                         SEQUENCE LISTING

<110>  Novozymes A/S
       Deinhammer, Randall
       Craig, Joyce
       Matsui, Tomoko
       Takagi, Shinobu
       Clark, Suzanne
       Matthews, John
       Hjulmand, Anne  Glud
       Soong, Chee-Leong
 
<120>  Processes for Producing Fermentation Products

<130>  12075-WO-PCT

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  515
<212>  PRT
<213>  Bacillus stearothermophilus


<220>
<221>  mat_peptide
<222>  (1)..(515)

<400>  1

Ala Ala Pro Phe Asn Gly Thr Met Met Gln Tyr Phe Glu Trp Tyr Leu 
1               5                   10                  15      


Pro Asp Asp Gly Thr Leu Trp Thr Lys Val Ala Asn Glu Ala Asn Asn 
            20                  25                  30          


Leu Ser Ser Leu Gly Ile Thr Ala Leu Trp Leu Pro Pro Ala Tyr Lys 
        35                  40                  45              


Gly Thr Ser Arg Ser Asp Val Gly Tyr Gly Val Tyr Asp Leu Tyr Asp 
    50                  55                  60                  


Leu Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly Thr 
65                  70                  75                  80  


Lys Ala Gln Tyr Leu Gln Ala Ile Gln Ala Ala His Ala Ala Gly Met 
                85                  90                  95      


Gln Val Tyr Ala Asp Val Val Phe Asp His Lys Gly Gly Ala Asp Gly 
            100                 105                 110         


Thr Glu Trp Val Asp Ala Val Glu Val Asn Pro Ser Asp Arg Asn Gln 
        115                 120                 125             


Glu Ile Ser Gly Thr Tyr Gln Ile Gln Ala Trp Thr Lys Phe Asp Phe 
    130                 135                 140                 


Pro Gly Arg Gly Asn Thr Tyr Ser Ser Phe Lys Trp Arg Trp Tyr His 
145                 150                 155                 160 


Phe Asp Gly Val Asp Trp Asp Glu Ser Arg Lys Leu Ser Arg Ile Tyr 
                165                 170                 175     


Lys Phe Arg Gly Ile Gly Lys Ala Trp Asp Trp Glu Val Asp Thr Glu 
            180                 185                 190         


Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Leu Asp Met Asp His 
        195                 200                 205             


Pro Glu Val Val Thr Glu Leu Lys Asn Trp Gly Lys Trp Tyr Val Asn 
    210                 215                 220                 


Thr Thr Asn Ile Asp Gly Phe Arg Leu Asp Ala Val Lys His Ile Lys 
225                 230                 235                 240 


Phe Ser Phe Phe Pro Asp Trp Leu Ser Tyr Val Arg Ser Gln Thr Gly 
                245                 250                 255     


Lys Pro Leu Phe Thr Val Gly Glu Tyr Trp Ser Tyr Asp Ile Asn Lys 
            260                 265                 270         


Leu His Asn Tyr Ile Thr Lys Thr Asn Gly Thr Met Ser Leu Phe Asp 
        275                 280                 285             


Ala Pro Leu His Asn Lys Phe Tyr Thr Ala Ser Lys Ser Gly Gly Ala 
    290                 295                 300                 


Phe Asp Met Arg Thr Leu Met Thr Asn Thr Leu Met Lys Asp Gln Pro 
305                 310                 315                 320 


Thr Leu Ala Val Thr Phe Val Asp Asn His Asp Thr Glu Pro Gly Gln 
                325                 330                 335     


Ala Leu Gln Ser Trp Val Asp Pro Trp Phe Lys Pro Leu Ala Tyr Ala 
            340                 345                 350         


Phe Ile Leu Thr Arg Gln Glu Gly Tyr Pro Cys Val Phe Tyr Gly Asp 
        355                 360                 365             


Tyr Tyr Gly Ile Pro Gln Tyr Asn Ile Pro Ser Leu Lys Ser Lys Ile 
    370                 375                 380                 


Asp Pro Leu Leu Ile Ala Arg Arg Asp Tyr Ala Tyr Gly Thr Gln His 
385                 390                 395                 400 


Asp Tyr Leu Asp His Ser Asp Ile Ile Gly Trp Thr Arg Glu Gly Val 
                405                 410                 415     


Thr Glu Lys Pro Gly Ser Gly Leu Ala Ala Leu Ile Thr Asp Gly Pro 
            420                 425                 430         


Gly Gly Ser Lys Trp Met Tyr Val Gly Lys Gln His Ala Gly Lys Val 
        435                 440                 445             


Phe Tyr Asp Leu Thr Gly Asn Arg Ser Asp Thr Val Thr Ile Asn Ser 
    450                 455                 460                 


Asp Gly Trp Gly Glu Phe Lys Val Asn Gly Gly Ser Val Ser Val Trp 
465                 470                 475                 480 


Val Pro Arg Lys Thr Thr Val Ser Thr Ile Ala Arg Pro Ile Thr Thr 
                485                 490                 495     


Arg Pro Trp Thr Gly Glu Phe Val Arg Trp Thr Glu Pro Arg Leu Val 
            500                 505                 510         


Ala Trp Pro 
        515 


<210>  2
<211>  1068
<212>  DNA
<213>  Thermoascus aurantiacus


<220>
<221>  CDS
<222>  (1)..(1065)

<220>
<221>  misc_signal
<222>  (1)..(57)

<220>
<221>  misc_feature
<222>  (58)..(534)

<220>
<221>  mat_peptide
<222>  (535)..(1068)

<400>  2
atg cgg ctc gtt  gct tcc cta acg gcc  ttg gtg gcc ttg tcc  gta          45
Met Arg Leu Val  Ala Ser Leu Thr Ala  Leu Val Ala Leu Ser  Val            
            -175                 -170                 -165                

cct gtc ttt ccc  gct gct gtc aac gtg  aag cgt gct tcg tcc  tac          90
Pro Val Phe Pro  Ala Ala Val Asn Val  Lys Arg Ala Ser Ser  Tyr            
            -160                 -155                 -150                

ctg gag atc act  ctg agc cag gtc agc  aac act ctg atc aag  gcc         135
Leu Glu Ile Thr  Leu Ser Gln Val Ser  Asn Thr Leu Ile Lys  Ala            
            -145                 -140                 -135                

gtg gtc cag aac  act ggt agc gac gag  ttg tcc ttc gtt cac  ctg         180
Val Val Gln Asn  Thr Gly Ser Asp Glu  Leu Ser Phe Val His  Leu            
            -130                 -125                 -120                

aac ttc ttc aag  gac ccc gct cct gtc  aaa aag gta tcg gtc  tat         225
Asn Phe Phe Lys  Asp Pro Ala Pro Val  Lys Lys Val Ser Val  Tyr            
            -115                 -110                 -105                

cgc gat ggg tct  gaa gtg cag ttc gag ggc att ttg agc cgc tac aaa       273
Arg Asp Gly Ser  Glu Val Gln Phe Glu Gly Ile Leu Ser Arg Tyr Lys          
            -100                 -95                 -90                  

tcg act ggc ctc tct cgt gac gcc ttt act tat ctg gct ccc gga gag        321
Ser Thr Gly Leu Ser Arg Asp Ala Phe Thr Tyr Leu Ala Pro Gly Glu           
        -85                 -80                 -75                       

tcc gtc gag gac gtt ttt gat att gct tcg act tac gat ctg acc agc        369
Ser Val Glu Asp Val Phe Asp Ile Ala Ser Thr Tyr Asp Leu Thr Ser           
    -70                 -65                 -60                           

ggc ggc cct gta act atc cgt act gag gga gtt gtt ccc tac gcc acg        417
Gly Gly Pro Val Thr Ile Arg Thr Glu Gly Val Val Pro Tyr Ala Thr           
-55                 -50                 -45                 -40           

gct aac agc act gat att gcc ggc tac atc tca tac tcg tct aat gtg        465
Ala Asn Ser Thr Asp Ile Ala Gly Tyr Ile Ser Tyr Ser Ser Asn Val           
                -35                 -30                 -25               

ttg acc att gat gtc gat ggc gcc gct gct gcc act gtc tcc aag gca        513
Leu Thr Ile Asp Val Asp Gly Ala Ala Ala Ala Thr Val Ser Lys Ala           
            -20                 -15                 -10                   

atc act cct ttg gac cgc cgc act agg atc agt tcc tgc tcc ggc agc        561
Ile Thr Pro Leu Asp Arg Arg Thr Arg Ile Ser Ser Cys Ser Gly Ser           
        -5              -1  1               5                             

aga cag agc gct ctt act acg gct ctc aga aac gct gct tct ctt gcc        609
Arg Gln Ser Ala Leu Thr Thr Ala Leu Arg Asn Ala Ala Ser Leu Ala           
10                  15                  20                  25            

aac gca gct gcc gac gcg gct cag tct gga tca gct tca aag ttc agc        657
Asn Ala Ala Ala Asp Ala Ala Gln Ser Gly Ser Ala Ser Lys Phe Ser           
                30                  35                  40                

gag tac ttc aag act act tct agc tct acc cgc cag acc gtg gct gcg        705
Glu Tyr Phe Lys Thr Thr Ser Ser Ser Thr Arg Gln Thr Val Ala Ala           
            45                  50                  55                    

cgt ctt cgg gct gtt gcg cgg gag gca tct tcg tct tct tcg gga gcc        753
Arg Leu Arg Ala Val Ala Arg Glu Ala Ser Ser Ser Ser Ser Gly Ala           
        60                  65                  70                        

acc acg tac tac tgc gac gat ccc tac ggc tac tgt tcc tcc aac gtc        801
Thr Thr Tyr Tyr Cys Asp Asp Pro Tyr Gly Tyr Cys Ser Ser Asn Val           
    75                  80                  85                            

ctg gct tac acc ctg cct tca tac aac ata atc gcc aac tgt gac att        849
Leu Ala Tyr Thr Leu Pro Ser Tyr Asn Ile Ile Ala Asn Cys Asp Ile           
90                  95                  100                 105           

ttc tat act tac ctg ccg gct ctg acc agt acc tgt cac gct cag gat        897
Phe Tyr Thr Tyr Leu Pro Ala Leu Thr Ser Thr Cys His Ala Gln Asp           
                110                 115                 120               

caa gcg acc act gcc ctt cac gag ttc acc cat gcg cct ggc gtc tac        945
Gln Ala Thr Thr Ala Leu His Glu Phe Thr His Ala Pro Gly Val Tyr           
            125                 130                 135                   

agc cct ggc acg gac gac ctg gcg tat ggc tac cag gct gcg atg ggt        993
Ser Pro Gly Thr Asp Asp Leu Ala Tyr Gly Tyr Gln Ala Ala Met Gly           
        140                 145                 150                       

ctc agc agc agc cag gct gtc atg aac gct gac acc tac gct ctc tat       1041
Leu Ser Ser Ser Gln Ala Val Met Asn Ala Asp Thr Tyr Ala Leu Tyr           
    155                 160                 165                           

gcg aat gcc ata tac ctt ggt tgc taa                                   1068
Ala Asn Ala Ile Tyr Leu Gly Cys                                           
170                 175                                                   


<210>  3
<211>  355
<212>  PRT
<213>  Thermoascus aurantiacus

<400>  3

Met Arg Leu Val  Ala Ser Leu Thr Ala  Leu Val Ala Leu Ser  Val 
            -175                 -170                 -165     


Pro Val Phe Pro  Ala Ala Val Asn Val  Lys Arg Ala Ser Ser  Tyr 
            -160                 -155                 -150     


Leu Glu Ile Thr  Leu Ser Gln Val Ser  Asn Thr Leu Ile Lys  Ala 
            -145                 -140                 -135     


Val Val Gln Asn  Thr Gly Ser Asp Glu  Leu Ser Phe Val His  Leu 
            -130                 -125                 -120     


Asn Phe Phe Lys  Asp Pro Ala Pro Val  Lys Lys Val Ser Val  Tyr 
            -115                 -110                 -105     


Arg Asp Gly Ser  Glu Val Gln Phe Glu Gly Ile Leu Ser Arg Tyr Lys 
            -100                 -95                 -90         


Ser Thr Gly Leu Ser Arg Asp Ala Phe Thr Tyr Leu Ala Pro Gly Glu 
        -85                 -80                 -75             


Ser Val Glu Asp Val Phe Asp Ile Ala Ser Thr Tyr Asp Leu Thr Ser 
    -70                 -65                 -60                 


Gly Gly Pro Val Thr Ile Arg Thr Glu Gly Val Val Pro Tyr Ala Thr 
-55                 -50                 -45                 -40 


Ala Asn Ser Thr Asp Ile Ala Gly Tyr Ile Ser Tyr Ser Ser Asn Val 
                -35                 -30                 -25     


Leu Thr Ile Asp Val Asp Gly Ala Ala Ala Ala Thr Val Ser Lys Ala 
            -20                 -15                 -10         


Ile Thr Pro Leu Asp Arg Arg Thr Arg Ile Ser Ser Cys Ser Gly Ser 
        -5              -1  1               5                   


Arg Gln Ser Ala Leu Thr Thr Ala Leu Arg Asn Ala Ala Ser Leu Ala 
10                  15                  20                  25  


Asn Ala Ala Ala Asp Ala Ala Gln Ser Gly Ser Ala Ser Lys Phe Ser 
                30                  35                  40      


Glu Tyr Phe Lys Thr Thr Ser Ser Ser Thr Arg Gln Thr Val Ala Ala 
            45                  50                  55          


Arg Leu Arg Ala Val Ala Arg Glu Ala Ser Ser Ser Ser Ser Gly Ala 
        60                  65                  70              


Thr Thr Tyr Tyr Cys Asp Asp Pro Tyr Gly Tyr Cys Ser Ser Asn Val 
    75                  80                  85                  


Leu Ala Tyr Thr Leu Pro Ser Tyr Asn Ile Ile Ala Asn Cys Asp Ile 
90                  95                  100                 105 


Phe Tyr Thr Tyr Leu Pro Ala Leu Thr Ser Thr Cys His Ala Gln Asp 
                110                 115                 120     


Gln Ala Thr Thr Ala Leu His Glu Phe Thr His Ala Pro Gly Val Tyr 
            125                 130                 135         


Ser Pro Gly Thr Asp Asp Leu Ala Tyr Gly Tyr Gln Ala Ala Met Gly 
        140                 145                 150             


Leu Ser Ser Ser Gln Ala Val Met Asn Ala Asp Thr Tyr Ala Leu Tyr 
    155                 160                 165                 


Ala Asn Ala Ile Tyr Leu Gly Cys 
170                 175         


<210>  4
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  4
aacgacggta cccggggatc ggatccatgc ggctcgttgc ttccctaac                   49


<210>  5
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial Construct

<400>  5
ctaattacat gatgcggccc ttaattaatt agcaaccaag gtatatgg                    48


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial Construct

<400>  6
taggagttta gtgaacttgc                                                   20


<210>  7
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial Construct

<400>  7
ttcgagcgtc ccaaaacc                                                     18


<210>  8
<211>  1851
<212>  DNA
<213>  Penicillium oxalicum


<220>
<221>  CDS
<222>  (1)..(1851)

<400>  8
atg cgt ctc act cta tta tca ggt gta gcc ggc gtt ctc tgc gca gga         48
Met Arg Leu Thr Leu Leu Ser Gly Val Ala Gly Val Leu Cys Ala Gly           
1               5                   10                  15                

cag ctg acg gcg gcg cgt cct gat ccc aag ggt ggg aat ctg acg ccg         96
Gln Leu Thr Ala Ala Arg Pro Asp Pro Lys Gly Gly Asn Leu Thr Pro           
            20                  25                  30                    

ttc atc cac aaa gag ggc gag cgg tcg ctc caa ggc atc ttg gac aat        144
Phe Ile His Lys Glu Gly Glu Arg Ser Leu Gln Gly Ile Leu Asp Asn           
        35                  40                  45                        

ctc ggt ggg cga ggt aag aaa aca ccc ggc act gcc gca ggg ttg ttt        192
Leu Gly Gly Arg Gly Lys Lys Thr Pro Gly Thr Ala Ala Gly Leu Phe           
    50                  55                  60                            

att gcc agt cca aac aca gag aat cca aac tat tat tat aca tgg act        240
Ile Ala Ser Pro Asn Thr Glu Asn Pro Asn Tyr Tyr Tyr Thr Trp Thr           
65                  70                  75                  80            

cgt gac tca gct ttg act gcc aag tgc ttg atc gac ctg ttc gaa gac        288
Arg Asp Ser Ala Leu Thr Ala Lys Cys Leu Ile Asp Leu Phe Glu Asp           
                85                  90                  95                

tct cgg gca aag ttt cca att gac cgc aaa tac ttg gaa aca gga att        336
Ser Arg Ala Lys Phe Pro Ile Asp Arg Lys Tyr Leu Glu Thr Gly Ile           
            100                 105                 110                   

cgg gac tac gtg tcg tcc caa gca atc ctc cag agt gtg tct aat cct        384
Arg Asp Tyr Val Ser Ser Gln Ala Ile Leu Gln Ser Val Ser Asn Pro           
        115                 120                 125                       

tct gga acc ctg aag gat ggc tct ggt ctg ggt gaa ccc aag ttt gag        432
Ser Gly Thr Leu Lys Asp Gly Ser Gly Leu Gly Glu Pro Lys Phe Glu           
    130                 135                 140                           

att gac ctg aat ccc ttt tcg ggt gcc tgg ggt cgg cct cag cgg gat        480
Ile Asp Leu Asn Pro Phe Ser Gly Ala Trp Gly Arg Pro Gln Arg Asp           
145                 150                 155                 160           

ggc cca gcg ctg cga gcg acc gct atg atc acc tac gcc aac tac ctg        528
Gly Pro Ala Leu Arg Ala Thr Ala Met Ile Thr Tyr Ala Asn Tyr Leu           
                165                 170                 175               

ata tcc cat ggt cag aaa tcg gat gtg tca cag gtc atg tgg ccg att        576
Ile Ser His Gly Gln Lys Ser Asp Val Ser Gln Val Met Trp Pro Ile           
            180                 185                 190                   

att gcc aat gat cta gca tat gtt ggt caa tac tgg aat aat acc gga        624
Ile Ala Asn Asp Leu Ala Tyr Val Gly Gln Tyr Trp Asn Asn Thr Gly           
        195                 200                 205                       

ttt gac ctg tgg gaa gag gtg gat ggg tca agc ttt ttc acg att gcg        672
Phe Asp Leu Trp Glu Glu Val Asp Gly Ser Ser Phe Phe Thr Ile Ala           
    210                 215                 220                           

gtc cag cac cga gcc ctt gtt gaa ggc tcg caa ctg gcg aaa aag ctc        720
Val Gln His Arg Ala Leu Val Glu Gly Ser Gln Leu Ala Lys Lys Leu           
225                 230                 235                 240           

ggc aag tcc tgc gat gcc tgt gat tct cag cct ccc cag ata ttg tgt        768
Gly Lys Ser Cys Asp Ala Cys Asp Ser Gln Pro Pro Gln Ile Leu Cys           
                245                 250                 255               

ttc ctg cag agt ttc tgg aac gga aag tac atc acc tcc aac atc aac        816
Phe Leu Gln Ser Phe Trp Asn Gly Lys Tyr Ile Thr Ser Asn Ile Asn           
            260                 265                 270                   

acg caa gca agc cgc tct ggt atc gac ctg gac tct gtc ctg gga agc        864
Thr Gln Ala Ser Arg Ser Gly Ile Asp Leu Asp Ser Val Leu Gly Ser           
        275                 280                 285                       

att cat acc ttt gat ccc gaa gca gcc tgt gac gat gca act ttc cag        912
Ile His Thr Phe Asp Pro Glu Ala Ala Cys Asp Asp Ala Thr Phe Gln           
    290                 295                 300                           

cct tgt tct gcc cgc gct ctg gcg aac cac aag gtc tat gtg gat tcc        960
Pro Cys Ser Ala Arg Ala Leu Ala Asn His Lys Val Tyr Val Asp Ser           
305                 310                 315                 320           

ttc cgc tct atc tac aag att aat gcg ggt ctt gca gag gga tcg gct       1008
Phe Arg Ser Ile Tyr Lys Ile Asn Ala Gly Leu Ala Glu Gly Ser Ala           
                325                 330                 335               

gcc aac gtt ggc cgc tac ccc gag gat gtt tac caa gga ggc aat cca       1056
Ala Asn Val Gly Arg Tyr Pro Glu Asp Val Tyr Gln Gly Gly Asn Pro           
            340                 345                 350                   

tgg tat ctc gcc acc cta ggc gca tct gaa ttg ctt tac gac gcc ttg       1104
Trp Tyr Leu Ala Thr Leu Gly Ala Ser Glu Leu Leu Tyr Asp Ala Leu           
        355                 360                 365                       

tac cag tgg gac aga ctt ggc aaa ctt gaa gtc tcg gag acc tcg ttg       1152
Tyr Gln Trp Asp Arg Leu Gly Lys Leu Glu Val Ser Glu Thr Ser Leu           
    370                 375                 380                           

tca ttc ttc aaa gac ttt gac gcg acc gtg aaa att ggc tcg tac tcg       1200
Ser Phe Phe Lys Asp Phe Asp Ala Thr Val Lys Ile Gly Ser Tyr Ser           
385                 390                 395                 400           

agg aac agc aag acc tac aag aaa ttg acc cag tcc atc aag tcg tac       1248
Arg Asn Ser Lys Thr Tyr Lys Lys Leu Thr Gln Ser Ile Lys Ser Tyr           
                405                 410                 415               

gcg gac ggg ttc atc cag tta gtg cag cag tac act cct tct aat gga       1296
Ala Asp Gly Phe Ile Gln Leu Val Gln Gln Tyr Thr Pro Ser Asn Gly           
            420                 425                 430                   

tct ctg gcc gag caa tac gat cgc aat acg gct gct cct ctc tct gca       1344
Ser Leu Ala Glu Gln Tyr Asp Arg Asn Thr Ala Ala Pro Leu Ser Ala           
        435                 440                 445                       

aac gat ctg act tgg tca ttt gcc tct ttc ttg acg gct acg caa cgc       1392
Asn Asp Leu Thr Trp Ser Phe Ala Ser Phe Leu Thr Ala Thr Gln Arg           
    450                 455                 460                           

cgc gat gcc gtg gtt cct ccc tcc tgg ggc gca aag tcg gca aac aaa       1440
Arg Asp Ala Val Val Pro Pro Ser Trp Gly Ala Lys Ser Ala Asn Lys           
465                 470                 475                 480           

gtc cca acc act tgt tca gcc tcc cct gtt gtg ggt act tat aag gcg       1488
Val Pro Thr Thr Cys Ser Ala Ser Pro Val Val Gly Thr Tyr Lys Ala           
                485                 490                 495               

ccc acg gca act ttc tca tcc aag act aag tgc gtc ccc gct aaa gat       1536
Pro Thr Ala Thr Phe Ser Ser Lys Thr Lys Cys Val Pro Ala Lys Asp           
            500                 505                 510                   

att gtg cct atc acg ttc tac ctg att gag aac act tac tat gga gag       1584
Ile Val Pro Ile Thr Phe Tyr Leu Ile Glu Asn Thr Tyr Tyr Gly Glu           
        515                 520                 525                       

aac gtc ttc atg agt ggc aac att act gcg ctg ggt aac tgg gac gcc       1632
Asn Val Phe Met Ser Gly Asn Ile Thr Ala Leu Gly Asn Trp Asp Ala           
    530                 535                 540                           

aag aaa ggc ttc cca ctc acc gca aac ctc tac acg caa gat caa aac       1680
Lys Lys Gly Phe Pro Leu Thr Ala Asn Leu Tyr Thr Gln Asp Gln Asn           
545                 550                 555                 560           

ttg tgg ttc gcc agt gtc gag ttc atc cca gca ggc aca ccc ttt gag       1728
Leu Trp Phe Ala Ser Val Glu Phe Ile Pro Ala Gly Thr Pro Phe Glu           
                565                 570                 575               

tac aag tac tac aag gtc gag ccc aat ggc gat att act tgg gag aag       1776
Tyr Lys Tyr Tyr Lys Val Glu Pro Asn Gly Asp Ile Thr Trp Glu Lys           
            580                 585                 590                   

ggt ccc aac cgg gtg ttc gtc gct ccc acg gga tgc cca gtt cag cct       1824
Gly Pro Asn Arg Val Phe Val Ala Pro Thr Gly Cys Pro Val Gln Pro           
        595                 600                 605                       

cac tcc aac gac gtg tgg cag ttt tga                                   1851
His Ser Asn Asp Val Trp Gln Phe                                           
    610                 615                                               


<210>  9
<211>  616
<212>  PRT
<213>  Penicillium oxalicum

<400>  9

Met Arg Leu Thr Leu Leu Ser Gly Val Ala Gly Val Leu Cys Ala Gly 
1               5                   10                  15      


Gln Leu Thr Ala Ala Arg Pro Asp Pro Lys Gly Gly Asn Leu Thr Pro 
            20                  25                  30          


Phe Ile His Lys Glu Gly Glu Arg Ser Leu Gln Gly Ile Leu Asp Asn 
        35                  40                  45              


Leu Gly Gly Arg Gly Lys Lys Thr Pro Gly Thr Ala Ala Gly Leu Phe 
    50                  55                  60                  


Ile Ala Ser Pro Asn Thr Glu Asn Pro Asn Tyr Tyr Tyr Thr Trp Thr 
65                  70                  75                  80  


Arg Asp Ser Ala Leu Thr Ala Lys Cys Leu Ile Asp Leu Phe Glu Asp 
                85                  90                  95      


Ser Arg Ala Lys Phe Pro Ile Asp Arg Lys Tyr Leu Glu Thr Gly Ile 
            100                 105                 110         


Arg Asp Tyr Val Ser Ser Gln Ala Ile Leu Gln Ser Val Ser Asn Pro 
        115                 120                 125             


Ser Gly Thr Leu Lys Asp Gly Ser Gly Leu Gly Glu Pro Lys Phe Glu 
    130                 135                 140                 


Ile Asp Leu Asn Pro Phe Ser Gly Ala Trp Gly Arg Pro Gln Arg Asp 
145                 150                 155                 160 


Gly Pro Ala Leu Arg Ala Thr Ala Met Ile Thr Tyr Ala Asn Tyr Leu 
                165                 170                 175     


Ile Ser His Gly Gln Lys Ser Asp Val Ser Gln Val Met Trp Pro Ile 
            180                 185                 190         


Ile Ala Asn Asp Leu Ala Tyr Val Gly Gln Tyr Trp Asn Asn Thr Gly 
        195                 200                 205             


Phe Asp Leu Trp Glu Glu Val Asp Gly Ser Ser Phe Phe Thr Ile Ala 
    210                 215                 220                 


Val Gln His Arg Ala Leu Val Glu Gly Ser Gln Leu Ala Lys Lys Leu 
225                 230                 235                 240 


Gly Lys Ser Cys Asp Ala Cys Asp Ser Gln Pro Pro Gln Ile Leu Cys 
                245                 250                 255     


Phe Leu Gln Ser Phe Trp Asn Gly Lys Tyr Ile Thr Ser Asn Ile Asn 
            260                 265                 270         


Thr Gln Ala Ser Arg Ser Gly Ile Asp Leu Asp Ser Val Leu Gly Ser 
        275                 280                 285             


Ile His Thr Phe Asp Pro Glu Ala Ala Cys Asp Asp Ala Thr Phe Gln 
    290                 295                 300                 


Pro Cys Ser Ala Arg Ala Leu Ala Asn His Lys Val Tyr Val Asp Ser 
305                 310                 315                 320 


Phe Arg Ser Ile Tyr Lys Ile Asn Ala Gly Leu Ala Glu Gly Ser Ala 
                325                 330                 335     


Ala Asn Val Gly Arg Tyr Pro Glu Asp Val Tyr Gln Gly Gly Asn Pro 
            340                 345                 350         


Trp Tyr Leu Ala Thr Leu Gly Ala Ser Glu Leu Leu Tyr Asp Ala Leu 
        355                 360                 365             


Tyr Gln Trp Asp Arg Leu Gly Lys Leu Glu Val Ser Glu Thr Ser Leu 
    370                 375                 380                 


Ser Phe Phe Lys Asp Phe Asp Ala Thr Val Lys Ile Gly Ser Tyr Ser 
385                 390                 395                 400 


Arg Asn Ser Lys Thr Tyr Lys Lys Leu Thr Gln Ser Ile Lys Ser Tyr 
                405                 410                 415     


Ala Asp Gly Phe Ile Gln Leu Val Gln Gln Tyr Thr Pro Ser Asn Gly 
            420                 425                 430         


Ser Leu Ala Glu Gln Tyr Asp Arg Asn Thr Ala Ala Pro Leu Ser Ala 
        435                 440                 445             


Asn Asp Leu Thr Trp Ser Phe Ala Ser Phe Leu Thr Ala Thr Gln Arg 
    450                 455                 460                 


Arg Asp Ala Val Val Pro Pro Ser Trp Gly Ala Lys Ser Ala Asn Lys 
465                 470                 475                 480 


Val Pro Thr Thr Cys Ser Ala Ser Pro Val Val Gly Thr Tyr Lys Ala 
                485                 490                 495     


Pro Thr Ala Thr Phe Ser Ser Lys Thr Lys Cys Val Pro Ala Lys Asp 
            500                 505                 510         


Ile Val Pro Ile Thr Phe Tyr Leu Ile Glu Asn Thr Tyr Tyr Gly Glu 
        515                 520                 525             


Asn Val Phe Met Ser Gly Asn Ile Thr Ala Leu Gly Asn Trp Asp Ala 
    530                 535                 540                 


Lys Lys Gly Phe Pro Leu Thr Ala Asn Leu Tyr Thr Gln Asp Gln Asn 
545                 550                 555                 560 


Leu Trp Phe Ala Ser Val Glu Phe Ile Pro Ala Gly Thr Pro Phe Glu 
                565                 570                 575     


Tyr Lys Tyr Tyr Lys Val Glu Pro Asn Gly Asp Ile Thr Trp Glu Lys 
            580                 585                 590         


Gly Pro Asn Arg Val Phe Val Ala Pro Thr Gly Cys Pro Val Gln Pro 
        595                 600                 605             


His Ser Asn Asp Val Trp Gln Phe 
    610                 615     


<210>  10
<211>  4014
<212>  DNA
<213>  Thermococcus hydrothermalis


<220>
<221>  CDS
<222>  (1)..(4011)

<220>
<221>  misc_signal
<222>  (1)..(81)

<220>
<221>  mat_peptide
<222>  (82)..(4014)

<400>  10
atg agg cgg gtg gtt gcc ctc ttc att gca att ttg atg ctt gga agc         48
Met Arg Arg Val Val Ala Leu Phe Ile Ala Ile Leu Met Leu Gly Ser           
        -25                 -20                 -15                       

atc gtt gga gcg aac gtt aag agc gtt ggc gcg gcg gag ccg aag ccg         96
Ile Val Gly Ala Asn Val Lys Ser Val Gly Ala Ala Glu Pro Lys Pro           
    -10                 -5              -1  1               5             

ctc aac gtc ata ata gtc tgg cac cag cac cag ccc tac tac tac gac        144
Leu Asn Val Ile Ile Val Trp His Gln His Gln Pro Tyr Tyr Tyr Asp           
                10                  15                  20                

cct gtc cag gac gtc tac acc agg ccc tgg gtc agg ctc cac gcg gcg        192
Pro Val Gln Asp Val Tyr Thr Arg Pro Trp Val Arg Leu His Ala Ala           
            25                  30                  35                    

aac aac tac tgg aag atg gcc cac tac ctg agc cag tac ccg gag gtt        240
Asn Asn Tyr Trp Lys Met Ala His Tyr Leu Ser Gln Tyr Pro Glu Val           
        40                  45                  50                        

cac gcc acc att gac ctc tcg ggt tcg ctg ata gcc cag ctt gcc gac        288
His Ala Thr Ile Asp Leu Ser Gly Ser Leu Ile Ala Gln Leu Ala Asp           
    55                  60                  65                            

tac atg aac ggc aag aag gac acc tac cag ata atc acc gag aag ata        336
Tyr Met Asn Gly Lys Lys Asp Thr Tyr Gln Ile Ile Thr Glu Lys Ile           
70                  75                  80                  85            

gcc aac ggg gaa ccc ctc acc gtc gac gag aag tgg ttc atg ctc cag        384
Ala Asn Gly Glu Pro Leu Thr Val Asp Glu Lys Trp Phe Met Leu Gln           
                90                  95                  100               

gca ccg gga ggg ttc ttc gac aac acc atc ccc tgg aac ggt gaa ccg        432
Ala Pro Gly Gly Phe Phe Asp Asn Thr Ile Pro Trp Asn Gly Glu Pro           
            105                 110                 115                   

ata acc gac ccc aac ggc aac ccg ata agg gac ttc tgg gac cgc tac        480
Ile Thr Asp Pro Asn Gly Asn Pro Ile Arg Asp Phe Trp Asp Arg Tyr           
        120                 125                 130                       

acg gag ctg aag aac aag atg ctc agc gca aag gcc aag tac gca aac        528
Thr Glu Leu Lys Asn Lys Met Leu Ser Ala Lys Ala Lys Tyr Ala Asn           
    135                 140                 145                           

ttc gtg act gag agc cag aag gtc gct gtg acg aac gag ttc aca gag        576
Phe Val Thr Glu Ser Gln Lys Val Ala Val Thr Asn Glu Phe Thr Glu           
150                 155                 160                 165           

cag gac tac ata gac cta gcg gtt ctc ttc aat ctc gct tgg att gac        624
Gln Asp Tyr Ile Asp Leu Ala Val Leu Phe Asn Leu Ala Trp Ile Asp           
                170                 175                 180               

tac aat tac atc acg agc acg ccg gag ttc aag gcc ctc tac gac aag        672
Tyr Asn Tyr Ile Thr Ser Thr Pro Glu Phe Lys Ala Leu Tyr Asp Lys           
            185                 190                 195                   

gtt gac gag ggc ggc tat aca agg gcg gac gtc aaa acc gtt ctc gac        720
Val Asp Glu Gly Gly Tyr Thr Arg Ala Asp Val Lys Thr Val Leu Asp           
        200                 205                 210                       

gcc cag atc tgg ctt ctc aac cac acc ttc gag gag cac gag aag ata        768
Ala Gln Ile Trp Leu Leu Asn His Thr Phe Glu Glu His Glu Lys Ile           
    215                 220                 225                           

aac ctc ctc ctc gga aac ggc aac gtc gag gtc acg gtc gtt ccc tac        816
Asn Leu Leu Leu Gly Asn Gly Asn Val Glu Val Thr Val Val Pro Tyr           
230                 235                 240                 245           

gcc cac ccg ata ggc ccg ata ctc aac gac ttc ggc tgg gac agc gac        864
Ala His Pro Ile Gly Pro Ile Leu Asn Asp Phe Gly Trp Asp Ser Asp           
                250                 255                 260               

ttc aac gac cag gtc aag aag gcc gac gaa ctg tac aag ccg tac ctc        912
Phe Asn Asp Gln Val Lys Lys Ala Asp Glu Leu Tyr Lys Pro Tyr Leu           
            265                 270                 275                   

ggc ggc ggc acc gcg gtt cca aaa ggc gga tgg gcg gct gag agc gcc        960
Gly Gly Gly Thr Ala Val Pro Lys Gly Gly Trp Ala Ala Glu Ser Ala           
        280                 285                 290                       

ctc aac gac aaa act ctg gag atc ctc gcc gag aac ggc tgg gag tgg       1008
Leu Asn Asp Lys Thr Leu Glu Ile Leu Ala Glu Asn Gly Trp Glu Trp           
    295                 300                 305                           

gtc atg acc gac cag atg gtt ctc gga aag ctc ggc att gag gga acc       1056
Val Met Thr Asp Gln Met Val Leu Gly Lys Leu Gly Ile Glu Gly Thr           
310                 315                 320                 325           

gtc gag aac tac cac aag ccc tgg gtg gcc gag ttc aac gga aag aag       1104
Val Glu Asn Tyr His Lys Pro Trp Val Ala Glu Phe Asn Gly Lys Lys           
                330                 335                 340               

ata tac ctc ttc cca aga aat cac gat cta agt gac aga gtt ggc ttt       1152
Ile Tyr Leu Phe Pro Arg Asn His Asp Leu Ser Asp Arg Val Gly Phe           
            345                 350                 355                   

acc tac agc gga atg aac cag cag cag gcc gtt gag gac ttc gtc aac       1200
Thr Tyr Ser Gly Met Asn Gln Gln Gln Ala Val Glu Asp Phe Val Asn           
        360                 365                 370                       

gag ctc ctc aag ctc cag aag cag aac tac gat ggc tcg ctg gtt tac       1248
Glu Leu Leu Lys Leu Gln Lys Gln Asn Tyr Asp Gly Ser Leu Val Tyr           
    375                 380                 385                           

gtg gtc acg ctc gac ggc gag aac ccc gtg gag aac tac ccc tac gac       1296
Val Val Thr Leu Asp Gly Glu Asn Pro Val Glu Asn Tyr Pro Tyr Asp           
390                 395                 400                 405           

ggg gag ctc ttc ctc acc gaa ctc tac aag aag ctg acc gaa ctc cag       1344
Gly Glu Leu Phe Leu Thr Glu Leu Tyr Lys Lys Leu Thr Glu Leu Gln           
                410                 415                 420               

gag cag ggt ctc ata aga acc ctc acc ccg agc gag tac atc cag ctc       1392
Glu Gln Gly Leu Ile Arg Thr Leu Thr Pro Ser Glu Tyr Ile Gln Leu           
            425                 430                 435                   

tac ggc gac aag gcc aac aag ctc aca cct cgg atg atg gag cgc ctt       1440
Tyr Gly Asp Lys Ala Asn Lys Leu Thr Pro Arg Met Met Glu Arg Leu           
        440                 445                 450                       

gac ctc acc gga gac aac gtt aac gcc ctc ctc aag gcc cag agc ctc       1488
Asp Leu Thr Gly Asp Asn Val Asn Ala Leu Leu Lys Ala Gln Ser Leu           
    455                 460                 465                           

ggc gaa ctc tac gac atg acc ggc gtt aag gag gag atg cag tgg ccc       1536
Gly Glu Leu Tyr Asp Met Thr Gly Val Lys Glu Glu Met Gln Trp Pro           
470                 475                 480                 485           

gag agc agc tgg ata gac gga acc ctc tcc acg tgg ata ggc gag ccc       1584
Glu Ser Ser Trp Ile Asp Gly Thr Leu Ser Thr Trp Ile Gly Glu Pro           
                490                 495                 500               

cag gag aac tac ggc tgg tac tgg ctc tac atg gcc agg aag gcc ctt       1632
Gln Glu Asn Tyr Gly Trp Tyr Trp Leu Tyr Met Ala Arg Lys Ala Leu           
            505                 510                 515                   

atg gag aac aag gat aaa atg agc cag gcg gac tgg gag aag gcc tac       1680
Met Glu Asn Lys Asp Lys Met Ser Gln Ala Asp Trp Glu Lys Ala Tyr           
        520                 525                 530                       

gag tac ctg ctc cgc gcc gag gca agc gac tgg ttc tgg tgg tac gga       1728
Glu Tyr Leu Leu Arg Ala Glu Ala Ser Asp Trp Phe Trp Trp Tyr Gly           
    535                 540                 545                           

agc gac cag gac agc ggc cag gac tac acc ttc gac cgc tac ctg aag       1776
Ser Asp Gln Asp Ser Gly Gln Asp Tyr Thr Phe Asp Arg Tyr Leu Lys           
550                 555                 560                 565           

acc tac ctc tac gag atg tac aag ctg gca gga gtc gag ccg ccg agc       1824
Thr Tyr Leu Tyr Glu Met Tyr Lys Leu Ala Gly Val Glu Pro Pro Ser           
                570                 575                 580               

tac ctc ttc ggc aac tac ttc ccg gac gga gag ccc tac acc acg agg       1872
Tyr Leu Phe Gly Asn Tyr Phe Pro Asp Gly Glu Pro Tyr Thr Thr Arg           
            585                 590                 595                   

ggc ctg gtc gga ctc aag gac ggc gag atg aag aac ttc tcc agc atg       1920
Gly Leu Val Gly Leu Lys Asp Gly Glu Met Lys Asn Phe Ser Ser Met           
        600                 605                 610                       

tcc ccg ctg gca aag ggc gtg agc gtc tat ttc gac ggc gag ggg ata       1968
Ser Pro Leu Ala Lys Gly Val Ser Val Tyr Phe Asp Gly Glu Gly Ile           
    615                 620                 625                           

cac ttc ata gtg aaa ggg aac ctg gac agg ttc gag gtg agc atc tgg       2016
His Phe Ile Val Lys Gly Asn Leu Asp Arg Phe Glu Val Ser Ile Trp           
630                 635                 640                 645           

gag aag gat gag cgc gtt ggc aac acg ttc acc cgc ctc caa gag aag       2064
Glu Lys Asp Glu Arg Val Gly Asn Thr Phe Thr Arg Leu Gln Glu Lys           
                650                 655                 660               

ccg gac gag ttg agc tat ttc atg ttc cca ttc tca agg gac agc gtt       2112
Pro Asp Glu Leu Ser Tyr Phe Met Phe Pro Phe Ser Arg Asp Ser Val           
            665                 670                 675                   

ggt ctc ctc ata acc aag cac gtc gtg tac gag aac gga aag gcc gag       2160
Gly Leu Leu Ile Thr Lys His Val Val Tyr Glu Asn Gly Lys Ala Glu           
        680                 685                 690                       

ata tac ggc gcc acc gac tac gag aag agc gag aag ctt ggg gaa gcc       2208
Ile Tyr Gly Ala Thr Asp Tyr Glu Lys Ser Glu Lys Leu Gly Glu Ala           
    695                 700                 705                           

acc gtc aag aac acg agc gaa gga atc gaa gtc gtc ctt ccc ttt gac       2256
Thr Val Lys Asn Thr Ser Glu Gly Ile Glu Val Val Leu Pro Phe Asp           
710                 715                 720                 725           

tac ata gaa aac ccc tcc gac ttc tac ttc gct gtc tcg acg gtc aaa       2304
Tyr Ile Glu Asn Pro Ser Asp Phe Tyr Phe Ala Val Ser Thr Val Lys           
                730                 735                 740               

gat gga gac ctt gag gtg ata agc act cct gtg gag ctc aag ctc ccg       2352
Asp Gly Asp Leu Glu Val Ile Ser Thr Pro Val Glu Leu Lys Leu Pro           
            745                 750                 755                   

acc gag gtc aag gga gtc gtc ata gcc gat ata acc gac cca gaa ggc       2400
Thr Glu Val Lys Gly Val Val Ile Ala Asp Ile Thr Asp Pro Glu Gly           
        760                 765                 770                       

gac gac cat ggg ccc gga aac tac act tat ccc acg gac aag gtc ttc       2448
Asp Asp His Gly Pro Gly Asn Tyr Thr Tyr Pro Thr Asp Lys Val Phe           
    775                 780                 785                           

aag cca ggt gtt ttc gac ctc ctc cgc ttc agg atg ctc gaa cag acg       2496
Lys Pro Gly Val Phe Asp Leu Leu Arg Phe Arg Met Leu Glu Gln Thr           
790                 795                 800                 805           

gag agc tac gtc atg gag ttc tac ttc aag gac cta ggt ggt aac ccg       2544
Glu Ser Tyr Val Met Glu Phe Tyr Phe Lys Asp Leu Gly Gly Asn Pro           
                810                 815                 820               

tgg aac gga ccc aac ggc ttc agc ctc cag ata atc gag gtc tac ctc       2592
Trp Asn Gly Pro Asn Gly Phe Ser Leu Gln Ile Ile Glu Val Tyr Leu           
            825                 830                 835                   

gac ttc aag gac ggt gga aac agt tcg gcc att aag atg ttc ccc gac       2640
Asp Phe Lys Asp Gly Gly Asn Ser Ser Ala Ile Lys Met Phe Pro Asp           
        840                 845                 850                       

gga ccg gga gcc aac gtc aac ctc gac ccc gag cat cca tgg gac gtt       2688
Gly Pro Gly Ala Asn Val Asn Leu Asp Pro Glu His Pro Trp Asp Val           
    855                 860                 865                           

gcc ttc agg ata gcg ggc tgg gac tac gga aac ctc atc atc ctg ccg       2736
Ala Phe Arg Ile Ala Gly Trp Asp Tyr Gly Asn Leu Ile Ile Leu Pro           
870                 875                 880                 885           

aac gga acg gcc atc cag ggc gag atg cag att tcc gca gat ccg gtt       2784
Asn Gly Thr Ala Ile Gln Gly Glu Met Gln Ile Ser Ala Asp Pro Val           
                890                 895                 900               

aag aac gcc ata ata gtc aag gtt cca aag aag tac atc gcc ata aac       2832
Lys Asn Ala Ile Ile Val Lys Val Pro Lys Lys Tyr Ile Ala Ile Asn           
            905                 910                 915                   

gag gac tac ggc ctc tgg gga gac gtc ctc gtc ggc tcg cag gac ggc       2880
Glu Asp Tyr Gly Leu Trp Gly Asp Val Leu Val Gly Ser Gln Asp Gly           
        920                 925                 930                       

tac ggc ccg gac aag tgg aga acg gcg gca gtg gat gcg gag cag tgg       2928
Tyr Gly Pro Asp Lys Trp Arg Thr Ala Ala Val Asp Ala Glu Gln Trp           
    935                 940                 945                           

aag ctt gga ggt gcg gac ccg cag gca gtc ata aac ggc gtg gcc ccg       2976
Lys Leu Gly Gly Ala Asp Pro Gln Ala Val Ile Asn Gly Val Ala Pro           
950                 955                 960                 965           

cgc gtc att gat gag ctg gtt ccg cag ggc ttt gaa ccg acc cag gag       3024
Arg Val Ile Asp Glu Leu Val Pro Gln Gly Phe Glu Pro Thr Gln Glu           
                970                 975                 980               

gag cag ctg agc agc tac gat gca aac gac atg aag ctc gcc act gtc       3072
Glu Gln Leu Ser Ser Tyr Asp Ala Asn Asp Met Lys Leu Ala Thr Val           
            985                 990                 995                   

aag gcg ctg  cta ctc ctc aag cag  ggc atc gtt gtg acc  gac ccg        3117
Lys Ala Leu  Leu Leu Leu Lys Gln  Gly Ile Val Val Thr  Asp Pro            
        1000                 1005                 1010                    

gag gga gac  gac cac ggg ccg gga  acg tac acc tat ccg  acg gac        3162
Glu Gly Asp  Asp His Gly Pro Gly  Thr Tyr Thr Tyr Pro  Thr Asp            
        1015                 1020                 1025                    

aaa gtt ttc  aag ccc ggt gtt ttc  gac ctc ctc aag ttc  aag gtg        3207
Lys Val Phe  Lys Pro Gly Val Phe  Asp Leu Leu Lys Phe  Lys Val            
        1030                 1035                 1040                    

acc gag gga  agc gac gac tgg acg  ctg gag ttc cac ttc  aaa gac        3252
Thr Glu Gly  Ser Asp Asp Trp Thr  Leu Glu Phe His Phe  Lys Asp            
        1045                 1050                 1055                    

ctc ggt gga  aac ccg tgg aac ggg  ccg aac ggc ttc agc  ctg cag        3297
Leu Gly Gly  Asn Pro Trp Asn Gly  Pro Asn Gly Phe Ser  Leu Gln            
        1060                 1065                 1070                    

ata atc gag  gta tac ttc gac ttc  aag gag ggc ggg aac  gtc tcg        3342
Ile Ile Glu  Val Tyr Phe Asp Phe  Lys Glu Gly Gly Asn  Val Ser            
        1075                 1080                 1085                    

gcc att aag  atg ttc ccg gat ggg  ccc gga agc aac gtc  cgt ctt        3387
Ala Ile Lys  Met Phe Pro Asp Gly  Pro Gly Ser Asn Val  Arg Leu            
        1090                 1095                 1100                    

gat cca aat  cac cca tgg gac ctg  gcg ctt agg ata gcc  ggc tgg        3432
Asp Pro Asn  His Pro Trp Asp Leu  Ala Leu Arg Ile Ala  Gly Trp            
        1105                 1110                 1115                    

gac tac gga  aac ctg ata att ctg  ccc gac gga acc gcc  tac caa        3477
Asp Tyr Gly  Asn Leu Ile Ile Leu  Pro Asp Gly Thr Ala  Tyr Gln            
        1120                 1125                 1130                    

ggc gag atg  cag att tcc gca gat  ccg gtt aag aac gcc  ata ata        3522
Gly Glu Met  Gln Ile Ser Ala Asp  Pro Val Lys Asn Ala  Ile Ile            
        1135                 1140                 1145                    

gtc aag gtt  cca aag aag tac ctg  aac ata tcc gac tac  gga ctc        3567
Val Lys Val  Pro Lys Lys Tyr Leu  Asn Ile Ser Asp Tyr  Gly Leu            
        1150                 1155                 1160                    

tac acc gcc  gtc atc gtg ggt tcc  caa gac ggg tac ggc  ccg gac        3612
Tyr Thr Ala  Val Ile Val Gly Ser  Gln Asp Gly Tyr Gly  Pro Asp            
        1165                 1170                 1175                    

aag tgg agg  ccc gtg gcc gct gag  gcc gag cag tgg aag  ctc gga        3657
Lys Trp Arg  Pro Val Ala Ala Glu  Ala Glu Gln Trp Lys  Leu Gly            
        1180                 1185                 1190                    

ggc gca gac  ccc cag gcg gtc ata  gac aac ctc gta cca  agg gtc        3702
Gly Ala Asp  Pro Gln Ala Val Ile  Asp Asn Leu Val Pro  Arg Val            
        1195                 1200                 1205                    

gtt gat gaa  ctc gtg ccg gag ggc  ttc aag cca acg cag  gag gag        3747
Val Asp Glu  Leu Val Pro Glu Gly  Phe Lys Pro Thr Gln  Glu Glu            
        1210                 1215                 1220                    

cag ctg agc  agc tac gac ctt gag  aag aag acc ctg gcg  acg gtg        3792
Gln Leu Ser  Ser Tyr Asp Leu Glu  Lys Lys Thr Leu Ala  Thr Val            
        1225                 1230                 1235                    

ctc atg gta  ccg ctc gtc aat ggg  act ggc ggc gag gaa  cca acg        3837
Leu Met Val  Pro Leu Val Asn Gly  Thr Gly Gly Glu Glu  Pro Thr            
        1240                 1245                 1250                    

ccg acg gag  agc cca acg gaa acg  acg aca acc aca ccc  agc gaa        3882
Pro Thr Glu  Ser Pro Thr Glu Thr  Thr Thr Thr Thr Pro  Ser Glu            
        1255                 1260                 1265                    

aca acc acc  aca act tca acg acc  acc ggc cca agc tca  acg acc        3927
Thr Thr Thr  Thr Thr Ser Thr Thr  Thr Gly Pro Ser Ser  Thr Thr            
        1270                 1275                 1280                    

acc agc aca  ccc ggc gga gga atc  tgc ggc cca ggc att  ata gcg        3972
Thr Ser Thr  Pro Gly Gly Gly Ile  Cys Gly Pro Gly Ile  Ile Ala            
        1285                 1290                 1295                    

ggc ctg gcc  ctg ata ccg ctc ctc  ctc aag agg agg aac  tga            4014
Gly Leu Ala  Leu Ile Pro Leu Leu  Leu Lys Arg Arg Asn                     
        1300                 1305                 1310                    


<210>  11
<211>  1337
<212>  PRT
<213>  Thermococcus hydrothermalis

<400>  11

Met Arg Arg Val Val Ala Leu Phe Ile Ala Ile Leu Met Leu Gly Ser 
        -25                 -20                 -15             


Ile Val Gly Ala Asn Val Lys Ser Val Gly Ala Ala Glu Pro Lys Pro 
    -10                 -5              -1  1               5   


Leu Asn Val Ile Ile Val Trp His Gln His Gln Pro Tyr Tyr Tyr Asp 
                10                  15                  20      


Pro Val Gln Asp Val Tyr Thr Arg Pro Trp Val Arg Leu His Ala Ala 
            25                  30                  35          


Asn Asn Tyr Trp Lys Met Ala His Tyr Leu Ser Gln Tyr Pro Glu Val 
        40                  45                  50              


His Ala Thr Ile Asp Leu Ser Gly Ser Leu Ile Ala Gln Leu Ala Asp 
    55                  60                  65                  


Tyr Met Asn Gly Lys Lys Asp Thr Tyr Gln Ile Ile Thr Glu Lys Ile 
70                  75                  80                  85  


Ala Asn Gly Glu Pro Leu Thr Val Asp Glu Lys Trp Phe Met Leu Gln 
                90                  95                  100     


Ala Pro Gly Gly Phe Phe Asp Asn Thr Ile Pro Trp Asn Gly Glu Pro 
            105                 110                 115         


Ile Thr Asp Pro Asn Gly Asn Pro Ile Arg Asp Phe Trp Asp Arg Tyr 
        120                 125                 130             


Thr Glu Leu Lys Asn Lys Met Leu Ser Ala Lys Ala Lys Tyr Ala Asn 
    135                 140                 145                 


Phe Val Thr Glu Ser Gln Lys Val Ala Val Thr Asn Glu Phe Thr Glu 
150                 155                 160                 165 


Gln Asp Tyr Ile Asp Leu Ala Val Leu Phe Asn Leu Ala Trp Ile Asp 
                170                 175                 180     


Tyr Asn Tyr Ile Thr Ser Thr Pro Glu Phe Lys Ala Leu Tyr Asp Lys 
            185                 190                 195         


Val Asp Glu Gly Gly Tyr Thr Arg Ala Asp Val Lys Thr Val Leu Asp 
        200                 205                 210             


Ala Gln Ile Trp Leu Leu Asn His Thr Phe Glu Glu His Glu Lys Ile 
    215                 220                 225                 


Asn Leu Leu Leu Gly Asn Gly Asn Val Glu Val Thr Val Val Pro Tyr 
230                 235                 240                 245 


Ala His Pro Ile Gly Pro Ile Leu Asn Asp Phe Gly Trp Asp Ser Asp 
                250                 255                 260     


Phe Asn Asp Gln Val Lys Lys Ala Asp Glu Leu Tyr Lys Pro Tyr Leu 
            265                 270                 275         


Gly Gly Gly Thr Ala Val Pro Lys Gly Gly Trp Ala Ala Glu Ser Ala 
        280                 285                 290             


Leu Asn Asp Lys Thr Leu Glu Ile Leu Ala Glu Asn Gly Trp Glu Trp 
    295                 300                 305                 


Val Met Thr Asp Gln Met Val Leu Gly Lys Leu Gly Ile Glu Gly Thr 
310                 315                 320                 325 


Val Glu Asn Tyr His Lys Pro Trp Val Ala Glu Phe Asn Gly Lys Lys 
                330                 335                 340     


Ile Tyr Leu Phe Pro Arg Asn His Asp Leu Ser Asp Arg Val Gly Phe 
            345                 350                 355         


Thr Tyr Ser Gly Met Asn Gln Gln Gln Ala Val Glu Asp Phe Val Asn 
        360                 365                 370             


Glu Leu Leu Lys Leu Gln Lys Gln Asn Tyr Asp Gly Ser Leu Val Tyr 
    375                 380                 385                 


Val Val Thr Leu Asp Gly Glu Asn Pro Val Glu Asn Tyr Pro Tyr Asp 
390                 395                 400                 405 


Gly Glu Leu Phe Leu Thr Glu Leu Tyr Lys Lys Leu Thr Glu Leu Gln 
                410                 415                 420     


Glu Gln Gly Leu Ile Arg Thr Leu Thr Pro Ser Glu Tyr Ile Gln Leu 
            425                 430                 435         


Tyr Gly Asp Lys Ala Asn Lys Leu Thr Pro Arg Met Met Glu Arg Leu 
        440                 445                 450             


Asp Leu Thr Gly Asp Asn Val Asn Ala Leu Leu Lys Ala Gln Ser Leu 
    455                 460                 465                 


Gly Glu Leu Tyr Asp Met Thr Gly Val Lys Glu Glu Met Gln Trp Pro 
470                 475                 480                 485 


Glu Ser Ser Trp Ile Asp Gly Thr Leu Ser Thr Trp Ile Gly Glu Pro 
                490                 495                 500     


Gln Glu Asn Tyr Gly Trp Tyr Trp Leu Tyr Met Ala Arg Lys Ala Leu 
            505                 510                 515         


Met Glu Asn Lys Asp Lys Met Ser Gln Ala Asp Trp Glu Lys Ala Tyr 
        520                 525                 530             


Glu Tyr Leu Leu Arg Ala Glu Ala Ser Asp Trp Phe Trp Trp Tyr Gly 
    535                 540                 545                 


Ser Asp Gln Asp Ser Gly Gln Asp Tyr Thr Phe Asp Arg Tyr Leu Lys 
550                 555                 560                 565 


Thr Tyr Leu Tyr Glu Met Tyr Lys Leu Ala Gly Val Glu Pro Pro Ser 
                570                 575                 580     


Tyr Leu Phe Gly Asn Tyr Phe Pro Asp Gly Glu Pro Tyr Thr Thr Arg 
            585                 590                 595         


Gly Leu Val Gly Leu Lys Asp Gly Glu Met Lys Asn Phe Ser Ser Met 
        600                 605                 610             


Ser Pro Leu Ala Lys Gly Val Ser Val Tyr Phe Asp Gly Glu Gly Ile 
    615                 620                 625                 


His Phe Ile Val Lys Gly Asn Leu Asp Arg Phe Glu Val Ser Ile Trp 
630                 635                 640                 645 


Glu Lys Asp Glu Arg Val Gly Asn Thr Phe Thr Arg Leu Gln Glu Lys 
                650                 655                 660     


Pro Asp Glu Leu Ser Tyr Phe Met Phe Pro Phe Ser Arg Asp Ser Val 
            665                 670                 675         


Gly Leu Leu Ile Thr Lys His Val Val Tyr Glu Asn Gly Lys Ala Glu 
        680                 685                 690             


Ile Tyr Gly Ala Thr Asp Tyr Glu Lys Ser Glu Lys Leu Gly Glu Ala 
    695                 700                 705                 


Thr Val Lys Asn Thr Ser Glu Gly Ile Glu Val Val Leu Pro Phe Asp 
710                 715                 720                 725 


Tyr Ile Glu Asn Pro Ser Asp Phe Tyr Phe Ala Val Ser Thr Val Lys 
                730                 735                 740     


Asp Gly Asp Leu Glu Val Ile Ser Thr Pro Val Glu Leu Lys Leu Pro 
            745                 750                 755         


Thr Glu Val Lys Gly Val Val Ile Ala Asp Ile Thr Asp Pro Glu Gly 
        760                 765                 770             


Asp Asp His Gly Pro Gly Asn Tyr Thr Tyr Pro Thr Asp Lys Val Phe 
    775                 780                 785                 


Lys Pro Gly Val Phe Asp Leu Leu Arg Phe Arg Met Leu Glu Gln Thr 
790                 795                 800                 805 


Glu Ser Tyr Val Met Glu Phe Tyr Phe Lys Asp Leu Gly Gly Asn Pro 
                810                 815                 820     


Trp Asn Gly Pro Asn Gly Phe Ser Leu Gln Ile Ile Glu Val Tyr Leu 
            825                 830                 835         


Asp Phe Lys Asp Gly Gly Asn Ser Ser Ala Ile Lys Met Phe Pro Asp 
        840                 845                 850             


Gly Pro Gly Ala Asn Val Asn Leu Asp Pro Glu His Pro Trp Asp Val 
    855                 860                 865                 


Ala Phe Arg Ile Ala Gly Trp Asp Tyr Gly Asn Leu Ile Ile Leu Pro 
870                 875                 880                 885 


Asn Gly Thr Ala Ile Gln Gly Glu Met Gln Ile Ser Ala Asp Pro Val 
                890                 895                 900     


Lys Asn Ala Ile Ile Val Lys Val Pro Lys Lys Tyr Ile Ala Ile Asn 
            905                 910                 915         


Glu Asp Tyr Gly Leu Trp Gly Asp Val Leu Val Gly Ser Gln Asp Gly 
        920                 925                 930             


Tyr Gly Pro Asp Lys Trp Arg Thr Ala Ala Val Asp Ala Glu Gln Trp 
    935                 940                 945                 


Lys Leu Gly Gly Ala Asp Pro Gln Ala Val Ile Asn Gly Val Ala Pro 
950                 955                 960                 965 


Arg Val Ile Asp Glu Leu Val Pro Gln Gly Phe Glu Pro Thr Gln Glu 
                970                 975                 980     


Glu Gln Leu Ser Ser Tyr Asp Ala Asn Asp Met Lys Leu Ala Thr Val 
            985                 990                 995         


Lys Ala Leu  Leu Leu Leu Lys Gln  Gly Ile Val Val Thr  Asp Pro 
        1000                 1005                 1010         


Glu Gly Asp  Asp His Gly Pro Gly  Thr Tyr Thr Tyr Pro  Thr Asp 
        1015                 1020                 1025         


Lys Val Phe  Lys Pro Gly Val Phe  Asp Leu Leu Lys Phe  Lys Val 
        1030                 1035                 1040         


Thr Glu Gly  Ser Asp Asp Trp Thr  Leu Glu Phe His Phe  Lys Asp 
        1045                 1050                 1055         


Leu Gly Gly  Asn Pro Trp Asn Gly  Pro Asn Gly Phe Ser  Leu Gln 
        1060                 1065                 1070         


Ile Ile Glu  Val Tyr Phe Asp Phe  Lys Glu Gly Gly Asn  Val Ser 
        1075                 1080                 1085         


Ala Ile Lys  Met Phe Pro Asp Gly  Pro Gly Ser Asn Val  Arg Leu 
        1090                 1095                 1100         


Asp Pro Asn  His Pro Trp Asp Leu  Ala Leu Arg Ile Ala  Gly Trp 
        1105                 1110                 1115         


Asp Tyr Gly  Asn Leu Ile Ile Leu  Pro Asp Gly Thr Ala  Tyr Gln 
        1120                 1125                 1130         


Gly Glu Met  Gln Ile Ser Ala Asp  Pro Val Lys Asn Ala  Ile Ile 
        1135                 1140                 1145         


Val Lys Val  Pro Lys Lys Tyr Leu  Asn Ile Ser Asp Tyr  Gly Leu 
        1150                 1155                 1160         


Tyr Thr Ala  Val Ile Val Gly Ser  Gln Asp Gly Tyr Gly  Pro Asp 
        1165                 1170                 1175         


Lys Trp Arg  Pro Val Ala Ala Glu  Ala Glu Gln Trp Lys  Leu Gly 
        1180                 1185                 1190         


Gly Ala Asp  Pro Gln Ala Val Ile  Asp Asn Leu Val Pro  Arg Val 
        1195                 1200                 1205         


Val Asp Glu  Leu Val Pro Glu Gly  Phe Lys Pro Thr Gln  Glu Glu 
        1210                 1215                 1220         


Gln Leu Ser  Ser Tyr Asp Leu Glu  Lys Lys Thr Leu Ala  Thr Val 
        1225                 1230                 1235         


Leu Met Val  Pro Leu Val Asn Gly  Thr Gly Gly Glu Glu  Pro Thr 
        1240                 1245                 1250         


Pro Thr Glu  Ser Pro Thr Glu Thr  Thr Thr Thr Thr Pro  Ser Glu 
        1255                 1260                 1265         


Thr Thr Thr  Thr Thr Ser Thr Thr  Thr Gly Pro Ser Ser  Thr Thr 
        1270                 1275                 1280         


Thr Ser Thr  Pro Gly Gly Gly Ile  Cys Gly Pro Gly Ile  Ile Ala 
        1285                 1290                 1295         


Gly Leu Ala  Leu Ile Pro Leu Leu  Leu Lys Arg Arg Asn  
        1300                 1305                 1310 


<210>  12
<211>  809
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hybrid pullulanase of Thermoccus hydrothermalis and Thermococcus 
       litoralis


<220>
<221>  SIGNAL
<222>  (1)..(27)

<220>
<221>  mat_peptide
<222>  (28)..(809)

<400>  12

Met Lys Lys Pro Leu Gly Lys Ile Val Ala Ser Thr Ala Leu Leu Ile 
        -25                 -20                 -15             


Ser Val Ala Phe Ser Ser Ser Ile Ala Ser Ala Glu Glu Pro Lys Pro 
    -10                 -5              -1  1               5   


Leu Asn Val Ile Ile Val Trp His Gln His Gln Pro Tyr Tyr Tyr Asp 
                10                  15                  20      


Pro Ile Gln Asp Ile Tyr Thr Arg Pro Trp Val Arg Leu His Ala Ala 
            25                  30                  35          


Asn Asn Tyr Trp Lys Met Ala Asn Tyr Leu Ser Lys Tyr Pro Asp Val 
        40                  45                  50              


His Val Ala Ile Asp Leu Ser Gly Ser Leu Ile Ala Gln Leu Ala Asp 
    55                  60                  65                  


Tyr Met Asn Gly Lys Lys Asp Thr Tyr Gln Ile Val Thr Glu Lys Ile 
70                  75                  80                  85  


Ala Asn Gly Glu Pro Leu Thr Leu Glu Asp Lys Trp Phe Met Leu Gln 
                90                  95                  100     


Ala Pro Gly Gly Phe Phe Asp His Thr Ile Pro Trp Asn Gly Glu Pro 
            105                 110                 115         


Val Ala Asp Glu Asn Gly Asn Pro Tyr Arg Glu Gln Trp Asp Arg Tyr 
        120                 125                 130             


Ala Glu Leu Lys Asp Lys Arg Asn Asn Ala Phe Lys Lys Tyr Ala Asn 
    135                 140                 145                 


Leu Pro Leu Asn Glu Gln Lys Val Lys Ile Thr Ala Glu Phe Thr Glu 
150                 155                 160                 165 


Gln Asp Tyr Ile Asp Leu Ala Val Leu Phe Asn Leu Ala Trp Ile Asp 
                170                 175                 180     


Tyr Asn Tyr Ile Ile Asn Thr Pro Glu Leu Lys Ala Leu Tyr Asp Lys 
            185                 190                 195         


Val Asp Val Gly Gly Tyr Thr Lys Glu Asp Val Ala Thr Val Leu Lys 
        200                 205                 210             


His Gln Met Trp Leu Leu Asn His Thr Phe Glu Glu His Glu Lys Ile 
    215                 220                 225                 


Asn Tyr Leu Leu Gly Asn Gly Asn Val Glu Val Thr Val Val Pro Tyr 
230                 235                 240                 245 


Ala His Pro Ile Gly Pro Leu Leu Asn Asp Phe Gly Trp Tyr Glu Asp 
                250                 255                 260     


Phe Asp Ala His Val Lys Lys Ala His Glu Leu Tyr Lys Lys Tyr Leu 
            265                 270                 275         


Gly Asp Asn Arg Val Glu Pro Gln Gly Gly Trp Ala Ala Glu Ser Ala 
        280                 285                 290             


Leu Asn Asp Lys Thr Leu Glu Ile Leu Thr Asn Asn Gly Trp Lys Trp 
    295                 300                 305                 


Val Met Thr Asp Gln Met Val Leu Asp Ile Leu Gly Ile Pro Asn Thr 
310                 315                 320                 325 


Val Glu Asn Tyr Tyr Lys Pro Trp Val Ala Glu Phe Asn Gly Lys Lys 
                330                 335                 340     


Ile Tyr Leu Phe Pro Arg Asn His Asp Leu Ser Asp Arg Val Gly Phe 
            345                 350                 355         


Arg Tyr Ser Gly Met Asn Gln Tyr Gln Ala Val Glu Asp Phe Val Asn 
        360                 365                 370             


Glu Leu Leu Lys Val Gln Lys Glu Asn Tyr Asp Gly Ser Leu Val Tyr 
    375                 380                 385                 


Val Val Thr Leu Asp Gly Glu Asn Pro Trp Glu His Tyr Pro Phe Asp 
390                 395                 400                 405 


Gly Lys Ile Phe Leu Glu Glu Leu Tyr Lys Lys Leu Thr Glu Leu Gln 
                410                 415                 420     


Lys Gln Gly Leu Ile Arg Thr Val Thr Pro Ser Glu Tyr Ile Gln Met 
            425                 430                 435         


Tyr Gly Asp Lys Ala Asn Lys Leu Thr Pro Arg Met Met Glu Arg Leu 
        440                 445                 450             


Asp Leu Thr Gly Asp Asn Val Asn Ala Leu Leu Lys Ala Gln Ser Leu 
    455                 460                 465                 


Gly Glu Leu Tyr Asp Met Thr Gly Val Lys Glu Glu Met Gln Trp Pro 
470                 475                 480                 485 


Glu Ser Ser Trp Ile Asp Gly Thr Leu Ser Thr Trp Ile Gly Glu Pro 
                490                 495                 500     


Gln Glu Asn Tyr Gly Trp Tyr Trp Leu Tyr Met Ala Arg Lys Ala Leu 
            505                 510                 515         


Met Glu Asn Lys Asp Lys Met Ser Gln Ala Asp Trp Glu Lys Ala Tyr 
        520                 525                 530             


Glu Tyr Leu Leu Arg Ala Glu Ala Ser Asp Trp Phe Trp Trp Tyr Gly 
    535                 540                 545                 


Ser Asp Gln Asp Ser Gly Gln Asp Tyr Thr Phe Asp Arg Tyr Leu Lys 
550                 555                 560                 565 


Thr Tyr Leu Tyr Glu Met Tyr Lys Leu Ala Gly Val Glu Pro Pro Ser 
                570                 575                 580     


Tyr Leu Phe Gly Asn Tyr Phe Pro Asp Gly Glu Pro Tyr Thr Thr Arg 
            585                 590                 595         


Gly Leu Val Gly Leu Lys Asp Gly Glu Met Lys Asn Phe Ser Ser Met 
        600                 605                 610             


Ser Pro Leu Ala Lys Gly Val Ser Val Tyr Phe Asp Gly Glu Gly Ile 
    615                 620                 625                 


His Phe Ile Val Lys Gly Asn Leu Asp Arg Phe Glu Val Ser Ile Trp 
630                 635                 640                 645 


Glu Lys Asp Glu Arg Val Gly Asn Thr Phe Thr Arg Leu Gln Glu Lys 
                650                 655                 660     


Pro Asp Glu Leu Ser Tyr Phe Met Phe Pro Phe Ser Arg Asp Ser Val 
            665                 670                 675         


Gly Leu Leu Ile Thr Lys His Val Val Tyr Glu Asn Gly Lys Ala Glu 
        680                 685                 690             


Ile Tyr Gly Ala Thr Asp Tyr Glu Lys Ser Glu Lys Leu Gly Glu Ala 
    695                 700                 705                 


Thr Val Lys Asn Thr Ser Glu Gly Ile Glu Val Val Leu Pro Phe Asp 
710                 715                 720                 725 


Tyr Ile Glu Asn Pro Ser Asp Phe Tyr Phe Ala Val Ser Thr Val Lys 
                730                 735                 740     


Asp Gly Asp Leu Glu Val Ile Ser Thr Pro Val Glu Leu Lys Leu Pro 
            745                 750                 755         


Thr Glu Val Lys Gly Val Val Ile Ala Asp Ile Thr Asp Pro Glu Gly 
        760                 765                 770             


Asp Asp His Gly Pro Gly Asn Tyr Thr 
    775                 780         


<210>  13
<211>  412
<212>  PRT
<213>  Pyrococcus furiosus


<220>
<221>  mat_peptide
<222>  (1)..(412)
<223>  Pyrococcus furiosus protease (Pfu)

<400>  13

Ala Glu Leu Glu Gly Leu Asp Glu Ser Ala Ala Gln Val Met Ala Thr 
1               5                   10                  15      


Tyr Val Trp Asn Leu Gly Tyr Asp Gly Ser Gly Ile Thr Ile Gly Ile 
            20                  25                  30          


Ile Asp Thr Gly Ile Asp Ala Ser His Pro Asp Leu Gln Gly Lys Val 
        35                  40                  45              


Ile Gly Trp Val Asp Phe Val Asn Gly Arg Ser Tyr Pro Tyr Asp Asp 
    50                  55                  60                  


His Gly His Gly Thr His Val Ala Ser Ile Ala Ala Gly Thr Gly Ala 
65                  70                  75                  80  


Ala Ser Asn Gly Lys Tyr Lys Gly Met Ala Pro Gly Ala Lys Leu Ala 
                85                  90                  95      


Gly Ile Lys Val Leu Gly Ala Asp Gly Ser Gly Ser Ile Ser Thr Ile 
            100                 105                 110         


Ile Lys Gly Val Glu Trp Ala Val Asp Asn Lys Asp Lys Tyr Gly Ile 
        115                 120                 125             


Lys Val Ile Asn Leu Ser Leu Gly Ser Ser Gln Ser Ser Asp Gly Thr 
    130                 135                 140                 


Asp Ala Leu Ser Gln Ala Val Asn Ala Ala Trp Asp Ala Gly Leu Val 
145                 150                 155                 160 


Val Val Val Ala Ala Gly Asn Ser Gly Pro Asn Lys Tyr Thr Ile Gly 
                165                 170                 175     


Ser Pro Ala Ala Ala Ser Lys Val Ile Thr Val Gly Ala Val Asp Lys 
            180                 185                 190         


Tyr Asp Val Ile Thr Ser Phe Ser Ser Arg Gly Pro Thr Ala Asp Gly 
        195                 200                 205             


Arg Leu Lys Pro Glu Val Val Ala Pro Gly Asn Trp Ile Ile Ala Ala 
    210                 215                 220                 


Arg Ala Ser Gly Thr Ser Met Gly Gln Pro Ile Asn Asp Tyr Tyr Thr 
225                 230                 235                 240 


Ala Ala Pro Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ile Ala 
                245                 250                 255     


Ala Leu Leu Leu Gln Ala His Pro Ser Trp Thr Pro Asp Lys Val Lys 
            260                 265                 270         


Thr Ala Leu Ile Glu Thr Ala Asp Ile Val Lys Pro Asp Glu Ile Ala 
        275                 280                 285             


Asp Ile Ala Tyr Gly Ala Gly Arg Val Asn Ala Tyr Lys Ala Ile Asn 
    290                 295                 300                 


Tyr Asp Asn Tyr Ala Lys Leu Val Phe Thr Gly Tyr Val Ala Asn Lys 
305                 310                 315                 320 


Gly Ser Gln Thr His Gln Phe Val Ile Ser Gly Ala Ser Phe Val Thr 
                325                 330                 335     


Ala Thr Leu Tyr Trp Asp Asn Ala Asn Ser Asp Leu Asp Leu Tyr Leu 
            340                 345                 350         


Tyr Asp Pro Asn Gly Asn Gln Val Asp Tyr Ser Tyr Thr Ala Tyr Tyr 
        355                 360                 365             


Gly Phe Glu Lys Val Gly Tyr Tyr Asn Pro Thr Asp Gly Thr Trp Thr 
    370                 375                 380                 


Ile Lys Val Val Ser Tyr Ser Gly Ser Ala Asn Tyr Gln Val Asp Val 
385                 390                 395                 400 


Val Ser Asp Gly Ser Leu Ser Gln Pro Gly Ser Ser 
                405                 410         


<210>  14
<211>  595
<212>  PRT
<213>  Penicillium oxalicum


<220>
<221>  mat_peptide
<222>  (1)..(595)
<223>  mature Penicillium oxalicum glucoamylase sequence

<400>  14

Arg Pro Asp Pro Lys Gly Gly Asn Leu Thr Pro Phe Ile His Lys Glu 
1               5                   10                  15      


Gly Glu Arg Ser Leu Gln Gly Ile Leu Asp Asn Leu Gly Gly Arg Gly 
            20                  25                  30          


Lys Lys Thr Pro Gly Thr Ala Ala Gly Leu Phe Ile Ala Ser Pro Asn 
        35                  40                  45              


Thr Glu Asn Pro Asn Tyr Tyr Tyr Thr Trp Thr Arg Asp Ser Ala Leu 
    50                  55                  60                  


Thr Ala Lys Cys Leu Ile Asp Leu Phe Glu Asp Ser Arg Ala Lys Phe 
65                  70                  75                  80  


Pro Ile Asp Arg Lys Tyr Leu Glu Thr Gly Ile Arg Asp Tyr Lys Ser 
                85                  90                  95      


Ser Gln Ala Ile Leu Gln Ser Val Ser Asn Pro Ser Gly Thr Leu Lys 
            100                 105                 110         


Asp Gly Ser Gly Leu Gly Glu Pro Lys Phe Glu Ile Asp Leu Asn Pro 
        115                 120                 125             


Phe Ser Gly Ala Trp Gly Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg 
    130                 135                 140                 


Ala Thr Ala Met Ile Thr Tyr Ala Asn Tyr Leu Ile Ser His Gly Gln 
145                 150                 155                 160 


Lys Ser Asp Val Ser Gln Val Met Trp Pro Ile Ile Ala Asn Asp Leu 
                165                 170                 175     


Ala Tyr Val Gly Gln Tyr Trp Asn Asn Thr Gly Phe Asp Leu Trp Glu 
            180                 185                 190         


Glu Val Asp Gly Ser Ser Phe Phe Thr Ile Ala Val Gln His Arg Ala 
        195                 200                 205             


Leu Val Glu Gly Ser Gln Leu Ala Lys Lys Leu Gly Lys Ser Cys Asp 
    210                 215                 220                 


Ala Cys Asp Ser Gln Pro Pro Gln Ile Leu Cys Phe Leu Gln Ser Phe 
225                 230                 235                 240 


Trp Asn Gly Lys Tyr Ile Thr Ser Asn Ile Asn Thr Gln Ala Ser Arg 
                245                 250                 255     


Ser Gly Ile Asp Leu Asp Ser Val Leu Gly Ser Ile His Thr Phe Asp 
            260                 265                 270         


Pro Glu Ala Ala Cys Asp Asp Ala Thr Phe Gln Pro Cys Ser Ala Arg 
        275                 280                 285             


Ala Leu Ala Asn His Lys Val Tyr Val Asp Ser Phe Arg Ser Ile Tyr 
    290                 295                 300                 


Lys Ile Asn Ala Gly Leu Ala Glu Gly Ser Ala Ala Asn Val Gly Arg 
305                 310                 315                 320 


Tyr Pro Glu Asp Val Tyr Gln Gly Gly Asn Pro Trp Tyr Leu Ala Thr 
                325                 330                 335     


Leu Gly Ala Ser Glu Leu Leu Tyr Asp Ala Leu Tyr Gln Trp Asp Arg 
            340                 345                 350         


Leu Gly Lys Leu Glu Val Ser Glu Thr Ser Leu Ser Phe Phe Lys Asp 
        355                 360                 365             


Phe Asp Ala Thr Val Lys Ile Gly Ser Tyr Ser Arg Asn Ser Lys Thr 
    370                 375                 380                 


Tyr Lys Lys Leu Thr Gln Ser Ile Lys Ser Tyr Ala Asp Gly Phe Ile 
385                 390                 395                 400 


Gln Leu Val Gln Gln Tyr Thr Pro Ser Asn Gly Ser Leu Ala Glu Gln 
                405                 410                 415     


Tyr Asp Arg Asn Thr Ala Ala Pro Leu Ser Ala Asn Asp Leu Thr Trp 
            420                 425                 430         


Ser Phe Ala Ser Phe Leu Thr Ala Thr Gln Arg Arg Asp Ala Val Val 
        435                 440                 445             


Pro Pro Ser Trp Gly Ala Lys Ser Ala Asn Lys Val Pro Thr Thr Cys 
    450                 455                 460                 


Ser Ala Ser Pro Val Val Gly Thr Tyr Lys Ala Pro Thr Ala Thr Phe 
465                 470                 475                 480 


Ser Ser Lys Thr Lys Cys Val Pro Ala Lys Asp Ile Val Pro Ile Thr 
                485                 490                 495     


Phe Tyr Leu Ile Glu Asn Thr Tyr Tyr Gly Glu Asn Val Phe Met Ser 
            500                 505                 510         


Gly Asn Ile Thr Ala Leu Gly Asn Trp Asp Ala Lys Lys Gly Phe Pro 
        515                 520                 525             


Leu Thr Ala Asn Leu Tyr Thr Gln Asp Gln Asn Leu Trp Phe Ala Ser 
    530                 535                 540                 


Val Glu Phe Ile Pro Ala Gly Thr Pro Phe Glu Tyr Lys Tyr Tyr Lys 
545                 550                 555                 560 


Val Glu Pro Asn Gly Asp Ile Thr Trp Glu Lys Gly Pro Asn Arg Val 
                565                 570                 575     


Phe Val Ala Pro Thr Gly Cys Pro Val Gln Pro His Ser Asn Asp Val 
            580                 585                 590         


Trp Gln Phe 
        595 


<210>  15
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sense Primer

<400>  15
atgcgtctca ctctattatc aggtg                                             25


<210>  16
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer F

<400>  16
acacaactgg ggatccacca tgcgtctcac tctattatc                              39


<210>  17
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer R

<400>  17
agatctcgag aagcttaaaa ctgccacacg tcgttgg                                37


<210>  18
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer K79V F 18mer

<400>  18
gcagtctttc caattgac                                                     18


<210>  19
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer K79V R 18mer

<400>  19
aattggaaag actgcccg                                                     18


<210>  20
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer F-NP003940

<400>  20
acacaactgg ggatccacca tgcgtctcac tctattatc                              39


<210>  21
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer F-NP003940

<400>  21
agatctcgag aagcttaaaa ctgccacacg tcgttgg                                37


