                         SEQUENCE LISTING

<110>  ALERE SAN DIEGO, INC.
       EHRICHT, Ralf
       MONECKE, Stefan
       REJMAN, John J.
       BUECHLER, Joseph
 
<120>  DEVICES AND METHODS FOR DETECTION OF PANTON-VALENTINE LEUKOCIDIN 
       (PVL)

<130>  ALERE1450-4WO

<150>  US 61/714,649
<151>  2012-10-16

<150>  US 61/617,974
<151>  2012-03-30

<150>  US 61/561,767
<151>  2011-11-18

<150>  US 61/558,848
<151>  2011-11-11

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  660
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  1
gacgttgtga tgtcacagtc tccatcctcc ctgactgtga cagcaggaga gaaggtcact       60

atgagctgca agtccagtca gagtctgtta ctcagtggaa atcaaaagaa cctcttgacc      120

tggttccagc agaaaccagg gcagcctcct aaactgttga tctactgggc atccactagg      180

gaatctgggg tccctgatcg cttcacaggc agtggatctg gaacagattt cactctcacc      240

atcagcagtg tgcaggctga agacctggca gtttattact gtcagaatga ttataattat      300

ccgtacacgt tcggaggggg gaccaagctg gaaataaaac gggctgatgc tgcaccaact      360

gtatccatct tcccaccatc cagtgagcag ttaacatctg gaggtgcctc agtcgtgtgc      420

ttcttgaaca acttctaccc caaagacatc aatgtcaagt ggaagattga tggcagtgaa      480

cgacaaaatg gcgtcctgaa cagttggact gatcaggaca gcaaagacag cacctacagc      540

atgagcagca ccctcacgtt gaccaaggac gagtatgaac gacataacag ctatacctgt      600

gaggccactc acaagacatc aacttcaccc attgtcaaga gcttcaacag gaatgagtct      660


<210>  2
<211>  220
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  2

Asp Val Val Met Ser Gln Ser Pro Ser Ser Leu Thr Val Thr Ala Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser Leu Leu Leu Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Leu Leu Thr Trp Phe Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp Tyr Asn Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser 
        115                 120                 125             


Glu Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn 
    130                 135                 140                 


Phe Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu 
145                 150                 155                 160 


Arg Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp 
                165                 170                 175     


Ser Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr 
            180                 185                 190         


Glu Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr 
        195                 200                 205             


Ser Pro Ile Val Lys Ser Phe Asn Arg Asn Glu Ser 
    210                 215                 220 


<210>  3
<211>  681
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  3
caggtccagc tgcagcagtc tgggcctcag ctggttaggc ctggggcttc agtgaagata       60

tcctgcaagg cttctggtca ctcattcacc acctactgga tgcactgggt gaagcagagg      120

cctggacaag gtcttgagtg gattggcatg attgatcctt ccgatagtga aactaggtta      180

aatcagaagt tcaaggacaa ggccacattg actgtagaca aatcctccag cacagtctac      240

atgcaactca gcagcccgac atctgaagac tctgtggtct attactgtgc aagctactat      300

ggcaattcta tggactactg gggtcaagga acctcagtca ccgtctcctc agccaaaacg      360

acacccccat ctgtctatcc actggcccct ggatctgctg cccaaactaa ctccatggtg      420

accctgggat gcctggtcaa gggctatttc cctgagccag tgacagtgac ctggaactct      480

ggatccctgt ccagcggtgt gcacaccttc ccagctgtcc tgcagtctga cctctacact      540

ctgagcagct cagtgactgt cccctccagc acctggccca gcgagaccgt cacctgcaac      600

gttgcccacc cggccagcag caccaaggtg gacaagaaaa ttgtgcccag ggattgtcat      660

catcaccatc accatcacta a                                                681


<210>  4
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  4

Gln Val Gln Leu Gln Gln Ser Gly Pro Gln Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly His Ser Phe Thr Thr Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Met Ile Asp Pro Ser Asp Ser Glu Thr Arg Leu Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Val Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Pro Thr Ser Glu Asp Ser Val Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ser Tyr Tyr Gly Asn Ser Met Asp Tyr Trp Gly Gln Gly Thr Ser 
            100                 105                 110         


Val Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu 
        115                 120                 125             


Ala Pro Gly Ser Ala Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser 
145                 150                 155                 160 


Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Pro Ser Ser Thr Trp 
            180                 185                 190         


Pro Ser Glu Thr Val Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr 
        195                 200                 205             


Lys Val Asp Lys Lys Ile Val Pro Arg Asp Cys His His His His His 
    210                 215                 220                 


His His 
225     


<210>  5
<211>  657
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  5
gatgttgtgg tgactcaaac tccactctcc ctgcctgtca gtcttggaga tcaagcctcc       60

atctcttgca gatctagtca gagccttgta cacagtgatg gaaacaccta tttacattgg      120

tacctgcaga agccaggcca gtctccaaag ctcctgatct acaaagtttc caaccgattt      180

tctggggtcc cagacaggtt cagtggcagt ggatcaggga cagatttcac actcaagatc      240

agcagagtgg aggctgaaga tctgggagtt tatttctgct ctcaaaatac acatgttcca      300

ttcacattcg gctcggggac aaagttggaa ataaaacggg ctgatgctgc accaactgta      360

tccatcttcc caccatccag tgagcagtta acatctggag gtgcctcagt cgtgtgcttc      420

ttgaacaact tctaccccaa agacatcaat gtcaagtgga agattgatgg cagtgaacga      480

caaaatggcg tcctgaacag ttggactgat caggacagca aagacagcac ctacagcatg      540

agcagcaccc tcacgttgac caaggacgag tatgaacgac ataacagcta tacctgtgag      600

gccactcaca agacatcaac ttcacccatt gtcaagagct tcaacaggaa tgagtct         657


<210>  6
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  6

Asp Val Val Val Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asp Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Asn 
                85                  90                  95      


Thr His Val Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu 
        115                 120                 125             


Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg 
145                 150                 155                 160 


Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu 
            180                 185                 190         


Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser 
        195                 200                 205             


Pro Ile Val Lys Ser Phe Asn Arg Asn Glu Ser 
    210                 215                 


<210>  7
<211>  681
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  7
gagatccagc tgcagcagtc tggagctgag ctggggaggc ctgggtcctc agtgaagctg       60

tcctgcaaga cttctggata tactttcaca aacttttata taacctggct gaaacagagg      120

cctggacagg gcctggaatg gattggattt atttatcctg gaaatggtta tactgcatac      180

aatgagaaat tccagggaga ggccacactg acttcagaca catcttccag cacagcctac      240

atgcacctca gaagcctgac atctgaggac tctgcaatct atttctgtgc aagactggga      300

cgaaatgaag ttgactactg gggccaaggc accactctca cagtctcctc agccaaaacg      360

acacccccat ctgtctatcc actggcccct ggatctgctg cccaaactaa ctccatggtg      420

accctgggat gcctggtcaa gggctatttc cctgagccag tgacagtgac ctggaactct      480

ggatccctgt ccagcggtgt gcacaccttc ccagctgtcc tgcagtctga cctctacact      540

ctgagcagct cagtgactgt cccctccagc acctggccca gcgagaccgt cacctgcaac      600

gttgcccacc cggccagcag caccaaggtg gacaagaaaa ttgtgcccag ggattgtcat      660

catcaccatc accatcacta a                                                681


<210>  8
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  8

Glu Ile Gln Leu Gln Gln Ser Gly Ala Glu Leu Gly Arg Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Asn Phe 
            20                  25                  30          


Tyr Ile Thr Trp Leu Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Phe Ile Tyr Pro Gly Asn Gly Tyr Thr Ala Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Gln Gly Glu Ala Thr Leu Thr Ser Asp Thr Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met His Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Ile Tyr Phe Cys 
                85                  90                  95      


Ala Arg Leu Gly Arg Asn Glu Val Asp Tyr Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu 
        115                 120                 125             


Ala Pro Gly Ser Ala Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser 
145                 150                 155                 160 


Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Pro Ser Ser Thr Trp 
            180                 185                 190         


Pro Ser Glu Thr Val Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr 
        195                 200                 205             


Lys Val Asp Lys Lys Ile Val Pro Arg Asp Cys His His His His His 
    210                 215                 220                 


His His 
225     


<210>  9
<211>  660
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  9
gacatcgtta tgtctcagtc tccatcctcc ctagctgtgt cagttggaga gacggttact       60

atgagctgca agtccagtca gaccctttta tatagtagca atcaaaagaa ttacttggcc      120

tggtaccagc agaaaccagg acagtctcct aaattgctga tttactgggc atccactagg      180

gaatctgggg tcccagatcg cttcacaggc agtggatctg ggacagattt cactctcacc      240

atcagcagtg tgaaggctga agacctggca gtttattact gtcagcaata ttatgactat      300

ccgctcacgt tcggtgctgg gaccaagctg gagctgaaac gggctgatgc tgcaccaact      360

gtatccatct tcccaccatc cagtgagcag ttaacatctg gaggtgcctc agtcgtgtgc      420

ttcttgaaca acttctaccc caaagacatc aatgtcaagt ggaagattga tggcagtgaa      480

cgacaaaatg gcgtcctgaa cagttggact gatcaggaca gcaaagacag cacctacagc      540

atgagcagca ccctcacgtt gaccaaggac gagtatgaac gacataacag ctatacctgt      600

gaggccactc acaagacatc aacttcaccc attgtcaaga gcttcaacag gaatgagtct      660


<210>  10
<211>  220
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  10

Asp Ile Val Met Ser Gln Ser Pro Ser Ser Leu Ala Val Ser Val Gly 
1               5                   10                  15      


Glu Thr Val Thr Met Ser Cys Lys Ser Ser Gln Thr Leu Leu Tyr Ser 
            20                  25                  30          


Ser Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Lys Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Asp Tyr Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 
            100                 105                 110         


Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser 
        115                 120                 125             


Glu Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn 
    130                 135                 140                 


Phe Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu 
145                 150                 155                 160 


Arg Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp 
                165                 170                 175     


Ser Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr 
            180                 185                 190         


Glu Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr 
        195                 200                 205             


Ser Pro Ile Val Lys Ser Phe Asn Arg Asn Glu Ser 
    210                 215                 220 


<210>  11
<211>  684
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  11
gacgtgcagg tggtggagtc tgggggaggc ttagtgaagc ctggagggtc cctgaaactc       60

tcctgtgcag tctctggatt cactttcagt agttatcaca tgtcttgggt tcgccagact      120

ccggcgaaga ggctggagtg ggtcgcaacc attagtggtg gtggtggtaa cacctactat      180

ccagacagtg tgaagggccg attcaccatc tccagagaca atgccaagaa caccctgttc      240

ctgcaaatga gcagtctgag gtctgaggac acggccttgt attactgtgc aagacatgag      300

ggtccttact actccttcga tgtctggggc acagggacca cggtcaccgt ctcttcagcc      360

aaaacgacac ccccatctgt ctatccactg gcccctggat ctgctgccca aactaactcc      420

atggtgaccc tgggatgcct ggtcaagggc tatttccctg agccagtgac agtgacctgg      480

aactctggat ccctgtccag cggtgtgcac accttcccag ctgtcctgca gtctgacctc      540

tacactctga gcagctcagt gactgtcccc tccagcacct ggcccagcga gaccgtcacc      600

tgcaacgttg cccacccggc cagcagcacc aaggtggaca agaaaattgt gcccagggat      660

tgtcatcatc accatcacca tcac                                             684


<210>  12
<211>  228
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  12

Asp Val Gln Val Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Val Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


His Met Ser Trp Val Arg Gln Thr Pro Ala Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Ser Gly Gly Gly Gly Asn Thr Tyr Tyr Pro Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Phe 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Ala Arg His Glu Gly Pro Tyr Tyr Ser Phe Asp Val Trp Gly Thr Gly 
            100                 105                 110         


Thr Thr Val Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr 
        115                 120                 125             


Pro Leu Ala Pro Gly Ser Ala Ala Gln Thr Asn Ser Met Val Thr Leu 
    130                 135                 140                 


Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp 
145                 150                 155                 160 


Asn Ser Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     


Gln Ser Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Pro Ser Ser 
            180                 185                 190         


Thr Trp Pro Ser Glu Thr Val Thr Cys Asn Val Ala His Pro Ala Ser 
        195                 200                 205             


Ser Thr Lys Val Asp Lys Lys Ile Val Pro Arg Asp Cys His His His 
    210                 215                 220                 


His His His His 
225             


<210>  13
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  13
cctgaattca tgaaaaaaat agtcaaatc                                         29


<210>  14
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  14
atagcggccg cttagctcat aggatttt                                          28


