                         SEQUENCE LISTING

<110>  Richter Gedeon Nyrt.
 
<120>  Method for the production of polypeptides

<130>  HE 162 843

<150>  HU P1200171
<151>  2012-03-19

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  174
<212>  PRT
<213>  Homo sapiens

<400>  1

Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys 
1               5                   10                  15      


Cys Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln 
            20                  25                  30          


Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val 
        35                  40                  45              


Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys 
    50                  55                  60                  


Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser 
65                  70                  75                  80  


Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser 
                85                  90                  95      


Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp 
            100                 105                 110         


Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro 
        115                 120                 125             


Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe 
    130                 135                 140                 


Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe 
145                 150                 155                 160 


Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro 
                165                 170                 


<210>  2
<211>  174
<212>  PRT
<213>  Bos taurus

<400>  2

Thr Pro Leu Gly Pro Ala Arg Ser Leu Pro Gln Ser Phe Leu Leu Lys 
1               5                   10                  15      


Cys Leu Glu Gln Val Arg Lys Ile Gln Ala Asp Gly Ala Glu Leu Gln 
            20                  25                  30          


Glu Arg Leu Cys Ala Ala His Lys Leu Cys His Pro Glu Glu Leu Met 
        35                  40                  45              


Leu Leu Arg His Ser Leu Gly Ile Pro Gln Ala Pro Leu Ser Ser Cys 
    50                  55                  60                  


Ser Ser Gln Ser Leu Gln Leu Arg Gly Cys Leu Asn Gln Leu His Gly 
65                  70                  75                  80  


Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Ala Gly Ile Ser 
                85                  90                  95      


Pro Glu Leu Ala Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Thr Asp 
            100                 105                 110         


Phe Ala Thr Asn Ile Trp Leu Gln Met Glu Asp Leu Gly Ala Ala Pro 
        115                 120                 125             


Ala Val Gln Pro Thr Gln Gly Ala Met Pro Thr Phe Thr Ser Ala Phe 
    130                 135                 140                 


Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser Gln Leu His Arg Phe 
145                 150                 155                 160 


Leu Glu Leu Ala Tyr Arg Gly Leu Arg Tyr Leu Ala Glu Pro 
                165                 170                 


<210>  3
<211>  175
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu 
1               5                   10                  15      


Lys Cys Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu 
            20                  25                  30          


Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu 
        35                  40                  45              


Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser 
    50                  55                  60                  


Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His 
65                  70                  75                  80  


Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile 
                85                  90                  95      


Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala 
            100                 105                 110         


Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala 
        115                 120                 125             


Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala 
    130                 135                 140                 


Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser 
145                 150                 155                 160 


Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro 
                165                 170                 175 


<210>  4
<211>  5234
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the G-CSF vector

<400>  4
caattcttag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt       60

atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca      120

gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat      180

acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt      240

gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac      300

aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg      360

tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg      420

aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc      480

aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca      540

tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag      600

ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt      660

cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg      720

cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa      780

tcgcggccta gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact      840

gtttatgtaa gcagacagtt ttattgttca tgaccaaaat cccttaacgt gagttttcgt      900

tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc      960

tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc     1020

cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac     1080

caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac     1140

cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt     1200

cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct     1260

gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat     1320

acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt     1380

atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg     1440

cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt     1500

gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt     1560

tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg     1620

tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg     1680

agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta     1740

cgcatctgtg cggtatttca caccgcaatg gtgcactctc agtacaatct gctctgatgc     1800

cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc     1860

cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct     1920

tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca     1980

ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag     2040

atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg     2100

cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc     2160

gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc     2220

acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa     2280

ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc     2340

gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg     2400

aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg     2460

aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt     2520

cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg     2580

gtcctcaacg acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg     2640

gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg     2700

tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg     2760

tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag     2820

aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact     2880

gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt     2940

acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg     3000

cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt     3060

ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga     3120

gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt     3180

ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat     3240

gtccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg     3300

atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg     3360

ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt     3420

gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg     3480

gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg     3540

cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag     3600

aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag     3660

cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt     3720

acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc     3780

ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt     3840

ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat     3900

gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct     3960

ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc     4020

gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca     4080

tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct     4140

tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg     4200

ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg     4260

ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc     4320

catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg     4380

ccacgatgcg tccggcgtag aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac     4440

tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact     4500

ttaagaagga gatatacata tgactccatt aggtcctgct tcttctctgc cgcaaagctt     4560

tctgctgaaa tgtctggaac aggttcgtaa aatccagggt gacggtgctg cactgcaaga     4620

aaaactgtgc gctacttaca aactgtgcca tccggaagag ctggtactgc tgggtcattc     4680

tcttgggatc ccgtgggctc cgctgtcttc ttgtccatct caagctcttc agctggctgg     4740

ttgtctgtct caactgcatt ctggtctgtt cctgtatcag ggtcttctgc aagctctgga     4800

aggtatctct ccggaactgg gtccgactct ggacactctg cagctagatg tagctgactt     4860

tgctactact atttggcaac agatggaaga gctcggtatg gcaccagctc tgcaaccgac     4920

tcaaggtgct atgccggcat tcgcttctgc attccagcgt cgtgcaggag gtgtactggt     4980

tgcttctcat ctgcaatctt tcctggaagt atcttaccgt gttctgcgtc atctggctca     5040

gccgtaataa gctcgagcac caccaccacc accaccacca ctaattgatt aatacctagg     5100

ctgctaaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta     5160

gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact     5220

atatccggat ctag                                                       5234


<210>  5
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence IntLambd 1

<400>  5
gtccgactta tgcccgagaa gatgttgagc aaacttatcg cttatc                      46


<210>  6
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence IntLambd 2

<400>  6
tgcaaagaga ttcttggcgg agaaaccata attgcatcta ctcg                        44


