                         SEQUENCE LISTING

<110>  QUEEN MARY & WESTFIELD COLLEGE, UNIVERSITY OF LONDON
 
<120>  USE OF ANTIBODY

<130>  P53468WO

<150>  GB1121564.7
<151>  2011-12-14

<160>  20    

<170>  PatentIn version 3.3

<210>  1
<211>  25
<212>  PRT
<213>  Homo sapiens


<220>
<221>  ACETYLATION
<222>  (1)..(1)

<400>  1

Ala Met Val Ser Glu Phe Leu Lys Gln Ala Trp Phe Ile Glu Asn Glu 
1               5                   10                  15      


Glu Gln Glu Tyr Val Gln Thr Val Lys 
            20                  25  


<210>  2
<211>  11
<212>  PRT
<213>  Unknown

<220>
<223>  VLCDR1

<400>  2

Lys Ala Ser Glu Asn Val Val Thr Tyr Val Ser 
1               5                   10      


<210>  3
<211>  7
<212>  PRT
<213>  Unknown

<220>
<223>  VLCDR2

<400>  3

Gly Ala Ser Asn Arg Tyr Thr 
1               5           


<210>  4
<211>  9
<212>  PRT
<213>  Unknown

<220>
<223>  VLCDR3

<400>  4

Gly Gln Gly Tyr Ser Tyr Pro Tyr Thr 
1               5                   


<210>  5
<211>  10
<212>  PRT
<213>  Unknown

<220>
<223>  VHCDR1

<400>  5

Gly Tyr Thr Phe Thr Asn Tyr Trp Ile Gly 
1               5                   10  


<210>  6
<211>  17
<212>  PRT
<213>  Unknown

<220>
<223>  VHCDR2

<400>  6

Asp Ile Tyr Pro Gly Gly Asp Tyr Thr Asn Tyr Asn Glu Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  7
<211>  9
<212>  PRT
<213>  Unknown

<220>
<223>  VHCDR3

<400>  7

Trp Gly Leu Gly Tyr Tyr Phe Asp Tyr 
1               5                   


<210>  8
<211>  346
<212>  PRT
<213>  Homo sapiens

<400>  8

Met Ala Met Val Ser Glu Phe Leu Lys Gln Ala Trp Phe Ile Glu Asn 
1               5                   10                  15      


Glu Glu Gln Glu Tyr Val Gln Thr Val Lys Ser Ser Lys Gly Gly Pro 
            20                  25                  30          


Gly Ser Ala Val Ser Pro Tyr Pro Thr Phe Asn Pro Ser Ser Asp Val 
        35                  40                  45              


Ala Ala Leu His Lys Ala Ile Met Val Lys Gly Val Asp Glu Ala Thr 
    50                  55                  60                  


Ile Ile Asp Ile Leu Thr Lys Arg Asn Asn Ala Gln Arg Gln Gln Ile 
65                  70                  75                  80  


Lys Ala Ala Tyr Leu Gln Glu Thr Gly Lys Pro Leu Asp Glu Thr Leu 
                85                  90                  95      


Lys Lys Ala Leu Thr Gly His Leu Glu Glu Val Val Leu Ala Leu Leu 
            100                 105                 110         


Lys Thr Pro Ala Gln Phe Asp Ala Asp Glu Leu Arg Ala Ala Met Lys 
        115                 120                 125             


Gly Leu Gly Thr Asp Glu Asp Thr Leu Ile Glu Ile Leu Ala Ser Arg 
    130                 135                 140                 


Thr Asn Lys Glu Ile Arg Asp Ile Asn Arg Val Tyr Arg Glu Glu Leu 
145                 150                 155                 160 


Lys Arg Asp Leu Ala Lys Asp Ile Thr Ser Asp Thr Ser Gly Asp Phe 
                165                 170                 175     


Arg Asn Ala Leu Leu Ser Leu Ala Lys Gly Asp Arg Ser Glu Asp Phe 
            180                 185                 190         


Gly Val Asn Glu Asp Leu Ala Asp Ser Asp Ala Arg Ala Leu Tyr Glu 
        195                 200                 205             


Ala Gly Glu Arg Arg Lys Gly Thr Asp Val Asn Val Phe Asn Thr Ile 
    210                 215                 220                 


Leu Thr Thr Arg Ser Tyr Pro Gln Leu Arg Arg Val Phe Gln Lys Tyr 
225                 230                 235                 240 


Thr Lys Tyr Ser Lys His Asp Met Asn Lys Val Leu Asp Leu Glu Leu 
                245                 250                 255     


Lys Gly Asp Ile Glu Lys Cys Leu Thr Ala Ile Val Lys Cys Ala Thr 
            260                 265                 270         


Ser Lys Pro Ala Phe Phe Ala Glu Lys Leu His Gln Ala Met Lys Gly 
        275                 280                 285             


Val Gly Thr Arg His Lys Ala Leu Ile Arg Ile Met Val Ser Arg Ser 
    290                 295                 300                 


Glu Ile Asp Met Asn Asp Ile Lys Ala Phe Tyr Gln Lys Met Tyr Gly 
305                 310                 315                 320 


Ile Ser Leu Cys Gln Ala Ile Leu Asp Glu Thr Lys Gly Asp Tyr Glu 
                325                 330                 335     


Lys Ile Leu Val Ala Leu Cys Gly Gly Asn 
            340                 345     


<210>  9
<211>  1041
<212>  DNA
<213>  Homo sapiens

<400>  9
atggcaatgg tatcagaatt cctcaagcag gcctggttta ttgaaaatga agagcaggaa     60

tatgttcaaa ctgtgaagtc atccaaaggt ggtcccggat cagcggtgag cccctatcct    120

accttcaatc catcctcgga tgtcgctgcc ttgcataagg ccataatggt taaaggtgtg    180

gatgaagcaa ccatcattga cattctaact aagcgaaaca atgcacagcg tcaacagatc    240

aaagcagcat atctccagga aacaggaaag cccctggatg aaacactgaa gaaagccctt    300

acaggtcacc ttgaggaggt tgttttggct ctgctaaaaa ctccagcgca atttgatgct    360

gatgaacttc gtgctgccat gaagggcctt ggaactgatg aagatactct aattgagatt    420

ttggcatcaa gaactaacaa agaaatcaga gacattaaca gggtctacag agaggaactg    480

aagagagatc tggccaaaga cataacctca gacacatctg gagattttcg gaacgctttg    540

ctttctcttg ctaagggtga ccgatctgag gactttggtg tgaatgaaga cttggctgat    600

tcagatgcca gggccttgta tgaagcagga gaaaggagaa aggggacaga cgtaaacgtg    660

ttcaatacca tccttaccac cagaagctat ccacaacttc gcagagtgtt tcagaaatac    720

accaagtaca gtaagcatga catgaacaaa gttctggacc tggagttgaa aggtgacatt    780

gagaaatgcc tcacagctat cgtgaagtgc gccacaagca aaccagcttt ctttgcagag    840

aagcttcatc aagccatgaa aggtgttgga actcgccata aggcattgat caggattatg    900

gtttcccgtt ctgaaattga catgaatgat atcaaagcat tctatcagaa gatgtatggt    960

atctcccttt gccaagccat cctggatgaa accaaaggag attatgagaa aatcctggtg   1020

gctctttgtg gaggaaacta a                                             1041


<210>  10
<211>  346
<212>  PRT
<213>  Homo sapiens

<400>  10

Met Ala Met Val Ser Glu Phe Leu Lys Gln Ala Trp Phe Ile Glu Asn 
1               5                   10                  15      


Glu Glu Gln Glu Tyr Val Gln Thr Val Lys Ser Ser Lys Gly Gly Pro 
            20                  25                  30          


Gly Ser Ala Val Ser Pro Tyr Pro Thr Phe Asn Pro Ser Ser Asp Val 
        35                  40                  45              


Ala Ala Leu His Lys Ala Ile Met Val Lys Gly Val Asp Glu Ala Thr 
    50                  55                  60                  


Ile Ile Asp Ile Leu Thr Lys Arg Asn Asn Ala Gln Arg Gln Gln Ile 
65                  70                  75                  80  


Lys Ala Ala Tyr Leu Gln Glu Thr Gly Lys Pro Leu Asp Glu Thr Leu 
                85                  90                  95      


Lys Lys Ala Leu Thr Gly His Leu Glu Glu Val Val Leu Ala Leu Leu 
            100                 105                 110         


Lys Thr Pro Ala Gln Phe Asp Ala Asp Glu Leu Arg Ala Ala Met Lys 
        115                 120                 125             


Gly Leu Gly Thr Asp Glu Asp Thr Leu Ile Glu Ile Leu Ala Ser Arg 
    130                 135                 140                 


Thr Asn Lys Glu Ile Arg Asp Ile Asn Arg Val Tyr Arg Glu Glu Leu 
145                 150                 155                 160 


Lys Arg Asp Leu Ala Lys Asp Ile Thr Ser Asp Thr Ser Gly Asp Phe 
                165                 170                 175     


Arg Asn Ala Leu Leu Ser Leu Ala Lys Gly Asp Arg Ser Glu Asp Phe 
            180                 185                 190         


Gly Val Asn Glu Asp Leu Ala Asp Ser Asp Ala Arg Ala Leu Tyr Glu 
        195                 200                 205             


Ala Gly Glu Arg Arg Lys Gly Thr Asp Val Asn Val Phe Asn Thr Ile 
    210                 215                 220                 


Leu Thr Thr Arg Ser Tyr Pro Gln Leu Arg Arg Val Phe Gln Lys Tyr 
225                 230                 235                 240 


Thr Lys Tyr Ser Lys His Asp Met Asn Lys Val Leu Asp Leu Glu Leu 
                245                 250                 255     


Lys Gly Asp Ile Glu Lys Cys Leu Thr Ala Ile Val Lys Cys Ala Thr 
            260                 265                 270         


Ser Lys Pro Ala Phe Phe Ala Glu Lys Leu His Gln Ala Met Lys Gly 
        275                 280                 285             


Val Gly Thr Arg His Lys Ala Leu Ile Arg Ile Met Val Ser Arg Ser 
    290                 295                 300                 


Glu Ile Asp Met Asn Asp Ile Lys Ala Phe Tyr Gln Lys Met Tyr Gly 
305                 310                 315                 320 


Ile Ser Leu Cys Gln Ala Ile Leu Asp Glu Thr Lys Gly Asp Tyr Glu 
                325                 330                 335     


Lys Ile Leu Val Ala Leu Cys Gly Gly Asn 
            340                 345     


<210>  11
<211>  204
<212>  PRT
<213>  Homo sapiens

<400>  11

Met Asn Leu Ile Leu Arg Tyr Thr Phe Ser Lys Met Ala Met Val Ser 
1               5                   10                  15      


Glu Phe Leu Lys Gln Ala Trp Phe Ile Glu Asn Glu Glu Gln Glu Tyr 
            20                  25                  30          


Val Gln Thr Val Lys Ser Ser Lys Gly Gly Pro Gly Ser Ala Val Ser 
        35                  40                  45              


Pro Tyr Pro Thr Phe Asn Pro Ser Ser Asp Val Ala Ala Leu His Lys 
    50                  55                  60                  


Ala Ile Met Val Lys Gly Val Asp Glu Ala Thr Ile Ile Asp Ile Leu 
65                  70                  75                  80  


Thr Lys Arg Asn Asn Ala Gln Arg Gln Gln Ile Lys Ala Ala Tyr Leu 
                85                  90                  95      


Gln Glu Thr Gly Lys Pro Leu Asp Glu Thr Leu Lys Lys Ala Leu Thr 
            100                 105                 110         


Gly His Leu Glu Glu Val Val Leu Ala Leu Leu Lys Thr Pro Ala Gln 
        115                 120                 125             


Phe Asp Ala Asp Glu Leu Arg Ala Ala Met Lys Gly Leu Gly Thr Asp 
    130                 135                 140                 


Glu Asp Thr Leu Ile Glu Ile Leu Ala Ser Arg Thr Asn Lys Glu Ile 
145                 150                 155                 160 


Arg Asp Ile Asn Arg Val Tyr Arg Glu Glu Leu Lys Arg Asp Leu Ala 
                165                 170                 175     


Lys Asp Ile Thr Ser Asp Thr Ser Gly Asp Phe Arg Asn Ala Leu Leu 
            180                 185                 190         


Ser Leu Ala Lys Gly Asp Arg Ser Glu Asp Phe Gly 
        195                 200                 


<210>  12
<211>  115
<212>  PRT
<213>  Homo sapiens

<400>  12

Met Ala Met Val Ser Glu Phe Leu Lys Gln Ala Trp Phe Ile Glu Asn 
1               5                   10                  15      


Glu Glu Gln Glu Tyr Val Gln Thr Val Lys Ser Ser Lys Gly Gly Pro 
            20                  25                  30          


Gly Ser Ala Val Ser Pro Tyr Pro Thr Phe Asn Pro Ser Ser Asp Val 
        35                  40                  45              


Ala Ala Leu His Lys Ala Ile Met Val Lys Gly Val Asp Glu Ala Thr 
    50                  55                  60                  


Ile Ile Asp Ile Leu Thr Lys Arg Asn Asn Ala Gln Arg Gln Gln Ile 
65                  70                  75                  80  


Lys Ala Ala Tyr Leu Gln Glu Thr Gly Lys Pro Leu Asp Glu Thr Leu 
                85                  90                  95      


Lys Lys Ala Leu Thr Gly His Leu Glu Glu Val Val Leu Ala Leu Leu 
            100                 105                 110         


Lys Thr Pro 
        115 


<210>  13
<211>  321
<212>  DNA
<213>  Artificial

<220>
<223>  light chain variable region of VJ-4B6

<400>  13
aacattgtaa tgacccaatc tcccaaatcc atgtccatgt cagtaggaga gagggtcacc     60

ttgacctgca aggccagtga gaatgtggtt acttatgttt cctggtatca acagaaacca    120

gagcagtctc ctaaactgct gatatacggg gcatccaacc ggtacactgg ggtccccgat    180

cgcttcacag gcagtggatc tgcaacagat ttcactctga ccatcagcag tgtgcaggct    240

gaagaccttg cagattatca ctgtggacag ggttacagct atccgtacac gttcggaggg    300

gggaccaagc tggaaataaa a                                              321


<210>  14
<211>  321
<212>  DNA
<213>  Artificial

<220>
<223>  anti-sense of light chain variable region of VJ-4B6

<400>  14
ttgtaacatt actgggttag agggtttagg tacaggtaca gtcatcctct ctcccagtgg     60

aactggacgt tccggtcact cttacaccaa tgaatacaaa ggaccatagt tgtctttggt    120

ctcgtcagag gatttgacga ctatatgccc cgtaggttgg ccatgtgacc ccaggggcta    180

gcgaagtgtc cgtcacctag acgttgtcta aagtgagact ggtagtcgtc acacgtccga    240

cttctggaac gtctaatagt gacacctgtc ccaatgtcga taggcatgtg caagcctccc    300

ccctggttcg acctttattt t                                              321


<210>  15
<211>  107
<212>  PRT
<213>  Artificial

<220>
<223>  amino acid sequence of the light chain variable region of VJ-4B6

<400>  15

Asn Ile Val Met Thr Gln Ser Pro Lys Ser Met Ser Met Ser Val Gly 
1               5                   10                  15      


Glu Arg Val Thr Leu Thr Cys Lys Ala Ser Glu Asn Val Val Thr Tyr 
            20                  25                  30          


Val Ser Trp Tyr Gln Gln Lys Pro Glu Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Gly Ala Ser Asn Arg Tyr Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Ser Ala Thr Asp Phe Thr Leu Thr Ile Ser Ser Val Gln Ala 
65                  70                  75                  80  


Glu Asp Leu Ala Asp Tyr His Cys Gly Gln Gly Tyr Ser Tyr Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  16
<211>  115
<212>  PRT
<213>  Artificial

<220>
<223>  amino acid sequence of light chain variable region of VJ-4B6

<400>  16

Asn Ile Val Met Thr Gln Ser Pro Lys Ser Met Ser Met Ser Val Gly 
1               5                   10                  15      


Glu Arg Val Thr Leu Thr Cys Lys Ala Ser Glu Asn Val Val Thr Tyr 
            20                  25                  30          


Val Ser Trp Tyr Gln Gln Lys Pro Glu Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Gly Ala Ser Asn Arg Tyr Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Ser Ala Thr Asp Phe Thr Leu Thr Ile Ser Ser Val Gln Ala 
65                  70                  75                  80  


Glu Asp Leu Ala Asp Tyr His Cys Gly Gln Gly Tyr Ser Tyr Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Asp Ala Ala 
            100                 105                 110         


Pro Thr Val 
        115 


<210>  17
<211>  354
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain variable region of VJ-4B6

<400>  17
caggtccagc tgcagcagtc tggacctgaa ctggtcaggc ctgggacttc agtgaagatg     60

tcctgcaagg cttctggata caccttcact aactactgga taggttgggc aaagcagagg    120

cctggacatg gccttgagtg gattggagat atttaccctg gaggtgatta tactaactac    180

aatgagaagt tcaagggcaa ggccacactg actgcagaca aatcctccag cacagcctac    240

atgcagttca gcagcctgac atctgaggac tctgccatct attattgtgc aagatggggg    300

ttaggatact actttgacta ctggggccaa ggcatcactc tcacagtctc ctca          354


<210>  18
<211>  354
<212>  DNA
<213>  Artificial

<220>
<223>  anti sense of heavy chain variable region of VJ-4B6

<400>  18
gtccaggtcg acgtcgtcag acctggactt gaccagtccg gaccctgaag tcacttctac     60

aggacgttcc gaagacctat gtggaagtga ttgatgacct atccaacccg tttcgtctcc    120

ggacctgtac cggaactcac ctaacctcta taaatgggac ctccactaat atgattgatg    180

ttactcttca agttcccgtt ccggtgtgac tgacgtctgt ttaggaggtc gtgtcggatg    240

tacgtcaagt cgtcggactg tagactcctg agacggtaga taataacacg ttctaccccc    300

aatcctatga tgaaactgat gaccccggtt ccgtagtgag agtgtcagag gagt          354


<210>  19
<211>  118
<212>  PRT
<213>  Artificial

<220>
<223>  amino acid sequence of heavy chain variable region of VJ-4B6

<400>  19

Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Arg Pro Gly Thr 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile Gly Trp Ala Lys Gln Arg Pro Gly His Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asp Ile Tyr Pro Gly Gly Asp Tyr Thr Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Phe Ser Ser Leu Thr Ser Glu Asp Ser Ala Ile Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Trp Gly Leu Gly Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly Ile 
            100                 105                 110         


Thr Leu Thr Val Ser Ser 
        115             


<210>  20
<211>  123
<212>  PRT
<213>  Artificial

<220>
<223>  amino acid sequence of heavy chain variable region of VJ-4B6

<400>  20

Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Arg Pro Gly Thr 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile Gly Trp Ala Lys Gln Arg Pro Gly His Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asp Ile Tyr Pro Gly Gly Asp Tyr Thr Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Phe Ser Ser Leu Thr Ser Glu Asp Ser Ala Ile Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Trp Gly Leu Gly Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly Ile 
            100                 105                 110         


Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Pro 
        115                 120             


