                         SEQUENCE LISTING

<110>  UNIVERSITEIT GENT
 
<120>  A lactonase derived from Candida bombicola and uses thereof

<130>  P2011/098/PCT

<150>  EP11194538.2
<151>  2011-12-20

<160>  48    

<170>  PatentIn version 3.3

<210>  1
<211>  1233
<212>  DNA
<213>  Candida bombicola

<400>  1
atgctggctc tgtttttttc gcttgcgcct ctactttctc aagctctccc tttaggctat     60

actgcggccc ccgctgaatc attctatttt tggccagaga acatatccag cctccaagct    120

ggcgagattt ttagaaaacg ggaactctta actctcccag acatctttga ctttggccct    180

aatctggaaa aggtcgtaca agtggcttac aaaacccgtc tcaccgatgg caatgactcg    240

ttttccatcg ccagtatctt tatccctaag aatccaagcc cagaactcaa actttactct    300

tatcagacgt ttgaggatgc cgtgcagctt gattgtgccc caagctatgc tttagaagtg    360

ggtaacaagt ccagcaacta tcttcctgtc actagcaatt tatctgccat cagtcgagaa    420

cttgagaaag gacgtcactg cattatccct gatcacgagg gctatatttc aggattcttt    480

gcaggacggc aggagggata tgctggttta gacggaattc gcgctgctcg aaactatctc    540

aatggcacca acgagacccc aattggtatc ttcggataca gtggaggtgc acaagcaacg    600

gcctggattg ttgatttgca tgacgagtat gctcctgact tgaactttgt tggaacagtt    660

tctggaggca ctttggttga cgcttggggc acttttcagt atatcgacta tccgaaggtg    720

tatctaaagg gcagcattct tatcatgtat acgggtcttt tttcaggtta tccagctcaa    780

tttgaggtga tttggccata tattgagcct gtaattcaag aaaacatgct actgctacgt    840

ttggcgccga atgattgtaa ccaaagcccg atacttcaag gttacaacaa ttcaatcatg    900

gccggtatac atgtggacct tcccgaattc cctgcttcta agtacatatt ccagcacgag    960

tccctccttg ccaactacag cgtagtgcca gtttccacac cgaagtttcc tcgctacatg   1020

taccatggtg gatctgatga gttggccaaa ttgagccttg tcgagcagta tgttgatcaa   1080

caatggaata ccggcgctaa tctcaccttc gtggtgtatc cgggtcttct tcatgacgag   1140

acggcttacc gtggctttga tgccgcgatg gattggcttg atgcccagct cgatagtgga   1200

taccttccac ctgtaaactc aactcataca tga                                1233


<210>  2
<211>  410
<212>  PRT
<213>  Candida bombicola

<400>  2

Met Leu Ala Leu Phe Phe Ser Leu Ala Pro Leu Leu Ser Gln Ala Leu 
1               5                   10                  15      


Pro Leu Gly Tyr Thr Ala Ala Pro Ala Glu Ser Phe Tyr Phe Trp Pro 
            20                  25                  30          


Glu Asn Ile Ser Ser Leu Gln Ala Gly Glu Ile Phe Arg Lys Arg Glu 
        35                  40                  45              


Leu Leu Thr Leu Pro Asp Ile Phe Asp Phe Gly Pro Asn Leu Glu Lys 
    50                  55                  60                  


Val Val Gln Val Ala Tyr Lys Thr Arg Leu Thr Asp Gly Asn Asp Ser 
65                  70                  75                  80  


Phe Ser Ile Ala Ser Ile Phe Ile Pro Lys Asn Pro Ser Pro Glu Leu 
                85                  90                  95      


Lys Leu Tyr Ser Tyr Gln Thr Phe Glu Asp Ala Val Gln Leu Asp Cys 
            100                 105                 110         


Ala Pro Ser Tyr Ala Leu Glu Val Gly Asn Lys Ser Ser Asn Tyr Leu 
        115                 120                 125             


Pro Val Thr Ser Asn Leu Ser Ala Ile Ser Arg Glu Leu Glu Lys Gly 
    130                 135                 140                 


Arg His Cys Ile Ile Pro Asp His Glu Gly Tyr Ile Ser Gly Phe Phe 
145                 150                 155                 160 


Ala Gly Arg Gln Glu Gly Tyr Ala Gly Leu Asp Gly Ile Arg Ala Ala 
                165                 170                 175     


Arg Asn Tyr Leu Asn Gly Thr Asn Glu Thr Pro Ile Gly Ile Phe Gly 
            180                 185                 190         


Tyr Ser Gly Gly Ala Gln Ala Thr Ala Trp Ile Val Asp Leu His Asp 
        195                 200                 205             


Glu Tyr Ala Pro Asp Leu Asn Phe Val Gly Thr Val Ser Gly Gly Thr 
    210                 215                 220                 


Leu Val Asp Ala Trp Gly Thr Phe Gln Tyr Ile Asp Tyr Pro Lys Val 
225                 230                 235                 240 


Tyr Leu Lys Gly Ser Ile Leu Ile Met Tyr Thr Gly Leu Phe Ser Gly 
                245                 250                 255     


Tyr Pro Ala Gln Phe Glu Val Ile Trp Pro Tyr Ile Glu Pro Val Ile 
            260                 265                 270         


Gln Glu Asn Met Leu Leu Leu Arg Leu Ala Pro Asn Asp Cys Asn Gln 
        275                 280                 285             


Ser Pro Ile Leu Gln Gly Tyr Asn Asn Ser Ile Met Ala Gly Ile His 
    290                 295                 300                 


Val Asp Leu Pro Glu Phe Pro Ala Ser Lys Tyr Ile Phe Gln His Glu 
305                 310                 315                 320 


Ser Leu Leu Ala Asn Tyr Ser Val Val Pro Val Ser Thr Pro Lys Phe 
                325                 330                 335     


Pro Arg Tyr Met Tyr His Gly Gly Ser Asp Glu Leu Ala Lys Leu Ser 
            340                 345                 350         


Leu Val Glu Gln Tyr Val Asp Gln Gln Trp Asn Thr Gly Ala Asn Leu 
        355                 360                 365             


Thr Phe Val Val Tyr Pro Gly Leu Leu His Asp Glu Thr Ala Tyr Arg 
    370                 375                 380                 


Gly Phe Asp Ala Ala Met Asp Trp Leu Asp Ala Gln Leu Asp Ser Gly 
385                 390                 395                 400 


Tyr Leu Pro Pro Val Asn Ser Thr His Thr 
                405                 410 


<210>  3
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  3
cagcgctggg attcatctgc tc                                              22


<210>  4
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  4
gctaagcagc cttgggagtt tc                                              22


<210>  5
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  5
tacaattggc ctataaggct aaagaaagta                                      30


<210>  6
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  6
atggcgccga tgccgaggaa ctgtcattgc                                      30


<210>  7
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  7
cagacgcatt ggctgccttc                                                 20


<210>  8
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  8
actgccatca tggttcaacc tcac                                            24


<210>  9
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  9
tactgctctg ccgatcgttg                                                 20


<210>  10
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  10
taaagaaacg aagggcccag cagtc                                           25


<210>  11
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  11
agaacaaggc cgagtatgtc                                                 20


<210>  12
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  12
gtcagattag cctccgacat ag                                              22


<210>  13
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  13
ctggcaaatc actaggtgct tagggtgcgt gtg                                  33


<210>  14
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  14
gaaaaaaaca gagccagcat tttttctggt ttggaggacc ttgggtag                  48


<210>  15
<211>  41
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  15
ggtcctccaa accagaaaaa atgctggctc tgtttttttc g                         41


<210>  16
<211>  44
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  16
tgccctgcgg ggatcttcac tctaagaaat cctccgagga aatc                      44


<210>  17
<211>  121
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  17
cagcactggc cgatacccaa agtatataat gcgccgttga acggttatag tcggtcaagc     60

tcttaaagaa agacttaaca acaaaaacaa ctctacacaa atgctggctc tgtttttttc    120

g                                                                    121


<210>  18
<211>  117
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  18
cattgcaggg tgtgcgtcta cgatataaca cgatcgacat cgcgctgggt atgcttctgg     60

gtaaggggtc gcaacgtgtg agttgtcagc actggccgat acccaaagta tataatg       117


<210>  19
<211>  116
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  19
acaagttttt aacatgggtt ttgatttata ttgttttata tgagcgcctc acatatgcgc     60

tgacagccta ttaggagaaa ttcatgtatg agttgagttt acaggtggaa ggtatc        116


<210>  20
<211>  120
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  20
gactttggtc gatatgacga gaatcaggca ataatagctt aagctgaagt gtttttagat     60

ttagttcgga gtgcgcttct caaaagtgct gggatcaaca agtttttaac atgggttttg    120


<210>  21
<211>  9
<212>  PRT
<213>  Candida bombicola

<400>  21

Lys Asn Pro Ser Pro Glu Leu Lys Leu 
1               5                   


<210>  22
<211>  9
<212>  PRT
<213>  Candida bombicola

<400>  22

Lys Val Val Gln Val Ala Tyr Lys Thr 
1               5                   


<210>  23
<211>  13
<212>  PRT
<213>  Candida bombicola

<400>  23

Arg Gln Glu Gly Tyr Ala Gly Leu Asp Gly Ile Arg Ala 
1               5                   10              


<210>  24
<211>  14
<212>  PRT
<213>  Candida bombicola

<400>  24

Arg Tyr Met Tyr His Gly Gly Ser Asp Glu Leu Ala Lys Leu 
1               5                   10                  


<210>  25
<211>  14
<212>  PRT
<213>  Candida bombicola

<400>  25

Arg Tyr Met Tyr His Gly Gly Ser Asp Glu Leu Ala Lys Leu 
1               5                   10                  


<210>  26
<211>  19
<212>  PRT
<213>  Candida bombicola

<400>  26

Arg Glu Leu Leu Thr Leu Pro Asp Ile Phe Asp Phe Gly Pro Asn Leu 
1               5                   10                  15      


Glu Lys Val 
            


<210>  27
<211>  19
<212>  PRT
<213>  Candida bombicola

<400>  27

Arg Glu Leu Leu Thr Leu Pro Asp Ile Phe Asp Phe Gly Pro Asn Leu 
1               5                   10                  15      


Glu Lys Val 
            


<210>  28
<211>  20
<212>  PRT
<213>  Candida bombicola

<400>  28

Lys Arg Glu Leu Leu Thr Leu Pro Asp Ile Phe Asp Phe Gly Pro Asn 
1               5                   10                  15      


Leu Glu Lys Val 
            20  


<210>  29
<211>  21
<212>  PRT
<213>  Candida bombicola

<400>  29

Arg Lys Arg Glu Leu Leu Thr Leu Pro Asp Ile Phe Asp Phe Gly Pro 
1               5                   10                  15      


Asn Leu Glu Lys Val 
            20      


<210>  30
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  30
cccgcggatg ctggctctgt ttttttc                                         27


<210>  31
<211>  39
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  31
ttctagatgt atgagttgag tttacaggtg gaaggtatc                            39


<210>  32
<211>  1218
<212>  DNA
<213>  Candida bombicola

<400>  32
gcaggactcc ctttaggcta tactgcggcc cccgctgaat cattctattt ttggccagag     60

aacatatcca gcctccaagc tggcgagatt tttagaaaac gggaactctt aactctccca    120

gacatctttg actttggccc taatctggaa aaggtcgtac aagtggctta caaaacccgt    180

ctcaccgatg gcaatgactc gttttccatc gccagtatct ttatccctaa gaatccaagc    240

ccagaactca aactttactc ttatcagacg tttgaggatg ccgtgcagct tgattgtgcc    300

ccaagctatg ctttagaagt gggtaacaag tccagcaact atcttcctgt cactagcaat    360

ttatctgcca tcagtcgaga acttgagaaa ggacgtcact gcattatccc tgatcacgag    420

ggctatattt caggattctt tgcaggacgg caggagggat atgctggttt agacggaatt    480

cgcgctgctc gaaactatct caatggcacc aacgagaccc caattggtat cttcggatac    540

agtggaggtg cacaagcaac ggcctggatt gttgatttgc atgacgagta tgctcctgac    600

ttgaactttg ttggaacagt ttctggaggc actttggttg acgcttgggg cacttttcag    660

tatatcgact atccgaaggt gtatctaaag ggcagcattc ttatcatgta tacgggtctt    720

ttttcaggtt atccagctca atttgaggtg atttggccat atattgagcc tgtaattcaa    780

gaaaacatgc tactgctacg tttggcgccg aatgattgta accaaagccc gatacttcaa    840

ggttacaaca attcaatcat ggccggtata catgtggacc ttcccgaatt ccctgcttct    900

aagtacatat tccagcacga gtccctcctt gccaactaca gcgtagtgcc agtttccaca    960

ccgaagtttc ctcgctacat gtaccatggt ggatctgatg agttggccaa attgagcctt   1020

gtcgagcagt atgttgatca acaatggaat accggcgcta atctcacctt cgtggtgtat   1080

ccgggtcttc ttcatgacga gacggcttac cgtggctttg atgccgcgat ggattggctt   1140

gatgcccagc tcgatagtgg ataccttcca cctgtaaact caactcatac agtcgaccat   1200

catcaccatc accactag                                                 1218


<210>  33
<211>  405
<212>  PRT
<213>  Candida bombicola

<400>  33

Ala Gly Leu Pro Leu Gly Tyr Thr Ala Ala Pro Ala Glu Ser Phe Tyr 
1               5                   10                  15      


Phe Trp Pro Glu Asn Ile Ser Ser Leu Gln Ala Gly Glu Ile Phe Arg 
            20                  25                  30          


Lys Arg Glu Leu Leu Thr Leu Pro Asp Ile Phe Asp Phe Gly Pro Asn 
        35                  40                  45              


Leu Glu Lys Val Val Gln Val Ala Tyr Lys Thr Arg Leu Thr Asp Gly 
    50                  55                  60                  


Asn Asp Ser Phe Ser Ile Ala Ser Ile Phe Ile Pro Lys Asn Pro Ser 
65                  70                  75                  80  


Pro Glu Leu Lys Leu Tyr Ser Tyr Gln Thr Phe Glu Asp Ala Val Gln 
                85                  90                  95      


Leu Asp Cys Ala Pro Ser Tyr Ala Leu Glu Val Gly Asn Lys Ser Ser 
            100                 105                 110         


Asn Tyr Leu Pro Val Thr Ser Asn Leu Ser Ala Ile Ser Arg Glu Leu 
        115                 120                 125             


Glu Lys Gly Arg His Cys Ile Ile Pro Asp His Glu Gly Tyr Ile Ser 
    130                 135                 140                 


Gly Phe Phe Ala Gly Arg Gln Glu Gly Tyr Ala Gly Leu Asp Gly Ile 
145                 150                 155                 160 


Arg Ala Ala Arg Asn Tyr Leu Asn Gly Thr Asn Glu Thr Pro Ile Gly 
                165                 170                 175     


Ile Phe Gly Tyr Ser Gly Gly Ala Gln Ala Thr Ala Trp Ile Val Asp 
            180                 185                 190         


Leu Tyr Asp Glu Tyr Ala Pro Asp Leu Asn Phe Val Gly Thr Val Ser 
        195                 200                 205             


Gly Gly Thr Leu Val Asp Ala Trp Gly Thr Phe Gln Tyr Ile Asp Tyr 
    210                 215                 220                 


Pro Lys Val Tyr Leu Lys Gly Ser Ile Leu Ile Met Tyr Thr Gly Leu 
225                 230                 235                 240 


Phe Ser Gly Tyr Pro Ala Gln Phe Glu Val Ile Trp Pro Tyr Ile Glu 
                245                 250                 255     


Pro Val Ile Gln Glu Asn Met Leu Leu Leu Arg Leu Ala Pro Asn Asp 
            260                 265                 270         


Cys Asn Gln Ser Pro Ile Leu Gln Gly Tyr Asn Asn Ser Ile Met Ala 
        275                 280                 285             


Gly Ile His Val Asp Leu Pro Glu Phe Pro Ala Ser Lys Tyr Ile Phe 
    290                 295                 300                 


Gln His Glu Ser Leu Leu Ala Asn Tyr Ser Val Val Pro Val Ser Thr 
305                 310                 315                 320 


Pro Lys Phe Pro Arg Tyr Met Tyr His Gly Gly Ser Asp Glu Leu Ala 
                325                 330                 335     


Lys Leu Ser Leu Val Glu Gln Tyr Val Asp Gln Gln Trp Asn Thr Gly 
            340                 345                 350         


Ala Asn Leu Thr Phe Val Val Tyr Pro Gly Leu Leu His Asp Glu Thr 
        355                 360                 365             


Ala Tyr Arg Gly Phe Asp Ala Ala Met Asp Trp Leu Asp Ala Gln Leu 
    370                 375                 380                 


Asp Ser Gly Tyr Leu Pro Pro Val Asn Ser Thr His Thr Val Asp His 
385                 390                 395                 400 


His His His His His 
                405 


<210>  34
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  34
ccatactcaa gcgcgaacac                                                 20


<210>  35
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  35
gagctcaaga cgcgtttact caatgc                                          26


<210>  36
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  36
cgtcgactgt atgagttgag t                                               21


<210>  37
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  37
gctgcaggac tccctttagg cc                                              22


<210>  38
<211>  12
<212>  PRT
<213>  Candida bombicola

<400>  38

Ala Gly Leu Pro Leu Gly Tyr Thr Ala Ala Pro Ala 
1               5                   10          


<210>  39
<211>  11
<212>  PRT
<213>  Candida bombicola

<400>  39

Lys Val Val Gln Val Ala Tyr Lys Thr Arg Leu 
1               5                   10      


<210>  40
<211>  16
<212>  PRT
<213>  Candida bombicola

<400>  40

Arg Gln Glu Gly Tyr Ala Gly Leu Asp Gly Ile Arg Ala Ala Arg Asn 
1               5                   10                  15      


<210>  41
<211>  18
<212>  PRT
<213>  Candida bombicola

<400>  41

Lys Ser Ser Asn Tyr Leu Pro Val Thr Ser Asn Leu Ser Ala Ile Ser 
1               5                   10                  15      


Arg Glu 
        


<210>  42
<211>  19
<212>  PRT
<213>  Candida bombicola

<400>  42

Arg Leu Thr Asp Gly Asn Asp Ser Phe Ser Ile Ala Ser Ile Phe Ile 
1               5                   10                  15      


Pro Lys Asn 
            


<210>  43
<211>  20
<212>  PRT
<213>  Candida bombicola

<400>  43

Arg His Cys Ile Ile Pro Asp His Glu Gly Tyr Ile Ser Gly Phe Phe 
1               5                   10                  15      


Ala Gly Arg Gln 
            20  


<210>  44
<211>  6
<212>  PRT
<213>  Candida bombicola

<400>  44

Gly Tyr Ser Gly Gly Ala 
1               5       


<210>  45
<211>  22
<212>  PRT
<213>  Candida bombicola

<400>  45

Lys Gly Arg His Cys Ile Ile Pro Asp His Glu Gly Tyr Ile Ser Gly 
1               5                   10                  15      


Phe Phe Ala Gly Arg Gln 
            20          


<210>  46
<211>  26
<212>  PRT
<213>  Candida bombicola

<400>  46

Arg Leu Thr Asp Gly Asn Asp Ser Phe Ser Ile Ala Ser Ile Phe Ile 
1               5                   10                  15      


Pro Lys Asn Pro Ser Pro Glu Leu Lys Leu 
            20                  25      


<210>  47
<211>  26
<212>  PRT
<213>  Candida bombicola

<400>  47

Arg Glu Leu Leu Thr Leu Pro Asp Ile Phe Asp Phe Gly Pro Asn Leu 
1               5                   10                  15      


Glu Lys Val Val Gln Val Ala Tyr Lys Thr 
            20                  25      


<210>  48
<211>  28
<212>  PRT
<213>  Candida bombicola

<400>  48

Lys Thr Arg Leu Thr Asp Gly Asn Asp Ser Phe Ser Ile Ala Ser Ile 
1               5                   10                  15      


Phe Ile Pro Lys Asn Pro Ser Pro Glu Leu Lys Leu 
            20                  25              


