SEQUENCE LISTING

<110>  Pieris AG
 
<120>  Novel uses of VEGF antagonists

<130>  PIE14344PCT

<150>  US 61/558,730
<151>  2011-11-11

<160>  16

<170>  PatentIn version 3.5


<210>  1
<211>  158
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of human tear lipocalin with binding affinity for VEGF

<400>  1

Leu Leu His His Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr 
1               5                   10                  15      


Trp Tyr Leu Lys Ala Met Thr Val Asp Val Gly Ala Leu Arg Cys Leu 
            20                  25                  30          


Ala Gly Ser Val Ile Pro Thr Thr Leu Thr Thr Leu Glu Gly Gly Asn
        35                  40                  45              


Leu Glu Ala Lys Val Thr Met His Ile Lys Gly Arg Ser Gln Glu Val
    50                  55                  60                  


Lys Ala Val Leu Ser Lys Thr Asp Glu Pro Gly Ile Tyr Thr Ala Ile 
65                  70                  75                  80  


Gly Gly Ile His Val Ala Lys Ile Gly Arg Ser His Val Lys Cys His 
                85                  90                  95      


Tyr Ile Phe Tyr Ser Glu Gly Cys Leu Ser Gly Val Pro Val Pro Gly 
            100                 105                 110         


Val Trp Leu Val Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu
        115                 120                 125             


Asp Phe Glu Lys Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile
    130                 135                 140                 


Leu Ile Pro Arg Gln Ser Glu Thr Ser Ser Pro Gly Ser Ala 
145                 150                 155              


<210>  2
<211>  158
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of human tear lipocalin with binding affinity for VEGF

<400>  2

Leu Leu His His Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr 
1               5                   10                  15      


Trp Tyr Leu Lys Ala Met Thr Val Asp Val Gly Ala Leu Arg Cys Leu 
            20                  25                  30          


Ala Gly Ser Val Ile Pro Thr Thr Leu Thr Thr Leu Glu Gly Gly Asn
        35                  40                  45              


Leu Glu Ala Lys Val Thr Met His Ile Lys Gly Arg Ser Gln Glu Val
    50                  55                  60                  


Lys Ala Val Leu Ser Lys Thr Asp Glu Pro Gly Ile Tyr Thr Ala Ile 
65                  70                  75                  80  


Gly Gly Ile His Val Ala Lys Ile Gly Arg Ser His Val Lys Asp His 
                85                  90                  95      


Tyr Ile Phe Tyr Ser Glu Gly Cys Leu Ser Gly Val Pro Val Pro Gly 
            100                 105                 110         


Val Trp Leu Val Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu
        115                 120                 125             


Asp Phe Glu Lys Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile
    130                 135                 140                 


Leu Ile Pro Arg Gln Ser Glu Thr Ser Ser Pro Gly Ser Ala 
145                 150                 155              


<210>  3
<211>  158
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of human tear lipocalin with binding affinity for VEGF

<400>  3

Leu Leu His His Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr 
1               5                   10                  15      


Trp Tyr Leu Lys Ala Met Thr Val Asp Ser Gly Ala Leu Arg Cys Leu
            20                  25                  30          


Ala Gly Ser Val Ile Pro Thr Thr Leu Thr Thr Leu Glu Gly Gly Asn
        35                  40                  45              


Leu Glu Ala Lys Val Thr Met His Ile Lys Gly Arg Ser Gln Glu Val
    50                  55                  60                  


Lys Ala Val Leu Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Ile 
65                  70                  75                  80  


Gly Gly Ile His Val Ala His Ile Ile Arg Ser His Val Lys Asp His
                85                  90                  95      


Tyr Ile Phe Tyr Ser Glu Gly Cys Leu Ser Gly Val Pro Val Pro Gly
            100                 105                 110         


Val Trp Leu Val Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu
        115                 120                 125             


Asp Phe Glu Lys Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile
    130                 135                 140                 


Leu Ile Pro Arg Gln Ser Glu Thr Ser Ser Pro Gly Ser Ala 
145                 150                 155              


<210>  4
<211>  238
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein of ompA, human tear lipocalin mutein, 
       albumin-binding domain (abd) and Strep-Tag II

<400>  4

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Gln Ala Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly 
            20                  25                  30          


Thr Trp Tyr Leu Lys Ala Met Thr Val Asp Val Gly Ala Leu Arg Cys 
        35                  40                  45              


Leu Ala Gly Ser Val Ile Pro Thr Thr Leu Thr Thr Leu Glu Gly Gly 
    50                  55                  60                  


Asn Leu Glu Ala Lys Val Thr Met His Ile Lys Gly Arg Ser Gln Glu 
65                  70                  75                  80  


Val Lys Ala Val Leu Ser Lys Thr Asp Glu Pro Gly Ile Tyr Thr Ala 
                85                  90                  95      


Ile Gly Gly Ile His Val Ala Lys Ile Gly Arg Ser His Val Lys Asp 
            100                 105                 110         


His Tyr Ile Phe Tyr Ser Glu Gly Cys Leu Ser Gly Val Pro Val Pro 
        115                 120                 125             


Gly Val Trp Leu Val Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu 
    130                 135                 140                 


Glu Asp Phe Glu Lys Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser 
145                 150                 155                 160 


Ile Leu Ile Pro Arg Gln Ser Glu Thr Ser Ser Pro Gly Ser Ala Gly 
                165                 170                 175     


Ala Val Asp Ala Asn Ser Leu Ala Glu Ala Lys Val Leu Ala Asn Arg 
            180                 185                 190         


Glu Leu Asp Lys Tyr Gly Val Ser Asp Tyr Tyr Lys Asn Leu Ile Asn 
        195                 200                 205             


Asn Ala Lys Thr Val Glu Gly Val Lys Ala Leu Ile Asp Glu Ile Leu 
    210                 215                 220                 


Ala Ala Leu Pro Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
225                 230                 235             


<210>  5
<211>  238
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein of ompA, human tear lipocalin mutein, 
       albumin-binding domain (abd) and Strep-Tag II

<400>  5

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Gln Ala Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly 
            20                  25                  30          


Thr Trp Tyr Leu Lys Ala Met Thr Val Asp Val Gly Ala Leu Arg Cys 
        35                  40                  45              


Leu Ala Gly Ser Val Ile Pro Thr Thr Leu Thr Thr Leu Glu Gly Gly 
    50                  55                  60                  


Asn Leu Glu Ala Lys Val Thr Met His Ile Lys Gly Arg Ser Gln Glu 
65                  70                  75                  80  


Val Lys Ala Val Leu Ser Lys Thr Asp Glu Pro Gly Ile Tyr Thr Ala 
                85                  90                  95      


Ile Gly Gly Ile His Val Ala Lys Ile Gly Arg Ser His Val Lys Cys 
            100                 105                 110         


His Tyr Ile Phe Tyr Ser Glu Gly Cys Leu Ser Gly Val Pro Val Pro 
        115                 120                 125             


Gly Val Trp Leu Val Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu 
    130                 135                 140                 


Glu Asp Phe Glu Lys Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser 
145                 150                 155                 160 


Ile Leu Ile Pro Arg Gln Ser Glu Thr Ser Ser Pro Gly Ser Ala Gly 
                165                 170                 175     


Ala Val Asp Ala Asn Ser Leu Ala Glu Ala Lys Val Leu Ala Asn Arg 
            180                 185                 190         


Glu Leu Asp Lys Tyr Gly Val Ser Asp Tyr Tyr Lys Asn Leu Ile Asn 
        195                 200                 205             


Asn Ala Lys Thr Val Glu Gly Val Lys Ala Leu Ile Asp Glu Ile Leu 
    210                 215                 220                 


Ala Ala Leu Pro Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
225                 230                 235             


<210>  6
<211>  232
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Asn Phe Leu Leu Ser Trp Val His Trp Ser Leu Ala Leu Leu Leu 
1               5                   10                  15      


Tyr Leu His His Ala Lys Trp Ser Gln Ala Ala Pro Met Ala Glu Gly 
            20                  25                  30          


Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr Gln 
        35                  40                  45              


Arg Ser Tyr Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln Glu 
    50                  55                  60                  


Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro Leu 
65                  70                  75                  80  


Met Arg Cys Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val Pro 
                85                  90                  95      


Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Arg Ile Lys Pro His 
            100                 105                 110         


Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys Cys 
        115                 120                 125             


Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Lys Lys Ser Val 
    130                 135                 140                 


Arg Gly Lys Gly Lys Gly Gln Lys Arg Lys Arg Lys Lys Ser Arg Tyr 
145                 150                 155                 160 


Lys Ser Trp Ser Val Tyr Val Gly Ala Arg Cys Cys Leu Met Pro Trp 
                165                 170                 175     


Ser Leu Pro Gly Pro His Pro Cys Gly Pro Cys Ser Glu Arg Arg Lys 
            180                 185                 190         


His Leu Phe Val Gln Asp Pro Gln Thr Cys Lys Cys Ser Cys Lys Asn 
        195                 200                 205             


Thr Asp Ser Arg Cys Lys Ala Arg Gln Leu Glu Leu Asn Glu Arg Thr 
    210                 215                 220                 


Cys Arg Cys Asp Lys Pro Arg Arg 
225                 230         


<210>  7
<211>  207
<212>  PRT
<213>  Homo sapiens

<400>  7

Met Ser Pro Leu Leu Arg Arg Leu Leu Leu Ala Ala Leu Leu Gln Leu 
1               5                   10                  15      


Ala Pro Ala Gln Ala Pro Val Ser Gln Pro Asp Ala Pro Gly His Gln 
            20                  25                  30          


Arg Lys Val Val Ser Trp Ile Asp Val Tyr Thr Arg Ala Thr Cys Gln 
        35                  40                  45              


Pro Arg Glu Val Val Val Pro Leu Thr Val Glu Leu Met Gly Thr Val 
    50                  55                  60                  


Ala Lys Gln Leu Val Pro Ser Cys Val Thr Val Gln Arg Cys Gly Gly 
65                  70                  75                  80  


Cys Cys Pro Asp Asp Gly Leu Glu Cys Val Pro Thr Gly Gln His Gln 
                85                  90                  95      


Val Arg Met Gln Ile Leu Met Ile Arg Tyr Pro Ser Ser Gln Leu Gly 
            100                 105                 110         


Glu Met Ser Leu Glu Glu His Ser Gln Cys Glu Cys Arg Pro Lys Lys 
        115                 120                 125             


Lys Asp Ser Ala Val Lys Pro Asp Arg Ala Ala Thr Pro His His Arg 
    130                 135                 140                 


Pro Gln Pro Arg Ser Val Pro Gly Trp Asp Ser Ala Pro Gly Ala Pro 
145                 150                 155                 160 


Ser Pro Ala Asp Ile Thr His Pro Thr Pro Ala Pro Gly Pro Ser Ala 
                165                 170                 175     


His Ala Ala Pro Ser Thr Thr Ser Ala Leu Thr Pro Gly Pro Ala Ala 
            180                 185                 190         


Ala Ala Ala Asp Ala Ala Ala Ser Ser Val Ala Lys Gly Gly Ala 
        195                 200                 205         


<210>  8
<211>  419
<212>  PRT
<213>  Homo sapiens

<400>  8

Met His Leu Leu Gly Phe Phe Ser Val Ala Cys Ser Leu Leu Ala Ala 
1               5                   10                  15      


Ala Leu Leu Pro Gly Pro Arg Glu Ala Pro Ala Ala Ala Ala Ala Phe 
            20                  25                  30          


Glu Ser Gly Leu Asp Leu Ser Asp Ala Glu Pro Asp Ala Gly Glu Ala 
        35                  40                  45              


Thr Ala Tyr Ala Ser Lys Asp Leu Glu Glu Gln Leu Arg Ser Val Ser 
    50                  55                  60                  


Ser Val Asp Glu Leu Met Thr Val Leu Tyr Pro Glu Tyr Trp Lys Met 
65                  70                  75                  80  


Tyr Lys Cys Gln Leu Arg Lys Gly Gly Trp Gln His Asn Arg Glu Gln 
                85                  90                  95      


Ala Asn Leu Asn Ser Arg Thr Glu Glu Thr Ile Lys Phe Ala Ala Ala 
            100                 105                 110         


His Tyr Asn Thr Glu Ile Leu Lys Ser Ile Asp Asn Glu Trp Arg Lys 
        115                 120                 125             


Thr Gln Cys Met Pro Arg Glu Val Cys Ile Asp Val Gly Lys Glu Phe 
    130                 135                 140                 


Gly Val Ala Thr Asn Thr Phe Phe Lys Pro Pro Cys Val Ser Val Tyr 
145                 150                 155                 160 


Arg Cys Gly Gly Cys Cys Asn Ser Glu Gly Leu Gln Cys Met Asn Thr 
                165                 170                 175     


Ser Thr Ser Tyr Leu Ser Lys Thr Leu Phe Glu Ile Thr Val Pro Leu 
            180                 185                 190         


Ser Gln Gly Pro Lys Pro Val Thr Ile Ser Phe Ala Asn His Thr Ser 
        195                 200                 205             


Cys Arg Cys Met Ser Lys Leu Asp Val Tyr Arg Gln Val His Ser Ile 
    210                 215                 220                 


Ile Arg Arg Ser Leu Pro Ala Thr Leu Pro Gln Cys Gln Ala Ala Asn 
225                 230                 235                 240 


Lys Thr Cys Pro Thr Asn Tyr Met Trp Asn Asn His Ile Cys Arg Cys 
                245                 250                 255     


Leu Ala Gln Glu Asp Phe Met Phe Ser Ser Asp Ala Gly Asp Asp Ser 
            260                 265                 270         


Thr Asp Gly Phe His Asp Ile Cys Gly Pro Asn Lys Glu Leu Asp Glu 
        275                 280                 285             


Glu Thr Cys Gln Cys Val Cys Arg Ala Gly Leu Arg Pro Ala Ser Cys 
    290                 295                 300                 


Gly Pro His Lys Glu Leu Asp Arg Asn Ser Cys Gln Cys Val Cys Lys 
305                 310                 315                 320 


Asn Lys Leu Phe Pro Ser Gln Cys Gly Ala Asn Arg Glu Phe Asp Glu 
                325                 330                 335     


Asn Thr Cys Gln Cys Val Cys Lys Arg Thr Cys Pro Arg Asn Gln Pro 
            340                 345                 350         


Leu Asn Pro Gly Lys Cys Ala Cys Glu Cys Thr Glu Ser Pro Gln Lys 
        355                 360                 365             


Cys Leu Leu Lys Gly Lys Lys Phe His His Gln Thr Cys Ser Cys Tyr 
    370                 375                 380                 


Arg Arg Pro Cys Thr Asn Arg Gln Lys Ala Cys Glu Pro Gly Phe Ser 
385                 390                 395                 400 


Tyr Ser Glu Glu Val Cys Arg Cys Val Pro Ser Tyr Trp Lys Arg Pro 
                405                 410                 415     


Gln Met Ser 
            


<210>  9
<211>  354
<212>  PRT
<213>  Homo sapiens

<400>  9

Met Tyr Arg Glu Trp Val Val Val Asn Val Phe Met Met Leu Tyr Val 
1               5                   10                  15      


Gln Leu Val Gln Gly Ser Ser Asn Glu His Gly Pro Val Lys Arg Ser 
            20                  25                  30          


Ser Gln Ser Thr Leu Glu Arg Ser Glu Gln Gln Ile Arg Ala Ala Ser 
        35                  40                  45              


Ser Leu Glu Glu Leu Leu Arg Ile Thr His Ser Glu Asp Trp Lys Leu 
    50                  55                  60                  


Trp Arg Cys Arg Leu Arg Leu Lys Ser Phe Thr Ser Met Asp Ser Arg 
65                  70                  75                  80  


Ser Ala Ser His Arg Ser Thr Arg Phe Ala Ala Thr Phe Tyr Asp Ile 
                85                  90                  95      


Glu Thr Leu Lys Val Ile Asp Glu Glu Trp Gln Arg Thr Gln Cys Ser 
            100                 105                 110         


Pro Arg Glu Thr Cys Val Glu Val Ala Ser Glu Leu Gly Lys Ser Thr 
        115                 120                 125             


Asn Thr Phe Phe Lys Pro Pro Cys Val Asn Val Phe Arg Cys Gly Gly 
    130                 135                 140                 


Cys Cys Asn Glu Glu Ser Leu Ile Cys Met Asn Thr Ser Thr Ser Tyr 
145                 150                 155                 160 


Ile Ser Lys Gln Leu Phe Glu Ile Ser Val Pro Leu Thr Ser Val Pro 
                165                 170                 175     


Glu Leu Val Pro Val Lys Val Ala Asn His Thr Gly Cys Lys Cys Leu 
            180                 185                 190         


Pro Thr Ala Pro Arg His Pro Tyr Ser Ile Ile Arg Arg Ser Ile Gln 
        195                 200                 205             


Ile Pro Glu Glu Asp Arg Cys Ser His Ser Lys Lys Leu Cys Pro Ile 
    210                 215                 220                 


Asp Met Leu Trp Asp Ser Asn Lys Cys Lys Cys Val Leu Gln Glu Glu 
225                 230                 235                 240 


Asn Pro Leu Ala Gly Thr Glu Asp His Ser His Leu Gln Glu Pro Ala 
                245                 250                 255     


Leu Cys Gly Pro His Met Met Phe Asp Glu Asp Arg Cys Glu Cys Val 
            260                 265                 270         


Cys Lys Thr Pro Cys Pro Lys Asp Leu Ile Gln His Pro Lys Asn Cys 
        275                 280                 285             


Ser Cys Phe Glu Cys Lys Glu Ser Leu Glu Thr Cys Cys Gln Lys His 
    290                 295                 300                 


Lys Leu Phe His Pro Asp Thr Cys Ser Cys Glu Asp Arg Cys Pro Phe 
305                 310                 315                 320 


His Thr Arg Pro Cys Ala Ser Gly Lys Thr Ala Cys Ala Lys His Cys 
                325                 330                 335     


Arg Phe Pro Lys Glu Lys Arg Ala Ala Gln Gly Pro His Ser Arg Lys 
            340                 345                 350         


Asn Pro 




<210>  10
<211>  158
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of human tear lipocalin with binding affinity for VEGF

<400>  10

Leu Leu His His Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr 
1               5                   10                  15      


Trp Tyr Leu Lys Ala Met Thr Val Asp Val Gly Ala Leu Arg Cys Leu 
            20                  25                  30          


Ala Gly Ser Val Ile Pro Thr Thr Leu Thr Thr Leu Glu Gly Gly Asn
        35                  40                  45              


Leu Glu Ala Lys Val Thr Met His Ile Lys Gly Arg Ser Gln Glu Val
    50                  55                  60                  


Lys Ala Val Leu Ser Lys Thr Asp Glu Pro Gly Ile Tyr Thr Ala Ile 
65                  70                  75                  80  


Gly Gly Ile His Val Ala Lys Ile Gly Arg Ser His Val Lys Cys His 
                85                  90                  95      


Tyr Ile Phe Tyr Ser Glu Gly Cys Leu Ser Gly Val Pro Val Pro Gly 
            100                 105                 110         


Val Trp Leu Val Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu
        115                 120                 125             


Asp Phe Glu Lys Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile
    130                 135                 140                 


Leu Ile Pro Arg Gln Ser Glu Thr Ser Ser Pro Gly Ser Asp 
145                 150                 155              


<210>  11
<211>  158
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of human tear lipocalin with binding affinity for VEGF

<400>  11

Leu Leu His His Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr 
1               5                   10                  15      


Trp Tyr Leu Lys Ala Met Thr Val Asp Val Gly Ala Leu Arg Cys Leu 
            20                  25                  30          


Ala Gly Ser Val Ile Pro Thr Thr Leu Thr Thr Leu Glu Gly Gly Asn
        35                  40                  45              


Leu Glu Ala Lys Val Thr Met His Ile Lys Gly Arg Ser Gln Glu Val
    50                  55                  60                  


Lys Ala Val Leu Ser Lys Thr Asp Glu Pro Gly Ile Tyr Thr Ala Ile 
65                  70                  75                  80  


Gly Gly Ile His Val Ala Lys Ile Gly Arg Ser His Val Lys Asp His 
                85                  90                  95      


Tyr Ile Phe Tyr Ser Glu Gly Cys Leu Ser Gly Val Pro Val Pro Gly 
            100                 105                 110         


Val Trp Leu Val Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu
        115                 120                 125             


Asp Phe Glu Lys Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile
    130                 135                 140                 


Leu Ile Pro Arg Gln Ser Glu Thr Ser Ser Pro Gly Ser Asp 
145                 150                 155              


<210>  12
<211>  158
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of human tear lipocalin with binding affinity for VEGF

<400>  12

Leu Leu His His Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr 
1               5                   10                  15      


Trp Tyr Leu Lys Ala Met Thr Val Asp Ser Gly Ala Leu Arg Cys Leu
            20                  25                  30          


Ala Gly Ser Val Ile Pro Thr Thr Leu Thr Thr Leu Glu Gly Gly Asn
        35                  40                  45              


Leu Glu Ala Lys Val Thr Met His Ile Lys Gly Arg Ser Gln Glu Val
    50                  55                  60                  


Lys Ala Val Leu Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Ile 
65                  70                  75                  80  


Gly Gly Ile His Val Ala His Ile Ile Arg Ser His Val Lys Asp His
                85                  90                  95      


Tyr Ile Phe Tyr Ser Glu Gly Cys Leu Ser Gly Val Pro Val Pro Gly
            100                 105                 110         


Val Trp Leu Val Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu
        115                 120                 125             


Asp Phe Glu Lys Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile
    130                 135                 140                 


Leu Ile Pro Arg Gln Ser Glu Thr Ser Ser Pro Gly Ser Asp 
145                 150                 155              


<210>  13
<211>  238
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein of ompA, human tear lipocalin mutein, 
       albumin-binding domain (abd) and Strep-Tag II

<400>  13

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Gln Ala Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly 
            20                  25                  30          


Thr Trp Tyr Leu Lys Ala Met Thr Val Asp Val Gly Ala Leu Arg Cys 
        35                  40                  45              


Leu Ala Gly Ser Val Ile Pro Thr Thr Leu Thr Thr Leu Glu Gly Gly 
    50                  55                  60                  


Asn Leu Glu Ala Lys Val Thr Met His Ile Lys Gly Arg Ser Gln Glu 
65                  70                  75                  80  


Val Lys Ala Val Leu Ser Lys Thr Asp Glu Pro Gly Ile Tyr Thr Ala 
                85                  90                  95      


Ile Gly Gly Ile His Val Ala Lys Ile Gly Arg Ser His Val Lys Asp 
            100                 105                 110         


His Tyr Ile Phe Tyr Ser Glu Gly Cys Leu Ser Gly Val Pro Val Pro 
        115                 120                 125             


Gly Val Trp Leu Val Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu 
    130                 135                 140                 


Glu Asp Phe Glu Lys Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser 
145                 150                 155                 160 


Ile Leu Ile Pro Arg Gln Ser Glu Thr Ser Ser Pro Gly Ser Asp Gly 
                165                 170                 175     


Ala Val Asp Ala Asn Ser Leu Ala Glu Ala Lys Val Leu Ala Asn Arg 
            180                 185                 190         


Glu Leu Asp Lys Tyr Gly Val Ser Asp Tyr Tyr Lys Asn Leu Ile Asn 
        195                 200                 205             


Asn Ala Lys Thr Val Glu Gly Val Lys Ala Leu Ile Asp Glu Ile Leu 
    210                 215                 220                 


Ala Ala Leu Pro Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
225                 230                 235             


<210>  14
<211>  238
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein of ompA, human tear lipocalin mutein, 
       albumin-binding domain (abd) and Strep-Tag II

<400>  14

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Gln Ala Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly 
            20                  25                  30          


Thr Trp Tyr Leu Lys Ala Met Thr Val Asp Val Gly Ala Leu Arg Cys 
        35                  40                  45              


Leu Ala Gly Ser Val Ile Pro Thr Thr Leu Thr Thr Leu Glu Gly Gly 
    50                  55                  60                  


Asn Leu Glu Ala Lys Val Thr Met His Ile Lys Gly Arg Ser Gln Glu 
65                  70                  75                  80  


Val Lys Ala Val Leu Ser Lys Thr Asp Glu Pro Gly Ile Tyr Thr Ala 
                85                  90                  95      


Ile Gly Gly Ile His Val Ala Lys Ile Gly Arg Ser His Val Lys Cys 
            100                 105                 110         


His Tyr Ile Phe Tyr Ser Glu Gly Cys Leu Ser Gly Val Pro Val Pro 
        115                 120                 125             


Gly Val Trp Leu Val Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu 
    130                 135                 140                 


Glu Asp Phe Glu Lys Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser 
145                 150                 155                 160 


Ile Leu Ile Pro Arg Gln Ser Glu Thr Ser Ser Pro Gly Ser Asp Gly 
                165                 170                 175     


Ala Val Asp Ala Asn Ser Leu Ala Glu Ala Lys Val Leu Ala Asn Arg 
            180                 185                 190         


Glu Leu Asp Lys Tyr Gly Val Ser Asp Tyr Tyr Lys Asn Leu Ile Asn 
        195                 200                 205             


Asn Ala Lys Thr Val Glu Gly Val Lys Ala Leu Ile Asp Glu Ile Leu 
    210                 215                 220                 


Ala Ala Leu Pro Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
225                 230                 235             


<210>  15
<211>  176
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc
<222>  (1)..(176)
<223>  Human tear lipocalin

<400>  15

Met Lys Pro Leu Leu Leu Ala Val Ser Leu Gly Leu Ile Ala Ala Leu 
1               5                   10                  15      


Gln Ala His His Leu Leu Ala Ser Asp Glu Glu Ile Gln Asp Val Ser 
            20                  25                  30          


Gly Thr Trp Tyr Leu Lys Ala Met Thr Val Asp Arg Glu Phe Pro Glu 
        35                  40                  45              


Met Asn Leu Glu Ser Val Thr Pro Met Thr Leu Thr Thr Leu Glu Gly 
    50                  55                  60                  


Gly Asn Leu Glu Ala Lys Val Thr Met Leu Ile Ser Gly Arg Cys Gln 
65                  70                  75                  80  


Glu Val Lys Ala Val Leu Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr 
                85                  90                  95      


Ala Asp Gly Gly Lys His Val Ala Tyr Ile Ile Arg Ser His Val Lys 
            100                 105                 110         


Asp His Tyr Ile Phe Tyr Cys Glu Gly Glu Leu His Gly Lys Pro Val 
        115                 120                 125             


Arg Gly Val Lys Leu Val Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala 
    130                 135                 140                 


Leu Glu Asp Phe Glu Lys Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu 
145                 150                 155                 160 


Ser Ile Leu Ile Pro Arg Gln Ser Glu Thr Cys Ser Pro Gly Ser Asp 
                165                 170                 175     


<210>  16
<211>  158
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc
<222>  (1)..(158)
<223>  Mature human lear lipocalin

<400>  16

His His Leu Leu Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr 
1               5                   10                  15      


Trp Tyr Leu Lys Ala Met Thr Val Asp Arg Glu Phe Pro Glu Met Asn 
            20                  25                  30          


Leu Glu Ser Val Thr Pro Met Thr Leu Thr Thr Leu Glu Gly Gly Asn 
        35                  40                  45              


Leu Glu Ala Lys Val Thr Met Leu Ile Ser Gly Arg Cys Gln Glu Val 
    50                  55                  60                  


Lys Ala Val Leu Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Asp 
65                  70                  75                  80  


Gly Gly Lys His Val Ala Tyr Ile Ile Arg Ser His Val Lys Asp His 
                85                  90                  95      


Tyr Ile Phe Tyr Cys Glu Gly Glu Leu His Gly Lys Pro Val Arg Gly 
            100                 105                 110         


Val Lys Leu Val Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu 
        115                 120                 125             


Asp Phe Glu Lys Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile 
    130                 135                 140                 


Leu Ile Pro Arg Gln Ser Glu Thr Cys Ser Pro Gly Ser Asp 
145                 150                 155             
