                         SEQUENCE LISTING

<110>  FUNDACI PRIVADA CENTRE DE REGULACI GENMICA (CRG)
       INSTITUCI CATALANA DE RECERCA I ESTUDIS AVANATS (ICREA)
 
<120>  METHODS FOR PRODUCING ENGINEERED FLUORESCENT PROTEINS FOR 
       ENHANCED FRET, PRODUCTS AND USES THEREOF

<130>  BAR/FMOD/398

<150>  GB 1121570.4
<151>  2011-12-15

<160>  98    

<170>  PatentIn version 3.5

<210>  1
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCitrine

<400>  1

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  2
<211>  235
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCherry

<400>  2

Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met 
1               5                   10                  15      


Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu 
            20                  25                  30          


Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala 
        35                  40                  45              


Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile 
    50                  55                  60                  


Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro 
65                  70                  75                  80  


Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys 
                85                  90                  95      


Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr 
            100                 105                 110         


Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu 
        115                 120                 125             


Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr 
    130                 135                 140                 


Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala 
145                 150                 155                 160 


Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His 
                165                 170                 175     


Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln 
            180                 185                 190         


Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser His 
        195                 200                 205             


Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg 
    210                 215                 220                 


His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235 


<210>  3
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Citrine

<400>  3

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  4
<211>  232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mTFP1

<400>  4

Val Ser Lys Gly Glu Glu Gly Val Ile Lys Pro Asp Met Lys Ile Lys 
1               5                   10                  15      


Leu Lys Met Glu Gly Asn Val Asn Gly His Ala Phe Val Ile Glu Gly 
            20                  25                  30          


Glu Gly Glu Gly Lys Pro Tyr Asp Gly Thr Asn Thr Ile Asn Leu Glu 
        35                  40                  45              


Val Lys Glu Gly Ala Pro Leu Pro Phe Ser Tyr Asp Ile Leu Thr Thr 
    50                  55                  60                  


Ala Phe Ala Tyr Gly Asn Arg Ala Phe Thr Lys Tyr Pro Asp Asp Ile 
65                  70                  75                  80  


Pro Asn Tyr Phe Lys Gln Ser Phe Pro Glu Gly Tyr Ser Trp Glu Arg 
                85                  90                  95      


Thr Met Thr Phe Glu Asp Lys Gly Ile Val Lys Val Lys Ser Asp Ile 
            100                 105                 110         


Ser Met Glu Glu Asp Ser Phe Ile Tyr Glu Ile His Leu Lys Gly Glu 
        115                 120                 125             


Asn Phe Pro Pro Asn Gly Pro Val Met Gln Lys Lys Thr Thr Gly Trp 
    130                 135                 140                 


Asp Ala Ser Thr Glu Arg Met Tyr Val Arg Asp Gly Val Leu Lys Gly 
145                 150                 155                 160 


Asp Val Lys His Lys Leu Leu Leu Glu Gly Gly Gly His His Arg Val 
                165                 170                 175     


Asp Phe Lys Thr Ile Tyr Arg Ala Lys Lys Ala Val Lys Leu Pro Asp 
            180                 185                 190         


Tyr His Phe Val Asp His Arg Ile Glu Ile Leu Asn His Asp Lys Asp 
        195                 200                 205             


Tyr Asn Lys Val Thr Val Tyr Glu Ser Ala Val Ala Arg Asn Ser Thr 
    210                 215                 220                 


Asp Gly Met Asp Glu Leu Tyr Lys 
225                 230         


<210>  5
<211>  70
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SH3 domain

<400>  5

Asp Asp Asn Phe Ile Tyr Lys Ala Lys Ala Leu Tyr Pro Tyr Asp Ala 
1               5                   10                  15      


Asp Asp Asp Asp Ala Tyr Glu Ile Ser Phe Glu Gln Asn Glu Ile Leu 
            20                  25                  30          


Gln Val Ser Asp Ile Glu Gly Arg Trp Trp Lys Ala Arg Arg Ala Asn 
        35                  40                  45              


Gly Glu Thr Gly Ile Ile Pro Ser Asn Tyr Val Gln Leu Ile Asp Gly 
    50                  55                  60                  


Pro Glu Glu Met His Arg 
65                  70  


<210>  6
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  WW domain

<400>  6

Phe Glu Ile Pro Asp Asp Val Pro Leu Pro Ala Gly Trp Glu Met Ala 
1               5                   10                  15      


Lys Thr Ser Ser Gly Gln Arg Tyr Phe Lys Asn His Ile Asp Gln Thr 
            20                  25                  30          


Thr Thr Trp Gln Asp Pro Arg Lys Ala Met Leu Ser Gln Met 
        35                  40                  45      


<210>  7
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SP1

<400>  7

Ile Pro Ser Lys Pro Leu Pro Pro Leu Pro Val 
1               5                   10      


<210>  8
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SP2

<400>  8

Ile Val Asn Lys Pro Leu Ala Pro Leu Pro Val 
1               5                   10      


<210>  9
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  WP1

<400>  9

Gly Thr Pro Pro Pro Pro Tyr Thr Val Gly 
1               5                   10  


<210>  10
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  WP2

<400>  10

Pro Leu Pro Pro Tyr Thr 
1               5       


<210>  11
<211>  310
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mcitrine - long spacer - SH3

<400>  11

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Ser Gly 
225                 230                 235                 240 


Asp Asp Asn Phe Ile Tyr Lys Ala Lys Ala Leu Tyr Pro Tyr Asp Ala 
                245                 250                 255     


Asp Asp Asp Asp Ala Tyr Glu Ile Ser Phe Glu Gln Asn Glu Ile Leu 
            260                 265                 270         


Gln Val Ser Asp Ile Glu Gly Arg Trp Trp Lys Ala Arg Arg Ala Asn 
        275                 280                 285             


Gly Glu Thr Gly Ile Ile Pro Ser Asn Tyr Val Gln Leu Ile Asp Gly 
    290                 295                 300                 


Pro Glu Glu Met His Arg 
305                 310 


<210>  12
<211>  304
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mcitrine - short spacer - SH3

<400>  12

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr Leu Gly Ser Gly Asp Asp Asn Phe Ile Tyr 
225                 230                 235                 240 


Lys Ala Lys Ala Leu Tyr Pro Tyr Asp Ala Asp Asp Asp Asp Ala Tyr 
                245                 250                 255     


Glu Ile Ser Phe Glu Gln Asn Glu Ile Leu Gln Val Ser Asp Ile Glu 
            260                 265                 270         


Gly Arg Trp Trp Lys Ala Arg Arg Ala Asn Gly Glu Thr Gly Ile Ile 
        275                 280                 285             


Pro Ser Asn Tyr Val Gln Leu Ile Asp Gly Pro Glu Glu Met His Arg 
    290                 295                 300                 


<210>  13
<211>  248
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCherry - long spacer - strong peptide for SH3

<400>  13

Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met 
1               5                   10                  15      


Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu 
            20                  25                  30          


Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala 
        35                  40                  45              


Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile 
    50                  55                  60                  


Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro 
65                  70                  75                  80  


Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys 
                85                  90                  95      


Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr 
            100                 105                 110         


Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu 
        115                 120                 125             


Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr 
    130                 135                 140                 


Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala 
145                 150                 155                 160 


Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His 
                165                 170                 175     


Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln 
            180                 185                 190         


Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser His 
        195                 200                 205             


Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg 
    210                 215                 220                 


His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys Gly Ser Ile Pro Ser 
225                 230                 235                 240 


Lys Pro Leu Pro Pro Leu Pro Val 
                245             


<210>  14
<211>  248
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCherry - long spacer - weak peptide for SH3

<400>  14

Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met 
1               5                   10                  15      


Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu 
            20                  25                  30          


Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala 
        35                  40                  45              


Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile 
    50                  55                  60                  


Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro 
65                  70                  75                  80  


Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys 
                85                  90                  95      


Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr 
            100                 105                 110         


Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu 
        115                 120                 125             


Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr 
    130                 135                 140                 


Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala 
145                 150                 155                 160 


Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His 
                165                 170                 175     


Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln 
            180                 185                 190         


Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser His 
        195                 200                 205             


Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg 
    210                 215                 220                 


His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys Gly Ser Ile Val Asn 
225                 230                 235                 240 


Lys Pro Leu Ala Pro Leu Pro Val 
                245             


<210>  15
<211>  242
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCherry - short spacer - strong peptide for SH3

<400>  15

Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met 
1               5                   10                  15      


Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu 
            20                  25                  30          


Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala 
        35                  40                  45              


Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile 
    50                  55                  60                  


Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro 
65                  70                  75                  80  


Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys 
                85                  90                  95      


Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr 
            100                 105                 110         


Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu 
        115                 120                 125             


Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr 
    130                 135                 140                 


Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala 
145                 150                 155                 160 


Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His 
                165                 170                 175     


Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln 
            180                 185                 190         


Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser His 
        195                 200                 205             


Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg 
    210                 215                 220                 


His Ser Thr Gly Gly Ser Gly Ile Pro Ser Lys Pro Leu Pro Pro Leu 
225                 230                 235                 240 


Pro Val 
        


<210>  16
<211>  242
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCherry - short spacer - weak peptide for SH3

<400>  16

Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met 
1               5                   10                  15      


Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu 
            20                  25                  30          


Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala 
        35                  40                  45              


Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile 
    50                  55                  60                  


Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro 
65                  70                  75                  80  


Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys 
                85                  90                  95      


Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr 
            100                 105                 110         


Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu 
        115                 120                 125             


Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr 
    130                 135                 140                 


Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala 
145                 150                 155                 160 


Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His 
                165                 170                 175     


Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln 
            180                 185                 190         


Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser His 
        195                 200                 205             


Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg 
    210                 215                 220                 


His Ser Thr Gly Gly Ser Gly Ile Val Asn Lys Pro Leu Ala Pro Leu 
225                 230                 235                 240 


Pro Val 
        


<210>  17
<211>  286
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mcitrine - long spacer - WW

<400>  17

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Ser Gly 
225                 230                 235                 240 


Phe Glu Ile Pro Asp Asp Val Pro Leu Pro Ala Gly Trp Glu Met Ala 
                245                 250                 255     


Lys Thr Ser Ser Gly Gln Arg Tyr Phe Lys Asn His Ile Asp Gln Thr 
            260                 265                 270         


Thr Thr Trp Gln Asp Pro Arg Lys Ala Met Leu Ser Gln Met 
        275                 280                 285     


<210>  18
<211>  280
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mcitrine - short spacer - WW

<400>  18

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr Leu Gly Ser Gly Phe Glu Ile Pro Asp Asp 
225                 230                 235                 240 


Val Pro Leu Pro Ala Gly Trp Glu Met Ala Lys Thr Ser Ser Gly Gln 
                245                 250                 255     


Arg Tyr Phe Lys Asn His Ile Asp Gln Thr Thr Thr Trp Gln Asp Pro 
            260                 265                 270         


Arg Lys Ala Met Leu Ser Gln Met 
        275                 280 


<210>  19
<211>  247
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCherry - long spacer - strong peptide for WW

<400>  19

Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met 
1               5                   10                  15      


Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu 
            20                  25                  30          


Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala 
        35                  40                  45              


Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile 
    50                  55                  60                  


Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro 
65                  70                  75                  80  


Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys 
                85                  90                  95      


Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr 
            100                 105                 110         


Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu 
        115                 120                 125             


Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr 
    130                 135                 140                 


Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala 
145                 150                 155                 160 


Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His 
                165                 170                 175     


Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln 
            180                 185                 190         


Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser His 
        195                 200                 205             


Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg 
    210                 215                 220                 


His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys Gly Ser Gly Thr Pro 
225                 230                 235                 240 


Pro Pro Pro Tyr Thr Val Gly 
                245         


<210>  20
<211>  243
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCherry - long spacer - weak peptide for WW

<400>  20

Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met 
1               5                   10                  15      


Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu 
            20                  25                  30          


Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala 
        35                  40                  45              


Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile 
    50                  55                  60                  


Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro 
65                  70                  75                  80  


Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys 
                85                  90                  95      


Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr 
            100                 105                 110         


Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu 
        115                 120                 125             


Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr 
    130                 135                 140                 


Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala 
145                 150                 155                 160 


Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His 
                165                 170                 175     


Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln 
            180                 185                 190         


Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser His 
        195                 200                 205             


Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg 
    210                 215                 220                 


His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys Gly Ser Pro Leu Pro 
225                 230                 235                 240 


Pro Tyr Thr 
            


<210>  21
<211>  241
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCherry - short spacer - strong peptide for WW

<400>  21

Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met 
1               5                   10                  15      


Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu 
            20                  25                  30          


Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala 
        35                  40                  45              


Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile 
    50                  55                  60                  


Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro 
65                  70                  75                  80  


Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys 
                85                  90                  95      


Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr 
            100                 105                 110         


Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu 
        115                 120                 125             


Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr 
    130                 135                 140                 


Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala 
145                 150                 155                 160 


Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His 
                165                 170                 175     


Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln 
            180                 185                 190         


Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser His 
        195                 200                 205             


Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg 
    210                 215                 220                 


His Ser Thr Gly Gly Ser Gly Gly Thr Pro Pro Pro Pro Tyr Thr Val 
225                 230                 235                 240 


Gly 
    


<210>  22
<211>  237
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCherry - short spacer - weak peptide for WW

<400>  22

Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met 
1               5                   10                  15      


Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu 
            20                  25                  30          


Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala 
        35                  40                  45              


Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile 
    50                  55                  60                  


Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro 
65                  70                  75                  80  


Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys 
                85                  90                  95      


Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr 
            100                 105                 110         


Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu 
        115                 120                 125             


Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr 
    130                 135                 140                 


Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala 
145                 150                 155                 160 


Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His 
                165                 170                 175     


Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln 
            180                 185                 190         


Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser His 
        195                 200                 205             


Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg 
    210                 215                 220                 


His Ser Thr Gly Gly Ser Gly Pro Leu Pro Pro Tyr Thr 
225                 230                 235         


<210>  23
<211>  280
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mTFP1 - long spacer WW domain

<400>  23

Val Ser Lys Gly Glu Glu Gly Val Ile Lys Pro Asp Met Lys Ile Lys 
1               5                   10                  15      


Leu Lys Met Glu Gly Asn Val Asn Gly His Ala Phe Val Ile Glu Gly 
            20                  25                  30          


Glu Gly Glu Gly Lys Pro Tyr Asp Gly Thr Asn Thr Ile Asn Leu Glu 
        35                  40                  45              


Val Lys Glu Gly Ala Pro Leu Pro Phe Ser Tyr Asp Ile Leu Thr Thr 
    50                  55                  60                  


Ala Phe Ala Tyr Gly Asn Arg Ala Phe Thr Lys Tyr Pro Asp Asp Ile 
65                  70                  75                  80  


Pro Asn Tyr Phe Lys Gln Ser Phe Pro Glu Gly Tyr Ser Trp Glu Arg 
                85                  90                  95      


Thr Met Thr Phe Glu Asp Lys Gly Ile Val Lys Val Lys Ser Asp Ile 
            100                 105                 110         


Ser Met Glu Glu Asp Ser Phe Ile Tyr Glu Ile His Leu Lys Gly Glu 
        115                 120                 125             


Asn Phe Pro Pro Asn Gly Pro Val Met Gln Lys Lys Thr Thr Gly Trp 
    130                 135                 140                 


Asp Ala Ser Thr Glu Arg Met Tyr Val Arg Asp Gly Val Leu Lys Gly 
145                 150                 155                 160 


Asp Val Lys His Lys Leu Leu Leu Glu Gly Gly Gly His His Arg Val 
                165                 170                 175     


Asp Phe Lys Thr Ile Tyr Arg Ala Lys Lys Ala Val Lys Leu Pro Asp 
            180                 185                 190         


Tyr His Phe Val Asp His Arg Ile Glu Ile Leu Asn His Asp Lys Asp 
        195                 200                 205             


Tyr Asn Lys Val Thr Val Tyr Glu Ser Ala Val Ala Arg Asn Ser Thr 
    210                 215                 220                 


Asp Gly Met Asp Glu Leu Tyr Lys Ser Gly Phe Glu Ile Pro Asp Asp 
225                 230                 235                 240 


Val Pro Leu Pro Ala Gly Trp Glu Met Ala Lys Thr Ser Ser Gly Gln 
                245                 250                 255     


Arg Tyr Phe Lys Asn His Ile Asp Gln Thr Thr Thr Trp Gln Asp Pro 
            260                 265                 270         


Arg Lys Ala Met Leu Ser Gln Met 
        275                 280 


<210>  24
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FKBP

<400>  24

Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro 
1               5                   10                  15      


Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
            20                  25                  30          


Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe 
        35                  40                  45              


Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala 
    50                  55                  60                  


Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr 
65                  70                  75                  80  


Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr 
                85                  90                  95      


Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu 
            100                 105         


<210>  25
<211>  93
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FRB

<400>  25

Ile Leu Trp His Glu Met Trp His Glu Gly Leu Glu Glu Ala Ser Arg 
1               5                   10                  15      


Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe Glu Val Leu Glu 
            20                  25                  30          


Pro Leu His Ala Met Met Glu Arg Gly Pro Gln Thr Leu Lys Glu Thr 
        35                  40                  45              


Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala Gln Glu Trp 
    50                  55                  60                  


Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp Leu Leu Gln Ala 
65                  70                  75                  80  


Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Lys 
                85                  90              


<210>  26
<211>  648
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Raf1 (c-Raf)

<400>  26

Met Glu His Ile Gln Gly Ala Trp Lys Thr Ile Ser Asn Gly Phe Gly 
1               5                   10                  15      


Phe Lys Asp Ala Val Phe Asp Gly Ser Ser Cys Ile Ser Pro Thr Ile 
            20                  25                  30          


Val Gln Gln Phe Gly Tyr Gln Arg Arg Ala Ser Asp Asp Gly Lys Leu 
        35                  40                  45              


Thr Asp Pro Ser Lys Thr Ser Asn Thr Ile Arg Val Phe Leu Pro Asn 
    50                  55                  60                  


Lys Gln Arg Thr Val Val Asn Val Arg Asn Gly Met Ser Leu His Asp 
65                  70                  75                  80  


Cys Leu Met Lys Ala Leu Lys Val Arg Gly Leu Gln Pro Glu Cys Cys 
                85                  90                  95      


Ala Val Phe Arg Leu Leu His Glu His Lys Gly Lys Lys Ala Arg Leu 
            100                 105                 110         


Asp Trp Asn Thr Asp Ala Ala Ser Leu Ile Gly Glu Glu Leu Gln Val 
        115                 120                 125             


Asp Phe Leu Asp His Val Pro Leu Thr Thr His Asn Phe Ala Arg Lys 
    130                 135                 140                 


Thr Phe Leu Lys Leu Ala Phe Cys Asp Ile Cys Gln Lys Phe Leu Leu 
145                 150                 155                 160 


Asn Gly Phe Arg Cys Gln Thr Cys Gly Tyr Lys Phe His Glu His Cys 
                165                 170                 175     


Ser Thr Lys Val Pro Thr Met Cys Val Asp Trp Ser Asn Ile Arg Gln 
            180                 185                 190         


Leu Leu Leu Phe Pro Asn Ser Thr Ile Gly Asp Ser Gly Val Pro Ala 
        195                 200                 205             


Leu Pro Ser Leu Thr Met Arg Arg Met Arg Glu Ser Val Ser Arg Met 
    210                 215                 220                 


Pro Val Ser Ser Gln His Arg Tyr Ser Thr Pro His Ala Phe Thr Phe 
225                 230                 235                 240 


Asn Thr Ser Ser Pro Ser Ser Glu Gly Ser Leu Ser Gln Arg Gln Arg 
                245                 250                 255     


Ser Thr Ser Thr Pro Asn Val His Met Val Ser Thr Thr Leu Pro Val 
            260                 265                 270         


Asp Ser Arg Met Ile Glu Asp Ala Ile Arg Ser His Ser Glu Ser Ala 
        275                 280                 285             


Ser Pro Ser Ala Leu Ser Ser Ser Pro Asn Asn Leu Ser Pro Thr Gly 
    290                 295                 300                 


Trp Ser Gln Pro Lys Thr Pro Val Pro Ala Gln Arg Glu Arg Ala Pro 
305                 310                 315                 320 


Val Ser Gly Thr Gln Glu Lys Asn Lys Ile Arg Pro Arg Gly Gln Arg 
                325                 330                 335     


Asp Ser Ser Tyr Tyr Trp Glu Ile Glu Ala Ser Glu Val Met Leu Ser 
            340                 345                 350         


Thr Arg Ile Gly Ser Gly Ser Phe Gly Thr Val Tyr Lys Gly Lys Trp 
        355                 360                 365             


His Gly Asp Val Ala Val Lys Ile Leu Lys Val Val Asp Pro Thr Pro 
    370                 375                 380                 


Glu Gln Phe Gln Ala Phe Arg Asn Glu Val Ala Val Leu Arg Lys Thr 
385                 390                 395                 400 


Arg His Val Asn Ile Leu Leu Phe Met Gly Tyr Met Thr Lys Asp Asn 
                405                 410                 415     


Leu Ala Ile Val Thr Gln Trp Cys Glu Gly Ser Ser Leu Tyr Lys His 
            420                 425                 430         


Leu His Val Gln Glu Thr Lys Phe Gln Met Phe Gln Leu Ile Asp Ile 
        435                 440                 445             


Ala Arg Gln Thr Ala Gln Gly Met Asp Tyr Leu His Ala Lys Asn Ile 
    450                 455                 460                 


Ile His Arg Asp Met Lys Ser Asn Asn Ile Phe Leu His Glu Gly Leu 
465                 470                 475                 480 


Thr Val Lys Ile Gly Asp Phe Gly Leu Ala Thr Val Lys Ser Arg Trp 
                485                 490                 495     


Ser Gly Ser Gln Gln Val Glu Gln Pro Thr Gly Ser Val Leu Trp Met 
            500                 505                 510         


Ala Pro Glu Val Ile Arg Met Gln Asp Asn Asn Pro Phe Ser Phe Gln 
        515                 520                 525             


Ser Asp Val Tyr Ser Tyr Gly Ile Val Leu Tyr Glu Leu Met Thr Gly 
    530                 535                 540                 


Glu Leu Pro Tyr Ser His Ile Asn Asn Arg Asp Gln Ile Ile Phe Met 
545                 550                 555                 560 


Val Gly Arg Gly Tyr Ala Ser Pro Asp Leu Ser Lys Leu Tyr Lys Asn 
                565                 570                 575     


Cys Pro Lys Ala Met Lys Arg Leu Val Ala Asp Cys Val Lys Lys Val 
            580                 585                 590         


Lys Glu Glu Arg Pro Leu Phe Pro Gln Ile Leu Ser Ser Ile Glu Leu 
        595                 600                 605             


Leu Gln His Ser Leu Pro Lys Ile Asn Arg Ser Ala Ser Glu Pro Ser 
    610                 615                 620                 


Leu His Arg Ala Ala His Thr Glu Asp Ile Asn Ala Cys Thr Leu Thr 
625                 630                 635                 640 


Thr Ser Pro Arg Leu Pro Val Phe 
                645             


<210>  27
<211>  81
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Raf1-RBD (Ras binding domain)

<400>  27

Ser Lys Thr Ser Asn Thr Ile Arg Val Phe Leu Pro Asn Lys Gln Arg 
1               5                   10                  15      


Thr Val Val Asn Val Arg Asn Gly Met Ser Leu His Asp Cys Leu Met 
            20                  25                  30          


Lys Ala Leu Lys Val Arg Gly Leu Gln Pro Glu Cys Cys Ala Val Phe 
        35                  40                  45              


Arg Leu Leu His Glu His Lys Gly Lys Lys Ala Arg Leu Asp Trp Asn 
    50                  55                  60                  


Thr Asp Ala Ala Ser Leu Ile Gly Glu Glu Leu Gln Val Asp Phe Leu 
65                  70                  75                  80  


Asp 
    


<210>  28
<211>  189
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H-Ras

<400>  28

Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly Gly Val Gly Lys 
1               5                   10                  15      


Ser Ala Leu Thr Ile Gln Leu Ile Gln Asn His Phe Val Asp Glu Tyr 
            20                  25                  30          


Asp Pro Thr Ile Glu Asp Ser Tyr Arg Lys Gln Val Val Ile Asp Gly 
        35                  40                  45              


Glu Thr Cys Leu Leu Asp Ile Leu Asp Thr Ala Gly Gln Glu Glu Tyr 
    50                  55                  60                  


Ser Ala Met Arg Asp Gln Tyr Met Arg Thr Gly Glu Gly Phe Leu Cys 
65                  70                  75                  80  


Val Phe Ala Ile Asn Asn Thr Lys Ser Phe Glu Asp Ile His Gln Tyr 
                85                  90                  95      


Arg Glu Gln Ile Lys Arg Val Lys Asp Ser Asp Asp Val Pro Met Val 
            100                 105                 110         


Leu Val Gly Asn Lys Cys Asp Leu Ala Ala Arg Thr Val Glu Ser Arg 
        115                 120                 125             


Gln Ala Gln Asp Leu Ala Arg Ser Tyr Gly Ile Pro Tyr Ile Glu Thr 
    130                 135                 140                 


Ser Ala Lys Thr Arg Gln Gly Val Glu Asp Ala Phe Tyr Thr Leu Val 
145                 150                 155                 160 


Arg Glu Ile Arg Gln His Lys Leu Arg Lys Leu Asn Pro Pro Asp Glu 
                165                 170                 175     


Ser Gly Pro Gly Cys Met Ser Cys Lys Cys Val Leu Ser 
            180                 185                 


<210>  29
<211>  440
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FKBP-20-mCitrine~WW

<400>  29

Met Thr Gly Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
1               5                   10                  15      


Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
            20                  25                  30          


Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro 
        35                  40                  45              


Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
    50                  55                  60                  


Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
65                  70                  75                  80  


Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
                85                  90                  95      


His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Thr Gly 
            100                 105                 110         


Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
        115                 120                 125             


Gly Ser Gly Ser Thr Gly Val Ser Lys Gly Glu Glu Leu Phe Thr Gly 
    130                 135                 140                 


Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys 
145                 150                 155                 160 


Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu 
                165                 170                 175     


Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro 
            180                 185                 190         


Thr Leu Val Thr Thr Phe Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr 
        195                 200                 205             


Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu 
    210                 215                 220                 


Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr 
225                 230                 235                 240 


Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg 
                245                 250                 255     


Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly 
            260                 265                 270         


His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala 
        275                 280                 285             


Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn 
    290                 295                 300                 


Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr 
305                 310                 315                 320 


Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser 
                325                 330                 335     


Tyr Gln Ser Lys Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met 
            340                 345                 350         


Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp 
        355                 360                 365             


Glu Leu Tyr Lys Ser Gly Phe Glu Ile Pro Asp Asp Val Pro Leu Pro 
    370                 375                 380                 


Ala Gly Trp Glu Met Ala Lys Thr Ser Ser Gly Gln Arg Tyr Phe Lys 
385                 390                 395                 400 


Asn His Ile Asp Gln Thr Thr Thr Trp Gln Asp Pro Arg Lys Ala Met 
                405                 410                 415     


Leu Ser Gln Met Thr Gly Leu Glu Val Leu Phe Gln Gly Pro Thr Gly 
            420                 425                 430         


His His His His His His Thr Gly 
        435                 440 


<210>  30
<211>  377
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FRB-20-mCherry.Wp2

<400>  30

Met Thr Gly Ile Leu Trp His Glu Met Trp His Glu Gly Leu Glu Glu 
1               5                   10                  15      


Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe Glu 
            20                  25                  30          


Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln Thr Leu 
        35                  40                  45              


Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala 
    50                  55                  60                  


Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp Leu 
65                  70                  75                  80  


Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Lys 
                85                  90                  95      


Thr Gly Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
            100                 105                 110         


Gly Ser Gly Ser Gly Ser Thr Gly Val Ser Lys Gly Glu Glu Asp Asn 
        115                 120                 125             


Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys Val His Met Glu Gly 
    130                 135                 140                 


Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu Gly Glu Gly Arg 
145                 150                 155                 160 


Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys Val Thr Lys Gly Gly 
                165                 170                 175     


Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe Met Tyr Gly 
            180                 185                 190         


Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro Asp Tyr Leu Lys 
        195                 200                 205             


Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val Met Asn Phe Glu 
    210                 215                 220                 


Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser Leu Gln Asp Gly 
225                 230                 235                 240 


Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe Pro Ser Asp 
                245                 250                 255     


Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu Ala Ser Ser Glu 
            260                 265                 270         


Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys Gln Arg 
        275                 280                 285             


Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala Glu Val Lys Thr Thr 
    290                 295                 300                 


Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro Gly Ala Tyr Asn Val Asn 
305                 310                 315                 320 


Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr Thr Ile Val Glu 
                325                 330                 335     


Gln Tyr Glu Arg Ala Glu Gly Arg His Ser Thr Gly Gly Ser Gly Pro 
            340                 345                 350         


Leu Pro Pro Tyr Thr Thr Gly Leu Glu Val Leu Phe Gln Gly Pro Thr 
        355                 360                 365             


Gly His His His His His His Thr Gly 
    370                 375         


<210>  31
<211>  436
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FKBP-20-mTFP1~WW

<400>  31

Met Thr Gly Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
1               5                   10                  15      


Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
            20                  25                  30          


Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro 
        35                  40                  45              


Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
    50                  55                  60                  


Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
65                  70                  75                  80  


Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
                85                  90                  95      


His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Thr Gly 
            100                 105                 110         


Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
        115                 120                 125             


Gly Ser Gly Ser Thr Gly Val Ser Lys Gly Glu Glu Gly Val Ile Lys 
    130                 135                 140                 


Pro Asp Met Lys Ile Lys Leu Lys Met Glu Gly Asn Val Asn Gly His 
145                 150                 155                 160 


Ala Phe Val Ile Glu Gly Glu Gly Glu Gly Lys Pro Tyr Asp Gly Thr 
                165                 170                 175     


Asn Thr Ile Asn Leu Glu Val Lys Glu Gly Ala Pro Leu Pro Phe Ser 
            180                 185                 190         


Tyr Asp Ile Leu Thr Thr Ala Phe Ala Tyr Gly Asn Arg Ala Phe Thr 
        195                 200                 205             


Lys Tyr Pro Asp Asp Ile Pro Asn Tyr Phe Lys Gln Ser Phe Pro Glu 
    210                 215                 220                 


Gly Tyr Ser Trp Glu Arg Thr Met Thr Phe Glu Asp Lys Gly Ile Val 
225                 230                 235                 240 


Lys Val Lys Ser Asp Ile Ser Met Glu Glu Asp Ser Phe Ile Tyr Glu 
                245                 250                 255     


Ile His Leu Lys Gly Glu Asn Phe Pro Pro Asn Gly Pro Val Met Gln 
            260                 265                 270         


Lys Lys Thr Thr Gly Trp Asp Ala Ser Thr Glu Arg Met Tyr Val Arg 
        275                 280                 285             


Asp Gly Val Leu Lys Gly Asp Val Lys His Lys Leu Leu Leu Glu Gly 
    290                 295                 300                 


Gly Gly His His Arg Val Asp Phe Lys Thr Ile Tyr Arg Ala Lys Lys 
305                 310                 315                 320 


Ala Val Lys Leu Pro Asp Tyr His Phe Val Asp His Arg Ile Glu Ile 
                325                 330                 335     


Leu Asn His Asp Lys Asp Tyr Asn Lys Val Thr Val Tyr Glu Ser Ala 
            340                 345                 350         


Val Ala Arg Asn Ser Thr Asp Gly Met Asp Glu Leu Tyr Lys Ser Gly 
        355                 360                 365             


Phe Glu Ile Pro Asp Asp Val Pro Leu Pro Ala Gly Trp Glu Met Ala 
    370                 375                 380                 


Lys Thr Ser Ser Gly Gln Arg Tyr Phe Lys Asn His Ile Asp Gln Thr 
385                 390                 395                 400 


Thr Thr Trp Gln Asp Pro Arg Lys Ala Met Leu Ser Gln Met Thr Gly 
                405                 410                 415     


Leu Glu Val Leu Phe Gln Gly Pro Thr Gly Ser Gly His His His His 
            420                 425                 430         


His His Thr Gly 
        435     


<210>  32
<211>  392
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FKBP-20AA-mCitrine

<400>  32

Met Thr Gly Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
1               5                   10                  15      


Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
            20                  25                  30          


Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro 
        35                  40                  45              


Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
    50                  55                  60                  


Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
65                  70                  75                  80  


Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
                85                  90                  95      


His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Thr Gly 
            100                 105                 110         


Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
        115                 120                 125             


Gly Ser Gly Ser Thr Gly Val Ser Lys Gly Glu Glu Leu Phe Thr Gly 
    130                 135                 140                 


Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys 
145                 150                 155                 160 


Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu 
                165                 170                 175     


Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro 
            180                 185                 190         


Thr Leu Val Thr Thr Phe Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr 
        195                 200                 205             


Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu 
    210                 215                 220                 


Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr 
225                 230                 235                 240 


Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg 
                245                 250                 255     


Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly 
            260                 265                 270         


His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala 
        275                 280                 285             


Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn 
    290                 295                 300                 


Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr 
305                 310                 315                 320 


Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser 
                325                 330                 335     


Tyr Gln Ser Lys Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met 
            340                 345                 350         


Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp 
        355                 360                 365             


Glu Leu Tyr Lys Thr Gly Leu Glu Val Leu Phe Gln Gly Pro Thr Gly 
    370                 375                 380                 


His His His His His His Thr Gly 
385                 390         


<210>  33
<211>  375
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FRB-20AA-mCherry

<400>  33

Met Thr Gly Ile Leu Trp His Glu Met Trp His Glu Gly Leu Glu Glu 
1               5                   10                  15      


Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe Glu 
            20                  25                  30          


Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln Thr Leu 
        35                  40                  45              


Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala 
    50                  55                  60                  


Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp Leu 
65                  70                  75                  80  


Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Lys 
                85                  90                  95      


Thr Gly Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
            100                 105                 110         


Gly Ser Gly Ser Gly Ser Thr Gly Val Ser Lys Gly Glu Glu Asp Asn 
        115                 120                 125             


Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys Val His Met Glu Gly 
    130                 135                 140                 


Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu Gly Glu Gly Arg 
145                 150                 155                 160 


Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys Val Thr Lys Gly Gly 
                165                 170                 175     


Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe Met Tyr Gly 
            180                 185                 190         


Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro Asp Tyr Leu Lys 
        195                 200                 205             


Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val Met Asn Phe Glu 
    210                 215                 220                 


Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser Leu Gln Asp Gly 
225                 230                 235                 240 


Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe Pro Ser Asp 
                245                 250                 255     


Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu Ala Ser Ser Glu 
            260                 265                 270         


Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys Gln Arg 
        275                 280                 285             


Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala Glu Val Lys Thr Thr 
    290                 295                 300                 


Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro Gly Ala Tyr Asn Val Asn 
305                 310                 315                 320 


Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr Thr Ile Val Glu 
                325                 330                 335     


Gln Tyr Glu Arg Ala Glu Gly Arg His Ser Thr Gly Gly Met Asp Glu 
            340                 345                 350         


Leu Tyr Lys Thr Gly Leu Glu Val Leu Phe Gln Gly Pro Thr Gly His 
        355                 360                 365             


His His His His His Thr Gly 
    370                 375 


<210>  34
<211>  390
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FKBP-20AA-mTFP1

<400>  34

Met Thr Gly Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
1               5                   10                  15      


Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
            20                  25                  30          


Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro 
        35                  40                  45              


Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
    50                  55                  60                  


Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
65                  70                  75                  80  


Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
                85                  90                  95      


His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Thr Gly 
            100                 105                 110         


Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
        115                 120                 125             


Gly Ser Gly Ser Thr Gly Val Ser Lys Gly Glu Glu Gly Val Ile Lys 
    130                 135                 140                 


Pro Asp Met Lys Ile Lys Leu Lys Met Glu Gly Asn Val Asn Gly His 
145                 150                 155                 160 


Ala Phe Val Ile Glu Gly Glu Gly Glu Gly Lys Pro Tyr Asp Gly Thr 
                165                 170                 175     


Asn Thr Ile Asn Leu Glu Val Lys Glu Gly Ala Pro Leu Pro Phe Ser 
            180                 185                 190         


Tyr Asp Ile Leu Thr Thr Ala Phe Ala Tyr Gly Asn Arg Ala Phe Thr 
        195                 200                 205             


Lys Tyr Pro Asp Asp Ile Pro Asn Tyr Phe Lys Gln Ser Phe Pro Glu 
    210                 215                 220                 


Gly Tyr Ser Trp Glu Arg Thr Met Thr Phe Glu Asp Lys Gly Ile Val 
225                 230                 235                 240 


Lys Val Lys Ser Asp Ile Ser Met Glu Glu Asp Ser Phe Ile Tyr Glu 
                245                 250                 255     


Ile His Leu Lys Gly Glu Asn Phe Pro Pro Asn Gly Pro Val Met Gln 
            260                 265                 270         


Lys Lys Thr Thr Gly Trp Asp Ala Ser Thr Glu Arg Met Tyr Val Arg 
        275                 280                 285             


Asp Gly Val Leu Lys Gly Asp Val Lys His Lys Leu Leu Leu Glu Gly 
    290                 295                 300                 


Gly Gly His His Arg Val Asp Phe Lys Thr Ile Tyr Arg Ala Lys Lys 
305                 310                 315                 320 


Ala Val Lys Leu Pro Asp Tyr His Phe Val Asp His Arg Ile Glu Ile 
                325                 330                 335     


Leu Asn His Asp Lys Asp Tyr Asn Lys Val Thr Val Tyr Glu Ser Ala 
            340                 345                 350         


Val Ala Arg Asn Ser Thr Asp Gly Met Asp Glu Leu Tyr Lys Ser Gly 
        355                 360                 365             


Thr Gly Leu Glu Val Leu Phe Gln Gly Pro Thr Gly Ser Gly His His 
    370                 375                 380                 


His His His His Thr Gly 
385                 390 


<210>  35
<211>  901
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCitrine-10-cRaf

<400>  35

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Phe Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Ser 
225                 230                 235                 240 


Gly Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Thr Gly Met Glu His 
                245                 250                 255     


Ile Gln Gly Ala Trp Lys Thr Ile Ser Asn Gly Phe Gly Phe Lys Asp 
            260                 265                 270         


Ala Val Phe Asp Gly Ser Ser Cys Ile Ser Pro Thr Ile Val Gln Gln 
        275                 280                 285             


Phe Gly Tyr Gln Arg Arg Ala Ser Asp Asp Gly Lys Leu Thr Asp Pro 
    290                 295                 300                 


Ser Lys Thr Ser Asn Thr Ile Arg Val Phe Leu Pro Asn Lys Gln Arg 
305                 310                 315                 320 


Thr Val Val Asn Val Arg Asn Gly Met Ser Leu His Asp Cys Leu Met 
                325                 330                 335     


Lys Ala Leu Lys Val Arg Gly Leu Gln Pro Glu Cys Cys Ala Val Phe 
            340                 345                 350         


Arg Leu Leu His Glu His Lys Gly Lys Lys Ala Arg Leu Asp Trp Asn 
        355                 360                 365             


Thr Asp Ala Ala Ser Leu Ile Gly Glu Glu Leu Gln Val Asp Phe Leu 
    370                 375                 380                 


Asp His Val Pro Leu Thr Thr His Asn Phe Ala Arg Lys Thr Phe Leu 
385                 390                 395                 400 


Lys Leu Ala Phe Cys Asp Ile Cys Gln Lys Phe Leu Leu Asn Gly Phe 
                405                 410                 415     


Arg Cys Gln Thr Cys Gly Tyr Lys Phe His Glu His Cys Ser Thr Lys 
            420                 425                 430         


Val Pro Thr Met Cys Val Asp Trp Ser Asn Ile Arg Gln Leu Leu Leu 
        435                 440                 445             


Phe Pro Asn Ser Thr Ile Gly Asp Ser Gly Val Pro Ala Leu Pro Ser 
    450                 455                 460                 


Leu Thr Met Arg Arg Met Arg Glu Ser Val Ser Arg Met Pro Val Ser 
465                 470                 475                 480 


Ser Gln His Arg Tyr Ser Thr Pro His Ala Phe Thr Phe Asn Thr Ser 
                485                 490                 495     


Ser Pro Ser Ser Glu Gly Ser Leu Ser Gln Arg Gln Arg Ser Thr Ser 
            500                 505                 510         


Thr Pro Asn Val His Met Val Ser Thr Thr Leu Pro Val Asp Ser Arg 
        515                 520                 525             


Met Ile Glu Asp Ala Ile Arg Ser His Ser Glu Ser Ala Ser Pro Ser 
    530                 535                 540                 


Ala Leu Ser Ser Ser Pro Asn Asn Leu Ser Pro Thr Gly Trp Ser Gln 
545                 550                 555                 560 


Pro Lys Thr Pro Val Pro Ala Gln Arg Glu Arg Ala Pro Val Ser Gly 
                565                 570                 575     


Thr Gln Glu Lys Asn Lys Ile Arg Pro Arg Gly Gln Arg Asp Ser Ser 
            580                 585                 590         


Tyr Tyr Trp Glu Ile Glu Ala Ser Glu Val Met Leu Ser Thr Arg Ile 
        595                 600                 605             


Gly Ser Gly Ser Phe Gly Thr Val Tyr Lys Gly Lys Trp His Gly Asp 
    610                 615                 620                 


Val Ala Val Lys Ile Leu Lys Val Val Asp Pro Thr Pro Glu Gln Phe 
625                 630                 635                 640 


Gln Ala Phe Arg Asn Glu Val Ala Val Leu Arg Lys Thr Arg His Val 
                645                 650                 655     


Asn Ile Leu Leu Phe Met Gly Tyr Met Thr Lys Asp Asn Leu Ala Ile 
            660                 665                 670         


Val Thr Gln Trp Cys Glu Gly Ser Ser Leu Tyr Lys His Leu His Val 
        675                 680                 685             


Gln Glu Thr Lys Phe Gln Met Phe Gln Leu Ile Asp Ile Ala Arg Gln 
    690                 695                 700                 


Thr Ala Gln Gly Met Asp Tyr Leu His Ala Lys Asn Ile Ile His Arg 
705                 710                 715                 720 


Asp Met Lys Ser Asn Asn Ile Phe Leu His Glu Gly Leu Thr Val Lys 
                725                 730                 735     


Ile Gly Asp Phe Gly Leu Ala Thr Val Lys Ser Arg Trp Ser Gly Ser 
            740                 745                 750         


Gln Gln Val Glu Gln Pro Thr Gly Ser Val Leu Trp Met Ala Pro Glu 
        755                 760                 765             


Val Ile Arg Met Gln Asp Asn Asn Pro Phe Ser Phe Gln Ser Asp Val 
    770                 775                 780                 


Tyr Ser Tyr Gly Ile Val Leu Tyr Glu Leu Met Thr Gly Glu Leu Pro 
785                 790                 795                 800 


Tyr Ser His Ile Asn Asn Arg Asp Gln Ile Ile Phe Met Val Gly Arg 
                805                 810                 815     


Gly Tyr Ala Ser Pro Asp Leu Ser Lys Leu Tyr Lys Asn Cys Pro Lys 
            820                 825                 830         


Ala Met Lys Arg Leu Val Ala Asp Cys Val Lys Lys Val Lys Glu Glu 
        835                 840                 845             


Arg Pro Leu Phe Pro Gln Ile Leu Ser Ser Ile Glu Leu Leu Gln His 
    850                 855                 860                 


Ser Leu Pro Lys Ile Asn Arg Ser Ala Ser Glu Pro Ser Leu His Arg 
865                 870                 875                 880 


Ala Ala His Thr Glu Asp Ile Asn Ala Cys Thr Leu Thr Thr Ser Pro 
                885                 890                 895     


Arg Leu Pro Val Phe 
            900     


<210>  36
<211>  949
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCitrine~WW-10-cRaf

<400>  36

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Phe Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Ser 
225                 230                 235                 240 


Gly Phe Glu Ile Pro Asp Asp Val Pro Leu Pro Ala Gly Trp Glu Met 
                245                 250                 255     


Ala Lys Thr Ser Ser Gly Gln Arg Tyr Phe Lys Asn His Ile Asp Gln 
            260                 265                 270         


Thr Thr Thr Trp Gln Asp Pro Arg Lys Ala Met Leu Ser Gln Met Thr 
        275                 280                 285             


Gly Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Thr Gly Met Glu His 
    290                 295                 300                 


Ile Gln Gly Ala Trp Lys Thr Ile Ser Asn Gly Phe Gly Phe Lys Asp 
305                 310                 315                 320 


Ala Val Phe Asp Gly Ser Ser Cys Ile Ser Pro Thr Ile Val Gln Gln 
                325                 330                 335     


Phe Gly Tyr Gln Arg Arg Ala Ser Asp Asp Gly Lys Leu Thr Asp Pro 
            340                 345                 350         


Ser Lys Thr Ser Asn Thr Ile Arg Val Phe Leu Pro Asn Lys Gln Arg 
        355                 360                 365             


Thr Val Val Asn Val Arg Asn Gly Met Ser Leu His Asp Cys Leu Met 
    370                 375                 380                 


Lys Ala Leu Lys Val Arg Gly Leu Gln Pro Glu Cys Cys Ala Val Phe 
385                 390                 395                 400 


Arg Leu Leu His Glu His Lys Gly Lys Lys Ala Arg Leu Asp Trp Asn 
                405                 410                 415     


Thr Asp Ala Ala Ser Leu Ile Gly Glu Glu Leu Gln Val Asp Phe Leu 
            420                 425                 430         


Asp His Val Pro Leu Thr Thr His Asn Phe Ala Arg Lys Thr Phe Leu 
        435                 440                 445             


Lys Leu Ala Phe Cys Asp Ile Cys Gln Lys Phe Leu Leu Asn Gly Phe 
    450                 455                 460                 


Arg Cys Gln Thr Cys Gly Tyr Lys Phe His Glu His Cys Ser Thr Lys 
465                 470                 475                 480 


Val Pro Thr Met Cys Val Asp Trp Ser Asn Ile Arg Gln Leu Leu Leu 
                485                 490                 495     


Phe Pro Asn Ser Thr Ile Gly Asp Ser Gly Val Pro Ala Leu Pro Ser 
            500                 505                 510         


Leu Thr Met Arg Arg Met Arg Glu Ser Val Ser Arg Met Pro Val Ser 
        515                 520                 525             


Ser Gln His Arg Tyr Ser Thr Pro His Ala Phe Thr Phe Asn Thr Ser 
    530                 535                 540                 


Ser Pro Ser Ser Glu Gly Ser Leu Ser Gln Arg Gln Arg Ser Thr Ser 
545                 550                 555                 560 


Thr Pro Asn Val His Met Val Ser Thr Thr Leu Pro Val Asp Ser Arg 
                565                 570                 575     


Met Ile Glu Asp Ala Ile Arg Ser His Ser Glu Ser Ala Ser Pro Ser 
            580                 585                 590         


Ala Leu Ser Ser Ser Pro Asn Asn Leu Ser Pro Thr Gly Trp Ser Gln 
        595                 600                 605             


Pro Lys Thr Pro Val Pro Ala Gln Arg Glu Arg Ala Pro Val Ser Gly 
    610                 615                 620                 


Thr Gln Glu Lys Asn Lys Ile Arg Pro Arg Gly Gln Arg Asp Ser Ser 
625                 630                 635                 640 


Tyr Tyr Trp Glu Ile Glu Ala Ser Glu Val Met Leu Ser Thr Arg Ile 
                645                 650                 655     


Gly Ser Gly Ser Phe Gly Thr Val Tyr Lys Gly Lys Trp His Gly Asp 
            660                 665                 670         


Val Ala Val Lys Ile Leu Lys Val Val Asp Pro Thr Pro Glu Gln Phe 
        675                 680                 685             


Gln Ala Phe Arg Asn Glu Val Ala Val Leu Arg Lys Thr Arg His Val 
    690                 695                 700                 


Asn Ile Leu Leu Phe Met Gly Tyr Met Thr Lys Asp Asn Leu Ala Ile 
705                 710                 715                 720 


Val Thr Gln Trp Cys Glu Gly Ser Ser Leu Tyr Lys His Leu His Val 
                725                 730                 735     


Gln Glu Thr Lys Phe Gln Met Phe Gln Leu Ile Asp Ile Ala Arg Gln 
            740                 745                 750         


Thr Ala Gln Gly Met Asp Tyr Leu His Ala Lys Asn Ile Ile His Arg 
        755                 760                 765             


Asp Met Lys Ser Asn Asn Ile Phe Leu His Glu Gly Leu Thr Val Lys 
    770                 775                 780                 


Ile Gly Asp Phe Gly Leu Ala Thr Val Lys Ser Arg Trp Ser Gly Ser 
785                 790                 795                 800 


Gln Gln Val Glu Gln Pro Thr Gly Ser Val Leu Trp Met Ala Pro Glu 
                805                 810                 815     


Val Ile Arg Met Gln Asp Asn Asn Pro Phe Ser Phe Gln Ser Asp Val 
            820                 825                 830         


Tyr Ser Tyr Gly Ile Val Leu Tyr Glu Leu Met Thr Gly Glu Leu Pro 
        835                 840                 845             


Tyr Ser His Ile Asn Asn Arg Asp Gln Ile Ile Phe Met Val Gly Arg 
    850                 855                 860                 


Gly Tyr Ala Ser Pro Asp Leu Ser Lys Leu Tyr Lys Asn Cys Pro Lys 
865                 870                 875                 880 


Ala Met Lys Arg Leu Val Ala Asp Cys Val Lys Lys Val Lys Glu Glu 
                885                 890                 895     


Arg Pro Leu Phe Pro Gln Ile Leu Ser Ser Ile Glu Leu Leu Gln His 
            900                 905                 910         


Ser Leu Pro Lys Ile Asn Arg Ser Ala Ser Glu Pro Ser Leu His Arg 
        915                 920                 925             


Ala Ala His Thr Glu Asp Ile Asn Ala Cys Thr Leu Thr Thr Ser Pro 
    930                 935                 940                 


Arg Leu Pro Val Phe 
945                 


<210>  37
<211>  328
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD-mCitrine

<400>  37

Met Ser Lys Thr Ser Asn Thr Ile Arg Val Phe Leu Pro Asn Lys Gln 
1               5                   10                  15      


Arg Thr Val Val Asn Val Arg Asn Gly Met Ser Leu His Asp Cys Leu 
            20                  25                  30          


Met Lys Ala Leu Lys Val Arg Gly Leu Gln Pro Glu Cys Cys Ala Val 
        35                  40                  45              


Phe Arg Leu Leu His Glu His Lys Gly Lys Lys Ala Arg Leu Asp Trp 
    50                  55                  60                  


Asn Thr Asp Ala Ala Ser Leu Ile Gly Glu Glu Leu Gln Val Asp Phe 
65                  70                  75                  80  


Leu Asp Thr Gly Gly Ser Gly Ser Thr Gly Val Ser Lys Gly Glu Glu 
                85                  90                  95      


Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val 
            100                 105                 110         


Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr 
        115                 120                 125             


Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro 
    130                 135                 140                 


Val Pro Trp Pro Thr Leu Val Thr Thr Phe Gly Tyr Gly Leu Met Cys 
145                 150                 155                 160 


Phe Ala Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser 
                165                 170                 175     


Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp 
            180                 185                 190         


Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr 
        195                 200                 205             


Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly 
    210                 215                 220                 


Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val 
225                 230                 235                 240 


Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys 
                245                 250                 255     


Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr 
            260                 265                 270         


Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn 
        275                 280                 285             


His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser Lys Asp Pro Asn Glu Lys 
    290                 295                 300                 


Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr 
305                 310                 315                 320 


Leu Gly Met Asp Glu Leu Tyr Lys 
                325             


<210>  38
<211>  481
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cherry.X-50-hRas

<400>  38

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe 
1               5                   10                  15      


Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe 
            20                  25                  30          


Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr 
        35                  40                  45              


Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp 
    50                  55                  60                  


Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His 
65                  70                  75                  80  


Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe 
                85                  90                  95      


Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val 
            100                 105                 110         


Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys 
        115                 120                 125             


Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 


Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly 
145                 150                 155                 160 


Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly 
                165                 170                 175     


His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val 
            180                 185                 190         


Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser 
        195                 200                 205             


His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly 
    210                 215                 220                 


Arg His Ser Thr Gly Gly Ser Gly Ser Gly Ser Gly Ser Gly Thr Gly 
225                 230                 235                 240 


Thr Gly Ser Ser Gly Ser Ser Gly Ser Ser Thr Ser Glu Gly Gln Glu 
                245                 250                 255     


Gly Thr Gln Thr Gly Ser Thr Ser Thr Ser Gly Thr Gly Thr Gln Ser 
            260                 265                 270         


Gly Thr Gly Gly Thr Gln Ser Gly Thr Glu Thr Ser Thr Gly Ser Gly 
        275                 280                 285             


Glu Thr Thr Gly Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly 
    290                 295                 300                 


Gly Val Gly Lys Ser Ala Leu Thr Ile Gln Leu Ile Gln Asn His Phe 
305                 310                 315                 320 


Val Asp Glu Tyr Asp Pro Thr Ile Glu Asp Ser Tyr Arg Lys Gln Val 
                325                 330                 335     


Val Ile Asp Gly Glu Thr Cys Leu Leu Asp Ile Leu Asp Thr Ala Gly 
            340                 345                 350         


Gln Glu Glu Tyr Ser Ala Met Arg Asp Gln Tyr Met Arg Thr Gly Glu 
        355                 360                 365             


Gly Phe Leu Cys Val Phe Ala Ile Asn Asn Thr Lys Ser Phe Glu Asp 
    370                 375                 380                 


Ile His Gln Tyr Arg Glu Gln Ile Lys Arg Val Lys Asp Ser Asp Asp 
385                 390                 395                 400 


Val Pro Met Val Leu Val Gly Asn Lys Cys Asp Leu Ala Ala Arg Thr 
                405                 410                 415     


Val Glu Ser Arg Gln Ala Gln Asp Leu Ala Arg Ser Tyr Gly Ile Pro 
            420                 425                 430         


Tyr Ile Glu Thr Ser Ala Lys Thr Arg Gln Gly Val Glu Asp Ala Phe 
        435                 440                 445             


Tyr Thr Leu Val Arg Glu Ile Arg Gln His Lys Leu Arg Lys Leu Asn 
    450                 455                 460                 


Pro Pro Asp Glu Ser Gly Pro Gly Cys Met Ser Cys Lys Cys Val Leu 
465                 470                 475                 480 


Ser 
    


<210>  39
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cherry.Wp2-50-hRas

<400>  39

Thr Pro Lys Leu Ala Ser Asp Thr Met Val Ser Lys Gly Glu Glu Asp 
1               5                   10                  15      


Asn Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys Val His Met Glu 
            20                  25                  30          


Gly Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu Gly Glu Gly 
        35                  40                  45              


Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys Val Thr Lys Gly 
    50                  55                  60                  


Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe Met Tyr 
65                  70                  75                  80  


Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro Asp Tyr Leu 
                85                  90                  95      


Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val Met Asn Phe 
            100                 105                 110         


Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser Leu Gln Asp 
        115                 120                 125             


Gly Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe Pro Ser 
    130                 135                 140                 


Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu Ala Ser Ser 
145                 150                 155                 160 


Glu Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys Gln 
                165                 170                 175     


Arg Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala Glu Val Lys Thr 
            180                 185                 190         


Thr Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro Gly Ala Tyr Asn Val 
        195                 200                 205             


Asn Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr Thr Ile Val 
    210                 215                 220                 


Glu Gln Tyr Glu Arg Ala Glu Gly Arg His Ser Thr Gly Gly Ser Gly 
225                 230                 235                 240 


Pro Leu Pro Pro Tyr Thr Thr Gly Thr Gly Ser Ser Gly Ser Ser Gly 
                245                 250                 255     


Ser Ser Thr Ser Glu Gly Gln Glu Gly Thr Gln Thr Gly Ser Thr Ser 
            260                 265                 270         


Thr Ser Gly Thr Gly Thr Gln Ser Gly Thr Gly Gly Thr Gln Ser Gly 
        275                 280                 285             


Thr Glu Thr Ser Thr Gly Ser Gly Glu Thr Thr Gly Met Thr Glu Tyr 
    290                 295                 300                 


Lys Leu Val Val Val Gly Ala Gly Gly Val Gly Lys Ser Ala Leu Thr 
305                 310                 315                 320 


Ile Gln Leu Ile Gln Asn His Phe Val Asp Glu Tyr Asp Pro Thr Ile 
                325                 330                 335     


Glu Asp Ser Tyr Arg Lys Gln Val Val Ile Asp Gly Glu Thr Cys Leu 
            340                 345                 350         


Leu Asp Ile Leu Asp Thr Ala Gly Gln Glu Glu Tyr Ser Ala Met Arg 
        355                 360                 365             


Asp Gln Tyr Met Arg Thr Gly Glu Gly Phe Leu Cys Val Phe Ala Ile 
    370                 375                 380                 


Asn Asn Thr Lys Ser Phe Glu Asp Ile His Gln Tyr Arg Glu Gln Ile 
385                 390                 395                 400 


Lys Arg Val Lys Asp Ser Asp Asp Val Pro Met Val Leu Val Gly Asn 
                405                 410                 415     


Lys Cys Asp Leu Ala Ala Arg Thr Val Glu Ser Arg Gln Ala Gln Asp 
            420                 425                 430         


Leu Ala Arg Ser Tyr Gly Ile Pro Tyr Ile Glu Thr Ser Ala Lys Thr 
        435                 440                 445             


Arg Gln Gly Val Glu Asp Ala Phe Tyr Thr Leu Val Arg Glu Ile Arg 
    450                 455                 460                 


Gln His Lys Leu Arg Lys Leu Asn Pro Pro Asp Glu Ser Gly Pro Gly 
465                 470                 475                 480 


Cys Met Ser Cys Lys Cys Val Leu Ser 
                485                 


<210>  40
<211>  425
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCherry-6aa-hRas

<400>  40

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe 
1               5                   10                  15      


Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe 
            20                  25                  30          


Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr 
        35                  40                  45              


Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp 
    50                  55                  60                  


Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His 
65                  70                  75                  80  


Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe 
                85                  90                  95      


Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val 
            100                 105                 110         


Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys 
        115                 120                 125             


Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 


Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly 
145                 150                 155                 160 


Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly 
                165                 170                 175     


His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val 
            180                 185                 190         


Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser 
        195                 200                 205             


His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly 
    210                 215                 220                 


Arg His Ser Thr Gly Gly Ser Gly Gly Ser Thr Gly Met Thr Glu Tyr 
225                 230                 235                 240 


Lys Leu Val Val Val Gly Ala Gly Gly Val Gly Lys Ser Ala Leu Thr 
                245                 250                 255     


Ile Gln Leu Ile Gln Asn His Phe Val Asp Glu Tyr Asp Pro Thr Ile 
            260                 265                 270         


Glu Asp Ser Tyr Arg Lys Gln Val Val Ile Asp Gly Glu Thr Cys Leu 
        275                 280                 285             


Leu Asp Ile Leu Asp Thr Ala Gly Gln Glu Glu Tyr Ser Ala Met Arg 
    290                 295                 300                 


Asp Gln Tyr Met Arg Thr Gly Glu Gly Phe Leu Cys Val Phe Ala Ile 
305                 310                 315                 320 


Asn Asn Thr Lys Ser Phe Glu Asp Ile His Gln Tyr Arg Glu Gln Ile 
                325                 330                 335     


Lys Arg Val Lys Asp Ser Asp Asp Val Pro Met Val Leu Val Gly Asn 
            340                 345                 350         


Lys Cys Asp Leu Ala Ala Arg Thr Val Glu Ser Arg Gln Ala Gln Asp 
        355                 360                 365             


Leu Ala Arg Ser Tyr Gly Ile Pro Tyr Ile Glu Thr Ser Ala Lys Thr 
    370                 375                 380                 


Arg Gln Gly Val Glu Asp Ala Phe Tyr Thr Leu Val Arg Glu Ile Arg 
385                 390                 395                 400 


Gln His Lys Leu Arg Lys Leu Asn Pro Pro Asp Glu Ser Gly Pro Gly 
                405                 410                 415     


Cys Met Ser Cys Lys Cys Val Leu Ser 
            420                 425 


<210>  41
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mcitrine

<400>  41
gtatcgaagg gagaggagtt gtttacgggc gtcgtgccaa ttcttgtgga gttggacggt       60

gacgtgaatg gtcacaaatt ctcggtcagc ggggagggag aaggagatgc cacttacgga      120

aagctgacat tgaagttcat ctgtaccacg ggtaaactcc ccgtaccgtg gcctacgctc      180

gtgacaacgt tcggttacgg ccttatgtgt ttcgcaagat atccggatca catgaaacag      240

cacgactttt tcaagtccgc catgccggag ggttacgtac aagagagaac gatctttttc      300

aaagatgacg gcaactacaa gactcgcgcg gaagtgaagt ttgagggaga tacccttgtg      360

aatagaattg agcttaaggg gatcgacttc aaagaggacg gtaacattct gggccacaaa      420

cttgaataca actataactc gcataatgtg tacattatgg cggataaaca gaagaatggg      480

atcaaagtga atttcaagat caggcataac atcgaggacg gatcagtgca gttggctgac      540

cattatcagc agaacacgcc catcggtgac ggtccggtgc tgcttccgga taaccactac      600

ctttcatatc agtcgaagct gtccaaagat cccaacgaaa agcgcgatca tatggtcctg      660

ctggagtttg tcaccgcggc aggaatcaca ttgggaatgg acgaattgta taag            714


<210>  42
<211>  705
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mcherry

<400>  42
gttagcaagg gtgaagaaga taacatggca atcatcaaag agtttatgcg tttcaaggtg       60

cacatggagg gtagcgttaa cggtcacgag ttcgaaattg aaggtgaggg tgagggccgt      120

ccgtacgaag gtacccaaac cgccaaactg aaggttacga agggtggccc gttgccgttt      180

gcgtgggata ttctgagccc gcagttcatg tatggtagca aggcgtatgt aaagcaccca      240

gcggacatcc cggactatct gaaactgtcg tttccggaag gtttcaaatg ggagcgcgtc      300

atgaatttcg aggatggcgg cgttgtcacc gtgacccagg atagctccct gcaagatggc      360

gagttcattt acaaggtcaa actgcgcggc accaatttcc cgtccgacgg tccggttatg      420

caaaagaaaa ccatgggctg ggaagctagc agcgaaagaa tgtatccgga agatggtgca      480

ctgaagggtg agatcaaaca acgtctgaag ctgaaggacg gcggtcatta cgacgccgag      540

gtgaaaacca cctataaggc gaaaaagccg gttcagctgc cgggtgcgta taacgtcaat      600

atcaaactgg atatcacgag ccacaatgag gactacacga ttgtggagca atacgaacgc      660

gcggaaggtc gtcattctac gggtggcatg gacgaactgt acaag                      705


<210>  43
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mcitrine

<400>  43
gtctccaaag gtgaagagct gttcacgggt gttgtgccga ttctggttga actggacggt       60

gacgtcaatg gccacaagtt tagcgtcagc ggtgagggcg agggtgatgc gacctacggt      120

aaactgacct tgaagtttat ctgcaccact ggcaaactgc cggtcccttg gccgaccctt      180

gttaccacgt tcggttacgg cctgatgtgc tttgcccgct atccagacca catgaaacaa      240

cacgactttt tcaaatctgc gatgccggaa ggctacgtac aagagcgtac cattttcttc      300

aaggacgatg gtaactacaa aacccgcgca gaggtcaaat tcgaaggtga taccttggtc      360

aaccgtattg agctgaaggg cattgacttc aaagaagatg gcaatatcct gggtcataag      420

ttggagtaca actataacag ccacaatgtt tacattatgg ccgacaaaca gaagaatggc      480

atcaaggtga atttcaaaat ccgtcataac atcgaggacg gttccgttca gctggcggat      540

cactatcagc agaatacccc gattggcgat ggcccggtgc tgctgccgga caaccattat      600

ctgagctatc agtccaaact gagcaaagat ccgaatgaaa agcgcgacca catggtgctg      660

ttggaatttg ttaccgcagc gggcatcacg ctcggtatgg atgagctgta caaa            714


<210>  44
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mTFP1

<400>  44
gtctccaaag gtgaagaggg cgtcatcaaa cctgacatga agattaagct gaaaatggaa       60

ggtaacgtta acggccacgc atttgtgatt gaaggcgagg gcgaaggcaa accgtatgac      120

ggtaccaata ccattaacct ggaagtcaaa gaaggcgcac cgttgccgtt tagctatgat      180

atcctgacca ccgcatttgc ctatggcaat cgcgcgttca ccaaataccc ggacgatatc      240

ccgaattact tcaaacagag ctttcctgag ggctactctt gggagcgcac catgaccttt      300

gaggacaaag gtatcgttaa ggtgaaaagc gacattagca tggaagagga cagcttcatt      360

tacgagattc acctgaaggg cgagaatttc ccgccgaacg gtccggtgat gcagaagaaa      420

accaccggct gggatgcgag caccgagcgt atgtacgtgc gtgatggtgt cttgaaaggc      480

gacgttaaac acaagctgtt gctggaaggt ggtggccatc accgtgtcga cttcaaaact      540

atctatcgcg cgaagaaagc cgttaaactg ccggattatc acttcgtgga tcaccgcatc      600

gagatcctga atcatgataa ggactacaat aaggtcaccg tttatgagag cgccgttgcg      660

cgtaacagca ccgatggcat ggacgagctg tacaag                                696


<210>  45
<211>  210
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SH3 domain

<400>  45
gatgacaatt tcatctacaa ggcaaaagca ttgtatccgt acgatgccga cgacgatgac       60

gcgtacgaga tcagctttga gcaaaacgag atcctgcaag ttagcgacat tgagggccgt      120

tggtggaaag cgcgtcgcgc caacggcgaa accggcatca ttccgagcaa ctatgtgcag      180

ctgattgatg gccctgaaga gatgcaccgc                                       210


<210>  46
<211>  138
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WW domain

<400>  46
ttcgagattc cagatgatgt tccgctgcca gcgggctggg aaatggcaaa aacgtccagc       60

ggtcaacgtt actttaagaa ccacatcgac caaaccacca cttggcagga cccgcgcaaa      120

gcgatgctga gccagatg                                                    138


<210>  47
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sp1

<400>  47
attccatcta aaccgctgcc accgctgcca gtt                                    33


<210>  48
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sp2

<400>  48
attgttaata aaccactggc tccactgccg gta                                    33


<210>  49
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wp1

<400>  49
ggtactccac caccgccata tactgttggc                                        30


<210>  50
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wp2

<400>  50
ccactgccac catatact                                                     18


<210>  51
<211>  930
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mcitrine - long spacer - SH3

<400>  51
gtctccaaag gtgaagagct gttcacgggt gttgtgccga ttctggttga actggacggt       60

gacgtcaatg gccacaagtt tagcgtcagc ggtgagggcg agggtgatgc gacctacggt      120

aaactgacct tgaagtttat ctgcaccact ggcaaactgc cggtcccttg gccgaccctt      180

gttaccacgt tcggttacgg cctgatgtgc tttgcccgct atccagacca catgaaacaa      240

cacgactttt tcaaatctgc gatgccggaa ggctacgtac aagagcgtac cattttcttc      300

aaggacgatg gtaactacaa aacccgcgca gaggtcaaat tcgaaggtga taccttggtc      360

aaccgtattg agctgaaggg cattgacttc aaagaagatg gcaatatcct gggtcataag      420

ttggagtaca actataacag ccacaatgtt tacattatgg ccgacaaaca gaagaatggc      480

atcaaggtga atttcaaaat ccgtcataac atcgaggacg gttccgttca gctggcggat      540

cactatcagc agaatacccc gattggcgat ggcccggtgc tgctgccgga caaccattat      600

ctgagctatc agtccaaact gagcaaagat ccgaatgaaa agcgcgacca catggtgctg      660

ttggaatttg ttaccgcagc gggcatcacg ctcggtatgg atgagctgta caaatcaggt      720

gatgacaatt tcatctacaa ggcaaaagca ttgtatccgt acgatgccga cgacgatgac      780

gcgtacgaga tcagctttga gcaaaacgag atcctgcaag ttagcgacat tgagggccgt      840

tggtggaaag cgcgtcgcgc caacggcgaa accggcatca ttccgagcaa ctatgtgcag      900

ctgattgatg gccctgaaga gatgcaccgc                                       930


<210>  52
<211>  912
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mcitrine - short spacer - SH3

<400>  52
gtctccaaag gtgaagagct gttcacgggt gttgtgccga ttctggttga actggacggt       60

gacgtcaatg gccacaagtt tagcgtcagc ggtgagggcg agggtgatgc gacctacggt      120

aaactgacct tgaagtttat ctgcaccact ggcaaactgc cggtcccttg gccgaccctt      180

gttaccacgt tcggttacgg cctgatgtgc tttgcccgct atccagacca catgaaacaa      240

cacgactttt tcaaatctgc gatgccggaa ggctacgtac aagagcgtac cattttcttc      300

aaggacgatg gtaactacaa aacccgcgca gaggtcaaat tcgaaggtga taccttggtc      360

aaccgtattg agctgaaggg cattgacttc aaagaagatg gcaatatcct gggtcataag      420

ttggagtaca actataacag ccacaatgtt tacattatgg ccgacaaaca gaagaatggc      480

atcaaggtga atttcaaaat ccgtcataac atcgaggacg gttccgttca gctggcggat      540

cactatcagc agaatacccc gattggcgat ggcccggtgc tgctgccgga caaccattat      600

ctgagctatc agtccaaact gagcaaagat ccgaatgaaa agcgcgacca catggtgctg      660

ttggaatttg ttaccgcagc gggcatcacg ctcggttcag gtgatgacaa tttcatctac      720

aaggcaaaag cattgtatcc gtacgatgcc gacgacgatg acgcgtacga gatcagcttt      780

gagcaaaacg agatcctgca agttagcgac attgagggcc gttggtggaa agcgcgtcgc      840

gccaacggcg aaaccggcat cattccgagc aactatgtgc agctgattga tggccctgaa      900

gagatgcacc gc                                                          912


<210>  53
<211>  744
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCherry - long spacer - strong peptide for SH3

<400>  53
gttagcaagg gtgaagaaga taacatggca atcatcaaag agtttatgcg tttcaaggtg       60

cacatggagg gtagcgttaa cggtcacgag ttcgaaattg aaggtgaggg tgagggccgt      120

ccgtacgaag gtacccaaac cgccaaactg aaggttacga agggtggccc gttgccgttt      180

gcgtgggata ttctgagccc gcagttcatg tatggtagca aggcgtatgt aaagcaccca      240

gcggacatcc cggactatct gaaactgtcg tttccggaag gtttcaaatg ggagcgcgtc      300

atgaatttcg aggatggcgg cgttgtcacc gtgacccagg atagctccct gcaagatggc      360

gagttcattt acaaggtcaa actgcgcggc accaatttcc cgtccgacgg tccggttatg      420

caaaagaaaa ccatgggctg ggaagctagc agcgaaagaa tgtatccgga agatggtgca      480

ctgaagggtg agatcaaaca acgtctgaag ctgaaggacg gcggtcatta cgacgccgag      540

gtgaaaacca cctataaggc gaaaaagccg gttcagctgc cgggtgcgta taacgtcaat      600

atcaaactgg atatcacgag ccacaatgag gactacacga ttgtggagca atacgaacgc      660

gcggaaggtc gtcattctac gggtggcatg gacgaactgt acaagggttc aattccatct      720

aaaccgctgc caccgctgcc agtt                                             744


<210>  54
<211>  744
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCherry - long spacer - weak peptide for SH3

<400>  54
gttagcaagg gtgaagaaga taacatggca atcatcaaag agtttatgcg tttcaaggtg       60

cacatggagg gtagcgttaa cggtcacgag ttcgaaattg aaggtgaggg tgagggccgt      120

ccgtacgaag gtacccaaac cgccaaactg aaggttacga agggtggccc gttgccgttt      180

gcgtgggata ttctgagccc gcagttcatg tatggtagca aggcgtatgt aaagcaccca      240

gcggacatcc cggactatct gaaactgtcg tttccggaag gtttcaaatg ggagcgcgtc      300

atgaatttcg aggatggcgg cgttgtcacc gtgacccagg atagctccct gcaagatggc      360

gagttcattt acaaggtcaa actgcgcggc accaatttcc cgtccgacgg tccggttatg      420

caaaagaaaa ccatgggctg ggaagctagc agcgaaagaa tgtatccgga agatggtgca      480

ctgaagggtg agatcaaaca acgtctgaag ctgaaggacg gcggtcatta cgacgccgag      540

gtgaaaacca cctataaggc gaaaaagccg gttcagctgc cgggtgcgta taacgtcaat      600

atcaaactgg atatcacgag ccacaatgag gactacacga ttgtggagca atacgaacgc      660

gcggaaggtc gtcattctac gggtggcatg gacgaactgt acaagggttc aattgttaat      720

aaaccactgg ctccactgcc ggta                                             744


<210>  55
<211>  726
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCherry - short spacer - strong peptide for SH3

<400>  55
gttagcaagg gtgaagaaga taacatggca atcatcaaag agtttatgcg tttcaaggtg       60

cacatggagg gtagcgttaa cggtcacgag ttcgaaattg aaggtgaggg tgagggccgt      120

ccgtacgaag gtacccaaac cgccaaactg aaggttacga agggtggccc gttgccgttt      180

gcgtgggata ttctgagccc gcagttcatg tatggtagca aggcgtatgt aaagcaccca      240

gcggacatcc cggactatct gaaactgtcg tttccggaag gtttcaaatg ggagcgcgtc      300

atgaatttcg aggatggcgg cgttgtcacc gtgacccagg atagctccct gcaagatggc      360

gagttcattt acaaggtcaa actgcgcggc accaatttcc cgtccgacgg tccggttatg      420

caaaagaaaa ccatgggctg ggaagctagc agcgaaagaa tgtatccgga agatggtgca      480

ctgaagggtg agatcaaaca acgtctgaag ctgaaggacg gcggtcatta cgacgccgag      540

gtgaaaacca cctataaggc gaaaaagccg gttcagctgc cgggtgcgta taacgtcaat      600

atcaaactgg atatcacgag ccacaatgag gactacacga ttgtggagca atacgaacgc      660

gcggaaggtc gtcattctac gggtggctca ggtattccat ctaaaccgct gccaccgctg      720

ccagtt                                                                 726


<210>  56
<211>  726
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCherry - short spacer - weak peptide for SH3

<400>  56
gttagcaagg gtgaagaaga taacatggca atcatcaaag agtttatgcg tttcaaggtg       60

cacatggagg gtagcgttaa cggtcacgag ttcgaaattg aaggtgaggg tgagggccgt      120

ccgtacgaag gtacccaaac cgccaaactg aaggttacga agggtggccc gttgccgttt      180

gcgtgggata ttctgagccc gcagttcatg tatggtagca aggcgtatgt aaagcaccca      240

gcggacatcc cggactatct gaaactgtcg tttccggaag gtttcaaatg ggagcgcgtc      300

atgaatttcg aggatggcgg cgttgtcacc gtgacccagg atagctccct gcaagatggc      360

gagttcattt acaaggtcaa actgcgcggc accaatttcc cgtccgacgg tccggttatg      420

caaaagaaaa ccatgggctg ggaagctagc agcgaaagaa tgtatccgga agatggtgca      480

ctgaagggtg agatcaaaca acgtctgaag ctgaaggacg gcggtcatta cgacgccgag      540

gtgaaaacca cctataaggc gaaaaagccg gttcagctgc cgggtgcgta taacgtcaat      600

atcaaactgg atatcacgag ccacaatgag gactacacga ttgtggagca atacgaacgc      660

gcggaaggtc gtcattctac gggtggctca ggtattgtta ataaaccact ggctccactg      720

ccggta                                                                 726


<210>  57
<211>  858
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mcitrine - long spacer - WW

<400>  57
gtctccaaag gtgaagagct gttcacgggt gttgtgccga ttctggttga actggacggt       60

gacgtcaatg gccacaagtt tagcgtcagc ggtgagggcg agggtgatgc gacctacggt      120

aaactgacct tgaagtttat ctgcaccact ggcaaactgc cggtcccttg gccgaccctt      180

gttaccacgt tcggttacgg cctgatgtgc tttgcccgct atccagacca catgaaacaa      240

cacgactttt tcaaatctgc gatgccggaa ggctacgtac aagagcgtac cattttcttc      300

aaggacgatg gtaactacaa aacccgcgca gaggtcaaat tcgaaggtga taccttggtc      360

aaccgtattg agctgaaggg cattgacttc aaagaagatg gcaatatcct gggtcataag      420

ttggagtaca actataacag ccacaatgtt tacattatgg ccgacaaaca gaagaatggc      480

atcaaggtga atttcaaaat ccgtcataac atcgaggacg gttccgttca gctggcggat      540

cactatcagc agaatacccc gattggcgat ggcccggtgc tgctgccgga caaccattat      600

ctgagctatc agtccaaact gagcaaagat ccgaatgaaa agcgcgacca catggtgctg      660

ttggaatttg ttaccgcagc gggcatcacg ctcggtatgg atgagctgta caaatcaggt      720

ttcgagattc cagatgatgt tccgctgcca gcgggctggg aaatggcaaa aacgtccagc      780

ggtcaacgtt actttaagaa ccacatcgac caaaccacca cttggcagga cccgcgcaaa      840

gcgatgctga gccagatg                                                    858


<210>  58
<211>  840
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mcitrine - short spacer - WW

<400>  58
gtctccaaag gtgaagagct gttcacgggt gttgtgccga ttctggttga actggacggt       60

gacgtcaatg gccacaagtt tagcgtcagc ggtgagggcg agggtgatgc gacctacggt      120

aaactgacct tgaagtttat ctgcaccact ggcaaactgc cggtcccttg gccgaccctt      180

gttaccacgt tcggttacgg cctgatgtgc tttgcccgct atccagacca catgaaacaa      240

cacgactttt tcaaatctgc gatgccggaa ggctacgtac aagagcgtac cattttcttc      300

aaggacgatg gtaactacaa aacccgcgca gaggtcaaat tcgaaggtga taccttggtc      360

aaccgtattg agctgaaggg cattgacttc aaagaagatg gcaatatcct gggtcataag      420

ttggagtaca actataacag ccacaatgtt tacattatgg ccgacaaaca gaagaatggc      480

atcaaggtga atttcaaaat ccgtcataac atcgaggacg gttccgttca gctggcggat      540

cactatcagc agaatacccc gattggcgat ggcccggtgc tgctgccgga caaccattat      600

ctgagctatc agtccaaact gagcaaagat ccgaatgaaa agcgcgacca catggtgctg      660

ttggaatttg ttaccgcagc gggcatcacg ctcggttcag gtttcgagat tccagatgat      720

gttccgctgc cagcgggctg ggaaatggca aaaacgtcca gcggtcaacg ttactttaag      780

aaccacatcg accaaaccac cacttggcag gacccgcgca aagcgatgct gagccagatg      840


<210>  59
<211>  741
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCherry - long spacer - strong peptide for WW

<400>  59
gttagcaagg gtgaagaaga taacatggca atcatcaaag agtttatgcg tttcaaggtg       60

cacatggagg gtagcgttaa cggtcacgag ttcgaaattg aaggtgaggg tgagggccgt      120

ccgtacgaag gtacccaaac cgccaaactg aaggttacga agggtggccc gttgccgttt      180

gcgtgggata ttctgagccc gcagttcatg tatggtagca aggcgtatgt aaagcaccca      240

gcggacatcc cggactatct gaaactgtcg tttccggaag gtttcaaatg ggagcgcgtc      300

atgaatttcg aggatggcgg cgttgtcacc gtgacccagg atagctccct gcaagatggc      360

gagttcattt acaaggtcaa actgcgcggc accaatttcc cgtccgacgg tccggttatg      420

caaaagaaaa ccatgggctg ggaagctagc agcgaaagaa tgtatccgga agatggtgca      480

ctgaagggtg agatcaaaca acgtctgaag ctgaaggacg gcggtcatta cgacgccgag      540

gtgaaaacca cctataaggc gaaaaagccg gttcagctgc cgggtgcgta taacgtcaat      600

atcaaactgg atatcacgag ccacaatgag gactacacga ttgtggagca atacgaacgc      660

gcggaaggtc gtcattctac gggtggcatg gacgaactgt acaagggttc aggtactcca      720

ccaccgccat atactgttgg c                                                741


<210>  60
<211>  729
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCherry - long spacer - weak peptide for WW

<400>  60
gttagcaagg gtgaagaaga taacatggca atcatcaaag agtttatgcg tttcaaggtg       60

cacatggagg gtagcgttaa cggtcacgag ttcgaaattg aaggtgaggg tgagggccgt      120

ccgtacgaag gtacccaaac cgccaaactg aaggttacga agggtggccc gttgccgttt      180

gcgtgggata ttctgagccc gcagttcatg tatggtagca aggcgtatgt aaagcaccca      240

gcggacatcc cggactatct gaaactgtcg tttccggaag gtttcaaatg ggagcgcgtc      300

atgaatttcg aggatggcgg cgttgtcacc gtgacccagg atagctccct gcaagatggc      360

gagttcattt acaaggtcaa actgcgcggc accaatttcc cgtccgacgg tccggttatg      420

caaaagaaaa ccatgggctg ggaagctagc agcgaaagaa tgtatccgga agatggtgca      480

ctgaagggtg agatcaaaca acgtctgaag ctgaaggacg gcggtcatta cgacgccgag      540

gtgaaaacca cctataaggc gaaaaagccg gttcagctgc cgggtgcgta taacgtcaat      600

atcaaactgg atatcacgag ccacaatgag gactacacga ttgtggagca atacgaacgc      660

gcggaaggtc gtcattctac gggtggcatg gacgaactgt acaagggttc accactgcca      720

ccatatact                                                              729


<210>  61
<211>  723
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCherry - short spacer - strong peptide for WW

<400>  61
gttagcaagg gtgaagaaga taacatggca atcatcaaag agtttatgcg tttcaaggtg       60

cacatggagg gtagcgttaa cggtcacgag ttcgaaattg aaggtgaggg tgagggccgt      120

ccgtacgaag gtacccaaac cgccaaactg aaggttacga agggtggccc gttgccgttt      180

gcgtgggata ttctgagccc gcagttcatg tatggtagca aggcgtatgt aaagcaccca      240

gcggacatcc cggactatct gaaactgtcg tttccggaag gtttcaaatg ggagcgcgtc      300

atgaatttcg aggatggcgg cgttgtcacc gtgacccagg atagctccct gcaagatggc      360

gagttcattt acaaggtcaa actgcgcggc accaatttcc cgtccgacgg tccggttatg      420

caaaagaaaa ccatgggctg ggaagctagc agcgaaagaa tgtatccgga agatggtgca      480

ctgaagggtg agatcaaaca acgtctgaag ctgaaggacg gcggtcatta cgacgccgag      540

gtgaaaacca cctataaggc gaaaaagccg gttcagctgc cgggtgcgta taacgtcaat      600

atcaaactgg atatcacgag ccacaatgag gactacacga ttgtggagca atacgaacgc      660

gcggaaggtc gtcattctac gggtggctca ggtggtactc caccaccgcc atatactgtt      720

ggc                                                                    723


<210>  62
<211>  711
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCherry - short spacer - weak peptide for WW

<400>  62
gttagcaagg gtgaagaaga taacatggca atcatcaaag agtttatgcg tttcaaggtg       60

cacatggagg gtagcgttaa cggtcacgag ttcgaaattg aaggtgaggg tgagggccgt      120

ccgtacgaag gtacccaaac cgccaaactg aaggttacga agggtggccc gttgccgttt      180

gcgtgggata ttctgagccc gcagttcatg tatggtagca aggcgtatgt aaagcaccca      240

gcggacatcc cggactatct gaaactgtcg tttccggaag gtttcaaatg ggagcgcgtc      300

atgaatttcg aggatggcgg cgttgtcacc gtgacccagg atagctccct gcaagatggc      360

gagttcattt acaaggtcaa actgcgcggc accaatttcc cgtccgacgg tccggttatg      420

caaaagaaaa ccatgggctg ggaagctagc agcgaaagaa tgtatccgga agatggtgca      480

ctgaagggtg agatcaaaca acgtctgaag ctgaaggacg gcggtcatta cgacgccgag      540

gtgaaaacca cctataaggc gaaaaagccg gttcagctgc cgggtgcgta taacgtcaat      600

atcaaactgg atatcacgag ccacaatgag gactacacga ttgtggagca atacgaacgc      660

gcggaaggtc gtcattctac gggtggctca ggtccactgc caccatatac t               711


<210>  63
<211>  840
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mTFP1 long spacer WW domain

<400>  63
gtctccaaag gtgaagaggg cgtcatcaaa cctgacatga agattaagct gaaaatggaa       60

ggtaacgtta acggccacgc atttgtgatt gaaggcgagg gcgaaggcaa accgtatgac      120

ggtaccaata ccattaacct ggaagtcaaa gaaggcgcac cgttgccgtt tagctatgat      180

atcctgacca ccgcatttgc ctatggcaat cgcgcgttca ccaaataccc ggacgatatc      240

ccgaattact tcaaacagag ctttcctgag ggctactctt gggagcgcac catgaccttt      300

gaggacaaag gtatcgttaa ggtgaaaagc gacattagca tggaagagga cagcttcatt      360

tacgagattc acctgaaggg cgagaatttc ccgccgaacg gtccggtgat gcagaagaaa      420

accaccggct gggatgcgag caccgagcgt atgtacgtgc gtgatggtgt cttgaaaggc      480

gacgttaaac acaagctgtt gctggaaggt ggtggccatc accgtgtcga cttcaaaact      540

atctatcgcg cgaagaaagc cgttaaactg ccggattatc acttcgtgga tcaccgcatc      600

gagatcctga atcatgataa ggactacaat aaggtcaccg tttatgagag cgccgttgcg      660

cgtaacagca ccgatggcat ggacgagctg tacaagtcag gtttcgagat tccagatgat      720

gttccgctgc cagcgggctg ggaaatggca aaaacgtcca gcggtcaacg ttactttaag      780

aaccacatcg accaaaccac cacttggcag gacccgcgca aagcgatgct gagccagatg      840


<210>  64
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FKBP

<400>  64
ggtgtgcaag ttgaaacgat tagcccaggt gatggtcgta ccttcccgaa acgtggtcag       60

acgtgtgtgg tgcattacac gggtatgctg gaggacggta agaagttcga cagcagccgt      120

gatcgtaaca agccgtttaa gtttatgctg ggtaagcaag aagtgattcg tggttgggaa      180

gaaggtgtgg ctcaaatgag cgtcggccag cgcgcgaagc tgaccatcag cccggattac      240

gcgtatggtg caaccggcca cccgggcatt atcccgccac atgctacgct ggttttcgac      300

gttgagctgc tgaagttaga g                                                321


<210>  65
<211>  279
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FRB

<400>  65
atcctctggc atgaaatgtg gcacgagggt ttagaagagg ccagccgtct gtatttcggc       60

gagcgcaacg tgaaaggcat gtttgaggtg ctggaacctc tgcacgctat gatggagcgt      120

ggcccgcaga ctttgaaaga aaccagcttt aaccaggcgt acggccgtga tctgatggag      180

gcacaggagt ggtgccgtaa gtacatgaaa agcggtaatg tcaaagacct gttgcaagca      240

tgggacctgt actaccacgt gtttcgtcgc attagcaaa                             279


<210>  66
<211>  1944
<212>  DNA
<213>  Homo sapiens

<400>  66
atggagcaca tacagggagc ttggaagacg atcagcaatg gttttggatt caaagatgcc       60

gtgtttgatg gctccagctg catctctcct acaatagttc agcagtttgg ctatcagcgc      120

cgggcatcag atgatggcaa actcacagat ccttctaaga caagcaacac tatccgtgtt      180

ttcttgccga acaagcaaag aacagtggtc aatgtgcgaa atggaatgag cttgcatgac      240

tgccttatga aagcactcaa ggtgaggggc ctgcaaccag agtgctgtgc agtgttcaga      300

cttctccacg aacacaaagg taaaaaagca cgcttagatt ggaatactga tgctgcgtct      360

ttgattggag aagaacttca agtagatttc ctggatcatg ttcccctcac aacacacaac      420

tttgctcgga agacgttcct gaagcttgcc ttctgtgaca tctgtcagaa attcctgctc      480

aatggatttc gatgtcagac ttgtggctac aaatttcatg agcactgtag caccaaagta      540

cctactatgt gtgtggactg gagtaacatc agacaactct tattgtttcc aaattccact      600

attggtgata gtggagtccc agcactacct tctttgacta tgcgtcgtat gcgagagtct      660

gtttccagga tgcctgttag ttctcagcac agatattcta cacctcacgc cttcaccttt      720

aacacctcca gtccctcatc tgaaggttcc ctctcccaga ggcagaggtc gacatccaca      780

cctaatgtcc acatggtcag caccaccctg cctgtggaca gcaggatgat tgaggatgca      840

attcgaagtc acagcgaatc agcctcacct tcagccctgt ccagtagccc caacaatctg      900

agcccaacag gctggtcaca gccgaaaacc cccgtgccag cacaaagaga gcgggcacca      960

gtatctggga cccaggagaa aaacaaaatt aggcctcgtg gacagagaga ttcaagctat     1020

tattgggaaa tagaagccag tgaagtgatg ctgtccactc ggattgggtc aggctctttt     1080

ggaactgttt ataagggtaa atggcacgga gatgttgcag taaagatcct aaaggttgtc     1140

gacccaaccc cagagcaatt ccaggccttc aggaatgagg tggctgttct gcgcaaaaca     1200

cggcatgtga acattctgct tttcatgggg tacatgacaa aggacaacct ggcaattgtg     1260

acccagtggt gcgagggcag cagcctctac aaacacctgc atgtccagga gaccaagttt     1320

cagatgttcc agctaattga cattgcccgg cagacggctc agggaatgga ctatttgcat     1380

gcaaagaaca tcatccatag agacatgaaa tccaacaata tatttctcca tgaaggctta     1440

acagtgaaaa ttggagattt tggtttggca acagtaaagt cacgctggag tggttctcag     1500

caggttgaac aacctactgg ctctgtcctc tggatggccc cagaggtgat ccgaatgcag     1560

gataacaacc cattcagttt ccagtcggat gtctactcct atggcatcgt attgtatgaa     1620

ctgatgacgg gggagcttcc ttattctcac atcaacaacc gagatcagat catcttcatg     1680

gtgggccgag gatatgcctc cccagatctt agtaagctat ataagaactg ccccaaagca     1740

atgaagaggc tggtagctga ctgtgtgaag aaagtaaagg aagagaggcc tctttttccc     1800

cagatcctgt cttccattga gctgctccaa cactctctac cgaagatcaa ccggagcgct     1860

tccgagccat ccttgcatcg ggcagcccac actgaggata tcaatgcttg cacgctgacc     1920

acgtccccga ggctgcctgt cttc                                            1944


<210>  67
<211>  243
<212>  DNA
<213>  Homo sapiens

<400>  67
tctaagacaa gcaacactat ccgtgttttc ttgccgaaca agcaaagaac agtggtcaat       60

gtgcgaaatg gaatgagctt gcatgactgc cttatgaaag cactcaaggt gaggggcctg      120

caaccagagt gctgtgcagt gttcagactt ctccacgaac acaaaggtaa aaaagcacgc      180

ttagattgga atactgatgc tgcgtctttg attggagaag aacttcaagt agatttcctg      240

gat                                                                    243


<210>  68
<211>  567
<212>  DNA
<213>  Homo sapiens

<400>  68
atgacggaat ataagctggt ggtggtgggc gcgggcggtg tgggcaagag tgcgctgacc       60

atccagctga tccagaacca ctttgtggac gaatacgacc ccactataga ggattcctac      120

cggaagcagg tggtcattga tggggagacg tgcctgttgg acatcctgga taccgcgggc      180

caggaggagt acagcgccat gcgggaccag tacatgcgca ccggggaggg cttcctgtgt      240

gtgtttgcca tcaacaacac caagtctttt gaggacatcc accagtacag ggagcagatc      300

aaacgggtga aggactcgga tgacgtgccc atggtgctgg tggggaacaa gtgtgacctg      360

gctgcacgca ctgtggaatc tcggcaggct caggacctcg cccgaagcta cggcatcccc      420

tacatcgaga cctcggccaa gacccggcag ggagtggagg atgccttcta cacgttggtg      480

cgtgagatcc ggcagcacaa gctgcggaag ctgaaccctc ctgatgagag tggccccggc      540

tgcatgagct gcaagtgtgt gctctcc                                          567


<210>  69
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  [GS]5 10aa linker

<400>  69

Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
1               5                   10  


<210>  70
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  [GS]10 20aa linker

<400>  70

Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
1               5                   10                  15      


Gly Ser Gly Ser 
            20  


<210>  71
<211>  50
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B50 linker sequence

<400>  71

Thr Gly Ser Ser Gly Ser Ser Gly Ser Ser Thr Ser Glu Gly Gln Glu 
1               5                   10                  15      


Gly Thr Gln Thr Gly Ser Thr Ser Thr Ser Gly Thr Gly Thr Gln Ser 
            20                  25                  30          


Gly Thr Gly Gly Thr Gln Ser Gly Thr Glu Thr Ser Thr Gly Ser Gly 
        35                  40                  45              


Glu Thr 
    50  


<210>  72
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Factor Xa cleavage site

<400>  72

Ile Glu Gly Arg 
1               


<210>  73
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  the thrombin cleavage site

<400>  73

Leu Val Pro Arg 
1               


<210>  74
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  the enterokinase cleaving site

<400>  74

Asp Asp Asp Asp Lys 
1               5   


<210>  75
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PreScission cleavage site

<400>  75

Leu Glu Val Leu Phe Gln Gly Pro 
1               5               


<210>  76
<211>  765
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  bRaf

<400>  76

Ala Ala Leu Ser Gly Gly Gly Gly Gly Gly Ala Glu Pro Gly Gln Ala 
1               5                   10                  15      


Leu Phe Asn Gly Asp Met Glu Pro Glu Ala Gly Ala Gly Ala Gly Ala 
            20                  25                  30          


Ala Ala Ser Ser Ala Ala Asp Pro Ala Ile Pro Glu Glu Val Trp Asn 
        35                  40                  45              


Ile Lys Gln Met Ile Lys Leu Thr Gln Glu His Ile Glu Ala Leu Leu 
    50                  55                  60                  


Asp Lys Phe Gly Gly Glu His Asn Pro Pro Ser Ile Tyr Leu Glu Ala 
65                  70                  75                  80  


Tyr Glu Glu Tyr Thr Ser Lys Leu Asp Ala Leu Gln Gln Arg Glu Gln 
                85                  90                  95      


Gln Leu Leu Glu Ser Leu Gly Asn Gly Thr Asp Phe Ser Val Ser Ser 
            100                 105                 110         


Ser Ala Ser Met Asp Thr Val Thr Ser Ser Ser Ser Ser Ser Leu Ser 
        115                 120                 125             


Val Leu Pro Ser Ser Leu Ser Val Phe Gln Asn Pro Thr Asp Val Ala 
    130                 135                 140                 


Arg Ser Asn Pro Lys Ser Pro Gln Lys Pro Ile Val Arg Val Phe Leu 
145                 150                 155                 160 


Pro Asn Lys Gln Arg Thr Val Val Pro Ala Arg Cys Gly Val Thr Val 
                165                 170                 175     


Arg Asp Ser Leu Lys Lys Ala Leu Met Met Arg Gly Leu Ile Pro Glu 
            180                 185                 190         


Cys Cys Ala Val Tyr Arg Ile Gln Asp Gly Glu Lys Lys Pro Ile Gly 
        195                 200                 205             


Trp Asp Thr Asp Ile Ser Trp Leu Thr Gly Glu Glu Leu His Val Glu 
    210                 215                 220                 


Val Leu Glu Asn Val Pro Leu Thr Thr His Asn Phe Val Arg Lys Thr 
225                 230                 235                 240 


Phe Phe Thr Leu Ala Phe Cys Asp Phe Cys Arg Lys Leu Leu Phe Gln 
                245                 250                 255     


Gly Phe Arg Cys Gln Thr Cys Gly Tyr Lys Phe His Gln Arg Cys Ser 
            260                 265                 270         


Thr Glu Val Pro Leu Met Cys Val Asn Tyr Asp Gln Leu Asp Leu Leu 
        275                 280                 285             


Phe Val Ser Lys Phe Phe Glu His His Pro Ile Pro Gln Glu Glu Ala 
    290                 295                 300                 


Ser Leu Ala Glu Thr Ala Leu Thr Ser Gly Ser Ser Pro Ser Ala Pro 
305                 310                 315                 320 


Ala Ser Asp Ser Ile Gly Pro Gln Ile Leu Thr Ser Pro Ser Pro Ser 
                325                 330                 335     


Lys Ser Ile Pro Ile Pro Gln Pro Phe Arg Pro Ala Asp Glu Asp His 
            340                 345                 350         


Arg Asn Gln Phe Gly Gln Arg Asp Arg Ser Ser Ser Ala Pro Asn Val 
        355                 360                 365             


His Ile Asn Thr Ile Glu Pro Val Asn Ile Asp Asp Leu Ile Arg Asp 
    370                 375                 380                 


Gln Gly Phe Arg Gly Asp Gly Gly Ser Thr Thr Gly Leu Ser Ala Thr 
385                 390                 395                 400 


Pro Pro Ala Ser Leu Pro Gly Ser Leu Thr Asn Val Lys Ala Leu Gln 
                405                 410                 415     


Lys Ser Pro Gly Pro Gln Arg Glu Arg Lys Ser Ser Ser Ser Ser Glu 
            420                 425                 430         


Asp Arg Asn Arg Met Lys Thr Leu Gly Arg Arg Asp Ser Ser Asp Asp 
        435                 440                 445             


Trp Glu Ile Pro Asp Gly Gln Ile Thr Val Gly Gln Arg Ile Gly Ser 
    450                 455                 460                 


Gly Ser Phe Gly Thr Val Tyr Lys Gly Lys Trp His Gly Asp Val Ala 
465                 470                 475                 480 


Val Lys Met Leu Asn Val Thr Ala Pro Thr Pro Gln Gln Leu Gln Ala 
                485                 490                 495     


Phe Lys Asn Glu Val Gly Val Leu Arg Lys Thr Arg His Val Asn Ile 
            500                 505                 510         


Leu Leu Phe Met Gly Tyr Ser Thr Lys Pro Gln Leu Ala Ile Val Thr 
        515                 520                 525             


Gln Trp Cys Glu Gly Ser Ser Leu Tyr His His Leu His Ile Ile Glu 
    530                 535                 540                 


Thr Lys Phe Glu Met Ile Lys Leu Ile Asp Ile Ala Arg Gln Thr Ala 
545                 550                 555                 560 


Gln Gly Met Asp Tyr Leu His Ala Lys Ser Ile Ile His Arg Asp Leu 
                565                 570                 575     


Lys Ser Asn Asn Ile Phe Leu His Glu Asp Leu Thr Val Lys Ile Gly 
            580                 585                 590         


Asp Phe Gly Leu Ala Thr Val Lys Ser Arg Trp Ser Gly Ser His Gln 
        595                 600                 605             


Phe Glu Gln Leu Ser Gly Ser Ile Leu Trp Met Ala Pro Glu Val Ile 
    610                 615                 620                 


Arg Met Gln Asp Lys Asn Pro Tyr Ser Phe Gln Ser Asp Val Tyr Ala 
625                 630                 635                 640 


Phe Gly Ile Val Leu Tyr Glu Leu Met Thr Gly Gln Leu Pro Tyr Ser 
                645                 650                 655     


Asn Ile Asn Asn Arg Asp Gln Ile Ile Phe Met Val Gly Arg Gly Tyr 
            660                 665                 670         


Leu Ser Pro Asp Leu Ser Lys Val Arg Ser Asn Cys Pro Lys Ala Met 
        675                 680                 685             


Lys Arg Leu Met Ala Glu Cys Leu Lys Lys Lys Arg Asp Glu Arg Pro 
    690                 695                 700                 


Leu Phe Pro Gln Ile Leu Ala Ser Ile Glu Leu Leu Ala Arg Ser Leu 
705                 710                 715                 720 


Pro Lys Ile His Arg Ser Ala Ser Glu Pro Ser Leu Asn Arg Ala Gly 
                725                 730                 735     


Phe Gln Thr Glu Asp Phe Ser Leu Tyr Ala Cys Ala Ser Pro Lys Thr 
            740                 745                 750         


Pro Ile Gln Ala Gly Gly Tyr Gly Ala Phe Pro Val His 
        755                 760                 765 


<210>  77
<211>  936
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rg2112

<400>  77

Met Glu His Ile Gln Gly Ala Trp Lys Thr Ile Ser Asn Gly Phe Gly 
1               5                   10                  15      


Phe Lys Asp Ala Val Phe Asp Gly Ser Ser Cys Ile Ser Pro Thr Ile 
            20                  25                  30          


Val Gln Gln Phe Gly Tyr Gln Arg Arg Ala Ser Asp Asp Gly Lys Leu 
        35                  40                  45              


Thr Asp Pro Ser Lys Thr Ser Asn Thr Ile Arg Val Phe Leu Pro Asn 
    50                  55                  60                  


Lys Gln Arg Thr Val Val Asn Val Arg Asn Gly Met Ser Leu His Asp 
65                  70                  75                  80  


Cys Leu Met Lys Ala Leu Lys Val Arg Gly Leu Gln Pro Glu Cys Cys 
                85                  90                  95      


Ala Val Phe Arg Leu Leu His Glu His Lys Gly Lys Lys Ala Arg Leu 
            100                 105                 110         


Asp Trp Asn Thr Asp Ala Ala Ser Leu Ile Gly Glu Glu Leu Gln Val 
        115                 120                 125             


Asp Phe Leu Asp His Val Pro Leu Thr Thr His Asn Phe Ala Arg Lys 
    130                 135                 140                 


Thr Phe Leu Lys Leu Ala Phe Cys Asp Ile Cys Gln Lys Phe Leu Leu 
145                 150                 155                 160 


Asn Gly Phe Arg Cys Gln Thr Cys Gly Tyr Lys Phe His Glu His Cys 
                165                 170                 175     


Ser Thr Lys Val Pro Thr Met Cys Val Asp Trp Ser Asn Ile Arg Gln 
            180                 185                 190         


Leu Leu Leu Phe Pro Asn Ser Thr Ile Gly Asp Ser Gly Val Pro Ala 
        195                 200                 205             


Leu Pro Ser Leu Thr Met Arg Arg Met Arg Glu Ser Val Ser Arg Met 
    210                 215                 220                 


Pro Val Ser Ser Gln His Arg Tyr Ser Thr Pro His Ala Phe Thr Phe 
225                 230                 235                 240 


Asn Thr Ser Ser Pro Ser Ser Glu Gly Ser Leu Ser Gln Arg Gln Arg 
                245                 250                 255     


Ser Thr Ser Thr Pro Asn Val His Met Val Ser Thr Thr Leu Pro Val 
            260                 265                 270         


Asp Ser Arg Met Ile Glu Asp Ala Ile Arg Ser His Ser Glu Ser Ala 
        275                 280                 285             


Ser Pro Ser Ala Leu Ser Ser Ser Pro Asn Asn Leu Ser Pro Thr Gly 
    290                 295                 300                 


Trp Ser Gln Pro Lys Thr Pro Val Pro Ala Gln Arg Glu Arg Ala Pro 
305                 310                 315                 320 


Val Ser Gly Thr Gln Glu Lys Asn Lys Ile Arg Pro Arg Gly Gln Arg 
                325                 330                 335     


Asp Ser Ser Tyr Tyr Trp Glu Ile Glu Ala Ser Glu Val Met Leu Ser 
            340                 345                 350         


Thr Arg Ile Gly Ser Gly Ser Phe Gly Thr Val Tyr Lys Gly Lys Trp 
        355                 360                 365             


His Gly Asp Val Ala Val Lys Ile Leu Lys Val Val Asp Pro Thr Pro 
    370                 375                 380                 


Glu Gln Phe Gln Ala Phe Arg Asn Glu Val Ala Val Leu Arg Lys Thr 
385                 390                 395                 400 


Arg His Val Asn Ile Leu Leu Phe Met Gly Tyr Met Thr Lys Asp Asn 
                405                 410                 415     


Leu Ala Ile Val Thr Gln Trp Cys Glu Gly Ser Ser Leu Tyr Lys His 
            420                 425                 430         


Leu His Val Gln Glu Thr Lys Phe Gln Met Phe Gln Leu Ile Asp Ile 
        435                 440                 445             


Ala Arg Gln Thr Ala Gln Gly Met Asp Tyr Leu His Ala Lys Asn Ile 
    450                 455                 460                 


Ile His Arg Asp Met Lys Ser Asn Asn Ile Phe Leu His Glu Gly Leu 
465                 470                 475                 480 


Thr Val Lys Ile Gly Asp Phe Gly Leu Ala Thr Val Lys Ser Arg Trp 
                485                 490                 495     


Ser Gly Ser Gln Gln Val Glu Gln Pro Thr Gly Ser Val Leu Trp Met 
            500                 505                 510         


Ala Pro Glu Val Ile Arg Met Gln Asp Asn Asn Pro Phe Ser Phe Gln 
        515                 520                 525             


Ser Asp Val Tyr Ser Tyr Gly Ile Val Leu Tyr Glu Leu Met Thr Gly 
    530                 535                 540                 


Glu Leu Pro Tyr Ser His Ile Asn Asn Arg Asp Gln Ile Ile Phe Met 
545                 550                 555                 560 


Val Gly Arg Gly Tyr Ala Ser Pro Asp Leu Ser Lys Leu Tyr Lys Asn 
                565                 570                 575     


Cys Pro Lys Ala Met Lys Arg Leu Val Ala Asp Cys Val Lys Lys Val 
            580                 585                 590         


Lys Glu Glu Arg Pro Leu Phe Pro Gln Ile Leu Ser Ser Ile Glu Leu 
        595                 600                 605             


Leu Gln His Ser Leu Pro Lys Ile Asn Arg Ser Ala Ser Glu Pro Ser 
    610                 615                 620                 


Leu His Arg Ala Ala His Thr Glu Asp Ile Asn Ala Cys Thr Leu Thr 
625                 630                 635                 640 


Thr Ser Pro Arg Leu Pro Val Phe Thr Gly Ser Ser Gln Thr Ser Gly 
                645                 650                 655     


Ser Ser Thr Ser Glu Gly Gln Glu Gly Thr Gln Thr Gly Ser Thr Ser 
            660                 665                 670         


Thr Ser Gly Thr Gly Thr Gln Ser Gly Thr Gly Gly Thr Gln Ser Gly 
        675                 680                 685             


Thr Glu Thr Ser Thr Gly Ser Gly Glu Thr Val Ser Lys Gly Glu Glu 
    690                 695                 700                 


Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val 
705                 710                 715                 720 


Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr 
                725                 730                 735     


Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro 
            740                 745                 750         


Val Pro Trp Pro Thr Leu Val Thr Thr Phe Gly Tyr Gly Leu Met Cys 
        755                 760                 765             


Phe Ala Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser 
    770                 775                 780                 


Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp 
785                 790                 795                 800 


Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr 
                805                 810                 815     


Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly 
            820                 825                 830         


Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val 
        835                 840                 845             


Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys 
    850                 855                 860                 


Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr 
865                 870                 875                 880 


Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn 
                885                 890                 895     


His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser Lys Asp Pro Asn Glu Lys 
            900                 905                 910         


Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr 
        915                 920                 925             


Leu Gly Met Asp Glu Leu Tyr Lys 
    930                 935     


<210>  78
<211>  984
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rg2113

<400>  78

Met Glu His Ile Gln Gly Ala Trp Lys Thr Ile Ser Asn Gly Phe Gly 
1               5                   10                  15      


Phe Lys Asp Ala Val Phe Asp Gly Ser Ser Cys Ile Ser Pro Thr Ile 
            20                  25                  30          


Val Gln Gln Phe Gly Tyr Gln Arg Arg Ala Ser Asp Asp Gly Lys Leu 
        35                  40                  45              


Thr Asp Pro Ser Lys Thr Ser Asn Thr Ile Arg Val Phe Leu Pro Asn 
    50                  55                  60                  


Lys Gln Arg Thr Val Val Asn Val Arg Asn Gly Met Ser Leu His Asp 
65                  70                  75                  80  


Cys Leu Met Lys Ala Leu Lys Val Arg Gly Leu Gln Pro Glu Cys Cys 
                85                  90                  95      


Ala Val Phe Arg Leu Leu His Glu His Lys Gly Lys Lys Ala Arg Leu 
            100                 105                 110         


Asp Trp Asn Thr Asp Ala Ala Ser Leu Ile Gly Glu Glu Leu Gln Val 
        115                 120                 125             


Asp Phe Leu Asp His Val Pro Leu Thr Thr His Asn Phe Ala Arg Lys 
    130                 135                 140                 


Thr Phe Leu Lys Leu Ala Phe Cys Asp Ile Cys Gln Lys Phe Leu Leu 
145                 150                 155                 160 


Asn Gly Phe Arg Cys Gln Thr Cys Gly Tyr Lys Phe His Glu His Cys 
                165                 170                 175     


Ser Thr Lys Val Pro Thr Met Cys Val Asp Trp Ser Asn Ile Arg Gln 
            180                 185                 190         


Leu Leu Leu Phe Pro Asn Ser Thr Ile Gly Asp Ser Gly Val Pro Ala 
        195                 200                 205             


Leu Pro Ser Leu Thr Met Arg Arg Met Arg Glu Ser Val Ser Arg Met 
    210                 215                 220                 


Pro Val Ser Ser Gln His Arg Tyr Ser Thr Pro His Ala Phe Thr Phe 
225                 230                 235                 240 


Asn Thr Ser Ser Pro Ser Ser Glu Gly Ser Leu Ser Gln Arg Gln Arg 
                245                 250                 255     


Ser Thr Ser Thr Pro Asn Val His Met Val Ser Thr Thr Leu Pro Val 
            260                 265                 270         


Asp Ser Arg Met Ile Glu Asp Ala Ile Arg Ser His Ser Glu Ser Ala 
        275                 280                 285             


Ser Pro Ser Ala Leu Ser Ser Ser Pro Asn Asn Leu Ser Pro Thr Gly 
    290                 295                 300                 


Trp Ser Gln Pro Lys Thr Pro Val Pro Ala Gln Arg Glu Arg Ala Pro 
305                 310                 315                 320 


Val Ser Gly Thr Gln Glu Lys Asn Lys Ile Arg Pro Arg Gly Gln Arg 
                325                 330                 335     


Asp Ser Ser Tyr Tyr Trp Glu Ile Glu Ala Ser Glu Val Met Leu Ser 
            340                 345                 350         


Thr Arg Ile Gly Ser Gly Ser Phe Gly Thr Val Tyr Lys Gly Lys Trp 
        355                 360                 365             


His Gly Asp Val Ala Val Lys Ile Leu Lys Val Val Asp Pro Thr Pro 
    370                 375                 380                 


Glu Gln Phe Gln Ala Phe Arg Asn Glu Val Ala Val Leu Arg Lys Thr 
385                 390                 395                 400 


Arg His Val Asn Ile Leu Leu Phe Met Gly Tyr Met Thr Lys Asp Asn 
                405                 410                 415     


Leu Ala Ile Val Thr Gln Trp Cys Glu Gly Ser Ser Leu Tyr Lys His 
            420                 425                 430         


Leu His Val Gln Glu Thr Lys Phe Gln Met Phe Gln Leu Ile Asp Ile 
        435                 440                 445             


Ala Arg Gln Thr Ala Gln Gly Met Asp Tyr Leu His Ala Lys Asn Ile 
    450                 455                 460                 


Ile His Arg Asp Met Lys Ser Asn Asn Ile Phe Leu His Glu Gly Leu 
465                 470                 475                 480 


Thr Val Lys Ile Gly Asp Phe Gly Leu Ala Thr Val Lys Ser Arg Trp 
                485                 490                 495     


Ser Gly Ser Gln Gln Val Glu Gln Pro Thr Gly Ser Val Leu Trp Met 
            500                 505                 510         


Ala Pro Glu Val Ile Arg Met Gln Asp Asn Asn Pro Phe Ser Phe Gln 
        515                 520                 525             


Ser Asp Val Tyr Ser Tyr Gly Ile Val Leu Tyr Glu Leu Met Thr Gly 
    530                 535                 540                 


Glu Leu Pro Tyr Ser His Ile Asn Asn Arg Asp Gln Ile Ile Phe Met 
545                 550                 555                 560 


Val Gly Arg Gly Tyr Ala Ser Pro Asp Leu Ser Lys Leu Tyr Lys Asn 
                565                 570                 575     


Cys Pro Lys Ala Met Lys Arg Leu Val Ala Asp Cys Val Lys Lys Val 
            580                 585                 590         


Lys Glu Glu Arg Pro Leu Phe Pro Gln Ile Leu Ser Ser Ile Glu Leu 
        595                 600                 605             


Leu Gln His Ser Leu Pro Lys Ile Asn Arg Ser Ala Ser Glu Pro Ser 
    610                 615                 620                 


Leu His Arg Ala Ala His Thr Glu Asp Ile Asn Ala Cys Thr Leu Thr 
625                 630                 635                 640 


Thr Ser Pro Arg Leu Pro Val Phe Thr Gly Ser Ser Gln Thr Ser Gly 
                645                 650                 655     


Ser Ser Thr Ser Glu Gly Gln Glu Gly Thr Gln Thr Gly Ser Thr Ser 
            660                 665                 670         


Thr Ser Gly Thr Gly Thr Gln Ser Gly Thr Gly Gly Thr Gln Ser Gly 
        675                 680                 685             


Thr Glu Thr Ser Thr Gly Ser Gly Glu Thr Val Ser Lys Gly Glu Glu 
    690                 695                 700                 


Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val 
705                 710                 715                 720 


Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr 
                725                 730                 735     


Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro 
            740                 745                 750         


Val Pro Trp Pro Thr Leu Val Thr Thr Phe Gly Tyr Gly Leu Met Cys 
        755                 760                 765             


Phe Ala Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser 
    770                 775                 780                 


Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp 
785                 790                 795                 800 


Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr 
                805                 810                 815     


Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly 
            820                 825                 830         


Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val 
        835                 840                 845             


Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys 
    850                 855                 860                 


Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr 
865                 870                 875                 880 


Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn 
                885                 890                 895     


His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser Lys Asp Pro Asn Glu Lys 
            900                 905                 910         


Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr 
        915                 920                 925             


Leu Gly Met Asp Glu Leu Tyr Lys Ser Gly Phe Glu Ile Pro Asp Asp 
    930                 935                 940                 


Val Pro Leu Pro Ala Gly Trp Glu Met Ala Lys Thr Ser Ser Gly Gln 
945                 950                 955                 960 


Arg Tyr Phe Lys Asn His Ile Asp Gln Thr Thr Thr Trp Gln Asp Pro 
                965                 970                 975     


Arg Lys Ala Met Leu Ser Gln Met 
            980                 


<210>  79
<211>  1055
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rg2116

<400>  79

Met Ala Ala Leu Ser Gly Gly Gly Gly Gly Gly Ala Glu Pro Gly Gln 
1               5                   10                  15      


Ala Leu Phe Asn Gly Asp Met Glu Pro Glu Ala Gly Ala Gly Ala Gly 
            20                  25                  30          


Ala Ala Ala Ser Ser Ala Ala Asp Pro Ala Ile Pro Glu Glu Val Trp 
        35                  40                  45              


Asn Ile Lys Gln Met Ile Lys Leu Thr Gln Glu His Ile Glu Ala Leu 
    50                  55                  60                  


Leu Asp Lys Phe Gly Gly Glu His Asn Pro Pro Ser Ile Tyr Leu Glu 
65                  70                  75                  80  


Ala Tyr Glu Glu Tyr Thr Ser Lys Leu Asp Ala Leu Gln Gln Arg Glu 
                85                  90                  95      


Gln Gln Leu Leu Glu Ser Leu Gly Asn Gly Thr Asp Phe Ser Val Ser 
            100                 105                 110         


Ser Ser Ala Ser Met Asp Thr Val Thr Ser Ser Ser Ser Ser Ser Leu 
        115                 120                 125             


Ser Val Leu Pro Ser Ser Leu Ser Val Phe Gln Asn Pro Thr Asp Val 
    130                 135                 140                 


Ala Arg Ser Asn Pro Lys Ser Pro Gln Lys Pro Ile Val Arg Val Phe 
145                 150                 155                 160 


Leu Pro Asn Lys Gln Arg Thr Val Val Pro Ala Arg Cys Gly Val Thr 
                165                 170                 175     


Val Arg Asp Ser Leu Lys Lys Ala Leu Met Met Arg Gly Leu Ile Pro 
            180                 185                 190         


Glu Cys Cys Ala Val Tyr Arg Ile Gln Asp Gly Glu Lys Lys Pro Ile 
        195                 200                 205             


Gly Trp Asp Thr Asp Ile Ser Trp Leu Thr Gly Glu Glu Leu His Val 
    210                 215                 220                 


Glu Val Leu Glu Asn Val Pro Leu Thr Thr His Asn Phe Val Arg Lys 
225                 230                 235                 240 


Thr Phe Phe Thr Leu Ala Phe Cys Asp Phe Cys Arg Lys Leu Leu Phe 
                245                 250                 255     


Gln Gly Phe Arg Cys Gln Thr Cys Gly Tyr Lys Phe His Gln Arg Cys 
            260                 265                 270         


Ser Thr Glu Val Pro Leu Met Cys Val Asn Tyr Asp Gln Leu Asp Leu 
        275                 280                 285             


Leu Phe Val Ser Lys Phe Phe Glu His His Pro Ile Pro Gln Glu Glu 
    290                 295                 300                 


Ala Ser Leu Ala Glu Thr Ala Leu Thr Ser Gly Ser Ser Pro Ser Ala 
305                 310                 315                 320 


Pro Ala Ser Asp Ser Ile Gly Pro Gln Ile Leu Thr Ser Pro Ser Pro 
                325                 330                 335     


Ser Lys Ser Ile Pro Ile Pro Gln Pro Phe Arg Pro Ala Asp Glu Asp 
            340                 345                 350         


His Arg Asn Gln Phe Gly Gln Arg Asp Arg Ser Ser Ser Ala Pro Asn 
        355                 360                 365             


Val His Ile Asn Thr Ile Glu Pro Val Asn Ile Asp Asp Leu Ile Arg 
    370                 375                 380                 


Asp Gln Gly Phe Arg Gly Asp Gly Gly Ser Thr Thr Gly Leu Ser Ala 
385                 390                 395                 400 


Thr Pro Pro Ala Ser Leu Pro Gly Ser Leu Thr Asn Val Lys Ala Leu 
                405                 410                 415     


Gln Lys Ser Pro Gly Pro Gln Arg Glu Arg Lys Ser Ser Ser Ser Ser 
            420                 425                 430         


Glu Asp Arg Asn Arg Met Lys Thr Leu Gly Arg Arg Asp Ser Ser Asp 
        435                 440                 445             


Asp Trp Glu Ile Pro Asp Gly Gln Ile Thr Val Gly Gln Arg Ile Gly 
    450                 455                 460                 


Ser Gly Ser Phe Gly Thr Val Tyr Lys Gly Lys Trp His Gly Asp Val 
465                 470                 475                 480 


Ala Val Lys Met Leu Asn Val Thr Ala Pro Thr Pro Gln Gln Leu Gln 
                485                 490                 495     


Ala Phe Lys Asn Glu Val Gly Val Leu Arg Lys Thr Arg His Val Asn 
            500                 505                 510         


Ile Leu Leu Phe Met Gly Tyr Ser Thr Lys Pro Gln Leu Ala Ile Val 
        515                 520                 525             


Thr Gln Trp Cys Glu Gly Ser Ser Leu Tyr His His Leu His Ile Ile 
    530                 535                 540                 


Glu Thr Lys Phe Glu Met Ile Lys Leu Ile Asp Ile Ala Arg Gln Thr 
545                 550                 555                 560 


Ala Gln Gly Met Asp Tyr Leu His Ala Lys Ser Ile Ile His Arg Asp 
                565                 570                 575     


Leu Lys Ser Asn Asn Ile Phe Leu His Glu Asp Leu Thr Val Lys Ile 
            580                 585                 590         


Gly Asp Phe Gly Leu Ala Thr Val Lys Ser Arg Trp Ser Gly Ser His 
        595                 600                 605             


Gln Phe Glu Gln Leu Ser Gly Ser Ile Leu Trp Met Ala Pro Glu Val 
    610                 615                 620                 


Ile Arg Met Gln Asp Lys Asn Pro Tyr Ser Phe Gln Ser Asp Val Tyr 
625                 630                 635                 640 


Ala Phe Gly Ile Val Leu Tyr Glu Leu Met Thr Gly Gln Leu Pro Tyr 
                645                 650                 655     


Ser Asn Ile Asn Asn Arg Asp Gln Ile Ile Phe Met Val Gly Arg Gly 
            660                 665                 670         


Tyr Leu Ser Pro Asp Leu Ser Lys Val Arg Ser Asn Cys Pro Lys Ala 
        675                 680                 685             


Met Lys Arg Leu Met Ala Glu Cys Leu Lys Lys Lys Arg Asp Glu Arg 
    690                 695                 700                 


Pro Leu Phe Pro Gln Ile Leu Ala Ser Ile Glu Leu Leu Ala Arg Ser 
705                 710                 715                 720 


Leu Pro Lys Ile His Arg Ser Ala Ser Glu Pro Ser Leu Asn Arg Ala 
                725                 730                 735     


Gly Phe Gln Thr Glu Asp Phe Ser Leu Tyr Ala Cys Ala Ser Pro Lys 
            740                 745                 750         


Thr Pro Ile Gln Ala Gly Gly Tyr Gly Ala Phe Pro Val His Thr Gly 
        755                 760                 765             


Ser Ser Gln Thr Ser Gly Ser Ser Thr Ser Glu Gly Gln Glu Gly Thr 
    770                 775                 780                 


Gln Thr Gly Ser Thr Ser Thr Ser Gly Thr Gly Thr Gln Ser Gly Thr 
785                 790                 795                 800 


Gly Gly Thr Gln Ser Gly Thr Glu Thr Ser Thr Gly Ser Gly Glu Thr 
                805                 810                 815     


Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met 
            820                 825                 830         


Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu 
        835                 840                 845             


Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala 
    850                 855                 860                 


Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile 
865                 870                 875                 880 


Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro 
                885                 890                 895     


Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys 
            900                 905                 910         


Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr 
        915                 920                 925             


Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu 
    930                 935                 940                 


Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr 
945                 950                 955                 960 


Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala 
                965                 970                 975     


Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His 
            980                 985                 990         


Tyr Asp Ala Glu Val Lys Thr Thr  Tyr Lys Ala Lys Lys  Pro Val Gln 
        995                 1000                 1005             


Leu Pro  Gly Ala Tyr Asn Val  Asn Ile Lys Leu Asp  Ile Thr Ser 
    1010                 1015                 1020             


His Asn  Glu Asp Tyr Thr Ile  Val Glu Gln Tyr Glu  Arg Ala Glu 
    1025                 1030                 1035             


Gly Arg  His Ser Thr Gly Gly  Ser Gly Ser Gly Ser  Gly Ser Gly 
    1040                 1045                 1050             


Thr Gly  
    1055 


<210>  80
<211>  1055
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rg2117

<400>  80

Met Ala Ala Leu Ser Gly Gly Gly Gly Gly Gly Ala Glu Pro Gly Gln 
1               5                   10                  15      


Ala Leu Phe Asn Gly Asp Met Glu Pro Glu Ala Gly Ala Gly Ala Gly 
            20                  25                  30          


Ala Ala Ala Ser Ser Ala Ala Asp Pro Ala Ile Pro Glu Glu Val Trp 
        35                  40                  45              


Asn Ile Lys Gln Met Ile Lys Leu Thr Gln Glu His Ile Glu Ala Leu 
    50                  55                  60                  


Leu Asp Lys Phe Gly Gly Glu His Asn Pro Pro Ser Ile Tyr Leu Glu 
65                  70                  75                  80  


Ala Tyr Glu Glu Tyr Thr Ser Lys Leu Asp Ala Leu Gln Gln Arg Glu 
                85                  90                  95      


Gln Gln Leu Leu Glu Ser Leu Gly Asn Gly Thr Asp Phe Ser Val Ser 
            100                 105                 110         


Ser Ser Ala Ser Met Asp Thr Val Thr Ser Ser Ser Ser Ser Ser Leu 
        115                 120                 125             


Ser Val Leu Pro Ser Ser Leu Ser Val Phe Gln Asn Pro Thr Asp Val 
    130                 135                 140                 


Ala Arg Ser Asn Pro Lys Ser Pro Gln Lys Pro Ile Val Arg Val Phe 
145                 150                 155                 160 


Leu Pro Asn Lys Gln Arg Thr Val Val Pro Ala Arg Cys Gly Val Thr 
                165                 170                 175     


Val Arg Asp Ser Leu Lys Lys Ala Leu Met Met Arg Gly Leu Ile Pro 
            180                 185                 190         


Glu Cys Cys Ala Val Tyr Arg Ile Gln Asp Gly Glu Lys Lys Pro Ile 
        195                 200                 205             


Gly Trp Asp Thr Asp Ile Ser Trp Leu Thr Gly Glu Glu Leu His Val 
    210                 215                 220                 


Glu Val Leu Glu Asn Val Pro Leu Thr Thr His Asn Phe Val Arg Lys 
225                 230                 235                 240 


Thr Phe Phe Thr Leu Ala Phe Cys Asp Phe Cys Arg Lys Leu Leu Phe 
                245                 250                 255     


Gln Gly Phe Arg Cys Gln Thr Cys Gly Tyr Lys Phe His Gln Arg Cys 
            260                 265                 270         


Ser Thr Glu Val Pro Leu Met Cys Val Asn Tyr Asp Gln Leu Asp Leu 
        275                 280                 285             


Leu Phe Val Ser Lys Phe Phe Glu His His Pro Ile Pro Gln Glu Glu 
    290                 295                 300                 


Ala Ser Leu Ala Glu Thr Ala Leu Thr Ser Gly Ser Ser Pro Ser Ala 
305                 310                 315                 320 


Pro Ala Ser Asp Ser Ile Gly Pro Gln Ile Leu Thr Ser Pro Ser Pro 
                325                 330                 335     


Ser Lys Ser Ile Pro Ile Pro Gln Pro Phe Arg Pro Ala Asp Glu Asp 
            340                 345                 350         


His Arg Asn Gln Phe Gly Gln Arg Asp Arg Ser Ser Ser Ala Pro Asn 
        355                 360                 365             


Val His Ile Asn Thr Ile Glu Pro Val Asn Ile Asp Asp Leu Ile Arg 
    370                 375                 380                 


Asp Gln Gly Phe Arg Gly Asp Gly Gly Ser Thr Thr Gly Leu Ser Ala 
385                 390                 395                 400 


Thr Pro Pro Ala Ser Leu Pro Gly Ser Leu Thr Asn Val Lys Ala Leu 
                405                 410                 415     


Gln Lys Ser Pro Gly Pro Gln Arg Glu Arg Lys Ser Ser Ser Ser Ser 
            420                 425                 430         


Glu Asp Arg Asn Arg Met Lys Thr Leu Gly Arg Arg Asp Ser Ser Asp 
        435                 440                 445             


Asp Trp Glu Ile Pro Asp Gly Gln Ile Thr Val Gly Gln Arg Ile Gly 
    450                 455                 460                 


Ser Gly Ser Phe Gly Thr Val Tyr Lys Gly Lys Trp His Gly Asp Val 
465                 470                 475                 480 


Ala Val Lys Met Leu Asn Val Thr Ala Pro Thr Pro Gln Gln Leu Gln 
                485                 490                 495     


Ala Phe Lys Asn Glu Val Gly Val Leu Arg Lys Thr Arg His Val Asn 
            500                 505                 510         


Ile Leu Leu Phe Met Gly Tyr Ser Thr Lys Pro Gln Leu Ala Ile Val 
        515                 520                 525             


Thr Gln Trp Cys Glu Gly Ser Ser Leu Tyr His His Leu His Ile Ile 
    530                 535                 540                 


Glu Thr Lys Phe Glu Met Ile Lys Leu Ile Asp Ile Ala Arg Gln Thr 
545                 550                 555                 560 


Ala Gln Gly Met Asp Tyr Leu His Ala Lys Ser Ile Ile His Arg Asp 
                565                 570                 575     


Leu Lys Ser Asn Asn Ile Phe Leu His Glu Asp Leu Thr Val Lys Ile 
            580                 585                 590         


Gly Asp Phe Gly Leu Ala Thr Val Lys Ser Arg Trp Ser Gly Ser His 
        595                 600                 605             


Gln Phe Glu Gln Leu Ser Gly Ser Ile Leu Trp Met Ala Pro Glu Val 
    610                 615                 620                 


Ile Arg Met Gln Asp Lys Asn Pro Tyr Ser Phe Gln Ser Asp Val Tyr 
625                 630                 635                 640 


Ala Phe Gly Ile Val Leu Tyr Glu Leu Met Thr Gly Gln Leu Pro Tyr 
                645                 650                 655     


Ser Asn Ile Asn Asn Arg Asp Gln Ile Ile Phe Met Val Gly Arg Gly 
            660                 665                 670         


Tyr Leu Ser Pro Asp Leu Ser Lys Val Arg Ser Asn Cys Pro Lys Ala 
        675                 680                 685             


Met Lys Arg Leu Met Ala Glu Cys Leu Lys Lys Lys Arg Asp Glu Arg 
    690                 695                 700                 


Pro Leu Phe Pro Gln Ile Leu Ala Ser Ile Glu Leu Leu Ala Arg Ser 
705                 710                 715                 720 


Leu Pro Lys Ile His Arg Ser Ala Ser Glu Pro Ser Leu Asn Arg Ala 
                725                 730                 735     


Gly Phe Gln Thr Glu Asp Phe Ser Leu Tyr Ala Cys Ala Ser Pro Lys 
            740                 745                 750         


Thr Pro Ile Gln Ala Gly Gly Tyr Gly Ala Phe Pro Val His Thr Gly 
        755                 760                 765             


Ser Ser Gln Thr Ser Gly Ser Ser Thr Ser Glu Gly Gln Glu Gly Thr 
    770                 775                 780                 


Gln Thr Gly Ser Thr Ser Thr Ser Gly Thr Gly Thr Gln Ser Gly Thr 
785                 790                 795                 800 


Gly Gly Thr Gln Ser Gly Thr Glu Thr Ser Thr Gly Ser Gly Glu Thr 
                805                 810                 815     


Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met 
            820                 825                 830         


Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu 
        835                 840                 845             


Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala 
    850                 855                 860                 


Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile 
865                 870                 875                 880 


Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro 
                885                 890                 895     


Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys 
            900                 905                 910         


Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr 
        915                 920                 925             


Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu 
    930                 935                 940                 


Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr 
945                 950                 955                 960 


Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala 
                965                 970                 975     


Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His 
            980                 985                 990         


Tyr Asp Ala Glu Val Lys Thr Thr  Tyr Lys Ala Lys Lys  Pro Val Gln 
        995                 1000                 1005             


Leu Pro  Gly Ala Tyr Asn Val  Asn Ile Lys Leu Asp  Ile Thr Ser 
    1010                 1015                 1020             


His Asn  Glu Asp Tyr Thr Ile  Val Glu Gln Tyr Glu  Arg Ala Glu 
    1025                 1030                 1035             


Gly Arg  His Ser Thr Gly Gly  Ser Gly Pro Leu Pro  Pro Tyr Thr 
    1040                 1045                 1050             


Thr Gly  
    1055 


<210>  81
<211>  2295
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  bRaf NT sequence

<400>  81
gcggcgctga gcggtggcgg tggtggcggc gcggagccgg gccaggctct gttcaacggg       60

gacatggagc ccgaggccgg cgccggcgcc ggcgccgcgg cctcttcggc tgcggaccct      120

gccattccgg aggaggtgtg gaatatcaaa caaatgatta agttgacaca ggaacatata      180

gaggccctat tggacaaatt tggtggggag cataatccac catcaatata tctggaggcc      240

tatgaagaat acaccagcaa gctagatgca ctccaacaaa gagaacaaca gttattggaa      300

tctctgggga acggaactga tttttctgtt tctagctctg catcaatgga taccgttaca      360

tcttcttcct cttctagcct ttcagtgcta ccttcatctc tttcagtttt tcaaaatccc      420

acagatgtgg cacggagcaa ccccaagtca ccacaaaaac ctatcgttag agtcttcctg      480

cccaacaaac agaggacagt ggtacctgca aggtgtggag ttacagtccg agacagtcta      540

aagaaagcac tgatgatgag aggtctaatc ccagagtgct gtgctgttta cagaattcag      600

gatggagaga agaaaccaat tggttgggac actgatattt cctggcttac tggagaagaa      660

ttgcatgtgg aagtgttgga gaatgttcca cttacaacac acaactttgt acgaaaaacg      720

tttttcacct tagcattttg tgacttttgt cgaaagctgc ttttccaggg tttccgctgt      780

caaacatgtg gttataaatt tcaccagcgt tgtagtacag aagttccact gatgtgtgtt      840

aattatgacc aacttgattt gctgtttgtc tccaagttct ttgaacacca cccaatacca      900

caggaagagg cgtccttagc agagactgcc ctaacatctg gatcatcccc ttccgcaccc      960

gcctcggact ctattgggcc ccaaattctc accagtccgt ctccttcaaa atccattcca     1020

attccacagc ccttccgacc agcagatgaa gatcatcgaa atcaatttgg gcaacgagac     1080

cgatcctcat cagctcccaa tgtgcatata aacacaatag aacctgtcaa tattgatgac     1140

ttgattagag accaaggatt tcgtggtgat ggaggatcaa ccacaggttt gtctgctacc     1200

ccccctgcct cattacctgg ctcactaact aacgtgaaag ccttacagaa atctccagga     1260

cctcagcgag aaaggaagtc atcttcatcc tcagaagaca ggaatcgaat gaaaacactt     1320

ggtagacggg actcgagtga tgattgggag attcctgatg ggcagattac agtgggacaa     1380

agaattggat ctggatcatt tggaacagtc tacaagggaa agtggcatgg tgatgtggca     1440

gtgaaaatgt tgaatgtgac agcacctaca cctcagcagt tacaagcctt caaaaatgaa     1500

gtaggagtac tcaggaaaac acgacatgtg aatatcctac tcttcatggg ctattccaca     1560

aagccacaac tggctattgt tacccagtgg tgtgagggct ccagcttgta tcaccatctc     1620

catatcattg agaccaaatt tgagatgatc aaacttatag atattgcacg acagactgca     1680

cagggcatgg attacttaca cgccaagtca atcatccaca gagacctcaa gagtaataat     1740

atatttcttc atgaagacct cacagtaaaa ataggtgatt ttggtctagc tacagtgaaa     1800

tctcgatgga gtgggtccca tcagtttgaa cagttgtctg gatccatttt gtggatggca     1860

ccagaagtca tcagaatgca agataaaaat ccatacagct ttcagtcaga tgtatatgca     1920

tttgggattg ttctgtatga attgatgact ggacagttac cttattcaaa catcaacaac     1980

agggaccaga taatttttat ggtgggacga ggatacctgt ctccagatct cagtaaggta     2040

cggagtaact gtccaaaagc catgaagaga ttaatggcag agtgcctcaa aaagaaaaga     2100

gatgagagac cactctttcc ccaaattctc gcctctattg agctgctggc ccgctcattg     2160

ccaaaaattc accgcagtgc atcagaaccc tccttgaatc gggctggttt ccaaacagag     2220

gattttagtc tatatgcttg tgcttctcca aaaacaccca tccaggcagg gggatatggt     2280

gcgtttcctg tccac                                                      2295


<210>  82
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mTurquoise2

<400>  82

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  


Ser Trp Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Phe Ser Asp Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  83
<211>  280
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  WW-mTurquoise2

<400>  83

Phe Glu Ile Pro Asp Asp Val Pro Leu Pro Ala Gly Trp Glu Met Ala 
1               5                   10                  15      


Lys Thr Ser Ser Gly Gln Arg Tyr Phe Lys Asn His Ile Asp Gln Thr 
            20                  25                  30          


Thr Thr Trp Gln Asp Pro Arg Lys Ala Met Val Ser Lys Gly Glu Glu 
        35                  40                  45              


Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val 
    50                  55                  60                  


Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr 
65                  70                  75                  80  


Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro 
                85                  90                  95      


Val Pro Trp Pro Thr Leu Val Thr Thr Leu Ser Trp Gly Val Gln Cys 
            100                 105                 110         


Phe Ala Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser 
        115                 120                 125             


Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp 
    130                 135                 140                 


Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr 
145                 150                 155                 160 


Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly 
                165                 170                 175     


Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Phe Ser Asp Asn Val 
            180                 185                 190         


Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys 
        195                 200                 205             


Ile Arg His Asn Ile Glu Asp Gly Gly Val Gln Leu Ala Asp His Tyr 
    210                 215                 220                 


Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn 
225                 230                 235                 240 


His Tyr Leu Ser Thr Gln Ser Lys Leu Ser Lys Asp Pro Asn Glu Lys 
                245                 250                 255     


Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr 
            260                 265                 270         


Leu Gly Met Asp Glu Leu Tyr Lys 
        275                 280 


<210>  84
<211>  237
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCitrine-Wp2

<400>  84

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ser Pro Leu Pro Pro Tyr Thr Thr Gly 
225                 230                 235         


<210>  85
<211>  286
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mTurquoise_Ww

<400>  85

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  


Ser Trp Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Phe Ser Asp Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly Ser 
                165                 170                 175     


Thr Gly Phe Glu Ile Pro Asp Asp Val Pro Leu Pro Ala Gly Trp Glu 
            180                 185                 190         


Met Ala Lys Thr Ser Ser Gly Gln Arg Tyr Phe Lys Asn His Ile Asp 
        195                 200                 205             


Gln Thr Thr Thr Trp Gln Asp Pro Arg Lys Ala Gly Gly Gly Gly Val 
    210                 215                 220                 


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
225                 230                 235                 240 


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu Ser 
                245                 250                 255     


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
            260                 265                 270         


Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
        275                 280                 285     


<210>  86
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCitrine_Xxinsertion

<400>  86

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Gly 
                165                 170                 175     


Ser Gly Ser Gly Thr Thr Gly Gly Ser Gly Ser Gln Ser Gly Ser Gly 
            180                 185                 190         


Thr Gly Ser Glu Ser Gly Ser Thr Gly Gly Ser Val Gln Leu Ala Asp 
        195                 200                 205             


His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro 
    210                 215                 220                 


Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser Lys Asp Pro Asn 
225                 230                 235                 240 


Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly 
                245                 250                 255     


Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
            260                 265     


<210>  87
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCitrine_Wp2insertion

<400>  87

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Pro 
                165                 170                 175     


Leu Pro Pro Tyr Thr Thr Gly Gly Ser Gly Ser Gln Ser Gly Ser Gly 
            180                 185                 190         


Thr Gly Ser Glu Ser Gly Ser Thr Gly Gly Ser Val Gln Leu Ala Asp 
        195                 200                 205             


His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro 
    210                 215                 220                 


Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser Lys Asp Pro Asn 
225                 230                 235                 240 


Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly 
                245                 250                 255     


Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
            260                 265     


<210>  88
<211>  485
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rg2130

<400>  88

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Ser Trp Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Phe Ser Asp Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Gly 
225                 230                 235                 240 


Gly Ser Gly Ser Leu Asp Glu Val Asp Gly Gly Ser Gly Ser Thr Gly 
                245                 250                 255     


Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
            260                 265                 270         


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
        275                 280                 285             


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
    290                 295                 300                 


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
305                 310                 315                 320 


Phe Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
                325                 330                 335     


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
            340                 345                 350         


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
        355                 360                 365             


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
    370                 375                 380                 


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
385                 390                 395                 400 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
                405                 410                 415     


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
            420                 425                 430         


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
        435                 440                 445             


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu 
    450                 455                 460                 


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
465                 470                 475                 480 


Val Thr Ala Ala Gly 
                485 


<210>  89
<211>  536
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rg2131

<400>  89

Met Phe Glu Ile Pro Asp Asp Val Pro Leu Pro Ala Gly Trp Glu Met 
1               5                   10                  15      


Ala Lys Thr Ser Ser Gly Gln Arg Tyr Phe Lys Asn His Ile Asp Gln 
            20                  25                  30          


Thr Thr Thr Trp Gln Asp Pro Arg Lys Ala Met Val Ser Lys Gly Glu 
        35                  40                  45              


Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp 
    50                  55                  60                  


Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala 
65                  70                  75                  80  


Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu 
                85                  90                  95      


Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Ser Trp Gly Val Gln 
            100                 105                 110         


Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys 
        115                 120                 125             


Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys 
    130                 135                 140                 


Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp 
145                 150                 155                 160 


Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp 
                165                 170                 175     


Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Phe Ser Asp Asn 
            180                 185                 190         


Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe 
        195                 200                 205             


Lys Ile Arg His Asn Ile Glu Asp Gly Gly Val Gln Leu Ala Asp His 
    210                 215                 220                 


Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp 
225                 230                 235                 240 


Asn His Tyr Leu Ser Thr Gln Ser Lys Leu Ser Lys Asp Pro Asn Glu 
                245                 250                 255     


Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile 
            260                 265                 270         


Thr Leu Gly Met Asp Glu Leu Tyr Lys Gly Gly Ser Gly Ser Leu Asp 
        275                 280                 285             


Glu Val Asp Gly Gly Ser Gly Ser Thr Gly Met Val Ser Lys Gly Glu 
    290                 295                 300                 


Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp 
305                 310                 315                 320 


Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala 
                325                 330                 335     


Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu 
            340                 345                 350         


Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe Gly Tyr Gly Leu Met 
        355                 360                 365             


Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys 
    370                 375                 380                 


Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys 
385                 390                 395                 400 


Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp 
                405                 410                 415     


Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp 
            420                 425                 430         


Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn 
        435                 440                 445             


Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe 
    450                 455                 460                 


Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His 
465                 470                 475                 480 


Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp 
                485                 490                 495     


Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser Lys Asp Pro Asn Glu 
            500                 505                 510         


Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ser 
        515                 520                 525             


Pro Leu Pro Pro Tyr Thr Thr Gly 
    530                 535     


<210>  90
<211>  571
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rg2132

<400>  90

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Ser Trp Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Phe Ser Asp Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly 
                165                 170                 175     


Ser Thr Gly Phe Glu Ile Pro Asp Asp Val Pro Leu Pro Ala Gly Trp 
            180                 185                 190         


Glu Met Ala Lys Thr Ser Ser Gly Gln Arg Tyr Phe Lys Asn His Ile 
        195                 200                 205             


Asp Gln Thr Thr Thr Trp Gln Asp Pro Arg Lys Ala Gly Gly Gly Gly 
    210                 215                 220                 


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
225                 230                 235                 240 


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu 
                245                 250                 255     


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
            260                 265                 270         


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Gly 
        275                 280                 285             


Gly Ser Gly Ser Leu Asp Glu Val Asp Gly Gly Ser Gly Ser Thr Gly 
    290                 295                 300                 


Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
305                 310                 315                 320 


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
                325                 330                 335     


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
            340                 345                 350         


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
        355                 360                 365             


Phe Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
    370                 375                 380                 


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
385                 390                 395                 400 


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
                405                 410                 415     


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
            420                 425                 430         


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
        435                 440                 445             


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
    450                 455                 460                 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
465                 470                 475                 480 


Gly Ser Gly Ser Gly Thr Thr Gly Gly Ser Gly Ser Gln Ser Gly Ser 
                485                 490                 495     


Gly Thr Gly Ser Glu Ser Gly Ser Thr Gly Gly Ser Val Gln Leu Ala 
            500                 505                 510         


Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu 
        515                 520                 525             


Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser Lys Asp Pro 
    530                 535                 540                 


Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala 
545                 550                 555                 560 


Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
                565                 570     


<210>  91
<211>  571
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rg2133

<400>  91

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Ser Trp Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Phe Ser Asp Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Gly 
                165                 170                 175     


Ser Thr Gly Phe Glu Ile Pro Asp Asp Val Pro Leu Pro Ala Gly Trp 
            180                 185                 190         


Glu Met Ala Lys Thr Ser Ser Gly Gln Arg Tyr Phe Lys Asn His Ile 
        195                 200                 205             


Asp Gln Thr Thr Thr Trp Gln Asp Pro Arg Lys Ala Gly Gly Gly Gly 
    210                 215                 220                 


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
225                 230                 235                 240 


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu 
                245                 250                 255     


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
            260                 265                 270         


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Gly 
        275                 280                 285             


Gly Ser Gly Ser Leu Asp Glu Val Asp Gly Gly Ser Gly Ser Thr Gly 
    290                 295                 300                 


Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
305                 310                 315                 320 


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
                325                 330                 335     


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
            340                 345                 350         


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
        355                 360                 365             


Phe Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
    370                 375                 380                 


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
385                 390                 395                 400 


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
                405                 410                 415     


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
            420                 425                 430         


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
        435                 440                 445             


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
    450                 455                 460                 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
465                 470                 475                 480 


Pro Leu Pro Pro Tyr Thr Thr Gly Gly Ser Gly Ser Gln Ser Gly Ser 
                485                 490                 495     


Gly Thr Gly Ser Glu Ser Gly Ser Thr Gly Gly Ser Val Gln Leu Ala 
            500                 505                 510         


Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu 
        515                 520                 525             


Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser Lys Asp Pro 
    530                 535                 540                 


Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala 
545                 550                 555                 560 


Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
                565                 570     


<210>  92
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mTurquoise2

<400>  92
gtgtcgaagg gggaagagtt gtttacggga gtagtgccga ttctcgtaga gcttgatggg       60

gacgtcaatg gtcacaagtt ttcggtatcc ggggaggggg agggtgacgc aacatacgga      120

aaactgacgt tgaagtttat ctgtacgacg ggaaaacttc cggtgccgtg gcccactttg      180

gtaacaactc tctcatgggg cgtgcagtgc ttcgcacgct atcccgatca catgaagcaa      240

catgactttt tcaagagcgc gatgccggag gggtacgtgc aagagagaac catcttcttc      300

aaggacgacg gaaactacaa aacacgggcg gaagtgaagt tcgaaggaga cacactggta      360

aacaggatcg aactgaaggg gattgacttt aaggaagatg gtaacatcct tggacataag      420

ctggagtata actacttttc cgataatgtc tatatcacgg cggataagca gaaaaacgga      480

atcaaggcca actttaagat tcgacataac attgaggatg gtggggtaca gctggcggac      540

cactaccagc aaaacacccc cattggggac gggcctgtgc tccttcccga caatcattat      600

ctctccactc agtcaaagct ctcgaaagat cccaatgaga aacgggacca tatggtcttg      660

ctggagttcg tcactgccgc aggcatcaca ctggggatgg acgagcttta caag            714


<210>  93
<211>  840
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WW-mTurquoise2

<400>  93
tttgagattc ccgacgatgt acctctccca gcgggatggg agatggcgaa aacctcgtcg       60

ggacaacgat actttaagaa ccatatcgat caaacgacaa cctggcagga cccccgcaaa      120

gccatggtgt cgaaggggga agagttgttt acgggagtag tgccgattct cgtagagctt      180

gatggggacg tcaatggtca caagttttcg gtatccgggg agggggaggg tgacgcaaca      240

tacggaaaac tgacgttgaa gtttatctgt acgacgggaa aacttccggt gccgtggccc      300

actttggtaa caactctctc atggggcgtg cagtgcttcg cacgctatcc cgatcacatg      360

aagcaacatg actttttcaa gagcgcgatg ccggaggggt acgtgcaaga gagaaccatc      420

ttcttcaagg acgacggaaa ctacaaaaca cgggcggaag tgaagttcga aggagacaca      480

ctggtaaaca ggatcgaact gaaggggatt gactttaagg aagatggtaa catccttgga      540

cataagctgg agtataacta cttttccgat aatgtctata tcacggcgga taagcagaaa      600

aacggaatca aggccaactt taagattcga cataacattg aggatggtgg ggtacagctg      660

gcggaccact accagcaaaa cacccccatt ggggacgggc ctgtgctcct tcccgacaat      720

cattatctct ccactcagtc aaagctctcg aaagatccca atgagaaacg ggaccatatg      780

gtcttgctgg agttcgtcac tgccgcaggc atcacactgg ggatggacga gctttacaag      840


<210>  94
<211>  711
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCitrine-Wp2

<400>  94
gtatcgaaag gtgaagagct gtttactggc gtcgtgccca ttttggtgga acttgacggt       60

gacgtgaacg gtcacaaatt ctcagtctcg ggagaaggcg agggggacgc gacatatggg      120

aaactcacac tgaagttcat ctgcacgact ggaaagctgc ctgtcccttg gcctacactc      180

gtcacgacgt tcggttatgg tttgatgtgt ttcgcaagat atcctgacca catgaaacag      240

catgatttct ttaagagcgc catgcccgaa ggttatgtcc aggagaggac tatcttcttt      300

aaggacgatg ggaactacaa gactcgggca gaggtgaaat ttgaggggga cacgcttgtg      360

aatagaatcg aactcaaagg catcgatttc aaggaggatg gaaacattct cggacacaaa      420

cttgagtaca attacaacag ccacaacgtg tatatcatgg cggacaaaca gaagaatgga      480

atcaaagtga acttcaagat cagacacaat atcgaagatg ggtcagtgca gctggctgat      540

cattaccagc agaatacgcc catcggggac ggtccagtct tgttgccgga taaccactac      600

ctttcatacc agtcgaagtt gtccaaagac cccaacgaga agagggatca catggtgctt      660

cttgaatttg tcacagccgc tggtagccct ttgccgccgt acacgacagg a               711


<210>  95
<211>  858
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mTurquoise_WWinsertion

<400>  95
gtgtcgaagg gggaagagtt gtttacggga gtagtgccga ttctcgtaga gcttgatggg       60

gacgtcaatg gtcacaagtt ttcggtatcc ggggaggggg agggtgacgc aacatacgga      120

aaactgacgt tgaagtttat ctgtacgacg ggaaaacttc cggtgccgtg gcccactttg      180

gtaacaactc tctcatgggg cgtgcagtgc ttcgcacgct atcccgatca catgaagcaa      240

catgactttt tcaagagcgc gatgccggag gggtacgtgc aagagagaac catcttcttc      300

aaggacgacg gaaactacaa aacacgggcg gaagtgaagt tcgaaggaga cacactggta      360

aacaggatcg aactgaaggg gattgacttt aaggaagatg gtaacatcct tggacataag      420

ctggagtata actacttttc cgataatgtc tatatcacgg cggataagca gaaaaacgga      480

atcaaggcca actttaagat tcgacataac attgaggatg gtgggtcgac agggttcgag      540

attccagatg atgtcccgct cccggctggg tgggaaatgg ccaaaacgtc cagcggacag      600

cgctatttca aaaatcacat cgaccaaacc accacgtggc aggacccgcg gaaggcggga      660

ggagggggtg tacagctggc ggaccactac cagcaaaaca cccccattgg ggacgggcct      720

gtgctccttc ccgacaatca ttatctctcc actcagtcaa agctctcgaa agatcccaat      780

gagaaacggg accatatggt cttgctggag ttcgtcactg ccgcaggcat cacactgggg      840

atggacgagc tttacaag                                                    858


<210>  96
<211>  798
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCitrine_XXinsertion

<400>  96
gtatcgaaag gtgaagagct gtttactggc gtcgtgccca ttttggtgga acttgacggt       60

gacgtgaacg gtcacaaatt ctcagtctcg ggagaaggcg agggggacgc gacatatggg      120

aaactcacac tgaagttcat ctgcacgact ggaaagctgc ctgtcccttg gcctacactc      180

gtcacgacgt tcggttatgg tttgatgtgt ttcgcaagat atcctgacca catgaaacag      240

catgatttct ttaagagcgc catgcccgaa ggttatgtcc aggagaggac tatcttcttt      300

aaggacgatg ggaactacaa gactcgggca gaggtgaaat ttgaggggga cacgcttgtg      360

aatagaatcg aactcaaagg catcgatttc aaggaggatg gaaacattct cggacacaaa      420

cttgagtaca attacaacag ccacaacgtg tatatcatgg cggacaaaca gaagaatgga      480

atcaaagtga acttcaagat cagacacaat atcgaagatg ggtcaggaag cggaagcggg      540

acgacaggag ggtccggttc ccagtcagga tcagggacgg gaagcgaatc ggggtccacc      600

ggaggaagcg tgcagctggc tgatcattac cagcagaata cgcccatcgg ggacggtcca      660

gtcttgttgc cggataacca ctacctttca taccagtcga agttgtccaa agaccccaac      720

gagaagaggg atcacatggt gcttcttgaa tttgtcacag ccgctggtat taccctcggt      780

atggatgaat tgtacaaa                                                    798


<210>  97
<211>  798
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCitrine_Wp2insertion

<400>  97
gtatcgaaag gtgaagagct gtttactggc gtcgtgccca ttttggtgga acttgacggt       60

gacgtgaacg gtcacaaatt ctcagtctcg ggagaaggcg agggggacgc gacatatggg      120

aaactcacac tgaagttcat ctgcacgact ggaaagctgc ctgtcccttg gcctacactc      180

gtcacgacgt tcggttatgg tttgatgtgt ttcgcaagat atcctgacca catgaaacag      240

catgatttct ttaagagcgc catgcccgaa ggttatgtcc aggagaggac tatcttcttt      300

aaggacgatg ggaactacaa gactcgggca gaggtgaaat ttgaggggga cacgcttgtg      360

aatagaatcg aactcaaagg catcgatttc aaggaggatg gaaacattct cggacacaaa      420

cttgagtaca attacaacag ccacaacgtg tatatcatgg cggacaaaca gaagaatgga      480

atcaaagtga acttcaagat cagacacaat atcgaagatg ggtcaccgtt gccaccctat      540

acgacaggag ggtccggttc ccagtcagga tcagggacgg gaagcgaatc ggggtccacc      600

ggaggaagcg tgcagctggc tgatcattac cagcagaata cgcccatcgg ggacggtcca      660

gtcttgttgc cggataacca ctacctttca taccagtcga agttgtccaa agaccccaac      720

gagaagaggg atcacatggt gcttcttgaa tttgtcacag ccgctggtat taccctcggt      780

atggatgaat tgtacaaa                                                    798


<210>  98
<211>  50
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C50 Linker sequence

<400>  98

Thr Gly Ser Ser Gln Thr Ser Gly Ser Ser Thr Ser Glu Gly Gln Glu 
1               5                   10                  15      


Gly Thr Gln Thr Gly Ser Thr Ser Thr Ser Gly Thr Gly Thr Gln Ser 
            20                  25                  30          


Gly Thr Gly Gly Thr Gln Ser Gly Thr Glu Thr Ser Thr Gly Ser Gly 
        35                  40                  45              


Glu Thr 
    50  


