                         SEQUENCE LISTING

<110>  INNATE PHARMA
       PAUL SCHERRER INSTITUT
 
<120>  ENZYMATIC CONJUGATION OF POLYPEPTIDES

<130>  TGase1

<150>  US 61/671,122
<151>  2012-07-13

<150>  US 61/661,569
<151>  2012-06-19

<150>  US 61/579,908
<151>  2011-12-23

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  450
<212>  PRT
<213>  Artificial

<220>
<223>  Murine VH fused to human CH

<400>  1

Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Gly Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Arg Pro Gly His Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Asn Pro Ser Asn Gly Arg Thr Asn Tyr Asn Glu Arg Phe 
    50                  55                  60                  


Lys Ser Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Thr Thr Ala Phe 
65                  70                  75                  80  


Met Gln Leu Ser Gly Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Asp Tyr Tyr Gly Thr Ser Tyr Asn Phe Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Thr Leu Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val 
        115                 120                 125             


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
    130                 135                 140                 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
145                 150                 155                 160 


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
                165                 170                 175     


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
            180                 185                 190         


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
        195                 200                 205             


Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp 
    210                 215                 220                 


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
225                 230                 235                 240 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
                245                 250                 255     


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
            260                 265                 270         


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
        275                 280                 285             


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
    290                 295                 300                 


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
305                 310                 315                 320 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
                325                 330                 335     


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
            340                 345                 350         


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
        355                 360                 365             


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
    370                 375                 380                 


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
385                 390                 395                 400 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
                405                 410                 415     


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
            420                 425                 430         


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
        435                 440                 445             


Gly Lys 
    450 


<210>  2
<211>  214
<212>  PRT
<213>  Artificial

<220>
<223>  Murine VK fused to human Ck

<400>  2

Asp Ile Gln Met Thr Gln Ser Ser Ser Ser Phe Ser Val Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Asn Glu Asp Ile Asn Asn Arg 
            20                  25                  30          


Leu Ala Thr Tyr Gln Gln Thr Pro Gly Asn Ser Pro Arg Leu Leu Ile 
        35                  40                  45              


Ser Gly Ala Thr Asn Leu Val Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Lys Asp Tyr Thr Leu Thr Ile Thr Ser Leu Gln Ala 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Trp Ser Thr Pro Phe 
                85                  90                  95      


Thr Phe Gly Ser Gly Thr Glu Leu Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  3
<211>  23
<212>  PRT
<213>  homo sapiens


<220>
<221>  X
<222>  (12)..(12)
<223>  X can be Tyr or Phe

<220>
<221>  X
<222>  (13)..(13)
<223>  X can be any amino acid other than Asp

<400>  3

His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Xaa Xaa Ser Thr Tyr 
1               5                   10                  15      


Arg Val Val Ser Val Leu Thr 
            20              


<210>  4
<211>  23
<212>  PRT
<213>  homo sapiens


<220>
<221>  X
<222>  (12)..(12)
<223>  X can be Tyr or Phe

<220>
<221>  X
<222>  (15)..(15)
<223>  X can be any amino acid other than Thr

<400>  4

His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Xaa Asn Ser Xaa Tyr 
1               5                   10                  15      


Arg Val Val Ser Val Leu Thr 
            20              


<210>  5
<211>  989
<212>  DNA
<213>  homo sapiens

<400>  5
gggcccaagc gtgttccccc tggcccccag cagcaagagc accagcggcg gcacagccgc       60

cctgggctgc ctggtgaagg actacttccc cgagcccgtg accgtgtcct ggaacagcgg      120

agccctgacc tccggcgtgc acaccttccc cgccgtgctg cagagcagcg gcctgtacag      180

cctgagcagc gtggtgaccg tgcccagcag cagcctgggc acccagacct acatctgtaa      240

cgtgaaccac aagcccagca acaccaaggt ggacaagaga gtggagccca agagctgtga      300

caagacccac acctgccccc cctgcccagc ccccgagctg ctgggcggac ccagcgtgtt      360

cctgttcccc cccaagccca aggacaccct gatgatcagc agaacccccg aggtgacctg      420

tgtggtggtg gacgtgtccc acgaggaccc agaggtgaag ttcaactggt acgtggacgg      480

cgtggaggtg cacaacgcca agaccaagcc cagagaggag cagtacagca gcacctacag      540

ggtggtgtcc gtgctgaccg tgctgcacca ggactggctg aacggcaagg agtacaagtg      600

taaggtgtcc aacaaggccc tgccagcccc aatcgaaaag accatcagca aggccaaggg      660

ccagccaaga gagccccagg tgtacaccct gccacccagc agggaggaga tgaccaagaa      720

ccaggtgtcc ctgacctgtc tggtgaaggg cttctaccca agcgacatcg ccgtggagtg      780

ggagagcaac ggccagcccg agaacaacta caagaccacc cccccagtgc tggacagcga      840

cggcagcttc ttcctgtaca gcaagctgac cgtggacaag agcagatggc agcagggcaa      900

cgtgttcagc tgctccgtga tgcacgaggc cctgcacaac cactacaccc agaagagcct      960

gagcctgtcc ccaggcaagt gatgaattc                                        989


<210>  6
<211>  326
<212>  PRT
<213>  homo sapiens

<400>  6

Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
1               5                   10                  15      


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
            20                  25                  30          


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
        35                  40                  45              


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
    50                  55                  60                  


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
65                  70                  75                  80  


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro 
                85                  90                  95      


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
            100                 105                 110         


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
        115                 120                 125             


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
    130                 135                 140                 


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
145                 150                 155                 160 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ser 
                165                 170                 175     


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
            180                 185                 190         


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
        195                 200                 205             


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
    210                 215                 220                 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
225                 230                 235                 240 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
                245                 250                 255     


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
            260                 265                 270         


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
    290                 295                 300                 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
305                 310                 315                 320 


Ser Leu Ser Pro Gly Lys 
                325     


<210>  7
<211>  989
<212>  DNA
<213>  homo sapiens

<400>  7
gggcccaagc gtgttccccc tggcccccag cagcaagagc accagcggcg gcacagccgc       60

cctgggctgc ctggtgaagg actacttccc cgagcccgtg accgtgtcct ggaacagcgg      120

agccctgacc tccggcgtgc acaccttccc cgccgtgctg cagagcagcg gcctgtacag      180

cctgagcagc gtggtgaccg tgcccagcag cagcctgggc acccagacct acatctgtaa      240

cgtgaaccac aagcccagca acaccaaggt ggacaagaga gtggagccca agagctgtga      300

caagacccac acctgccccc cctgcccagc ccccgagctg ctgggcggac ccagcgtgtt      360

cctgttcccc cccaagccca aggacaccct gatgatcagc agaacccccg aggtgacctg      420

tgtggtggtg gacgtgtccc acgaggaccc agaggtgaag ttcaactggt acgtggacgg      480

cgtggaggtg cacaacgcca agaccaagcc cagagaggag cagtaccaaa gcacctacag      540

ggtggtgtcc gtgctgaccg tgctgcacca ggactggctg aacggcaagg agtacaagtg      600

taaggtgtcc aacaaggccc tgccagcccc aatcgaaaag accatcagca aggccaaggg      660

ccagccaaga gagccccagg tgtacaccct gccacccagc agggaggaga tgaccaagaa      720

ccaggtgtcc ctgacctgtc tggtgaaggg cttctaccca agcgacatcg ccgtggagtg      780

ggagagcaac ggccagcccg agaacaacta caagaccacc cccccagtgc tggacagcga      840

cggcagcttc ttcctgtaca gcaagctgac cgtggacaag agcagatggc agcagggcaa      900

cgtgttcagc tgctccgtga tgcacgaggc cctgcacaac cactacaccc agaagagcct      960

gagcctgtcc ccaggcaagt gatgaattc                                        989


<210>  8
<211>  326
<212>  PRT
<213>  homo sapiens

<400>  8

Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
1               5                   10                  15      


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
            20                  25                  30          


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
        35                  40                  45              


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
    50                  55                  60                  


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
65                  70                  75                  80  


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro 
                85                  90                  95      


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
            100                 105                 110         


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
        115                 120                 125             


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
    130                 135                 140                 


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
145                 150                 155                 160 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Gln 
                165                 170                 175     


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
            180                 185                 190         


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
        195                 200                 205             


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
    210                 215                 220                 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
225                 230                 235                 240 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
                245                 250                 255     


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
            260                 265                 270         


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
    290                 295                 300                 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
305                 310                 315                 320 


Ser Leu Ser Pro Gly Lys 
                325     


<210>  9
<211>  1017
<212>  DNA
<213>  homo sapiens

<400>  9
gtttgtaagc ttgctagcac caagggccca tcggtcttcc ccctggcacc ctcctccaag       60

agcacctctg ggggcacagc ggccctgggc tgcctggtca aggactactt ccccgaaccg      120

gtgacggtgt cgtggaactc aggcgccctg accagcggcg tgcacacctt cccggctgtc      180

ctacagtcct caggactcta ctccctcagc agcgtggtga ccgtgccctc cagcagcttg      240

ggcacccaga cctacatctg caacgtgaat cacaagccca gcaacaccaa ggtggacaag      300

aaagttgagc ccaaatcttg tgacaaaact cacacatgcc caccgtgccc agcacctgaa      360

ctcctggggg gaccgtcagt cttcctcttc cccccaaaac ccaaggacac cctcatgatc      420

tcccggaccc ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga ccctgaggtc      480

aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa gccgcgggag      540

gagcagtacc aaagcacgta ccgggtggtc agcgtcctca ccgtcctgca ccaggactgg      600

ctgaatggca aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc ccccatcgag      660

aaaaccatct ccaaagccaa agggcagccc cgagaaccac aggtgtacac cctgccccca      720

tcccgggatg agctgaccaa gaaccaggtc agcctgacct gcctggtcaa aggcttctat      780

cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa ctacaagacc      840

acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaagct caccgtggac      900

aagagcaggt ggcagcaggg gaacgtcttc tcatgctccg tgatgcatga ggctctgcac      960

aaccactaca cgcagaagag cctctccctg tctccgggta aatgaggatc cacacac        1017


<210>  10
<211>  326
<212>  PRT
<213>  homo sapiens

<400>  10

Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
1               5                   10                  15      


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
            20                  25                  30          


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
        35                  40                  45              


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
    50                  55                  60                  


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
65                  70                  75                  80  


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
                85                  90                  95      


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
            100                 105                 110         


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
        115                 120                 125             


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
    130                 135                 140                 


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
145                 150                 155                 160 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Gln 
                165                 170                 175     


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
            180                 185                 190         


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
        195                 200                 205             


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
    210                 215                 220                 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn 
225                 230                 235                 240 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
                245                 250                 255     


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
            260                 265                 270         


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
    290                 295                 300                 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
305                 310                 315                 320 


Ser Leu Ser Pro Gly Lys 
                325     


