                         SEQUENCE LISTING

<110>  E. I. du Pont de Nemours and Company
       
       
       
       
 
<120>  GENE INACTIVATION ALLOWING IMMEDIATE GROWTH ON XYLOSE MEDIUM BY 
       ENGINEERED ZYMOMONAS

<130>  CL5570PCT

<160>  72    

<170>  PatentIn version 3.5

<210>  1
<211>  1023
<212>  DNA
<213>  Zymomonas mobilis

<400>  1
atgaacactt ctacgcaaaa acccgctcat ttcgacaaga tttcgatcaa agggattgat       60

aaatccgcaa cccgtgtagc gttaggcaca tgggctattg gtggctggat gtggggcggc      120

actgatgacg atgcctccat taaaaccatt catcgggcga ttgatcttgg tatcaatatc      180

atcgacaccg ctccggctta tggccgtggc catgctgaag aagtcgttgg taaagccatc      240

aaaggtcaac gcgataattt gattattgcg accaaagtcg gccttgattg gactttaacc      300

cccgaccaat cgatgcgccg taacagttca gccagccgta tcaaaaaaga aatcgaagat      360

tctctgcgcc gccttggcac tgattatatc gacctttatc aggtgcattg gccggatccg      420

ctggttccga ttgaagaaac cgcaacaata ttggaagccc tcagaaaaga aggcaaaatc      480

cgttctatcg gcgtttccaa ttattccgtt cagcagatgg acgagttcaa gaaatatgcc      540

gagctggccg tttcgcagtc gccttataat ctgtttgaac gcgaaataga caaagacatc      600

ctgccctatg ccaagaaaaa cgatctggtc gttttaggct atggtgcgct ttgccgtggt      660

ttactttctg gcagaatgac ggcggatcgt gcctttacag gcgatgattt acggaaaaca      720

gacccgaaat tccagaaacc gcgctttgaa cattatctgg ccgcggttga agaactgaag      780

aaactcgcca aagagcatta caataaatcg gtgttggctt tggctatccg ctggatgttg      840

gagcaagggc ccactttagc actttggggc gctcgcaagc cggaacagat cgacggtatt      900

gatgaagttt ttggctggca gatatcggat gaagatctga aacagattga tgctattctg      960

gccaagaata tccccaatcc tatcggtgca gaatttatgg cacccccgcc acgcgataaa     1020

taa                                                                   1023


<210>  2
<211>  340
<212>  PRT
<213>  Zymomonas mobilis

<400>  2

Met Asn Thr Ser Thr Gln Lys Pro Ala His Phe Asp Lys Ile Ser Ile 
1               5                   10                  15      


Lys Gly Ile Asp Lys Ser Ala Thr Arg Val Ala Leu Gly Thr Trp Ala 
            20                  25                  30          


Ile Gly Gly Trp Met Trp Gly Gly Thr Asp Asp Asp Ala Ser Ile Lys 
        35                  40                  45              


Thr Ile His Arg Ala Ile Asp Leu Gly Ile Asn Ile Ile Asp Thr Ala 
    50                  55                  60                  


Pro Ala Tyr Gly Arg Gly His Ala Glu Glu Val Val Gly Lys Ala Ile 
65                  70                  75                  80  


Lys Gly Gln Arg Asp Asn Leu Ile Ile Ala Thr Lys Val Gly Leu Asp 
                85                  90                  95      


Trp Thr Leu Thr Pro Asp Gln Ser Met Arg Arg Asn Ser Ser Ala Ser 
            100                 105                 110         


Arg Ile Lys Lys Glu Ile Glu Asp Ser Leu Arg Arg Leu Gly Thr Asp 
        115                 120                 125             


Tyr Ile Asp Leu Tyr Gln Val His Trp Pro Asp Pro Leu Val Pro Ile 
    130                 135                 140                 


Glu Glu Thr Ala Thr Ile Leu Glu Ala Leu Arg Lys Glu Gly Lys Ile 
145                 150                 155                 160 


Arg Ser Ile Gly Val Ser Asn Tyr Ser Val Gln Gln Met Asp Glu Phe 
                165                 170                 175     


Lys Lys Tyr Ala Glu Leu Ala Val Ser Gln Ser Pro Tyr Asn Leu Phe 
            180                 185                 190         


Glu Arg Glu Ile Asp Lys Asp Ile Leu Pro Tyr Ala Lys Lys Asn Asp 
        195                 200                 205             


Leu Val Val Leu Gly Tyr Gly Ala Leu Cys Arg Gly Leu Leu Ser Gly 
    210                 215                 220                 


Arg Met Thr Ala Asp Arg Ala Phe Thr Gly Asp Asp Leu Arg Lys Thr 
225                 230                 235                 240 


Asp Pro Lys Phe Gln Lys Pro Arg Phe Glu His Tyr Leu Ala Ala Val 
                245                 250                 255     


Glu Glu Leu Lys Lys Leu Ala Lys Glu His Tyr Asn Lys Ser Val Leu 
            260                 265                 270         


Ala Leu Ala Ile Arg Trp Met Leu Glu Gln Gly Pro Thr Leu Ala Leu 
        275                 280                 285             


Trp Gly Ala Arg Lys Pro Glu Gln Ile Asp Gly Ile Asp Glu Val Phe 
    290                 295                 300                 


Gly Trp Gln Ile Ser Asp Glu Asp Leu Lys Gln Ile Asp Ala Ile Leu 
305                 310                 315                 320 


Ala Lys Asn Ile Pro Asn Pro Ile Gly Ala Glu Phe Met Ala Pro Pro 
                325                 330                 335     


Pro Arg Asp Lys 
            340 


<210>  3
<211>  305
<212>  DNA
<213>  Zymomonas mobilis

<400>  3
gttcgatcaa caacccgaat cctatcgtaa tgatgttttg cccgatcagc ctcaatcgac       60

aattttacgc gtttcgatcg aagcagggac gacaattggc tgggaacggt atactggaat      120

aaatggtctt cgttatggta ttgatgtttt tggtgcatcg gccccggcga atgatctata      180

tgctcatttc ggcttgaccg cagtcggcat cacgaacaag gtgttggccg cgatcgccgg      240

taagtcggca cgttaaaaaa tagctatgga atataatagc tacttaataa gttaggagaa      300

taaac                                                                  305


<210>  4
<211>  304
<212>  DNA
<213>  artificial sequence

<220>
<223>  mutated promoter

<400>  4
gttcgatcaa caacccgaat cctatcgtaa tgatgttttg cccgatcagc ctcaatcgac       60

aattttacgc gtttcgatcg aagcagggac gacaattggc tgggaacggt atacttgaat      120

aaatggtctt cgttatggta ttgatgtttt tggtgcatcg gccccggcga atgatctata      180

tgctcatttc ggcttgaccg cagtcggcat cacgaacaag gtgttggccg cgatcgccgg      240

taagtcggca cgttaaaaaa tagctatgga atataatagc tactaataag ttaggagaat      300

aaac                                                                   304


<210>  5
<211>  304
<212>  DNA
<213>  artificial sequence

<220>
<223>  mutant promoter

<400>  5
gttcgatcaa caacccgaat cctatcgtaa tgatgttttg cccgatcagc ctcaatcgac       60

aattttacgc gtttcgatcg aagcagggac gacaattggc tgggaacggt atactggaat      120

aaatggtctt cgttatggta ttgatgtttt tggtgcatcg gccccggcga atgatctata      180

tgctcatttc ggcttgaccg cagtcggcat cacgaataag gtgttggccg cgatcgccgg      240

taagtcggca cgttaaaaaa tagctatgga atataatagc tactaataag ttaggagaat      300

aaac                                                                   304


<210>  6
<211>  304
<212>  DNA
<213>  artificial sequence

<220>
<223>  double mutation promoter

<400>  6
gttcgatcaa caacccgaat cctatcgtaa tgatgttttg cccgatcagc ctcaatcgac       60

aattttacgc gtttcgatcg aagcagggac gacaattggc tgggaacggt atacttgaat      120

aaatggtctt cgttatggta ttgatgtttt tggtgcatcg gccccggcga atgatctata      180

tgctcatttc ggcttgaccg cagtcggcat cacgaataag gtgttggccg cgatcgccgg      240

taagtcggca cgttaaaaaa tagctatgga atataatagc tactaataag ttaggagaat      300

aaac                                                                   304


<210>  7
<211>  305
<212>  DNA
<213>  artificial sequence

<220>
<223>  promoter with mutation

<400>  7
gttcgatcaa caacccgaat cctatcgtaa tgatgttttg cccgatcagc ctcaatcgac       60

aattttacgc gtttcgatcg aagcagggac gacaattggc tgggaacggt atacttgaat      120

aaatggtctt cgttatggta ttgatgtttt tggtgcatcg gccccggcga atgatctata      180

tgctcatttc ggcttgaccg cagtcggcat cacgaacaag gtgttggccg cgatcgccgg      240

taagtcggca cgttaaaaaa tagctatgga atataatagc tacttaataa gttaggagaa      300

taaac                                                                  305


<210>  8
<211>  305
<212>  DNA
<213>  artificial sequence

<220>
<223>  promoter with mutation

<400>  8
gttcgatcaa caacccgaat cctatcgtaa tgatgttttg cccgatcagc ctcaatcgac       60

aattttacgc gtttcgatcg aagcagggac gacaattggc tgggaacggt atactggaat      120

aaatggtctt cgttatggta ttgatgtttt tggtgcatcg gccccggcga atgatctata      180

tgctcatttc ggcttgaccg cagtcggcat cacgaataag gtgttggccg cgatcgccgg      240

taagtcggca cgttaaaaaa tagctatgga atataatagc tacttaataa gttaggagaa      300

taaac                                                                  305


<210>  9
<211>  305
<212>  DNA
<213>  artificial sequence

<220>
<223>  promoter with mutations

<400>  9
gttcgatcaa caacccgaat cctatcgtaa tgatgttttg cccgatcagc ctcaatcgac       60

aattttacgc gtttcgatcg aagcagggac gacaattggc tgggaacggt atacttgaat      120

aaatggtctt cgttatggta ttgatgtttt tggtgcatcg gccccggcga atgatctata      180

tgctcatttc ggcttgaccg cagtcggcat cacgaataag gtgttggccg cgatcgccgg      240

taagtcggca cgttaaaaaa tagctatgga atataatagc tacttaataa gttaggagaa      300

taaac                                                                  305


<210>  10
<211>  305
<212>  DNA
<213>  artificial sequence

<220>
<223>  promoter with mutation

<400>  10
gttcgatcaa caacccgaat cctatcgtaa tgatgttttg cccgatcagc ctcaatcgac       60

aattttacgc gtttcgatcg aagcagggac gacaattggc tgggaacggt atacttgaat      120

aaatggtctt cgttatggta ttgatgtttt tggtgcatcg gccccggcga atgatctata      180

tgctcatttc ggcttgaccg cagtcggcat cacgaacaag gtgttggccg cgatcgccgg      240

taagtcggca cgttaaaaaa tagctatgga atatagtagc tacttaataa gttaggagaa      300

taaac                                                                  305


<210>  11
<211>  305
<212>  DNA
<213>  artificial sequence

<220>
<223>  promoter with mutation

<400>  11
gttcgatcaa caacccgaat cctatcgtaa tgatgttttg cccgatcagc ctcaatcgac       60

aattttacgc gtttcgatcg aagcagggac gacaattggc tgggaacggt atactggaat      120

aaatggtctt cgttatggta ttgatgtttt tggtgcatcg gccccggcga atgatctata      180

tgctcatttc ggcttgaccg cagtcggcat cacgaataag gtgttggccg cgatcgccgg      240

taagtcggca cgttaaaaaa tagctatgga atatagtagc tacttaataa gttaggagaa      300

taaac                                                                  305


<210>  12
<211>  305
<212>  DNA
<213>  artificial sequence

<220>
<223>  promoter with mutations

<400>  12
gttcgatcaa caacccgaat cctatcgtaa tgatgttttg cccgatcagc ctcaatcgac       60

aattttacgc gtttcgatcg aagcagggac gacaattggc tgggaacggt atacttgaat      120

aaatggtctt cgttatggta ttgatgtttt tggtgcatcg gccccggcga atgatctata      180

tgctcatttc ggcttgaccg cagtcggcat cacgaataag gtgttggccg cgatcgccgg      240

taagtcggca cgttaaaaaa tagctatgga atatagtagc tacttaataa gttaggagaa      300

taaac                                                                  305


<210>  13
<211>  1182
<212>  DNA
<213>  artificial sequence

<220>
<223>  codon optimized coding region of Actinoplanes missouriensis 
       xylose isomerase for expression in Zymomonas

<400>  13
atgagtgtcc aagctacacg cgaagataaa tttagctttg gcttgtggac agttgggtgg       60

caggcccgtg acgcctttgg tgatgccacg cgtaccgccc ttgatccggt cgaagcagtt      120

cataaacttg ccgaaatcgg agcctatggc attacatttc atgatgatga cttggtcccc      180

ttcggttcgg atgctcagac acgggatggt atcattgctg gtttcaaaaa agcccttgat      240

gaaaccggtt tgatcgttcc tatggttacc acaaatttgt ttacgcatcc ggtttttaaa      300

gacggcgggt ttacctctaa cgatcgttct gtgcgccggt atgctatccg gaaagttttg      360

agacagatgg atcttggtgc ggaactgggg gccaaaacgc ttgtcttatg gggcggcaga      420

gaaggtgcgg aatatgattc agcaaaagat gttagtgccg ccttggatcg ttatcgcgaa      480

gcactgaatc ttctggcaca atatagcgaa gacagaggct atggacttcg ttttgcaatt      540

gaaccgaaac ctaatgaacc acgtggcgat attctgctgc ctaccgcagg ccatgctatt      600

gcctttgtgc aagaattaga acggcccgaa ctttttggca tcaatccaga aaccgggcat      660

gaacagatgt caaacttgaa ttttacccag gggattgctc aggctttgtg gcataaaaaa      720

ctttttcata ttgatttgaa tggacaacat ggtcccaagt ttgatcagga ccttgtcttt      780

ggtcatggtg accttttaaa tgcctttagc ctggtcgatt tgttagaaaa tggtccagat      840

ggtgccccgg cttatgatgg cccgcgccat tttgattata aaccatctcg tactgaagat      900

tatgatggcg tttgggaatc agcgaaagcc aatatccgta tgtatctttt attaaaagaa      960

cgtgccaaag cgtttagagc tgatccggaa gttcaggaag cactggcagc aagcaaagtt     1020

gccgaattga aaaccccaac gttgaatcct ggtgaaggct atgcagaact gttagcagat     1080

cgcagtgctt ttgaagatta tgatgccgat gctgttggtg cgaaaggttt tggtttcgtg     1140

aaattgaacc aattggccat tgaacattta ttaggtgccc gc                        1182


<210>  14
<211>  945
<212>  DNA
<213>  Zymomonas mobilis

<400>  14
atgaacactt ctacgcaaaa acccgctcat ttcgacaaga tttcgatcaa agggattgat       60

aaatccgcaa cccgtgtagc gttaggcaca tgggctattg gtggctggat gtggggcggc      120

actgatgacg atgcctccat taaaaccatt catcgggcga ttgatcttgg tatcaatatc      180

atcgacaccg ctccggctta tggccgtggc catgctgaag aagtcgttgg taaagccatc      240

aaaggtcaac gcgataattt gattattgcg accaaagtcg gccttgattg gactttaacc      300

cccgaccaat cgatgcgccg taacagttca gccagccgta tcaaaaaaga aatcgaagat      360

tctctgcgcc gccttggcac tgattatatc gacctttatc aggtgcattg gccggatccg      420

ctggttccga ttgaagaaac cgcaacaata ttggaagccc tcagaaaaga aggcaaaatc      480

cgttctatcg gcgtttccaa ttattccgtt cagcagatgg acgagttcaa gaaatatgcc      540

gagctgctgg tcgttttagg ctatggtgcg ctttgccgtg gtttactttc tggcagaatg      600

acggcggatc gtgcctttac aggcgatgat ttacggaaaa cagacccgaa attccagaaa      660

ccgcgctttg aacattatct ggccgcggtt gaagaactga agaaactcgc caaagagcat      720

tacaataaat cggtgttggc tttggctatc cgctggatgt tggagcaagg gcccacttta      780

gcactttggg gcgctcgcaa gccggaacag atcgacggta ttgatgaagt ttttggctgg      840

cagatatcgg atgaagatct gaaacagatt gatgctattc tggccaagaa tatccccaat      900

cctatcggtg cagaatttat ggcacccccg ccacgcgata aataa                      945


<210>  15
<211>  10250
<212>  DNA
<213>  artificial sequence

<220>
<223>  constructed plasmid

<400>  15
ctagtgttcg atcaacaacc cgaatcctat cgtaatgatg ttttgcccga tcagcctcaa       60

tcgacaattt tacgcgtttc gatcgaagca gggacgacaa ttggctggga acggtatact      120

tgaataaatg gtcttcgtta tggtattgat gtttttggtg catcggcccc ggcgaatgat      180

ctatatgctc atttcggctt gaccgcagtc ggcatcacga acaaggtgtt ggccgcgatc      240

gccggtaagt cggcacgtta aaaaatagct atggaatata atagctacta ataagttagg      300

agaataaaca tgagtgtcca agctacacgc gaagataaat ttagctttgg cttgtggaca      360

gttgggtggc aggcccgtga cgcctttggt gatgccacgc gtaccgccct tgatccggtc      420

gaagcagttc ataaacttgc cgaaatcgga gcctatggca ttacatttca tgatgatgac      480

ttggtcccct tcggttcgga tgctcagaca cgggatggta tcattgctgg tttcaaaaaa      540

gcccttgatg aaaccggttt gatcgttcct atggttacca caaatttgtt tacgcatccg      600

gtttttaaag acggcgggtt tacctctaac gatcgttctg tgcgccggta tgctatccgg      660

aaagttttga gacagatgga tcttggtgcg gaactggggg ccaaaacgct tgtcttatgg      720

ggcggcagag aaggtgcgga atatgattca gcaaaagatg ttagtgccgc cttggatcgt      780

tatcgcgaag cactgaatct tctggcacaa tatagcgaag acagaggcta tggacttcgt      840

tttgcaattg aaccgaaacc taatgaacca cgtggcgata ttctgctgcc taccgcaggc      900

catgctattg cctttgtgca agaattagaa cggcccgaac tttttggcat caatccagaa      960

accgggcatg aacagatgtc aaacttgaat tttacccagg ggattgctca ggctttgtgg     1020

cataaaaaac tttttcatat tgatttgaat ggacaacatg gtcccaagtt tgatcaggac     1080

cttgtctttg gtcatggtga ccttttaaat gcctttagcc tggtcgattt gttagaaaat     1140

ggtccagatg gtgccccggc ttatgatggc ccgcgccatt ttgattataa accatctcgt     1200

actgaagatt atgatggcgt ttgggaatca gcgaaagcca atatccgtat gtatctttta     1260

ttaaaagaac gtgccaaagc gtttagagct gatccggaag ttcaggaagc actggcagca     1320

agcaaagttg ccgaattgaa aaccccaacg ttgaatcctg gtgaaggcta tgcagaactg     1380

ttagcagatc gcagtgcttt tgaagattat gatgccgatg ctgttggtgc gaaaggtttt     1440

ggtttcgtga aattgaacca attggccatt gaacatttat taggtgcccg ctagtctaga     1500

tgactgtata aaaccacagc caatcaaacg aaaccaggct atactcaagc ctggtttttt     1560

gatggatttt cagcgtggcg caggcaggtt ttatcttaac ccgacactgg cgggacaccc     1620

cgcaagggac agaagtctcc ttctggctgg cgacggacaa cgggccaagc ttccagttac     1680

tcaatacgta acaataatca gtttatccta actatagaat cgcatgagaa gcgataacgt     1740

ttcaccataa gcaatatatt cattgcaaca gtggaattgc cttatgcgtc aaggaaggat     1800

agatcattga cggactgagt tcaaaaagag actggtctaa aagattttaa gaaaggtttc     1860

gatatgtata tcgggataga tcttggcacc tcgggcgtaa aagttatttt gctcaacgag     1920

cagggtgagg tggttgctgc gcaaacggaa aagctgaccg tttcgcgccc gcatccactc     1980

tggtcggaac aagacccgga acagtggtgg caggcaactg atcgcgcaat gaaagctctg     2040

ggcgatcagc attctctgca ggacgttaaa gcattgggta ttgccggcca gatgcacgga     2100

gcaaccttgc tggatgctca gcaacgggtg ttacgccctg ccattttgtg gaacgacggg     2160

cgctgtgcgc aagagtgcac tttgctggaa gcgcgagttc cgcaatcgcg ggtgattacc     2220

ggcaacctga tgatgcccgg atttactgcg cctaaattgc tatgggttca gcggcatgag     2280

ccggagatat tccgtcaaat cgacaaagta ttattaccga aagattactt gcgtctgcgt     2340

atgacggggg agtttgccag cgatatgtct gacgcagctg gcaccatgtg gctggatgtc     2400

gcaaagcgtg actggagtga cgtcatgctg caggcttgcg acttatctcg tgaccagatg     2460

cccgcattat acgaaggcag cgaaattact ggtgctttgt tacctgaagt tgcgaaagcg     2520

tggggtatgg cgacggtgcc agttgtcgca ggcggtggcg acaatgcagc tggtgcagtt     2580

ggtgtgggaa tggttgatgc taatcaggca atgttatcgc tggggacgtc gggggtctat     2640

tttgctgtca gcgaagggtt cttaagcaag ccagaaagcg ccgtacatag cttttgccat     2700

gcgctaccgc aacgttggca tttaatgtct gtgatgctga gtgcagcgtc gtgtctggat     2760

tgggccgcga aattaaccgg cctgagcaat gtcccagctt taatcgctgc agctcaacag     2820

gctgatgaaa gtgccgagcc agtttggttt ctgccttatc tttccggcga gcgtacgcca     2880

cacaataatc cccaggcgaa gggggttttc tttggtttga ctcatcaaca tggccccaat     2940

gaactggcgc gagcagtgct ggaaggcgtg ggttatgcgc tggcagatgg catggatgtc     3000

gtgcatgcct gcggtattaa accgcaaagt gttacgttga ttgggggcgg ggcgcgtagt     3060

gagtactggc gtcagatgct ggcggatatc agcggtcagc agctcgatta ccgtacgggg     3120

ggggatgtgg ggccagcact gggcgcagca aggctggcgc agatcgcggc gaatccagag     3180

aaatcgctca ttgaattgtt gccgcaacta ccgttagaac agtcgcatct accagatgcg     3240

cagcgttatg ccgcttatca gccacgacga gaaacgttcc gtcgcctcta tcagcaactt     3300

ctgccattaa tggcgtaaac gttatcccct gcctgaccgg gtgggggata attcacatct     3360

atatatctca gtaattaatt aatatttagt acgaatttat tctgaaaatc atttgttaat     3420

ggcatttttc agttttgtct ttcgttggtt actcgtaatg tatcgctggt agatatggag     3480

atcgttatga aaacctcaaa gactgtggca aaactattat ttgttgtcgg ggcgctggtt     3540

tatctggttg ggctatggat ctcatgccca ttgttaagtg gaaaaggcta ttttcttggc     3600

gtgttaatga cagcaacttt tggcaactat gcgaattcgc gatcgcataa cttcgtataa     3660

tgtatgctat acgaagttat gcggccgcag cacaggatga cgcctaacaa ttcattcaag     3720

ccgacaccgc ttcgcggcgc ggcttaattc aggagttaaa catcatgagg gaagcggtga     3780

tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac     3840

cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca     3900

gtgatattga tttgctggtt acggtgactg taaggcttga tgaaacaacg cggcgagctt     3960

tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg     4020

tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg     4080

aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca     4140

cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg     4200

taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc     4260

taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg     4320

tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg     4380

atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg     4440

aagctaggca ggcttatctt ggacaagaag atcgcttggc ctcgcgcgca gatcagttgg     4500

aagaatttgt tcactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa     4560

caattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agagagctgg     4620

ggaagactat gcgcgatctg ttgaaggtgg ttctaagcct cgtacttgcg atggcatcgg     4680

ggcaggcact tgctgacctg ccttaattaa ataacttcgt ataatgtatg ctatacgaag     4740

ttatggccgg cccgttactt gctgggtgaa gaaccgatcg aagtccgtgc ttacacctac     4800

agcgatccga atgatgaacg tttcgttgaa gtcgaagatc gtattatttg gcagatgcgc     4860

ttcagaagcg gtgctctgtc tcatggtgca tcttcttatt cgaccacgac gacttcacgt     4920

ttctcggtgc agggcgacaa agctgttctg ttgatggatc cggctaccgg atattatcag     4980

aatttgattt ctgtccagac cccaggccat gctaaccagt cgatgatgcc acagttcatc     5040

atgccagcga acaaccagtt ctctgcacag ttggatcatc tggctgaagc cgtcatcaat     5100

aacaaaccag ttcgtagccc gggtgaagaa ggtatgcagg atgtgcgcct gattcaggcc     5160

atttatgaag cagctcgtac cggtcgcccc gtcaacacgg attggggtta tgtccgtcag     5220

ggtggttatt gattctgact taacctattt gggttaaaca gacttatttt tcctgtttta     5280

ggaaaatagt taaaaaggcg tcattggttc ttccaatgac gccttttttt ataaacaaaa     5340

aaatcctttt gtcggtttta taaaaatact tcatattttg ataagccgtc ttaaaaatat     5400

aataaatttt tataatattt atccgatcaa aggacccctt tatgctagaa gtcattatat     5460

cggcattact accgattata attactttaa tgataggttt tttcgctggc tggcgtggtg     5520

aatttacggc aaatcaagcc tcgaccttga ataaaatggt cttacgctat gccttaccta     5580

tgactttatt ctctgggatt ttatcacttc ccaaaacaca gattttatcg tcgggttctg     5640

ccgcaattat tttactttta gccatggctg gcggctatct aattacactt gggataggat     5700

attttgtctg ccagcgccca gtgaatgaat ctgctctttt agctctttct gttagcgcac     5760

ctgcagttcc ttttgttggc ataacagttc tagggcattt atttggcact gccagcacga     5820

tattggtttc aatatgtagc ctgatgatga acctcgtcca ggttcccgtt acctttttct     5880

tttgtcagcg tattctccaa aaaagaatac tgacaaaata gccacggata gttctttttt     5940

ttctcatatc agacatgctt ttaccgaacc tattgttatt gcccctattc tggctcttat     6000

ctgtgtcagt ctctctattc ctttccctga aaccttaaaa tcttctttaa tgctactagg     6060

aaaagcgacc ggaggcgttg cgcttttctc ttctggtata attttatttt ctcgaaaagt     6120

tattttaagt aaaacagtag catctttagt tttatcaaaa aatattatta ttccaacagc     6180

ggtattggtt cttgcgtcgg tacccaattc gccctatagt gagtcgtatt acgcgcgctc     6240

actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg     6300

ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg     6360

cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt     6420

aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc     6480

gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca     6540

agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc     6600

caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt     6660

tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac     6720

aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc     6780

ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt     6840

aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta     6900

tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt     6960

caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc     7020

ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa     7080

gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt     7140

aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt     7200

ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc     7260

atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg     7320

gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg     7380

gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac     7440

atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca     7500

aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta     7560

actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat     7620

aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa     7680

tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag     7740

ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat     7800

agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt     7860

tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg     7920

aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga     7980

gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta     8040

atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa     8100

gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact     8160

gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca     8220

tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt     8280

accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg     8340

ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag     8400

cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta     8460

agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat     8520

ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg     8580

tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc     8640

ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac     8700

cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc     8760

gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt     8820

tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag     8880

cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg     8940

cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc     9000

tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag     9060

ctcgtccaga aaagacagca ttccttctca ataaagaaat attatttttt gtttttgaaa     9120

aatttttcca aaatctagaa tgctacatta aatatacaaa aatattatta tacaaataag     9180

gcttttaaat acccatattt tttagaattt ctttacaaag aaacatgtta aatatagatt     9240

tagagattaa tatcagccat ttttatcaaa aattcttttt ttgttttata atattatgct     9300

gcaaaactaa taaaaacgcc ctttcgaaat taacgatcac ccacaagaaa taattatctg     9360

acagcgctta ccaatcaatt attgccgaac gcagagtccc gtattaggac ggtcaacaat     9420

ctaaaccgtt tttcagaaaa tattgcttta taagcctcaa aacttaaaag ctgcggtatt     9480

ttaatatacc aaaattttct ggaaaagccg gcgaatcaga taacagttcc gcacaggtga     9540

gaaccacgac ggatcttctc tgaattgttg gttagttaag aaagaaacaa ggattatgac     9600

gaacaaaatc tcgtcttcag ataatctttc caatgctgtt tcagcaacgg atgacaacgc     9660

ttcccgtacg ccaaatctga cccgtcgcgc tctcgttggt ggtggtgttg gactggccgc     9720

agctggcgcc ttagccagtg gtcttcaggc agcgacgctt cctgctggtg ccagccaggt     9780

tccgaccacg cctgcaggtc gcccgatgcc ttacgcgatc cgcccgatgc cggaagatcg     9840

tcgtttcggt tatgctatcg tcggtctggg taaatatgcc cttaaccaga ttttaccggg     9900

ttttgccgga tgccagcatt cccgcatcga agctttggtc agcggtaacg ctgaaaaagc     9960

taaaatcgtt gccgctgaat atggcgtcga tccccgtaaa atttatgatt acagcaactt    10020

cgacaagatc gctaaagatc caaaaatcga cgctgtttac atcattttgc caaactcttt    10080

gcatgctgaa tttgctatcc gtgctttcaa agccggcaag catgttatgt gtgaaaagcc    10140

gatggcaacc tctgttgctg attgtcagcg gatgatcgat gcagccaagg ctgctaataa    10200

aaagctgatg atcggttacc gttgccacta tgatccaatg aaccgtgcaa               10250


<210>  16
<211>  1186
<212>  DNA
<213>  artificial sequence

<220>
<223>  sonstructed fragment

<400>  16
gtccagaaaa gacagcattc cttctcaata aagaaatatt attttttgtt tttgaaaaat       60

ttttccaaaa tctagaatgc tacattaaat atacaaaaat attattatac aaataaggct      120

tttaaatacc catatttttt agaatttctt tacaaagaaa catgttaaat atagatttag      180

agattaatat cagccatttt tatcaaaaat tctttttttg ttttataata ttatgctgca      240

aaactaataa aaacgccctt tcgaaattaa cgatcaccca caagaaataa ttatctgaca      300

gcgcttacca atcaattatt gccgaacgca gagtcccgta ttaggacggt caacaatcta      360

aaccgttttt cagaaaatat tgctttataa gcctcaaaac ttaaaagctg cggtatttta      420

atataccaaa attttctgga aaagccggcg aatcagataa cagttccgca caggtgagaa      480

ccacgacgga tcttctctga attgttggtt agttaagaaa gaaacaagga ttatgacgaa      540

caaaatctcg tcttcagata atctttccaa tgctgtttca gcaacggatg acaacgcttc      600

ccgtacgcca aatctgaccc gtcgcgctct cgttggtggt ggtgttggac tggccgcagc      660

tggcgcctta gccagtggtc ttcaggcagc gacgcttcct gctggtgcca gccaggttcc      720

gaccacgcct gcaggtcgcc cgatgcctta cgcgatccgc ccgatgccgg aagatcgtcg      780

tttcggttat gctatcgtcg gtctgggtaa atatgccctt aaccagattt taccgggttt      840

tgccggatgc cagcattccc gcatcgaagc tttggtcagc ggtaacgctg aaaaagctaa      900

aatcgttgcc gctgaatatg gcgtcgatcc ccgtaaaatt tatgattaca gcaacttcga      960

caagatcgct aaagatccaa aaatcgacgc tgtttacatc attttgccaa actctttgca     1020

tgctgaattt gctatccgtg ctttcaaagc cggcaagcat gttatgtgtg aaaagccgat     1080

ggcaacctct gttgctgatt gtcagcggat gatcgatgca gccaaggctg ctaataaaaa     1140

gctgatgatc ggttaccgtt gccactatga tccaatgaac cgtgca                    1186


<210>  17
<211>  1302
<212>  DNA
<213>  Zymomonas mobilis

<400>  17
atgacgaaca aaatctcgtc ttcagataat ctttccaatg ctgtttcagc aacggatgac       60

aacgcttccc gtacgccaaa tctgacccgt cgcgctctcg ttggtggtgg tgttggactg      120

gccgcagctg gcgccttagc cagtggtctt caggcagcga cgcttcctgc tggtgccagc      180

caggttccga ccacgcctgc aggtcgcccg atgccttacg cgatccgccc gatgccggaa      240

gatcgtcgtt tcggttatgc tatcgtcggt ctgggtaaat atgcccttaa ccagatttta      300

ccgggttttg ccggatgcca gcattcccgc atcgaagctt tggtcagcgg taacgctgaa      360

aaagctaaaa tcgttgccgc tgaatatggc gtcgatcccc gtaaaattta tgattacagc      420

aacttcgaca agatcgctaa agatccaaaa atcgacgctg tttacatcat tttgccaaac      480

tctttgcatg ctgaatttgc tatccgtgct ttcaaagccg gcaagcatgt tatgtgtgaa      540

aagccgatgg caacctctgt tgctgattgt cagcggatga tcgatgcagc caaggctgct      600

aataaaaagc tgatgatcgg ttaccgttgc cactatgatc caatgaaccg tgcagcggta      660

aaattgatcc gtgaaaacca gttgggtaaa ctgggcatgg ttaccaccga caactcagac      720

gttatggatc agaacgatcc tgcacagcag tggcgtctgc gtcgtgaact cgccggtggc      780

ggttctttga tggatatcgg tatttatggc ttgaacggta cccgttactt gctgggtgaa      840

gaaccgatcg aagtccgtgc ttacacctac agcgatccga atgatgaacg tttcgttgaa      900

gtcgaagatc gtattatttg gcagatgcgc ttcagaagcg gtgctctgtc tcatggtgca      960

tcttcttatt cgaccacgac gacttcacgt ttctcggtgc agggcgacaa agctgttctg     1020

ttgatggatc cggctaccgg atattatcag aatttgattt ctgtccagac cccaggccat     1080

gctaaccagt cgatgatgcc acagttcatc atgccagcga acaaccagtt ctctgcacag     1140

ttggatcatc tggctgaagc cgtcatcaat aacaaaccag ttcgtagccc gggtgaagaa     1200

ggtatgcagg atgtgcgcct gattcaggcc atttatgaag cagctcgtac cggtcgcccc     1260

gtcaacacgg attggggtta tgtccgtcag ggtggttatt ga                        1302


<210>  18
<211>  1446
<212>  DNA
<213>  artificial sequence

<220>
<223>  constructed fragment

<400>  18
cgttacttgc tgggtgaaga accgatcgaa gtccgtgctt acacctacag cgatccgaat       60

gatgaacgtt tcgttgaagt cgaagatcgt attatttggc agatgcgctt cagaagcggt      120

gctctgtctc atggtgcatc ttcttattcg accacgacga cttcacgttt ctcggtgcag      180

ggcgacaaag ctgttctgtt gatggatccg gctaccggat attatcagaa tttgatttct      240

gtccagaccc caggccatgc taaccagtcg atgatgccac agttcatcat gccagcgaac      300

aaccagttct ctgcacagtt ggatcatctg gctgaagccg tcatcaataa caaaccagtt      360

cgtagcccgg gtgaagaagg tatgcaggat gtgcgcctga ttcaggccat ttatgaagca      420

gctcgtaccg gtcgccccgt caacacggat tggggttatg tccgtcaggg tggttattga      480

ttctgactta acctatttgg gttaaacaga cttatttttc ctgttttagg aaaatagtta      540

aaaaggcgtc attggttctt ccaatgacgc ctttttttat aaacaaaaaa atccttttgt      600

cggttttata aaaatacttc atattttgat aagccgtctt aaaaatataa taaattttta      660

taatatttat ccgatcaaag gaccccttta tgctagaagt cattatatcg gcattactac      720

cgattataat tactttaatg ataggttttt tcgctggctg gcgtggtgaa tttacggcaa      780

atcaagcctc gaccttgaat aaaatggtct tacgctatgc cttacctatg actttattct      840

ctgggatttt atcacttccc aaaacacaga ttttatcgtc gggttctgcc gcaattattt      900

tacttttagc catggctggc ggctatctaa ttacacttgg gataggatat tttgtctgcc      960

agcgcccagt gaatgaatct gctcttttag ctctttctgt tagcgcacct gcagttcctt     1020

ttgttggcat aacagttcta gggcatttat ttggcactgc cagcacgata ttggtttcaa     1080

tatgtagcct gatgatgaac ctcgtccagg ttcccgttac ctttttcttt tgtcagcgta     1140

ttctccaaaa aagaatactg acaaaatagc cacggatagt tctttttttt ctcatatcag     1200

acatgctttt accgaaccta ttgttattgc ccctattctg gctcttatct gtgtcagtct     1260

ctctattcct ttccctgaaa ccttaaaatc ttctttaatg ctactaggaa aagcgaccgg     1320

aggcgttgcg cttttctctt ctggtataat tttattttct cgaaaagtta ttttaagtaa     1380

aacagtagca tctttagttt tatcaaaaaa tattattatt ccaacagcgg tattggttct     1440

tgcgtc                                                                1446


<210>  19
<211>  1661
<212>  DNA
<213>  artificial sequence

<220>
<223>  constructed fragment

<400>  19
gttcgatcaa caacccgaat cctatcgtaa tgatgttttg cccgatcagc ctcaatcgac       60

aattttacgc gtttcgatcg aagcagggac gacaattggc tgggaacggt atacttgaat      120

aaatggtctt cgttatggta ttgatgtttt tggtgcatcg gccccggcga atgatctata      180

tgctcatttc ggcttgaccg cagtcggcat cacgaacaag gtgttggccg cgatcgccgg      240

taagtcggca cgttaaaaaa tagctatgga atataatagc tactaataag ttaggagaat      300

aaacatgagt gtccaagcta cacgcgaaga taaatttagc tttggcttgt ggacagttgg      360

gtggcaggcc cgtgacgcct ttggtgatgc cacgcgtacc gcccttgatc cggtcgaagc      420

agttcataaa cttgccgaaa tcggagccta tggcattaca tttcatgatg atgacttggt      480

ccccttcggt tcggatgctc agacacggga tggtatcatt gctggtttca aaaaagccct      540

tgatgaaacc ggtttgatcg ttcctatggt taccacaaat ttgtttacgc atccggtttt      600

taaagacggc gggtttacct ctaacgatcg ttctgtgcgc cggtatgcta tccggaaagt      660

tttgagacag atggatcttg gtgcggaact gggggccaaa acgcttgtct tatggggcgg      720

cagagaaggt gcggaatatg attcagcaaa agatgttagt gccgccttgg atcgttatcg      780

cgaagcactg aatcttctgg cacaatatag cgaagacaga ggctatggac ttcgttttgc      840

aattgaaccg aaacctaatg aaccacgtgg cgatattctg ctgcctaccg caggccatgc      900

tattgccttt gtgcaagaat tagaacggcc cgaacttttt ggcatcaatc cagaaaccgg      960

gcatgaacag atgtcaaact tgaattttac ccaggggatt gctcaggctt tgtggcataa     1020

aaaacttttt catattgatt tgaatggaca acatggtccc aagtttgatc aggaccttgt     1080

ctttggtcat ggtgaccttt taaatgcctt tagcctggtc gatttgttag aaaatggtcc     1140

agatggtgcc ccggcttatg atggcccgcg ccattttgat tataaaccat ctcgtactga     1200

agattatgat ggcgtttggg aatcagcgaa agccaatatc cgtatgtatc ttttattaaa     1260

agaacgtgcc aaagcgttta gagctgatcc ggaagttcag gaagcactgg cagcaagcaa     1320

agttgccgaa ttgaaaaccc caacgttgaa tcctggtgaa ggctatgcag aactgttagc     1380

agatcgcagt gcttttgaag attatgatgc cgatgctgtt ggtgcgaaag gttttggttt     1440

cgtgaaattg aaccaattgg ccattgaaca tttattaggt gcccgctagt ctagatgact     1500

gtataaaacc acagccaatc aaacgaaacc aggctatact caagcctggt tttttgatgg     1560

attttcagcg tggcgcaggc aggttttatc ttaacccgac actggcggga caccccgcaa     1620

gggacagaag tctccttctg gctggcgacg gacaacgggc c                         1661


<210>  20
<211>  1960
<212>  DNA
<213>  artificial sequence

<220>
<223>  constructed fragment containing a 191 bp Peno, a 1,455-bp E. coli
       xylB coding sequence (ECxylB) and a 314-bp E.coli xylB 3'UTR

<400>  20
ccagttactc aatacgtaac aataatcagt ttatcctaac tatagaatcg catgagaagc       60

gataacgttt caccataagc aatatattca ttgcaacagt ggaattgcct tatgcgtcaa      120

ggaaggatag atcattgacg gactgagttc aaaaagagac tggtctaaaa gattttaaga      180

aaggtttcga tatgtatatc gggatagatc ttggcacctc gggcgtaaaa gttattttgc      240

tcaacgagca gggtgaggtg gttgctgcgc aaacggaaaa gctgaccgtt tcgcgcccgc      300

atccactctg gtcggaacaa gacccggaac agtggtggca ggcaactgat cgcgcaatga      360

aagctctggg cgatcagcat tctctgcagg acgttaaagc attgggtatt gccggccaga      420

tgcacggagc aaccttgctg gatgctcagc aacgggtgtt acgccctgcc attttgtgga      480

acgacgggcg ctgtgcgcaa gagtgcactt tgctggaagc gcgagttccg caatcgcggg      540

tgattaccgg caacctgatg atgcccggat ttactgcgcc taaattgcta tgggttcagc      600

ggcatgagcc ggagatattc cgtcaaatcg acaaagtatt attaccgaaa gattacttgc      660

gtctgcgtat gacgggggag tttgccagcg atatgtctga cgcagctggc accatgtggc      720

tggatgtcgc aaagcgtgac tggagtgacg tcatgctgca ggcttgcgac ttatctcgtg      780

accagatgcc cgcattatac gaaggcagcg aaattactgg tgctttgtta cctgaagttg      840

cgaaagcgtg gggtatggcg acggtgccag ttgtcgcagg cggtggcgac aatgcagctg      900

gtgcagttgg tgtgggaatg gttgatgcta atcaggcaat gttatcgctg gggacgtcgg      960

gggtctattt tgctgtcagc gaagggttct taagcaagcc agaaagcgcc gtacatagct     1020

tttgccatgc gctaccgcaa cgttggcatt taatgtctgt gatgctgagt gcagcgtcgt     1080

gtctggattg ggccgcgaaa ttaaccggcc tgagcaatgt cccagcttta atcgctgcag     1140

ctcaacaggc tgatgaaagt gccgagccag tttggtttct gccttatctt tccggcgagc     1200

gtacgccaca caataatccc caggcgaagg gggttttctt tggtttgact catcaacatg     1260

gccccaatga actggcgcga gcagtgctgg aaggcgtggg ttatgcgctg gcagatggca     1320

tggatgtcgt gcatgcctgc ggtattaaac cgcaaagtgt tacgttgatt gggggcgggg     1380

cgcgtagtga gtactggcgt cagatgctgg cggatatcag cggtcagcag ctcgattacc     1440

gtacgggggg ggatgtgggg ccagcactgg gcgcagcaag gctggcgcag atcgcggcga     1500

atccagagaa atcgctcatt gaattgttgc cgcaactacc gttagaacag tcgcatctac     1560

cagatgcgca gcgttatgcc gcttatcagc cacgacgaga aacgttccgt cgcctctatc     1620

agcaacttct gccattaatg gcgtaaacgt tatcccctgc ctgaccgggt gggggataat     1680

tcacatctat atatctcagt aattaattaa tatttagtac gaatttattc tgaaaatcat     1740

ttgttaatgg catttttcag ttttgtcttt cgttggttac tcgtaatgta tcgctggtag     1800

atatggagat cgttatgaaa acctcaaaga ctgtggcaaa actattattt gttgtcgggg     1860

cgctggttta tctggttggg ctatggatct catgcccatt gttaagtgga aaaggctatt     1920

ttcttggcgt gttaatgaca gcaacttttg gcaactatgc                           1960


<210>  21
<211>  1014
<212>  DNA
<213>  artificial sequence

<220>
<223>  constructed fragment with spec resistance gene bounded by lox 
       recombination sites

<400>  21
agcacaggat gacgcctaac aattcattca agccgacacc gcttcgcggc gcggcttaat       60

tcaggagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga      120

ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg      180

ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac      240

tgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc      300

ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga      360

catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa      420

tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct      480

gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga      540

tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc      600

gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta      660

cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg      720

cctgccggcc cagtatcagc ccgtcatact tgaagctagg caggcttatc ttggacaaga      780

agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gttcactacg tgaaaggcga      840

gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgac gccgcttcgc      900

ggcgcggctt aactcaagcg ttagagagct ggggaagact atgcgcgatc tgttgaaggt      960

ggttctaagc ctcgtacttg cgatggcatc ggggcaggca cttgctgacc tgcc           1014


<210>  22
<211>  12198
<212>  DNA
<213>  artificial sequence

<220>
<223>  constructed plasmid

<400>  22
ctagtgttcg atcaacaacc cgaatcctat cgtaatgatg ttttgcccga tcagcctcaa       60

tcgacaattt tacgcgtttc gatcgaaaca gggacgacaa ttggctggga acggtatact      120

ggaataaatg gtcttcgtta tggtattgat gtttttggtg catcggcccc ggcgaatgat      180

ctatatgctc atttcggctt gaccgcagtc ggcatcacga acaaggtgtt ggccgcgatc      240

gccggtaagt cggcacgtta aaaaatagct atggaatata atagctacta ataagttagg      300

agaataaaca tgacggacaa attgacctcc cttcgtcagt acaccaccgt agtggccgac      360

actggggaca tcgcggcaat gaagctgtat caaccgcagg atgccacaac caacccttct      420

ctcattctta acgcagcgca gattccggaa taccgtaagt tgattgatga tgctgtcgcc      480

tgggcgaaac agcagagcaa cgatcgcgcg cagcagatcg tggacgcgac cgacaaactg      540

gcagtaaata ttggtctgga aatcctgaaa ctggttccgg gccgtatctc aactgaagtt      600

gatgcgcgcc tttcctatga caccgaagcg tcaattgcga aagcaaaacg cctgatcaaa      660

ctctacaacg atgctggtat tagcaacgat cgtattctga tcaaactggc ttctacctgg      720

cagggtatcc gtgctgcaga acagctggaa aaagaaggca tcaactgtaa cctgaccctg      780

ctgttctcct tcgctcaggc tcgtgcttgt gcggaagcgg gcgtgttcct gatctcgccg      840

tttgttggcc gtattcttga ctggtacaaa gcgaataccg ataagaaaga gtacgctccg      900

gcagaagatc cgggcgtggt ttctgtatct gaaatctacc agtactacaa agagcacggt      960

tatgaaaccg tggttatggg cgcaagcttc cgtaacatcg gcgaaattct ggaactggca     1020

ggctgcgacc gtctgaccat cgcaccggca ctgctgaaag agctggcgga gagcgaaggg     1080

gctatcgaac gtaaactgtc ttacaccggc gaagtgaaag cgcgtccggc gcgtatcact     1140

gagtccgagt tcctgtggca gcacaaccag ggtccaatgg cagtagataa actggcggaa     1200

ggtatccgta agtttgctat tgaccaggaa aaactggaaa aaatgatcgg cgatctgctg     1260

taatctagac gatctggagt caaaatgtcc tcacgtaaag agcttgccaa tgctattcgt     1320

gcgctgagca tggacgcagt acagaaagcc aaatccggtc acccgggtgc ccctatgggt     1380

atggctgaca ttgccgaagt cctgtggcgt gatttcctga aacacaaccc gcagaatccg     1440

tcctgggctg accgtgaccg cttcgtgctg tccaacggcc acggctccat gctgatctac     1500

agcctgctgc acctcaccgg ttacgatctg ccgatggaag aactgaaaaa cttccgtcag     1560

ctgcactcta aaactccggg tcacccggaa gtgggttaca ccgctggtgt ggaaaccacc     1620

accggtccgc tgggtcaggg tattgccaac gcagtcggta tggcgattgc agaaaaaacg     1680

ctggcggcgc agtttaaccg tccgggccac gacattgtcg accactacac ctacgccttc     1740

atgggcgacg gctgcatgat ggaaggcatc tcccacgaag tttgctctct ggcgggtacg     1800

ctgaagctgg gtaaactgat tgcattctac gatgacaacg gtatttctat cgatggtcac     1860

gttgaaggct ggttcaccga cgacaccgca atgcgtttcg aagcttacgg ctggcacgtt     1920

attcgcgaca tcgacggtca tgacgcggca tctatcaaac gcgcagtaga agaagcgcgc     1980

gcagtgactg acaaaccttc cctgctgatg tgcaaaacca tcatcggttt cggttccccg     2040

aacaaagccg gtacccacga ctcccacggt gcgccgctgg gcgacgctga aattgccctg     2100

acccgcgaac aactgggctg gaaatatgcg ccgttcgaaa tcccgtctga aatctatgct     2160

cagtgggatg cgaaagaagc aggccaggcg aaagaatccg catggaacga gaaattcgct     2220

gcttacgcga aagcttatcc gcaggaagcc gctgaattta cccgccgtat gaaaggcgaa     2280

atgccgtctg acttcgacgc taaagcgaaa gagttcatcg ctaaactgca ggctaatccg     2340

gcgaaaatcg ccagccgtaa agcgtctcag aatgctatcg aagcgttcgg tccgctgttg     2400

ccggaattcc tcggcggttc tgctgacctg gcgccgtcta acctgaccct gtggtctggt     2460

tctaaagcaa tcaacgaaga tgctgcgggt aactacatcc actacggtgt tcgcgagttc     2520

ggtatgaccg cgattgctaa cggtatctcc ctgcacggtg gcttcctgcc gtacacctcc     2580

accttcctga tgttcgtgga atacgcacgt aacgccgtac gtatggctgc gctgatgaaa     2640

cagcgtcagg tgatggttta cacccacgac tccatcggtc tgggcgaaga cggcccgact     2700

caccagccgg ttgagcaggt cgcttctctg cgcgtaaccc cgaacatgtc tacatggcgt     2760

ccgtgtgacc aggttgaatc cgcggtcgcg tggaaatacg gtgttgagcg tcaggacggc     2820

ccgaccgcac tgatcctctc ccgtcagaac ctggcgcagc aggaacgaac tgaagagcaa     2880

ctggcaaaca tcgcgcgcgg tggttatgtg ctgaaagact gcgccggtca gccggaactg     2940

attttcatcg ctaccggttc agaagttgaa ctggctgttg ctgcctacga aaaactgact     3000

gccgaaggcg tgaaagcgcg cgtggtgtcc atgccgtcta ccgacgcatt tgacaagcag     3060

gatgctgctt accgtgaatc cgtactgccg aaagcggtta ctgcacgcgt tgctgtagaa     3120

gcgggtattg ctgactactg gtacaagtat gttggcctga acggtgctat cgtcggtatg     3180

accaccttcg gtgaatctgc tccggcagag ctgctgtttg aagagttcgg cttcactgtt     3240

gataacgttg ttgcgaaagc aaaagaactg ctgtaattag catttcgggt aaaaaaggtc     3300

gcttcggcga ccttttttat taccttgata atgtccgttt gcgcggcgcg ccccagttac     3360

tcaatacgta acaataatca gtttatccta actatagaat cgcatgagaa gcgataacgt     3420

ttcaccataa gcaatatatt cattgcaaca gtggaattgc cttatgcgtc aaggaaggat     3480

agatcattga cggactgagt tcaaaaagag actggtctaa aagattttaa gaaaggtttc     3540

gatatgacct ctgctgtgcc atcaaatacg aaaaaaaagc tggtgattgc ttccgatcac     3600

gcagcatttg agttgaaatc aaccttgatt acttggctga aagagcttgg tcatgaggtc     3660

gaagaccttg gccctcatga aaaccattca gtcgattatc ccgattacgg ttataagctg     3720

gctgtcgcta tcgcagaaaa aaccgctgat ttcggtattg ctttatgtgg ctcgggaatc     3780

ggtatctcga tcgctgtcaa tcgccatccg gctgcccgtt gcgctttgat tacggataac     3840

cttaccgccc gtttggcaag agaacataac aatgccaatg ttatcgctat gggtgcgaga     3900

ttgatcggca ttgaaaccgc taaggattgt atttcagctt tccttgcaac gccgtttgga     3960

ggtgaacgtc atgttcgccg tatcgataaa ctttcgaatc ctcagttcaa tatctagata     4020

agttaggaga ataaacatga gtaaattacc cctgattgct ccctctatcc tttcggcgga     4080

ttttgcccat ttgggagatg aggtcgcggc gatagatcag gccggtgccg attggatcca     4140

tattgatgtg atggatggcc atttcgtgcc gaatatcacc ataggcccca tggttgtgaa     4200

ggctttgcgt ccctatagcc aaaagccttt tgatgtccat ttgatgattg cgcctgtcga     4260

tcaatatatc gaggcttttt ctgaagcggg tgctgatatt atcagtttcc atcccgaagc     4320

gggcgcgcat ccccatcgca ctattcagca tatcaaatca ttgggcaaaa aagcgggatt     4380

agtttttaat ccggcgaccc ctttaagctg gcttgattat ctaatggatg atcttgatct     4440

gattatggtg atgagcgtta accccggttt tggcggccaa aaatttatca aaacccaatt     4500

agaaaagatt aaagatatcc gtcaaagaat taccgcctct gggcgggata tccgcttgga     4560

agtggatggc ggaattgatg ccacgactgc accgcttgcc gtcgaagccg gtgccgatgt     4620

tttggtcgcg ggaacggcca gctttaaagg cggcgcaaca tgttacaccg ataatatcag     4680

gatattgcgt aaatcatgat taattaactc gaggcggcct gaacgtactg caagtcctga     4740

cgtcactgtg cagtccgttg gcccggttat cggtagcgat accgggcatt tttttaagga     4800

acgatcgata gaattcgcgg gccggccaag cttgaattca tggttttggt gccaatgtta     4860

tcgcctataa accgcatcca gaccccgaat tggcgaaaaa ggtcggtttc cgcttcacct     4920

ctctcgatga agtgatcgag accagcgaca tcatttcgct tcactgtccg ctcacgccag     4980

aaaatcatca catgattaat gaagaaacac tggcaagggc aaaaaaaggc ttttacctcg     5040

tcaataccag tcgcggcggc ttggttgata ccaaggcggt gattaaatcg ctgaaagcca     5100

aacatctcgg cggttatgcg gcggatgttt acgaagagga ggggccttta ttcttcgaaa     5160

atcacgctga cgatattatc gaagatgata ttctcgaaag gttgatcgct ttcccgaatg     5220

tggttttcac gggacatcag gcctttttga cgaaagaggc cttatcaaac attgctcaca     5280

gtattctaca agatatcagc gatgccgaag ctggaaaaga aatgccggat gcgcttgttt     5340

agtagacaag cgacaattaa ccttttgaag atcataatga tcaaattttt gggttaattc     5400

ggtagttatg gcataggcta ttacgcgcta attgatatca aaaaaaagca tagccggaca     5460

tcataccggc tatgtttttt attaggaaaa aatttccttt caccttgctt agccatcgcc     5520

gcattattta atcaatatgc cgagtttttc ttgaaatccc tatcttacac caaggccaac     5580

aagggaatca tccatactcg gtgtcctatc ctatgacttt ttaaattttc tccaaattta     5640

ctaaaatcac gccatctcag cggctgctat tttcaaaaag cgcctctcaa aaccgctttt     5700

tcctgctcaa atatcggatc ccaaaattcc ctcaaaaaag gcagggtatt ttttacaaaa     5760

tcgcccctaa tatctctcaa tccgctgcct tgttcatatg tttttgcaaa tgatttttat     5820

taaacttttt taggcgtatt tttatcaaga aaatttaaat aatcacattt ttattatttt     5880

agatttaagt attgatacaa gtgatatcta taaatgtttt tataactttc tggatcgtaa     5940

tcggctggca atcgttttcc ctatattcgc aagatgtatg tcagccgcgc ggccgcctaa     6000

ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg     6060

cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata     6120

ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat     6180

atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa     6240

tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga     6300

acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt     6360

atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta     6420

ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg atttagtgta tgatggtgtt     6480

tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg     6540

gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctagcggagt gtatactggc     6600

ttactatgtt ggcactgatg agggtgtcag tgaagtgctt catgtggcag gagaaaaaag     6660

gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc ctcgctcact     6720

gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga     6780

gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc     6840

cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt     6900

ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg     6960

tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt     7020

tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt     7080

atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag     7140

tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga     7200

ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga     7260

ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga     7320

aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa     7380

acgatctcaa gaagatcatc ttattaatca gataaaatat ttctagattt cagtgcaatt     7440

tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt aattctcatg     7500

tttgacagct tatcatcgat gtgacggaag atcacttcgc agaataaata aatcctggtg     7560

tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac gttgatcggc     7620

acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg     7680

agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata     7740

ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg     7800

ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa     7860

agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg     7920

ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc     7980

acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat     8040

accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg     8100

aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc     8160

cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc     8220

ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga     8280

ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac     8340

aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc     8400

cttaaacgcc tggttgctac gcctgaataa gtcgaccttt gtagtcttgg cctgttgtgt     8460

gcatgagcaa atcaatggca ccaccccctc ctttttgagc tgaatggtca taaaatttat     8520

aattatctat cgtaattcgg aatctatgtt cagggtctcg ccattgcttt ttgtctgctg     8580

ggtcaagttc catgcctaag gtttttaaga catcagaaag aggtattgca cgcatgctat     8640

cagcttttct tctagctaat gacagggctt cctctgctct atctgctcgt tttttttctt     8700

ccacatatct cgccgctttg tcagccagcg gctgtattac ggaaagtgcc gatttttggg     8760

cttttaggcg ttctttttct gcccattctt ccttatttgt aaaaattgag ggtgggatgg     8820

gtgcctgaat cttgggatct agctgtaaag ttttgttgat atttccgtaa tgtctttgga     8880

ctctttgatg cgttgctttt gaacctttta cgcctctggc cagccctaga ggctccatag     8940

aagccgcata atccgtctgg agggcagaaa gggcttttcg accatcaaac catctcgatg     9000

cgtttaaacg gcctgtatcg gggtctctag gcaccataaa gccggttaag tggggtgttg     9060

tttcatcagc atgtagctga agagatacaa ggttgttttc tccaaaggtt tgttccgccc     9120

attgctgggt gattgttttc cagtgttcga gtttttcagg agtggcctgt tttgaccatt     9180

ctggagacat accaaagaac agttctatgg cctgcacacc gttttttcta agaggctttc     9240

ccgtttcttt ctgaatttta ttcagcatag atttaacatc tgctgatggg tcagtagagc     9300

ctttgagtat ttcgtttagt tcttttctat ctgggtcagc gttttgtgtt tcgcggcctc     9360

gcgtcatatg caggctcgcg gctttaatcg tgccaactgt tttatgtttt tcaaacctaa     9420

agattgcata gttcggcatg ttttaactgc tttaatttga gaaaagacca gaggaaataa     9480

tccagcctat atttctttcc ctagtagcga actggaattg tttttccgaa ggaaaaaagc     9540

aattccgtag tgagtactga atttattctg attcgtcttg cttttggagc gtctttttgc     9600

gttctataac tgttgtgaaa gctacgcggt cgccattgaa aacgaaatta ggattaataa     9660

aataccatcc ttggcgaaca tgctttgcaa tgattttagc tttttctaat tcggctagac     9720

ctcttgcaaa ggtagcttga gatagtgcca gttttttttc ttgtgcgtta agaaagtcct     9780

ctaaaacgaa tttgtctaaa gggacgaggt ctttgctgat gcctttgtct tgaagtatcc     9840

aaaccagaac gctgaaagct tttattccag cggctcctag ttcaaaagtt agcgcgatat     9900

tggtgctaaa taattttaca aattcttcac tatcaacacg tctgtaagtc gtcacatgag     9960

tgccttgcat ctcaccagtg gcttgattga ccagaatgtt atcatctcgt cctaatcgag    10020

ataactgaac cctctgactt ttaactggca caaccatacc ttcgatgaaa ggattctcgt    10080

catatctgat tggctgcttt ctcaattttg tcgccatatt tgataaacct ttaatcaaaa    10140

aaaccacatt ttttgattat acctattcat cgaatgaggc aaggtctatc aattttaccc    10200

ctttttttga tagacggttt aatcaatatt gatagacccc ttcacagatt ctgaaaatcg    10260

acttccctat tttagggata ttttcacgat tccctttctt agttcttcct agtggggaaa    10320

ttcgttgaat cctgcctcgg aaaaaccatg agaaagctgt tggttatata cacgggcaaa    10380

gccaccctat ttttagctac tggggaaaga gataaggcag ggtatttgta aaattaaaac    10440

cggatttttc gctttacggt ttgtttaggc gcaactgtct ttttaagacc gcgtttaacc    10500

atcaaaagat cgttccaatc ttttccgtgt atcatctgtt ctttaggtgg gagccagttt    10560

tcaacttttt ttgttggaaa cgcggcttta atcgctccga ctaatagcga tgctgctctt    10620

tgtcctacag catcccaatc ataggcaata tggacagaag atgccttttc aacgattttt    10680

cggagagttt tagtaagaga cgttcttacg ccgctggtgc ttaataattt tacgccagct    10740

ttaatttttt ctgggcttaa aaagccgact actgaaatcg cgtctatcgc actttcagcg    10800

atataaagat catacttttc gtcatttttt acattgatgc tgccagtaaa atgggcttcg    10860

cgactgcttc ccaaggctaa ccctttaaaa ccactgcttg ttccgcgtaa ttctgcgccc    10920

tgaagtgtat ctttatcgtc atacatcaag aaggctacat taccgcgatc atctgttcgg    10980

atagagtcag gaatattgtt aaatgatatt cctcgggcag cgttgggtcc tggccacggg    11040

tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg    11100

gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt    11160

ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa    11220

cgcggaagtc ccctacgtgc tgctgaagtt gcccgcaaca gagagtggaa ccaaccggtg    11280

ataccacgat actatgactg agagtcaacg ccatgggagc tcgtttttct atccccatca    11340

cctcggtttt gttgacaaaa aaaggtggcc actaaattgg ctttccgcac cgatgggatg    11400

atttttattc tttgctattc ttcgctcttt gcccaattca ttaaaagcgg aaatcatcac    11460

caaagataga agacgcagcc ttcaccattt cagattgccc ttctcgggca ttttctgctg    11520

ctagaatcct cttaaaaata ttaaattcca ctctattggt aatatgtttc cctctttagg    11580

gaacaaataa agcccttctt tgttctataa aagttagctt accgatttta caaaaaataa    11640

taccgcttca ttcaatcggt aatacatatc ttttttcttc aaaaaacttt tcaagagggt    11700

gtctatgcgc gtcgcaatat tcagttccaa aaactatgac catcattcta ttgaaaaaga    11760

aaatgaacat tatggccatg accttgtttt tctgaatgag cggcttacca aagagacagc    11820

agaaaaagcc aaagacgcag aagctgtttg tatctttgtg aatgacgaag ccaatgccga    11880

agtgctggaa attttggcag gcttaggcat caagttggtt gctcttcgtt gcgccggtta    11940

taacaatgtc gatctcgatg cggccaaaaa gctgaatatc aaggttgtgc gcgtgcctgc    12000

ctattcgccc tattcggttg ccgaatatgc agtagggatg ttgctcaccc tgaatcggca    12060

aatttcacgc ggtttgaagc gggttcggga aaataacttc tccttggaag gtttgattgg    12120

ccttgatgtg catgacaaaa cagtcggcat tatcggtgtt ggtcatatcg ggagcgtctt    12180

tgcccatatt atgaccca                                                  12198


<210>  23
<211>  875
<212>  DNA
<213>  artificial sequence

<220>
<223>  constructed fragment for targeting integration into the ldh locus
       of Z. mobilis

<400>  23
gtttttctat ccccatcacc tcggttttgt tgacaaaaaa aggtggccac taaattggct       60

ttccgcaccg atgggatgat ttttattctt tgctattctt cgctctttgc ccaattcatt      120

aaaagcggaa atcatcacca aagatagaag acgcagcctt caccatttca gattgccctt      180

ctcgggcatt ttctgctgct agaatcctct taaaaatatt aaattccact ctattggtaa      240

tatgtttccc tctttaggga acaaataaag cccttctttg ttctataaaa gttagcttac      300

cgattttaca aaaaataata ccgcttcatt caatcggtaa tacatatctt ttttcttcaa      360

aaaacttttc aagagggtgt ctatgcgcgt cgcaatattc agttccaaaa actatgacca      420

tcattctatt gaaaaagaaa atgaacatta tggccatgac cttgtttttc tgaatgagcg      480

gcttaccaaa gagacagcag aaaaagccaa agacgcagaa gctgtttgta tctttgtgaa      540

tgacgaagcc aatgccgaag tgctggaaat tttggcaggc ttaggcatca agttggttgc      600

tcttcgttgc gccggttata acaatgtcga tctcgatgcg gccaaaaagc tgaatatcaa      660

ggttgtgcgc gtgcctgcct attcgcccta ttcggttgcc gaatatgcag tagggatgtt      720

gctcaccctg aatcggcaaa tttcacgcgg tttgaagcgg gttcgggaaa ataacttctc      780

cttggaaggt ttgattggcc ttgatgtgca tgacaaaaca gtcggcatta tcggtgttgg      840

tcatatcggg agcgtctttg cccatattat gaccc                                 875


<210>  24
<211>  1149
<212>  DNA
<213>  artificial sequence

<220>
<223>  constructed fragment for integration into the Z. mobilis ldh 
       locus

<400>  24
atggttttgg tgccaatgtt atcgcctata aaccgcatcc agaccccgaa ttggcgaaaa       60

aggtcggttt ccgcttcacc tctctcgatg aagtgatcga gaccagcgac atcatttcgc      120

ttcactgtcc gctcacgcca gaaaatcatc acatgattaa tgaagaaaca ctggcaaggg      180

caaaaaaagg cttttacctc gtcaatacca gtcgcggcgg cttggttgat accaaggcgg      240

tgattaaatc gctgaaagcc aaacatctcg gcggttatgc ggcggatgtt tacgaagagg      300

aggggccttt attcttcgaa aatcacgctg acgatattat cgaagatgat attctcgaaa      360

ggttgatcgc tttcccgaat gtggttttca cgggacatca ggcctttttg acgaaagagg      420

ccttatcaaa cattgctcac agtattctac aagatatcag cgatgccgaa gctggaaaag      480

aaatgccgga tgcgcttgtt tagtagacaa gcgacaatta accttttgaa gatcataatg      540

atcaaatttt tgggttaatt cggtagttat ggcataggct attacgcgct aattgatatc      600

aaaaaaaagc atagccggac atcataccgg ctatgttttt tattaggaaa aaatttcctt      660

tcaccttgct tagccatcgc cgcattattt aatcaatatg ccgagttttt cttgaaatcc      720

ctatcttaca ccaaggccaa caagggaatc atccatactc ggtgtcctat cctatgactt      780

tttaaatttt ctccaaattt actaaaatca cgccatctca gcggctgcta ttttcaaaaa      840

gcgcctctca aaaccgcttt ttcctgctca aatatcggat cccaaaattc cctcaaaaaa      900

ggcagggtat tttttacaaa atcgccccta atatctctca atccgctgcc ttgttcatat      960

gtttttgcaa atgattttta ttaaactttt ttaggcgtat ttttatcaag aaaatttaaa     1020

taatcacatt tttattattt tagatttaag tattgataca agtgatatct ataaatgttt     1080

ttataacttt ctggatcgta atcggctggc aatcgttttc cctatattcg caagatgtat     1140

gtcagccgc                                                             1149


<210>  25
<211>  996
<212>  DNA
<213>  Zymomonas mobilis

<400>  25
atgcgcgtcg caatattcag ttccaaaaac tatgaccatc attctattga aaaagaaaat       60

gaacattatg gccatgacct tgtttttctg aatgagcggc ttaccaaaga gacagcagaa      120

aaagccaaag acgcagaagc tgtttgtatc tttgtgaatg acgaagccaa tgccgaagtg      180

ctggaaattt tggcaggctt aggcatcaag ttggttgctc ttcgttgcgc cggttataac      240

aatgtcgatc tcgatgcggc caaaaagctg aatatcaagg ttgtgcgcgt gcctgcctat      300

tcgccctatt cggttgccga atatgcagta gggatgttgc tcaccctgaa tcggcaaatt      360

tcacgcggtt tgaagcgggt tcgggaaaat aacttctcct tggaaggttt gattggcctt      420

gatgtgcatg acaaaacagt cggcattatc ggtgttggtc atatcgggag cgtctttgcc      480

catattatga cccatggttt tggtgccaat gttatcgcct ataaaccgca tccagacccc      540

gaattggcga aaaaggtcgg tttccgcttc acctctctcg atgaagtgat cgagaccagc      600

gacatcattt cgcttcactg tccgctcacg ccagaaaatc atcacatgat taatgaagaa      660

acactggcaa gggcaaaaaa aggcttttac ctcgtcaata ccagtcgcgg cggcttggtt      720

gataccaagg cggtgattaa atcgctgaaa gccaaacatc tcggcggtta tgcggcggat      780

gtttacgaag aggaggggcc tttattcttc gaaaatcacg ctgacgatat tatcgaagat      840

gatattctcg aaaggttgat cgctttcccg aatgtggttt tcacgggaca tcaggccttt      900

ttgacgaaag aggccttatc aaacattgct cacagtattc tacaagatat cagcgatgcc      960

gaagctggaa aagaaatgcc ggatgcgctt gtttag                                996


<210>  26
<211>  3339
<212>  DNA
<213>  artificial sequence

<220>
<223>  constructed fragment containing a 304-bp T-mutant of the Z. 
       mobilis GAP promoter (PgapT), a 954-bp E. coli Tal coding region 
       (ECTal), a 1,992-bp E. coli Tkt coding region, and a 68-bp E. 
       coli Tkt 3'UTR (ECTkt 3'UTR)

<400>  26
gttcgatcaa caacccgaat cctatcgtaa tgatgttttg cccgatcagc ctcaatcgac       60

aattttacgc gtttcgatcg aaacagggac gacaattggc tgggaacggt atactggaat      120

aaatggtctt cgttatggta ttgatgtttt tggtgcatcg gccccggcga atgatctata      180

tgctcatttc ggcttgaccg cagtcggcat cacgaacaag gtgttggccg cgatcgccgg      240

taagtcggca cgttaaaaaa tagctatgga atataatagc tactaataag ttaggagaat      300

aaacatgacg gacaaattga cctcccttcg tcagtacacc accgtagtgg ccgacactgg      360

ggacatcgcg gcaatgaagc tgtatcaacc gcaggatgcc acaaccaacc cttctctcat      420

tcttaacgca gcgcagattc cggaataccg taagttgatt gatgatgctg tcgcctgggc      480

gaaacagcag agcaacgatc gcgcgcagca gatcgtggac gcgaccgaca aactggcagt      540

aaatattggt ctggaaatcc tgaaactggt tccgggccgt atctcaactg aagttgatgc      600

gcgcctttcc tatgacaccg aagcgtcaat tgcgaaagca aaacgcctga tcaaactcta      660

caacgatgct ggtattagca acgatcgtat tctgatcaaa ctggcttcta cctggcaggg      720

tatccgtgct gcagaacagc tggaaaaaga aggcatcaac tgtaacctga ccctgctgtt      780

ctccttcgct caggctcgtg cttgtgcgga agcgggcgtg ttcctgatct cgccgtttgt      840

tggccgtatt cttgactggt acaaagcgaa taccgataag aaagagtacg ctccggcaga      900

agatccgggc gtggtttctg tatctgaaat ctaccagtac tacaaagagc acggttatga      960

aaccgtggtt atgggcgcaa gcttccgtaa catcggcgaa attctggaac tggcaggctg     1020

cgaccgtctg accatcgcac cggcactgct gaaagagctg gcggagagcg aaggggctat     1080

cgaacgtaaa ctgtcttaca ccggcgaagt gaaagcgcgt ccggcgcgta tcactgagtc     1140

cgagttcctg tggcagcaca accagggtcc aatggcagta gataaactgg cggaaggtat     1200

ccgtaagttt gctattgacc aggaaaaact ggaaaaaatg atcggcgatc tgctgtaatc     1260

tagacgatct ggagtcaaaa tgtcctcacg taaagagctt gccaatgcta ttcgtgcgct     1320

gagcatggac gcagtacaga aagccaaatc cggtcacccg ggtgccccta tgggtatggc     1380

tgacattgcc gaagtcctgt ggcgtgattt cctgaaacac aacccgcaga atccgtcctg     1440

ggctgaccgt gaccgcttcg tgctgtccaa cggccacggc tccatgctga tctacagcct     1500

gctgcacctc accggttacg atctgccgat ggaagaactg aaaaacttcc gtcagctgca     1560

ctctaaaact ccgggtcacc cggaagtggg ttacaccgct ggtgtggaaa ccaccaccgg     1620

tccgctgggt cagggtattg ccaacgcagt cggtatggcg attgcagaaa aaacgctggc     1680

ggcgcagttt aaccgtccgg gccacgacat tgtcgaccac tacacctacg ccttcatggg     1740

cgacggctgc atgatggaag gcatctccca cgaagtttgc tctctggcgg gtacgctgaa     1800

gctgggtaaa ctgattgcat tctacgatga caacggtatt tctatcgatg gtcacgttga     1860

aggctggttc accgacgaca ccgcaatgcg tttcgaagct tacggctggc acgttattcg     1920

cgacatcgac ggtcatgacg cggcatctat caaacgcgca gtagaagaag cgcgcgcagt     1980

gactgacaaa ccttccctgc tgatgtgcaa aaccatcatc ggtttcggtt ccccgaacaa     2040

agccggtacc cacgactccc acggtgcgcc gctgggcgac gctgaaattg ccctgacccg     2100

cgaacaactg ggctggaaat atgcgccgtt cgaaatcccg tctgaaatct atgctcagtg     2160

ggatgcgaaa gaagcaggcc aggcgaaaga atccgcatgg aacgagaaat tcgctgctta     2220

cgcgaaagct tatccgcagg aagccgctga atttacccgc cgtatgaaag gcgaaatgcc     2280

gtctgacttc gacgctaaag cgaaagagtt catcgctaaa ctgcaggcta atccggcgaa     2340

aatcgccagc cgtaaagcgt ctcagaatgc tatcgaagcg ttcggtccgc tgttgccgga     2400

attcctcggc ggttctgctg acctggcgcc gtctaacctg accctgtggt ctggttctaa     2460

agcaatcaac gaagatgctg cgggtaacta catccactac ggtgttcgcg agttcggtat     2520

gaccgcgatt gctaacggta tctccctgca cggtggcttc ctgccgtaca cctccacctt     2580

cctgatgttc gtggaatacg cacgtaacgc cgtacgtatg gctgcgctga tgaaacagcg     2640

tcaggtgatg gtttacaccc acgactccat cggtctgggc gaagacggcc cgactcacca     2700

gccggttgag caggtcgctt ctctgcgcgt aaccccgaac atgtctacat ggcgtccgtg     2760

tgaccaggtt gaatccgcgg tcgcgtggaa atacggtgtt gagcgtcagg acggcccgac     2820

cgcactgatc ctctcccgtc agaacctggc gcagcaggaa cgaactgaag agcaactggc     2880

aaacatcgcg cgcggtggtt atgtgctgaa agactgcgcc ggtcagccgg aactgatttt     2940

catcgctacc ggttcagaag ttgaactggc tgttgctgcc tacgaaaaac tgactgccga     3000

aggcgtgaaa gcgcgcgtgg tgtccatgcc gtctaccgac gcatttgaca agcaggatgc     3060

tgcttaccgt gaatccgtac tgccgaaagc ggttactgca cgcgttgctg tagaagcggg     3120

tattgctgac tactggtaca agtatgttgg cctgaacggt gctatcgtcg gtatgaccac     3180

cttcggtgaa tctgctccgg cagagctgct gtttgaagag ttcggcttca ctgttgataa     3240

cgttgttgcg aaagcaaaag aactgctgta attagcattt cgggtaaaaa aggtcgcttc     3300

ggcgaccttt tttattacct tgataatgtc cgtttgcgc                            3339


<210>  27
<211>  304
<212>  DNA
<213>  artificial sequence

<220>
<223>  mutant Pgap promoter with a "G" to an "A" change at position 83 
       in SEQ ID NO:21

<400>  27
gttcgatcaa caacccgaat cctatcgtaa tgatgttttg cccgatcagc ctcaatcgac       60

aattttacgc gtttcgatcg aaacagggac gacaattggc tgggaacggt atactggaat      120

aaatggtctt cgttatggta ttgatgtttt tggtgcatcg gccccggcga atgatctata      180

tgctcatttc ggcttgaccg cagtcggcat cacgaacaag gtgttggccg cgatcgccgg      240

taagtcggca cgttaaaaaa tagctatgga atataatagc tactaataag ttaggagaat      300

aaac                                                                   304


<210>  28
<211>  1443
<212>  DNA
<213>  artificial sequence

<220>
<223>  constructed fragment containing a 191 bp Peno, a 471 bp Z. 
       mobilis Rpi coding sequence (ZMRpi), a 663 bp Z. mobilis Rpe 
       coding sequence (ZMRpe), and a 35 bp E.coli xylA 3'UTR (ECxylA 
       3'UTR)

<400>  28
ccagttactc aatacgtaac aataatcagt ttatcctaac tatagaatcg catgagaagc       60

gataacgttt caccataagc aatatattca ttgcaacagt ggaattgcct tatgcgtcaa      120

ggaaggatag atcattgacg gactgagttc aaaaagagac tggtctaaaa gattttaaga      180

aaggtttcga tatgacctct gctgtgccat caaatacgaa aaaaaagctg gtgattgctt      240

ccgatcacgc agcatttgag ttgaaatcaa ccttgattac ttggctgaaa gagcttggtc      300

atgaggtcga agaccttggc cctcatgaaa accattcagt cgattatccc gattacggtt      360

ataagctggc tgtcgctatc gcagaaaaaa ccgctgattt cggtattgct ttatgtggct      420

cgggaatcgg tatctcgatc gctgtcaatc gccatccggc tgcccgttgc gctttgatta      480

cggataacct taccgcccgt ttggcaagag aacataacaa tgccaatgtt atcgctatgg      540

gtgcgagatt gatcggcatt gaaaccgcta aggattgtat ttcagctttc cttgcaacgc      600

cgtttggagg tgaacgtcat gttcgccgta tcgataaact ttcgaatcct cagttcaata      660

tctagataag ttaggagaat aaacatgagt aaattacccc tgattgctcc ctctatcctt      720

tcggcggatt ttgcccattt gggagatgag gtcgcggcga tagatcaggc cggtgccgat      780

tggatccata ttgatgtgat ggatggccat ttcgtgccga atatcaccat aggccccatg      840

gttgtgaagg ctttgcgtcc ctatagccaa aagccttttg atgtccattt gatgattgcg      900

cctgtcgatc aatatatcga ggctttttct gaagcgggtg ctgatattat cagtttccat      960

cccgaagcgg gcgcgcatcc ccatcgcact attcagcata tcaaatcatt gggcaaaaaa     1020

gcgggattag tttttaatcc ggcgacccct ttaagctggc ttgattatct aatggatgat     1080

cttgatctga ttatggtgat gagcgttaac cccggttttg gcggccaaaa atttatcaaa     1140

acccaattag aaaagattaa agatatccgt caaagaatta ccgcctctgg gcgggatatc     1200

cgcttggaag tggatggcgg aattgatgcc acgactgcac cgcttgccgt cgaagccggt     1260

gccgatgttt tggtcgcggg aacggccagc tttaaaggcg gcgcaacatg ttacaccgat     1320

aatatcagga tattgcgtaa atcatgatta attaactcga ggcggcctga acgtactgca     1380

agtcctgacg tcactgtgca gtccgttggc ccggttatcg gtagcgatac cgggcatttt     1440

ttt                                                                   1443


<210>  29
<211>  12704
<212>  DNA
<213>  artificial sequence

<220>
<223>  constructed plasmid

<400>  29
ctagtgttcg atcaacaacc cgaatcctat cgtaatgatg ttttgcccga tcagcctcaa       60

tcgacaattt tacgcgtttc gatcgaagca gggacgacaa ttggctggga acggtatact      120

ggaataaatg gtcttcgtta tggtattgat gtttttggtg catcggcccc ggcgaatgat      180

ctatatgctc atttcggctt gaccgcagtc ggcatcacga acaaggtgtt ggccgcgatc      240

gccggtaagt cggcacgtta aaaaatagct atggaatata atagctacta ataagttagg      300

agaataaaca tgacggacaa attgacctcc cttcgtcagt acaccaccgt agtggccgac      360

actggggaca tcgcggcaat gaagctgtat caaccgcagg atgccacaac caacccttct      420

ctcattctta acgcagcgca gattccggaa taccgtaagt tgattgatga tgctgtcgcc      480

tgggcgaaac agcagagcaa cgatcgcgcg cagcagatcg tggacgcgac cgacaaactg      540

gcagtaaata ttggtctgga aatcctgaaa ctggttccgg gccgtatctc aactgaagtt      600

gatgcgcgtc tttcctatga caccgaagcg tcaattgcga aagcaaaacg cctgatcaaa      660

ctctacaacg atgctggtat tagcaacgat cgtattctga tcaaactggc ttctacctgg      720

cagggtatcc gtgctgcaga acagctggaa aaagaaggca tcaactgtaa cctgaccctg      780

ctgttctcct tcgctcaggc tcgtgcttgt gcggaagcgg gcgtgttcct gatctcgccg      840

tttgttggcc gtattcttga ctggtacaaa gcgaataccg ataagaaaga gtacgctccg      900

gcagaagatc cgggcgtggt ttctgtatct gaaatctacc agtactacaa agagcacggt      960

tatgaaaccg tggttatggg cgcaagcttc cgtaacatcg gcgaaattct ggaactggca     1020

ggctgcgacc gtctgaccat cgcaccggca ctgctgaaag agctggcgga gagcgaaggg     1080

gctatcgaac gtaaactgtc ttacaccggc gaagtgaaag cgcgtccggc gcgtatcact     1140

gagtccgagt tcctgtggca gcacaaccag gatccaatgg cagtagataa actggcggaa     1200

ggtatccgta agtttgctat tgaccaggaa aaactggaaa aaatgatcgg cgatctgctg     1260

taatctagac gatctggagt caaaatgtcc tcacgtaaag agcttgccaa tgctattcgt     1320

gcgctgagca tggacgcagt acagaaagcc aaatccggtc acccgggggc ccctatgggt     1380

atggctgaca ttgccgaagt cctgtggcgt gatttcctga aacacaaccc gcagaatccg     1440

tcctgggctg accgtgaccg cttcgtgctg tccaacggcc acggctccat gctgatctac     1500

agcctgctgc acctcaccgg ttacgatctg ccgatggaag aactgaaaaa cttccgtcag     1560

ctgcactcta aaactccggg tcacccggaa gtgggttaca ccgctggtgt ggaaaccacc     1620

accggtccgc tgggtcaggg tattgccaac gcagtcggta tggcgattgc agaaaaaacg     1680

ctggcggcgc agtttaaccg tccgggccac gacattgtcg accactacac ctacgccttc     1740

atgggcgacg gctgcatgat ggaaggcatc tcccacgaag tttgctctct ggcgggtacg     1800

ctgaagctgg gtaaactgat tgcattctac gatgacaacg gtatttctat cgatggtcac     1860

gttgaaggct ggttcaccga cgacaccgca atgcgtttcg aagcttacgg ctggcacgtt     1920

attcgcgaca tcgacggtca tgacgcggca tctatcaaac gcgcagtaga agaagcgcgc     1980

gcagtgactg acaaaccttc cctgctgatg tgcaaaacca tcatcggttt cggttccccg     2040

aacaaagccg gtacccacga ctcccacggt gcgccgctgg gcgacgctga aattgccctg     2100

acccgcgaac aactgggctg gaaatatgcg ccgttcgaaa tcccgtctga aatctatgct     2160

cagtgggatg cgaaagaagc aggccaggcg aaagaatccg catggaacga gaaattcgct     2220

gcttacgcga aagcttatcc gcaggaagcc gctgaattta cccgccgtat gaaaggcgaa     2280

atgccgtctg acttcgacgc taaagcgaaa gagttcatcg ctaaactgca ggctaatccg     2340

gcgaaaatcg ccagccgtaa agcgtctcag aatgctatcg aagcgttcgg tccgctgttg     2400

ccggaattcc tcggcggttc tgctgacctg gcgccgtcta acctgaccct gtggtctggt     2460

tctaaagcaa tcaacgaaga tgctgcgggt aactacatcc actacggtgt tcgcgagttc     2520

ggtatgaccg cgattgctaa cggtatctcc ctgcacggtg gcttcctgcc gtacacctcc     2580

accttcctga tgttcgtgga atacgcacgt aacgccgtac gtatggctgc gctgatgaaa     2640

cagcgtcagg tgatggttta cacccacgac tccatcggtc tgggcgaaga cgggccgact     2700

caccagccgg ttgagcaggt cgcttctctg cgcgtaaccc cgaacatgtc tacatggcgt     2760

ccgtgtgacc aggttgaatc cgcggtcgcg tggaaatacg gtgttgagcg tcaggacggc     2820

ccgaccgcac tgatcctctc ccgtcagaac ctggcgcagc aggaacgaac tgaagagcaa     2880

ctggcaaaca tcgcgcgcgg tggttatgtg ctgaaagact gcgccggtca gccggaactg     2940

attttcatcg ctaccggttc agaagttgaa ctggctgttg ctgcctacga aaaactgact     3000

gccgaaggcg tgaaagcgcg cgtggtgtcc atgtcgtcta ccgacgcatt tgacaagcag     3060

gatgctgctt accgtgaatc cgtactgccg aaagcggtta ctgcacgcgt tgctgtagaa     3120

gcgggtattg ctgactactg gtacaagtat gttggcctga acggtgctat cgtcggtatg     3180

accaccttcg gtgaatctgc tccggcagag ctgctgtttg aagagttcgg cttcactgtt     3240

gataacgttg ttgcgaaagc aaaagaactg ctgtaattag catttcgggt aaaaaaggtc     3300

gcttcggcga ccttttttat taccttgata atgtccgttt gcgcggcgcg ccccagttac     3360

tcaatacgta acaataatca gtttatccta actatagaat cgcatgagaa gcgataacgt     3420

ttcaccataa gcaatatatt cattgcaaca gtggaattgc cttatgcgtc aaggaaggat     3480

agatcattga cggactgagt tcaaaaagag actcgtctaa aagattttaa gaaaggtttc     3540

gatatgacct ctgctgtgcc atcaaatacg aaaaaaaagc tggtgattgc ttccgatcac     3600

gcagcatttg agttgaaatc aaccttgatt acttggctga aagagcttgg tcatgaggtc     3660

gaagaccttg gccctcatga aaaccattca gtcgattatc ccgattacgg ttataagctg     3720

gctgtcgcta tcgcagaaaa aaccgctgat ttcggtattg ctttatgtgg ctcgggaatc     3780

ggtatctcga tcgctgtcaa tcgccatccg gctgcccgtt gcgctttgat tacggataac     3840

cttaccgccc gtttggcaag agaacataac aatgccaatg ttatcgctat gggtgcgaga     3900

ttgatcggca ttgaaaccgc taaggattgt atttcagctt tccttgcaac gccgtttgga     3960

ggtgaacgtc atgttcgccg tatcgataaa ctttcgaatc ctcagttcaa tatctagata     4020

agttaggaga ataaacatga gtaaattacc cctgattgct ccctctatcc tttcggcgga     4080

ttttgcccat ttgggagatg aggtcgcggc gatagatcag gccggtgccg attggatcca     4140

tattgatgtg atggatggcc atttcgtgcc gaatatcacc ataggcccca tggttgtgaa     4200

ggctttgcgt ccctatagcc aaaagccttt tgatgtccat ttgatgattg cgcctgtcga     4260

tcaatatatc gaggcttttt ctgaagcggg tgctgatatt atcagtttcc atcccgaagc     4320

gggcgcgcat ccccatcgca ctattcagca tatcaaatca ttgggcaaaa aagcgggatt     4380

agtttttaat ccggcgaccc ctttaagctg gcttgattat ctaatggatg atcttgatct     4440

gattatggtg atgagcgtta accccggttt tggcggccaa aaatttatca aaacccaatt     4500

agaaaagatt aaagatatcc gtcaaagaat taccgcctct gggcgggata tccgcttgga     4560

agtggatggc ggaattgatg ccacgactgc accgcttgcc gtcgaagccg gtgccgatgt     4620

tttggtcgcg ggaacggcca gctttaaagg cggcgcaaca tgttacaccg ataatatcag     4680

gatattgcgt aaatcatgat taattaactc gaggcggcct gaacgtactg caagtcctga     4740

cgtcactgtg cagtccgttg gcccggttat cggtagcgat accgggcatt tttttaagga     4800

acgatcgata gaattcgcgg ccggcccggc aagacgtgat atggaaccgg aatttgctcc     4860

ggcattcctg cgcaaagata gctaatatct ttcatatttt gtatcgaaaa aggagggtct     4920

ttaaagatcc tccttttttt tgcataaaaa gaaggccata gaacaaacag tgataaagac     4980

agtctcaaac tgtcttttta tagaaaatac cagaatattg tatctggggg aggatgcatg     5040

gtcttaatcc ggaatacccc ggtcatgcac aggatgttag agcttttgcc tttatggcaa     5100

aataaaccat ggctcgggaa tatctgcgct ttgatttttg taggatgtgc cttccttgtc     5160

cgtagtatta ttgggcattt tttaccggca ggttatcctt tcgtgacctt tatgccgaca     5220

atgcttgtgg ttactttcct ctttgggaca agaccgggta ttatcgcggc tattcttagc     5280

ttgatggttg cgccttattt tatcgaagaa ggaagccgat ttaacggtgt attggtctgg     5340

tttctttgcc tgctagaaac agtcactgat atgggattgg tgattgcgct acagcaaggt     5400

aattaccgcc tccagaaaaa gcgtgcctat aatcagatgc tggctgaacg caatgagttg     5460

ctgtttcatg aattacagca tcgcatttca aataacttac aggttattgc gtcattattg     5520

cggatgcaaa gccgcagcat caccgatgaa aaagccaagg aagctattga tgcctctgtt     5580

cgtcggattc atatgatcgg tgaattacag cgggcgcttt atattaaaaa cgggaatcag     5640

cttggggcaa aattgatcct tgatcgcttg atcaaagagg tcattgcgtc cagtaatctc     5700

ccgaacatcc gctataaaat agaagctgaa gacctgatct taccgtcaga tatggcaatc     5760

cctttagcgc ttgtatctgc tgaatccgtt tcaaacgcgt tagagcatgg ctttaaaggc     5820

gatcataaag acgcgtttat tgaaattaag cttcaaaaaa ttagcgggca aatcgaactt     5880

accatttcca ataatggcaa acctcttccc caaggctttt cccttgaaaa ggtcgatagc     5940

ttaggcctga aaattgcggc tatgtttgcc cgacaattca aaggaaaatt caccttaagt     6000

aatcagccta accgttatgt ggtttctagc cttattttgc cttgcggtta ggcggccgcc     6060

taattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt     6120

gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt     6180

ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga     6240

tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga     6300

aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt     6360

ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc     6420

ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat     6480

ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt     6540

gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg     6600

acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact     6660

ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa     6720

aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc     6780

actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc     6840

ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa     6900

agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc     6960

agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc     7020

tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc     7080

gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac     7140

tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt     7200

gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt     7260

agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg     7320

tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt     7380

cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc     7440

aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca     7500

atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc     7560

atgtttgaca gcttatcatc gatgtgacgg aagatcactt cgcagaataa ataaatcctg     7620

gtgtccctgt tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc     7680

ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt     7740

ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat     7800

ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag     7860

ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg     7920

taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga     7980

atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg     8040

ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg     8100

aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg     8160

gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca     8220

atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg     8280

cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg     8340

cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat     8400

tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt     8460

gcccttaaac gcctggttgc tacgcctgaa taagtcgacc tttgtagtct tggcctgttg     8520

tgtgcatgag caaatcaatg gcaccacccc ctcctttttg agctgaatgg tcataaaatt     8580

tataattatc tatcgtaatt cggaatctat gttcagggtc tcgccattgc tttttgtctg     8640

ctgggtcaag ttccatgcct aaggttttta agacatcaga aagaggtatt gcacgcatgc     8700

tatcagcttt tcttctagct aatgacaggg cttcctctgc tctatctgct cgtttttttt     8760

cttccacata tctcgccgct ttgtcagcca gcggctgtat tacggaaagt gccgattttt     8820

gggcttttag gcgttctttt tctgcccatt cttccttatt tgtaaaaatt gagggtggga     8880

tgggtgcctg aatcttggga tctagctgta aagttttgtt gatatttccg taatgtcttt     8940

ggactctttg atgcgttgct tttgaacctt ttacgcctct ggccagccct agaggctcca     9000

tagaagccgc ataatccgtc tggagggcag aaagggcttt tcgaccatca aaccatctcg     9060

atgcgtttaa acggcctgta tcggggtctc taggcaccat aaagccggtt aagtggggtg     9120

ttgtttcatc agcatgtagc tgaagagata caaggttgtt ttctccaaag gtttgttccg     9180

cccattgctg ggtgattgtt ttccagtgtt cgagtttttc aggagtggcc tgttttgacc     9240

attctggaga cataccaaag aacagttcta tggcctgcac accgtttttt ctaagaggct     9300

ttcccgtttc tttctgaatt ttattcagca tagatttaac atctgctgat gggtcagtag     9360

agcctttgag tatttcgttt agttcttttc tatctgggtc agcgttttgt gtttcgcggc     9420

ctcgcgtcat atgcaggctc gcggctttaa tcgtgccaac tgttttatgt ttttcaaacc     9480

taaagattgc atagttcggc atgttttaac tgctttaatt tgagaaaaga ccagaggaaa     9540

taatccagcc tatatttctt tccctagtag cgaactggaa ttgtttttcc gaaggaaaaa     9600

agcaattccg tagtgagtac tgaatttatt ctgattcgtc ttgcttttgg agcgtctttt     9660

tgcgttctat aactgttgtg aaagctacgc ggtcgccatt gaaaacgaaa ttaggattaa     9720

taaaatacca tccttggcga acatgctttg caatgatttt agctttttct aattcggcta     9780

gacctcttgc aaaggtagct tgagatagtg ccagtttttt ttcttgtgcg ttaagaaagt     9840

cctctaaaac gaatttgtct aaagggacga ggtctttgct gatgcctttg tcttgaagta     9900

tccaaaccag aacgctgaaa gcttttattc cagcggctcc tagttcaaaa gttagcgcga     9960

tattggtgct aaataatttt acaaattctt cactatcaac acgtctgtaa gtcgtcacat    10020

gagtgccttg catctcacca gtggcttgat tgaccagaat gttatcatct cgtcctaatc    10080

gagataactg aaccctctga cttttaactg gcacaaccat accttcgatg aaaggattct    10140

cgtcatatct gattggctgc tttctcaatt ttgtcgccat atttgataaa cctttaatca    10200

aaaaaaccac attttttgat tatacctatt catcgaatga ggcaaggtct atcaatttta    10260

cccctttttt tgatagacgg tttaatcaat attgatagac cccttcacag attctgaaaa    10320

tcgacttccc tattttaggg atattttcac gattcccttt cttagttctt cctagtgggg    10380

aaattcgttg aatcctgcct cggaaaaacc atgagaaagc tgttggttat atacacgggc    10440

aaagccaccc tatttttagc tactggggaa agagataagg cagggtattt gtaaaattaa    10500

aaccggattt ttcgctttac ggtttgttta ggcgcaactg tctttttaag accgcgttta    10560

accatcaaaa gatcgttcca atcttttccg tgtatcatct gttctttagg tgggagccag    10620

ttttcaactt tttttgttgg aaacgcggct ttaatcgctc cgactaatag cgatgctgct    10680

ctttgtccta cagcatccca atcataggca atatggacag aagatgcctt ttcaacgatt    10740

tttcggagag ttttagtaag agacgttctt acgccgctgg tgcttaataa ttttacgcca    10800

gctttaattt tttctgggct taaaaagccg actactgaaa tcgcgtctat cgcactttca    10860

gcgatataaa gatcatactt ttcgtcattt tttacattga tgctgccagt aaaatgggct    10920

tcgcgactgc ttcccaaggc taacccttta aaaccactgc ttgttccgcg taattctgcg    10980

ccctgaagtg tatctttatc gtcatacatc aagaaggcta cattaccgcg atcatctgtt    11040

cggatagagt caggaatatt gttaaatgat attcctcggg cagcgttggg tcctggccac    11100

gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta    11160

ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa    11220

cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg    11280

aaacgcggaa gtcccctacg tgctgctgaa gttgcccgca acagagagtg gaaccaaccg    11340

gtgataccac gatactatga ctgagagtca acgccatggg agctccctat cgtctgactc    11400

gcaaggctga acgtgttgac gccttgagca aggccaaagc ggttcttgac gaagccttcc    11460

cagaagctga tccgacagaa aagctgcgca tccagaagct tgcgaagaag ctggaagcaa    11520

aaatcgtccg caccgccatt ctgaaagaag gccggagaat tgacggacgc gatctgaaaa    11580

cagttcgccc gatccgctct caggttggat tcttgccccg cacgcatggt tctgccctgt    11640

ttacgcgtgg tgaaacacag gctttggttt caaccaccct tggaacggcg gatgctgaac    11700

agatgatcga cggtttaacc ggccttcatt atgaacgctt catgctgcat tacaacttcc    11760

ccccatattc ggtcggtgaa gttggtcgtt ttggtgctcc gggtcgtcgt gaaatcggcc    11820

atggtaaact ggcatggcgt gcgcttcatc cggttttgcc gagcaaggct gatttcccgt    11880

ataccatccg tgttttgtcg gatatcaccg aatctaatgg ttcctcttcc atggcaaccg    11940

tttgcggtgg ctgccttgca ttgatggatg ccggtgttcc cttaacgcgt ccggtttccg    12000

gtatcgccat gggtcttatt ctggaaaaag acggcttcgc tattttgtcc gatatcatgg    12060

gtgatgaaga tcacttgggt gatatggact ttaaggtcgc cggtaccgaa aaaggtatca    12120

ccagcctcca gatggacatc aaggttgctg gcattaccga agaaatcatg cagaaagctt    12180

tggaacaggc taaaggtggc cgtgctcata tcttgggtga aatgtccaaa gcgctgggtg    12240

aagtccgctc cgaaatttct aatttggcac cgcgcattga aacaatgagc gtaccaaaag    12300

acaaaatccg tgatgttatc ggaacgggcg gaaaagttat ccgtgaaatc gtggcgacca    12360

caggtgccaa ggtcgatatc gaagatgacg gcacggttcg tctgtcttct tctgatccgg    12420

ccaatattga agcagcccgt gaatggatca atggtattgt tgaagaaccg gaagtaggca    12480

aaatctataa cggtaaagtc gtcaatatcg ttgatttcgg tgccttcgta aacttcatgg    12540

gtggccgtga cggcttggta catgtttcgg aaatcaagaa cgaacgtgtc aacaaggtca    12600

gcgatgtcct gtccgaaggt caggaagtca aagtcaaggt tcttgaaatt gacaaccgtg    12660

gcaaggttcg cctgtctatg cgtgttgtcg atcaggaaac cgga                     12704


<210>  30
<211>  1318
<212>  DNA
<213>  artificial sequence

<220>
<223>  constructed fragment for integration into Z. m pnp gene

<400>  30
cctatcgtct gactcgcaag gctgaacgtg ttgacgcctt gagcaaggcc aaagcggttc       60

ttgacgaagc cttcccagaa gctgatccga cagaaaagct gcgcatccag aagcttgcga      120

agaagctgga agcaaaaatc gtccgcaccg ccattctgaa agaaggccgg agaattgacg      180

gacgcgatct gaaaacagtt cgcccgatcc gctctcaggt tggattcttg ccccgcacgc      240

atggttctgc cctgtttacg cgtggtgaaa cacaggcttt ggtttcaacc acccttggaa      300

cggcggatgc tgaacagatg atcgacggtt taaccggcct tcattatgaa cgcttcatgc      360

tgcattacaa cttcccccca tattcggtcg gtgaagttgg tcgttttggt gctccgggtc      420

gtcgtgaaat cggccatggt aaactggcat ggcgtgcgct tcatccggtt ttgccgagca      480

aggctgattt cccgtatacc atccgtgttt tgtcggatat caccgaatct aatggttcct      540

cttccatggc aaccgtttgc ggtggctgcc ttgcattgat ggatgccggt gttcccttaa      600

cgcgtccggt ttccggtatc gccatgggtc ttattctgga aaaagacggc ttcgctattt      660

tgtccgatat catgggtgat gaagatcact tgggtgatat ggactttaag gtcgccggta      720

ccgaaaaagg tatcaccagc ctccagatgg acatcaaggt tgctggcatt accgaagaaa      780

tcatgcagaa agctttggaa caggctaaag gtggccgtgc tcatatcttg ggtgaaatgt      840

ccaaagcgct gggtgaagtc cgctccgaaa tttctaattt ggcaccgcgc attgaaacaa      900

tgagcgtacc aaaagacaaa atccgtgatg ttatcggaac gggcggaaaa gttatccgtg      960

aaatcgtggc gaccacaggt gccaaggtcg atatcgaaga tgacggcacg gttcgtctgt     1020

cttcttctga tccggccaat attgaagcag cccgtgaatg gatcaatggt attgttgaag     1080

aaccggaagt aggcaaaatc tataacggta aagtcgtcaa tatcgttgat ttcggtgcct     1140

tcgtaaactt catgggtggc cgtgacggct tggtacatgt ttcggaaatc aagaacgaac     1200

gtgtcaacaa ggtcagcgat gtcctgtccg aaggtcagga agtcaaagtc aaggttcttg     1260

aaattgacaa ccgtggcaag gttcgcctgt ctatgcgtgt tgtcgatcag gaaaccgg       1318


<210>  31
<211>  1225
<212>  DNA
<213>  artificial sequence

<220>
<223>  constructed fragment for integration into the Z. m pnp gene

<400>  31
cggcaagacg tgatatggaa ccggaatttg ctccggcatt cctgcgcaaa gatagctaat       60

atctttcata ttttgtatcg aaaaaggagg gtctttaaag atcctccttt tttttgcata      120

aaaagaaggc catagaacaa acagtgataa agacagtctc aaactgtctt tttatagaaa      180

ataccagaat attgtatctg ggggaggatg catggtctta atccggaata ccccggtcat      240

gcacaggatg ttagagcttt tgcctttatg gcaaaataaa ccatggctcg ggaatatctg      300

cgctttgatt tttgtaggat gtgccttcct tgtccgtagt attattgggc attttttacc      360

ggcaggttat cctttcgtga cctttatgcc gacaatgctt gtggttactt tcctctttgg      420

gacaagaccg ggtattatcg cggctattct tagcttgatg gttgcgcctt attttatcga      480

agaaggaagc cgatttaacg gtgtattggt ctggtttctt tgcctgctag aaacagtcac      540

tgatatggga ttggtgattg cgctacagca aggtaattac cgcctccaga aaaagcgtgc      600

ctataatcag atgctggctg aacgcaatga gttgctgttt catgaattac agcatcgcat      660

ttcaaataac ttacaggtta ttgcgtcatt attgcggatg caaagccgca gcatcaccga      720

tgaaaaagcc aaggaagcta ttgatgcctc tgttcgtcgg attcatatga tcggtgaatt      780

acagcgggcg ctttatatta aaaacgggaa tcagcttggg gcaaaattga tccttgatcg      840

cttgatcaaa gaggtcattg cgtccagtaa tctcccgaac atccgctata aaatagaagc      900

tgaagacctg atcttaccgt cagatatggc aatcccttta gcgcttgtat ctgctgaatc      960

cgtttcaaac gcgttagagc atggctttaa aggcgatcat aaagacgcgt ttattgaaat     1020

taagcttcaa aaaattagcg ggcaaatcga acttaccatt tccaataatg gcaaacctct     1080

tccccaaggc ttttcccttg aaaaggtcga tagcttaggc ctgaaaattg cggctatgtt     1140

tgcccgacaa ttcaaaggaa aattcacctt aagtaatcag cctaaccgtt atgtggtttc     1200

tagccttatt ttgccttgcg gttag                                           1225


<210>  32
<211>  25
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  32
cggcttcaat cggattgtta gcagg                                             25


<210>  33
<211>  38
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  33
cgtgtagctt ggacactcat gtttattctc ctaactta                               38


<210>  34
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  34
ccagtatcag cccgtcatac                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  35
ccagcatggt tgtgatggct                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  36
gccttgggct tttaaagcct                                                   20


<210>  37
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  37
gagaagggtt ggttgtggca tc                                                22


<210>  38
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  38
gtggatggcg gaattgatgc ca                                                22


<210>  39
<211>  26
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  39
tctcggagag atagaggtca gtcgac                                            26


<210>  40
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  40
cagctatgat gacagcgcat tgg                                               23


<210>  41
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic primer

<400>  41
gggcggttcg atccatagaa agg                                               23


<210>  42
<211>  2247
<212>  DNA
<213>  Zymomonas mobilis

<400>  42
atgttcgata ttaaacgcca ggaaatcgat tggggcggaa aaaaactgac actggaaacc       60

ggacaggttg cccgtcaggc agatggcgcc gtcattgcga ccttaggtga aacggtcgta      120

ttatgcgcgg taacggcagc aaaaacggta aaagaaggtc aggatttctt tcctttgacc      180

gtccattatc aggaaaaata ttcagcagca ggccgtattc ccggtggctt tttcaagcgt      240

gaacgtggcg caaccgaacg ggaaacgctg atttcacggt taatcgaccg tccaatccgt      300

cctctgtttc cggaaggttt ctataacgaa accttggtca ttgcgcaggt catgtcctat      360

gacggcgaga atgaaccgga tatcttggcg atgatcgctg cttctgcggc tcttgctctt      420

tccggtgtgc ctttcttggg ccccatcggt gctgcccgtg tgggttatca agatggcgag      480

ttcattctta acccgacctt ggaacagctt gaaaaaagtg atcttgatct ggttgtcggg      540

gctacccgtg atgccgtgat gatggttgaa tcggaagcga atgagcttcc cgaagaagtc      600

atgctcaatg ccgtttcttt tgcgcatgaa tctttacagc cggttatcaa agctatcatc      660

aatctggcag aacaggccgc taaagagcct tgggaactgg tcagctatga tgacagcgca      720

ttggctgcca aagtcgaaga actctgctac gacaatttcg ataaggccta tcgtctgact      780

cgcaaggctg aacgtgttga cgccttgagc aaggccaaag cggttcttga cgaagccttc      840

ccagaagctg atccgacaga aaagctgcgc atccagaagc ttgcgaagaa gctggaagca      900

aaaatcgtcc gcaccgccat tctgaaagaa ggccggagaa ttgacggacg cgatctgaaa      960

acagttcgcc cgatccgctc tcaggttgga ttcttgcccc gcacgcatgg ttctgccctg     1020

tttacgcgtg gtgaaacaca ggctttggtt tcaaccaccc ttggaacggc ggatgctgaa     1080

cagatgatcg acggtttaac cggccttcat tatgaacgct tcatgctgca ttacaacttc     1140

cccccatatt cggtcggtga agttggtcgt tttggtgctc cgggtcgtcg tgaaatcggc     1200

catggtaaac tggcatggcg tgcgcttcat ccggttttgc cgagcaaggc tgatttcccg     1260

tataccatcc gtgttttgtc ggatatcacc gaatctaatg gttcctcttc catggcaacc     1320

gtttgcggtg gctgccttgc attgatggat gccggtgttc ccttaacgcg tccggtttcc     1380

ggtatcgcca tgggtcttat tctggaaaaa gacggcttcg ctattttgtc cgatatcatg     1440

ggtgatgaag atcacttggg tgatatggac tttaaggtcg ccggtaccga aaaaggtatc     1500

accagcctcc agatggacat caaggttgct ggcattaccg aagaaatcat gcagaaagct     1560

ttggaacagg ctaaaggtgg ccgtgctcat atcttgggtg aaatgtccaa agcgctgggt     1620

gaagtccgct ccgaaatttc taatttggca ccgcgcattg aaacaatgag cgtaccaaaa     1680

gacaaaatcc gtgatgttat cggaacgggc ggaaaagtta tccgtgaaat cgtggcgacc     1740

acaggtgcca aggtcgatat cgaagatgac ggcacggttc gtctgtcttc ttctgatccg     1800

gccaatattg aagcagcccg tgaatggatc aatggtattg ttgaagaacc ggaagtaggc     1860

aaaatctata acggtaaagt cgtcaatatc gttgatttcg gtgccttcgt aaacttcatg     1920

ggtggccgtg acggcttggt acatgtttcg gaaatcaaga acgaacgtgt caacaaggtc     1980

agcgatgtcc tgtccgaagg tcaggaagtc aaagtcaagg ttcttgaaat tgacaaccgt     2040

ggcaaggttc gcctgtctat gcgtgttgtc gatcaggaaa ccggcgcaga gctggatgat     2100

aaccgtccgc cacgtgagaa cgcagaacgt cgcggtggtg agcgtcctcg tcgtgatcgg     2160

ggccctcgtc gggaatctgg cgatcgtccg gcaagacgtg atatggaacc ggaatttgct     2220

ccggcattcc tgcgcaaaga tagctaa                                         2247


<210>  43
<211>  474
<212>  DNA
<213>  artificial sequence

<220>
<223>  start codon changed to ATG

<400>  43
atgacctctg ctgtgccatc aaatacgaaa aaaaagctgg tgattgcttc cgatcacgca       60

gcatttgagt tgaaatcaac cttgattact tggctgaaag agcttggtca tgaggtcgaa      120

gaccttggcc ctcatgaaaa ccattcagtc gattatcccg attacggtta taagctggct      180

gtcgctatcg cagaaaaaac cgctgatttc ggtattgctt tatgtggctc gggaatcggt      240

atctcgatcg ctgtcaatcg ccatccggct gcccgttgcg ctttgattac ggataacctt      300

accgcccgtt tggcaagaga acataacaat gccaatgtta tcgctatggg tgcgagattg      360

atcggcattg aaaccgctaa ggattgtatt tcagctttcc ttgcaacgcc gtttggaggt      420

gaacgtcatg ttcgccgtat cgataaactt tcgaatcctc agttcaatat ctag            474


<210>  44
<211>  39
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  44
catcttactg cggccgcgtg acggaagatc acttcgcag                              39


<210>  45
<211>  37
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  45
tcactcattt aattaactta ttcaggcgta gcaccag                                37


<210>  46
<211>  1002
<212>  DNA
<213>  artificial sequence

<220>
<223>  constructed fragment that contains a Cmr-cassette that is flanked
       by two wild type loxP sites

<400>  46
ccataacttc gtatagcata cattatacga agttatttaa ttaacttatt caggcgtagc       60

aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact      120

catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg      180

catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc      240

ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg      300

tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga      360

aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc      420

ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa      480

cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac      540

ggaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact      600

tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt      660

tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg      720

atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg      780

aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt      840

tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc      900

cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacgcggc      960

cgcataactt cgtatagcat acattatacg aagttatgcg at                        1002


<210>  47
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  47
ctactcatcc tgcaggcttc tcggtgatcg tgttgc                                 36


<210>  48
<211>  38
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  48
tcactcatgg ccggccgaac agatcgacgg tattgatg                               38


<210>  49
<211>  37
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  49
catcttactg cgatcgcgat caatcgcccg atgaatg                                37


<210>  50
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  50
catcttactg gcgcgcctcg ccgtattgta tcgctg                                 36


<210>  51
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  51
ctacttcact tcatgaccgg                                                   20


<210>  52
<211>  35
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  52
agtcatgcag gcctctgatg aatgctcatc cggaa                                  35


<210>  53
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  53
gtctgacgtt gatcctgatc                                                   20


<210>  54
<211>  38
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  54
tcactcatgg ccggcctgcg tataatattt gcccatgg                               38


<210>  55
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  55
gttcctgctt tgcttttgtg g                                                 21


<210>  56
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  56
cccggaagct atcaaaattt tg                                                22


<210>  57
<211>  18
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  57
caccgtagtg gccgacac                                                     18


<210>  58
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  58
gatggttttg cacatcagca g                                                 21


<210>  59
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  59
tctgcaccga taggattggg                                                   20


<210>  60
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  60
gatgtctttg tctatttcgc g                                                 21


<210>  61
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  61
ccaacgactt cttcagcatg                                                   20


<210>  62
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  62
cccaatccta tcggtgcaga                                                   20


<210>  63
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  63
cgcgaaatag acaaagacat c                                                 21


<210>  64
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  64
catgctgaag aagtcgttgg                                                   20


<210>  65
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  65
ccaaacaagc ttgcatagtt gcc                                               23


<210>  66
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  66
gcagatggtg gttgagcata                                                   20


<210>  67
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  67
tgccattttg tggaacgacg                                                   20


<210>  68
<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  68
tgcagttggt gtgggaatg                                                    19


<210>  69
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  69
ggtttgactc atcaacatgg c                                                 21


<210>  70
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  70
cgtcgttcca caaaatggca                                                   20


<210>  71
<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  71
cattcccaca ccaactgca                                                    19


<210>  72
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  72
gccatgttga tgagtcaaac c                                                 21


