

SEQUENCE LISTING


<110> GENOMICA S.A.U.

<120> Method for detection of KRAS mutations

<130> PC926820WO

<160> 55

<170> PatentIn version 3.3


<210> 1
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> 3'sequence of ARMS primer for amplification of KRAS mutation
Gly12Ser, the sequence being target-specific

<400> 1
ctgaatataa acttgtggta gttggcgcta					30

<210> 2
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> 3'sequence of ARMS primer for amplification of KRAS mutation
Gly12Arg, the sequence being target-specific

<400> 2
cgtcaaggca ctcttgccta cgacacg						27

<210> 3
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> 3'sequence of ARMS primer for amplification of KRAS mutation
Gly12Cys, the sequence being target-specific

<400> 3
ctgaatataa acttgtggta gttggagatt					30

<210> 4
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> 3'sequence of ARMS primer for amplification of KRAS mutation
Gly12Asp, the sequence being target-specific

<400> 4
ctgaatataa acttgtggta gttggagccg a					31

<210> 5
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> 3'sequence of ARMS primer for amplification of KRAS mutation
Gly12Ala, the sequence being target-specific

<400> 5
ctgaatataa acttgtggta gttgcagatg c					31

<210> 6
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> 3'sequence of ARMS primer for amplification of KRAS mutation
Gly12Val, the sequence being target-specific

<400> 6
cgtcaaggca ctcttgccta cggcaa						26

<210> 7
<211> 34
<212> DNA
<213> Artificial Sequence
<220>
<223> 3'sequence of ARMS primer for amplification of KRAS mutation
Gly13Asp, the sequence being target-specific

<400> 7
ctgaatataa acttgtggta gttggagctg ggga					34

<210> 8
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> 3'sequence of ARMS primer for amplification of KRAS mutation
Gln61His, the sequence being target-specific

<400> 8
tggtccctca ttgcactgta ctccaca						27

<210> 9
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> 3'sequence of ARMS primer for amplification of KRAS mutation
Gln61Leu, the sequence being target-specific

<400> 9
gatattctcg acacagcagt tct						23

<210> 10
<211> 47
<212> DNA
<213> Artificial Sequence
<220>
<223> ARMS primer for amplification of KRAS mutation Gly12Ser

<400> 10
aaggataatt aattaatctg aatataaact tgtggtagtt ggcgcta			47

<210> 11
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> ARMS primer for amplification of KRAS mutation Gly12Arg

<400> 11
aatattgagg ctgcagccgt caaggcactc ttgcctacga cacg			44

<210> 12
<211> 49
<212> DNA
<213> Artificial Sequence
<220>
<223> ARMS primer for amplification of KRAS mutation Gly12Cys

<400> 12
ggctagctag ccgcggtagc tgaatataaa cttgtggtag ttggagatt			49

<210> 13
<211> 49
<212> DNA
<213> Artificial Sequence
<220>
<223> ARMS primer for amplification of KRAS mutation Gly12Asp

<400> 13
cggtatttgg gcaacctgct gaatataaac ttgtggtagt tggagccga			49

<210> 14
<211> 50
<212> DNA
<213> Artificial Sequence
<220>
<223> ARMS primer for amplification of KRAS mutation Gly12Ala

<400> 14	
gaatccatgt gatgacttgc tgaatataaa cttgtggtag ttgcagatgc			50

<210> 15
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> ARMS primer for amplification of KRAS mutation Gly12Val

<400> 15
tcaggcggcc aggatggagc gtcaaggcac tcttgcctac ggcaa			45

<210> 16
<211> 53
<212> DNA
<213> Artificial Sequence
<220>
<223> ARMS primer for amplification of KRAS mutation Gly13Asp

<400> 16
ccgagacgtt cgacactgcc tgaatataaa cttgtggtag ttggagctgg gga		53

<210> 17
<211> 47
<212> DNA
<213> Artificial Sequence
<220>
<223> ARMS primer for amplification of KRAS mutation Gln61His

<400> 17
ttaggctctg aactcggcgt tggtccctca ttgcactgta ctccaca			47

<210> 18
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> ARMS primer for amplification of KRAS mutation Gln61Leu

<400> 18
ggccacttac cgggatccag atattctcga cacagcagtt ct				42

<210> 19
<211> 22
<212> DNA
<213> Artificial Sequence
<220>
<223> Amplification primer to be used in combination with any of
ARMS primers comprising SEQ ID N 1, 4, 7, or
ARMS primers consisting of SEQ ID N 10, 13, 16

<400> 19
ggtcctgcac cagtaatatg ca						22

<210> 20
<211> 22
<212> DNA
<213> Artificial Sequence
<220>
<223> Amplification primer to be used in combination with any of
ARMS primers comprising SEQ ID N 3, 5, or
ARMS primers consisting of SEQ ID N 12, 14

<400> 20
ggtcctgcac cagtaatatg ca						22

<210> 21
<211> 21
<212> DNA
<213> Artificial Sequence
<220>
<223> Amplification primer to be used in combination with any of
ARMS primers comprising SEQ ID N 2, 6, or
ARMS primers consisting of SEQ ID N 11, 15

<400> 21
ggtggagtat ttgatagtgt a							21

<210> 22
<211> 24
<212> DNA
<213> Artificial Sequence
<220>
<223> Amplification primer to be used in combination with any of
ARMS primer comprising SEQ ID N 8, or
ARMS primer consisting of SEQ ID N 17

<400> 22
gtttctccct tctcaggatt ccta						24

<210> 23
<211> 24
<212> DNA
<213> Artificial Sequence
<220>
<223> Amplification primer to be used in combination with any of
ARMS primer comprising SEQ ID N 9, or
ARMS primer consisting of SEQ ID N 18

<400> 23
gggatattac ctacctcata aaca						24

<210> 24
<211> 17
<212> DNA
<213> Artificial Sequence
<220>
<223> 5 sequence of ARMS primer for amplification of KRAS mutation
Gly12Ser, the sequence being non-target-specific.
This sequence is also present in the detection probe of
KRAS mutation Gly12Ser.

<400> 24
aaggataatt aattaat							17

<210> 25
<211> 17
<212> DNA
<213> Artificial Sequence
<220>
<223> 5 sequence of ARMS primer for amplification of KRAS mutation
Gly12Arg, the sequence being non-target-specific.
This sequence is also present in the detection probe of
KRAS mutation Gly12Arg

<400> 25
aatattgagg ctgcagc							17

<210> 26
<211> 19
<212> DNA
<213> Artificial Sequence
<220>
<223> 5 sequence of ARMS primer for amplification of KRAS mutation
Gly12Cys, the sequence being non-target-specific.
This sequence is also present in the detection probe of
KRAS mutation Gly12Cys

<400> 26
ggctagctag ccgcggtag							19

<210> 27
<211> 18
<212> DNA
<213> Artificial Sequence
<220>
<223> 5 sequence of ARMS primer for amplification of KRAS mutation
Gly12Asp, the sequence being non-target-specific.
This sequence is also present in the detection probe of
KRAS mutation Gly12Asp

<400> 27
cggtatttgg gcaacctg							18

<210> 28
<211> 19
<212> DNA
<213> Artificial Sequence
<220>
<223> 5 sequence of ARMS primer for amplification of KRAS mutation
Gly12Ala, the sequence being non-target-specific.
This sequence is also present in the detection probe of
KRAS mutation Gly12Ala

<400> 28
gaatccatgt gatgacttg							19

<210> 29
<211> 19
<212> DNA
<213> Artificial Sequence
<220>
<223> 5 sequence of ARMS primer for amplification of KRAS mutation
Gly12Val, the sequence being non-target-specific.
This sequence is also present in the detection probe of
KRAS mutation Gly12Val

<400> 29
tcaggcggcc aggatggag							19

<210> 30
<211> 19
<212> DNA
<213> Artificial Sequence
<220>
<223> 5 sequence of ARMS primer for amplification of KRAS mutation
Gly13Asp, the sequence being non-target-specific.
This sequence is also present in the detection probe of
KRAS mutation Gly13Asp

<400> 30
ccgagacgtt cgacactgc							19

<210> 31
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223> 5 sequence of ARMS primer for amplification of KRAS mutation
Gln61His, the sequence being non-target-specific.
This sequence is also present in the detection probe of
KRAS mutation Gln61His

<400> 31
ttaggctctg aactcggcgt							20

<210> 32
<211> 19
<212> DNA
<213> Artificial Sequence
<220>
<223> 5 sequence of ARMS primer for amplification of KRAS mutation
Gln61Leu, the sequence being non-target-specific.
This sequence is also present in the detection probe of
KRAS mutation Gln61Leu

<400> 32
ggccacttac cgggatcca							19

<210> 33
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gly12Ser

<400> 33
gcttgccccg gggaaggata attaattaat					30

<210> 34
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gly12Ser

<400> 34
ccccggggaa ggataattaa ttaat						25

<210> 35
<211> 35
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gly12Arg

<400> 35
ttcgccgggt tacccgggaa tattgaggct gcagc					35

<210> 36
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gly12Arg

<400> 36
cgggttaccc gggaatattg aggctgcagc					30

<210> 37
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gly12Cys

<400> 37
ggctagctag ccgcggtagc tgaat						25

<210> 38
<211> 38
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gly12Cys

<400> 38
cgaggccttg gccggctagc tagccgcggt agctgaat				38

<210> 39
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gly12Cys

<400> 39		
cgaggccttg gccggctagc tagccgcggt ag					32

<210> 40
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gly12Cys

<400> 40
ccttggccgg ctagctagcc gcggtagctg aat					33

<210> 41
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gly12Cys

<400> 41
ccttggccgg ctagctagcc gcggtag						27

<210> 42
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gly12Asp

<400> 42
gcttgccccg gggcggtatt tgggcaacct g					31

<210> 43
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gly12Asp

<400> 43
ccccggggcg gtatttgggc aacctg						26

<210> 44
<211> 19
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gly12Ala

<400> 44
gaatccatgt gatgacttg							19

<210> 45
<211> 24
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gly12Ala

<400> 45
gaatccatgt gatgacttga cttg						24

<210> 46
<211> 38
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gly12Ala

<400> 46
cgggttaccc gggagtctcg aatccatgtg atgacttg				38

<210> 47
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gly12Ala

<400> 47
cgggttaccc ggggaatcca tgtgatgact tg					32

<210> 48
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gly12Val

<400> 48
tcaggcggcc aggatggagc tgaat						25

<210> 49
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gly12Val

<400> 49	
cgggttaccc gggtcaggcg gccaggatgg ag					32

<210> 50
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gly13Asp

<400> 50
ccgagacgtt cgacactgcc tgaat						25

<210> 51
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gln61His

<400> 51	
cgggttaccc gggttaggct ctgaactcgg cgt					33

<210> 52
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gln61His

<400> 52
cgggttaccc gggagtctct taggctctga actcggcgt				39

<210> 53
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gln61Leu

<400> 53	
cgggttaccc gggggccact taccgggatc ca					32

<210> 54
<211> 38
<212> DNA
<213> Artificial Sequence
<220>
<223> Probe for detection of ARMS amplification product of 
KRAS mutation Gln61Leu

<400> 54
cgggttaccc gggagtctcg gccacttacc gggatcca				38

<210>  55
<211>  241
<212>  DNA
<213>  Artificial
<220>
<223>  DNA fragment that contains the positions that give rise to the 7 
       KRAS mutations Gly12Ser, Gly12Arg, Gly12Cys, Gly12Asp, Gly12Ala, 
       Gly12Val and Gly13Asp

<400>  55
ggtggagtat ttgatagtgt attaacctta tgtgtgacat gttctaatat agtcacattt       60

tcattatttt tattataagg cctgctgaaa atgactgaat ataaacttgt ggtagttgga      120

gctggtggcg taggcaagag tgccttgacg atacagctaa ttcagaatca ttttgtggac      180

gaatatgatc caacaataga ggtaaatctt gttttaatat gcatattact ggtgcaggac      240

c                                                                      241


