                         SEQUENCE LISTING

<110>  University of Manitoba
 
<120>  INHIBITION OF COLLAGEN SYNTHESIS

<130>  V84465WO

<140>  Not known
<141>  2012-11-05

<150>  US 61/555,735
<151>  2011-11-04

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  207
<212>  PRT
<213>  Mus musculus

<400>  1

Met Ser Phe Ala Met Leu Arg Ser Ala Pro Pro Pro Gly Arg Tyr Leu 
1               5                   10                  15      


Tyr Pro Glu Val Ser Pro Leu Ser Glu Asp Glu Asp Arg Gly Ser Glu 
            20                  25                  30          


Ser Ser Gly Ser Asp Glu Lys Pro Cys Arg Val His Ala Ala Arg Cys 
        35                  40                  45              


Gly Leu Gln Gly Ala Arg Arg Arg Ala Gly Gly Arg Arg Ala Ala Gly 
    50                  55                  60                  


Ser Gly Pro Gly Pro Gly Gly Arg Pro Gly Arg Glu Pro Arg Gln Arg 
65                  70                  75                  80  


His Thr Ala Asn Ala Arg Glu Arg Asp Arg Thr Asn Ser Val Asn Thr 
                85                  90                  95      


Ala Phe Thr Ala Leu Arg Thr Leu Ile Pro Thr Glu Pro Ala Asp Arg 
            100                 105                 110         


Lys Leu Ser Lys Ile Glu Thr Leu Arg Leu Ala Ser Ser Tyr Ile Ser 
        115                 120                 125             


His Leu Gly Asn Val Leu Leu Val Gly Glu Ala Cys Gly Asp Gly Gln 
    130                 135                 140                 


Pro Cys His Ser Gly Pro Ala Phe Phe His Ser Gly Arg Ala Gly Ser 
145                 150                 155                 160 


Pro Leu Pro Pro Pro Pro Pro Pro Pro Pro Leu Ala Arg Asp Gly Gly 
                165                 170                 175     


Glu Asn Thr Gln Pro Lys Gln Ile Cys Thr Phe Cys Leu Ser Asn Gln 
            180                 185                 190         


Arg Lys Leu Ser Lys Asp Arg Asp Arg Lys Thr Ala Ile Arg Ser 
        195                 200                 205         


<210>  2
<211>  209
<212>  PRT
<213>  Rattus norvegicus

<400>  2

Met Ser Phe Ala Met Leu Arg Ser Ala Pro Pro Pro Gly Arg Tyr Leu 
1               5                   10                  15      


Tyr Pro Glu Val Ser Pro Leu Ser Glu Asp Glu Asp Arg Gly Ser Glu 
            20                  25                  30          


Ser Ser Gly Ser Asp Glu Lys Pro Cys Arg Val His Ala Ala Arg Cys 
        35                  40                  45              


Gly Leu Gln Gly Ala Arg Arg Arg Ala Gly Gly Arg Arg Ala Ala Gly 
    50                  55                  60                  


Ser Gly Pro Gly Pro Gly Gly Arg Pro Gly Arg Glu Pro Arg Gln Arg 
65                  70                  75                  80  


His Thr Ala Asn Ala Arg Glu Arg Asp Arg Thr Asn Ser Val Asn Thr 
                85                  90                  95      


Ala Phe Thr Ala Leu Arg Thr Leu Ile Pro Thr Glu Pro Ala Asp Arg 
            100                 105                 110         


Lys Leu Ser Lys Ile Glu Thr Leu Arg Leu Ala Ser Ser Tyr Ile Ser 
        115                 120                 125             


His Leu Gly Asn Val Leu Leu Val Gly Glu Ala Cys Gly Asp Gly Gln 
    130                 135                 140                 


Pro Cys His Ser Gly Pro Ala Phe Phe His Ser Gly Arg Ala Gly Ser 
145                 150                 155                 160 


Pro Leu Pro Pro Pro Pro Pro Pro Pro Pro Leu Pro Leu Ala Arg Asp 
                165                 170                 175     


Gly Gly Glu Asn Thr Gln Pro Lys Gln Ile Cys Thr Phe Cys Leu Ser 
            180                 185                 190         


Asn Gln Arg Lys Leu Ser Lys Asp Arg Asp Arg Lys Thr Ala Ile Arg 
        195                 200                 205             


Ser 
    


<210>  3
<211>  201
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Ser Phe Ala Thr Leu Arg Pro Ala Pro Pro Gly Arg Tyr Leu Tyr 
1               5                   10                  15      


Pro Glu Val Ser Pro Leu Ser Glu Asp Glu Asp Arg Gly Ser Asp Ser 
            20                  25                  30          


Ser Gly Ser Asp Glu Lys Pro Cys Arg Val His Ala Ala Arg Cys Gly 
        35                  40                  45              


Leu Gln Gly Ala Arg Arg Arg Ala Gly Gly Arg Arg Ala Gly Gly Gly 
    50                  55                  60                  


Gly Pro Gly Gly Arg Pro Gly Arg Glu Pro Arg Gln Arg His Thr Ala 
65                  70                  75                  80  


Asn Ala Arg Glu Arg Asp Arg Thr Asn Ser Val Asn Thr Ala Phe Thr 
                85                  90                  95      


Ala Leu Arg Thr Leu Ile Pro Thr Glu Pro Ala Asp Arg Lys Leu Ser 
            100                 105                 110         


Lys Ile Glu Thr Leu Arg Leu Ala Ser Ser Tyr Ile Ser His Leu Gly 
        115                 120                 125             


Asn Val Leu Leu Ala Gly Glu Ala Cys Gly Asp Gly Gln Pro Cys His 
    130                 135                 140                 


Ser Gly Pro Ala Phe Phe His Ala Ala Arg Ala Gly Ser Pro Pro Pro 
145                 150                 155                 160 


Pro Pro Pro Pro Pro Pro Ala Arg Asp Gly Glu Asn Thr Gln Pro Lys 
                165                 170                 175     


Gln Ile Cys Thr Phe Cys Leu Ser Asn Gln Arg Lys Leu Ser Lys Asp 
            180                 185                 190         


Arg Asp Arg Lys Thr Ala Ile Arg Ser 
        195                 200     


<210>  4
<211>  624
<212>  DNA
<213>  Mus musculus

<400>  4
atgtccttcg ccatgctgcg ttcagcgccg ccgccgggtc gctacctgta ccctgaggtg       60

agcccgctgt cggaggatga ggaccgcgga agcgagagct cgggctccga cgagaaaccc      120

tgccgtgtgc atgctgcgcg ctgtggcctc cagggcgccc ggcggcgggc aggaggacgg      180

agggccgcgg gtagcgggcc aggacccggg gggcggccag gccgcgagcc ccggcagcgg      240

cacacagcga atgcgcgcga gcgggaccgc accaacagcg tgaacacggc cttcactgcg      300

ctgcgcacac tcatccccac cgagccagcg gaccgcaagc tctccaagat tgagacgctg      360

cgcctggcct ccagctacat ttctcacctg ggcaatgtgc tgctggtggg tgaggcctgt      420

ggcgacgggc aaccatacat ttctcacctg ggcaatgtgc tgctggtggg tgaggcctgt      480

ggcgacgggc aaccatcgcc gccaccacca ctggccagag acggcggcga gaacacccag      540

cccaaacaga tctgcacgcc gccaccacca ctggccagag acggcggcga gaacacccag      600

cccaaacaga tctgcagaag ttag                                             624


<210>  5
<211>  585
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Scleraxis nucleotide sequence with a deleted binding domain (the 
       scleraxis basic domain deletion mutant)

<400>  5
atgtccttcg ccatgctgcg ttcagcgccg ccgccgggtc gctacctgta ccctgaggtg       60

agcccgctgt cggaggatga ggaccgcgga agcgagagct cgggctccga cgagaaaccc      120

tgccgtgtgc atgctgcgcg ctgtggcctc cagggcgccc ggcggcgggc aggaggacgg      180

agggccgcgg gtagcgggcc aggacccggg gggcggccag gccgcgagcc caccaacagc      240

gtgaacacgg ccttcactgc gctgcgcaca ctcatcccca ccgagccagc ggaccgcaag      300

ctctccaaga ttgagacgct gcgcctggcc tccagctaca tttctcacct gggcaatgtg      360

ctgctggtgg gtgaggcctg tggcgacggg caaccataca tttctcacct gggcaatgtg      420

ctgctggtgg gtgaggcctg tggcgacggg caaccatcgc cgccaccacc actggccaga      480

gacggcggcg agaacaccca gcccaaacag atctgcacgc cgccaccacc actggccaga      540

gacggcggcg agaacaccca gcccaaacag atctgcagaa gttag                      585


<210>  6
<211>  705
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse scleraxis wild type nucleotide sequence with inserted FLAG 
       and HA sequences

<400>  6
atggattaca aggacgacga cgataagatc tgtcgacggt accccgggga attctatccg       60

tatgatgtgc cggattatgc gatgtccttc gccatgctgc gttcagcgcc gccgccgggt      120

cgctacctgt accctgaggt gagcccgctg tcggaggatg aggaccgcgg aagcgagagc      180

tcgggctccg acgagaaacc ctgccgtgtg catgctgcgc gctgtggcct ccagggcgcc      240

cggcggcggg caggaggacg gagggccgcg ggtagcgggc caggacccgg ggggcggcca      300

ggccgcgagc cccggcagcg gcacacagcg aatgcgcgcg agcgggaccg caccaacagc      360

gtgaacacgg ccttcactgc gctgcgcaca ctcatcccca ccgagccagc ggaccgcaag      420

ctctccaaga ttgagacgct gcgcctggcc tccagctaca tttctcacct gggcaatgtg      480

ctgctggtgg gtgaggcctg tggcgacggg caaccataca tttctcacct gggcaatgtg      540

ctgctggtgg gtgaggcctg tggcgacggg caaccatcgc cgccaccacc actggccaga      600

gacggcggcg agaacaccca gcccaaacag atctgcacgc cgccaccacc actggccaga      660

gacggcggcg agaacaccca gcccaaacag atctgcagaa gttag                      705


<210>  7
<211>  666
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse scleraxis basic domain deletion mutant with inserted FLAG 
       and HA sequences

<400>  7
atggattaca aggacgacga cgataagatc tgtcgacggt accccgggga attctatccg       60

tatgatgtgc cggattatgc gatgtccttc gccatgctgc gttcagcgcc gccgccgggt      120

cgctacctgt accctgaggt gagcccgctg tcggaggatg aggaccgcgg aagcgagagc      180

tcgggctccg acgagaaacc ctgccgtgtg catgctgcgc gctgtggcct ccagggcgcc      240

cggcggcggg caggaggacg gagggccgcg ggtagcgggc caggacccgg ggggcggcca      300

ggccgcgagc ccaccaacag cgtgaacacg gccttcactg cgctgcgcac actcatcccc      360

accgagccag cggaccgcaa gctctccaag attgagacgc tgcgcctggc ctccagctac      420

atttctcacc tgggcaatgt gctgctggtg ggtgaggcct gtggcgacgg gcaaccatac      480

atttctcacc tgggcaatgt gctgctggtg ggtgaggcct gtggcgacgg gcaaccatcg      540

ccgccaccac cactggccag agacggcggc gagaacaccc agcccaaaca gatctgcacg      600

ccgccaccac cactggccag agacggcggc gagaacaccc agcccaaaca gatctgcaga      660

agttag                                                                 666


<210>  8
<211>  194
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein expressed by the mouse scleraxis basic domain deletion 
       mutant with inserted FLAG and HA sequences

<400>  8

Met Ser Phe Ala Met Leu Arg Ser Ala Pro Pro Pro Gly Arg Tyr Leu 
1               5                   10                  15      


Tyr Pro Glu Val Ser Pro Leu Ser Glu Asp Glu Asp Arg Gly Ser Glu 
            20                  25                  30          


Ser Ser Gly Ser Asp Glu Lys Pro Cys Arg Val His Ala Ala Arg Cys 
        35                  40                  45              


Gly Leu Gln Gly Ala Arg Arg Arg Ala Gly Gly Arg Arg Ala Ala Gly 
    50                  55                  60                  


Ser Gly Pro Gly Pro Gly Gly Arg Pro Gly Arg Glu Pro Thr Asn Ser 
65                  70                  75                  80  


Val Asn Thr Ala Phe Thr Ala Leu Arg Thr Leu Ile Pro Thr Glu Pro 
                85                  90                  95      


Ala Asp Arg Lys Leu Ser Lys Ile Glu Thr Leu Arg Leu Ala Ser Ser 
            100                 105                 110         


Tyr Ile Ser His Leu Gly Asn Val Leu Leu Val Gly Glu Ala Cys Gly 
        115                 120                 125             


Asp Gly Gln Pro Cys His Ser Gly Pro Ala Phe Phe His Ser Gly Arg 
    130                 135                 140                 


Ala Gly Ser Pro Leu Pro Pro Pro Pro Pro Pro Pro Pro Leu Ala Arg 
145                 150                 155                 160 


Asp Gly Gly Glu Asn Thr Gln Pro Lys Gln Ile Cys Thr Phe Cys Leu 
                165                 170                 175     


Ser Asn Gln Arg Lys Leu Ser Lys Asp Arg Asp Arg Lys Thr Ala Ile 
            180                 185                 190         


Arg Ser 
        


