                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  POLYPEPTIDES HAVING LYSOZYME ACTIVITY AND POLYNUCLEOTIDES 
       ENCODING SAME

<130>  12166-EP-EPA

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  782
<212>  DNA
<213>  Acremonium alcalophilum


<220>
<221>  CDS
<222>  (1)..(133)

<220>
<221>  sig_peptide
<222>  (1)..(57)

<220>
<221>  CDS
<222>  (215)..(345)

<220>
<221>  CDS
<222>  (516)..(779)

<400>  1
atg gtc tct ttc aag cag ctc gcc ctc ctg gca ctg ggc gcc gtc caa         48
Met Val Ser Phe Lys Gln Leu Ala Leu Leu Ala Leu Gly Ala Val Gln           
1               5                   10                  15                

gta cag gcg cag tgc gtc ggc ccg gct atc aat tcc gcg gct ctt aac         96
Val Gln Ala Gln Cys Val Gly Pro Ala Ile Asn Ser Ala Ala Leu Asn           
            20                  25                  30                    

ctc atc aag gag ttt gag gga tgg agg ccc aac att t gtgcgttccc           143
Leu Ile Lys Glu Phe Glu Gly Trp Arg Pro Asn Ile                           
        35                  40                                            

ttctacgtta catcacccag ttcccttgtt attcagacat tatttctata ttcctggcta      203

acactgtaaa g ac  cgc gac ccc gtc ggc ctc ccc acc gtc gga tac ggc       252
             Tyr Arg Asp Pro Val Gly Leu Pro Thr Val Gly Tyr Gly          
             45                  50                  55                   

cac ctc tgc cgc gac tcg agc tgc tct gac gtc cct tac cca att ccc        300
His Leu Cys Arg Asp Ser Ser Cys Ser Asp Val Pro Tyr Pro Ile Pro           
        60                  65                  70                        

ctg tcc gtt gcc aac ggc gag cgt ctc ctt cgg agc gac cta gcg            345
Leu Ser Val Ala Asn Gly Glu Arg Leu Leu Arg Ser Asp Leu Ala               
    75                  80                  85                            

gtgagtctat cccctttgca cttcataaaa cgtcgccttc tctgttgtca ttctacctgg      405

acagcctccc cctatttctc tcttctatct tttcttcttt cccgttctgc aagcttgacc      465

cctgaccaac catatccacc cagacctacc agaactgcat cacgatgcag acg gcc         521
                                                       Thr Ala            
                                                           90             

tcg tcc gtc gtc ctg aat gcg aac cag tac ggc gcc ctg gtc agc tgg        569
Ser Ser Val Val Leu Asn Ala Asn Gln Tyr Gly Ala Leu Val Ser Trp           
                95                  100                 105               

gcc ttc aac gtc ggc tgc ggc gcc acc agc acg tcg act ctg atc cgc        617
Ala Phe Asn Val Gly Cys Gly Ala Thr Ser Thr Ser Thr Leu Ile Arg           
            110                 115                 120                   

cgc ctc aac gcc gga gag agc ccc aac acc gtc gct gcc cag gag ctg        665
Arg Leu Asn Ala Gly Glu Ser Pro Asn Thr Val Ala Ala Gln Glu Leu           
        125                 130                 135                       

cct cgc tgg aac aag gct ggc ggc cag gtc ctg ccc ggc ctg gtg cgc        713
Pro Arg Trp Asn Lys Ala Gly Gly Gln Val Leu Pro Gly Leu Val Arg           
    140                 145                 150                           

cgc cgt gct gcc gag gta gag ctg cat cgt act tcc acc agt gtc cgt        761
Arg Arg Ala Ala Glu Val Glu Leu His Arg Thr Ser Thr Ser Val Arg           
155                 160                 165                 170           

gct ctg cct gct tgc tct tag                                            782
Ala Leu Pro Ala Cys Ser                                                   
                175                                                       


<210>  2
<211>  176
<212>  PRT
<213>  Acremonium alcalophilum

<400>  2

Met Val Ser Phe Lys Gln Leu Ala Leu Leu Ala Leu Gly Ala Val Gln 
1               5                   10                  15      


Val Gln Ala Gln Cys Val Gly Pro Ala Ile Asn Ser Ala Ala Leu Asn 
            20                  25                  30          


Leu Ile Lys Glu Phe Glu Gly Trp Arg Pro Asn Ile Tyr Arg Asp Pro 
        35                  40                  45              


Val Gly Leu Pro Thr Val Gly Tyr Gly His Leu Cys Arg Asp Ser Ser 
    50                  55                  60                  


Cys Ser Asp Val Pro Tyr Pro Ile Pro Leu Ser Val Ala Asn Gly Glu 
65                  70                  75                  80  


Arg Leu Leu Arg Ser Asp Leu Ala Thr Ala Ser Ser Val Val Leu Asn 
                85                  90                  95      


Ala Asn Gln Tyr Gly Ala Leu Val Ser Trp Ala Phe Asn Val Gly Cys 
            100                 105                 110         


Gly Ala Thr Ser Thr Ser Thr Leu Ile Arg Arg Leu Asn Ala Gly Glu 
        115                 120                 125             


Ser Pro Asn Thr Val Ala Ala Gln Glu Leu Pro Arg Trp Asn Lys Ala 
    130                 135                 140                 


Gly Gly Gln Val Leu Pro Gly Leu Val Arg Arg Arg Ala Ala Glu Val 
145                 150                 155                 160 


Glu Leu His Arg Thr Ser Thr Ser Val Arg Ala Leu Pro Ala Cys Ser 
                165                 170                 175     


<210>  3
<211>  838
<212>  DNA
<213>  Acremonium alcalophilum


<220>
<221>  CDS
<222>  (1)..(147)

<220>
<221>  sig_peptide
<222>  (1)..(57)

<220>
<221>  CDS
<222>  (302)..(835)

<400>  3
atg aag ctt ctt ccc tcc ttg att ggc ctg gcc agt ctg gcg tcc ctc         48
Met Lys Leu Leu Pro Ser Leu Ile Gly Leu Ala Ser Leu Ala Ser Leu           
1               5                   10                  15                

gcc gtc gcc cgg atc ccc ggc ttt gac att tcg ggc tgg caa ccg acc         96
Ala Val Ala Arg Ile Pro Gly Phe Asp Ile Ser Gly Trp Gln Pro Thr           
            20                  25                  30                    

acc gac ttt gca agg gcg tat gct aat gga gat cgt ttc gtc tac atc        144
Thr Asp Phe Ala Arg Ala Tyr Ala Asn Gly Asp Arg Phe Val Tyr Ile           
        35                  40                  45                        

aag gtacgttcaa ccttgccacc aagttgcgaa cccgagacaa gactgtgacc             197
Lys                                                                       
                                                                          

gcctcctttg ccctggggca gctcacgcac ccagcagcat cccatccccc ggccccccac      257

gtaccaccgg aaagctaaca tcaaccccct accactgcta ccag gcc acc gag ggc       313
                                                 Ala Thr Glu Gly          
                                                 50                       

acc aca ttc aag agc tcc gca ttc agc cgc cag tac acc ggc gca acg        361
Thr Thr Phe Lys Ser Ser Ala Phe Ser Arg Gln Tyr Thr Gly Ala Thr           
    55                  60                  65                            

caa aac ggc ttc atc cgc ggc gcc tac cac ttc gcc cag ccc gcc gcg        409
Gln Asn Gly Phe Ile Arg Gly Ala Tyr His Phe Ala Gln Pro Ala Ala           
70                  75                  80                  85            

tcc tcg ggc gcc gcg cag gcg aga tac ttc gcc agc aac ggc ggc ggc        457
Ser Ser Gly Ala Ala Gln Ala Arg Tyr Phe Ala Ser Asn Gly Gly Gly           
                90                  95                  100               

tgg tcc aag gac ggc atc acc ctg ccc ggg gcg ctg gac atc gag tac        505
Trp Ser Lys Asp Gly Ile Thr Leu Pro Gly Ala Leu Asp Ile Glu Tyr           
            105                 110                 115                   

aac ccc aac ggc gcc acc tgc tac ggc ctc tcg caa tcg gcc atg gtg        553
Asn Pro Asn Gly Ala Thr Cys Tyr Gly Leu Ser Gln Ser Ala Met Val           
        120                 125                 130                       

aac tgg atc gag gac ttt gtc acc acc tac cac ggc atc acc tcc cgc        601
Asn Trp Ile Glu Asp Phe Val Thr Thr Tyr His Gly Ile Thr Ser Arg           
    135                 140                 145                           

tgg ccc gtc atc tac acc acc acc gac tgg tgg acc cag tgc acc ggc        649
Trp Pro Val Ile Tyr Thr Thr Thr Asp Trp Trp Thr Gln Cys Thr Gly           
150                 155                 160                 165           

aac tcc aac cgc ttc gcg aac cgc tgc ccg ctg tgg atc gcc cgc tac        697
Asn Ser Asn Arg Phe Ala Asn Arg Cys Pro Leu Trp Ile Ala Arg Tyr           
                170                 175                 180               

gcc agc tcc gtc ggc act ctg ccc aat ggc tgg ggc ttt tac acc ttc        745
Ala Ser Ser Val Gly Thr Leu Pro Asn Gly Trp Gly Phe Tyr Thr Phe           
            185                 190                 195                   

tgg cag tac aac gac aag tat cct cag ggc ggt gat tcg aac tgg ttc        793
Trp Gln Tyr Asn Asp Lys Tyr Pro Gln Gly Gly Asp Ser Asn Trp Phe           
        200                 205                 210                       

aac ggc gat gcg tcg cgt ctc agg gct ctc gct aac gga gac taa            838
Asn Gly Asp Ala Ser Arg Leu Arg Ala Leu Ala Asn Gly Asp                   
    215                 220                 225                           


<210>  4
<211>  227
<212>  PRT
<213>  Acremonium alcalophilum

<400>  4

Met Lys Leu Leu Pro Ser Leu Ile Gly Leu Ala Ser Leu Ala Ser Leu 
1               5                   10                  15      


Ala Val Ala Arg Ile Pro Gly Phe Asp Ile Ser Gly Trp Gln Pro Thr 
            20                  25                  30          


Thr Asp Phe Ala Arg Ala Tyr Ala Asn Gly Asp Arg Phe Val Tyr Ile 
        35                  40                  45              


Lys Ala Thr Glu Gly Thr Thr Phe Lys Ser Ser Ala Phe Ser Arg Gln 
    50                  55                  60                  


Tyr Thr Gly Ala Thr Gln Asn Gly Phe Ile Arg Gly Ala Tyr His Phe 
65                  70                  75                  80  


Ala Gln Pro Ala Ala Ser Ser Gly Ala Ala Gln Ala Arg Tyr Phe Ala 
                85                  90                  95      


Ser Asn Gly Gly Gly Trp Ser Lys Asp Gly Ile Thr Leu Pro Gly Ala 
            100                 105                 110         


Leu Asp Ile Glu Tyr Asn Pro Asn Gly Ala Thr Cys Tyr Gly Leu Ser 
        115                 120                 125             


Gln Ser Ala Met Val Asn Trp Ile Glu Asp Phe Val Thr Thr Tyr His 
    130                 135                 140                 


Gly Ile Thr Ser Arg Trp Pro Val Ile Tyr Thr Thr Thr Asp Trp Trp 
145                 150                 155                 160 


Thr Gln Cys Thr Gly Asn Ser Asn Arg Phe Ala Asn Arg Cys Pro Leu 
                165                 170                 175     


Trp Ile Ala Arg Tyr Ala Ser Ser Val Gly Thr Leu Pro Asn Gly Trp 
            180                 185                 190         


Gly Phe Tyr Thr Phe Trp Gln Tyr Asn Asp Lys Tyr Pro Gln Gly Gly 
        195                 200                 205             


Asp Ser Asn Trp Phe Asn Gly Asp Ala Ser Arg Leu Arg Ala Leu Ala 
    210                 215                 220                 


Asn Gly Asp 
225         


<210>  5
<211>  41
<212>  DNA
<213>  F-P242M9

<400>  5
acacaactgg ggatccacca tgaagcttct tccctccttg a                           41


<210>  6
<211>  38
<212>  DNA
<213>  R-P242M9

<400>  6
agatctcgag aagcttatta gtctccgtta gcgagagc                               38


<210>  7
<211>  747
<212>  DNA
<213>  artificial

<220>
<223>  Construct


<220>
<221>  CDS
<222>  (1)..(744)

<220>
<221>  sig_peptide
<222>  (1)..(69)

<220>
<221>  mat_peptide
<222>  (121)..(744)

<400>  7
atg aag ttc ttc acc acc atc ctc agc acc gcc agc ctt gtt gct gct         48
Met Lys Phe Phe Thr Thr Ile Leu Ser Thr Ala Ser Leu Val Ala Ala           
-40                 -35                 -30                 -25           

ctc ccc gcc gct gtt gac tcg aac cat acc ccg gcc gct cct gaa ctt         96
Leu Pro Ala Ala Val Asp Ser Asn His Thr Pro Ala Ala Pro Glu Leu           
                -20                 -15                 -10               

gtt gcc cgg agt cct att cgt cga cgc att ccc gga ttc gat atc tcg        144
Val Ala Arg Ser Pro Ile Arg Arg Arg Ile Pro Gly Phe Asp Ile Ser           
            -5              -1  1               5                         

gga tgg cag ccg acg acg gac ttc gca agg gcg tac gca aac gga gac        192
Gly Trp Gln Pro Thr Thr Asp Phe Ala Arg Ala Tyr Ala Asn Gly Asp           
    10                  15                  20                            

cga ttc gtg tac atc aag gca aca gag gga aca aca ttc aaa tcg tcg        240
Arg Phe Val Tyr Ile Lys Ala Thr Glu Gly Thr Thr Phe Lys Ser Ser           
25                  30                  35                  40            

gca ttc tcc agg cag tac acc gga gca acc cag aac ggc ttc atc cga        288
Ala Phe Ser Arg Gln Tyr Thr Gly Ala Thr Gln Asn Gly Phe Ile Arg           
                45                  50                  55                

gga gcc tac cac ttc gcc cag cct gca gcc tcc tcg gga gca gcc cag        336
Gly Ala Tyr His Phe Ala Gln Pro Ala Ala Ser Ser Gly Ala Ala Gln           
            60                  65                  70                    

gca agg tac ttc gca tcg aac ggt ggc ggt tgg tcc aag gac ggt atc        384
Ala Arg Tyr Phe Ala Ser Asn Gly Gly Gly Trp Ser Lys Asp Gly Ile           
        75                  80                  85                        

acc ctc cct ggt gcc ttg gat atc gag tac aac ccc aac gga gca aca        432
Thr Leu Pro Gly Ala Leu Asp Ile Glu Tyr Asn Pro Asn Gly Ala Thr           
    90                  95                  100                           

tgt tat ggt ctc tcg cag tcg gcg atg gtg aac tgg att gag gac ttc        480
Cys Tyr Gly Leu Ser Gln Ser Ala Met Val Asn Trp Ile Glu Asp Phe           
105                 110                 115                 120           

gtg aca acc tac cac ggc atc acc tcg agg tgg cct gtg atc tac acc        528
Val Thr Thr Tyr His Gly Ile Thr Ser Arg Trp Pro Val Ile Tyr Thr           
                125                 130                 135               

aca acc gac tgg tgg acg cag tgt acc ggc aac tcc aac cga ttc gcg        576
Thr Thr Asp Trp Trp Thr Gln Cys Thr Gly Asn Ser Asn Arg Phe Ala           
            140                 145                 150                   

aac agg tgt ccg ctc tgg atc gcg agg tat gcc tcc tcc gtc ggc acc        624
Asn Arg Cys Pro Leu Trp Ile Ala Arg Tyr Ala Ser Ser Val Gly Thr           
        155                 160                 165                       

ctc ccg aac gga tgg ggc ttc tat acc ttc tgg cag tac aac gat aag        672
Leu Pro Asn Gly Trp Gly Phe Tyr Thr Phe Trp Gln Tyr Asn Asp Lys           
    170                 175                 180                           

tac ccc cag gga gga gat tcc aac tgg ttc aac ggt gat gca tcg agg        720
Tyr Pro Gln Gly Gly Asp Ser Asn Trp Phe Asn Gly Asp Ala Ser Arg           
185                 190                 195                 200           

ctc agg gca ttg gcg aac ggc gat tag                                    747
Leu Arg Ala Leu Ala Asn Gly Asp                                           
                205                                                       


<210>  8
<211>  248
<212>  PRT
<213>  artificial

<220>
<223>  Synthetic Construct

<400>  8

Met Lys Phe Phe Thr Thr Ile Leu Ser Thr Ala Ser Leu Val Ala Ala 
-40                 -35                 -30                 -25 


Leu Pro Ala Ala Val Asp Ser Asn His Thr Pro Ala Ala Pro Glu Leu 
                -20                 -15                 -10     


Val Ala Arg Ser Pro Ile Arg Arg Arg Ile Pro Gly Phe Asp Ile Ser 
            -5              -1  1               5               


Gly Trp Gln Pro Thr Thr Asp Phe Ala Arg Ala Tyr Ala Asn Gly Asp 
    10                  15                  20                  


Arg Phe Val Tyr Ile Lys Ala Thr Glu Gly Thr Thr Phe Lys Ser Ser 
25                  30                  35                  40  


Ala Phe Ser Arg Gln Tyr Thr Gly Ala Thr Gln Asn Gly Phe Ile Arg 
                45                  50                  55      


Gly Ala Tyr His Phe Ala Gln Pro Ala Ala Ser Ser Gly Ala Ala Gln 
            60                  65                  70          


Ala Arg Tyr Phe Ala Ser Asn Gly Gly Gly Trp Ser Lys Asp Gly Ile 
        75                  80                  85              


Thr Leu Pro Gly Ala Leu Asp Ile Glu Tyr Asn Pro Asn Gly Ala Thr 
    90                  95                  100                 


Cys Tyr Gly Leu Ser Gln Ser Ala Met Val Asn Trp Ile Glu Asp Phe 
105                 110                 115                 120 


Val Thr Thr Tyr His Gly Ile Thr Ser Arg Trp Pro Val Ile Tyr Thr 
                125                 130                 135     


Thr Thr Asp Trp Trp Thr Gln Cys Thr Gly Asn Ser Asn Arg Phe Ala 
            140                 145                 150         


Asn Arg Cys Pro Leu Trp Ile Ala Arg Tyr Ala Ser Ser Val Gly Thr 
        155                 160                 165             


Leu Pro Asn Gly Trp Gly Phe Tyr Thr Phe Trp Gln Tyr Asn Asp Lys 
    170                 175                 180                 


Tyr Pro Gln Gly Gly Asp Ser Asn Trp Phe Asn Gly Asp Ala Ser Arg 
185                 190                 195                 200 


Leu Arg Ala Leu Ala Asn Gly Asp 
                205             


<210>  9
<211>  46
<212>  DNA
<213>  BamHI

<400>  9
tacacaactg gggatccagc ggccgcacca tgaagttctt caccac                      46


<210>  10
<211>  37
<212>  DNA
<213>  EcoRI

<400>  10
atacttgtcc gaattcctaa tcgccgttcg ccaatgc                                37


