               SEQUENCE LISTING

<110> Glycom A/S

<120> Synthesis of N-acetyl D-neuraminic acid

<150> GB1121615.7
<151> 2011-12-15

<160> 1

<170> BiSSAP 1.0

<210> 1
<211> 297
<212> PRT
<213> Escherichia coli

<220> 
<221> SOURCE
<222> 1..297
<223> /mol_type="protein"
      /note="Strain K-12 N-acetyl neuraminate lyase EC=4.1.3.3"
      /organism="Escherichia coli"

<400> 1
Met Ala Thr Asn Leu Arg Gly Val Met Ala Ala Leu Leu Thr Pro Phe 
1               5                   10                   15    
Asp Gln Gln Gln Ala Leu Asp Lys Ala Ser Leu Arg Arg Leu Val Gln 
            20                   25                  30        
Phe Asn Ile Gln Gln Gly Ile Asp Gly Leu Tyr Val Gly Gly Ser Thr 
        35                   40                  45            
Gly Glu Ala Phe Val Gln Ser Leu Ser Glu Arg Glu Gln Val Leu Glu 
    50                   55                  60                
Ile Val Ala Glu Glu Ala Lys Gly Lys Ile Lys Leu Ile Ala His Val 
65                   70                  75                  80
Gly Cys Val Ser Thr Ala Glu Ser Gln Gln Leu Ala Ala Ser Ala Lys 
                85                   90                  95    
Arg Tyr Gly Phe Asp Ala Val Ser Ala Val Thr Pro Phe Tyr Tyr Pro 
            100                  105                110        
Phe Ser Phe Glu Glu His Cys Asp His Tyr Arg Ala Ile Ile Asp Ser 
        115                  120                125            
Ala Asp Gly Leu Pro Met Val Val Tyr Asn Ile Pro Ala Leu Ser Gly 
    130                  135                140                
Val Lys Leu Thr Leu Asp Gln Ile Asn Thr Leu Val Thr Leu Pro Gly 
145                  150                155                  160
Val Gly Ala Leu Lys Gln Thr Ser Gly Asp Leu Tyr Gln Met Glu Gln 
                165                  170                175    
Ile Arg Arg Glu His Pro Asp Leu Val Leu Tyr Asn Gly Tyr Asp Glu 
            180                  185                190        
Ile Phe Ala Ser Gly Leu Leu Ala Gly Ala Asp Gly Gly Ile Gly Ser 
        195                  200                205            
Thr Tyr Asn Ile Met Gly Trp Arg Tyr Gln Gly Ile Val Lys Ala Leu 
    210                  215                220                
Lys Glu Gly Asp Ile Gln Thr Ala Gln Lys Leu Gln Thr Glu Cys Asn 
225                  230                235                  240
Lys Val Ile Asp Leu Leu Ile Lys Thr Gly Val Phe Arg Gly Leu Lys 
                245                  250                255    
Thr Val Leu His Tyr Met Asp Val Val Ser Val Pro Leu Cys Arg Lys 
            260                  265                270        
Pro Phe Gly Pro Val Asp Glu Lys Tyr Leu Pro Glu Leu Lys Ala Leu 
        275                  280                285            
Ala Gln Gln Leu Met Gln Glu Arg Gly 
    290                  295        

