                            

tct caa ccc cca gcc agg gtc acc atc aaa atg gaa tgt aac cct agc        240
Ser Gln Pro Pro Ala Arg Val Thr Ile Lys Met Glu Cys Asn Pro Ser           
65                  70                  75                  80            

cag gtg aat ggc tca agg aac tct cct gat gaa tgc agt gtg gcc aaa        288
Gln Val Asn Gly Ser Arg Asn Ser Pro Asp Glu Cys Ser Val Ala Lys           
                85                  90                  95                

ggc ggg aag atg gtg ggc agc cca gac acc gtt ggg atg aac tac ggc        336
Gly Gly Lys Met Val Gly Ser Pro Asp Thr Val Gly Met Asn Tyr Gly           
            100                 105                 110                   

agc tac atg gag gag aag cac atg cca ccc cca aac atg acc acg aac        384
Ser Tyr Met Glu Glu Lys His Met Pro Pro Pro Asn Met Thr Thr Asn           
        115                 120                 125                       

gag cgc aga gtt atc gtg cca gca gat cct acg cta tgg agt aca gac        432
Glu Arg Arg Val Ile Val Pro Ala Asp Pro Thr Leu Trp Ser Thr Asp           
    130                 135                 140                           

cat gtg cgg cag tgg ctg gag tgg gcg gtg aaa gaa tat ggc ctt cca        480
His Val Arg Gln Trp Leu Glu Trp Ala Val Lys Glu Tyr Gly Leu Pro           
145                 150                 155                 160           

gac gtc aac atc ttg tta ttc cag aac atc gat ggg aag gaa ctg tgc        528
Asp Val Asn Ile Leu Leu Phe Gln Asn Ile Asp Gly Lys Glu Leu Cys           
                165                 170                 175               

aag atg acc aag gac gac ttc cag agg ctc acc ccc agc tac aac gcc        576
Lys Met Thr Lys Asp Asp Phe Gln Arg Leu Thr Pro Ser Tyr Asn Ala           
            180                 185                 190                   

gac atc ctt ctc tca cat ctc cac tac ctc aga gag act cct ctt cca        624
Asp Ile Leu Leu Ser His Leu His Tyr Leu Arg Glu Thr Pro Leu Pro           
        195                 200                 205                       

cat ttg act tca gat gat gtt gat aaa gcc tta caa aac tct cca cgg        672
His Leu Thr Ser Asp Asp Val Asp Lys Ala Leu Gln Asn Ser Pro Arg           
    210                 215                 220                           

tta atg cat gct aga aac aca ggg ggt gca gct ttt att ttc cca aat        720
Leu Met His Ala Arg Asn Thr Gly Gly Ala Ala Phe Ile Phe Pro Asn           
225                 230                 235                 240           

act tca gta tat cct gaa gct acg caa aga att aca act agg cca gat        768
Thr Ser Val Tyr Pro Glu Ala Thr Gln Arg Ile Thr Thr Arg Pro Asp           
                245                 250                 255               

tta cca tat gag ccc ccc agg aga tca gcc tgg acc ggt cac ggc cac        816
Leu Pro Tyr Glu Pro Pro Arg Arg Ser Ala Trp Thr Gly His Gly His           
            260                 265                 270                   

ccc acg ccc cag tcg aaa gct gct caa cca tct cct tcc aca gtg ccc        864
Pro Thr Pro Gln Ser Lys Ala Ala Gln Pro Ser Pro Ser Thr Val Pro           
        275                 280                 285                       

aaa act gaa gac cag cgt cct cag tta gat cct tat cag att ctt gga        912
Lys Thr Glu Asp Gln Arg Pro Gln Leu Asp Pro Tyr Gln Ile Leu Gly           
    290                 295                 300                           

cca aca agt agc cgc ctt gca aat cca ggc agt ggc cag atc cag ctt        960
Pro Thr Ser Ser Arg Leu Ala Asn Pro Gly Ser Gly Gln Ile Gln Leu           
305                 310                 315                 320           

tgg cag ttc ctc ctg gag ctc ctg tcg gac agc tcc aac tcc agc tgc       1008
Trp Gln Phe Leu Leu Glu Leu Leu Ser Asp Ser Ser Asn Ser Ser Cys           
                325                 330                 335               

atc acc tgg gaa ggc acc aac ggg gag ttc aag atg acg gat ccc gac       1056
Ile Thr Trp Glu Gly Thr Asn Gly Glu Phe Lys Met Thr Asp Pro Asp           
            340                 345                 350                   

gag gtg gcc cgg cgc tgg gga gag cgg aag agc aaa ccc aac atg aac       1104
Glu Val Ala Arg Arg Trp Gly Glu Arg Lys Ser Lys Pro Asn Met Asn           
        355                 360                 365                       

tac gat aag ctc agc cgc gcc ctc cgt tac tac tat gac aag aac atc       1152
Tyr Asp Lys Leu Ser Arg Ala Leu Arg Tyr Tyr Tyr Asp Lys Asn Ile           
    370                 375                 380                           

atg acc aag gtc cat ggg aag cgc tac gcc tac aag ttc gac ttc cac       1200
Met Thr Lys Val His Gly Lys Arg Tyr Ala Tyr Lys Phe Asp Phe His           
385                 390                 395                 400           

ggg atc gcc cag gcc ctc cag ccc cac ccc ccg gag tca tct ctg tac       1248
Gly Ile Ala Gln Ala Leu Gln Pro His Pro Pro Glu Ser Ser Leu Tyr           
                405                 410                 415               

aag tac ccc tca gac ctc ccg tac atg ggc tcc tat cac gcc cac cca       1296
Lys Tyr Pro Ser Asp Leu Pro Tyr Met Gly Ser Tyr His Ala His Pro           
            420                 425                 430                   

cag aag atg aac ttt gtg gcg ccc cac cct cca gcc ctc ccc gtg aca       1344
Gln Lys Met Asn Phe Val Ala Pro His Pro Pro Ala Leu Pro Val Thr           
        435                 440                 445                       

tct tcc agt ttt ttt gct gcc cca aac cca tac tgg aat tca cca act       1392
Ser Ser Ser Phe Phe Ala Ala Pro Asn Pro Tyr Trp Asn Ser Pro Thr           
    450                 455                 460                           

ggg ggt ata tac ccc aac act agg ctc ccc acc agc cat atg cct tct       1440
Gly Gly Ile Tyr Pro Asn Thr Arg Leu Pro Thr Ser His Met Pro Ser           
465                 470                 475                 480           

cat ctg ggc act tac tac taa                                           1461
His Leu Gly Thr Tyr Tyr                                                   
                485                                                       


<210>  2
<211>  486
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ile Gln Thr Val Pro Asp Pro Ala Ala His Ile Lys Glu Ala Leu 
1               5                   10                  15      


Ser Val Val Ser Glu Asp Gln Ser Leu Phe Glu Cys Ala Tyr Gly Thr 
            20                  25                  30          


Pro His Leu Ala Lys Thr Glu Met Thr Ala Ser Ser Ser Ser Asp Tyr 
        35                  40                  45              


Gly Gln Thr Ser Lys Met Ser Pro Arg Val Pro Gln Gln Asp Trp Leu 
    50                  55                  60                  


Ser Gln Pro Pro Ala Arg Val Thr Ile Lys Met Glu Cys Asn Pro Ser 
65                  70                  75                  80  


Gln Val Asn Gly Ser Arg Asn Ser Pro Asp Glu Cys Ser Val Ala Lys 
                85                  90                  95      


Gly Gly Lys Met Val Gly Ser Pro Asp Thr Val Gly Met Asn Tyr Gly 
            100                 105                 110         


Ser Tyr Met Glu Glu Lys His Met Pro Pro Pro Asn Met Thr Thr Asn 
        115                 120                 125             


Glu Arg Arg Val Ile Val Pro Ala Asp Pro Thr Leu Trp Ser Thr Asp 
    130                 135                 140                 


His Val Arg Gln Trp Leu Glu Trp Ala Val Lys Glu Tyr Gly Leu Pro 
145                 150                 155                 160 


Asp Val Asn Ile Leu Leu Phe Gln Asn Ile Asp Gly Lys Glu Leu Cys 
                165                 170                 175     


Lys Met Thr Lys Asp Asp Phe Gln Arg Leu Thr Pro Ser Tyr Asn Ala 
            180                 185                 190         


Asp Ile Leu Leu Ser His Leu His Tyr Leu Arg Glu Thr Pro Leu Pro 
        195                 200                 205             


His Leu Thr Ser Asp Asp Val Asp Lys Ala Leu Gln Asn Ser Pro Arg 
    210                 215                 220                 


Leu Met His Ala Arg Asn Thr Gly Gly Ala Ala Phe Ile Phe Pro Asn 
225                 230                 235                 240 


Thr Ser Val Tyr Pro Glu Ala Thr Gln Arg Ile Thr Thr Arg Pro Asp 
                245                 250                 255     


Leu Pro Tyr Glu Pro Pro Arg Arg Ser Ala Trp Thr Gly His Gly His 
            260                 265                 270         


Pro Thr Pro Gln Ser Lys Ala Ala Gln Pro Ser Pro Ser Thr Val Pro 
        275                 280                 285             


Lys Thr Glu Asp Gln Arg Pro Gln Leu Asp Pro Tyr Gln Ile Leu Gly 
    290                 295                 300                 


Pro Thr Ser Ser Arg Leu Ala Asn Pro Gly Ser Gly Gln Ile Gln Leu 
305                 310                 315                 320 


Trp Gln Phe Leu Leu Glu Leu Leu Ser Asp Ser Ser Asn Ser Ser Cys 
                325                 330                 335     


Ile Thr Trp Glu Gly Thr Asn Gly Glu Phe Lys Met Thr Asp Pro Asp 
            340                 345                 350         


Glu Val Ala Arg Arg Trp Gly Glu Arg Lys Ser Lys Pro Asn Met Asn 
        355                 360                 365             


Tyr Asp Lys Leu Ser Arg Ala Leu Arg Tyr Tyr Tyr Asp Lys Asn Ile 
    370                 375                 380                 


Met Thr Lys Val His Gly Lys Arg Tyr Ala Tyr Lys Phe Asp Phe His 
385                 390                 395                 400 


Gly Ile Ala Gln Ala Leu Gln Pro His Pro Pro Glu Ser Ser Leu Tyr 
                405                 410                 415     


Lys Tyr Pro Ser Asp Leu Pro Tyr Met Gly Ser Tyr His Ala His Pro 
            420                 425                 430         


Gln Lys Met Asn Phe Val Ala Pro His Pro Pro Ala Leu Pro Val Thr 
        435                 440                 445             


Ser Ser Ser Phe Phe Ala Ala Pro Asn Pro Tyr Trp Asn Ser Pro Thr 
    450                 455                 460                 


Gly Gly Ile Tyr Pro Asn Thr Arg Leu Pro Thr Ser His Met Pro Ser 
465                 470                 475                 480 


His Leu Gly Thr Tyr Tyr 
                485     


<210>  3
<211>  1212
<212>  DNA
<213>  Homo sapiens


<220>
<221>  CDS
<222>  (1)..(1212)

<400>  3
atg aca gcc atc atc aaa gag atc gtt agc aga aac aaa agg aga tat         48
Met Thr Ala Ile Ile Lys Glu Ile Val Ser Arg Asn Lys Arg Arg Tyr           
1               5                   10                  15                

caa gag gat gga ttc gac tta gac ttg acc tat att tat cca aac att         96
Gln Glu Asp Gly Phe Asp Leu Asp Leu Thr Tyr Ile Tyr Pro Asn Ile           
            20                  25                  30                    

att gct atg gga ttt cct gca gaa aga ctt gaa ggc gta tac agg aac        144
Ile Ala Met Gly Phe Pro Ala Glu Arg Leu Glu Gly Val Tyr Arg Asn           
        35                  40                  45                        

aat att gat gat gta gta agg ttt ttg gat tca aag cat aaa aac cat        192
Asn Ile Asp Asp Val Val Arg Phe Leu Asp Ser Lys His Lys Asn His           
    50                  55                  60                            

tac aag ata tac aat ctt tgt gct gaa aga cat tat gac acc gcc aaa        240
Tyr Lys Ile Tyr Asn Leu Cys Ala Glu Arg His Tyr Asp Thr Ala Lys           
65                  70                  75                  80            

ttt aat tgc aga gtt gca caa tat cct ttt gaa gac cat aac cca cca        288
Phe Asn Cys Arg Val Ala Gln Tyr Pro Phe Glu Asp His Asn Pro Pro           
                85                  90                  95                

cag cta gaa ctt atc aaa ccc ttt tgt gaa gat ctt gac caa tgg cta        336
Gln Leu Glu Leu Ile Lys Pro Phe Cys Glu Asp Leu Asp Gln Trp Leu           
            100                 105                 110                   

agt gaa gat gac aat cat gtt gca gca att cac tgt aaa gct gga aag        384
Ser Glu Asp Asp Asn His Val Ala Ala Ile His Cys Lys Ala Gly Lys           
        115                 120                 125                       

gga cga act ggt gta atg ata tgt gca tat tta tta cat cgg ggc aaa        432
Gly Arg Thr Gly Val Met Ile Cys Ala Tyr Leu Leu His Arg Gly Lys           
    130                 135                 140                           

ttt tta aag gca caa gag gcc cta gat ttc tat ggg gaa gta agg acc        480
Phe Leu Lys Ala Gln Glu Ala Leu Asp Phe Tyr Gly Glu Val Arg Thr           
145                 150                 155                 160           

aga gac aaa aag gga gta act att ccc agt cag agg cgc tat gtg tat        528
Arg Asp Lys Lys Gly Val Thr Ile Pro Ser Gln Arg Arg Tyr Val Tyr           
                165                 170                 175               

tat tat agc tac ctg tta aag aat cat ctg gat tat aga cca gtg gca        576
Tyr Tyr Ser Tyr Leu Leu Lys Asn His Leu Asp Tyr Arg Pro Val Ala           
            180                 185                 190                   

ctg ttg ttt cac aag atg atg ttt gaa act att cca atg ttc agt ggc        624
Leu Leu Phe His Lys Met Met Phe Glu Thr Ile Pro Met Phe Ser Gly           
        195                 200                 205                       

gga act tgc aat cct cag ttt gtg gtc tgc cag cta aag gtg aag ata        672
Gly Thr Cys Asn Pro Gln Phe Val Val Cys Gln Leu Lys Val Lys Ile           
    210                 215                 220                           

tat tcc tcc aat tca gga ccc aca cga cgg gaa gac aag ttc atg tac        720
Tyr Ser Ser Asn Ser Gly Pro Thr Arg Arg Glu Asp Lys Phe Met Tyr           
225                 230                 235                 240           

ttt gag ttc cct cag ccg tta cct gtg tgt ggt gat atc aaa gta gag        768
Phe Glu Phe Pro Gln Pro Leu Pro Val Cys Gly Asp Ile Lys Val Glu           
                245                 250                 255               

ttc ttc cac aaa cag aac aag atg cta aaa aag gac aaa atg ttt cac        816
Phe Phe His Lys Gln Asn Lys Met Leu Lys Lys Asp Lys Met Phe His           
            260                 265                 270                   

ttt tgg gta aat aca ttc ttc ata cca gga cca gag gaa acc tca gaa        864
Phe Trp Val Asn Thr Phe Phe Ile Pro Gly Pro Glu Glu Thr Ser Glu           
        275                 280                 285                       

aaa gta gaa aat gga agt cta tgt gat caa gaa atc gat agc att tgc        912
Lys Val Glu Asn Gly Ser Leu Cys Asp Gln Glu Ile Asp Ser Ile Cys           
    290                 295                 300                           

agt ata gag cgt gca gat aat gac aag gaa tat cta gta ctt act tta        960
Ser Ile Glu Arg Ala Asp Asn Asp Lys Glu Tyr Leu Val Leu Thr Leu           
305                 310                 315                 320           

aca aaa aat gat ctt gac aaa gca aat aaa gac aaa gcc aac cga tac       1008
Thr Lys Asn Asp Leu Asp Lys Ala Asn Lys Asp Lys Ala Asn Arg Tyr           
                325                 330                 335               

ttt tct cca aat ttt aag gtg aag ctg tac ttc aca aaa aca gta gag       1056
Phe Ser Pro Asn Phe Lys Val Lys Leu Tyr Phe Thr Lys Thr Val Glu           
            340                 345                 350                   

gag ccg tca aat cca gag gct agc agt tca act tct gta aca cca gat       1104
Glu Pro Ser Asn Pro Glu Ala Ser Ser Ser Thr Ser Val Thr Pro Asp           
        355                 360                 365                       

gtt agt gac aat gaa cct gat cat tat aga tat tct gac acc act gac       1152
Val Ser Asp Asn Glu Pro Asp His Tyr Arg Tyr Ser Asp Thr Thr Asp           
    370                 375                 380                           

tct gat cca gag aat gaa cct ttt gat gaa gat cag cat aca caa att       1200
Ser Asp Pro Glu Asn Glu Pro Phe Asp Glu Asp Gln His Thr Gln Ile           
385                 390                 395                 400           

aca aaa gtc tga                                                       1212
Thr Lys Val                                                               
                                                                          


<210>  4
<211>  403
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Thr Ala Ile Ile Lys Glu Ile Val Ser Arg Asn Lys Arg Arg Tyr 
1               5                   10                  15      


Gln Glu Asp Gly Phe Asp Leu Asp Leu Thr Tyr Ile Tyr Pro Asn Ile 
            20                  25                  30          


Ile Ala Met Gly Phe Pro Ala Glu Arg Leu Glu Gly Val Tyr Arg Asn 
        35                  40                  45              


Asn Ile Asp Asp Val Val Arg Phe Leu Asp Ser Lys His Lys Asn His 
    50                  55                  60                  


Tyr Lys Ile Tyr Asn Leu Cys Ala Glu Arg His Tyr Asp Thr Ala Lys 
65                  70                  75                  80  


Phe Asn Cys Arg Val Ala Gln Tyr Pro Phe Glu Asp His Asn Pro Pro 
                85                  90                  95      


Gln Leu Glu Leu Ile Lys Pro Phe Cys Glu Asp Leu Asp Gln Trp Leu 
            100                 105                 110         


Ser Glu Asp Asp Asn His Val Ala Ala Ile His Cys Lys Ala Gly Lys 
        115                 120                 125             


Gly Arg Thr Gly Val Met Ile Cys Ala Tyr Leu Leu His Arg Gly Lys 
    130                 135                 140                 


Phe Leu Lys Ala Gln Glu Ala Leu Asp Phe Tyr Gly Glu Val Arg Thr 
145                 150                 155                 160 


Arg Asp Lys Lys Gly Val Thr Ile Pro Ser Gln Arg Arg Tyr Val Tyr 
                165                 170                 175     


Tyr Tyr Ser Tyr Leu Leu Lys Asn His Leu Asp Tyr Arg Pro Val Ala 
            180                 185                 190         


Leu Leu Phe His Lys Met Met Phe Glu Thr Ile Pro Met Phe Ser Gly 
        195                 200                 205             


Gly Thr Cys Asn Pro Gln Phe Val Val Cys Gln Leu Lys Val Lys Ile 
    210                 215                 220                 


Tyr Ser Ser Asn Ser Gly Pro Thr Arg Arg Glu Asp Lys Phe Met Tyr 
225                 230                 235                 240 


Phe Glu Phe Pro Gln Pro Leu Pro Val Cys Gly Asp Ile Lys Val Glu 
                245                 250                 255     


Phe Phe His Lys Gln Asn Lys Met Leu Lys Lys Asp Lys Met Phe His 
            260                 265                 270         


Phe Trp Val Asn Thr Phe Phe Ile Pro Gly Pro Glu Glu Thr Ser Glu 
        275                 280                 285             


Lys Val Glu Asn Gly Ser Leu Cys Asp Gln Glu Ile Asp Ser Ile Cys 
    290                 295                 300                 


Ser Ile Glu Arg Ala Asp Asn Asp Lys Glu Tyr Leu Val Leu Thr Leu 
305                 310                 315                 320 


Thr Lys Asn Asp Leu Asp Lys Ala Asn Lys Asp Lys Ala Asn Arg Tyr 
                325                 330                 335     


Phe Ser Pro Asn Phe Lys Val Lys Leu Tyr Phe Thr Lys Thr Val Glu 
            340                 345                 350         


Glu Pro Ser Asn Pro Glu Ala Ser Ser Ser Thr Ser Val Thr Pro Asp 
        355                 360                 365             


Val Ser Asp Asn Glu Pro Asp His Tyr Arg Tyr Ser Asp Thr Thr Asp 
    370                 375                 380                 


Ser Asp Pro Glu Asn Glu Pro Phe Asp Glu Asp Gln His Thr Gln Ile 
385                 390                 395                 400 


Thr Lys Val 
            


<210>  5
<211>  1365
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe for centromere 10

<400>  5
cgacttttaa tatgaagata tttccatgtc taagattggc gtcaaatcgc ttgaaatctc       60

cacttgcaaa ttccacaaaa agtgtttttc aaaactgctc tgaataaagg aaggttccac      120

tctgtgagtt gaatacacac aacacaaagg atttactgag aattcttctg tctagcagta      180

aatgagaaat cccgcttcca acgaaggcct caaacgggtc taactaatca cttgcagact      240

ttacagacag agtctttcca aactgctcta tgaagagaaa ggtgaaactc tgtgaactga      300

acgcacagat gacaaagcag tttctgagaa tgcttctgtg tagtttttac acgaagatat      360

ttccatttca aagattagcc tcaaatcgct tgaaatctcc acttgcaaac tccacagaaa      420

gaatttttca aaactgctct gtctaaagga aggttcaact ctgtgacttg aatacacaca      480

acacaaagaa gtgactgaga attcttctgt ctagcattat atgaagaaat cccgtttcca      540

acgaaggcct caatgaagtc caaaaaagca cttgcaggct ttacaaacag agtgtttcca      600

aactgctcta tgaaaagaaa ggttaaactc tgtgagttga acgcacacat cacaaagtag      660

ttcttgagaa tgattctgtg tagtttttat acgaagatat ttcgttttct gccataggcc      720

tagaagcgct tgaaatctgc acttgcaaat tccaaaaaca gagtgtttca aatctgctct      780

ctctaaagga aggttcaaat ctgtgagttg aatacaaaca acacaaagac gttactgaga      840

attcttctgt ctagcattat atgaggaaat cccgtttcca acgaagggct caaagagggc      900

caattatcca cctgcagact tacaaagagt gtatttccaa actgctcgat taaagaaagg      960

ttaaactctg tgagttgaac acacacatca caaagtgttt tctgagaatg attttgtcta     1020

gttttaatac gaagatatat cctttcctat cactgtcttc gaagcgtttg aaatctgcac     1080

ttgcaaattc caaaaacaga gtgtttcaac tctgctctct ctcaagaaag gttcaactct     1140

gtgagttgaa tacacacaac acaaagaagt tactgagaat tcttctgtct agtgttgtat     1200

gaagaaatcc cgtttccaac gaaggcctca aagaggtcca aatatccact tgcagacttt     1260

agaaatagag tgtttccaaa ctgctctatg aaaagaaagg ttaaactctg tgagttgaag     1320

gcacacatca caaactagtt tctacgaatg actctgtgtc gagct                     1365


