                         SEQUENCE LISTING

<110>  Duke University
 
<120>  EXPRESSION OF G-PROTEIN COUPLED RECEPTORS (GPCRs)

<130>  028193-9125-WO00

<150>  US 61/564,964
<151>  2011-11-30

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  361
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ala Met Asn Tyr Asn Ala Lys Asp Glu Val Asp Gly Gly Pro Pro 
1               5                   10                  15      


Cys Ala Pro Gly Gly Thr Ala Lys Thr Arg Arg Pro Asp Asn Thr Ala 
            20                  25                  30          


Phe Lys Gln Gln Arg Leu Pro Ala Trp Gln Pro Ile Leu Thr Ala Gly 
        35                  40                  45              


Thr Val Leu Pro Ile Phe Phe Ile Ile Gly Leu Ile Phe Ile Pro Ile 
    50                  55                  60                  


Gly Ile Gly Ile Phe Val Thr Ser Asn Asn Ile Arg Glu Ile Glu Ile 
65                  70                  75                  80  


Asp Tyr Thr Gly Thr Glu Pro Ser Ser Pro Cys Asn Lys Cys Leu Ser 
                85                  90                  95      


Pro Asp Val Thr Pro Cys Phe Cys Thr Ile Asn Phe Thr Leu Glu Lys 
            100                 105                 110         


Ser Phe Glu Gly Asn Val Phe Met Tyr Tyr Gly Leu Ser Asn Phe Tyr 
        115                 120                 125             


Gln Asn His Arg Arg Tyr Val Lys Ser Arg Asp Asp Ser Gln Leu Asn 
    130                 135                 140                 


Gly Asp Ser Ser Ala Leu Leu Asn Pro Ser Lys Glu Cys Glu Pro Tyr 
145                 150                 155                 160 


Arg Arg Asn Glu Asp Lys Pro Ile Ala Pro Cys Gly Ala Ile Ala Asn 
                165                 170                 175     


Ser Met Phe Asn Asp Thr Leu Glu Leu Phe Leu Ile Gly Asn Asp Ser 
            180                 185                 190         


Tyr Pro Ile Pro Ile Ala Leu Lys Lys Lys Gly Ile Ala Trp Trp Thr 
        195                 200                 205             


Asp Lys Asn Val Lys Phe Arg Asn Pro Pro Gly Gly Asp Asn Leu Glu 
    210                 215                 220                 


Glu Arg Phe Lys Gly Thr Thr Lys Pro Val Asn Trp Leu Lys Pro Val 
225                 230                 235                 240 


Tyr Met Leu Asp Ser Asp Pro Asp Asn Asn Gly Phe Ile Asn Glu Asp 
                245                 250                 255     


Phe Ile Val Trp Met Arg Thr Ala Ala Leu Pro Thr Phe Arg Lys Leu 
            260                 265                 270         


Tyr Arg Leu Ile Glu Arg Lys Ser Asp Leu His Pro Thr Leu Pro Ala 
        275                 280                 285             


Gly Arg Tyr Ser Leu Asn Val Thr Tyr Asn Tyr Pro Val His Tyr Phe 
    290                 295                 300                 


Asp Gly Arg Lys Arg Met Ile Leu Ser Thr Ile Ser Trp Met Gly Gly 
305                 310                 315                 320 


Lys Asn Pro Phe Leu Gly Ile Ala Tyr Ile Ala Val Gly Ser Ile Ser 
                325                 330                 335     


Phe Leu Leu Gly Val Val Leu Leu Val Ile Asn His Lys Tyr Arg Asn 
            340                 345                 350         


Ser Ser Asn Thr Ala Asp Ile Thr Ile 
        355                 360     


<210>  2
<211>  1086
<212>  DNA
<213>  Homo sapiens

<400>  2
atggcgatga actataacgc gaaggatgaa gtggacggtg ggcccccgtg tgctccgggg       60

ggcaccgcga agactcggag accggataac acggccttca aacagcaacg gctgccagct      120

tggcagccca tccttacggc tggcacggtg ctacctattt tcttcatcat cggtctcatc      180

ttcattccca tcggcattgg catttttgtc acctccaaca acatccgcga gatcgagatt      240

gattataccg gaacagagcc ttccagtccc tgtaataaat gtttatctcc ggatgtgaca      300

ccttgctttt gtaccattaa cttcacactg gaaaagtcat ttgagggcaa cgtgtttatg      360

tattatggac tgtctaattt ctatcaaaac catcgtcgtt acgtgaaatc tcgagatgat      420

agtcaactaa atggagattc tagtgctttg cttaatccca gtaaggaatg tgaaccttat      480

cgaagaaatg aagacaaacc aattgctcct tgtggagcta ttgccaacag catgtttaat      540

gatacattag aattgtttct cattggcaat gattcttatc ctatacctat cgctttgaaa      600

aagaaaggta ttgcttggtg gacagataaa aatgtgaaat tcagaaatcc ccctggagga      660

gacaacctgg aagaacgatt taaaggtaca acaaagcctg tgaactggct taaaccagtt      720

tacatgctgg attctgaccc agataataat ggattcataa atgaggattt tattgtttgg      780

atgcgtactg cagcattacc tacttttcgc aagttgtatc gtcttataga aaggaaaagt      840

gatttacatc caacattacc agctggccga tactctttga atgtcacata caattaccct      900

gtacattatt ttgatggacg aaaacggatg atcttgagca ctatttcatg gatgggagga      960

aaaaatccat ttttggggat tgcttacatc gctgttggat ccatctcctt ccttctggga     1020

gttgtactgc tagtaattaa tcataaatat agaaacagta gtaatacagc tgacattacc     1080

atttaa                                                                1086


<210>  3
<211>  364
<212>  PRT
<213>  Mus musculus

<400>  3

Met Ala Met Asn Tyr Ser Ala Lys Asp Glu Val Asp Gly Gly Pro Ala 
1               5                   10                  15      


Gly Pro Pro Gly Gly Ala Ala Lys Thr Arg Arg Pro Asp Asn Thr Ala 
            20                  25                  30          


Phe Lys Gln Gln Arg Leu Pro Ala Trp Gln Pro Ile Leu Thr Ala Gly 
        35                  40                  45              


Thr Val Leu Pro Thr Phe Phe Ile Ile Gly Leu Ile Phe Ile Pro Ile 
    50                  55                  60                  


Gly Ile Gly Ile Phe Val Thr Ser Asn Asn Ile Arg Glu Ile Glu Ile 
65                  70                  75                  80  


Asp Tyr Thr Gly Thr Glu Pro Ser Ser Pro Cys Asn Lys Cys Leu Ser 
                85                  90                  95      


Pro Asn Val Thr Ser Cys Ala Cys Thr Ile Asn Phe Thr Leu Lys Gln 
            100                 105                 110         


Ser Phe Glu Gly Asn Val Phe Met Tyr Tyr Gly Leu Ser Asn Phe Tyr 
        115                 120                 125             


Gln Asn His Arg Arg Tyr Val Lys Ser Arg Asp Asp Ser Gln Leu Asn 
    130                 135                 140                 


Gly Asp Pro Ser Ala Leu Leu Asn Pro Ser Lys Glu Cys Glu Pro Tyr 
145                 150                 155                 160 


Arg Arg Asn Glu Asp Arg Pro Ile Ala Pro Cys Gly Ala Ile Ala Asn 
                165                 170                 175     


Ser Met Phe Asn Asp Thr Leu Glu Leu Tyr Leu Val Ala Asn Glu Ser 
            180                 185                 190         


Asp Pro Lys Pro Ile Pro Ile Pro Leu Lys Lys Lys Gly Ile Ala Trp 
        195                 200                 205             


Trp Thr Asp Lys Asn Val Lys Phe Arg Asn Pro Pro Gly Lys Glu Ser 
    210                 215                 220                 


Leu Glu Glu Lys Phe Lys Asp Thr Ile Lys Pro Val Asn Trp His Lys 
225                 230                 235                 240 


Ala Val Tyr Glu Leu Asp Pro Glu Asp Glu Ser Asn Asn Gly Phe Ile 
                245                 250                 255     


Asn Glu Asp Phe Ile Val Trp Met Arg Thr Ala Ala Leu Pro Thr Phe 
            260                 265                 270         


Arg Lys Leu Tyr Arg Leu Ile Glu Arg Arg Asp Asp Leu His Pro Thr 
        275                 280                 285             


Leu Pro Ala Gly Gln Tyr Phe Leu Asn Ile Thr Tyr Asn Tyr Pro Val 
    290                 295                 300                 


His Ser Phe Asp Gly Arg Lys Arg Met Ile Leu Ser Thr Ile Ser Trp 
305                 310                 315                 320 


Met Gly Gly Lys Asn Pro Phe Leu Gly Ile Ala Tyr Ile Thr Ile Gly 
                325                 330                 335     


Ser Ile Ser Phe Leu Leu Gly Val Val Leu Leu Val Ile Asn His Lys 
            340                 345                 350         


Tyr Arg Asn Ser Ser Asn Thr Ala Asp Ile Thr Ile 
        355                 360                 


<210>  4
<211>  995
<212>  DNA
<213>  Mus musculus

<400>  4
atggcgatga actatagcgc gaaggatgag gtggacggcg ggcccgcggg tcctcccggg       60

ggcgccgcca agacccggag gccggataac acggccttca aacagcaacg gctacccgcc      120

tggcagccca tcctcacggc cggcacggtg ctgcccacct tcttcatcat cggcctcatc      180

ttcatcccca tcggcatcgg catcttcgtc acctccaaca acatccgtga gatcgagatt      240

gattacactg gaacagaacc ttccagcccc tgcaataaat gtttatctcc gaatgtgaca      300

tcttgtgctt gtaccattaa cttcacactg aaacagtcat ttgagggcaa tgtgtttatg      360

tattatggac tgtctaattt ctatcaaaat catcgtcgct acgtgaaatc tcgagatgat      420

agccagttaa atggagaccc tagtgctttg cttaatccaa gtaaggaatg tgaaccttat      480

agaagaaatg aagacagacc aattgcgcca tgtggcgcta ttgccaacag catgtttaat      540

gatacgttag aattgtatct ggttgccaat gaatctgatc ccaagcctat tccaattcct      600

ttgaagaaaa aaggtattgc ttggtggaca gataaaaatg tgaaattcag aaatccacct      660

ggaaaagaga gcctcgaaga aaagtttaaa gatacaataa agccagtaaa ctggcataag      720

gcagtatatg agctagaccc tgaagatgaa agtaataatg gattcataaa tgaagacttt      780

atagtttgga tgcgtactgc agcattacct acttttcgta agttgtatcg tctcatagag      840

cggagagatg atttacaccc aacattacca gctggacagt actttttgaa catcacatac      900

aattaccctg tgcattcctt tgatggacgg aaacggatga tcttgagcac tatttcatgg      960

atgggaggaa agaatccatt tttgggaatt gctta                                 995


<210>  5
<211>  348
<212>  PRT
<213>  Caenorhabditis elegans

<400>  5

Met Pro Pro Arg Asp Ala Val Pro Thr Ser Thr Gln Val Ser Gly Ile 
1               5                   10                  15      


Gly Ala Asp Gly Val Gln Thr Glu Lys Val Leu Lys Asn Arg Pro Lys 
            20                  25                  30          


Ala Ser Ala Leu Arg Gln Gln Lys Leu Pro Ala Trp Gln Pro Ile Leu 
        35                  40                  45              


Thr Ala Thr Thr Val Ile Pro Thr Val Phe Val Ile Gly Ala Ile Phe 
    50                  55                  60                  


Leu Pro Ile Gly Val Phe Leu Phe Ile Ala Ser Asp Ala Val Ser Glu 
65                  70                  75                  80  


Phe Thr Val Glu Tyr Thr Asn Cys Leu Ser Pro Cys Gln Leu Gln Ile 
                85                  90                  95      


Asn Leu Pro Asn Ala Phe Asp Gly Asp Val Phe Leu Tyr Tyr Asn Leu 
            100                 105                 110         


Glu Asn Tyr Tyr Gln Asn His Arg Arg Tyr Val Lys Ser Arg Asn Asp 
        115                 120                 125             


Gln Gln Tyr Leu Gly Asp Leu Thr Asn Val Lys Asp Cys Ala Pro Phe 
    130                 135                 140                 


Asp Ile Asp Pro Ala Thr Lys Lys Pro Ile Ala Pro Cys Gly Ala Ile 
145                 150                 155                 160 


Ala Asn Ser Ile Phe Asn Asp Thr Phe Thr Leu Ala His Arg Ala Asp 
                165                 170                 175     


Thr Gly Ile Val Thr Met Val Pro Val Thr Thr Gln Gly Val Ile Trp 
            180                 185                 190         


Asn Val Asp Lys Asp Arg Lys Phe Lys Asn Pro Pro Leu Asn Asp Gly 
        195                 200                 205             


Asn Leu Cys Asp Ala Phe Asn Asn Thr Thr Lys Pro Pro Asn Trp Ser 
    210                 215                 220                 


Lys Asn Pro Cys Glu Val Gly Gly Phe Glu Asn Val Asp Phe Ile Val 
225                 230                 235                 240 


Trp Met Arg Thr Ala Ala Leu Pro Tyr Phe Lys Lys Leu Trp Arg Ile 
                245                 250                 255     


Val Asp Arg Thr Thr Asn Pro Leu Phe Ser Asn Gly Leu Pro Gln Gly 
            260                 265                 270         


Thr Tyr Ile Leu Thr Val Glu Asn Asn Tyr Pro Val Gln Ser Phe Gly 
        275                 280                 285             


Gly Lys Lys Glu Phe Val Ile Ser Thr Thr Ser Trp Ala Gly Gly Lys 
    290                 295                 300                 


Asn Ser Phe Leu Gly Ile Ala Tyr Leu Val Val Gly Ser Leu Ala Ile 
305                 310                 315                 320 


Val Leu Gly Val Val Phe Ile Val Ile His Met Lys Phe Gly His Ser 
                325                 330                 335     


Met Asn Glu Leu Ser Asn Val Ser Glu Ile His His 
            340                 345             


<210>  6
<211>  1047
<212>  DNA
<213>  Caenorhabditis elegans

<400>  6
atgccgccga gagacgccgt tccgacgtcg acgcaagtga gtggtattgg tgctgacggg       60

gttcaaacgg aaaaagtgct gaaaaatcgg ccgaaggcaa gcgcccttcg acagcaaaaa      120

ctccccgcat ggcaaccgat tctcacagca accacagtaa ttccaacggt tttcgtcatc      180

ggagccatct ttctacccat cggcgtcttc ctattcatcg cttccgacgc ggtttccgag      240

ttcaccgtcg aatacaccaa ttgcttatca ccgtgccaat tgcaaattaa tttaccaaat      300

gcgttcgacg gagacgtctt cttgtactat aatctcgaga attactatca aaatcatcgg      360

cgatacgtga aaagccggaa tgatcagcaa tatcttggag atttgacgaa cgtgaaggac      420

tgtgctccat tcgacattga ccctgcgaca aagaagccaa ttgctccatg cggtgcgatt      480

gccaattcga tattcaacga cactttcacg ctggctcatc gtgccgacac aggcatcgtt      540

accatggtac ccgtgacgac tcagggtgtc atctggaatg tggacaagga tcggaaattc      600

aagaatcccc cgttgaatga tggcaacctc tgtgacgcat tcaacaatac cacgaaacca      660

ccgaattgga gcaaaaatcc gtgtgaagtt ggcggttttg agaacgtgga tttcattgtt      720

tggatgcgaa cggcagctct tccatatttc aaaaagctgt ggagaatagt cgaccggacc      780

acgaatccat tgttttcaaa tggactcccc cagggaactt atattttgac agtcgagaat      840

aactacccag ttcaatcatt cggcggaaag aaagagtttg tgatatcgac aacaagttgg      900

gctggaggca agaactcatt cctcggaata gcctatctcg tcgtcggatc cctggccatt      960

gtgctcggcg tagtcttcat cgtgattcac atgaagtttg gacattcgat gaacgagttg     1020

agcaacgtct cggaaattca tcactga                                         1047


<210>  7
<211>  391
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  7

Met Val Ser Leu Phe Lys Arg Gly Lys Ala Pro Pro Leu Thr Lys Glu 
1               5                   10                  15      


Gly Pro Thr Ser Lys Lys Pro Pro Asn Thr Ala Phe Arg Gln Gln Arg 
            20                  25                  30          


Leu Lys Ala Trp Gln Pro Ile Leu Ser Pro Gln Ser Val Leu Pro Leu 
        35                  40                  45              


Leu Ile Phe Val Ala Cys Ile Phe Thr Pro Ile Gly Ile Gly Leu Ile 
    50                  55                  60                  


Val Ser Ala Thr Lys Val Gln Asp Leu Thr Ile Asp Tyr Ser His Cys 
65                  70                  75                  80  


Asp Thr Lys Ala Ser Thr Thr Ala Phe Glu Asp Ile Pro Lys Lys Tyr 
                85                  90                  95      


Ile Lys Tyr His Phe Lys Ser Lys Val Glu Asn Lys Pro Gln Trp Arg 
            100                 105                 110         


Leu Thr Glu Asn Glu Asn Gly Glu Gln Ser Cys Glu Leu Gln Phe Glu 
        115                 120                 125             


Ile Pro Asn Asp Ile Lys Lys Ser Ile Phe Ile Tyr Tyr Lys Ile Thr 
    130                 135                 140                 


Asn Phe Tyr Gln Asn His Arg Arg Tyr Val Gln Ser Phe Asp Thr Lys 
145                 150                 155                 160 


Gln Ile Leu Gly Glu Pro Ile Lys Lys Asp Asp Leu Asp Thr Ser Cys 
                165                 170                 175     


Ser Pro Ile Arg Ser Arg Glu Asp Lys Ile Ile Tyr Pro Cys Gly Leu 
            180                 185                 190         


Ile Ala Asn Ser Met Phe Asn Asp Thr Phe Ser Gln Val Leu Ser Gly 
        195                 200                 205             


Ile Asp Asp Thr Glu Asp Tyr Asn Leu Thr Asn Lys His Ile Ser Trp 
    210                 215                 220                 


Ser Ile Asp Arg His Arg Phe Lys Thr Thr Lys Tyr Asn Ala Ser Asp 
225                 230                 235                 240 


Ile Val Pro Pro Pro Asn Trp Met Lys Lys Tyr Pro Asp Gly Tyr Thr 
                245                 250                 255     


Asp Glu Asn Leu Pro Asp Ile His Thr Trp Glu Glu Phe Gln Val Trp 
            260                 265                 270         


Met Arg Thr Ala Ala Phe Pro Lys Phe Tyr Lys Leu Thr Leu Lys Asn 
        275                 280                 285             


Glu Ser Ala Ser Leu Pro Lys Gly Lys Tyr Gln Met Asn Ile Glu Leu 
    290                 295                 300                 


Asn Tyr Pro Ile Ser Leu Phe Gly Gly Thr Lys Ser Phe Val Leu Thr 
305                 310                 315                 320 


Thr Asn Gly Ala Ile Gly Gly Arg Asn Met Ser Leu Gly Val Leu Tyr 
                325                 330                 335     


Leu Ile Val Ala Gly Leu Cys Ala Leu Phe Gly Ile Ile Phe Leu Val 
            340                 345                 350         


Lys Leu Ile Phe Gln Pro Arg Ala Met Gly Asp His Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Asp Glu Glu Asn Glu Asp Tyr Glu Asp Val His Ala Glu Asn 
    370                 375                 380                 


Thr Thr Leu Arg Glu Ile Leu 
385                 390     


<210>  8
<211>  1176
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  8
atggtttcat tgttcaaaag aggtaaggct ccaccgctca cgaaagaagg ccccacttct       60

aaaaagcctc ctaacacagc gtttagacaa caaaggctta aggcatggca accaatactg      120

tctcctcaaa gtgtgcttcc gttgttaata ttcgttgcat gtatatttac tcctattggt      180

attggactca ttgtaagcgc tactaaggta caagatctaa caattgatta tagtcattgt      240

gatacaaaag catctacaac tgcttttgaa gatataccaa agaagtacat taaatatcac      300

tttaaaagta aagttgaaaa taaaccacaa tggaggctaa ccgaaaatga aaatggcgaa      360

caatcatgcg aactgcagtt cgaaatccca aacgatatca agaaatccat ttttatatat      420

tataaaataa ccaattttta tcaaaatcat cgcagatatg tccaatcgtt tgacacaaag      480

caaatattag gggagcctat caaaaaagat gatctggata caagctgtag tccaataaga      540

agtagggaag acaaaataat atatccctgt gggttgatcg ctaattccat gtttaatgat      600

acattttctc aggtgttgag tggtatagat gacacagaag actataattt aactaacaag      660

catatatcat ggagtattga tcgtcacaga tttaaaacca ccaagtataa tgctagcgat      720

attgttccac cgccaaactg gatgaagaag tatcccgatg ggtatacaga tgaaaatctt      780

cctgatatcc atacttggga agagttccag gtatggatga ggactgcagc ctttcccaag      840

ttttacaagt tgacgttgaa aaatgaatct gcttctttac cgaagggtaa atatcaaatg      900

aacattgagt tgaattatcc gatttcactc tttggtggca caaaatcatt tgtactgact      960

acaaatggag ctattggtgg tagaaatatg tcactaggcg tactgtacct catcgttgca     1020

gggctttgcg ccttatttgg catcattttt ttggttaaat taatcttcca accaagagcg     1080

atgggtgatc acacttattt gaattttgat gatgaagaaa acgaggatta tgaggatgta     1140

cacgcagaga atacaacatt gagggaaatt ttatag                               1176


