                         SEQUENCE LISTING

<110>  FUNDACI PRIVADA CENTRE DE REGULACI GENMICA (CRG)
       INSTITUCI CATALANA DE RECERCA I ESTUDIS AVANATS (ICREA)
 
<120>  ENGINEERED FLUORESCENT PROTEINS FOR ENHANCED FRET AND USES 
       THEREOF

<130>  BAR/FMUT/397

<150>  GB 1121565.4
<151>  2011-12-15

<160>  37    

<170>  PatentIn version 3.5

<210>  1
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (m)Citrine


<220>
<221>  MISC_FEATURE
<222>  (206)..(206)
<223>  Xaa can be Ala or Lys

<400>  1

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Xaa Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  2
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Citrine

<400>  2

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  3
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCitrine

<400>  3

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Lys Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  4
<211>  235
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCherry

<400>  4

Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met 
1               5                   10                  15      


Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu 
            20                  25                  30          


Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala 
        35                  40                  45              


Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile 
    50                  55                  60                  


Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro 
65                  70                  75                  80  


Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys 
                85                  90                  95      


Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr 
            100                 105                 110         


Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu 
        115                 120                 125             


Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr 
    130                 135                 140                 


Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala 
145                 150                 155                 160 


Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His 
                165                 170                 175     


Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln 
            180                 185                 190         


Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser His 
        195                 200                 205             


Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg 
    210                 215                 220                 


His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235 


<210>  5
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCitrine 4-


<220>
<221>  MISC_FEATURE
<222>  (206)..(206)
<223>  Xaa can be Ala or Lys

<400>  5

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Glu Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asp 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Glu Tyr Gln Ser Xaa Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Ala Glu Phe Val 
    210                 215                 220                 


Thr Ala Glu Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  6
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Citrine 4-

<400>  6

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Glu Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asp 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Glu Tyr Gln Ser Ala Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Ala Glu Phe Val 
    210                 215                 220                 


Thr Ala Glu Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  7
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCitrine 4-

<400>  7

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Glu Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asp 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Glu Tyr Gln Ser Lys Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Ala Glu Phe Val 
    210                 215                 220                 


Thr Ala Glu Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  8
<211>  235
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCherry 5+

<400>  8

Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met 
1               5                   10                  15      


Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu 
            20                  25                  30          


Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Lys Gly Thr Gln Thr Ala 
        35                  40                  45              


Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile 
    50                  55                  60                  


Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro 
65                  70                  75                  80  


Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys 
                85                  90                  95      


Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr 
            100                 105                 110         


Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu 
        115                 120                 125             


Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr 
    130                 135                 140                 


Met Gly Trp Arg Lys Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala 
145                 150                 155                 160 


Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His 
                165                 170                 175     


Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln 
            180                 185                 190         


Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser His 
        195                 200                 205             


Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg 
    210                 215                 220                 


His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235 


<210>  9
<211>  235
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCherry 6+

<400>  9

Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met 
1               5                   10                  15      


Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu 
            20                  25                  30          


Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Lys Gly Thr Gln Thr Ala 
        35                  40                  45              


Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile 
    50                  55                  60                  


Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro 
65                  70                  75                  80  


Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys 
                85                  90                  95      


Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr 
            100                 105                 110         


Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu 
        115                 120                 125             


Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr 
    130                 135                 140                 


Met Gly Trp Arg Arg Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala 
145                 150                 155                 160 


Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His 
                165                 170                 175     


Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln 
            180                 185                 190         


Leu Pro Gly Ala Tyr Asn Val Arg Ile Lys Leu Asp Ile Thr Ser His 
        195                 200                 205             


Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg 
    210                 215                 220                 


His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235 


<210>  10
<211>  93
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FRB

<400>  10

Ile Leu Trp His Glu Met Trp His Glu Gly Leu Glu Glu Ala Ser Arg 
1               5                   10                  15      


Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe Glu Val Leu Glu 
            20                  25                  30          


Pro Leu His Ala Met Met Glu Arg Gly Pro Gln Thr Leu Lys Glu Thr 
        35                  40                  45              


Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala Gln Glu Trp 
    50                  55                  60                  


Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp Leu Leu Gln Ala 
65                  70                  75                  80  


Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Lys 
                85                  90              


<210>  11
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FKBP12

<400>  11

Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro 
1               5                   10                  15      


Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
            20                  25                  30          


Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe 
        35                  40                  45              


Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala 
    50                  55                  60                  


Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr 
65                  70                  75                  80  


Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr 
                85                  90                  95      


Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu 
            100                 105         


<210>  12
<211>  375
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FRB-20AA-mCherry 6+

<400>  12

Met Thr Gly Ile Leu Trp His Glu Met Trp His Glu Gly Leu Glu Glu 
1               5                   10                  15      


Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe Glu 
            20                  25                  30          


Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln Thr Leu 
        35                  40                  45              


Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala 
    50                  55                  60                  


Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp Leu 
65                  70                  75                  80  


Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Lys 
                85                  90                  95      


Thr Gly Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
            100                 105                 110         


Gly Ser Gly Ser Gly Ser Thr Gly Val Ser Lys Gly Glu Glu Asp Asn 
        115                 120                 125             


Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys Val His Met Glu Gly 
    130                 135                 140                 


Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu Gly Glu Gly Arg 
145                 150                 155                 160 


Pro Tyr Lys Gly Thr Gln Thr Ala Lys Leu Lys Val Thr Lys Gly Gly 
                165                 170                 175     


Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe Met Tyr Gly 
            180                 185                 190         


Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro Asp Tyr Leu Lys 
        195                 200                 205             


Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val Met Asn Phe Glu 
    210                 215                 220                 


Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser Leu Gln Asp Gly 
225                 230                 235                 240 


Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe Pro Ser Asp 
                245                 250                 255     


Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Arg Arg Ser Ser Glu 
            260                 265                 270         


Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys Gln Arg 
        275                 280                 285             


Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala Glu Val Lys Thr Thr 
    290                 295                 300                 


Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro Gly Ala Tyr Asn Val Arg 
305                 310                 315                 320 


Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr Thr Ile Val Glu 
                325                 330                 335     


Gln Tyr Glu Arg Ala Glu Gly Arg His Ser Thr Gly Gly Met Asp Glu 
            340                 345                 350         


Leu Tyr Lys Thr Gly Leu Glu Val Leu Phe Gln Gly Pro Thr Gly His 
        355                 360                 365             


His His His His His Thr Gly 
    370                 375 


<210>  13
<211>  392
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FKBP-20AA-citrine(206A) 4-

<400>  13

Met Thr Gly Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
1               5                   10                  15      


Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
            20                  25                  30          


Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro 
        35                  40                  45              


Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
    50                  55                  60                  


Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
65                  70                  75                  80  


Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
                85                  90                  95      


His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Thr Gly 
            100                 105                 110         


Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
        115                 120                 125             


Gly Ser Gly Ser Thr Gly Val Ser Lys Gly Glu Glu Leu Phe Thr Gly 
    130                 135                 140                 


Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys 
145                 150                 155                 160 


Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu 
                165                 170                 175     


Glu Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro 
            180                 185                 190         


Thr Leu Val Thr Thr Phe Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr 
        195                 200                 205             


Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu 
    210                 215                 220                 


Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr 
225                 230                 235                 240 


Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg 
                245                 250                 255     


Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly 
            260                 265                 270         


His Lys Leu Glu Tyr Asp Tyr Asn Ser His Asn Val Tyr Ile Met Ala 
        275                 280                 285             


Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn 
    290                 295                 300                 


Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr 
305                 310                 315                 320 


Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Glu 
                325                 330                 335     


Tyr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met 
            340                 345                 350         


Val Leu Ala Glu Phe Val Thr Ala Glu Gly Ile Thr Leu Gly Met Asp 
        355                 360                 365             


Glu Leu Tyr Lys Thr Gly Leu Glu Val Leu Phe Gln Gly Pro Thr Gly 
    370                 375                 380                 


His His His His His His Thr Gly 
385                 390         


<210>  14
<211>  365
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FRB-10AA-mCherry 6+

<400>  14

Met Thr Gly Ile Leu Trp His Glu Met Trp His Glu Gly Leu Glu Glu 
1               5                   10                  15      


Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe Glu 
            20                  25                  30          


Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln Thr Leu 
        35                  40                  45              


Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala 
    50                  55                  60                  


Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp Leu 
65                  70                  75                  80  


Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Lys 
                85                  90                  95      


Thr Gly Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Thr Gly Val Ser 
            100                 105                 110         


Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met Arg Phe 
        115                 120                 125             


Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu Ile Glu 
    130                 135                 140                 


Gly Glu Gly Glu Gly Arg Pro Tyr Lys Gly Thr Gln Thr Ala Lys Leu 
145                 150                 155                 160 


Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser 
                165                 170                 175     


Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp 
            180                 185                 190         


Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu 
        195                 200                 205             


Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp 
    210                 215                 220                 


Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly 
225                 230                 235                 240 


Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly 
                245                 250                 255     


Trp Arg Arg Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys 
            260                 265                 270         


Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His Tyr Asp 
        275                 280                 285             


Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro 
    290                 295                 300                 


Gly Ala Tyr Asn Val Arg Ile Lys Leu Asp Ile Thr Ser His Asn Glu 
305                 310                 315                 320 


Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg His Ser 
                325                 330                 335     


Thr Gly Gly Met Asp Glu Leu Tyr Lys Thr Gly Leu Glu Val Leu Phe 
            340                 345                 350         


Gln Gly Pro Thr Gly His His His His His His Thr Gly 
        355                 360                 365 


<210>  15
<211>  382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FKBP-10AA-mcitrine(206A) 4-

<400>  15

Met Thr Gly Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
1               5                   10                  15      


Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
            20                  25                  30          


Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro 
        35                  40                  45              


Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
    50                  55                  60                  


Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
65                  70                  75                  80  


Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
                85                  90                  95      


His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Thr Gly 
            100                 105                 110         


Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Thr Gly Val Ser Lys Gly 
        115                 120                 125             


Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly 
    130                 135                 140                 


Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp 
145                 150                 155                 160 


Ala Thr Tyr Gly Lys Leu Glu Leu Lys Phe Ile Cys Thr Thr Gly Lys 
                165                 170                 175     


Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe Gly Tyr Gly Leu 
            180                 185                 190         


Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe 
        195                 200                 205             


Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe 
    210                 215                 220                 


Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly 
225                 230                 235                 240 


Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu 
                245                 250                 255     


Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asp Tyr Asn Ser His 
            260                 265                 270         


Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn 
        275                 280                 285             


Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp 
    290                 295                 300                 


His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro 
305                 310                 315                 320 


Asp Asn His Tyr Leu Glu Tyr Gln Ser Ala Leu Ser Lys Asp Pro Asn 
                325                 330                 335     


Glu Lys Arg Asp His Met Val Leu Ala Glu Phe Val Thr Ala Glu Gly 
            340                 345                 350         


Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Thr Gly Leu Glu Val Leu 
        355                 360                 365             


Phe Gln Gly Pro Thr Gly His His His His His His Thr Gly 
    370                 375                 380         


<210>  16
<211>  375
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FR-20AA-mCherry

<400>  16

Met Thr Gly Ile Leu Trp His Glu Met Trp His Glu Gly Leu Glu Glu 
1               5                   10                  15      


Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe Glu 
            20                  25                  30          


Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln Thr Leu 
        35                  40                  45              


Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala 
    50                  55                  60                  


Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp Leu 
65                  70                  75                  80  


Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Lys 
                85                  90                  95      


Thr Gly Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
            100                 105                 110         


Gly Ser Gly Ser Gly Ser Thr Gly Val Ser Lys Gly Glu Glu Asp Asn 
        115                 120                 125             


Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys Val His Met Glu Gly 
    130                 135                 140                 


Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu Gly Glu Gly Arg 
145                 150                 155                 160 


Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys Val Thr Lys Gly Gly 
                165                 170                 175     


Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe Met Tyr Gly 
            180                 185                 190         


Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro Asp Tyr Leu Lys 
        195                 200                 205             


Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val Met Asn Phe Glu 
    210                 215                 220                 


Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser Leu Gln Asp Gly 
225                 230                 235                 240 


Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe Pro Ser Asp 
                245                 250                 255     


Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu Ala Ser Ser Glu 
            260                 265                 270         


Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys Gln Arg 
        275                 280                 285             


Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala Glu Val Lys Thr Thr 
    290                 295                 300                 


Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro Gly Ala Tyr Asn Val Asn 
305                 310                 315                 320 


Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr Thr Ile Val Glu 
                325                 330                 335     


Gln Tyr Glu Arg Ala Glu Gly Arg His Ser Thr Gly Gly Met Asp Glu 
            340                 345                 350         


Leu Tyr Lys Thr Gly Leu Glu Val Leu Phe Gln Gly Pro Thr Gly His 
        355                 360                 365             


His His His His His Thr Gly 
    370                 375 


<210>  17
<211>  392
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FKBP-20AA-Citrine

<400>  17

Met Thr Gly Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
1               5                   10                  15      


Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
            20                  25                  30          


Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro 
        35                  40                  45              


Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
    50                  55                  60                  


Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
65                  70                  75                  80  


Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
                85                  90                  95      


His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Thr Gly 
            100                 105                 110         


Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
        115                 120                 125             


Gly Ser Gly Ser Thr Gly Val Ser Lys Gly Glu Glu Leu Phe Thr Gly 
    130                 135                 140                 


Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys 
145                 150                 155                 160 


Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu 
                165                 170                 175     


Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro 
            180                 185                 190         


Thr Leu Val Thr Thr Phe Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr 
        195                 200                 205             


Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu 
    210                 215                 220                 


Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr 
225                 230                 235                 240 


Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg 
                245                 250                 255     


Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly 
            260                 265                 270         


His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala 
        275                 280                 285             


Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn 
    290                 295                 300                 


Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr 
305                 310                 315                 320 


Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser 
                325                 330                 335     


Tyr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met 
            340                 345                 350         


Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp 
        355                 360                 365             


Glu Leu Tyr Lys Thr Gly Leu Glu Val Leu Phe Gln Gly Pro Thr Gly 
    370                 375                 380                 


His His His His His His Thr Gly 
385                 390         


<210>  18
<211>  365
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FR-10AA-mCherry

<400>  18

Met Thr Gly Ile Leu Trp His Glu Met Trp His Glu Gly Leu Glu Glu 
1               5                   10                  15      


Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe Glu 
            20                  25                  30          


Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln Thr Leu 
        35                  40                  45              


Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala 
    50                  55                  60                  


Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp Leu 
65                  70                  75                  80  


Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Lys 
                85                  90                  95      


Thr Gly Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Thr Gly Val Ser 
            100                 105                 110         


Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe Met Arg Phe 
        115                 120                 125             


Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe Glu Ile Glu 
    130                 135                 140                 


Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu 
145                 150                 155                 160 


Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser 
                165                 170                 175     


Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp 
            180                 185                 190         


Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu 
        195                 200                 205             


Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp 
    210                 215                 220                 


Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly 
225                 230                 235                 240 


Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly 
                245                 250                 255     


Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys 
            260                 265                 270         


Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly His Tyr Asp 
        275                 280                 285             


Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro 
    290                 295                 300                 


Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser His Asn Glu 
305                 310                 315                 320 


Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg His Ser 
                325                 330                 335     


Thr Gly Gly Met Asp Glu Leu Tyr Lys Thr Gly Leu Glu Val Leu Phe 
            340                 345                 350         


Gln Gly Pro Thr Gly His His His His His His Thr Gly 
        355                 360                 365 


<210>  19
<211>  382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FKBP-10AA-Citrine

<400>  19

Met Thr Gly Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
1               5                   10                  15      


Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
            20                  25                  30          


Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro 
        35                  40                  45              


Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
    50                  55                  60                  


Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
65                  70                  75                  80  


Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
                85                  90                  95      


His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Thr Gly 
            100                 105                 110         


Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Thr Gly Val Ser Lys Gly 
        115                 120                 125             


Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly 
    130                 135                 140                 


Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp 
145                 150                 155                 160 


Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys 
                165                 170                 175     


Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe Gly Tyr Gly Leu 
            180                 185                 190         


Met Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe 
        195                 200                 205             


Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe 
    210                 215                 220                 


Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly 
225                 230                 235                 240 


Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu 
                245                 250                 255     


Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His 
            260                 265                 270         


Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn 
        275                 280                 285             


Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp 
    290                 295                 300                 


His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro 
305                 310                 315                 320 


Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala Leu Ser Lys Asp Pro Asn 
                325                 330                 335     


Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly 
            340                 345                 350         


Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Thr Gly Leu Glu Val Leu 
        355                 360                 365             


Phe Gln Gly Pro Thr Gly His His His His His His Thr Gly 
    370                 375                 380         


<210>  20
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (m)citrine


<220>
<221>  misc_feature
<222>  (616)..(618)
<223>  nnn can be gca or aaa

<400>  20
gtctccaaag gtgaagagct gttcacgggt gttgtgccga ttctggttga actggacggt       60

gacgtcaatg gccacaagtt tagcgtcagc ggtgagggcg agggtgatgc gacctacggt      120

aaactgacct tgaagtttat ctgcaccact ggcaaactgc cggtcccttg gccgaccctt      180

gttaccacgt tcggttacgg cctgatgtgc tttgcccgct atccagacca catgaaacaa      240

cacgactttt tcaaatctgc gatgccggaa ggctacgtac aagagcgtac cattttcttc      300

aaggacgatg gtaactacaa aacccgcgca gaggtcaaat tcgaaggtga taccttggtc      360

aaccgtattg agctgaaggg cattgacttc aaagaagatg gcaatatcct gggtcataag      420

ttggagtaca actataacag ccacaatgtt tacattatgg ccgacaaaca gaagaatggc      480

atcaaggtga atttcaaaat ccgtcataac atcgaggacg gttccgttca gctggcggat      540

cactatcagc agaatacccc gattggcgat ggcccggtgc tgctgccgga caaccattat      600

ctgagctatc agtccnnnct gagcaaagat ccgaatgaaa agcgcgacca catggtgctg      660

ttggaatttg ttaccgcagc gggcatcacg ctcggtatgg atgagctgta caaa            714


<210>  21
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Citrine

<400>  21
gtctccaaag gtgaagagct gttcacgggt gttgtgccga ttctggttga actggacggt       60

gacgtcaatg gccacaagtt tagcgtcagc ggtgagggcg agggtgatgc gacctacggt      120

aaactgacct tgaagtttat ctgcaccact ggcaaactgc cggtcccttg gccgaccctt      180

gttaccacgt tcggttacgg cctgatgtgc tttgcccgct atccagacca catgaaacaa      240

cacgactttt tcaaatctgc gatgccggaa ggctacgtac aagagcgtac cattttcttc      300

aaggacgatg gtaactacaa aacccgcgca gaggtcaaat tcgaaggtga taccttggtc      360

aaccgtattg agctgaaggg cattgacttc aaagaagatg gcaatatcct gggtcataag      420

ttggagtaca actataacag ccacaatgtt tacattatgg ccgacaaaca gaagaatggc      480

atcaaggtga atttcaaaat ccgtcataac atcgaggacg gttccgttca gctggcggat      540

cactatcagc agaatacccc gattggcgat ggcccggtgc tgctgccgga caaccattat      600

ctgagctatc agtccgcact gagcaaagat ccgaatgaaa agcgcgacca catggtgctg      660

ttggaatttg ttaccgcagc gggcatcacg ctcggtatgg atgagctgta caaa            714


<210>  22
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCitrine

<400>  22
gtctccaaag gtgaagagct gttcacgggt gttgtgccga ttctggttga actggacggt       60

gacgtcaatg gccacaagtt tagcgtcagc ggtgagggcg agggtgatgc gacctacggt      120

aaactgacct tgaagtttat ctgcaccact ggcaaactgc cggtcccttg gccgaccctt      180

gttaccacgt tcggttacgg cctgatgtgc tttgcccgct atccagacca catgaaacaa      240

cacgactttt tcaaatctgc gatgccggaa ggctacgtac aagagcgtac cattttcttc      300

aaggacgatg gtaactacaa aacccgcgca gaggtcaaat tcgaaggtga taccttggtc      360

aaccgtattg agctgaaggg cattgacttc aaagaagatg gcaatatcct gggtcataag      420

ttggagtaca actataacag ccacaatgtt tacattatgg ccgacaaaca gaagaatggc      480

atcaaggtga atttcaaaat ccgtcataac atcgaggacg gttccgttca gctggcggat      540

cactatcagc agaatacccc gattggcgat ggcccggtgc tgctgccgga caaccattat      600

ctgagctatc agtccaaact gagcaaagat ccgaatgaaa agcgcgacca catggtgctg      660

ttggaatttg ttaccgcagc gggcatcacg ctcggtatgg atgagctgta caaa            714


<210>  23
<211>  705
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCherry

<400>  23
gttagcaagg gtgaagaaga taacatggca atcatcaaag agtttatgcg tttcaaggtg       60

cacatggagg gtagcgttaa cggtcacgag ttcgaaattg aaggtgaggg tgagggccgt      120

ccgtacgaag gtacccaaac cgccaaactg aaggttacga agggtggccc gttgccgttt      180

gcgtgggata ttctgagccc gcagttcatg tatggtagca aggcgtatgt aaagcaccca      240

gcggacatcc cggactatct gaaactgtcg tttccggaag gtttcaaatg ggagcgcgtc      300

atgaatttcg aggatggcgg cgttgtcacc gtgacccagg atagctccct gcaagatggc      360

gagttcattt acaaggtcaa actgcgcggc accaatttcc cgtccgacgg tccggttatg      420

caaaagaaaa ccatgggctg ggaagctagc agcgaaagaa tgtatccgga agatggtgca      480

ctgaagggtg agatcaaaca acgtctgaag ctgaaggacg gcggtcatta cgacgccgag      540

gtgaaaacca cctataaggc gaaaaagccg gttcagctgc cgggtgcgta taacgtcaat      600

atcaaactgg atatcacgag ccacaatgag gactacacga ttgtggagca atacgaacgc      660

gcggaaggtc gtcattctac gggtggcatg gacgaactgt acaag                      705


<210>  24
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (m)citrine 4-


<220>
<221>  misc_feature
<222>  (616)..(618)
<223>  nnn can be gca or aaa

<400>  24
gtctccaaag gtgaagagct gttcaccggc gttgtgccga ttctggttga actggacggt       60

gacgtcaatg gccacaagtt tagcgtcagc ggtgagggcg agggtgatgc gacctacggt      120

aaactggagt tgaagtttat ctgcaccact ggcaaactgc cggtcccttg gccgaccctt      180

gttaccacgt tcggttacgg cctgatgtgc tttgcccgct atccagacca catgaaacaa      240

cacgactttt tcaaatctgc gatgccggaa ggctacgtac aagagcgtac cattttcttc      300

aaggacgatg gtaactacaa aacccgcgca gaggtcaaat tcgaaggtga taccttggtc      360

aaccgtattg agctgaaggg cattgacttc aaagaagatg gcaatatcct gggtcataag      420

ttggagtacg actataacag ccacaatgtt tacattatgg ccgacaaaca gaagaatggc      480

atcaaggtga atttcaaaat ccgtcataac atcgaggacg gttccgttca gctggcggat      540

cactatcagc agaatacccc gattggcgat ggcccggtgc tgctgccgga caaccattat      600

ctggagtatc agtccnnnct gagcaaagat ccgaatgaaa agcgcgacca catggtgctg      660

gcggagttcg ttaccgcaga gggcatcacg ctcggtatgg atgagctgta caaa            714


<210>  25
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Citrine (206A) 4-

<400>  25
gtctccaaag gtgaagagct gttcaccggc gttgtgccga ttctggttga actggacggt       60

gacgtcaatg gccacaagtt tagcgtcagc ggtgagggcg agggtgatgc gacctacggt      120

aaactggagt tgaagtttat ctgcaccact ggcaaactgc cggtcccttg gccgaccctt      180

gttaccacgt tcggttacgg cctgatgtgc tttgcccgct atccagacca catgaaacaa      240

cacgactttt tcaaatctgc gatgccggaa ggctacgtac aagagcgtac cattttcttc      300

aaggacgatg gtaactacaa aacccgcgca gaggtcaaat tcgaaggtga taccttggtc      360

aaccgtattg agctgaaggg cattgacttc aaagaagatg gcaatatcct gggtcataag      420

ttggagtacg actataacag ccacaatgtt tacattatgg ccgacaaaca gaagaatggc      480

atcaaggtga atttcaaaat ccgtcataac atcgaggacg gttccgttca gctggcggat      540

cactatcagc agaatacccc gattggcgat ggcccggtgc tgctgccgga caaccattat      600

ctggagtatc agtccgcact gagcaaagat ccgaatgaaa agcgcgacca catggtgctg      660

gcggagttcg ttaccgcaga gggcatcacg ctcggtatgg atgagctgta caaa            714


<210>  26
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCitrine (206K) 4-

<400>  26
gtctccaaag gtgaagagct gttcaccggc gttgtgccga ttctggttga actggacggt       60

gacgtcaatg gccacaagtt tagcgtcagc ggtgagggcg agggtgatgc gacctacggt      120

aaactggagt tgaagtttat ctgcaccact ggcaaactgc cggtcccttg gccgaccctt      180

gttaccacgt tcggttacgg cctgatgtgc tttgcccgct atccagacca catgaaacaa      240

cacgactttt tcaaatctgc gatgccggaa ggctacgtac aagagcgtac cattttcttc      300

aaggacgatg gtaactacaa aacccgcgca gaggtcaaat tcgaaggtga taccttggtc      360

aaccgtattg agctgaaggg cattgacttc aaagaagatg gcaatatcct gggtcataag      420

ttggagtacg actataacag ccacaatgtt tacattatgg ccgacaaaca gaagaatggc      480

atcaaggtga atttcaaaat ccgtcataac atcgaggacg gttccgttca gctggcggat      540

cactatcagc agaatacccc gattggcgat ggcccggtgc tgctgccgga caaccattat      600

ctggagtatc agtccaaact gagcaaagat ccgaatgaaa agcgcgacca catggtgctg      660

gcggagttcg ttaccgcaga gggcatcacg ctcggtatgg atgagctgta caaa            714


<210>  27
<211>  705
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCherry 5+

<400>  27
gttagcaagg gtgaagaaga taacatggca atcatcaaag agtttatgcg tttcaaggtg       60

cacatggagg gtagcgttaa cggtcacgag ttcgaaattg aaggtgaggg tgagggccgt      120

ccgtacaaag gtacccaaac cgccaaactg aaggttacga agggtggccc gttgccgttt      180

gcgtgggata ttctgagccc gcagttcatg tatggtagca aggcgtatgt aaagcaccca      240

gcggacatcc cggactatct gaaactgtcg tttccggaag gtttcaaatg ggagcgcgtc      300

atgaatttcg aggatggcgg cgttgtcacc gtgacccagg atagctccct gcaagatggc      360

gagttcattt acaaggtcaa actgcgcggc accaatttcc cgtccgacgg tccggttatg      420

caaaagaaaa ccatgggctg gcgtaaaagc agcgaaagaa tgtatccgga agatggtgca      480

ctgaagggtg agatcaaaca acgtctgaag ctgaaggacg gcggtcatta cgacgccgag      540

gtgaaaacca cctataaggc gaaaaagccg gttcagctgc cgggtgcgta taacgtcaat      600

atcaaactgg atatcacgag ccacaatgag gactacacga ttgtggagca atacgaacgc      660

gcggaaggtc gtcattctac gggtggcatg gacgaactgt acaag                      705


<210>  28
<211>  705
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCherry 6+

<400>  28
gttagcaagg gtgaagaaga taacatggca atcatcaaag agtttatgcg tttcaaggtg       60

cacatggagg gtagcgttaa cggtcacgag ttcgaaattg aaggtgaggg tgagggccgt      120

ccgtacaaag gtacccaaac cgccaaactg aaggttacga agggtggccc gttgccgttt      180

gcgtgggata ttctgagccc gcagttcatg tatggtagca aggcgtatgt aaagcaccca      240

gcggacatcc cggactatct gaaactgtcg tttccggaag gtttcaaatg ggagcgcgtc      300

atgaatttcg aggatggcgg cgttgtcacc gtgacccagg atagctccct gcaagatggc      360

gagttcattt acaaggtcaa actgcgcggc accaatttcc cgtccgacgg tccggttatg      420

caaaagaaaa ccatgggctg gcgtcgtagc agcgaaagaa tgtatccgga agatggtgca      480

ctgaagggtg agatcaaaca acgtctgaag ctgaaggacg gcggtcatta cgacgccgag      540

gtgaaaacca cctataaggc gaaaaagccg gttcagctgc cgggtgcgta taacgtccgt      600

atcaaactgg atatcacgag ccacaatgag gactacacga ttgtggagca atacgaacgc      660

gcggaaggtc gtcattctac gggtggcatg gacgaactgt acaag                      705


<210>  29
<211>  279
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FRB

<400>  29
atcctctggc atgaaatgtg gcacgagggt ttagaagagg ccagccgtct gtatttcggc       60

gagcgcaacg tgaaaggcat gtttgaggtg ctggaacctc tgcacgctat gatggagcgt      120

ggcccgcaga ctttgaaaga aaccagcttt aaccaggcgt acggccgtga tctgatggag      180

gcacaggagt ggtgccgtaa gtacatgaaa agcggtaatg tcaaagacct gttgcaagca      240

tgggacctgt actaccacgt gtttcgtcgc attagcaaa                             279


<210>  30
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FKBP12

<400>  30
ggtgtgcaag ttgaaacgat tagcccaggt gatggtcgta ccttcccgaa acgtggtcag       60

acgtgtgtgg tgcattacac gggtatgctg gaggacggta agaagttcga cagcagccgt      120

gatcgtaaca agccgtttaa gtttatgctg ggtaagcaag aagtgattcg tggttgggaa      180

gaaggtgtgg ctcaaatgag cgtcggccag cgcgcgaagc tgaccatcag cccggattac      240

gcgtatggtg caaccggcca cccgggcatt atcccgccac atgctacgct ggttttcgac      300

gttgagctgc tgaagttaga g                                                321


<210>  31
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  [GS]5 10aa linker

<400>  31

Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
1               5                   10  


<210>  32
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  [GS]10 20aa linker

<400>  32

Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
1               5                   10                  15      


Gly Ser Gly Ser 
            20  


<210>  33
<211>  50
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  50aa randomized synthetic linker

<400>  33

Thr Gly Ser Ser Gly Ser Ser Gly Ser Ser Thr Ser Glu Gly Gln Glu 
1               5                   10                  15      


Gly Thr Gln Thr Gly Ser Thr Ser Thr Ser Gly Thr Gly Thr Gln Ser 
            20                  25                  30          


Gly Thr Gly Gly Thr Gln Ser Gly Thr Glu Thr Ser Thr Gly Ser Gly 
        35                  40                  45              


Glu Thr 
    50  


<210>  34
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Factor Xa cleavage site

<400>  34

Ile Glu Gly Arg 
1               


<210>  35
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  the thrombin cleavage site

<400>  35

Leu Val Pro Arg 
1               


<210>  36
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  the enterokinase cleaving site

<400>  36

Asp Asp Asp Asp Lys 
1               5   


<210>  37
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PreScission cleavage site

<400>  37

Leu Glu Val Leu Phe Gln Gly Pro 
1               5               


